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Variability and availability of water resources under changing environment in a regional scale have been hot topics in recent years, due to the vulnerability of water resources associated with social and economic development. In this paper, four subbasins in the Dongjiang basin with a significant land use change were selected as case study. Runoffs of the four subbasins were simulated using the SCS monthly model to identify the quantitative impacts of land use and climate change. The results showed that (1), in the Dongjiang basin, temperature increased significantly, evaporation and sunlight decreased strongly, while precipitation showed a nonsignificant increase; (2) since the 1980s, land uses in the Dongjiang basin have experienced a significant change with a prominent increase inurbanareas, amoderate increase in farmlands, anda greatdecrease inforest areas; (3) the SCSmonthlymodel performed well in the four subbasins giving that the more significant land use change in each subbasin, the more runoff change correspondingly; (4) overall, runoff change was contributed half and half by climate change and human activities, respectively, in all the subbasins, in which about 20%~30% change was contributed by land use change. [ABSTRACT FROM AUTHOR]

• The rainfall-runoff relationship has changed under climate change and human activities in the TRH region. • The impacts climate indicators of rainfall-runoff ratio in the sub-basins of TRH region were different. • The contribution rates of human activity in the YRB were a little higher than that in the YARB. Identifying the impact of climate change and human activities on rainfall-runoff relationship in the Three-River Headwaters (TRH) region is essential for water source conservation, ecological security, and biodiversity maintenance of China and East Asia. In this paper, the runoff-rainfall ratio was used as the index of rainfall-runoff relationship. Different methods were employed to identify the impact indicators and quantitative assessment of rainfall-runoff relationship from 1957 to 2016. The results are the following: (1) the rainfall-runoff relationship has changed in the source regions of Yangtze River basin (YARB) and Yellow River (YRB), and runoff-rainfall ratio has increased by 2.9% and decreased by 3.2%, respectively; (2) the main climate indicators of runoff-rainfall ratio were rainfall, potential evaporation and sunshine hours in the YARB, and rainfall, potential evaporation and temperature in the YRB; (3) the contribution rates of human activity in the YRB were a little higher than that in the YARB, and the contribution rates of climate change and human activity were 90.9% and 9.1% in the YARB and 42.9% and 57.1% in the YRB. The results can provide invaluable information for managing water resources and taking ecological protection measures. [ABSTRACT FROM AUTHOR]