10 results on '"Chartier, Céline"'
Search Results
2. BAT with molecular allergens of Aspergillus spp.: from extract to molecules to enhance diagnosis of allergic broncho-pulmonary aspergillosis
- Author
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Michel, Moise, Sereme, Youssouf, Mankouri, Farid, Gouitaa, Marion, Gautier, Clarisse, Chartier, Céline, Blanchard, Patricia, Pinchemel, Simon, Chanez, Pascal, Mège, Jean-Louis, Ranque, Stéphane, Reynaud-Gobert, Martine, and Vitte, Joana
- Published
- 2020
- Full Text
- View/download PDF
3. Comparison of three multiplex arrays in ten patients from southern France
- Author
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Klingebiel, Caroline, Chartier, Céline, Amri, Nardjes, Pinchemel, Simon, Blanchard, Patricia, Belhocine, Wahib, Michel, Moise, and Vitte, Joana
- Published
- 2020
- Full Text
- View/download PDF
4. Evaluation of Cellular Responses for the Diagnosis of Allergic Bronchopulmonary Mycosis: A Preliminary Study in Cystic Fibrosis Patients
- Author
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Michel, Moïse, Gomez, Carine, Sereme, Youssouf, Gouitaa, Marion, Chartier, Céline, Blanchard, Patricia, Pinchemel, Simon, Cassagne, Carole, Ranque, Stephane, Mege, Jean-Louis, Reynaud-Gaubert, Martine, Vitte, Joana, Microbes évolution phylogénie et infections (MEPHI), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Hôpital Nord [CHU - APHM], Assistance Publique - Hôpitaux de Marseille (APHM), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Laboratoire de Parasitologie [Hôpital de La Timone - APHM], Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques (IP-TPT), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Service de Santé des Armées-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Service de Santé des Armées- Hôpital de la Timone [CHU - APHM] (TIMONE), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010), European Project, Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de parasitologie-mycologie [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), and Service de Santé des Armées-Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Service de Santé des Armées-Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)- Hôpital de la Timone [CHU - APHM] (TIMONE)
- Subjects
cystic fibrosis ,[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,allergic mycosis ,[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system ,basophil activation test ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,lymphocyte stimulation test ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,ComputingMilieux_MISCELLANEOUS ,cellular tests - Abstract
International audience; Background: Allergic bronchopulmonary mycosis (ABPM) is an underestimated allergic disease due to fungi. Most reported cases are caused by Aspergillus fumigatus (Af) and are referred to as allergic bronchopulmonary aspergillosis (ABPA). The main risk factor of ABPA is a history of lung disease, such as cystic fibrosis, asthma, or chronic obstructive pulmonary disease. The main diagnostic criteria for ABPA rely on the evaluation of humoral IgE and IgG responses to Af extracts, although these cannot discriminate Af sensitization and ABPA. Moreover, fungi other than Af have been incriminated. Flow cytometric evaluation of functional responses of basophils and lymphocytes in the context of allergic diseases is gaining momentum. Objectives: We hypothesized that the detection of functional responses through basophil and lymphocyte activation tests might be useful for ABPM diagnosis. We present here the results of a pilot study comparing the performance of these cellular assays vs. usual diagnostic criteria in a cystic fibrosis (CF) cohort. Methods: Ex vivo basophil activation test (BAT) is a diagnostic tool highlighting an immediate hypersensitivity mechanism against an allergen, e.g., through CD63 upregulation as an indirect measure of degranulation. Lymphocyte stimulation test (LST) relies on the upregulation of activation markers, such as CD69, after incubation with allergen(s), to explain delayed hypersensitivity. These assays were performed with Af, Penicillium, and Alternaria extracts in 29 adult CF patients. Results: BAT responses of ABPA patients were higher than those of sensitized or control CF patients. The highest LST result was for a woman who developed ABPA 3 months after the tests, despite the absence of specific IgG and IgE to Af at the time of the initial investigation. Michel et al. Cellular Responses of ABPM Conclusion: We conclude that basophil and lymphocyte activation tests could enhance the diagnosis of allergic mycosis, compared to usual humoral markers. Further studies with larger cohorts and addressing both mold extracts and mold relevant molecules are needed in order to confirm and extend the application of this personalized medicine approach.
- Published
- 2020
5. Defective Granuloma Formation in Elderly Infected Patients
- Author
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Daumas, Aurélie, primary, Coiffard, Benjamin, additional, Chartier, Céline, additional, Ben amara, Amira, additional, Alingrin, Julie, additional, Villani, Patrick, additional, and Mege, Jean-Louis, additional
- Published
- 2020
- Full Text
- View/download PDF
6. Full-Term Human Placental Macrophages Eliminate Coxiella burnetii Through an IFN-γ Autocrine Loop
- Author
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Mezouar, Soraya, primary, Benammar, Imene, additional, Boumaza, Asma, additional, Diallo, Aïssatou Bailo, additional, Chartier, Céline, additional, Buffat, Christophe, additional, Boudjarane, John, additional, Halfon, Philippe, additional, Katsogiannou, Maria, additional, and Mege, Jean-Louis, additional
- Published
- 2019
- Full Text
- View/download PDF
7. High Concentrations of Serum Soluble E-Cadherin in Patients With Q Fever
- Author
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Mezouar, Soraya, Omar Osman, Ikram, Melenotte, Clea, Slimani, Camélia, Chartier, Céline, Raoult, Didier, Mege, Jean-Louis, Devaux, Christian, Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Assistance Publique-Hôpitaux de Marseille (AP-HM), Fondation pour la Recherche Médicale postdoctoral fellowship (reference: SPF20151234951) and then by the Fondation Méditerranée Infection, ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010), and Assistance Publique - Hôpitaux de Marseille (APHM)
- Subjects
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,soluble E-cadherin ,biomarker ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Q fever ,adhesion molecule ,coxiella burnetii - Abstract
International audience; Cadherins switching is a hallmark of neoplasic processes. The E-cadherin (E-cad) subtype is one of the surface molecules regulating cell-to-cell adhesion. After its cleavage by sheddases, a soluble fragment (sE-cad) is released that has been identified as a pro-carcinogenic inflammatory signal in several bacteria-induced cancers. Recently we reported that Q fever, a disease due to Coxiella burnetii infection, can be complicated by occurrence of non-Hodgkin lymphoma (NHL). Therefore, we studied E-cad switching in Q fever. The sE-cad levels were found increased in the sera of acute and persistent Q fever patients, whereas they remained at the baseline in controls groups of healthy donors, people cured of Q fever, patients suffering from unrelated inflammatory diseases, and past Q fever patients who developed NHL. These results indicate that sE-cad can be considered as a new biomarker of C. burnetii infection rather than a marker of NHL-associated to Q fever. We wondered if changes in sE-cad reflected variations in the CDH1 gene transcription. The expression of E-cad mRNA and its intracellular ligand β-catenin was down-regulated in peripheral blood mononuclear cells (PBMCs) of patients with either acute or persistent forms of Q fever. Indeed, a lower cell-surface expression of E-cad was measured in a minority (
- Published
- 2019
8. An activin receptor IIA ligand trap corrects ineffective erythropoiesis in β-thalassemia
- Author
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Dussiot, Michael, primary, Maciel, Thiago T, additional, Fricot, Aurélie, additional, Chartier, Céline, additional, Negre, Olivier, additional, Veiga, Joel, additional, Grapton, Damien, additional, Paubelle, Etienne, additional, Payen, Emmanuel, additional, Beuzard, Yves, additional, Leboulch, Philippe, additional, Ribeil, Jean-Antoine, additional, Arlet, Jean-Benoit, additional, Coté, Francine, additional, Courtois, Geneviève, additional, Ginzburg, Yelena Z, additional, Daniel, Thomas O, additional, Chopra, Rajesh, additional, Sung, Victoria, additional, Hermine, Olivier, additional, and Moura, Ivan C, additional
- Published
- 2014
- Full Text
- View/download PDF
9. Evaluation of Cellular Responses for the Diagnosis of Allergic Bronchopulmonary Mycosis: A Preliminary Study in Cystic Fibrosis Patients.
- Author
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Michel M, Gomez C, Sereme Y, Gouitaa M, Chartier C, Blanchard P, Pinchemel S, Cassagne C, Ranque S, Mège JL, Reynaud-Gaubert M, and Vitte J
- Subjects
- Adolescent, Adult, Female, Humans, Invasive Pulmonary Aspergillosis complications, Male, Middle Aged, Pilot Projects, Young Adult, Antigens, Fungal immunology, Basophil Degranulation Test methods, Cystic Fibrosis complications, Invasive Pulmonary Aspergillosis diagnosis, Lymphocyte Activation immunology
- Abstract
Background: Allergic bronchopulmonary mycosis (ABPM) is an underestimated allergic disease due to fungi. Most reported cases are caused by Aspergillus fumigatus (Af) and are referred to as allergic bronchopulmonary aspergillosis (ABPA). The main risk factor of ABPA is a history of lung disease, such as cystic fibrosis, asthma, or chronic obstructive pulmonary disease. The main diagnostic criteria for ABPA rely on the evaluation of humoral IgE and IgG responses to Af extracts, although these cannot discriminate Af sensitization and ABPA. Moreover, fungi other than Af have been incriminated. Flow cytometric evaluation of functional responses of basophils and lymphocytes in the context of allergic diseases is gaining momentum. Objectives: We hypothesized that the detection of functional responses through basophil and lymphocyte activation tests might be useful for ABPM diagnosis. We present here the results of a pilot study comparing the performance of these cellular assays vs. usual diagnostic criteria in a cystic fibrosis (CF) cohort. Methods: Ex vivo basophil activation test (BAT) is a diagnostic tool highlighting an immediate hypersensitivity mechanism against an allergen, e.g., through CD63 upregulation as an indirect measure of degranulation. Lymphocyte stimulation test (LST) relies on the upregulation of activation markers, such as CD69, after incubation with allergen(s), to explain delayed hypersensitivity. These assays were performed with Af, Penicillium , and Alternaria extracts in 29 adult CF patients. Results: BAT responses of ABPA patients were higher than those of sensitized or control CF patients. The highest LST result was for a woman who developed ABPA 3 months after the tests, despite the absence of specific IgG and IgE to Af at the time of the initial investigation. Conclusion: We conclude that basophil and lymphocyte activation tests could enhance the diagnosis of allergic mycosis, compared to usual humoral markers. Further studies with larger cohorts and addressing both mold extracts and mold relevant molecules are needed in order to confirm and extend the application of this personalized medicine approach., (Copyright © 2020 Michel, Gomez, Sereme, Gouitaa, Chartier, Blanchard, Pinchemel, Cassagne, Ranque, Mège, Reynaud-Gaubert and Vitte.)
- Published
- 2020
- Full Text
- View/download PDF
10. High Concentrations of Serum Soluble E-Cadherin in Patients With Q Fever.
- Author
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Mezouar S, Omar Osman I, Melenotte C, Slimani C, Chartier C, Raoult D, Mege JL, and Devaux CA
- Subjects
- Adult, Aged, Antigens, CD genetics, Antigens, CD metabolism, Cadherins genetics, Cadherins metabolism, Female, Gene Expression, Humans, Leukocytes, Mononuclear metabolism, Male, Middle Aged, Monocytes metabolism, Q Fever genetics, beta Catenin metabolism, Biomarkers blood, Cadherins blood, Coxiella burnetii pathogenicity, Q Fever blood
- Abstract
Cadherins switching is a hallmark of neoplasic processes. The E-cadherin (E-cad) subtype is one of the surface molecules regulating cell-to-cell adhesion. After its cleavage by sheddases, a soluble fragment (sE-cad) is released that has been identified as a pro-carcinogenic inflammatory signal in several bacteria-induced cancers. Recently we reported that Q fever, a disease due to Coxiella burnetii infection, can be complicated by occurrence of non-Hodgkin lymphoma (NHL). Therefore, we studied E-cad switching in Q fever. The sE-cad levels were found increased in the sera of acute and persistent Q fever patients, whereas they remained at the baseline in controls groups of healthy donors, people cured of Q fever, patients suffering from unrelated inflammatory diseases, and past Q fever patients who developed NHL. These results indicate that sE-cad can be considered as a new biomarker of C. burnetii infection rather than a marker of NHL-associated to Q fever. We wondered if changes in sE-cad reflected variations in the CDH1 gene transcription. The expression of E-cad mRNA and its intracellular ligand β-catenin was down-regulated in peripheral blood mononuclear cells (PBMCs) of patients with either acute or persistent forms of Q fever. Indeed, a lower cell-surface expression of E-cad was measured in a minority (<5%) subpopulation of HLADR
+ /CD16+ monocytes from patients with acute Q fever. However, a very strong increase in E-cad expression was observed on more than 30% of the HLADR+ /CD16+ monocytes of persistent Q fever patients, a cell subpopulation known to be a target for C. burnetii in humans. An experimental in vitro infection of healthy donors' PBMCs with C. burnetii , was performed to directly evaluate the link between C. burnetii interaction with PBMCs and their E-cad expression. A significant increase in the percentage of HLADR+ /CD16+ monocytes expressing E-cad was measured after PBMCs had been incubated for 8 h with C. burnetii Nine Mile strain. Altogether, these data demonstrate that C. burnetii severely impairs the E-cad expression in circulating cells of Q fever patients.- Published
- 2019
- Full Text
- View/download PDF
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