Search

The general aim of the project SUS&LOW is to increase the sustainability of fresh produce by testing and implementing lowinput agricultural practices (LIP) with positive impact on product quality with the support of nondestructive (ND) tools for realtime quality assessment and for product discrimination. Additionally, new marketing strategies are generated to better support the added value of the products and to satisfy the final consumers' preferences. The SUS&LOW project consists of three work packages (WP) and the adopted methodology used two model crops: rocket salad and tomato. The WP1, focused on the reduction of agricultural inputs, showed that sensorbased fertigation management might improve sustainability of soilless cultivation. Results coming from WP2, aimed to the evaluation of ND techniques, outlined the high potentiality of hyperspectral imaging (HSI) and Fourier transformednear infrared (FTNIR) techniques for the authentication of sustainable growing methods. Moreover, project activities' proved computer vision system (CVS) as an effective tool for evaluating the product quality also through the bag. The WP3, dealing with marketing strategies, indicated a positive approach of consumers compared to LIP products certified through a visual storytelling platform. [ABSTRACT FROM AUTHOR]

Table grapes are characterized by high susceptibility to mold development during postharvest, mostly due to Botrytis cinerea. Microwave application on readytoeat product can represent an alternative to antifungal treatment. With the aim of identifying the maximum energy that can be applied on grape without detrimental effects a Central Composite Design was developed testing the application of 10 combinations of treatment time (seconds) and microwave power (Watt). As a result, energies above 8000 kJ negatively affected the sensorial quality of fresh product, both in the presence and absence of B. cinerea inoculum (106 log CFU g1). The physicochemical parameters did not show significant differences, but two time/power combinations improved sensory quality of table grape, being selected for the subsequent packaging trial: 14 s/100 W and 80 s/100 W. Treatments were applied before or after packaging in polypropylene bags. At the end of storage period, 100 W applied for 80 seconds before packaging led to a better external appearance of the product than the other treatments, maintaining an intermediate level of mesophilic bacterial load and no significant differences in terms of nutritional quality. 80 seconds at 100 Watt combined with packaging can represent a valuable starting point for further experiments. [ABSTRACT FROM AUTHOR]

Copyright of Archeomatica is the property of MediaGEO and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study was aimed to investigate the quality at harvest and during storage of organically and conventionally grown 'Italia' grapes, collected from 2 different locations in Southern Italy. Four vineyards were chosen in order to have an organic and a conventional farm in each location. Before harvest, six plants per vineyard were randomly selected and considered as treatment replicate. Three bunches were harvested and labelled from each plant. In laboratory each bunch was weighed and thirty berries per bunch were detached and used for initial determination which included morphological (berry weight and dimension, peel thickness) and physical (berry color and firmness) attributes, maturity indices (respiration rate, soluble solids content and titratable acidity), and nutritional composition (phenol content, antioxidant activity, sugar and organic acid composition, ascorbic acid content). Then, the bunches from each replicate were kept in individual 15L jars at 0°C and connected to a humidified air flow throughout the whole experiment. After 7 and 14 days of storage, respiration rate, weight loss, physical and nutritional attributes were also monitored on 20 berries per bunch. Location and agricultural practices affected to a different extent several grapes quality attributes, both at harvest and during storage. Maturity stage, sugar content and berry color were significantly affected by the location, while antioxidantrelated compounds were significantly higher in organic grapes. Plant production and bunch weight were significantly higher for conventionally grown grapes, which also received the highest evaluation of external appearance, in terms of stalk dehydration and berry general aspect. Differences among conventional and organic grapes were maintained, for each location, during storage at 0°C. Conventional grapes maintained a higher visual quality during storage, resulting after 14 days below the limit of marketability (score 3) but above the edibility limit (score 2); whereas in one location organic grapes were judged not edible. Results showed a higher nutritional value in grapes obtained with the organic farming system although in terms of visual quality, storability and yield, conventional fruit had a better performance. [ABSTRACT FROM AUTHOR]

Wounding makes fresh-cut product more perishable than whole fruit. The effect of wounding intensity on respiration rate and nutritional quality of fresh-cut 'Candonga' strawberries was investigated. Fruit were submitted to six levels of cutting intensity - whole fruit (WHO), 4, 16, 64, and 128 pieces and chopped (CHO) samples. Respiration rate, and the main nutritional parameters were evaluated at the processing day and after 2 days of storage at 5°C. Results showed that wounding intensity significantly influenced respiration rate, ascorbic and dehydroascorbic acids, total phenolic content, and antioxidant capacity. Respiration rate increased with wounding intensity up to the level of 64 pieces (10.01 μg kg-1 s-1) compared to WHO (5.5 μg kg-1 s-1) and then decreased in the CHO samples (2.81 μg kg-1 s-1). At Day 2, the stress caused by the high intensity of cutting (64 pieces and CHO) induced a higher degradation of ascorbic acid, phenolic compounds, and antioxidant capacity. Stress-related changes decrease when the wounding damage was so high that it completely compromises the functionality of the cells (from 64 pieces up). These results should be considered for processing and packaging optimization of minimally processed strawberries-based products. [ABSTRACT FROM AUTHOR]

'Catanese' artichoke quarters were packaged in active modified atmosphere (5% O2+10% CO2) in four different materials or in air in macro-perforated bags used as control (CTRL), and stored at 4°C. Materials used for modified atmosphere packaging (MAP) were: polylactic acid (PLA), polylactid acid with one line of micro-perforation (PLA MF1), polypropilene with two lines of micro-perforations (PP MF2), and polypropylene + polyamide with two lines of micro-perforations (PP+PA MF2). Initially and after 2, 4, and 9 days, overall artichoke appearance, color and weight loss were monitored. O2 and CO2 concentrations within the packages were detected initially and after 1, 2, 17, 24.5, 41.5, 49.5, 120 and 216 h (end of the experiment). Also, at the last sampling date ethanol and acetaldehyde accumulations in artichoke tissue were measured. All the micro-perforated films maintained gas levels within the range of O2>3% and CO2<15%, defined as "safe", with a positive effect on quality: all samples remained above the limit of marketability until the end of the experiment, without significant differences among them, but showed a slight better overall appearance and, accordingly, a better retention of color parameters when compared with CTRL samples. Complete anaerobic condition (16% CO2 and 0% O2) developed in PLA bags where blackening of cut bracts and receptacle was observed, while black spots appeared on outer bracts, causing drastic quality reduction; samples fell below the limit of marketability after just 2 days. Also a significant accumulation of ethanol and acetaldehyde was found in these samples. Optimizing MAP made it possible to maintain the desired gas condition, with positive effects on quality of the produce in absence of any stabilizing treatment, for 9 days. [ABSTRACT FROM AUTHOR]

Exciting advances in technologies to measure biological systems are currently at the forefront of research. The ability to gather data along an increasing number of omic dimensions has created a need for tools to analyze all of this information together, rather than siloing each technology into separate analysis pipelines. To advance this goal, we introduce a framework called the single-cell multi-modal generative adversarial network (scMMGAN) that integrates data from multiple modalities into a unified representation in the ambient data space for downstream analysis using a combination of adversarial learning and data geometry techniques. The framework's key improvement is an additional diffusion geometry loss with a new kernel that constrains the otherwise over-parameterized GAN. We demonstrate scMMGAN's ability to produce more meaningful alignments than alternative methods on a wide variety of data modalities and that its output can be used to draw conclusions from real-world biological experimental data., Competing Interests: The authors declare no competing interests., (© 2022 The Author(s).)

Often when biological entities are measured in multiple ways, there are distinct categories of information: some information is easy-to-obtain information (EI) and can be gathered on virtually every subject of interest, while other information is hard-to-obtain information (HI) and can only be gathered on some. We propose building a model to make probabilistic predictions of HI using EI. Our feature mapping GAN (FMGAN), based on the conditional GAN framework, uses an embedding network to process conditions as part of the conditional GAN training to create manifold structure when it is not readily present in the conditions. We experiment on generating RNA sequencing of cell lines perturbed with a drug conditioned on the drug's chemical structure and generating FACS data from clinical monitoring variables on a cohort of COVID-19 patients, effectively describing their immune response in great detail., Competing Interests: The authors declare no competing interests., (© 2021 The Authors.)

The genus Flavivirus contains many mosquito-borne human pathogens of global epidemiological importance such as dengue virus, West Nile virus, and Zika virus, which has recently emerged at epidemic levels. Infections with these viruses result in divergent clinical outcomes ranging from asymptomatic to fatal. Myriad factors influence infection severity including exposure, immune status and pathogen/host genetics. Furthermore, pre-existing infection may skew immune pathways or divert immune resources. We profiled immune cells from dengue virus-infected individuals by multiparameter mass cytometry (CyTOF) to define functional status. Elevations in IFNβ were noted in acute patients across the majority of cell types and were statistically elevated in 31 of 36 cell subsets. We quantified response to in vitro (re)infection with dengue or Zika viruses and detected a striking pattern of upregulation of responses to Zika infection by innate cell types which was not noted in response to dengue virus. Significance was discovered by statistical analysis as well as a neural network-based clustering approach which identified unusual cell subsets overlooked by conventional manual gating. Of public health importance, patient cells showed significant enrichment of innate cell responses to Zika virus indicating an intact and robust anti-Zika response despite the concurrent dengue infection., Competing Interests: The authors have declared that no competing interests exist.

An annual monitoring campaign of VOCs, consisting of twelve sampling periods, was carried out from June 2008 to June 2009 in Modugno, a city located in the Apulia region (Southern Italy), in order to assess the urban air quality, identify the main emission sources, and quantify the cancer and no-cancer risk attributable to inhalation exposures. Monitoring, carried out by using the Radiello diffusive samplers, was conducted in eleven sampling sites throughout the city taking into account the traffic density and the architecture of the city. From the study of the data, it was found that, among all considered VOCs, benzene, toluene, ethylbenzene, and xylenes (BTEX) are the pollutants at higher concentration. The analysis of VOC concentrations, the study of the topography of the city, and the use of different diagnostic ratios between the BTEX species showed that the vehicular traffic emissions were the predominant source of VOCs in the urban area of Modugno. Despite that the annual concentration of benzene is lower than the regulatory limit, the estimation of cancer risk showed that the global lifetime cancer risk attributed to the investigated VOC exposure was not negligible and therefore should be taken into account in future regulatory approaches.

The present work studied how much the meteorological parameters and the emission sources can influence the particulate polycyclic aromatic hydrocarbons (Invalid Journal Information PAHs) concentrations in two areas located in Southern Italy (Bari and Taranto). It was found that when the vehicular traffic is the main source of PAHs, there is a negative correlation between ambient temperature, wind speed and PAHs concentration (Bari). This is because these parameters are generally correlated with the dispersion capacity of the atmosphere. In the presence of a large industrial area, the wind direction becomes an important parameter able to determinate large changes in PAHs concentrations. This happened in Taranto where PAHs concentrations are exceptionally high. During the study the seasonal trend of particulate PAHs and PM10 was compared. PM10 did not show a significant seasonal cycle during the year because it is conditioned from a high regional aerosol background, especially during the summertime. On the contrary, particulate PAHs exerted distinct seasonal variation with higher concentrations in the winter and lower concentration during other months of the year. This evidence suggested that PAHs concentrations can be considered a more reliable index for air-quality assessment. In order to identify an index that considers the contributions of other particulate PAHs, it is necessary to calculate the carcinogenic potency of total PAHs (i.e., total BaPeq) obtained by the sum of the benzo[a]pyrene equivalent concentration (BaPeq) for each PAH.