1. Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo.
- Author
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Kutzler, M. A., Kraynyak, K. A., Nagle, S. J., Parkinson, R. M., Zharikova, D., Chattergoon, M., Maguire, H., Muthumani, K., Ugen, K., and Weiner, D. B.
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PLASMIDS , *CYTOPLASMIC inheritance , *DNA , *PREVENTIVE medicine , *VACCINATION , *DNA vaccines , *PREVENTION of communicable diseases - Abstract
A hurdle facing DNA vaccine development is the ability to generate strong immune responses systemically and at local immune sites. We report a novel systemically administered DNA vaccination strategy using intramuscular codelivery of CCL27 or CCL28, which elicited elevated peripheral IFN-γ and antigen-specific IgG while driving antigen-specific T-cell secretion of cytokine and antibody production in the gut-associated lymphoid tissue and lung. This strategy resulted in induction of long-lived antibody responses that neutralized influenza A/PR8/34 and protected mice from morbidity and mortality associated with a lethal intranasal viral challenge. This is the first example of the use of CCL27 and CCL28 chemokines as adjuvants to influence a DNA vaccine strategy, suggesting further examination of this approach for manipulation of vaccine-induced immunity impacting both quality and phenotype of responses. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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