1. Exploring the oncogenic potential of circSOD2 in clear cell renal cell carcinoma: a novel positive feedback loop.
- Author
-
Yao GS, Fu LM, Dai JS, Chen JW, Liu KZ, Liang H, Wang Z, Deng Q, Wang JY, Jin MY, Chen W, Fang Y, Luo JH, Cao JZ, and Wei JH
- Subjects
- Humans, Cell Line, Tumor, Female, Middle Aged, Male, Carcinogenesis genetics, Carcinogenesis pathology, Cell Movement genetics, PAX5 Transcription Factor metabolism, PAX5 Transcription Factor genetics, Oncogenes genetics, Base Sequence, Disease Progression, Neoplasm Invasiveness, Reproducibility of Results, Carcinoma, Renal Cell genetics, Carcinoma, Renal Cell pathology, Carcinoma, Renal Cell metabolism, RNA, Circular genetics, RNA, Circular metabolism, Kidney Neoplasms genetics, Kidney Neoplasms pathology, Kidney Neoplasms metabolism, MicroRNAs genetics, MicroRNAs metabolism, Feedback, Physiological, Gene Expression Regulation, Neoplastic, Cell Proliferation genetics
- Abstract
Background: Existing studies have found that circular RNAs (circRNAs) act as sponges for micro RNAs (miRNAs) to control downstream genes. However, the specific functionalities and mechanisms of circRNAs in human clear cell renal cell carcinoma (ccRCC) have yet to be thoroughly investigated., Methods: Patient cohorts from online databases were used to screen candidate circRNAs, while another cohort from our hospital was obtained for validation. CircSOD2 was identified as a potential oncogenic target, and its relevant characteristics were investigated during ccRCC progression through various assays. A positive feedback loop containing downstream miRNA and its target gene were identified using bioinformatics and validated by luciferase reporter assays, RNA pull-down, and high-throughput sequencing., Results: CircSOD2 expression was elevated in tumor samples and significantly correlated with overall survival (OS) and the tumor stage of ccRCC patients, which appeared in the enhanced proliferation, invasion, and migration of tumor cells. Through competitive binding to circSOD2, miR-532-3p can promote the expression of PAX5 and the progression of ccRCC, and such regulation can be salvaged by miR-532-3p inhibitor., Conclusion: A novel positive feedback loop, PAX5/circSOD2/miR-532-3p/PAX5 was identified in the study, indicating that the loop may play an important role in the diagnosis and prognostic prediction in ccRCC patients., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF