1. Innervation of endometrium and myometrium in women with painful adenomyosis and uterine fibroids
- Author
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Zhang, Xinmei, Lu, Bangchun, Huang, Xiufeng, Xu, Hong, Zhou, Caiyun, and Lin, Jun
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ENDOMETRIAL diseases , *MYOMETRIUM , *DISEASES in women , *ENDOMETRIOSIS , *UTERINE fibroids , *RETROSPECTIVE studies , *IMMUNOHISTOCHEMISTRY , *LABORATORY mice , *NERVE fibers , *DISEASES , *PATIENTS , *COMPARATIVE studies , *ENDOMETRIUM , *ESTERASES , *IMMUNITY , *RESEARCH methodology , *MEDICAL cooperation , *NERVE tissue proteins , *NEURONS , *PELVIC pain , *RESEARCH , *EVALUATION research , *INNERVATION - Abstract
Objective: To determine whether nerve fibers can be detected in the endometrium and myometrium in women with painful uterine fibroids and adenomyosis.Design: A retrospective immunohistochemical study.Setting: An academic training hospital.Patient(s): Thirty-seven women with uterine fibroids and 29 women with adenomyosis.Intervention(s): Histologic sections of contiguous endometrial and myometrial tissues were stained immunohistochemically using the highly specific polyclonal rabbit antiprotein gene product 9.5 (PGP9.5) and monoclonal mouse antineurofilament protein (NF).Main Outcome Measure(s): Results were determined through immunohistochemical staining using PGP9.5 and NF.Result(s): We detected PGP9.5-immunoactive nerve fibers in the functional layer of the endometrium in women with pain but not in women without pain. PGP9.5-immunoactive nerve fiber density in the basal layer of the endometrium or myometrium significantly increased in women with pain, however, PGP9.5-immunoactive nerve fiber density had no statistical differences between women with adenomyosis and uterine fibroids. We identified NF-immunoactive nerve fibers in the basal layer of the endometrium and myometrium in women with adenomyosis and uterine fibroids, but found no significant differences.Conclusion(s): These results suggest that PGP9.5-immunoactive nerve fibers appearing in the endometrium and myometrium of women with painful adenomyosis and uterine fibroids may play a role in pain generation in these two disorders. [ABSTRACT FROM AUTHOR]- Published
- 2010
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