1. AFM visualization of cortical filaments/network under cell-bound membrane vesicles.
- Author
-
Zhang, Xiaojun, Tang, Qisheng, Wu, Li, Huang, Jie, and Chen, Yong
- Subjects
- *
CYTOPLASMIC filaments , *CELL membranes , *CYTOSKELETON , *TUMOR necrosis factors , *CONFOCAL microscopy , *ATOMIC force microscopy - Abstract
While circulating/plasma membrane vesicles have been extensively characterized, due to the lack of effective methods cell-bound membrane vesicles are poorly understood including their shape and correlation with the intracellular cytoskeleton. In this study, we focused on cell-bound membrane vesicles and individual vesicle-derived pits on endothelial cells by using confocal microscopy and atomic force microscopy (AFM). For the first time, we found that cell-bound membrane vesicles are hemisphere-shaped and that the actin cortical filaments/network lies at the cytosolic opening of a vesicle instead of being closely attached to the inner side of the vesicle membrane. This structure of cell-bound membrane vesicles may be beneficial to their movement in, or release from, the plasma membrane of cells due to less membrane–cytoskeleton coupling to be broken therefore probably minimizing energy consumption and time usage. Further study indicates that TNF-α activation induced a significant increase in average number/size of cell-bound vesicles and the local disruption of the actin network at the cytosolic opening of cell-bound vesicles. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF