43 results on '"Byrne, Deborah"'
Search Results
2. Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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- 2022
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3. Uridine diphosphate N-acetylglucosamine orchestrates the interaction of GlmR with either YvcJ or GlmS in Bacillus subtilis
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Foulquier, Elodie, Pompeo, Frédérique, Byrne, Deborah, Fierobe, Henri-Pierre, and Galinier, Anne
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- 2020
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4. YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coli
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Germain, Elsa, Guiraud, Paul, Byrne, Deborah, Douzi, Badreddine, Djendli, Meriem, and Maisonneuve, Etienne
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- 2019
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5. Cold adaptation in the environmental bacterium Shewanella oneidensis is controlled by a J-domain co-chaperone protein network
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Maillot, Nathanael Jean, Honoré, Flora Ambre, Byrne, Deborah, Méjean, Vincent, and Genest, Olivier
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- 2019
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6. A qualitative analysis of women's postnatal experiences of breastfeeding supports during the perinatal period in Ireland.
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Lawlor, Niamh, Prihodova, Lucia, Byrne, Deborah, Etherton, Megan, Rahill, Felicienne, Wilson, Catie, and O'Sullivan, Elizabeth J.
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BREASTFEEDING ,PERINATAL period ,CHILDBIRTH at home ,PUERPERIUM ,MEDICAL personnel ,THEMATIC analysis ,OPEN-ended questions - Abstract
Ireland has among the lowest rates of breastfeeding worldwide. Despite policies to support breastfeeding, breastfeeding initiation and exclusivity remain low in Ireland. Greater knowledge about support received in the maternity unit may—in part—shed light on why this is so. Our aim was to analyse women's experiences of the breastfeeding supports available in the early postnatal period in Ireland. We conducted an analysis of an open-ended question on a cross-sectional survey about breastfeeding support conducted in the Republic of Ireland in 2022. Participants were asked to provide comments about the breastfeeding support they received in the maternity unit or during your home birth. Data were analysed using Braun and Clarke's six-step Thematic Analysis Framework. There were 5,412 unique responses to the survey and 2,264 responses to the question of interest. Two themes were generated from the data: (i) 'Breastfeeding support in theory but not in practice.' Although breastfeeding was promoted by healthcare professionals antenatally, breastfeeding challenges were rarely mentioned. Participants then felt unsupported in overcoming challenges postnatally. (ii) 'Support was either inaccessible due to lack of staff/time, inadequate; i.e., unhelpful or non-specific, and/or physically inappropriate.' Most participants described receiving supports that were less than optimal in aiding them to establish breastfeeding. While many described difficulties in accessing supports, others found support to be 'non-specific,' 'rushed' and sometimes 'rough.' A lack of knowledge, time and support from healthcare professionals was frequently described, which was often recognised as a failing of the healthcare system. Women require practical, informative, and specific breastfeeding support. Barriers such as lack of time and trained staff in the maternity unit need to be addressed. [ABSTRACT FROM AUTHOR]
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- 2023
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7. Salmonella antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of EF-Tu P-loop
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Jurėnas, Dukas, primary, Rey, Martial, additional, Byrne, Deborah, additional, Chamot-Rooke, Julia, additional, Terradot, Laurent, additional, and Cascales, Eric, additional
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- 2022
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8. Study of human Cytochrome P450 reductase by Electron Paramagnetic Resonance
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Bizet, Maxime, Byrne, Deborah, Gerbaud, Guillaume, Biaso, Frédéric, Dorlet, Pierre, Urban, Philippe, Truan, Gilles, Martinho, Marlène, Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Protein Expression and Purification Core Facility, European Molecular Biology Laboratory, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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Biophysics ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Cell Biology ,Biochemistry - Abstract
International audience; Human cytochrome P450 reductase is a membrane protein located on the cytoplasmic side of the endoplasmic reticulum. It contains two binding domains for FAD and FMN, which are connected by a flexible region [1]. CPR transfers electrons from NADPH to cytochromes P450 through flavins. Two crystallographic structures were obtained for Rattus norvegicus showing "closed" and "open" conformations [2] These two structures show different distances between the flavin cofactors. Indeed, for inter-flavin Electronic Transfer (ET), the two FAD and FMN domains must be nearby (a "closed" conformation), but inter-protein ET (FMN to CYPs) requires a more "open" conformation. The understanding and characterization of domain movement associated with ET is essential and will be studied by measuring distances between two paramagnetic centers via two approaches: 1) using endogenous probes by isolating the flavin radical, and 2) by Site-Directed Spin Labeling and EPR, based on the insertion of paramagnetic nitroxide probes. Classically, these are specific to cysteines [3]. However, C566 is involved in the enzymatic activity and cannot be modified. The incorporation of non-natural amino acids and their labelling with specific nitroxides has been shown to be a powerful alternative [4] First experiments on human soluble CPR concerning the labelling of non-natural amino acids, as well as the trapping of flavin centers will be presented.
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- 2022
9. Uncoupling the Hsp90 and DnaK chaperone activities revealed the in vivo relevance of their collaboration in bacteria
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Corteggiani, Marie, primary, Bossuet-Greif, Nadège, additional, Nougayrède, Jean-Philippe, additional, Byrne, Deborah, additional, Ilbert, Marianne, additional, Dementin, Sébastien, additional, Giudici-Orticoni, Marie-Thérèse, additional, Méjean, Vincent, additional, Oswald, Eric, additional, and Genest, Olivier, additional
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- 2022
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10. Additional file 5 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 5. Kinetic analysis of XuaD, XuaE, XuaH and XuaJ with various ligands are presented.
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- 2022
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11. Additional file 3 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 3. Analysis of the digestion products released by XuaD and XuaE from AXOS. Chromatograms obtained after High Pressure Anion Exchange Chromatography coupled with Pulsed Amperometric Detection (HPAEC–PAD) are presented.
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- 2022
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12. Additional file 8 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 8. Strains and vectors used in this study. Table with strains and vectors used in this study and their relevant characteristics and sources and references are presented.
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- 2022
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13. Additional file 7 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 7. Growth of R. cellulolyticum wild-type, mutant and derivatives strains on arabinose. Growth curves are presented.
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- 2022
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14. Additional file 6 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 6. Molecular analysis of the Ruminiclostridium cellulolyticum mutant strain. PCR and southern blot analysis on the wild-type and the mutant strains are presented.
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- 2022
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15. Additional file 9 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 9. Primers used in this study. Table with the primers used in the study and their sequence are presented.
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- 2022
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16. Additional file 1 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 1. Interaction of XuaA with various carbohydrate ligands using thermal shift assay is presented.
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- 2022
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17. Additional file 2 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 2. Interaction of XuaA with various carbohydrates using isothermal titration calorimetry is presented.
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- 2022
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18. Additional file 4 of Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow
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Liu, Nian, Gagnot, Séverine, Denis, Yann, Byrne, Deborah, Faulds, Craig, Fierobe, Henri-Pierre, and Perret, Stéphanie
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Additional file 4. Analysis of the digestion products released by XuaF and XuaG from AXOS and XOS. Chromatograms obtained after High Pressure Anion Exchange Chromatography coupled with Pulsed Amperometric Detection (HPAEC–PAD) are presented.
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- 2022
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19. Acidithiobacillus ferrooxidans oxidizes ferrous iron before sulfur likely through transcriptional regulation by the global redox responding RegBA signal transducing system
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Ponce, José Sandoval, Moinier, Danielle, Byrne, Deborah, Amouric, Agnès, and Bonnefoy, Violaine
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- 2012
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20. Structure and Evolution of the Ivy Protein Family, Unexpected Lysozyme Inhibitors in Gram-Negative Bacteria
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Abergel, Chantal, Monchois, Vincent, Byrne, Deborah, Chenivesse, Sabine, Lembo, Frédérique, Lazzaroni, Jean-Claude, and Claverie, Jean-Michel
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- 2007
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21. Mechanism of Zn2+ and Ca2+ Binding to Human S100A1
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Baksheeva, Viktoriia E., primary, Roman, Andrei Yu., additional, Villard, Claude, additional, Devred, François, additional, Byrne, Deborah, additional, Yatoui, Dahbia, additional, Zalevsky, Arthur O., additional, Vologzhannikova, Alisa A., additional, Sokolov, Andrey S., additional, Permyakov, Sergei E., additional, Golovin, Andrey V., additional, Shaw, Gary S., additional, Tsvetkov, Philipp O., additional, and Zernii, Evgeni Yu., additional
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- 2021
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22. NirD curtails the stringent response by inhibiting RelA activity in Escherichia coli
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Léger, Loïc, primary, Byrne, Deborah, additional, Guiraud, Paul, additional, Germain, Elsa, additional, and Maisonneuve, Etienne, additional
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- 2021
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23. Effect of Hydrocortisone on Mortality and Organ Support in Patients With Severe COVID-19:The REMAP-CAP COVID-19 Corticosteroid Domain Randomized Clinical Trial
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Angus, Derek C, Derde, Lennie, Al-Beidh, Farah, Annane, Djillali, Arabi, Yaseen, Beane, Abigail, van Bentum-Puijk, Wilma, Berry, Lindsay, Bhimani, Zahra, Bonten, Marc, Bradbury, Charlotte, Brunkhorst, Frank, Buxton, Meredith, Buzgau, Adrian, Cheng, Allen C, de Jong, Menno, Detry, Michelle, Estcourt, Lise, Fitzgerald, Mark, Goossens, Herman, Green, Cameron, Haniffa, Rashan, Higgins, Alisa M, Horvat, Christopher, Hullegie, Sebastiaan J, Kruger, Peter, Lamontagne, Francois, Lawler, Patrick R, Linstrum, Kelsey, Litton, Edward, Lorenzi, Elizabeth, Marshall, John, McAuley, Daniel, McGlothin, Anna, McGuinness, Shay, McVerry, Bryan, Montgomery, Stephanie, Mouncey, Paul, Murthy, Srinivas, Nichol, Alistair, Parke, Rachael, Parker, Jane, Rowan, Kathryn, Sanil, Ashish, Santos, Marlene, Saunders, Christina, Seymour, Christopher, Turner, Anne, van de Veerdonk, Frank, Venkatesh, Balasubramanian, Zarychanski, Ryan, Berry, Scott, Lewis, Roger J, McArthur, Colin, Webb, Steven A, Gordon, Anthony C, Writing Committee for the REMAP-CAP Investigators, Angus, Derek, Cheng, Allen, De Jong, Menno, Gordon, Anthony, Lawler, Patrick, Webb, Steve, Campbell, Lewis, Forbes, Andrew, Gattas, David, Heritier, Stephane, Higgins, Lisa, Peake, Sandra, Presneill, Jeffrey, Seppelt, Ian, Trapani, Tony, Young, Paul, Bagshaw, Sean, Daneman, Nick, Ferguson, Niall, Misak, Cheryl, Hullegie, Sebastiaan, Pletz, Mathias, Rohde, Gernot, Rowan, Kathy, Alexander, Brian, Basile, Kim, Girard, Timothy, Huang, David, Vates, Jennifer, Beasley, Richard, Fowler, Robert, McGloughlin, Steve, Morpeth, Susan, Paterson, David, Venkatesh, Bala, Uyeki, Tim, Baillie, Kenneth, Duffy, Eamon, Fowler, Rob, Hills, Thomas, Orr, Katrina, Patanwala, Asad, Tong, Steve, Netea, Mihai, Bihari, Shilesh, Carrier, Marc, Fergusson, Dean, Goligher, Ewan, Haidar, Ghady, Hunt, Beverley, Kumar, Anand, Laffan, Mike, Lawless, Patrick, Lother, Sylvain, McCallum, Peter, Middeldopr, Saskia, McQuilten, Zoe, Neal, Matthew, Pasi, John, Schutgens, Roger, Stanworth, Simon, Turgeon, Alexis, Weissman, Alexandra, Adhikari, Neill, Anstey, Matthew, Brant, Emily, de Man, Angelique, Lamonagne, Francois, Masse, Marie-Helene, Udy, Andrew, Arnold, Donald, Begin, Phillipe, Charlewood, Richard, Chasse, Michael, Coyne, Mark, Cooper, Jamie, Daly, James, Gosbell, Iain, Harvala-Simmonds, Heli, Hills, Tom, MacLennan, Sheila, Menon, David, McDyer, John, Pridee, Nicole, Roberts, David, Shankar-Hari, Manu, Thomas, Helen, Tinmouth, Alan, Triulzi, Darrell, Walsh, Tim, Wood, Erica, Calfee, Carolyn, O’Kane, Cecilia, Shyamsundar, Murali, Sinha, Pratik, Thompson, Taylor, Young, Ian, Bihari, Shailesh, Hodgson, Carol, Laffey, John, McAuley, Danny, Orford, Neil, Neto, Ary, Lewis, Roger, McGlothlin, Anna, Miller, Eliza, Singh, Vanessa, Zammit, Claire, van Bentum Puijk, Wilma, Bouwman, Wietske, Mangindaan, Yara, Parker, Lorraine, Peters, Svenja, Rietveld, Ilse, Raymakers, Kik, Ganpat, Radhika, Brillinger, Nicole, Markgraf, Rene, Ainscough, Kate, Brickell, Kathy, Anjum, Aisha, Lane, Janis-Best, Richards-Belle, Alvin, Saull, Michelle, Wiley, Daisy, Bion, Julian, Connor, Jason, Gates, Simon, Manax, Victoria, van der Poll, Tom, Reynolds, John, van Beurden, Marloes, Effelaar, Evelien, Schotsman, Joost, Boyd, Craig, Harland, Cain, Shearer, Audrey, Wren, Jess, Clermont, Giles, Garrard, William, Kalchthaler, Kyle, King, Andrew, Ricketts, Daniel, Malakoutis, Salim, Marroquin, Oscar, Music, Edvin, Quinn, Kevin, Cate, Heidi, Pearson, Karen, Collins, Joanne, Hanson, Jane, Williams, Penny, Jackson, Shane, Asghar, Adeeba, Dyas, Sarah, Sutu, Mihaela, Murphy, Sheenagh, Williamson, Dawn, Mguni, Nhlanhla, Potter, Alison, Porter, David, Goodwin, Jayne, Rook, Clare, Harrison, Susie, Williams, Hannah, Campbell, Hilary, Lomme, Kaatje, Williamson, James, Sheffield, Jonathan, van’t Hoff, Willian, McCracken, Phobe, Young, Meredith, Board, Jasmin, Mart, Emma, Knott, Cameron, Smith, Julie, Boschert, Catherine, Affleck, Julia, Ramanan, Mahesh, D’Souza, Ramsy, Pateman, Kelsey, Shakih, Arif, Cheung, Winston, Kol, Mark, Wong, Helen, Shah, Asim, Wagh, Atul, Simpson, Joanne, Duke, Graeme, Chan, Peter, Cartner, Brittney, Hunter, Stephanie, Laver, Russell, Shrestha, Tapaswi, Regli, Adrian, Pellicano, Annamaria, McCullough, James, Tallott, Mandy, Kumar, Nikhil, Panwar, Rakshit, Brinkerhoff, Gail, Koppen, Cassandra, Cazzola, Federica, Brain, Matthew, Mineall, Sarah, Fischer, Roy, Biradar, Vishwanath, Soar, Natalie, White, Hayden, Estensen, Kristen, Morrison, Lynette, Smith, Joanne, Cooper, Melanie, Health, Monash, Shehabi, Yahya, Al-Bassam, Wisam, Hulley, Amanda, Whitehead, Christina, Lowrey, Julie, Gresha, Rebecca, Walsham, James, Meyer, Jason, Harward, Meg, Venz, Ellen, Williams, Patricia, Kurenda, Catherine, Smith, Kirsy, Smith, Margaret, Garcia, Rebecca, Barge, Deborah, Byrne, Deborah, Byrne, Kathleen, Driscoll, Alana, Fortune, Louise, Janin, Pierre, Yarad, Elizabeth, Hammond, Naomi, Bass, Frances, Ashelford, Angela, Waterson, Sharon, Wedd, Steve, McNamara, Robert, Buhr, Heidi, Coles, Jennifer, Schweikert, Sacha, Wibrow, Bradley, Rauniyar, Rashmi, Myers, Erina, Fysh, Ed, Dawda, Ashlish, Mevavala, Bhaumik, Litton, Ed, Ferrier, Janet, Nair, Priya, Buscher, Hergen, Reynolds, Claire, Santamaria, John, Barbazza, Leanne, Homes, Jennifer, Smith, Roger, Murray, Lauren, Brailsford, Jane, Forbes, Loretta, Maguire, Teena, Mariappa, Vasanth, Smith, Judith, Simpson, Scott, Maiden, Matthew, Bone, Allsion, Horton, Michelle, Salerno, Tania, Sterba, Martin, Geng, Wenli, Depuydt, Pieter, De Waele, Jan, De Bus, Liesbet, Fierens, Jan, Bracke, Stephanie, Reeve, Brenda, Dechert, William, Chassé, Michaël, Carrier, François Martin, Boumahni, Dounia, Benettaib, Fatna, Ghamraoui, Ali, Bellemare, David, Cloutier, Ève, Francoeur, Charles, Lamontagne, François, D’Aragon, Frédérick, Carbonneau, Elaine, Leblond, Julie, Vazquez-Grande, Gloria, Marten, Nicole, Wilson, Maggie, Albert, Martin, Serri, Karim, Cavayas, Alexandros, Duplaix, Mathilde, Williams, Virginie, Rochwerg, Bram, Karachi, Tim, Oczkowski, Simon, Centofanti, John, Millen, Tina, Duan, Erick, Tsang, Jennifer, Patterson, Lisa, English, Shane, Watpool, Irene, Porteous, Rebecca, Miezitis, Sydney, McIntyre, Lauralyn, Brochard, Laurent, Burns, Karen, Sandhu, Gyan, Khalid, Imrana, Binnie, Alexandra, Powell, Elizabeth, McMillan, Alexandra, Luk, Tracy, Aref, Noah, Andric, Zdravko, Cviljevic, Sabina, Đimoti, Renata, Zapalac, Marija, Mirković, Gordan, Baršić, Bruno, Kutleša, Marko, Kotarski, Viktor, Vujaklija Brajković, Ana, Babel, Jakša, Sever, Helena, Dragija, Lidija, Kušan, Ira, Vaara, Suvi, Pettilä, Leena, Heinonen, Jonna, Kuitunen, Anne, Karlsson, Sari, Vahtera, Annukka, Kiiski, Heikki, Ristimäki, Sanna, Azaiz, Amine, Charron, Cyril, Godement, Mathieu, Geri, Guillaume, Vieillard-Baron, Antoine, Pourcine, Franck, Monchi, Mehran, Luis, David, Mercier, Romain, Sagnier, Anne, Verrier, Nathalie, Caplin, Cecile, Siami, Shidasp, Aparicio, Christelle, Vautier, Sarah, Jeblaoui, Asma, Fartoukh, Muriel, Courtin, Laura, Labbe, Vincent, Leparco, Cécile, Muller, Grégoire, Nay, Mai-Anh, Kamel, Toufik, Benzekri, Dalila, Jacquier, Sophie, Mercier, Emmanuelle, Chartier, Delphine, Salmon, Charlotte, Dequin, PierreFrançois, Schneider, Francis, Morel, Guillaume, L’Hotellier, Sylvie, Badie, Julio, Berdaguer, Fernando Daniel, Malfroy, Sylvain, Mezher, Chaouki, Bourgoin, Charlotte, Megarbane, Bruno, Voicu, Sebastian, Deye, Nicolas, Malissin, Isabelle, Sutterlin, Laetitia, Guitton, Christophe, Darreau, Cédric, Landais, Mickaël, Chudeau, Nicolas, Robert, Alain, Moine, Pierre, Heming, Nicholas, Maxime, Virginie, Bossard, Isabelle, Nicholier, Tiphaine Barbarin, Colin, Gwenhael, Zinzoni, Vanessa, Maquigneau, Natacham, Finn, André, Kreß, Gabriele, Hoff, Uwe, Friedrich Hinrichs, Carl, Nee, Jens, Hagel, Stefan, Ankert, Juliane, Kolanos, Steffi, Bloos, Frank, Petros, Sirak, Pasieka, Bastian, Kunz, Kevin, Appelt, Peter, Schütze, Bianka, Kluge, Stefan, Nierhaus, Axel, Jarczak, Dominik, Roedl, Kevin, Weismann, Dirk, Frey, Anna, Klinikum Neukölln, Vivantes, Reill, Lorenz, Distler, Michael, Maselli, Astrid, Bélteczki, János, Magyar, István, Fazekas, Ágnes, Kovács, Sándor, Szőke, Viktória, Szigligeti, Gábor, Leszkoven, János, Collins, Daniel, Breen, Patrick, Frohlich, Stephen, Whelan, Ruth, McNicholas, Bairbre, Scully, Michael, Casey, Siobhan, Kernan, Maeve, Doran, Peter, O’Dywer, Michael, Smyth, Michelle, Hayes, Leanne, Hoiting, Oscar, Peters, Marco, Rengers, Els, Evers, Mirjam, Prinssen, Anton, Bosch Ziekenhuis, Jeroen, Simons, Koen, Rozendaal, Wim, Polderman, F, de Jager, P, Moviat, M, Paling, A, Salet, A, Rademaker, Emma, Peters, Anna Linda, de Jonge, E, Wigbers, J, Guilder, E, Butler, M, Cowdrey, Keri-Anne, Newby, Lynette, Chen, Yan, Simmonds, Catherine, McConnochie, Rachael, Ritzema Carter, Jay, Henderson, Seton, Van Der Heyden, Kym, Mehrtens, Jan, Williams, Tony, Kazemi, Alex, Song, Rima, Lai, Vivian, Girijadevi, Dinu, Everitt, Robert, Russell, Robert, Hacking, Danielle, Buehner, Ulrike, Williams, Erin, Browne, Troy, Grimwade, Kate, Goodson, Jennifer, Keet, Owen, Callender, Owen, Martynoga, Robert, Trask, Kara, Butler, Amelia, Schischka, Livia, Young, Chelsea, Lesona, Eden, Olatunji, Shaanti, Robertson, Yvonne, José, Nuno, Amaro dos Santos Catorze, Teodoro, de Lima Pereira, Tiago Nuno Alfaro, Neves Pessoa, Lucilia Maria, Castro Ferreira, Ricardo Manuel, Pereira Sousa Bastos, Joana Margarida, Aysel Florescu, Simin, Stanciu, Delia, Zaharia, Miahela Florentina, Kosa, Alma Gabriela, Codreanu, Daniel, Marabi, Yaseen, Al Qasim, Eman, Moneer Hagazy, Mohamned, Al Swaidan, Lolowa, Arishi, Hatim, Muñoz-Bermúdez, Rosana, Marin-Corral, Judith, Salazar Degracia, Anna, Parrilla Gómez, Francisco, Mateo López, Maria Isabel, Rodriguez Fernandez, Jorge, Cárcel Fernández, Sheila, Carmona Flores, Rosario, León López, Rafael, de la Fuente Martos, Carmen, Allan, Angela, Polgarova, Petra, Farahi, Neda, McWilliam, Stephen, Hawcutt, Daniel, Rad, Laura, O’Malley, Laura, Whitbread, Jennifer, Kelsall, Olivia, Wild, Laura, Thrush, Jessica, Wood, Hannah, Austin, Karen, Donnelly, Adrian, Kelly, Martin, O’Kane, Sinéad, McClintock, Declan, Warnock, Majella, Johnston, Paul, Gallagher, Linda Jude, Mc Goldrick, Clare, Mc Master, Moyra, Strzelecka, Anna, Jha, Rajeev, Kalogirou, Michael, Ellis, Christine, Krishnamurthy, Vinodh, Deelchand, Vashish, Silversides, Jon, McGuigan, Peter, Ward, Kathryn, O’Neill, Aisling, Finn, Stephanie, Phillips, Barbara, Mullan, Dee, Oritz-Ruiz de Gordoa, Laura, Thomas, Matthew, Sweet, Katie, Grimmer, Lisa, Johnson, Rebekah, Pinnell, Jez, Robinson, Matt, Gledhill, Lisa, Wood, Tracy, Morgan, Matt, Cole, Jade, Hill, Helen, Davies, Michelle, Antcliffe, David, Templeton, Maie, Rojo, Roceld, Coghlan, Phoebe, Smee, Joanna, Mackay, Euan, Cort, Jon, Whileman, Amanda, Spencer, Thomas, Spittle, Nick, Kasipandian, Vidya, Patel, Amit, Allibone, Suzanne, Genetu, Roman Mary, Ramali, Mohamed, Ghosh, Alison, Bamford, Peter, London, Emily, Cawley, Kathryn, Faulkner, Maria, Jeffrey, Helen, Smith, Tim, Brewer, Chris, Gregory, Jane, Limb, James, Cowton, Amanda, O’Brien, Julie, Nikitas, Nikitas, Wells, Colin, Lankester, Liana, Pulletz, Mark, Birch, Jenny, Wiseman, Sophie, Horton, Sarah, Alegria, Ana, Turki, Salah, Elsefi, Tarek, Crisp, Nikki, Allen, Louise, McCullagh, Iain, Robinson, Philip, Hays, Carole, Babio-Galan, Maite, Stevenson, Hannah, Khare, Divya, Pinder, Meredith, Selvamoni, Selvin, Gopinath, Amitha, Pugh, Richard, Menzies, Daniel, Mackay, Callum, Allan, Elizabeth, Davies, Gwyneth, Puxty, Kathryn, McCue, Claire, Cathcart, Susanne, Hickey, Naomi, Ireland, Jane, Yusuff, Hakeem, Isgro, Graziella, Brightling, Chris, Bourne, Michelle, Craner, Michelle, Watters, Malcolm, Prout, Rachel, Davies, Louisa, Pegler, Suzannah, Kyeremeh, Lynsey, Arbane, Gill, Wilson, Karen, Gomm, Linda, Francia, Federica, Brett, Stephen, Sousa Arias, Sonia, Elin Hall, Rebecca, Budd, Joanna, Small, Charlotte, Birch, Janine, Collins, Emma, Henning, Jeremy, Bonner, Stephen, Hugill, Keith, Cirstea, Emanuel, Wilkinson, Dean, Karlikowski, Michal, Sutherland, Helen, Wilhelmsen, Elva, Woods, Jane, North, Julie, Sundaran, Dhinesh, Hollos, Laszlo, Coburn, Susan, Walsh, Joanne, Turns, Margaret, Hopkins, Phil, Smith, John, Noble, Harriet, Depante, Maria Theresa, Clarey, Emma, Laha, Shondipon, Verlander, Mark, Williams, Alexandra, Huckle, Abby, Hall, Andrew, Cooke, Jill, Gardiner-Hill, Caroline, Maloney, Carolyn, Qureshi, Hafiz, Flint, Neil, Nicholson, Sarah, Southin, Sara, Nicholson, Andrew, Borgatta, Barbara, Turner-Bone, Ian, Reddy, Amie, Wilding, Laura, Chamara Warnapura, Loku, Agno Sathianathan, Ronan, Golden, David, Hart, Ciaran, Jones, Jo, Bannard-Smith, Jonathan, Henry, Joanne, Birchall, Katie, Pomeroy, Fiona, Quayle, Rachael, Makowski, Arystarch, Misztal, Beata, Ahmed, Iram, KyereDiabour, Thyra, Naiker, Kevin, Stewart, Richard, Mwaura, Esther, Mew, Louise, Wren, Lynn, Willams, Felicity, Innes, Richard, Doble, Patricia, Hutter, Joanne, Shovelton, Charmaine, Plumb, Benjamin, Szakmany, Tamas, Hamlyn, Vincent, Hawkins, Nancy, Lewis, Sarah, Dell, Amanda, Gopal, Shameer, Ganguly, Saibal, Smallwood, Andrew, Harris, Nichola, Metherell, Stella, Lazaro, Juan Martin, Newman, Tabitha, Fletcher, Simon, Nortje, Jurgens, Fottrell-Gould, Deirdre, Randell, Georgina, Zaman, Mohsin, Elmahi, Einas, Jones, Andrea, Hall, Kathryn, Mills, Gary, Ryalls, Kim, Bowler, Helen, Sall, Jas, Bourne, Richard, Borrill, Zoe, Duncan, Tracey, Lamb, Thomas, Shaw, Joanne, Fox, Claire, Moreno Cuesta, Jeronimo, Xavier, Kugan, Purohit, Dharam, Elhassan, Munzir, Bakthavatsalam, Dhanalakshmi, Rowland, Matthew, Hutton, Paula, Bashyal, Archana, Davidson, Neil, Hird, Clare, Chhablani, Manish, Phalod, Gunjan, Kirkby, Amy, Archer, Simon, Netherton, Kimberley, Reschreiter, Henrik, Camsooksai, Julie, Patch, Sarah, Jenkins, Sarah, Pogson, David, Rose, Steve, Daly, Zoe, Brimfield, Lutece, Claridge, Helen, Parekh, Dhruv, Bergin, Colin, Bates, Michelle, Dasgin, Joanne, McGhee, Christopher, Sim, Malcolm, Hay, Sophie Kennedy, Henderson, Steven, Phull, Mandeep-Kaur, Zaidi, Abbas, Pogreban, Tatiana, Rosaroso, Lace Paulyn, Harvey, Daniel, Lowe, Benjamin, Meredith, Megan, Ryan, Lucy, Hormis, Anil, Walker, Rachel, Collier, Dawn, Kimpton, Sarah, Oakley, Susan, Rooney, Kevin, Rodden, Natalie, Hughes, Emma, Thomson, Nicola, McGlynn, Deborah, Walden, Andrew, Jacques, Nicola, Coles, Holly, Tilney, Emma, Vowell, Emma, Schuster-Bruce, Martin, Pitts, Sally, Miln, Rebecca, Purandare, Laura, Vamplew, Luke, Spivey, Michael, Bean, Sarah, Burt, Karen, Moore, Lorraine, Day, Christopher, Gibson, Charly, Gordon, Elizabeth, Zitter, Letizia, Keenan, Samantha, Baker, Evelyn, Cherian, Shiney, Cutler, Sean, Roynon-Reed, Anna, Harrington, Kate, Raithatha, Ajay, Bauchmuller, Kris, Ahmad, Norfaizan, Grecu, Irina, Trodd, Dawn, Martin, Jane, Wrey Brown, Caroline, Arias, Ana-Marie, Craven, Thomas, Hope, David, Singleton, Jo, Clark, Sarah, Rae, Nicola, Welters, Ingeborg, Hamilton, David Oliver, Williams, Karen, Waugh, Victoria, Shaw, David, Puthucheary, Zudin, Martin, Timothy, Santos, Filipa, Uddin, Ruzena, Somerville, Alastair, Tatham, Kate Colette, Jhanji, Shaman, Black, Ethel, Dela Rosa, Arnold, Howle, Ryan, Tully, Redmond, Drummond, Andrew, Dearden, Joy, Philbin, Jennifer, Munt, Sheila, Vuylsteke, Alain, Chan, Charles, Victor, Saji, Matsa, Ramprasad, Gellamucho, Minerva, Creagh-Brown, Ben, Tooley, Joe, Montague, Laura, De Beaux, Fiona, Bullman, Laetitia, Kersiake, Ian, Demetriou, Carrie, Mitchard, Sarah, Ramos, Lidia, White, Katie, Donnison, Phil, Johns, Maggie, Casey, Ruth, Mattocks, Lehentha, Salisbury, Sarah, Dark, Paul, Claxton, Andrew, McLachlan, Danielle, Slevin, Kathryn, Lee, Stephanie, Hulme, Jonathan, Joseph, Sibet, Kinney, Fiona, Senya, Ho Jan, Oborska, Aneta, Kayani, Abdul, Hadebe, Bernard, Orath Prabakaran, Rajalakshmi, Nichols, Lesley, Thomas, Matt, Worner, Ruth, Faulkner, Beverley, Gendall, Emma, Hayes, Kati, Hamilton-Davies, Colin, Chan, Carmen, Mfuko, Celina, Abbass, Hakam, Mandadapu, Vineela, Leaver, Susannah, Forton, Daniel, Patel, Kamal, Paramasivam, Elankumaran, Powell, Matthew, Gould, Richard, Wilby, Elizabeth, Howcroft, Clare, Banach, Dorota, Fernández de Pinedo Artaraz, Ziortza, Cabreros, Leilani, White, Ian, Croft, Maria, Holland, Nicky, Pereira, Rita, Zaki, Ahmed, Johnson, David, Jackson, Matthew, Garrard, Hywel, Juhaz, Vera, Roy, Alistair, Rostron, Anthony, Woods, Lindsey, Cornell, Sarah, Pillai, Suresh, Harford, Rachel, Rees, Tabitha, Ivatt, Helen, Sundara Raman, Ajay, Davey, Miriam, Lee, Kelvin, Barber, Russell, Chablani, Manish, Brohi, Farooq, Jagannathan, Vijay, Clark, Michele, Purvis, Sarah, Wetherill, Bill, Dushianthan, Ahilanandan, Cusack, Rebecca, de Courcy-Golder, Kim, Smith, Simon, Jackson, Susan, Attwood, Ben, Parsons, Penny, Page, Valerie, Zhao, Xiao Bei, Oza, Deepali, Rhodes, Jonathan, Anderson, Tom, Morris, Sheila, Xia Le Tai, Charlotte, Thomas, Amy, Keen, Alexandra, Digby, Stephen, Cowley, Nicholas, Southern, David, Reddy, Harsha, Campbell, Andy, Watkins, Claire, Smuts, Sara, Touma, Omar, Barnes, Nicky, Alexander, Peter, Felton, Tim, Ferguson, Susan, Sellers, Katharine, Bradley-Potts, Joanne, Yates, David, Birkinshaw, Isobel, Kell, Kay, Marshall, Nicola, Carr-Knott, Lisa, Writing Committee for the REMAP-CAP Investigators, Menon, David [0000-0002-3228-9692], Apollo - University of Cambridge Repository, Medical Microbiology and Infection Prevention, and AII - Infectious diseases
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Male ,Hydrocortisone ,Anti-Inflammatory Agents ,lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] ,01 natural sciences ,law.invention ,0302 clinical medicine ,Randomized controlled trial ,law ,Adrenal Cortex Hormones ,Clinical endpoint ,Medicine ,030212 general & internal medicine ,Hydrocortisone/administration & dosage ,Original Investigation ,2. Zero hunger ,Mortality rate ,Shock ,Covid19 ,General Medicine ,Middle Aged ,Intensive care unit ,3. Good health ,Intensive Care Units ,Treatment Outcome ,Early Termination of Clinical Trials ,Corticosteroid ,Female ,Coronavirus Infections ,medicine.drug ,Adult ,medicine.medical_specialty ,Respiration, Artificial/statistics & numerical data ,medicine.drug_class ,Anti-Inflammatory Agents/administration & dosage ,Pneumonia, Viral ,UNCOVER ,Adrenal Cortex Hormones/therapeutic use ,03 medical and health sciences ,Betacoronavirus ,All institutes and research themes of the Radboud University Medical Center ,Internal medicine ,Humans ,0101 mathematics ,Adverse effect ,Pandemics ,business.industry ,SARS-CoV-2 ,010102 general mathematics ,COVID-19 ,Odds ratio ,Coronavirus Infections/drug therapy ,Pneumonia, Viral/drug therapy ,Respiration, Artificial ,COVID-19 Drug Treatment ,Shock/drug therapy ,Human medicine ,business - Abstract
Importance: Evidence regarding corticosteroid use for severe coronavirus disease 2019 (COVID-19) is limited.Objective: To determine whether hydrocortisone improves outcome for patients with severe COVID-19.Design, Setting, and Participants: An ongoing adaptive platform trial testing multiple interventions within multiple therapeutic domains, for example, antiviral agents, corticosteroids, or immunoglobulin. Between March 9 and June 17, 2020, 614 adult patients with suspected or confirmed COVID-19 were enrolled and randomized within at least 1 domain following admission to an intensive care unit (ICU) for respiratory or cardiovascular organ support at 121 sites in 8 countries. Of these, 403 were randomized to open-label interventions within the corticosteroid domain. The domain was halted after results from another trial were released. Follow-up ended August 12, 2020.Interventions: The corticosteroid domain randomized participants to a fixed 7-day course of intravenous hydrocortisone (50 mg or 100 mg every 6 hours) (n = 143), a shock-dependent course (50 mg every 6 hours when shock was clinically evident) (n = 152), or no hydrocortisone (n = 108).Main Outcomes and Measures: The primary end point was organ support-free days (days alive and free of ICU-based respiratory or cardiovascular support) within 21 days, where patients who died were assigned -1 day. The primary analysis was a bayesian cumulative logistic model that included all patients enrolled with severe COVID-19, adjusting for age, sex, site, region, time, assignment to interventions within other domains, and domain and intervention eligibility. Superiority was defined as the posterior probability of an odds ratio greater than 1 (threshold for trial conclusion of superiority >99%).Results: After excluding 19 participants who withdrew consent, there were 384 patients (mean age, 60 years; 29% female) randomized to the fixed-dose (n = 137), shock-dependent (n = 146), and no (n = 101) hydrocortisone groups; 379 (99%) completed the study and were included in the analysis. The mean age for the 3 groups ranged between 59.5 and 60.4 years; most patients were male (range, 70.6%-71.5%); mean body mass index ranged between 29.7 and 30.9; and patients receiving mechanical ventilation ranged between 50.0% and 63.5%. For the fixed-dose, shock-dependent, and no hydrocortisone groups, respectively, the median organ support-free days were 0 (IQR, -1 to 15), 0 (IQR, -1 to 13), and 0 (-1 to 11) days (composed of 30%, 26%, and 33% mortality rates and 11.5, 9.5, and 6 median organ support-free days among survivors). The median adjusted odds ratio and bayesian probability of superiority were 1.43 (95% credible interval, 0.91-2.27) and 93% for fixed-dose hydrocortisone, respectively, and were 1.22 (95% credible interval, 0.76-1.94) and 80% for shock-dependent hydrocortisone compared with no hydrocortisone. Serious adverse events were reported in 4 (3%), 5 (3%), and 1 (1%) patients in the fixed-dose, shock-dependent, and no hydrocortisone groups, respectively.Conclusions and Relevance: Among patients with severe COVID-19, treatment with a 7-day fixed-dose course of hydrocortisone or shock-dependent dosing of hydrocortisone, compared with no hydrocortisone, resulted in 93% and 80% probabilities of superiority with regard to the odds of improvement in organ support-free days within 21 days. However, the trial was stopped early and no treatment strategy met prespecified criteria for statistical superiority, precluding definitive conclusions.Trial Registration: ClinicalTrials.gov Identifier: NCT02735707.
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- 2020
24. Structural genomics of highly conserved microbial genes of unknown function in search of new antibacterial targets
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Abergel, Chantal, Coutard, Bruno, Byrne, Deborah, Chenivesse, Sabine, Claude, Jean-Baptiste, Deregnaucourt, Céline, Fricaux, Thierry, Gianesini-Boutreux, Celine, Jeudy, Sandra, Lebrun, Régine, Maza, Caroline, Notredame, Cédric, Poirot, Olivier, Suhre, Karsten, Varagnol, Majorie, and Claverie, Jean-Michel
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- 2003
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25. HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacterium nostoc PCC 7120
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Xu, Xiaomei, primary, Risoul, Véronique, additional, Byrne, Deborah, additional, Champ, Stéphanie, additional, Douzi, Badreddine, additional, and Latifi, Amel, additional
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- 2020
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26. A divergent CheW confers plasticity to nucleoid-associated chemosensory arrays
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Guiseppi, Annick, primary, Vicente, Juan Jesus, additional, Herrou, Julien, additional, Byrne, Deborah, additional, Barneoud, Aurelie, additional, Moine, Audrey, additional, Espinosa, Leon, additional, Basse, Marie-Jeanne, additional, Molle, Virginie, additional, Mignot, Tâm, additional, Roche, Philippe, additional, and Mauriello, Emilia M. F., additional
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- 2019
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27. Zinc induces temperature-dependent reversible self-assembly of Tau
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Roman, Andrei, Devred, François, Byrne, Deborah, La Rocca, Romain, Ninkina, Natalia, Peyrot, Vincent, Tsvetkov, Philipp, Roman, Andrei Yu., Centre de Recherches en Oncologie biologique et Oncopharmacologie (CRO2), Aix Marseille Université (AMU)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de neurophysiopathologie (INP), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut de Microbiologie de la Méditerranée (IMM), Institute of Physiologically Active Compounds, Russian Academy of Sciences - Chernogolovka, and Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)
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Amyloid ,[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,chemistry.chemical_element ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,tau Proteins ,Zinc ,Polymerization ,03 medical and health sciences ,0302 clinical medicine ,Dynamic light scattering ,Structural Biology ,mental disorders ,Humans ,Chelation ,Binding site ,Molecular Biology ,030304 developmental biology ,Neurons ,0303 health sciences ,Binding Sites ,Heparin ,Chemistry ,Temperature ,Neurodegenerative Diseases ,Isothermal titration calorimetry ,In vitro ,[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,Biophysics ,Turbidimetry ,Tau ,Protein Processing, Post-Translational ,Aggregation self - assembly ,030217 neurology & neurosurgery ,Intracellular - Abstract
International audience; Tau is an intrinsically disordered microtubule associated protein that is implicated in several neurodegenerative disorders called Tau opathies. In these diseases, Tau is found in the form of intracellular inclusions that consist of aggregated paired helical filaments (PHFs) in neurons. Given the importance of this irreversible PHF formation in neurodegenerative disease, Tau aggregation has been extensively studied. Several different factors, such as mutations or post translational modifications, have been shown to influence the formation of late stage non-reversible Tau aggregates. It was recently shown that zinc ions accelerated heparin induced oligomerization of Tau constructs. Indeed, in vitro studies of PHFs have usually been performed in the presence of additional co-factors, such as heparin, in order to accelerate their formation. Using turbidimetry, we investigated the impact of zinc ions on Tau in the absence of heparin and found that zinc is able to induce a temperature dependent reversible oligomerization of Tau. The obtained oligomers were not amyloid like, and dissociated instantly following zinc chelation or a temperature decrease. Finally, a combination of isothermal titration calorimetry and dynamic light scattering experiments showed zinc binding to a high affinity binding site and three low affinity sites on Tau, accompanied by a change in Tau folding. Altogether, our findings stress the importance of zinc in Tau oligomerization. This newly identified Zn-induced oligomerization mechanism may be a part of a pathway different of and concurrent to Tau aggregation cascade leading to PHF formation.
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- 2019
28. Zinc binding to RNA recognition motif of TDP-43 induces the formation of amyloid-like aggregates
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Garnier, Cyrille, Devred, Francois, Byrne, Deborah, Puppo, Remy, Roman, Andrei Yu., Malesinski, Soazig, Golovin, Andrey V., Lebrun, Regine, Ninkina, Natalia N., Tsvetkov, Philipp O., Mécanismes moléculaires dans les démences neurodégénératives (MMDN), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Centre de Recherches en Oncologie biologique et Oncopharmacologie (CRO2), Aix Marseille Université (AMU)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Microbiologie de la Méditerranée (IMM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institute of Physiologically Active Compounds, Russian Academy of Sciences - Chernogolovka, Lomonosov Moscow State University (MSU), School of Biosciences [Cardiff], Cardiff University, Devred, François, École pratique des hautes études (EPHE), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)
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Amyloid ,Binding Sites ,Science ,[SDV]Life Sciences [q-bio] ,nutritional and metabolic diseases ,[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,macromolecular substances ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Article ,nervous system diseases ,DNA-Binding Proteins ,Molecular Docking Simulation ,[SDV] Life Sciences [q-bio] ,[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,Zinc ,mental disorders ,[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Medicine ,Humans ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,Protein Binding - Abstract
Aggregation of TDP-43 (transactive response DNA binding protein 43 kDa) is a hallmark of certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Moreover, intracellular TDP-43-positive inclusions are often found in other neurodegenerative diseases. Recently it was shown that zinc ions can provoke the aggregation of endogenous TDP-43 in cells, allowing to assume a direct interaction of TDP-43 with zinc ions. In this work, we investigated zinc binding to the 102–269 TDP-43 fragment, which comprise the two RNA recognition motifs. Using isothermal titration calorimetry, mass spectrometry, and differential scanning fluorimetry, we showed that zinc binds to this TDP-43 domain with a dissociation constant in the micromolar range and modifies its tertiary structure leading to a decrease of its thermostability. Moreover, the study by dynamic light scattering and negative stain electron microscopy demonstrated that zinc ions induce auto-association process of this TDP-43 fragment into rope-like structures. These structures are thioflavin-T-positive allowing to hypothesize the direct implication of zinc ions in pathological aggregation of TDP-43.
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- 2017
29. The Global Redox Responding RegB/RegA Signal Transduction System Regulates the Genes Involved in Ferrous Iron and Inorganic Sulfur Compound Oxidation of the Acidophilic Acidithiobacillus ferrooxidans
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Moinier, Danielle, Byrne, Deborah, Amouric, Agnes, Bonnefoy, Violaine, Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), and Institut de Microbiologie de la Méditerranée (IMM)
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Microbiology (medical) ,inorganic chemicals ,two-component signal transducing system ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,inorganic sulfur compound oxidation ,lcsh:QR1-502 ,Acidithiobacillus ferrooxidans ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Microbiology ,lcsh:Microbiology ,redox potential ,RegB/RegA ,[SDE]Environmental Sciences ,ferrous iron oxidation ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Original Research - Abstract
International audience; The chemical attack of ore by ferric iron and/or sulfuric acid releases valuable metals. The products of these reactions are recycled by iron and sulfur oxidizing microorganisms. These acidophilic chemolithotrophic prokaryotes, among which Acidithiobacillus ferrooxidans, grow at the expense of the energy released from the oxidation of ferrous iron and/or inorganic sulfur compounds (ISCs). In At. ferrooxidans, it has been shown that the expression of the genes encoding the proteins involved in these respiratory pathways is dependent on the electron donor and that the genes involved in iron oxidation are expressed before those responsible for ISCs oxidation when both iron and sulfur are present. Since the redox potential increases during iron oxidation but remains stable during sulfur oxidation, we have put forward the hypothesis that the global redox responding two components system RegB/RegA is involved in this regulation. To understand the mechanism of this system and its role in the regulation of the aerobic respiratory pathways in At. ferrooxidans, the binding of different forms of RegA (DNA binding domain, wild-type, unphosphorylated and phosphorylated-like forms of RegA) on the regulatory region of different genes/operons involved in ferrous iron and ISC oxidation has been analyzed. We have shown that the four RegA forms are able to bind specifically the upstream region of these genes. Interestingly, the phosphorylation of RegA did not change its affinity for its cognate DNA. The transcriptional start site of these genes/operons has been determined. In most cases, the RegA binding site(s) was (were) located upstream from the -35 (or -24) box suggesting that RegA does not interfere with the RNA polymerase binding. Based on the results presented in this report, the role of the RegB/RegA systemin the regulation of the ferrous iron and ISC oxidation pathways in At. ferrooxidans is discussed.
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- 2017
30. HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacterium nostoc PCC 7120.
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Xiaomei Xu, Risoul, Véronique, Byrne, Deborah, Champ, Stéphanie, Douzi, Badreddine, and Latifi, Amel
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- 2020
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31. Dual regulation of activity and intracellular localization of the PASTA kinase PrkC during Bacillus subtilis growth
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Pompeo, Frédérique, primary, Byrne, Deborah, additional, Mengin-Lecreulx, Dominique, additional, and Galinier, Anne, additional
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- 2018
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32. The nucleoid as a scaffold for the assembly of bacterial signaling complexes
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Moine, Audrey, primary, Espinosa, Leon, additional, Martineau, Eugenie, additional, Yaikhomba, Mutum, additional, Jazleena, P. J., additional, Byrne, Deborah, additional, Biondi, Emanuele G., additional, Notomista, Eugenio, additional, Brilli, Matteo, additional, Molle, Virginie, additional, Gayathri, Pananghat, additional, Mignot, Tâm, additional, and Mauriello, Emilia M. F., additional
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- 2017
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33. mRNA deep sequencing reveals 75 new genes and a complex transcriptional landscape in Mimivirus
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Legendre, Matthieu, Audic, Stephane, Poirot, Olivier, Hingamp, Pascal, Seltzer, Virginie, Byrne, Deborah, Lartigue, Audrey, Lescot, Magali, Bernadac, Alain, Poulain, Julie, Abergel, Chantal, and Claverie, Jean-Michel
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Nucleotide sequencing -- Usage ,Genetic transcription -- Research ,Protozoa -- Genetic aspects ,Messenger RNA -- Research ,Health - Published
- 2010
34. RAISING THE BAR.
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Byrne, Deborah
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INTELLIGENCE levels - Abstract
The article discusses the architectural design of the Ken Soble Tower in Hamilton, Ontario designed by architect ERA Architects Inc.
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- 2021
35. Glycan Dependence of Galectin-3 Self-Association Properties
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Halimi, Hubert, primary, Rigato, Annafrancesca, additional, Byrne, Deborah, additional, Ferracci, Géraldine, additional, Sebban-Kreuzer, Corinne, additional, ElAntak, Latifa, additional, and Guerlesquin, Francoise, additional
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- 2014
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36. An ArsR/SmtB Family Member Is Involved in the Regulation by Arsenic of the Arsenite Oxidase Operon in Thiomonas arsenitoxydans
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Moinier, Danielle, primary, Slyemi, Djamila, additional, Byrne, Deborah, additional, Lignon, Sabrina, additional, Lebrun, Régine, additional, Talla, Emmanuel, additional, and Bonnefoy, Violaine, additional
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- 2014
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37. Identification of a Src kinase SH3 binding site in the C‐terminal domain of the human ErbB2 receptor tyrosine kinase
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Bornet, Olivier, primary, Nouailler, Matthieu, additional, Feracci, Michaël, additional, Sebban-Kreuzer, Corinne, additional, Byrne, Deborah, additional, Halimi, Hubert, additional, Morelli, Xavier, additional, Badache, Ali, additional, and Guerlesquin, Françoise, additional
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- 2014
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38. Manual de MARC: como interpretar y usar registros MARC. Buenos Aires: GREBYD, 2001. xxv, 270 p. ISBN 987-98052-2-4
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Byrne, Deborah J.
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formato MARC ,reseñas de libros ,formatos bibliográficos - Abstract
traducido y adaptado de la 2a. ed. en inglés en GREBYD. Rucks, C. Nicolás. Reseñado por Graciela Spedalieri
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- 2001
39. The polyadenylation site of Mimivirus transcripts obeys a stringent ‘hairpin rule’
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Byrne, Deborah, primary, Grzela, Renata, additional, Lartigue, Audrey, additional, Audic, Stéphane, additional, Chenivesse, Sabine, additional, Encinas, Stéphanie, additional, Claverie, Jean-Michel, additional, and Abergel, Chantal, additional
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- 2009
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40. Mimivirus TyrRS: preliminary structural and functional characterization of the first amino-acyl tRNA synthetase found in a virus
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Abergel, Chantal, primary, Chenivesse, Sabine, additional, Byrne, Deborah, additional, Suhre, Karsten, additional, Arondel, Vincent, additional, and Claverie, Jean-Michel, additional
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- 2005
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41. Mechanism of Zn 2+ and Ca 2+ Binding to Human S100A1.
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Baksheeva, Viktoriia E., Roman, Andrei Yu., Villard, Claude, Devred, François, Byrne, Deborah, Yatoui, Dahbia, Zalevsky, Arthur O., Vologzhannikova, Alisa A., Sokolov, Andrey S., Permyakov, Sergei E., Golovin, Andrey V., Shaw, Gary S., Tsvetkov, Philipp O., and Zernii, Evgeni Yu.
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ZINC ions ,CELL differentiation ,CELLULAR control mechanisms ,CELLULAR signal transduction ,MONOMERS ,CALCIUM ions - Abstract
S100A1 is a member of the S100 family of small ubiquitous Ca
2+ -binding proteins, which participates in the regulation of cell differentiation, motility, and survival. It exists as homo- or heterodimers. S100A1 has also been shown to bind Zn2+ , but the molecular mechanisms of this binding are not yet known. In this work, using ESI-MS and ITC, we demonstrate that S100A1 can coordinate 4 zinc ions per monomer, with two high affinity (KD ~4 and 770 nm) and two low affinity sites. Using competitive binding experiments between Ca2+ and Zn2+ and QM/MM molecular modeling we conclude that Zn2+ high affinity sites are located in the EF-hand motifs of S100A1. In addition, two lower affinity sites can bind Zn2+ even when the EF-hands are saturated by Ca2+ , resulting in a 2Ca2+ :S100A1:2Zn2+ conformer. Finally, we show that, in contrast to calcium, an excess of Zn2+ produces a destabilizing effect on S100A1 structure and leads to its aggregation. We also determined a higher affinity to Ca2+ (KD ~0.16 and 24 μm) than was previously reported for S100A1, which would allow this protein to function as a Ca2+ /Zn2+ -sensor both inside and outside cells, participating in diverse signaling pathways under normal and pathological conditions. [ABSTRACT FROM AUTHOR]- Published
- 2021
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- View/download PDF
42. An enterprise directory solution with DB2.
- Author
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Shi, Shepherd S. B., Stokes, Ellen, Byrne, Deborah, Corn, Cindy F., Bachmann, David, and Jones, Tom
- Subjects
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DIRECTORIES , *COMPUTER network protocols , *COMPUTER software , *COMPUTER network resources - Abstract
LDAP (Lightweight Directory Access Protocol) is a technology that can provide directory services to a range of applications. Directory service, a critical part of distributed computing, is the central point where network services, security services, and applications can form an integrated distributed computing environment. The simplicity of LDAP enables users to store and retrieve data easily from the directory. Nevertheless, as the use of directory services becomes more widespread, directories will need to scale to support millions of entries and millions of user requests with subsecond predictable performance. LDAP directories can be implemented using various storage mechanisms such as flat files, b-trees, or databases. This paper discusses an implementation of LDAP that uses the IBM DATABASE 2 Trademark relational database as the data store and query engine to meet the directory service requirements. Performance analysis is provided to show that a relational database can be used to successfully meet the performance and scale needs of an LDAP directory while remaining secure and competitive with other vendor implementations. [ABSTRACT FROM AUTHOR]
- Published
- 2000
- Full Text
- View/download PDF
43. The nucleoid as a scaffold for the assembly of bacterial signaling complexes
- Author
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Leon Espinosa, Virginie Molle, Audrey Moine, Eugenio Notomista, Emilia M. F. Mauriello, P. Gayathri, Deborah Byrne, Tâm Mignot, Emanuele G. Biondi, Matteo Brilli, P. J. Jazleena, Eugénie Martineau, Mutum Yaikhomba, Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Indian Institute of Science Education and Research Pune (IISER Pune), Institut de Microbiologie de la Méditerranée (IMM), Dipartimento di Biologia, University of Naples Federico II = Università degli studi di Napoli Federico II, Bioinformatique, phylogénie et génomique évolutive (BPGE), Département PEGASE [LBBE] (PEGASE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), ANR-14-CE11-0023,Bacterial sensing,Perception microbiénne pendant les comportements sociaux(2014), Università degli studi di Napoli Federico II, Universita degli Studi di Padova, Dynamique des interactions membranaires normales et pathologiques (DIMNP), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Moine, Audrey, Espinosa, Leon, Martineau, Eugenie, Yaikhomba, Mutum, Jazleena, P. J., Byrne, Deborah, Biondi, Emanuele, Notomista, Eugenio, Brilli, Matteo, Molle, Virginie, Gayathri, Pananghat, Mignot, Tâm, Mauriello, Emilia M. F., Università degli Studi di Padova = University of Padua (Unipd), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon
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Cancer Research ,Scaffold ,Cytoplasm ,Cell ,Restriction Fragment Mapping ,chemistry.chemical_compound ,Cell Signaling ,Electrophoretic mobility shift assay ,Myxococcus xanthus ,Genetics (clinical) ,Staining ,0303 health sciences ,Chemotaxis ,Chemotaxi ,Myxococcus xanthu ,Specimen preparation and treatment ,Cell staining ,Cell biology ,medicine.anatomical_structure ,Cell polarity ,Chemical characterization ,Protein Binding ,Signal Transduction ,Research Article ,Cell Physiology ,animal structures ,lcsh:QH426-470 ,Bacterial Protein ,DNA binding assay ,Biology ,Research and Analysis Methods ,Central region ,03 medical and health sciences ,Electrophoretic Techniques ,Genetic ,Bacterial Proteins ,medicine ,Binding analysis ,Membrane receptor signaling ,Nucleoid ,Molecular Biology Techniques ,Molecular Biology ,Cell fusion ,030304 developmental biology ,030306 microbiology ,Gene Mapping ,fungi ,DAPI staining ,Biology and Life Sciences ,Cell Biology ,biology.organism_classification ,Gel electrophoresis ,Ecology, Evolution, Behavior and Systematic ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,lcsh:Genetics ,chemistry ,Nuclear staining ,bacteria ,DNA - Abstract
The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the nucleoid occur in a DNA-sequence independent manner and leads to the formation of multiple distributed clusters that explore constrained areas. This organization might be required for cooperative interactions between clustered receptors as observed in membrane-bound chemosensory arrays., Author summary In this work, we show that the cytoplasmic chemoreceptor of the Frz chemosensory system, FrzCD, does not bind the cytoplasmic membrane like most MCPs but bind the bacterial nucleoid directly, thus forming distributed protein clusters also containing the FrzE kinase. In vitro and in vivo experiments show that DNA-binding is not sequence-specific and is mediated by a basic aminoacid sequence of the FrzCD N-terminal domain. The deletion of this motif abolishes FrzCD DNA-binding and cooperativity in the response to signals. This work shows the importance of the nucleoid in the organization and functioning of cytoplasmic signaling systems in bacteria.
- Published
- 2017
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