1. Maize Tricin-Oligolignol Metabolites and Their Implications for Monocot Lignification.
- Author
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Lan W, Morreel K, Lu F, Rencoret J, Carlos Del Río J, Voorend W, Vermerris W, Boerjan W, and Ralph J
- Subjects
- Acylation, Biosynthetic Pathways, Cell Wall chemistry, Cell Wall metabolism, Flavones chemistry, Flavonoids chemistry, Lignin chemistry, Polymers chemistry, Polymers metabolism, Zea mays chemistry, Flavones metabolism, Flavonoids metabolism, Lignin metabolism, Zea mays metabolism
- Abstract
Lignin is an abundant aromatic plant cell wall polymer consisting of phenylpropanoid units in which the aromatic rings display various degrees of methoxylation. Tricin [5,7-dihydroxy-2-(4-hydroxy-3,5-dimethoxyphenyl)-4H-chromen-4-one], a flavone, was recently established as a true monomer in grass lignins. To elucidate the incorporation pathways of tricin into grass lignin, the metabolites of maize (Zea mays) were extracted from lignifying tissues and profiled using the recently developed 'candidate substrate product pair' algorithm applied to ultra-high-performance liquid chromatography and Fourier transform-ion cyclotron resonance-mass spectrometry. Twelve tricin-containing products (each with up to eight isomers), including those derived from the various monolignol acetate and p-coumarate conjugates, were observed and authenticated by comparisons with a set of synthetic tricin-oligolignol dimeric and trimeric compounds. The identification of such compounds helps establish that tricin is an important monomer in the lignification of monocots, acting as a nucleation site for starting lignin chains. The array of tricin-containing products provides further evidence for the combinatorial coupling model of general lignification and supports evolving paradigms for the unique nature of lignification in monocots., (© 2016 American Society of Plant Biologists. All Rights Reserved.)
- Published
- 2016
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