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The relationships amongst modern pollen assemblages, vegetation, climate and human activity are the basis for reconstructing palaeoenvironmental changes using pollen records. It is important to determine these relationships at regional scales due to the development of vegetation under different climatic conditions and human activities. In this paper, we report on an analysis of modern pollen assemblages of 31 surface lake samples from 31 lakes (one sample per lake) on the southwestern Tibetan Plateau where the knowledge of modern pollen and their relationships with vegetation, climate and human activities is insufficient. The region includes five vegetation zones: sub-alpine shrub steppe, alpine steppe, alpine meadow and steppe ecotone, mountain desert and alpine desert. The lakes span a wide range of mean annual precipitation (50-500 mm) and mean annual temperature (−8 to 6 °C). Modern pollen assemblages from our samples mainly consist of herb taxa ( Artemisia, Cyperaceae, Poaceae, Chenopodiaceae, etc.) and some tree taxa ( Pinus, Fagaceae, Alnus, etc.). The results indicate that modern pollen assemblages are able to reflect the main vegetation distribution. Redundancy analysis for the main pollen types and environmental variables shows that precipitation is the leading factor that influences the pollen distribution in the study area with the first axis capturing 13.7% of the variance in the pollen data set. The Artemisia/Chenopodiaceae ratio is valid for separating the desert component (<2) from the steppe and other vegetation zones (>2) but is unable to distinguish moisture variations. The Artemisia/Cyperaceae ratio is able to identify meadows (<1) and steppes (>1) and can be used as a moisture index on the southwestern Tibetan Plateau. Our results show that an appropriate range is needed for a modern pollen data set in order to perform pollen-based quantitative climate reconstructions in one region. It is essential to perform modern studies before using pollen ratios to reconstruct palaeovegetation and palaeoclimate at a regional scale. [ABSTRACT FROM AUTHOR]