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9. Decoding the spatial chromatin organization and dynamic epigenetic landscapes of macrophage cells during differentiation and immune activation

Author

Lin, Da, Xu, Weize, Hong, Ping, Wu, Chengchao, Zhang, Zhihui, Zhang, Siheng, Xing, Lingyu, Yang, Bing, Zhou, Wei, Xiao, Qin, Wang, Jinyue, Wang, Cong, He, Yu, Chen, Xi, Cao, Xiaojian, Man, Jiangwei, Reheman, Aikebaier, Wu, Xiaofeng, Hao, Xingjie, Hu, Zhe, Chen, Chunli, Cao, Zimeng, Yin, Rong, Fu, Zhen F., Zhou, Rong, Teng, Zhaowei, Li, Guoliang, and Cao, Gang

Published
2022
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10. Effect of ultrasonic surface impact on the microstructural characterization and mechanical properties of 316L austenitic stainless steel.

Author

Zhu, Jiangpei, Zhuang, Mei-Ling, Qi, Yuting, Chen, Bin, and Cao, Xiaojian

Subjects
AUSTENITIC stainless steel, ULTRASONIC effects, FATIGUE limit, STRESS fractures (Orthopedics), MARTENSITIC transformations, STAINLESS steel, FATIGUE cracks
Abstract

In the present study, effect of ultrasonic impact treatment (UIT) on the microstructural characterization and mechanical properties of 316L stainless steel (hereinafter referred to as 316L) was investigated experimentally. The fatigue fracture mechanism of 316L before and after UIT was revealed. The experimental results indicated that the martensitic grain size induced at the impact edge was about 2.00 Å. The surface modified 316L formed a gradient nanostructure and induced a martensitic phase transformation. The hardness of the surface layer of the modified 316L was twice the hardness of its matrix. The tensile strengths of 316L before and after UIT were 576 MPa and 703 MPa, respectively. The stretching stripes of 316L were more disordered after UIT. The fatigue strengths of 316L before and after UIT were 267 MPa and 327 MPa, respectively. The fatigue cracking of 316L started from the austenite grain boundaries. The fatigue fracture surface was relatively rough. The fatigue crack sources of the modified 316L came from internal inclusions. The inclusions were oxides dominated by SiO2. As the stress range increased, the crack initiation site migrated to the interior and the fatigue fracture surface became flatter. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Ruxolitinib improves the inflammatory microenvironment, restores glutamate homeostasis, and promotes functional recovery after spinal cord injury

Author

Cao, Jiang, primary, Yu, Xiao, additional, Liu, Jingcheng, additional, Fu, Jiaju, additional, Wang, Binyu, additional, Wu, Chaoqin, additional, Zhang, Sheng, additional, Chen, Hongtao, additional, Wang, Zi, additional, Xu, Yinyang, additional, Sui, Tao, additional, Chang, Jie, additional, and Cao, Xiaojian, additional

Published
2024
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13. Necroptosis pathway emerged as potential diagnosis markers in spinal cord injury.

Author

Liu, Jingcheng, Cao, Jiang, Yu, Xiao, Chang, Jie, Sui, Tao, and Cao, Xiaojian

Abstract

The present research focused on identifying necroptosis‐related differentially expressed genes (NRDEGs) in spinal cord injury (SCI) to highlight potential therapeutic and prognostic target genes in clinical SCI. Three SCI‐related datasets were downloaded, including GSE151371, GSE5296 and GSE47681. MSigDB and KEGG datasets were searched for necroptosis‐related genes (NRGs). Differentially expressed genes (DEGs) and NRGs were intersected to obtain NRDEGs. The MCC algorithm was employed to select the first 10 genes as hub genes. A protein–protein interaction (PPI) network related to NRDEGs was developed utilizing STRING. Several databases were searched to predict interactions between hub genes and miRNAs, transcription factors, potential drugs, and small molecules. Immunoassays were performed to identify DEGs using CIBERSORTx. Additionally, qRT‐PCR was carried out to verify NRDEGs in an animal model of SCI. Combined analysis of all datasets identified 15 co‐expressed DEGs and NRGs. GO and KEGG pathway analyses highlighted DEGs mostly belonged to pathways associated with necroptosis and apoptosis. Hub gene expression analysis showed high accuracy in SCI diagnosis was associated with the expression of CHMP7 and FADD. A total of two hub genes, i.e. CHMP7, FADD, were considered potential targets for SCI therapy. [ABSTRACT FROM AUTHOR]

Published
2024
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17. UVA Trajectory Prediction Model and Simulation Based on Bi-LSTM

Author

YANG Rennong, YUE Longfei, SONG Min, CAO Xiaojian, and WANG Xin

Subjects
uva, trajectory prediction, bi-lstm, recurrent neural network, autonomous collision avoidance, time series, Motor vehicles. Aeronautics. Astronautics, TL1-4050
Abstract

The traditional trajectory prediction models have the problems of large model simplification and less consideration. Combined with the characteristics of flight trajectory continuity, time series and interactivity, a trajectory prediction model based on bidirectional long short term memory(Bi-LSTM) neural network is proposed. The position, heading, pitch, roll and relative information of the intruder UAV are simultaneously used as the input of the trajectory prediction model, which is more in line with the true trajectory change law. The established Bi-LSTM based trajectory prediction model is trained with adaptive learning rate learning algorithm considering momentum and speed, and performed with simulation contrastive analysis with trajectory prediction model based on Elman neural network. The results show that, compared with the trajectory prediction model based on Elman neural network, the average absolute error of the proposed model predicted by 200 points in different directions is less than 4 m, the 3D prediction effect is better, and can perform the trajectory prediction more accurately.

Published
2020
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21. Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR

Author

Aviv, Abraham, Hunt, Steven C, Lin, Jue, Cao, Xiaojian, Kimura, Masayuki, and Blackburn, Elizabeth

Subjects
Aging, Clinical Research, Genetics, Adult, Aged, Blotting, Southern, DNA, Female, Humans, Leukocytes, Male, Middle Aged, Polymerase Chain Reaction, Reproducibility of Results, Telomere, Telomere Homeostasis, Environmental Sciences, Biological Sciences, Information and Computing Sciences, Developmental Biology
Abstract

Telomere length/DNA content has been measured in epidemiological/clinical settings with the goal of testing a host of hypotheses related to the biology of human aging, but often the conclusions of these studies have been inconsistent. These inconsistencies may stem from various reasons, including the use of different telomere length measurement techniques. Here, we report the first impartial evaluation of measurements of leukocyte telomere length by Southern blot of the terminal restriction fragments and quantitative PCR (qPCR) of telomere DNA content, expressed as the ratio of telomeric product (T)/single copy gene (S) product. Blind measurements on the same samples from 50 donors were performed in two independent laboratories on two different occasions. Both the qPCR and Southern blots displayed highly reproducible results as shown by r values > 0.9 for the correlations between results obtained by either method on two occasions. The inter-assay CV measurement for the qPCR was 6.45%, while that of the Southern blots was 1.74%. The relation between the results generated by Southern blots versus those generated by qPCR deviated from linearity. We discuss the ramifications of these findings with regard to measurements of telomere length/DNA content in epidemiological/clinical circumstances.

Published
2011

22. Neuron-derived neuropeptide Y fine-tunes the splenic immune responses

Author

Yu, Jinsong, primary, Xiao, Ke, additional, Chen, Xiaohua, additional, Deng, Lulu, additional, Zhang, Lu, additional, Li, Yue, additional, Gao, Anran, additional, Gao, Junjun, additional, Wu, Chengchao, additional, Yang, Xinmei, additional, Zhou, Qiudi, additional, Yang, Jian, additional, Bao, Chenyu, additional, Jiao, Jiaji, additional, Cheng, Sheng, additional, Guo, Zhiqing, additional, Xu, Weize, additional, Cao, Xiaojian, additional, Guo, Zheng, additional, Dai, Jinxia, additional, Hu, Ji, additional, Fu, Zhenfang, additional, and Cao, Gang, additional

Published
2023
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23. USP1/UAF1-stabilized METTL3 promotes reactive astrogliosis and improves functional recovery after spinal cord injury through m6A modification of YAP1 mRNA

Author

Ge, Xuhui, primary, Ye, Wu, additional, Zhu, Yufeng, additional, Cui, Min, additional, Zhou, Jiawen, additional, Xiao, Chenyu, additional, Jiang, Dongdong, additional, Tang, Pengyu, additional, Wang, Jiaxing, additional, Wang, Zhuanghui, additional, Ji, Chengyue, additional, Zhou, Xuhui, additional, Cao, Xiaojian, additional, Liu, Wei, additional, and Cai, Weihua, additional

Published
2023
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26. Development and Clinical Trial of a New Orthopedic Surgical Robot for Positioning and Navigation

Author

Chang, Jie, primary, Yu, Lipeng, additional, Li, Qingqing, additional, Wang, Boyao, additional, Yang, Lei, additional, Cheng, Min, additional, Wang, Feng, additional, Zhang, Long, additional, Chen, Lei, additional, Li, Kun, additional, Liang, Liang, additional, Zhou, Wei, additional, Cai, Weihua, additional, Ren, Yongxin, additional, Hu, Zhiyi, additional, Huang, Zhenfei, additional, Sui, Tao, additional, Fan, Jin, additional, Wang, Junwen, additional, Li, Bo, additional, Cao, Xiaojian, additional, and Yin, Guoyong, additional

Published
2022
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31. Neuron-derived neuropeptide Y fine-tunes the splenic immune responses

Author

Yu, Jinsong, primary, Xiao, Ke, additional, Chen, Xiaohua, additional, Deng, Lulu, additional, Zhang, Lu, additional, Li, Yue, additional, Gao, Anran, additional, Gao, Junjun, additional, Wu, Chengchao, additional, Yang, Xinmei, additional, Zhou, Qiudi, additional, Yang, Jian, additional, Bao, Chenyu, additional, Jiao, Jiaji, additional, Cheng, Sheng, additional, Guo, Zhiqing, additional, Xu, Weize, additional, Cao, Xiaojian, additional, Guo, Zheng, additional, Dai, Jinxia, additional, Hu, Ji, additional, Fu, Zhenfang, additional, and Cao, Gang, additional

Published
2022
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38. Activation of glucagon-like peptide-1 receptor in microglia attenuates neuroinflammation-induced glial scarring via rescuing Arf and Rho GAP adapter protein 3 expressions after nerve injury

Author

Qian, Zhanyang, primary, Chen, Hongtao, additional, Xia, Mingjie, additional, Chang, Jie, additional, Li, Xinyu, additional, Ye, Suhui, additional, Wu, Shunjie, additional, Jiang, Shuai, additional, Bao, Junping, additional, Wang, Binyu, additional, Kong, Renyi, additional, Zhang, Sheng, additional, Zheng, Shengnai, additional, Cao, Xiaojian, additional, and Hong, Xin, additional

Published
2022
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39. Genome-wide association identifies OBFC1 as a locus involved in human leukocyte telomere biology

Author

Levy, Daniel, Neuhausen, Susan L., Hunt, Steven C., Kimura, Masayuki, Hwang, Shih-Jen, Chen, Wei, Bis, Joshua C., Fitzpatrick, Annette L., Smith, Erin, Johnson, Andrew D., Gardner, Jeffrey P., Srinivasan, Sathanur R., Schork, Nicholas, Rotter, Jerome I., Herbig, Utz, Psaty, Bruce M., Sastrasinh, Malinee, Murray, Sarah S., Vasan, Ramachandran S., Province, Michael A., Glazer, Nicole L., Lu, Xiaobin, Cao, Xiaojian, Kronmal, Richard, Mangino, Massimo, Soranzo, Nicole, Spector, Tim D., Berenson, Gerald S., Aviv, Abraham, and Kooperberg, Charles

Published
2010

40. Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genome-wide RNA Interference Screening

Author

Rahman, Khaista, primary, Jamal, Muhammad, additional, Chen, Xi, additional, Zhou, Wei, additional, Yang, Bin, additional, Zou, Yanyan, additional, Xu, Weize, additional, Lei, Yingying, additional, Wu, Chengchao, additional, Cao, Xiaojian, additional, Tyagi, Rohit, additional, Ahsan Naeem, Muhammad, additional, Lin, Da, additional, Habib, Zeshan, additional, Peng, Nan, additional, Fu, Zhen F., additional, and Cao, Gang, additional

Published
2021
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45. miR-1297 Suppresses Osteosarcoma Proliferation and Aerobic Glycolysis by Regulating PFKFB2

Author

Pan,Xiaohui, Li,Haibo, Tan,Jingxue, Weng,Xiaokun, Zhou,Li, Weng,Yiping, and Cao,Xiaojian

Subjects
OncoTargets and Therapy
Abstract

Xiaohui Pan,1,* Haibo Li,1,* Jingxue Tan,1,* Xiaokun Weng,2 Li Zhou,1 Yiping Weng,1 Xiaojian Cao3 1Department of Orthopedics, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People’s Hospital, Changzhou, Jiangsu 213003, People’s Republic of China; 2Department of Radiotherapy, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People’s Hospital, Changzhou, Jiangsu 213003, People’s Republic of China; 3Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yiping Weng; Xiaojian Cao Email wyp2020cz@163.com; dr_cxj001@163.comBackground: MiR-1297 is reported to function as a tumor suppressor of various cancers. However, the role of miR-1297 in the development of osteosarcoma (OS) has not been elaborated. The purpose of this study was to investigate the functional effects of miR-1297 on OS progression and the underlying mechanism.Methods: The expression of protein and mRNA in OS cells was evaluated by Western blotting and quantitative real-time polymerase chain reaction. Cellular proliferation was investigated by cell counting kit-8, colony formation and apoptosis assays. Bioinformatics methods were used to predict target genes. The relationship between PFKFB2 and miR-1297 was demonstrated by dual-luciferase reporter assay. Metabolic changes in OS cells were monitored using an XF96 metabolic flux analyzer.Results: We found that miR-1297 was downregulated in OS and that lower expression of miR-1297 promoted proliferation and contributed to the Warburg effect in OS cells. Furthermore, we showed that silencing PFKFB2 inhibited proliferation and reduced aerobic glycolysis while overexpression of PFKFB2 reduced the anti-tumor function of miR-1297 in OS cells. Mechanistically, miR-1297 acted as a tumor suppressor in OS and reduced the expression of PFKFB2 by directly targeting its 3ʹUTR.Conclusion: The miR-1297/PFKFB2 axis regulated OS proliferation by controlling the Warburg effect. Our results revealed a previously undiscovered function of miR-1297 in OS, which strongly linked metabolic alterations with cancer progression. Targeting miR-1297 may become a promising therapeutic approach for OS.Keywords: osteosarcoma, miR-1297, PFKFB2, Warburg effect

Published
2020

47. Reprogramming Mycobacterium TuberculosisCRISPR System for Gene Editing and Genome-Wide RNA Interference Screening

Author

Rahman, Khaista, Jamal, Muhammad, Chen, Xi, Zhou, Wei, Yang, Bin, Zou, Yanyan, Xu, Weize, Lei, Yingying, Wu, Chengchao, Cao, Xiaojian, Tyagi, Rohit, Naeem, Muhammad Ahsan, Lin, Da, Habib, Zeshan, Peng, Nan, Fu, Zhen F., and Cao, Gang

Abstract

Mycobacterium tuberculosisis the causative agent of tuberculosis (TB), which is still the leading cause of mortality from a single infectious disease worldwide. The development of novel anti-TB drugs and vaccines is severely hampered by the complicated and time-consuming genetic manipulation techniques for M. tuberculosis.Here, we harnessed an endogenous type III-A CRISPR/Cas10 system of M. tuberculosisfor efficient gene editingand RNA interference (RNAi). This simple and easy method only needs to transform a single mini-CRISPR array plasmid, thus avoiding the introduction of exogenous protein and minimizing proteotoxicity. We demonstrated that M. tuberculosisgenes can be efficiently and specifically knocked in/out by this system as confirmed by DNA high-throughput sequencing. This system was further applied to single- and multiple-gene RNAi. Moreover, we successfully performed genome-wide RNAi screeningto identify M. tuberculosisgenes regulating in vitroand intracellular growth. This system can be extensively used for exploring the functional genomics of M. tuberculosisand facilitate the development of novel anti-TB drugs and vaccines.

Published
2022
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48. JEV Infection Induces M-MDSC Differentiation Into CD3+ Macrophages in the Brain.

Author

Zhang, Nan, Gao, Xiaochen, Zhang, Weijia, Xiong, Junyao, Cao, Xiaojian, Fu, Zhen F., and Cui, Min

Subjects
MYELOID-derived suppressor cells, JAPANESE encephalitis viruses, MACROPHAGES, JAPANESE B encephalitis, BONE marrow
Abstract

Japanese encephalitis virus (JEV) is one of the most important members of the flavivirus family. It is a typical zoonotic pathogen that has caused substantial social and economic losses worldwide. The relation between JEV-induced immunosuppression and inflammatory responses has not been thoroughly investigated. In this study, cells infiltrating the brain tissue of JEV-infected mice were mainly identified as monocytic myeloid-derived suppressor cells (M-MDSCs), which subsequently differentiated into CD3+ macrophages. Co-culture with T cells showed that both splenic M-MDSCs and brain infiltrated M-MDSCs isolated from JEV-infected mice inhibited T cell proliferation through ARG1 and iNOS. The splenectomy model revealed that JEV-induced M-MDSCs were mainly derived from bone marrow and migrated to the spleen and central nervous system (CNS). The results of the transcriptome analysis and IRF7-deficient mice indicated that the ZBP1-IRF7 signaling pathway stimulated by JEV RNA played a central role in the induction of M-MDSCs. M-MDSCs migrated into the CNS through the chemokine CCL2/N-CCL2 derived from astrocytes and brain infiltrated M-MDSCs differentiated into CD3+ macrophages through a mechanism mediated by M-CSF, IL-6 and IFN-γ in the brain microenvironment. These findings provide evidence for the mechanism that JEV regulates the differentiation of M-MDSCs and thereby exacerbates pathogenicity, which represents a potential therapeutic target for Japanese encephalitis (JE). [ABSTRACT FROM AUTHOR]

Published
2022
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