1. Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses.
- Author
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Lin S, Wang S, Cheng X, Xiao S, Chen X, Chen S, Chen S, and Yu F
- Subjects
- Animals, Benzothiazoles, Cloaca virology, Diamines, Genome, Viral, Organic Chemicals, Oropharynx virology, Parvoviridae Infections diagnosis, Parvovirus isolation & purification, Phylogeny, Poultry Diseases virology, Quinolines, Transition Temperature, Viral Load, Ducks virology, Geese virology, Parvoviridae Infections veterinary, Parvovirus classification, Poultry Diseases diagnosis, Real-Time Polymerase Chain Reaction methods
- Abstract
Background: Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important., Results: A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded., Conclusion: This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.
- Published
- 2019
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