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Start Over Search Limiters Full Text Topic hydrolases
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1. Autolytic enzyme system of Clostridium botulinum. II. Mode of action of autolytic enzymes in Clostridium botulinum type A.

Author

Takumi K, Kawata T, and Hisatsune K

Subjects
Amino Acids analysis, Autolysis, Chromatography, DEAE-Cellulose, Chromatography, Gel, Chromatography, Ion Exchange, Chromatography, Paper, Electrophoresis, Glycosides, Paper, Peptides isolation & purification, Polysaccharides isolation & purification, Clostridium botulinum enzymology, Glycoside Hydrolases, Hexosamines, Hydrolases analysis, Muramidase analysis
Published
1971
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2. Saccharification of used paper with different cellulases.

Author

van Wyk, J. P. H., Mogale, M. A., and Seseng, T. A.

Subjects
CELLULASE, HYDROLASES, PENICILLIUM, TRICHODERMA, ASPERGILLUS, BIOCHEMISTRY
Abstract

Various used paper materials have been exposed to the action of cellulases from Penicillium funiculosum, Trichoderma reesei, Trichoderma viride and Aspergillus niger. A 2 h incubation period showed cellulase from T. viride the most active except for office paper that was maximally degraded by A. niger cellulase. Cellulase mixtures increased saccharification while sequential treatment with cellulases from T. reesei and P. funiculosum increased biodegradation at values between 15% and 190%. The maximum increase of saccharification (190%) was obtained when T. reesei cellulase initiated the sequential treatment of newspaper relative to the sole action of P. funiculosum cellulase on this non-pretreated and pretreated material. [ABSTRACT FROM AUTHOR]

Published
2000
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3. Autolytic enzyme system of Clostridium botulinum. II. Mode of action of autolytic enzymes in Clostridium botulinum type A

Author

Tomio Kawata, Kenji Takumi, and Kazuhito Hisatsune

Subjects
Electrophoresis, Paper, Glycoside Hydrolases, Chromatography, Paper, Hydrolases, Clostridium botulinum type A, medicine.disease_cause, Chromatography, DEAE-Cellulose, Microbiology, Enzyme system, Polysaccharides, medicine, Clostridium botulinum, Glycosides, Amino Acids, Mode of action, chemistry.chemical_classification, Chemistry, Hexosamines, General Medicine, Chromatography, Ion Exchange, Enzyme, Chromatography, Gel, Muramidase, Autolysis, Peptides
Published
1971

4. Comparison of different fungal enzymes for bleaching high-quality paper pulps

Author

José F. Colom, Ángel T. Martínez, Michèle Asther, Marta Pérez-Boada, María Jesús Martínez, Teresa Vidal, Cécile Sigoillot, Susana Camarero, Eric Record, Jean-Claude Sigoillot, Marcel Asther, Unité mixte de recherche de biotechnologie des champignons filamenteux, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Université de Provence - Aix-Marseille 1, and Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)

Subjects
0106 biological sciences, Hydrolases, [SDV]Life Sciences [q-bio], RECOMBINANT ENZYME, Pleurotus, 01 natural sciences, Applied Microbiology and Biotechnology, Lignin, chemistry.chemical_compound, Feruloyl esterase, [SDV.IDA]Life Sciences [q-bio]/Food engineering, PAPER PULP, OXIDOREDUCTASE, MEDIATOR, Food science, BIOTECHNOLOGIE, 0303 health sciences, biology, Chemistry, Pulp (paper), General Medicine, Hydrogen-Ion Concentration, Recombinant Proteins, Biochemistry, Phanerochaete, Aspergillus niger, Oxidoreductases, Biotechnology, Paper, Bioengineering, engineering.material, 03 medical and health sciences, Industrial Microbiology, stomatognathic system, 010608 biotechnology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Pleurotus eryngii, 030304 developmental biology, Laccase, Pycnoporus cinnabarinus, Triazoles, biology.organism_classification, BIOBLEACHING, stomatognathic diseases, FLAX, engineering
Abstract

International audience; Wild and recombinant hydrolases and oxidoreductases with a potential interest for environmentally sound bleaching of high-quality paper pulp (from flax) were incorporated into a totally chlorine free (TCF) sequence that also included a peroxide stage. The ability of feruloyl esterase (from Aspergillus niger) and Mn2+-oxidizing peroxidases (from Phanerochaete chrysosporium and Pleurotus eryngii) to decrease the final lignin content of flax pulp was shown. Laccase from Pycnoporus cinnabarinus (without mediator) also caused a slight improvement of pulp brightness that was increased in the presence of aryl-alcohol oxidase. However, the best results were obtained when the laccase treatment was performed in the presence of a mediator, 1-hydroxybenzotriazol (HBT), enabling strong delignification of pulps. The enzymatic removal of lignin resulted in high-final brightness values that are difficult to attain by chemical bleaching of this type of pulp. A partial inactivation of laccase by HBT was observed but this negative effect was strongly reduced in the presence of pulp. The good results obtained with the same laccase expressed in A. niger at bioreactor scale, revealed the feasibility of using recombinant laccase for bleaching high-quality non-wood pulps in the presence of a mediator.