Chen,Zili, Ma,Yifei, Pan,Yaozhen, Zuo,Shi, Zhu,Haitao, Yu,Chao, Zhu,Changhao, and Sun,Chengyi
Zili Chen,1,* Yifei Ma,2,* Yaozhen Pan,1 Shi Zuo,1 Haitao Zhu,1 Chao Yu,1 Changhao Zhu,1 Chengyi Sun1 1Department of Hepatobiliary Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, People’s Republic of China; 2Department of Otorhinolaryngology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chengyi SunDepartment of Hepatobiliary Surgery, Affiliated Hospital of Guizhou Medical University, No. Guiyi Street, Yunyan District, Guiyang, Guizhou 550004, People’s Republic of ChinaEmail chenyisun004@126.comBackground: Previous studies indicated that long noncoding RNAs (lncRNAs) played vital roles in the development and progression of hepatocellular carcinoma (HCC). Recently, downregulation of lncRNA RP5â833A20.1 has been observed in HCC tissues. However, the underlying mechanism by which RP5â833A20.1 regulates the proliferation and apoptosis in HCC has not been investigated. Thus, this study aimed to investigate the role of RP5â833A20.1 in the progression of HCC.Methods: The levels of RP5â833A20.1 in 30 pairs of HCC tissues and adjacent normal tissues were detected by RT-qPCR. In addition, the effects of RP5â833A20.1 on cell proliferation, apoptosis and invasion were evaluated by CCK-8, flow cytometric, transwell assays, respectively. Meanwhile, the dual-luciferase reporter system assay was used to explore the interaction of RP5â833A20.1 and miR-18a-5p in HCC.Results: The level of RP5â833A20.1 was significantly downregulated in HCC tissues and HCC cell lines. Downregulation of RP5â833A20.1 markedly promoted the proliferation and invasion of Bel-7402 cells. In addition, overexpression of RP5â833A20.1 notably inhibited the proliferation and invasion of Huh7 cells. Moreover, overexpression of RP5â833A20.1 obviously induced the apoptosis of Huh7 cells via increasing the levels of Bax and active caspase 3, and decreasing the levels of Bcl-2, p-Akt and p-ERK. Meanwhile, in vivo experiments performed also indicated that overexpression of RP5-833A20.1 could inhibit the tumorigenesis of subcutaneous Huh7 xenograft in nude mice. Furthermore, bioinformatics and luciferase reporter assay identified that RP5-833A20.1 functioned as a competing endogenous RNA (ceRNA) for miR-18a-5p in HCC.Conclusion: In this study, we found that RP5â833A20.1 was downregulated in HCC tissues. In addition, RP5-833A20.1 could suppress the tumorigenesis in HCC through inhibiting Akt/ERK pathway by acting as a ceRNA for miR-18a-5p. Therefore, RP5-833A20.1 might be a valuable and potential biomarker and therapeutic target for the treatment of HCC.Keywords: hepatocellular carcinoma, long noncoding RNA RP5â833A20.1, microRNA-18a-5p, proliferation