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Marine resources are rich and contain an enormous amount of energy. The exploration of marine resources and the effective use of ocean energy have gradually become the research focus of scholars all over the world. A profiler driven by ocean thermal energy can monitor the vertical profile of the surrounding sea area for a long time. To realize the levitation at a fixed water depth on the premise of saving energy, in this paper, a new buoyancy regulation system driven by the mixture of ocean thermal energy and electric energy is designed, and a new depth control strategy for the hybrid drive is proposed. Compared with the traditional profiler, the new profiler, in which the main energy required for buoyancy regulation is provided by ocean thermal energy, can reduce electrical energy consumption. Simulations of SMC (sliding mode control) and conventional PID control were conducted, and the results showed that the SMC method has advantages in terms of response speed, overshoot, and energy saving. A lake test was conducted and the results showed that the new control method can make the equipment reach the fixed water depth position; however, due to the complex water flow environment, the precision and stability of the controller need to be improved in the future. [ABSTRACT FROM AUTHOR]