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Citywide inclusive sanitation (CWIS) is becoming the dominant paradigm for achieving safe sanitation for all by 2030. Its technical benefits have been explored, but the bargaining over financial and organizational changes CWIS entails have not yet been adequately addressed. Our case study explains the stalled rollout of CWIS in Dhaka, Bangladesh. We analyse policy pathways over the past 30 years through a combined territorial political economy and power perspective to understand their effects on equality. We highlight how donors link the introduction of CWIS to the organization of sanitation through a market; how the utility uses CWIS as an opportunity to avoid costly responsibilities in non-sewered sanitation; and how service co-production through community-based solutions is neglected. CWIS has successfully overcome the dogmatic technological focus in the sanitation system, but for citywide sanitation to be scaled inclusively, the dogmatic focus in the organization and financing of the sanitation sector must also be overcome.

In recent years, shared facilities have contributed substantially to increased access to sanitation in urban areas. While shared sanitation is often the only viable option in densely-populated, low-income urban areas, it is currently considered a “limited" solution by the international community. In this paper, we analyze the conditions under which shared sanitation could be considered of adequate quality and propose a set of indicators associated with sanitation quality to be included in national household surveys. We conducted a survey with 3600 households and 2026 observational spot-checks of shared and individual household toilets in Kisumu (Kenya), Kumasi (Ghana), and Dhaka (Bangladesh). We develop a composite sanitation quality outcome measure based on observational data. Using regression analysis, we identify self-reported indicators that correlate with the spot-checked composite measure and are, therefore, robust with regard to reporting bias. Results show that (pour-) flush toilets are a highly informative indicator for sanitation quality compared to other toilet technologies. In contrast to previous arguments and depending on the context, sharing a toilet has a comparatively lower correlation with sanitation quality. Toilets still show good quality if shared among only 2–3 households. Toilet location and lighting, as well as the presence of a lockable door, are equally strong indicators for sanitation quality and could serve as alternative indicators. The findings suggest that the sanitation service levels defined by the WHO and UNICEF might be reconsidered to better capture the quality of sanitation facilities in low-income urban settlements., Social Indicators Research, 162 (2), ISSN:0303-8300, ISSN:1573-0921

The water, sanitation, and solid waste sectors are closely related and have many interactions between their respective service chains in low- and middle-income countries. Currently, these interactions mostly lead to cross-contamination, and opportunities for co-benefits are seldom realized. This review presents the key advancements within each of these three development sectors in the past two decades. We identify numerous similarities such as decentralization, resource recovery, community involved planning, and digitalization. Despite the potential for synergies and the opportunities to maximize positive interactions, there have been few attempts to break the existing sectoral silos in order to integrate these three service chains. We argue that, with the right enabling environment, an integrated approachto holistically planning and implementing water supply, sanitation, and solid waste management can create positive interactions resulting in co-benefits among complementary development goals.

Sanitation is a universal need and thus requires universal access. Despite having the world's largest sanitation campaign, only 37% of urban India has access to safely managed sanitation systems. Due to the heterogeneity of Indian cities, a mix of different sanitation systems is required to meet the demands of these rapidly urbanising cities. The Manila principles on Citywide Inclusive Sanitation (CWIS) propose a mix of technologies as a key pillar in achieving the Sustainable Development Goals on urban sanitation. However, there is a lack of understanding on the criteria to consider when planning the technological options for CWIS. The mega-city of Chennai is chosen as a case study, and mixed methods including a Faecal Waste Flow diagram (SFD), Social Network Analysis (SNA) and interviews are applied. This study then presents a set of 14 contextually relevant criteria for the implementation of sewered and non-sewered technologies in the city. The SFD shows that 35% of Chennai's faecal waste remains unsafely managed while using SNA identified that the water and sanitation utility, the municipal corporation and the slum clearance board are the best placed stakeholders in the city to create a change in the sanitation situation. Furthermore, 17 drivers and barriers for the implementation of non-sewered technologies are identified. The criteria catalogue was also theoretically tested for an example ward in Chennai. The findings of the study show that a catalogue of physical, technical, institutional and financial criteria is useful for planning a mix of technologies with the CWIS approach., Environment and Planning B: Urban Analytics and City Science, 49 (8), ISSN:2399-8083, ISSN:2399-8091

The water, sanitation and hygiene (WASH) sector is facing a shortfall of several million appropriately skilled professionals. Massive open online courses (MOOCs) can play a crucial role in addressing this. This paper presents the case study of the MOOC series “Sanitation, Water and Solid Waste for Development”, which has reached over 120,000 learners within six years. It has attracted mainly well-educated, employed learners, under 34 years old, from Asia, Latin America and Africa. Underrepresentation of female learners remains a challenge. While MOOCs have proven excellent for delivering WASH education at scale, some alternative formats (e.g. blended learning, small private online courses) allow more collaborative, interactive learning environments. Three practical examples from Nigeria, Indonesia and Mozambique indicate the potential for synergies among MOOCs and further learning formats. With the global shift towards digital learning due to the COVID-19 pandemic, MOOCs have gained further traction.

Shared sanitation facilities (SSFs) have contributed considerably to sanitation access in many low-income settlements. While many SSFs are of unacceptable quality, others have been found to be a hygienically safe and a socially and economically viable sanitation option. Within its framework, the WHO/UNICEF Joint Monitoring Programme (JMP), evaluating progress on the Sustainable Development Goals, considers shared sanitation as 'limited sanitation'. Overall, there is uncertainty about the criteria to distinguish between unacceptable and acceptable quality of SSF. In our study, we used a user-centred qualitative approach in low-income urban settlements in Kumasi (Ghana), Kisumu (Kenya) and Dhaka (Bangladesh) and conducted 17 focus group discussions to evaluate how SSF users define the quality of an SSF and which aspects they consider as essential priorities for good-quality SSF. In descending order, the user priorities identified are: immediate water access, cleanliness, gender-separated toilets, flush toilets, lighting for use at night, lockable/functional doors, tiling, handwashing stations and privacy. This list can serve as input to the sanitation guidelines, local building codes and the establishment of minimum national sanitation standards. SSFs that meet these minimal criteria can then be promoted as an incremental step when individual household facilities are not feasible., Journal of Water, Sanitation and Hygiene for Development, 10 (4), ISSN:2043-9083

Gene drive organisms (GDOs) have been suggested as approaches to combat some of the most pressing environmental and public health issues. No such organisms have so far been released into the environment, but it remains unclear whether the relevant regulatory provisions will be fit for purpose to cover their potential environmental, human and animal health risks if environmental releases of GDOs are envisaged. We evaluate the novel features of GDOs and outline the resulting challenges for the environmental risk assessment. These are related to the definition of the receiving environment, the use of the comparative approach, the definition of potential harm, the stepwise testing approach, the assessment of long-term and large-scale risks at population and ecosystem level and the post-release monitoring of adverse effects. Fundamental adaptations as well as the development of adequate risk assessment methodologies are needed in order to enable an operational risk assessment for globally spreading GDOs before these organisms are released into environments in the EU.

Recent developments in high- and middle-income countries have exhibited a shift from conventional urban water systems to alternative solutions that are more diverse in source separation, decentralization, and modularization. These solutions include nongrid, small-grid, and hybrid systems to address such pressing global challenges as climate change, eutrophication, and rapid urbanization. They close loops, recover valuable resources, and adapt quickly to changing boundary conditions such as population size. Moving to such alternative solutions requires both technical and social innovations to coevolve over time into integrated socio-technical urban water systems. Current implementations of alternative systems in high- and middle-income countries are promising, but they also underline the need for research questions to be addressed from technical, social, and transformative perspectives. Future research should pursue a transdisciplinary research approach to generating evidence through socio-technical "lighthouse" projects that apply alternative urban water systems at scale. Such research should leverage experiences from these projects in diverse socio-economic contexts, identify their potentials and limitations from an integrated perspective, and share their successes and failures across the urban water sector.

The QUISS project identifies the key criteria of ‘acceptable quality’ shared toilets in low-income urban contexts and provides recommendations for strengthening the acceptability, functionality and sustainability of shared sanitation facilities.

A majority of the world's population use onsite sanitation systems, which store or treat excreta close to where it is generated. Sludge from these systems needs to be managed through a series of stages, known as the sanitation value chain. There is a huge diversity of service providers, not only within each part of the chain, but also along the chain bridging the different components. These service providers are linked not only by the flow of materials, but also by the transfer of money. Therefore for this system to be considered financially sustainable all services from the toilet to reuse or disposal need to be considered. A tool has been developed (eSOSView™) to simulate, evaluate, and optimise the financial flows along and within the sanitation value chain. In this paper eSOSView™ was tested, validated (using existing data), and piloted (including data collection). This paper demonstrates how eSOSView ™ can be used to evaluate different financial flow models, to assess financial sustainability in different parts of the sanitation value chain and optimise the financial sustainability along the sanitation value chain.

To provide access to sustainable sanitation for the entire world population, novel technologies and systems have been developed. These options are often independent of sewers, water, and energy and therefore promise to be more appropriate for fast-growing urban areas. They also allow for resource recovery and and are adaptable to changing environmental and demographic conditions what makes them more sustainable. More options, however, also enhance planning complexity. Structured decision making (SDM) can help balance opposing interests. Yet, most of the current research focuses on the selection of a preferred option, assuming that a set of appropriate options is available. There is a lack of reproducible methods for the identification of sanitation system planning options that can consider the growing number of available technology and the many possible system configurations. Additionally, there is a lack of data, particularly for novel options, to evaluate the various sustainability criteria for sanitation.To overcome this limitation, we present a novel software supported approach: the SANitation sysTem Alternative GeneratOr (Santiago). To be optimally effective, Santiago is required to be integrated into an SDM approach. In this paper, we present all the elements that such an integration requires and illustrate these methods at the case of Arba Minch, a fast growing town in Ethiopia. Based on this example and experiences from other cases, we discuss the lessons learnt and present the advantages potentially brought by Santiago for sanitation planning The integration requires four elements: a set of technologies to be looked at, decision objectives for sustainable sanitation, screening criteria to evalute technology appropriateness, and about the technologies and the casea. The main output is a set of sanitation system options that is locally appropriate, diverse in order to reveal trade-offs, and of a manageable size. To support the definition of decision objectives, we developed a generic objective hierarchy for sustainable sanitation. Because one of the main challenges lies in the quantification of screening criteria, we established the data for 27 criteria and 41 technologies in a library.The case studies showed, that if the integration is successful, then Santiago can provide substantial benefits: (i) it is systematic and reproducible; (ii) it opens up the decision space with novel and potentially more appropriate solutions; (iii) it makes international data accessible for more empirical decision making; (iv) it enables decisions based on strategic objectives in line with the sustainable development goals; (v) it allows to prioritise appropriate and resource efficient systems right from the beginning (vi) and it contributes to a more citywide inclusive approach by bridging strategic objectives with an area-based appropriateness assessment. The here presented approach enables the prioritisation of appropriate and resource efficient sanitation technologies and systems in strategic planning. Thereby this approach contributes to SDG 6.2, 6.3, and 11, sustainable sanitation for all., Journal of Environmental Management, 271, ISSN:0301-4797, ISSN:1095-8630

NoMix toilets separate urine and feces at the source and are a promising resource recovery technology. However, design issues hamper the transformation from unattractive to aspirational products. Little effort has been done to design toilets that account for physiological differences, leading to adverse effects on user-friendliness and urine separation efficiency. We used infrared recordings to assess gender-specific urination practices. Based on field data, we developed the Urinator, a simple device that allows simulating male and female urine streams. This supports engineers in developing more user- and gender-friendly and reuse-oriented sanitation technologies.

The need for taking a comprehensive perspective when selecting sanitation technologies in developing contexts has been increasingly discussed. Process guides, which are planning documents describing steps decision-makers need to take, represent one possible contribution to attain comprehensive decisions. An interview study with sanitation experts was carried out to understand the importance and real use of such planning documents for the selection of urban sanitation technologies in developing countries, as well as to understand the relevance and actual consideration of decision elements and to identify recommendations for taking them into account. Although process guides appear to be helpful to guide planning processes, their use does not seem to be common practice. It is actually doubtful that the sector is currently able to make better use of those documents in the form that they exist today. Furthermore, the importance of a comprehensive approach is generally recognised, but relevant decision elements were said to be often neglected. Finally, results from the interview analysis also emphasised the need for a conducive policy environment, namely by developing appropriate institutional and legal frameworks, and by incentivising planners and decision-makers to further adapt to comprehensive decision-making practices that effectively improve the sanitation situation in developing countries.

To achieve citywide inclusive sanitation in developing countries, a strategic sanitation planning approach (SSA) needs to provide a variety of technical solutions that respond to different urban realities. Despite the development of various SSA frameworks, sanitation planning still often follows a ‘one-size-fits-all’ approach. Structured decision making (SDM) can help by balancing trade-offs among different solutions. But SDM requires a set of appropriate sanitation options to choose from. Because conventional sewer-based sanitation is often inappropriate, many novel technologies and systems have been developed (e.g. container-based sanitation). While these innovations enhance sustainability, they also increase planning complexity. In this review, we look at available frameworks and tools for SSA and discover a lack of systematic tools for the identification of planning options that are able to consider the growing portfolio of available solutions and multiple sustainability criteria. Therefore, we critically compare 15 tools from which we compile eight qualities that could help any future tool address the current sanitation challenge: it should be comprehensive, automated to deal with a large number of options, systematic, flexible towards future innovation and should consider all sustainability dimensions, make a contextualized evaluation, allow for participation, and consider uncertainties to be applicable ex-ante also for novel technologies., Journal of Water, Sanitation and Hygiene for Development, 10 (4), ISSN:2043-9083

An intensely debated question is whether or how a mandatory environmental risk assessment (ERA) should be conducted for plants obtained through novel genomic techniques, including genome editing (GE). Some countries have already exempted certain types of GE applications from their regulations addressing genetically modified organisms (GMOs). In the European Union, the European Court of Justice confirmed in 2018 that plants developed by novel genomic techniques for directed mutagenesis are regulated as GMOs. Thus, they have to undergo an ERA prior to deliberate release or being placed on the market. Recently, the European Food Safety Authority (EFSA) published two opinions on the relevance of the current EU ERA framework for GM plants obtained through novel genomic techniques (NGTs). Regarding GE plants, the opinions confirmed that the existing ERA framework is suitable in general and that the current ERA requirements need to be applied in a case specific manner. Since EFSA did not provide further guidance, this review addresses a couple of issues relevant for the case-specific assessment of GE plants. We discuss the suitability of general denominators of risk/safety and address characteristics of GE plants which require particular assessment approaches. We suggest integrating the following two sets of considerations into the ERA: considerations related to the traits developed by GE and considerations addressing the assessment of method-related unintended effects, e.g., due to off-target modifications. In conclusion, we recommend that further specific guidance for the ERA and monitoring should be developed to facilitate a focused assessment approach for GE plants.

Planning for urban sanitation in developing contexts is one of the major development challenges of this century. Particularly, the relevance of a broad perspective in sanitation decision-making processes has been increasingly discussed. One possible contribution to achieve comprehensive decisions is through the use of planning guidelines, also called process guides. The present work first identified categories of relevant decision elements, namely: (a) the multi-sectoral approach, (b) the multiplicity of sustainable dimensions, (c) the system analysis perspective and (d) the co-existence of planning scales. Then, for each category, a comparative analysis of urban sanitation process guides was conducted with a focus on technology decision-making. It was found that the importance of considering those categories is increasingly reflected in process guides, although not always in a detailed supportive way. Recommendations are provided to increase the added value of process guides regarding technology selection, as well as, to better integrate them into urban planning.

The improvement of sanitation conditions in slums(1)is difficult in part because of economic and institutional environments that often prevent private or public investment in infrastructure. This analysis of sanitation conditions in informal settlements in Nairobi and Kampala compares user practices. It also identifies the main actors involved in the provision of overlapping sanitation systems, involving a multitude of small-scale providers, along with the challenges these present. The paper goes on to describe a new market-based approach in Nairobi, developed by a social enterprise, Sanergy, which is responsible for the construction of facilities and the collection and treatment of wastes. The system improves user satisfaction, community wellbeing and environmental quality, pointing to a clear opportunity for such market-based interventions where a customer base already pays for sub-standard services. There remain challenges, however, around the ability to scale up and finance these providers, especially in complex operating environments that increase transactional costs for companies.

This paper provides a comparative assessment of community-scale decentralized wastewater treatment systems (DEWATS) in Nepal, based on three communities in urban and peri-urban locations. The analysis provides lessons for sustainable and future replications of this approach, highlighting the benefits, drivers for long-term operation and maintenance, and barriers associated with up-scaling. Nepal is experiencing the fastest rate of urbanization in the South Asian region, while sanitation and wastewater management are emerging as some of the biggest challenges in urban areas. Over 43 per cent of the population still lacks access to a toilet, and virtually all wastewater and septage is discharged into water bodies without treatment. These mounting problems of urban sanitation demand urgent and appropriate solutions. The inherent simplicity of DEWATS provides an effective, affordable, and low-maintenance option for treating wastewater. This is particularly the case for low-income communities.

The sanitation concept ‘Blue Diversion’ ([www.bluediversiontoilet.com][1]) was developed as a possible answer to the sanitation crisis in urban slums. It is based on two main elements: (1) diversion of urine, feces, and water at the source as the basis for efficient resource recovery, and (2) linking different scales (family toilets and semi-centralized resource recovery). Our objective was to develop an attractive ‘grid-free’ (i.e. functioning without piped water, sewer, and electrical grid) dry urine-diverting toilet, which provides water (through recycling on-site) for flushing, personal hygiene (anal cleansing and menstrual hygiene), and hand washing. This service, including the entire sanitation value chain, should eventually be made available as a profitable business with total user fees of 5 ¢/p/d. The results presented in this paper are (1) a toilet design model, (2) the development of a new type of membrane bioreactor for treating flush and wash water, (3) main results of a geographic information system-based stochastic service model to link the family-scale toilet to a community-scale Resource Recovery Plant, and (4) a business model that yields maximum profit for the local community. We conclude that the approach is feasible, but challenging from a technical as well as an organizational point of view. [1]: http://www.bluediversiontoilet.com

Poor waste management is a key driver of ill-health in urban settlements of developing countries. The current study aimed at assessing environmental and human health risks related to urban waste management in Yamoussoukro, the political capital of Côte d’Ivoire. We undertook trans-disciplinary research within an Ecohealth approach, comprised of a participatory workshop with stakeholders and mapping of exposure patterns. A total of 492 randomly selected households participated in a cross-sectional survey. Waste deposit sites were characterised and 108 wastewater samples were subjected to laboratory examinations. The physico-chemical parameters of the surface water (temperature, pH, conductivity, potential oxidise reduction, BOD5, COD, dissolved oxygen, nitrates, ammonia and total Kendal nitrogen) did not comply with World Health Organization standards of surface water quality. Questionnaire results showed that malaria was the most commonly reported disease. Diarrhoea and malaria were associated with poor sanitation. Households having dry latrines had a higher risk of diarrhoea (odds ratio (OR) = 1.8, 95% confidence interval (CI) 1.2–2.7) compared to latrines with septic tanks and also a higher risk for malaria (OR = 1.9, 95% (CI) 1.1–3.3). Our research showed that combining health and environmental assessments enables a deeper understanding of environmental threats and disease burdens linked to poor waste management. Further study should investigate the sanitation strategy aspects that could reduce the environmental and health risks in the study area.

This paper discusses the notions of ‘improved’ and ‘unimproved’ sanitation in the context of developing countries in urbanizing Africa and considers the role that shared facilities can play in this equation. It analyses current definitions and classifications used by the United Nations Joint Monitoring Programme (JMP) to monitor access to shared sanitation and summarizes the recent discourse on JMP's limitations. Empirical evidence from two case studies in sub-Saharan Africa (SSA) - Kampala, Uganda and Ashaiman, Ghana - is presented, showing the widespread use and limitations of the shared toilet facilities in these two cities. The empirical evidence shows that quite different types of shared sanitation facilities emerge in different cities, each influenced by the urbanization patterns, local politics and sociocultural considerations. Improving the quality of shared facilities involves the consideration of the applicability of the different types of facilities within the category ‘shared sanitation’, toge...

Access to safe drinking water and improved sanitation is a key public health measure to prevent outbreak of diseases such as diarrhoea. We conducted a cross-sectional survey in 50 randomly selected slums of Kampala to assess the sources of water and sanitation facilities used in urban informal settlements. A total of 1,500 household respondents were interviewed. More than half (63.6%) of the respondents were using piped water for their domestic needs. The majority of the respondents (68.3%) had shared sanitation facilities and only 20% of the respondents had private ones. The factors influencing access to sanitation facilities included; household ownership, number of families sharing a toilet stand, cost of the sanitation facilities, stability of the income of household members and cleanliness of the facilities used. This paper thus provides knowledge insights on which more sustainable options for water and sanitation technologies in urban poor settlements can be based.

This paper aims to contribute to the growing body of literature on evaluation of community participation in the water and sanitation sector. The first part discusses the conceptual underpinnings of participatory approaches. The paper then analyses stakeholder perceptions about the Household-centred Environmental Sanitation (HCES) approach, a participatory planning approach recently validated in two countries: Lao People's Democratic Republic (PDR) and Nepal. Post intervention surveys were conducted with experts and key informants in both countries to assess satisfaction regarding degree of participation, effectiveness of planning outcomes and process efficiency of the participatory planning process. It specifically looks at the variability in people's perceptions about the costs and benefits of community participation. Empirical findings show that experts and participants show high satisfaction rates regarding involvement in decision making. The earlier and stronger residents were involved in the process, the higher the satisfaction rate. In a second part, the main findings of expert interviews are contrasted with the perceptions of the community at large which participated in the participatory planning process. A better understanding of community participation in urban settings is needed regarding skills, motivation, time, and defining the right levels of participation.

The multicomponent venom of the spider Cupiennius salei was separated by three different chromatographic strategies to facilitate subsequent analysis of peptidic venom components by tandem mass spectrometry (MALDI-TOF-MS and ESI-MS), Edman degradation and amino acid analysis: (a) desalting of the crude venom by RP-HPLC only, (b) chromatographic separation of the crude venom into 42 fractions by RP-HPLC, and (c) multidimensional purification of the crude venom by size exclusion and cation exchange chromatography and RP-HPLC. A total of 286 components were identified in the venom of C. salei by mass spectrometry and the sequence of 49 new peptides was determined de novo by Edman degradation and tandem mass spectrometry; 30 were C-terminally amidated. The novel peptides were assigned to two main groups: (a) short cationic peptides and (b) Cys-containing peptides with the inhibitor cystine knot motif. Bioinformatics revealed a limited number of substantial similarities, namely with the peptides CpTx1 from the spider Cheiracantium punctorium and U3-ctenitoxin-Asp1a from the South American fishing spider (Ancylometes sp.) and with sequences from a Lycosa singoriensis venom gland transcriptome analysis. The results clearly indicate that the quality of the data is strongly dependent on the chosen separation strategy. The combination of orthogonal analytical methods efficiently excludes alkali ion and matrix adducts, provides indispensable information for an unambiguous identification of isomasses, and results in the most comprehensive repertoire of peptides identified in the venom of C. salei so far.

The Household-Centered Environmental Sanitation (HCES) planning approach was tested for the first time in Nepal in a peri-urban setting during 2009/2011, in order to validate the novel planning approach, identify challenges and improve the process. The participatory multi-stakeholder process involved household mapping and surveys, user needs identification and prioritization and a stakeholder assessment. Following an expert's assessment of potential sanitation options, community sensitization campaigns through exposure visits, a sanitation bazaar and focused community interactions were conducted. Among the three sanitation alternatives, users showed strong preference to set up a simplified sewerage system with a decentralized wastewater treatment. The paper critically discusses the key challenges faced when developing environmental sanitation plans. Setting the right balance between empowering people to take informed decisions and keeping the participation process intact until the final stage was a major challenge. Although participatory planning is time consuming, it is worth investing as it builds local ownership and assists in informed decision-making processes for selecting affordable sanitation options that best meet user's needs.

Climate change, diminishing natural resources and rural-urban demographic trends will have profound impacts on future urban infrastructure delivery in both developed and developing countries. These challenges will however, leverage new opportunities for circular urban economies in which productive sanitation will play an important role in both the North and South. In the developed world, the challenge is to initiate a transition from disposal oriented, water-based infrastructure regimes towards more sustainable, re-use oriented, and productive sanitation. Decentralized approaches to “productive sanitation“ (including the production of biogas, fertilizer or water for irrigation) with a source-separation focus (segregation of flow streams with different properties) will allow for considerable cost and resource savings and enable life-cycle economies. In the developing world, the sanitation challenge is about leapfrogging over-engineered sanitation services and technologies, especially for urban areas which are currently without sanitation services, to overcome the huge service backlog. This paper gives an initial overview of the current state of urban sanitation with a North- South perspective, followed by a discussion of the new role of sustainable sanitation systems for cities of the future. Planning innovations for urban sanitation, initial lessons learned and current challenges faced are addressed. Context specific challenges and opportunities are illustrated in a variety of urban settings, from non-tenured, low-income settlements to middle- and high-income inner-city areas.

Urban sanitation presents one of the most significant service delivery challenges related to poverty alleviation and sustainable development in the developing world. The past decade has witnessed innovations in service delivery approaches for un-served rural and urban settlements with a clear policy shift to community-based approaches which attempt to overcome the supply-led, over-engineered sanitation solutions of the past decades. This paper presents two examples of new developments: the urban-focused Household-centred Environmental Sanitation (HCES) and the rural-focused Community-led Total Sanitation (CLTS) approaches. The internationally renowned CLTS approach has achieved considerable success since its introduction, by harnessing community and small private sector capacity to solve sanitation problems locally. Experience with validation of the HCES approach in a variety of urban sites in Africa, Asia and Latin America is presented in the second part of the paper- highlighting some of the lessons learned. The paper closes by arguing that a combination of HCES and CLTS, two field-tested methodologies, has the potential to improve the sustainability of sanitation service interventions.

Too often, standardized groupings of sanitation and treatment technologies are imposed in situations where they may not be appropriate. The Household-Centred Environmental Sanitation (HCES) approach instead emphasizes that the needs and means of households should be put first, and a collaborative process involving all stakeholders should steer the planning process.

Environmental Science & Technology, 48 (17), ISSN:0013-936X, ISSN:1520-5851

The multicomponent venom of the spider Cupiennius salei was separated by three different chromatographic strategies to facilitate subsequent analysis of peptidic venom components by tandem mass spectrometry (MALDI-TOF-MS and ESI-MS), Edman degradation and amino acid analysis: (a) desalting of the crude venom by RP-HPLC only, (b) chromatographic separation of the crude venom into 42 fractions by RP-HPLC, and (c) multidimensional purification of the crude venom by size exclusion and cation exchange chromatography and RP-HPLC. A total of 286 components were identified in the venom of C. salei by mass spectrometry and the sequence of 49 new peptides was determined de novo by Edman degradation and tandem mass spectrometry; 30 were C-terminally amidated. The novel peptides were assigned to two main groups: (a) short cationic peptides and (b) Cys-containing peptides with the inhibitor cystine knot motif. Bioinformatics revealed a limited number of substantial similarities, namely with the peptides CpTx1 from the spider Cheiracantium punctorium and U3-ctenitoxin-Asp1a from the South American fishing spider (Ancylometes sp.) and with sequences from a Lycosa singoriensis venom gland transcriptome analysis. The results clearly indicate that the quality of the data is strongly dependent on the chosen separation strategy. The combination of orthogonal analytical methods efficiently excludes alkali ion and matrix adducts, provides indispensable information for an unambiguous identification of isomasses, and results in the most comprehensive repertoire of peptides identified in the venom of C. salei so far.

Genetically modified (GM) legumes expressing the α-amylase inhibitor 1 (αAI-1) from Phaseolus vulgaris L. or cysteine protease inhibitors are resistant to several bruchid pests (Coleoptera: Chrysomelidae). In addition, the combination of plant resistance factors together with hymenopteran parasitoids can substantially increase the bruchid control provided by the resistance alone. If the strategy of combining a bruchid-resistant GM legume and biological control is to be effective, the insecticidal trait must not adversely affect bruchid antagonists. The environmental risk assessment of such GM legumes includes the characterization of the targeted enzymes in the beneficial species and the assessment of the in vitro susceptibility to the resistance factor. The digestive physiology of bruchid parasitoids remain relatively unknown, and their susceptibility to αAI-1 has never been investigated. We have detected α-amylase and serine protease activities in all five bruchid parasitoid species tested. Thus, the deployment of GM legumes expressing cysteine protease inhibitors to control bruchids should be compatible with the use of parasitoids. In vitro inhibition studies showed that sensitivity of α-amylase activity to αAI-1 in the parasitoids was comparable to that in the target species. Direct feeding assays revealed that harmful effects of α-amylase inhibitors on bruchid parasitoids cannot be discounted and need further evaluation.

Genetically modified (GM) cowpea seeds expressing αAI-1, an α-amylase inhibitor from the common bean, have been shown to be immune against several bruchid species. Effective control of such pests by growing GM cowpea could promote the spread of bruchid species that are αAI-1 tolerant. Consequently, the sustainability of bruchid pest control could be increased by combining GM seeds and hymenopteran parasitoids. However, there are concerns that αAI-1 could interfere with the biological control provided by parasitoids. Here, we assessed the impact of GM cowpea seeds expressing αAI-1 on the αAI-1-tolerant bruchid Zabrotes subfasciatus and its parasitoid Dinarmus basalis. αAI-1 in cowpea seeds did not increase resistance to Z. subfasciatus or affect the mortality rate of Z. subfasciatus larvae. Parasitism of Z. subfasciatus by D. basalis and fitness of D. basalis offspring were not affected by the presence of αAI-1. Thus, αAI-1-expressing cowpeas and parasitoids should be compatible for the control of bruchid pests.