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Formalin is a dangerous food preservative which has been banned to be used. Formalin is often used because it is relatively cheap and simple to use. This study aimed to analyze the effect of formalin to damage liver histology of male mice. Mice used were male mice Balb/C strain aged 4 weeks and weighted 20 to 22 grams. The extract of formalin-added-tofu, which were the concentration of: 0.1%, 1.68%, and 3.35%, 5.1%, 6.7%, were given orally per day for 12 weeks. At the end of 12th week, the mice were dissected and the liver were taken which then made preparations. The liver histological preparations were observed under a dino lite microscope in five different visual field, with a magnification of 800 times. For each of field of views, 20 cells were randomly counted. The average of weighted score of histopathological liver of five field of views then were randomly calculated for each mice. The scoring model used was Histopathology Manja Roenigk. The total of damage percentage occurred was recorded and calculated. The results showed that oral exposure to formaldehyde increases the amount of damage significantly in male mice strain Balb/C. The research results were presented in the form of booklets as a means of communication to the public media to broaden the danger of formalin-added-tofu. The booklet was made in order to perform the role of education as an agent of society renewal insight and knowledge. In addition, it also creating a continuous learning.Keywords: Booklet, Formalin, Hepar Histology, Male Mice, Tofu

PURPOSE: To investigate the hepatotoxicity and nephrotoxicity of 3-Bromopyruvate (3BP) in mice. METHODS: Fifteen nude mice were grafted subcutaneously in the left flank with MDA-MB-231 cells, then all mice were divided into control group (PBS), 3BP group (8 mg/kg), positive group (DNR: 0.8 mg/kg) when tumor volume reached approximately 100 mm 3 . 28 days later, tumors, livers and kidneys were stored in 4 % formalin solution and stained with hematoxylin and eosin staining. The Kunming mice experiment included control group (PBS), 3BP group (4mg/kg; 8mg/kg; 16mg/kg), positive group (DNR: 0.8 mg/kg). 24 hours later, the blood were used for the determination of hepatic damage serum biomarkers. Livers were stored in 4 % formalin solution for the later detection. RESULTS: 3BP at the dose of 8mg/kg had a good effect on inhibiting tumor growth in nude mice and did not damage liver and kidney tissues. Kunming mice experiment showed 3BP at the dose of 16mg/kg did damage to liver tissues. CONCLUSION: 3-Bromopyruvate at the dose of suppressing tumor growth did not exhibit hepatotoxicity and nephrotoxicity in nude mice, and the effect on liver was confirmed in Kunming mice. Key words: Bromopyruvate; Hepatotoxicity; Nephrotoxicity; Mice

The formation oxytetracycline (OTC) degradation products in chicken and pork under two different methods of cooking were studied. Samples of chicken and pig muscles previously dosed with OTC residues were subjected to boiling or microwave treatment, and the residues were extracted in a mixture of citrate buffer-MeOH (75:25 v/v), and then analyzed by high performance liquid chromatography with photodiode array detection using a XBridgeTM C18 reverse-phase chromatographic column. Thermal treatment resulted in the degradation of OTC and the concentrations of the degradation products α-apo-oxytetracycline (α-apo-OTC) and β-apo-oxytetracycline (β-apo-OTC) in muscle samples amounted to 0.7 to 1.2 % of the initial OTC content. The toxic effects of the degradation products of oxytetracycline, α-apo-OTC and β-apo-OTC were studied in rats. Male rats received oral doses of 10 mg/kg body weight/day of either α-apo-OTC, β-apo-OTC, 90 days. The results of this study suggest that the toxic effects of β-apo-OTC treatment could damage liver and kidney tissues of rats, as well as lead to the degeneration and necrosis in the hepatocytes.