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2. Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity.

Author

Alvarez, Gabriel, Villanueva, Sofia, Breininger, Elizabeth, Geller, Marisa, Ruhlmann, Claudio, Dalvit, Gabriel, Cetica, Pablo, and Masashige Kuwayama

Subjects
SPERMATOZOA, OVUM, INTRACYTOPLASMIC sperm injection, BLASTOCYST, HUMAN embryology, BOS, PHOSPHOLIPASE C, ACTIVATION (Chemistry)
Abstract

Background: The intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors. Aim: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with the phospholipase C zeta (PLCɀ) activity of both species. Methods: In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples. Results: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages and ICSI + chemical activation presented the highest 2 PN, cleavage, and blastocyst rates (p < 0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (p < 0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (p < 0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm (p < 0.05). Conclusion: Our results suggest that the higher stability of the bovine sperm in combination with its relatively low content of PLCɀ impairs bovine oocyte activation after ICSI. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Comparing two simple cryopreservation methods for porcine sperm: Effect on sperm quality parameters

Author

Perez, M.C, Petrinelli, A., Rodriguez, Pablo C., Satorre, María Mercedes, and Breininger, Elizabeth

Subjects
criopreservación, calidad seminal, purl.org/becyt/ford/4.4 [https], semen, porcino, purl.org/becyt/ford/4 [https]
Abstract

Cerca del 40% de la carne roja que se consume en el mundo es provista por los cerdos. Los productores porcinos de nuestro país se encuentran ante el enorme desafío de lograr mejoras productivas con un desarrollo económico sustentable y eficiente, así como implementar tecnologías reproductivas, como la inseminación artificial y la criopreservación de semen. Tales adelantos aún presentan enormes problemas en estaespecie. El objetivo de este trabajo fue comparar dos métodos simples de congelamiento para semen porcino, evaluando los parámetros de calidad seminal al momento del descongelado. Las muestras de semen fueron criopreservadas en pajuelas siguiendo el protocolo descripto por Peña y colaboradores en 2003, realizando (grupo tratamiento) o no (grupo control) una modificación en el paso final de congelamiento posterior al envasado en las pajuelas. Las evaluaciones de motilidad y vitalidad se realizaron por microscopía óptica en platina termostatizada y por las técnicas de eosina/nigrosina y la combinación del colorante supravital azul tripán con microscopía de contraste interferencial diferencial, respectivamente. La criocapacitación se evaluó por la técnica fluorescente de clorotetraciclina (CTC). Los resultados de los diferentes experimentos fueron analizados por la prueba t de Student. Las muestras del grupo tratamiento evidenciaron una motilidad significativamente más alta (p

Published
2019

4. Study of sperm evaluation parameters to estimate cryopreserved bovine semen fertility

Author

Morado, Sergio Adrián, Pereyra, V., Breininger, Elizabeth, Sara, R., and Cetica, Pablo Daniel

Subjects
Semen fertility, CIENCIAS AGRÍCOLAS, Otras Ciencias Veterinarias, Ciencias Veterinarias, In vitro fertilization, Bull sperm, purl.org/becyt/ford/4.3 [https], Functional parameters, purl.org/becyt/ford/4 [https]
Abstract

In cattle production, reproductive biotechnologies such as artificial insemination with Cryopreserved semen samples show the same performance as insemination with fresh semen. The most representative method to evaluate semen fertilizing capability is through the results obtained by in vivo fertility. However, as it is difficult to apply this method in practice, routine laboratory tests can be used to evaluate semen quality. The aim of this work was to evaluate functional spermatic parameters in cryopreserved bovine semen samples from males presenting high and low field fertility rates. Semen samples were obtained from bulls presenting low or high fertility in a program of artificial insemination. Routine and functional parameters were determined in both groups. As regards routine tests, no significant differences were observed in progressive motility and vigor between samples of animals with high and low fertility in vivo, but differences were detected in vitality and acrosome integrity (p

Published
2015

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