11 results on '"Shah, Pankaj"'
Search Results
2. Enteral infusion of glucose at rates approximating EGP enhances glucose disposal but does not cause hypoglycemia.
- Author
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Zangeneh F, Basu R, Shah P, Arora P, Camilleri M, and Rizza RA
- Subjects
- Adult, C-Peptide blood, Deuterium, Duodenum, Fasting, Female, Glucagon blood, Glucose pharmacokinetics, Humans, Infusions, Intravenous, Insulin blood, Kinetics, Male, Tritium, Blood Glucose analysis, Blood Glucose metabolism, Glucose administration & dosage, Intubation, Gastrointestinal
- Abstract
Portal infusion of glucose at rates approximating endogenous glucose production (EGP) causes paradoxical hypoglycemia in wild-type but not GLUT2 null mice, implying activation of a specific portal glucose sensor. To determine whether this occurs in humans, glucose containing [3-3H]glucose was infused intraduodenally at rates of 3.1 mg. kg-1. min-1 (n = 5), 1.55 mg. kg-1. min-1 (n = 9), or 0/0.1 mg. kg-1. min-1 (n = 9) for 7 h in healthy nondiabetic subjects. [6,6-2H2]glucose was infused intravenously to enable simultaneous measurement of EGP, glucose disappearance, and the rate of appearance of the intraduodenally infused glucose. Plasma glucose concentrations fell (P < 0.01) from 90 +/- 1 to 84 +/- 2 mg/dl during the 0/0.1 mg. kg-1. min-1 id infusions but increased (P < 0.001) to 104 +/- 5 and 107 +/- 3 mg/dl, respectively, during the 1.55 and 3.1 mg. kg-1. min-1 id infusions. In contrast, insulin increased (P < 0.05) during the 1.55 and 3.0 mg. kg-1. min-1 infusions, reaching a peak of 10 +/- 2 and 18 +/- 5 micro U/ml, respectively, by 2 h. Insulin concentrations then fell back to concentrations that no longer differed by study end (7 +/- 1 vs. 8 +/- 1 micro U/ml). This resulted in comparable suppression of EGP by study end (0.84 +/- 0.2 and 0.63 +/- 0.1 mg. kg-1. min-1). Glucose disappearance was higher (P < 0.01) during the final hour of the 3.1 than 1.55 mg. kg-1. min-1 id infusion (4.47 +/- 0.2 vs. 2.6 +/- 0.1 mg. kg-1. min-1), likely because of the slightly, but not significantly, higher glucose and insulin concentrations. We conclude that, in contrast to mice, selective portal glucose delivery at rates approximating EGP does not cause hypoglycemia in humans.
- Published
- 2003
- Full Text
- View/download PDF
3. Elevated free fatty acids impair glucose metabolism in women: decreased stimulation of muscle glucose uptake and suppression of splanchnic glucose production during combined hyperinsulinemia and hyperglycemia.
- Author
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Shah P, Vella A, Basu A, Basu R, Adkins A, Schwenk WF, Johnson CM, Nair KS, Jensen MD, and Rizza RA
- Subjects
- Adult, C-Peptide blood, Female, Glucose administration & dosage, Glucose pharmacokinetics, Glucose pharmacology, Glycerol blood, Human Growth Hormone blood, Humans, Hyperglycemia complications, Hyperinsulinism complications, Leg, Fatty Acids, Nonesterified blood, Glucose metabolism, Hyperglycemia metabolism, Hyperinsulinism metabolism, Muscle, Skeletal metabolism, Viscera metabolism
- Abstract
The present study sought to determine whether elevated plasma free fatty acids (FFAs) alter the splanchnic and muscle glucose metabolism in women. To do so, FFAs were increased in seven women by an 8-h Intralipid/heparin (IL/hep) infusion, and the results were compared with those observed in nine women who were infused with glycerol alone. Glucose was clamped at approximately 8.3 mmol/l and insulin was increased to approximately 300 pmol/l to stimulate both muscle and hepatic glucose uptake. Insulin secretion was inhibited with somatostatin. Leg and splanchnic glucose metabolism were assessed using a combined catheter and tracer dilution approach. The glucose infusion rates required to maintain target plasma glucose concentrations were lower (P < 0.01) during IL/hep than glycerol infusion (30.8 +/- 2.6 vs. 65.0 +/- 7.9 micro mol. kg(-1). min(-1)). Whole-body glucose disappearance (37.0 +/- 2.2 vs. 70.9 +/- 8.7 micro mol. kg(-1). min(-1); P < 0.001) and leg glucose uptake (24.3 +/- 4.2 vs. 59.6 +/- 10.0 micro mol. kg fat-free mass of the leg(-1). min(-1); P < 0.02) were also lower, whereas splanchnic glucose production (8.2 +/- 0.8 vs. 4.3 +/- 0.7 micro mol. kg(-1). min(-1); P < 0.01) was higher during IL/hep than glycerol infusion. We conclude that in the presence of combined hyperinsulinemia and hyperglycemia, elevated FFAs impair glucose metabolism in women by inhibiting whole- body glucose disposal, muscle glucose uptake, and suppression of splanchnic glucose production.
- Published
- 2003
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- View/download PDF
4. Effect of enteral vs. parenteral glucose delivery on initial splanchnic glucose uptake in nondiabetic humans.
- Author
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Vella A, Shah P, Basu R, Basu A, Camilleri M, Schwenk WF, and Rizza RA
- Subjects
- Adult, C-Peptide blood, Duodenum, Female, Glucose biosynthesis, Hormones blood, Humans, Infusions, Parenteral, Injections, Injections, Intravenous, Insulin blood, Male, Reference Values, Uridine Diphosphate Glucose metabolism, Blood Glucose metabolism, Glucose administration & dosage, Splanchnic Circulation
- Abstract
To determine if enteral delivery of glucose influences splanchnic glucose metabolism, 10 subjects were studied when glucose was either infused into the duodenum at a rate of 22 micromol x kg(-1) x min(-1) and supplemental glucose given intravenously or when all glucose was infused intravenously while saline was infused intraduodenally. Hormone secretion was inhibited with somatostatin, and glucose (approximately 8.5 mmol/l) and insulin (approximately 450 pmol/l) were maintained at constant but elevated levels. Intravenously infused [6,6-(2)H(2)]glucose was used to trace the systemic appearance of intraduodenally infused [3-(3)H]glucose, whereas UDP-glucose flux (an index of hepatic glycogen synthesis) was measured using the acetaminophen glucuronide method. Despite differences in the route of glucose delivery, glucose production (3.5 +/- 1.0 vs. 3.3 +/- 1.0 micromol x kg(-1) x min(-1)) and glucose disappearance (78.9 +/- 5.7 vs. 85.0 +/- 7.2 micromol x kg(-1) x min(-1)) were comparable on intraduodenal and intravenous study days. Initial splanchnic glucose extraction (17.5 +/- 4.4 vs. 14.5 +/- 2.9%) and hepatic UDP-glucose flux (9.0 +/- 2.0 vs. 10.3 +/- 1.5 micromol x kg(-1) x min(-1)) also did not differ on the intraduodenal and intravenous study days. These data argue against the existence of an "enteric" factor that directly (i.e., independently of circulating hormone concentrations) enhances splanchnic glucose uptake or hepatic glycogen synthesis in nondiabetic humans.
- Published
- 2002
- Full Text
- View/download PDF
5. Effects of free fatty acids and glycerol on splanchnic glucose metabolism and insulin extraction in nondiabetic humans.
- Author
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Shah P, Vella A, Basu A, Basu R, Adkins A, Schwenk WF, Johnson CM, Nair KS, Jensen MD, and Rizza RA
- Subjects
- Adult, Blood Glucose analysis, C-Peptide blood, Fatty Acids, Nonesterified blood, Female, Glucose pharmacology, Glycerol blood, Humans, Insulin blood, Leg, Male, Muscle, Skeletal metabolism, Osmolar Concentration, Reference Values, Uridine Diphosphate Glucose metabolism, Fatty Acids, Nonesterified pharmacology, Glucose metabolism, Glycerol pharmacology, Insulin metabolism, Viscera metabolism
- Abstract
The present study sought to determine whether elevated plasma free fatty acids (FFAs) alter the ability of insulin and glucose to regulate splanchnic as well as muscle glucose metabolism. To do so, FFAs were increased in 10 subjects to approximately 1 mmol/l by an 8-h Intralipid/heparin (IL/Hep) infusion, whereas they fell to levels near the detection limit of the assay (<0.05 mmol/l) in 13 other subjects who were infused with glycerol alone at rates sufficient to either match (n = 5, low glycerol) or double (n = 8, high glycerol) the plasma glycerol concentrations observed during the IL/Hep infusion. Glucose was clamped at approximately 8.3 mmol/l, and insulin was increased to approximately 300 pmol/l to stimulate both muscle and hepatic glucose uptake. Insulin secretion was inhibited with somatostatin. Leg and splanchnic glucose metabolism were assessed using a combined catheter and tracer dilution approach. Leg glucose uptake (21.7 +/- 3.5 vs. 48.3 +/- 9.3 and 57.8 +/- 11.7 micromol x kg(-1) leg x min(-1)) was lower (P < 0.001) during IL/Hep than the low- or high-glycerol infusions, confirming that elevated FFAs caused insulin resistance in muscle. IL/Hep did not alter splanchnic glucose uptake or the contribution of the extracellular direct pathway to UDP-glucose flux. On the other hand, total UDP-glucose flux (13.2 +/- 1.7 and 12.5 +/- 1.0 vs. 8.1 +/- 0.5 micromol x kg(-1) x min(-1)) and flux via the indirect intracellular pathway (8.4 +/- 1.2 and 8.1 +/- 0.6 vs. 4.8 +/- 0.05 micromol x kg(-1) x min(-1)) were greater (P < 0.05) during both the IL/Hep and high-glycerol infusions than the low-glycerol infusion. In contrast, only IL/Hep increased (P < 0.05) splanchnic glucose production, indicating that elevated FFAs impaired the ability of the liver to autoregulate. Splanchnic insulin extraction, directly measured using the arterial and hepatic vein catheters, did not differ (67 +/- 3 vs. 71 +/- 5 vs. 69 +/- 1%) during IL/Hep and high- and low-glycerol infusions. We conclude that elevated FFAs exert multiple effects on glucose metabolism. They inhibit insulin- and glucose-induced stimulation of muscle glucose uptake and suppression of splanchnic glucose production. They increase the contribution of the indirect pathway to glycogen synthesis and impair hepatic autoregulation. On the other hand, they do not alter either splanchnic glucose uptake or splanchnic insulin extraction in nondiabetic humans.
- Published
- 2002
- Full Text
- View/download PDF
6. Higher insulin concentrations are required to suppress gluconeogenesis than glycogenolysis in nondiabetic humans.
- Author
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Adkins, Aron, Basu, Rita, Persson, Mai, Dicke, Betty, Shah, Pankaj, Vella, Adrian, Schwenk, W. Frederick, and Rizza, Robert
- Subjects
INSULIN ,GLUCOSE ,GLUCONEOGENESIS ,C-peptide - Abstract
To determine the mechanism(s) by which insulin inhibits endogenous glucose production (EGP) in nondiabetic humans, insulin was infused at rates of 0.25, 0.375, or 0.5 mU. kg(-1). min(-1) and glucose was clamped at approximately 5.5 mmol/l. EGP, gluconeogenesis, and uridine-diphosphoglucose (UDP)-glucose flux were measured using [3-(3)H]glucose, deuterated water, and the acetaminophen glucuronide methods, respectively. An increase in insulin from approximately 75 to approximately 100 to approximately 150 pmol/l ( approximately 12.5 to approximately 17 to approximately 25 microU/ml) resulted in progressive (ANOVA; P < 0.02) suppression of EGP (13.1 +/- 1.3 vs. 11.7 +/- 1.03 vs. 6.4 +/- 2.15 micromol x kg(-1) x min(-1)) that was entirely due to a progressive decrease (ANOVA; P < 0.05) in the contribution of glycogenolysis to EGP (4.7 +/- 1.7 vs. 3.4 +/- 1.2 vs. -2.1 +/- 1.3 micro mol x kg(-1) x min(-1)). In contrast, both the contribution of gluconeogenesis to EGP (8.4 +/- 1.0 vs. 8.3 +/- 1.1 vs. 8.5 +/- 1.3 micro mol x kg(-1) x min(-1)) and UDP-glucose flux (5.0 +/- 0.4 vs. 5.0 +/- 0.3 vs. 4.0 +/- 0.5 micro mol x kg(-1) x min(-1)) remained unchanged. The contribution of the direct (extracellular) pathway to UDP-glucose flux was minimal and constant during all insulin infusions. We conclude that higher insulin concentrations are required to suppress the contribution of gluconeogenesis of EGP than are required to suppress the contribution of glycogenolysis to EGP in healthy nondiabetic humans. Since suppression of glycogenolysis occurred without a decrease in UDP-glucose flux, this implies that insulin inhibits EGP, at least in part, by directing glucose-6-phosphate into glycogen rather than through the glucose-6-phosphatase pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
7. Enteral and infusion of glucose at rates approximating EGP enhances glucose disposal but does not cause hypoglycemia.
- Author
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Zangeneh, Farhad, Basu, Rita, Shah, Pankaj, Arora, Puneet, Camilleri, Michael, and Rizza, Robert A.
- Subjects
GLUCOSE ,PHYSIOLOGICAL control systems ,HYPOGLYCEMIA - Abstract
Portal infusion of glucose at rates approximating endogenous glucose production (EGP) causes paradoxical hypoglycemia in wild-type but not GLUT2 null mice, implying activation of a specific portal glucose sensor. To determine whether this occurs in humans, glucose containing [3-³H]glucose was infused intraduodenally at rates of 3.1 mg·kg[sup -1] · min[sup -1] (n = 5), 1.55 mg·kg[sup -1]·min[sup -1] (n = 9), or 0/0.1 mg·kg[sup -1]·min[sup -1] (n = 9) for 7 h in healthy nondiabetic subjects. [6,6-²H[sub 2]]glucose was infused intravenously to enable simultaneous measurement of EGP, glucose disappearance, and the rate of appearance of the intraduodenally infused glucose. Plasma glucose concentrations fell (P < 0.01) from 90 ± 1 to 84 ± 2 mg/dl during the 0/0.1 mg·kg[sup -1]·min[sup -1] id infusions but increased (P < 0.001) to 104 ± 5 and 107 ± 3 mg/dl, respectively, during the 1.55 and 3.1 mg·kg[sup -1]·min[sup -1] id infusions. In contrast, insulin increased (P < 0.05) during the 1.55 and 3.0 mg·kg[sup -1]·min[sup -1] infusions, reaching a peak of 10 ± 2 and 18 ± 5 µU/ml, respectively, by 2 h. Insulin concentrations then fell back to concentrations that no longer differed by study end (7 ± 1 vs. 8 ± 1 µU/ml). This resulted in comparable suppression of EGP by study end (0.84 ± 0.2 and 0.63 ± 0.1 mg·kg[sup -1]. min[sup -1]). Glucose disappearance was higher (P < 0.01) during the final hour of the 3.1 than 1.55 mg·kg[sup -1]·min[sup -1] id infusion (4.47 ± 0.2 vs. 2.6 ± 0.1 mg·kg[sup -1]·min[sup -1]), likely because of the slightly, but not significantly, higher glucose and insulin concentrations. We conclude that, in contrast to mice, selective portal glucose delivery at rates approximating EGP does not cause hypoglycemia in humans. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
8. Type 2 diabetes impairs splanchnic uptake of glucose but does not alter intestinal glucose absorption during enteral glucose feeding: additional evidence for a defect in hepatic glucokinase activity.
- Author
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Basu, Ananda, Basu, Rita, Shah, Pankaj, Vella, Adrian, Johnson, C. Michael, Jensen, Michael, Nair, K. Sreekumaran, Schwenk, W. Frederick, Rizza, Robert A., Basu, A, Basu, R, Shah, P, Vella, A, Johnson, C M, Jensen, M, Nair, K S, Schwenk, W F, and Rizza, R A
- Subjects
TYPE 2 diabetes ,GLUCOSE ,HYPERGLYCEMIA - Abstract
We have previously reported that splanchnic glucose uptake, hepatic glycogen synthesis, and hepatic glucokinase activity are decreased in people with type 2 diabetes during intravenous glucose infusion. To determine whether these defects are also present during more physiological enteral glucose administration, we studied 11 diabetic and 14 nondiabetic volunteers using a combined organ catheterization-tracer infusion technique. Glucose was infused into the duodenum at a rate of 22 micromol. kg(-1). min(-1) while supplemental glucose was given intravenously to clamp glucose at approximately 10 mmol/l in both groups. Endogenous hormone secretion was inhibited with somatostatin, and insulin was infused to maintain plasma concentrations at approximately 300 pmol/l (i.e., twofold higher than our previous experiments). Total body glucose disappearance, splanchnic, and leg glucose extractions were markedly lower (P < 0.01) in the diabetic subjects than in the nondiabetic subjects. UDP-glucose flux, a measure of glycogen synthesis, was approximately 35% lower (P < 0.02) in the diabetic subjects than in the nondiabetic subjects. This was entirely accounted for by a decrease (P < 0.01) in the contribution of extracellular glucose because the contribution of the indirect pathway to hepatic glycogen synthesis was similar between groups. Neither endogenous and splanchnic glucose productions nor rates of appearance of the intraduodenally infused glucose in the portal vein differed between groups. In summary, both muscle and splanchnic glucose uptake are impaired in type 2 diabetes during enteral glucose administration. The defect in splanchnic glucose uptake appears to be due to decreased uptake of extracellular glucose, implying decreased glucokinase activity. Thus, abnormal hepatic and muscle (but not gut) glucose metabolism are likely to contribute to postprandial hyperglycemia in people with type 2 diabetes. [ABSTRACT FROM AUTHOR]
- Published
- 2001
- Full Text
- View/download PDF
9. Effect of glucagon-like peptide 1(7-36) amide on glucose effectiveness and insulin action in people with type 2 diabetes.
- Author
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Vella, Adrian, Shah, Pankaj, Basu, Rita, Basu, Ananda, Holst, Jens J., Rizza, Robert A., Vella, A, Shah, P, Basu, R, Basu, A, Holst, J J, and Rizza, R A
- Subjects
- *
GLUCAGON-like peptide 1 , *GLUCOSE , *INSULIN , *TYPE 2 diabetes , *PHYSIOLOGY - Abstract
Although it is well established that glucagon-like peptide 1(7-36) amide (GLP-1) is a potent stimulator of insulin secretion, its effects on insulin action and glucose effectiveness are less clear. To determine whether GLP-1 increases insulin action and glucose effectiveness, subjects with type 2 diabetes were studied on two occasions. Insulin was infused during the night on both occasions to ensure that baseline glucose concentrations were comparable. On the morning of study, either GLP-1 (1.2 pmol x kg(-1) x min(-1)) or saline were infused along with somatostatin and replacement amounts of glucagon. Glucose also was infused in a pattern mimicking that typically observed after a carbohydrate meal. Insulin concentrations were either kept constant at basal levels (n = 6) or varied so as to create a prandial insulin profile (n = 6). The increase in glucose concentration was virtually identical on the GLP-1 and saline study days during both the basal (1.21 +/- 0.15 vs. 1.32 +/- 0.19 mol/l per 6 h) and prandial (0.56 +/- 0.14 vs. 0.56 +/- 0.10 mol/l per 6 h) insulin infusions. During both the basal and prandial insulin infusions, glucose disappearance promptly increased after initiation of the glucose infusion to rates that did not differ on the GLP-1 and saline study days. Suppression of endogenous glucose production also was comparable on the GLP-1 and saline study days during both the basal (-2.7 +/- 0.3 vs. -3.1 +/- 0.2 micromol/kg) and prandial (-3.1 +/- 0.4 vs. -3.0 +/- 0.6 pmol/kg) insulin infusions. We conclude that when insulin and glucagon concentrations are matched, GLP-1 has negligible effects on either insulin action or glucose effectiveness in people with type 2 diabetes. These data strongly support the concept that GLP-1 improves glycemic control in people with type 2 diabetes by increasing insulin secretion, by inhibiting glucagon secretion, and by delaying gastric emptying rather than by altering extrapancreatic glucose metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2000
- Full Text
- View/download PDF
10. UIAA Medical Commission Recommendations for Mountaineers, Hillwalkers, Trekkers, and Rock and Ice Climbers with Diabetes.
- Author
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Hillebrandt, David, Gurtoo, Anil, Kupper, Thomas, Richards, Paul, Schöffl, Volker, Shah, Pankaj, van der Spek, Rianne, Wallis, Nikki, and Milledge, Jim
- Subjects
- *
MOUNTAINEERS , *MOUNTAINEERING , *HIKERS , *DIABETES , *PEOPLE with diabetes - Abstract
Hillebrandt, David, Anil Gurtoo, Thomas Kupper, Paul Richards, Volker Schöffl, Pankaj Shah, Rianne van der Spek, Nikki Wallis, and Jim Milledge. UIAA Medical Commission recommendations for mountaineers, hillwalkers, trekkers, and rock and ice climbers with diabetes. High Alt Med Biol. 24: 110–126.—The object of this advice article is not only to give the diabetic mountaineer general guidance but also to inform his or her medical team of practical aspects of care that may not be standard for nonmountaineers. The guidelines are produced in seven sections. The first is an introduction to the guidelines, and the second is an introduction to this medical problem and is designed to be read and understood by diabetic patients and their companions. The third section is for use in an emergency in mountains. The fourth is for rock, ice, and competition climbers operating in a less remote environment. These initial sections are deliberately written in simple language. The fifth and sixth sections are written for clinicians and those with skills to read more technical information, and the seventh looks at modern technology and its pros and cons in diabetes management in a remote area. Sections One and Two could be laminated and carried when in the mountains, giving practical advice. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
11. Pioglitazone and Metformin Differentially Modulate Free Fatty Acid (FFA) Induced Insulin Resistance in Type 2 Diabetes.
- Author
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Basu, Rita, Basu, Ananda, Norby, Barbara, Chandramouli, Visvanathan, Shah, Pankaj, Landau, Bernard R., and Rizza, Robert A.
- Subjects
HYPOGLYCEMIC agents ,FATTY acids ,INSULIN resistance ,TYPE 2 diabetes ,INSULIN ,GLUCOSE - Abstract
To determine if, and if so, the mechanism(s) by which Pioglitazone and Metformin alter FFA induced insulin resistance, type 2 diabetic subjects were infused with either Intralipid/heparin (IL) in amounts sufficient to maintain FFA in the range observed in poorly controlled diabetes (∼0.5 mmol/l) or matching amounts of glycerol in random order prior to and following four months of treatment with either Pioglitazone (n=16) or Metformin (n=15). Glucose was clamped at ∼5.3 mmol/l; endogenous hormone secretion inhibited with somatostatin; and insulin concentrations raised to ∼190 pmol/l by a 0.6 mU/kg/min insulin infusion. Prior to treatment, FFA impaired substantially (p<0.01) insulin induced stimulation of glucose uptake but minimally altered suppression of endogenous glucose production (EGP). Pioglitazone and Metformin both increased net insulin action (glucose infusion rate required to maintain euglycemia) in the presence (p<0.001) of elevated FFA but only Pioglitazone did so in the absence (p<0.01) of elevated FFA. Pioglitazone increased (p<0.01) glucose uptake and improved suppression (p<0.05) of EGP during IL to rates observed during glycerol infusion prior to treatment. However despite treatment with Pioglitazone, FFA still (i.e. compared to the post-treatment glycerol infusion) impaired the ability of insulin to stimulate (p<0.0001) glucose uptake and suppress EGP (p<0.01). In contrast when compared to pretreatment, whereas Metformin also increased (p<0.05) glucose uptake during IL, it did not enhance suppression of EGP either in the presence or absence of elevated FFA. On the other hand, elevated FFA (i.e. compared to the post-treatment glycerol infusion) had no effect on glucose uptake following treatment with Metformin. Thus, both Pioglitazone and Metformin improve net insulin action in the presence of elevated FFA. However, Metformin but not Pioglitazone prevents the inhibitory effects of FFA on insulin induced stimulation of glucose uptake. In contrast, ,while not totally preventing the stimulatory effects of elevated FFA on hepatic glucose release, Pioglitazone but not Metformin enhances insulin induced suppression of EGP. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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