1. Characterization and Neuroprotection Potential of Seleno-Polymannuronate.
- Author
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Bi, Decheng, Li, Xiaofan, Li, Tong, Li, Xiuting, Lin, Zhijian, Yao, Lijun, Li, Hui, Xu, Hong, Hu, Zhangli, Zhang, Zhenqing, Liu, Qiong, and Xu, Xu
- Subjects
SELENOPROTEINS ,CYTOCHROME c ,SUBSTITUTION reactions ,MEMBRANE potential ,MOLECULAR weights ,PROTEIN expression - Abstract
Seleno-polymannuronate (Se-PM) was prepared from alginate-derived polymannuronate (PM) through a sulfation followed by a selenylation replacement reaction. The organic selenium content of Se-PM was 437.25 μg/g and its average molecular weight was 2.36 kDa. The neuroprotection effect of Se-PM and corresponding molecular mechanisms were investigated. Our results showed that, comparing to both sulfated PM (S-PM) and PM, Se-PM remarkably inhibited the aggregation of Aβ
1–42 oligomer in vitro and significantly reduced the APP and BACE1 protein expression in N2a-sw cells, highlighting the critical function of the selenium presented in Se-PM. Moreover, Se-PM decreased the expression of cytochrome c and the ratio of Bax to Bcl-2, and enhanced the mitochondrial membrane potential in N2a-sw cells. These results suggested that Se-PM treatment can markedly inhibit N2a-sw cell apoptosis and promote N2a-sw cell survival and that Se-PM might be a potential therapeutic agent for the prevention of neurodegeneration owing to its remarkable neuroprotection effect. [ABSTRACT FROM AUTHOR]- Published
- 2020
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