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13 results on '"Calzoletti, Laura"'

1. Characterization of an influenza B virus isolated from a fatal case of myocarditis in a pediatric patient in Italy

Author

Puzelli, Simona, primary, Facchini, Marzia, additional, Piacentini, Sara, additional, Di Mario, Giuseppina, additional, Colucci, Maria Eugenia, additional, Calzoletti, Laura, additional, Fabiani, Concetta, additional, Di Martino, Angela, additional, Veronesi, Licia, additional, Biasucci, Giacomo, additional, Codeluppi, Mauro, additional, Cascio, Giuliana Lo, additional, Schiavo, Roberta, additional, Rampini, Alessandra, additional, Affanni, Paola, additional, Palamara, Anna Teresa, additional, and Stefanelli, Paola, additional

Published
2024
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2. Analysis of Genomic Characteristics of SARS-CoV-2 in Italy, 29 January to 27 March 2020

Author

Lo Presti, Alessandra, primary, Di Martino, Angela, additional, Faggioni, Giovanni, additional, Giordani, Francesco, additional, Fillo, Silvia, additional, Anselmo, Anna, additional, Fain, Vanessa Vera, additional, Fortunato, Antonella, additional, Petralito, Giancarlo, additional, Molinari, Filippo, additional, Palomba, Stefano, additional, De Santis, Riccardo, additional, Fiore, Stefano, additional, Fabiani, Concetta, additional, Di Mario, Giuseppina, additional, Facchini, Marzia, additional, Calzoletti, Laura, additional, Lista, Florigio, additional, Rezza, Giovanni, additional, and Stefanelli, Paola, additional

Published
2022
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3. Multiplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assays for Diagnostic Testing of Severe Acute Respiratory Syndrome Coronavirus 2 and Seasonal Influenza Viruses: A Challenge of the Phase 3 Pandemic Setting

Author

Mancini, Fabiola, Barbanti, Fabrizio, Scaturro, Maria, Fontana, Stefano, Di Martino, Angela, Marsili, Giulia, Puzelli, Simona, Calzoletti, Laura, Facchini, Marzia, Di Mario, Giuseppina, Fabiani, Concetta, Bella, Antonino, Riccardo, Flavia, Pezzotti, Patrizio, Stefanelli, Paola, Rezza, Giovanni, Ciervo, Alessandra, Villa, Laura, Fortini, Daniela, Iacobino, Angelo, Fiore, Stefano, Benedetti, Eleonora, Marchi, Antonella, Venturi, Giulietta, Fortuna, Claudia, Amendola, Antonello, Toma, Luciano, Di Luca, Marco, and Severini, Francesco

Subjects
biology, business.industry, Orthomyxoviridae, Context (language use), biology.organism_classification, medicine.disease_cause, Virology, Virus, law.invention, Infectious Diseases, law, Pandemic, Multiplex polymerase chain reaction, Medicine, Immunology and Allergy, Multiplex, business, Polymerase chain reaction, Coronavirus
Abstract

Background Pandemic coronavirus disease 2019 (COVID-19) disease represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters and possibly increased diffuse transmission could occur in the context of the next influenza season. For this reason, a diagnostic test able to discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza viruses is urgently needed. Methods A multiplex real-time reverse-transcription polymerase chain reaction (PCR) assay was assessed using 1 laboratory protocol with different real-time PCR instruments. Overall, 1000 clinical samples (600 from samples SARS-CoV-2–infected patients, 200 samples from influenza-infected patients, and 200 negative samples) were analyzed. Results The assay developed was able to detect and discriminate each virus target and to intercept coinfections. The limit of quantification of each assay ranged between 5 and 10 genomic copy numbers, with a cutoff value of 37.7 and 37.8 for influenza and SARS-CoV-2 viruses, respectively. Only 2 influenza coinfections were detected in COVID-19 samples. Conclusions This study suggests that multiplex assay is a rapid, valid, and accurate method for the detection of SARS-CoV-2 and influenza viruses in clinical samples. The test may be an important diagnostic tool for both diagnostic and surveillance purposes during the seasonal influenza activity period.

Published
2020
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4. Multiplex rt-Real Time PCR assays for diagnostic testing of SARS-CoV-2 and seasonal influenza viruses. A challenge of the phase 3 pandemic setting

Author

Mancini, Fabiola, Barbanti, Fabrizio, Scaturro, Maria, Fontana, Stefano, Di Martino, Angela, Marsili, Giulia, Puzelli, Simona, Calzoletti, Laura, Facchini, Marzia, Di Mario, Giuseppina, Fabiani, Concetta, DSTAT, Antonino Bella, Riccardo, Flavia, DSTAT, Patrizio Pezzotti, Stefanelli, Paola, Rezza, Giovanni, and Ciervo, Alessandra

Subjects
multiplex Real Time PCR, SARS-CoV-2, virus diseases, COVID-19, Reproducibility of Results, Orthomyxoviridae, Sensitivity and Specificity, Diagnosis, Differential, AcademicSubjects/MED00290, ROC Curve, Area Under Curve, differential diagnosis, Influenza, Human, Major Article, Humans, RNA, Viral, Seasons, influenza viruses, Multiplex Polymerase Chain Reaction
Abstract

Pandemic coronavirus disease 2019 (COVID-19) disease represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters and possibly increased diffuse transmission could occur in the context of the next influenza season. For this reason, a diagnostic test able to discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza viruses is urgently needed.A multiplex real-time reverse-transcription polymerase chain reaction (PCR) assay was assessed using 1 laboratory protocol with different real-time PCR instruments. Overall, 1000 clinical samples (600 from samples SARS-CoV-2-infected patients, 200 samples from influenza-infected patients, and 200 negative samples) were analyzed.The assay developed was able to detect and discriminate each virus target and to intercept coinfections. The limit of quantification of each assay ranged between 5 and 10 genomic copy numbers, with a cutoff value of 37.7 and 37.8 for influenza and SARS-CoV-2 viruses, respectively. Only 2 influenza coinfections were detected in COVID-19 samples.This study suggests that multiplex assay is a rapid, valid, and accurate method for the detection of SARS-CoV-2 and influenza viruses in clinical samples. The test may be an important diagnostic tool for both diagnostic and surveillance purposes during the seasonal influenza activity period.

Published
2020

5. Immunogenicity of Viral Vaccines in the Italian Military

Author

Ferlito, Claudia, primary, Biselli, Roberto, additional, Visco, Vincenzo, additional, Cattaruzza, Maria Sofia, additional, Capobianchi, Maria Rosaria, additional, Castilletti, Concetta, additional, Lapa, Daniele, additional, Nicoletti, Loredana, additional, Marchi, Antonella, additional, Magurano, Fabio, additional, Ciccaglione, Anna Rita, additional, Chionne, Paola, additional, Madonna, Elisabetta, additional, Donatelli, Isabella, additional, Calzoletti, Laura, additional, Fabiani, Concetta, additional, Biondo, Michela Ileen, additional, Teloni, Raffaela, additional, Mariotti, Sabrina, additional, Salerno, Gerardo, additional, Picchianti-Diamanti, Andrea, additional, Salemi, Simonetta, additional, Caporuscio, Sara, additional, Autore, Alberto, additional, Lulli, Patrizia, additional, Borelli, Francesco, additional, Lastilla, Marco, additional, Nisini, Roberto, additional, and D’Amelio, Raffaele, additional

Published
2021
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6. Integrase defective lentiviral vector as a vaccine platform for delivering Influenza antigens

Author

Gallinaro, Alessandra, Borghi, Martina, Bona, Roberta, Grasso, Felicia, Calzoletti, Laura, Palladino, Laura, Cecchetti, Serena, Vescio, Maria Fenicia, Macchia, Daniele, Morante, Valeria, Canitano, Andrea, Temperton, Nigel J., Castrucci, Maria Rita, Salvatore, Mirella, Michelini, Zuleika, Cara, Andrea, Negri, Donatella, Gallinaro, Alessandra, Borghi, Martina, Bona, Roberta, Grasso, Felicia, Calzoletti, Laura, Palladino, Laura, Cecchetti, Serena, Vescio, Maria Fenicia, Macchia, Daniele, Morante, Valeria, Canitano, Andrea, Temperton, Nigel J., Castrucci, Maria Rita, Salvatore, Mirella, Michelini, Zuleika, Cara, Andrea, and Negri, Donatella

Abstract

Viral vectors represent an attractive technology for vaccine delivery. We exploited the integrase defective lentiviral vector (IDLV) as a platform for delivering relevant antigens within the context of the ADITEC collaborative research program. In particular, Influenza virus hemagglutinin (HA) and nucleoprotein (NP) were delivered by IDLVs while H1N1 A/California/7/2009 subunit vaccine (HAp) with or without adjuvant was used to compare the immune response in a murine model of immunization. In order to maximize the antibody response against HA, both IDLVs were also pseudotyped with HA (IDLV-HA/HA and IDLV-NP/HA, respectively). Groups of CB6F1 mice were immunized intramuscularly with a single dose of IDLV-NP/HA, IDLV-HA/HA, HAp alone or with HAp together with the systemic adjuvant MF59. Six months after the vaccine prime all groups were boosted with HAp alone. Cellular and antibody responses to influenza antigens were measured at different time points after the immunizations. Mice immunized with HA-pseudotyped IDLVs showed similar levels of anti-H1N1 IgG over time, evaluated by ELISA, which were comparable to those induced by HAp+MF59 vaccination, but significantly higher than those induced by HAp alone. The boost with HAp alone induced an increase of antibodies in all groups, and the responses were maintained at higher levels up to 18 weeks post-boost. The antibody response was functional and persistent overtime, capable of neutralizing virus infectivity, as evaluated by hemagglutination inhibition and microneutralization assays. Moreover, since neuraminidase (NA)-expressing plasmid was included during IDLV preparation, immunization with IDLV-NP/HA and IDLV-HA/HA also induced functional anti-NA antibodies, evaluated by Enzyme-Linked Lectine Assay (ELLA). IFN?-ELISPOT showed evidence of HA-specific response in IDLV-HA/HA immunized animals and persistent NP-specific CD8+ T cell response in IDLV-NP/HA immunized mice. Taken together our results indicate that IDLV can be

Published
2018

7. Integrase Defective Lentiviral Vector as a Vaccine Platform for Delivering Influenza Antigens

Author

Gallinaro, Alessandra, primary, Borghi, Martina, additional, Bona, Roberta, additional, Grasso, Felicia, additional, Calzoletti, Laura, additional, Palladino, Laura, additional, Cecchetti, Serena, additional, Vescio, Maria Fenicia, additional, Macchia, Daniele, additional, Morante, Valeria, additional, Canitano, Andrea, additional, Temperton, Nigel, additional, Castrucci, Maria Rita, additional, Salvatore, Mirella, additional, Michelini, Zuleika, additional, Cara, Andrea, additional, and Negri, Donatella, additional

Published
2018
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8. Evidence of Cross-Reactive Immunity to 2009 Pandemic Influenza A Virus in Workers Seropositive to Swine H1N1 Influenza Viruses Circulating in Italy

Author

De Marco, Maria A., primary, Porru, Stefano, additional, Cordioli, Paolo, additional, Cesana, Bruno M., additional, Moreno, Ana, additional, Calzoletti, Laura, additional, Bonfanti, Lebana, additional, Boni, Arianna, additional, Di Carlo, Antonio Scotto, additional, Arici, Cecilia, additional, Carta, Angela, additional, Castrucci, Maria R., additional, Donatelli, Isabella, additional, Tomao, Paola, additional, Peri, Vittoria M., additional, Di Trani, Livia, additional, and Vonesch, Nicoletta, additional

Published
2013
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9. Cardiac Tamponade and Heart Failure Due to Myopericarditis as a Presentation of Infection with the Pandemic H1N1 2009 Influenza A Virus

Author

Puzelli, Simona, primary, Buonaguro, Franco M., additional, Facchini, Marzia, additional, Palmieri, Annapina, additional, Calzoletti, Laura, additional, De Marco, Maria A., additional, Arace, Pasquale, additional, de Campora, Enrico, additional, Esposito, Ciro, additional, Cassone, Antonio, additional, Rezza, Giovanni, additional, and Donatelli, Isabella, additional

Published
2010
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12. Transmission of Hemagglutinin D222G Mutant Strain of Pandemic (H1N1) 2009 Virus.

Author

Puzelli, Simona, Facchini, Marzia, Spagnolo, Domenico, De Marco, Maria A., Calzoletti, Laura, Zanetti, Alessandro, Fumagalli, Roberto, Tanzi, Maria L., Cassone, Antonio, Rezza, Giovanni, and Donatelli, Isabella

Subjects
HEMAGGLUTININ, H1N1 influenza, PANDEMICS, VIRUS diseases, INFECTIOUS disease transmission, INFLUENZA
Abstract

A pandemic (H1N1) 2009 virus strain carrying the D222G mutation was identified in a severely ill man and was transmitted to a household contact. Only mild illness developed in the contact, despite his obesity and diabetes. The isolated virus reacted fully with an antiserum against the pandemic vaccine strain. [ABSTRACT FROM AUTHOR]

Published
2010
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13. Co-circulation of the two influenza B lineages during 13 consecutive influenza surveillance seasons in Italy, 2004-2017.

Author

Puzelli, Simona, Di Martino, Angela, Facchini, Marzia, Fabiani, Concetta, Calzoletti, Laura, Di Mario, Giuseppina, Palmieri, Annapina, Affanni, Paola, Camilloni, Barbara, Chironna, Maria, D'Agaro, Pierlanfranco, Giannecchini, Simone, Pariani, Elena, Serra, Caterina, Rizzo, Caterina, Bella, Antonino, Donatelli, Isabella, Castrucci, Maria Rita, the Italian Influenza Laboratory Network, and Ansaldi, Filippo

Subjects
INFLUENZA, INFLUENZA B virus, SEASONAL influenza, INFLUENZA vaccines, VIRUS diseases, INFLUENZA prevention, INFLUENZA epidemiology, EPIDEMIOLOGY, BIOLOGICAL evolution, SEASONS, RETROSPECTIVE studies
Abstract

Background: Since 1985, two antigenically distinct lineages of influenza B viruses (Victoria-like and Yamagata-like) have circulated globally. Trivalent seasonal influenza vaccines contain two circulating influenza A strains but a single B strain and thus provide limited immunity against circulating B strains of the lineage not included in the vaccine. In this study, we describe the characteristics of influenza B viruses that caused respiratory illness in the population in Italy over 13 consecutive seasons of virological surveillance, and the match between the predominant influenza B lineage and the vaccine B lineage, in each season.Methods: From 2004 to 2017, 26,886 laboratory-confirmed influenza cases were registered in Italy, of which 18.7% were type B. Among them, the lineage of 2465 strains (49%) was retrieved or characterized in this study by a real-time RT-PCR assay and/or sequencing of the hemagglutinin (HA) gene.Results: Co-circulation of both B lineages was observed each season, although in different proportions every year. Overall, viruses of B/Victoria and B/Yamagata lineages caused 53.3 and 46.7% of influenza B infections, respectively. A higher proportion of infections with both lineages was detected in children, and there was a declining frequency of B/Victoria detections with age. A mismatch between the vaccine and the predominant influenza B lineage occurred in eight out of thirteen influenza seasons under study. Considering the seasons when B accounted for > 20% of all laboratory-confirmed influenza cases, a mismatch was observed in four out of six seasons. Phylogenetic analysis of the HA1 domain confirmed the co-circulation of both lineages and revealed a mixed circulation of distinct evolutionary viral variants, with different levels of match to the vaccine strains.Conclusions: This study contributes to the understanding of the circulation of influenza B viruses in Italy. We found a continuous co-circulation of both B lineages in the period 2004-2017, and determined that children were particularly vulnerable to Victoria-lineage influenza B virus infections. An influenza B lineage mismatch with the trivalent vaccine occurred in about two-thirds of cases. [ABSTRACT FROM AUTHOR]

Published
2019
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