12 results on '"Cheng, Lirui"'
Search Results
2. Transcriptome Analysis and Genome-Wide Gene Family Identification Enhance Insights into Bacterial Wilt Resistance in Tobacco.
- Author
-
Liu, Zhengwen, Xiao, Zhiliang, Geng, Ruimei, Ren, Min, Wu, Xiuming, Xie, He, Bai, Ge, Zhang, Huifen, Liu, Dan, Jiang, Caihong, Cheng, Lirui, and Yang, Aiguo
- Subjects
BACTERIAL wilt diseases ,DRUG resistance in bacteria ,GENE families ,HEAT shock proteins ,GENE expression ,TRANSCRIPTOMES - Abstract
Bacterial wilt, caused by the Ralstonia solanacearum species complex, is one of the most damaging bacterial diseases in tobacco and other Solanaceae crops. In this study, we conducted an analysis and comparison of transcriptome landscape changes in seedling roots of three tobacco BC
4 F5 lines, C244, C010, and C035, with different resistance to bacterial wilt at 3, 9, 24, and 48 h after R. solanacearum infection. A number of biological processes were highlighted for their differential enrichment between C244, C010, and C035, especially those associated with cell wall development, protein quality control, and stress response. Hence, we performed a genome-wide identification of seven cell wall development-related gene families and six heat shock protein (Hsp) families and proposed that genes induced by R. solanacearum and showing distinct expression patterns in C244, C010, and C035 could serve as a potential gene resource for enhancing bacterial wilt resistance. Additionally, a comparative transcriptome analysis of R. solanacearum-inoculated root samples from C244 and C035, as well as C010 and C035, resulted in the identification of a further 33 candidate genes, of which Nitab4.5_0007488g0040, a member of the pathogenesis-related protein 1 (PR-1) family, was found to positively regulate bacterial wilt resistance, supported by real-time quantitative PCR (qRT-PCR) and virus-induced gene silencing (VIGS) assays. Our results contribute to a better understanding of molecular mechanisms underlying bacterial wilt resistance and provide novel alternative genes for resistance improvement. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
3. Genetic diversity and fingerprinting of 33 standard flue-cured tobacco varieties for use in distinctness, uniformity, and stability testing
- Author
-
He, Binbin, Geng, Ruimei, Cheng, Lirui, Yang, Xianbin, Ge, Hongmei, and Ren, Min
- Published
- 2020
- Full Text
- View/download PDF
4. Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (sld) Mutant from Tetraploid Common Tobacco (Nicotiana tabacum L.).
- Author
-
Wang, Shaomei, Wu, Xinru, Guo, Yongfeng, Wang, Dawei, Cheng, Lirui, Wang, Yuanying, Yang, Aiguo, and Liu, Guanshan
- Subjects
GENE mapping ,MICROSATELLITE repeats ,PHENOTYPES ,REGULATOR genes ,LEAF morphology ,DEVELOPMENTAL biology ,RECESSIVE genes ,TOBACCO - Abstract
Leaf morphology and plant height are two agronomic traits closely related to tobacco (Nicotiana tabacum L.) yield and quality. The study of leaf morphology and plant stature mutants will greatly contribute to the fields of plant architecture breeding and developmental biology. Here, we report the characterization of a spiral leaf and dwarf (sld) mutant identified from an ethylmethane sulfonate (EMS)-induced common tobacco population. The sld mutant displayed the phenotype of wrinkled, spiral, and miniature leaves, with the growth point as the central axis and plant dwarfing with shortened internodes. The inheritance pattern of the sld mutant phenotype was manipulated by a recessive nuclear monogene, which was linked to six tobacco simple sequence repeat (SSR) markers from linkage group 5 via gene mapping. Utilizing an F
2 population, the sld mutant gene the sld mutant gene was located between the co-segregated markers PT51778, PT54913, and the marker PT61414, with an equal genetic distance of 0.16 cM. Taking advantage of a BC1 F1 population, the markers PT51778, PT54913, the sld gene, and the marker PT61414 demonstrated co-segregation, located between the markers PT40040 and PT60933, respectively, with a genetic distance of 1.37 cM and 6.32 cM, respectively. These findings will be helpful in cloning the sld gene and in the further characterization of the regulatory genes controlling the spiral and dwarfing phenotypes in tobacco. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
5. Identification of a major QTL affecting resistance to brown spot in tobacco (Nicotiana tabacum L.) via linkage and association mapping methods
- Author
-
Sun, Mingming, Cheng, Lirui, Jiang, Caihong, Zhu, Chengguang, Ren, Min, Zhang, Yusheng, Zhang, Yu, Liu, Dan, Zhao, Qiang, Geng, Ruimei, Hu, Xiaoli, Yang, Aiguo, and Wang, Yuanying
- Published
- 2018
- Full Text
- View/download PDF
6. Naringenin confers defence against Phytophthora nicotianae through antimicrobial activity and induction of pathogen resistance in tobacco.
- Author
-
Sun, Mingming, Li, Lei, Wang, Chengdong, Wang, Luanming, Lu, Di, Shen, Danyu, Wang, Jie, Jiang, Caihong, Cheng, Lirui, Pan, Xuhao, Yang, Aiguo, Wang, Yuanying, Zhu, Xiaowei, Li, Bin, Li, Yiting, and Zhang, Feng
- Subjects
PHYTOPHTHORA nicotianae ,PHYTOPHTHORA ,TOBACCO smoke ,RALSTONIA solanacearum ,NARINGENIN ,ANTI-infective agents ,TOBACCO ,CHALCONE synthase - Abstract
Tobacco black shank caused by Phytophthora nicotianae is a serious disease in tobacco cultivation. We found that naringenin is a key factor that causes different sensitivity to P. nicotianae between resistant and susceptible tobacco. The level of basal flavonoids in resistant tobacco was distinct from that in susceptible tobacco. Of all flavonoids with different content, naringenin showed the best antimicrobial activity against mycelial growth and sporangia production of P. nicotianae in vitro. However, naringenin showed very low or no antimicrobial activity to other plant pathogens. We found that naringenin induced not only the accumulation of reactive oxygen species, but also the expression of salicylic acid biosynthesis‐related genes. Naringenin induced the expression of the basal pathogen resistance gene PR1 and the SAR8.2 gene that contributes to plant resistance to P. nicotianae. We then interfered with the expression of the chalcone synthase (NtCHS) gene, the key gene of the naringenin synthesis pathway, to inhibit naringenin biosynthesis. NtCHS‐RNAi rendered tobacco highly sensitive to P. nicotianae, but there was no change in susceptibility to another plant pathogen, Ralstonia solanacearum. Finally, exogenous application of naringenin on susceptible tobacco enhanced resistance to P. nicotianae and naringenin was very stable in this environment. Our findings revealed that naringenin plays a core role in the defence against P. nicotianae and expanded the possibilities for the application of plant secondary metabolites in the control of P. nicotianae. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
7. Transcriptome analysis of two cultivars of tobacco in response to Cucumber mosaic virus infection
- Author
-
Caihong Jiang, Qiang Zhao, Yazeng Cheng, Cheng Lirui, Yang Aiguo, Dandan Li, Dan Liu, and Yuanying Wang
- Subjects
0301 basic medicine ,lcsh:Medicine ,Biology ,Plant disease resistance ,Cucumovirus ,Article ,Virus ,Microbiology ,Transcriptome ,Cucumber mosaic virus ,03 medical and health sciences ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Plant virus ,Tobacco ,Cultivar ,lcsh:Science ,Disease Resistance ,Plant Diseases ,Plant Proteins ,Molecular breeding ,Multidisciplinary ,Gene Expression Profiling ,fungi ,lcsh:R ,food and beverages ,Gene expression profiling ,030104 developmental biology ,Host-Pathogen Interactions ,lcsh:Q ,030217 neurology & neurosurgery - Abstract
Cucumber mosaic virus (CMV) is among the most important plant virus infections, inducing a variety of disease symptoms. However, the molecular mechanisms underlying plant responses to CMV infection remain poorly understood. In this study, we performed RNA sequencing analysis of tolerant (Taiyan8) and susceptible (NC82) tobacco cultivars on CMV-infected plants, using mock-inoculated plants as a control. The propagation of CMV in inoculated leaves did not show obvious difference between two cultivars, whereas virus accumulation in systemic leaves of Taiyan8 was smaller than those of NC82 at the same time point. We observed 765 and 1,011 differentially expressed genes (DEGs) in Taiyan8 and NC82, respectively, in CMV-inoculated leaves. DEGs related to reactive oxygen species, salicylic acid signal transduction, and plant–pathogen interaction were upregulated or downregulated in Taiyan8, which indicates that defense response pathways to CMV were activated in the tolerant cultivar. In addition, we identified several DEGs related to disease defense and stress resistance showing opposing expression patterns in the two cultivars. Our comparative transcriptome analysis will improve our understanding of the mechanisms of CMV tolerance in plants, and will be of great importance in the molecular breeding of CMV- tolerant genotypes.
- Published
- 2019
8. Comparative transcriptome analysis reveals resistant and susceptible genes in tobacco cultivars in response to infection by Phytophthora nicotianae
- Author
-
Mingming Sun, Caihong Jiang, Feng Quanfu, Ying Sun, Cheng Lirui, Yuanying Wang, Zipeng Jiang, Yang Aiguo, Ren Min, Meng He, Dan Liu, Wenlong Yu, Guangdi Yuan, and Liu Yutong
- Subjects
0106 biological sciences ,0301 basic medicine ,Phytophthora ,Plant genetics ,Science ,01 natural sciences ,Plant Roots ,Article ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,Plant immunity ,Tobacco ,Protein kinase A ,Gene ,Transcription factor ,Disease Resistance ,Plant Diseases ,Plant Proteins ,Genetics ,Multidisciplinary ,biology ,Kinase ,Sequence Analysis, RNA ,Gene Expression Profiling ,Callose ,Phytophthora nicotianae ,biology.organism_classification ,WRKY protein domain ,Plant Breeding ,030104 developmental biology ,Gene Ontology ,chemistry ,Medicine ,010606 plant biology & botany - Abstract
Phytophthora nicotianae is highly pathogenic to Solanaceous crops and is a major problem in tobacco production. The tobacco cultivar Beihart1000-1 (BH) is resistant, whereas the Xiaohuangjin 1025 (XHJ) cultivar is susceptible to infection. Here, BH and XHJ were used as models to identify resistant and susceptible genes using RNA sequencing (RNA-seq). Roots were sampled at 0, 6, 12, 24, and 60 h post infection. In total, 23,753 and 25,187 differentially expressed genes (DEGs) were identified in BH and XHJ, respectively. By mapping upregulated DEGs to the KEGG database, changes of the rich factor of “plant pathogen interaction pathway” were corresponded to the infection process. Of all the DEGs in this pathway, 38 were specifically regulated in BH. These genes included 11 disease-resistance proteins, 3 pathogenesis-related proteins, 4 RLP/RLKs, 2 CNGCs, 7 calcium-dependent protein kinases, 4 calcium-binding proteins, 1 mitogen-activated protein kinase kinase, 1 protein EDS1L, 2 WRKY transcription factors, 1 mannosyltransferase, and 1 calmodulin-like protein. By combining the analysis of reported susceptible (S) gene homologs and DEGs in XHJ, 9 S gene homologs were identified, which included 1 calmodulin-binding transcription activator, 1 cyclic nucleotide-gated ion channel, 1 protein trichome birefringence-like protein, 1 plant UBX domain-containing protein, 1 ADP-ribosylation factor GTPase-activating protein, 2 callose synthases, and 2 cellulose synthase A catalytic subunits. qRT-PCR was used to validate the RNA-seq data. The comprehensive transcriptome dataset described here, including candidate resistant and susceptible genes, will provide a valuable resource for breeding tobacco plants resistant to P. nicotianae infections.
- Published
- 2021
9. Identification of QTLs Associated With Agronomic Traits in Tobacco via a Biparental Population and an Eight-Way MAGIC Population.
- Author
-
Liu, Yutong, Yuan, Guangdi, Si, Huan, Sun, Ying, Jiang, Zipeng, Liu, Dan, Jiang, Caihong, Pan, Xuhao, Yang, Jun, Luo, Zhaopeng, Zhang, Jianfeng, Ren, Min, Pan, Yi, Sun, Kefan, Meng, He, Wen, Liuying, Xiao, Zhiliang, Feng, Quanfu, Yang, Aiguo, and Cheng, Lirui
- Abstract
Agronomic traits such as plant height (PH), leaf number (LN), leaf length (LL), and leaf width (LW), which are closely related to yield and quality, are important in tobacco (Nicotiana tabacum L.). To identify quantitative trait loci (QTLs) associated with agronomic traits in tobacco, 209 recombinant inbred lines (RILs) and 537 multiparent advanced generation intercross (MAGIC) lines were developed. The biparental RIL and MAGIC lines were genotyped using a 430 K single-nucleotide polymorphism (SNP) chip assay, and their agronomic traits were repeatedly evaluated under different conditions. A total of 43 QTLs associated with agronomic traits were identified through a combination of linkage mapping (LM) and association mapping (AM) methods. Among these 43 QTLs, three major QTLs, namely qPH13-3, qPH17-1 , and qLW20-1 , were repeatedly identified by the use of various genetically diverse populations across different environments. The candidate genes for these major QTLs were subsequently predicted. Validation and utilization of the major QTL qLW20-1 for the improvement of LW in tobacco were investigated. These results could be applied to molecular marker-assisted selection (MAS) for breeding important agronomic traits in tobacco. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
10. Development and application of a molecular marker for TMV resistance based on N gene in tobacco (Nicotiana tabacum).
- Author
-
Zhang Yu, Wen Liuying, Yang Aiguo, Luo Chenggang, Cheng Lirui, Jiang Caihong, Chang Aixia, Li Wei, Zhang Jing, Xiao Zhixin, and Wang Yuanying
- Abstract
Tobacco mosaic virus (TMV) caused serious loss in yield and quality of tobacco every year. It is a long-term goal to improve the tobacco resistance against TMV by tobacco breeding. N gene was the firstly reported TMV-resistant gene, which showed resistance against all Tobamoviruses except the Ob stain and belonged to the toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat class of plant resistance (R) genes. At present, N gene had already been widely used in tobacco conventional breeding, but there is rare available molecular maker used in marker-assisted selection of TMV resistance. In this study, we designed a pair of primers that specific amplify N gene fragment based on the sequence of N gene intron III, named N-marker. Then, we identified TMV resistance by two selecting methods, PCR with N-marker and inoculated with the TMV-C strain. Results from the two method showed that (1) 13 varieties among 67 tobacco varieties displayed hypersensitive reaction when inoculated with the TMV-C strain, also contained N gene fragments screened by PCR with N-marker; (2) 105 strains of 200 BC1 strains showed resistance against TMV when inoculated with TMV-C strain, meanwhile, 103 of the 105 strains contained N gene fragment verified by PCR with N-marker. Therefore, the N-marker is reliable for high throughput screening of germplasm resources and tobacco breeding materials in selection of N-mediated TMV resistance. Our study not only developed a molecular marker for tobacco breeding, but also identified new germplasm resources that are resistant to TMV. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
11. Genome-wide identification of the TIFY gene family in tobacco and expression analysis in response to Ralstonia solanacearum infection.
- Author
-
Zhang, Huifen, Liu, Zhengwen, Geng, Ruimei, Ren, Min, Cheng, Lirui, Liu, Dan, Jiang, Caihong, Wen, Liuying, Xiao, Zhiliang, and Yang, Aiguo
- Subjects
- *
BACTERIAL wilt diseases , *GENE families , *RALSTONIA solanacearum , *TOBACCO analysis , *DRUG resistance in bacteria , *PROMOTERS (Genetics) , *TOBACCO - Abstract
The TIFY gene family plays an essential role in plant development and abiotic and biotic stress responses. In this study, genome-wide identification of TIFY members in tobacco and their expression pattern analysis in response to Ralstonia solanacearum infection were performed. A total of 33 TIFY genes were identified, including the TIFY, PPD, ZIM&ZML and JAZ subfamilies. Promoter analysis results indicated that a quantity of light-response, drought-response, SA-response and JA-response cis -elements exist in promoter regions. The TIFY gene family exhibited expansion and possessed gene redundancy resulting from tobacco ploidy change. In addition, most NtTIFY s equivalently expressed in roots, stems and leaves, while NtTIFY1 , NtTIFY4 , NtTIFY18 and NtTIFY30 preferentially expressed in roots. The JAZ III clade showed significant expression changes after inoculation with R. solanacearum , and the expression of NtTIFY7 in resistant varieties, compared with susceptible varieties, was more stably induced. Furthermore, NtTIFY7 -silenced plants, compared with the control plants, were more susceptible to bacterial wilt. These results lay a foundation for exploring the evolutionary history of TIFY gene family and revealing gene function of NtTIFY s in tobacco bacterial wilt resistance. • Thirty-three NtTIFY genes were identified in tobacco and grouped into four subfamilies. • The JAZ III clade was highly induced under the infection of R. solanacearum and treatment with Me-JA. • NtTIFY7 contributes to resistance against bacterial wilt in tobacco. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
12. Transcriptomics and virus-induced gene silencing identify defence-related genes during Ralstonia solanacearum infection in resistant and susceptible tobacco.
- Author
-
Xiao, Zhiliang, Liu, Zhengwen, Zhang, Huifen, Yang, Aiguo, Cheng, Lirui, Liu, Dan, Jiang, Caihong, Yu, Shizhou, Yang, Zhixiao, Ren, Min, and Geng, Ruimei
- Subjects
- *
BACTERIAL wilt diseases , *RALSTONIA solanacearum , *GENE silencing , *TRANSCRIPTOMES , *TOBACCO , *DRUG resistance in bacteria - Abstract
Bacterial wilt (BW) caused by Ralstonia solanacearum is a globally prevalent bacterial soil-borne disease. In this study, transcriptome sequencing were subjected to roots after infection with the R. solanacearum in the resistant and susceptible tobacco variety. DEGs that responded to R. solanacearum infection in both resistant and susceptible tobacco contributed to pectinase and peroxidase development and were enriched in plant hormone signal transduction, signal transduction and MAPK signalling pathway KEGG terms. Core DEGs in the resistant tobacco response to R. solanacearum infection were enriched in cell wall, membrane, abscisic acid and ethylene terms. qRT-PCR indicated that Nitab4.5_0004899g0110 , Nitab4.5_0004234g0080 and Nitab4.5_0001439g0050 contributed to the response to R. solanacearum infection in different resistant and susceptible tobacco. Silencing the p450 gene Nitab4.5_0001439g0050 reduced tobacco resistance to bacterial wilt. These results improve our understanding of the molecular mechanism of BW resistance in tobacco and solanaceous plants. • DEGs that responded to R. solanacearum infection in both resistant and susceptible tobacco contributed to pectinase and peroxidase development and were enriched in plant hormone signal transduction, signal transduction and MAPK signalling pathway KEGG terms. • Core DEGs in the resistant tobacco response to R. solanacearum infection were enriched in cell wall, membrane, abscisic acid and ethylene terms. • Silencing the p450 gene Nitab4.5_0001439g0050 reduced tobacco resistance to bacterial wilt. These results improve our understanding of the molecular mechanism of BW resistance in tobacco and solanaceous plants. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.