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2 results on '"Aasted, B."'

1. Analyses of monoclonal antibodies reacting with porcine wCD6 Results from the Second International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Petrinec, N., and Saalmüller, A.

Subjects
Antigens, Differentiation, T-Lymphocyte, General Veterinary, Swine, T-cells, Immunology, Antibodies, Monoclonal, Flow Cytometry, Antigen-Antibody Reactions, Porcine wCD6, Antigens, CD, T-Lymphocyte Subsets, Monoclonal antibodies, porcine wCD6, Animals
Abstract

Among the 57 monoclonal antibodies analyzed within the T-cell group of the Second International Swine CD Workshop, one mAb fell within cluster T14a that included the CD6 standard a38b2 (No. 175). The new mAb MIL8 (No. 082) and a38b2 both precipitated from activated T-cells a 150 kDa monomeric protein. Staining patterns on the various cell types were similar. There was no inhibition of binding of either mAb to peripheral blood T-cells with the opposite mAb. The new mAb, MIL8, reacts with a separate epitope on porcine wCD6.

Published
1998

2. Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): Cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

Author

Petersen, C.B., Nygård, A.-B., Viuff, B., Fredholm, M., Aasted, B., and Salomonsen, J.

Subjects
*SWINE, *MONOCLONAL antibodies, *PHOSPHATASES, *PHOSPHODIESTERASES
Abstract

Abstract: Swine workshop cluster 9 (SWC9) antibody identifying a porcine epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids. [Copyright &y& Elsevier]

Published
2007
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