1. Diffusional mobility of Golgi proteins in membranes of living cells
- Author
-
Jennifer Lippincott-Schwartz, Michael Edidin, Noah Sciaky, Mark Terasaki, Nelson B. Cole, and Carolyn L. Smith
- Subjects
Receptors, Peptide ,Endosome ,Recombinant Fusion Proteins ,Green Fluorescent Proteins ,Golgi Apparatus ,Biology ,Endoplasmic Reticulum ,Green fluorescent protein ,Diffusion ,symbols.namesake ,Fluorides ,Mannosidases ,N-Acetyllactosamine Synthase ,Humans ,Aluminum Compounds ,Secretory pathway ,Golgi membrane ,Multidisciplinary ,Microscopy, Confocal ,Membrane Proteins ,Golgi Targeting ,Intracellular Membranes ,Golgi apparatus ,Fusion protein ,Cell biology ,Luminescent Proteins ,Membrane ,Mutation ,symbols ,HeLa Cells - Abstract
The mechanism by which Golgi membrane proteins are retained within the Golgi complex in the midst of a continuous flow of protein and lipid is not yet understood. The diffusional mobilities of mammalian Golgi membrane proteins fused with green fluorescent protein from Aequorea victoria were measured in living HeLa cells with the fluorescence photobleaching recovery technique. The diffusion coefficients ranged from 3 × 10 −9 square centimeters per second to 5 × 10 −9 square centimeters per second, with greater than 90 percent of the chimeric proteins mobile. Extensive lateral diffusion of the chimeric proteins occurred between Golgi stacks. Thus, the chimeras diffuse rapidly and freely in Golgi membranes, which suggests that Golgi targeting and retention of these molecules does not depend on protein immobilization.
- Published
- 1996