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Herein we have reported a fluorescent probe (MB-M) based on MB derivative for Cu2+ ions detection. The probe was well characterized by 1H NMR, 13C NMR and HR-MS spectrum. Probe MB-M showed naked-eyes recognition to Cu2+ as color change from colorless to indigo. The probe exhibited promising features such as high fluorescence and UV–vis selectivity, fast response (5 mint), workable at pH 2–7, and low limit of detection (LOD = 0.33 µM). Probe MB-M was also used for Cu2+ ions imaging in HepG-2 cells and detection in daily life (Test Strip and lake water). Moreover, non-covalent interaction (NCI) and quantum theory of atoms in molecules (QTAIM) analysis were used to study the interaction between MB-M and Cu2+ ions. By examining the electronic characteristics of the complex using natural bond orbital (NBO), electron density difference (EDD), and frontier molecular orbital (FMO) analysis, the sensitivity of MB-M towards Cu2+ ions were investigated. The results illustrated that the interactions between MB-M and Cu2+ ions involved chemisorption. [ABSTRACT FROM AUTHOR]

A new rhodamine 6G derived chemosensor L has been synthesized that selectively detects the presence of Cu 2+ ion over a host of other biologically important and environmentally relevant metal ions in aqueous alcoholic medium under physiological pH (ethanol: water, 1:1, v/v, pH 7.2). The significant enhancement of fluorescence intensity of L is caused by Cu 2+ ion induced cleavage of spirolactum bond in the rhodamine moiety. This mechanism is well supported by the crystal structure of the L-Cu 2+ complex that depicts two ligand units are bonded to two Cu 2+ ions. Color of the solution changes from light orange to dark pink upon metal binding which allows naked eye detection ion as well. Confocal microscopic experiments validate that the dye is potentially useful for the selective detection of Cu 2+ ion in living cells. Therefore the dye is an excellent fluorogenic and chromogenic chemosensor for the Cu 2+ ion in aqueous ethanolic medium and the living cell as well. [ABSTRACT FROM AUTHOR]