62 results on '"Danielle Monniaux"'
Search Results
2. Efficacy of a single blood anti-Müllerian hormone (AMH) concentration measurement for the selection of Japanese Black heifer embryo donors in herd breeding programs
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Yasuo FUSHIMI, Hiroaki OKAWA, Danielle MONNIAUX, and Mitsuhiro TAKAGI
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anti-müllerian hormone ,cattle intensive breeding ,japanese black cattle ,ovum pick-up ,Reproduction ,QH471-489 ,Internal medicine ,RC31-1245 - Abstract
We evaluated the relationship between plasma anti-Müllerian hormone (AMH) concentrations in Japanese Black (JB) heifers at 7−10 months of age and the number of embryos recovered after superovulation treatment in selected ovum pick-up donors, concomitantly with changes in their AMH concentrations before and after parturition. Plasma AMH concentrations in heifers were positively correlated with the total number of follicles (r = 0.647, P < 0.01) and embryos (r = 0.681, P < 0.01) recovered from the animals postpartum, when selected as donor cows, but did not correlate with the total number of transferable embryos. No difference was observed between the plasma AMH concentration at the heifer period and the postpartum period. Additionally, serum AMH concentrations of heifers weakly correlated with the number of follicles and embryos recovered by virgin flush after superovulation treatment at 13−15 months of age. Therefore, a single blood AMH concentration measurement may accelerate intensive JB cattle breeding.
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- 2020
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3. Efficacy of a single measurement of plasma anti-Müllerian hormone concentration for ovum pick-up donor selection of Japanese Black heifers in herd breeding programs
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Yasuo FUSHIMI, Danielle MONNIAUX, and Mitsuhiro TAKAGI
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anti-müllerian hormone ,cattle intensive breeding ,japanese black cattle ,ovum pick-up ,Reproduction ,QH471-489 ,Internal medicine ,RC31-1245 - Abstract
In this study, we evaluated the efficiency of a single measurement of plasma anti-Müllerian hormone (AMH) concentration in heifers in determining the number of oocytes recoverable by ovum pick-up (OPU), and compared AMH concentrations among sister heifers from the same parents. For this, blood samples from 50 embryo-transfer-derived female Japanese Black (JB) heifers (mean: 8.7 age in months) were collected and plasma AMH concentration was measured. At 13–15 months of age, both the number of follicles (2–9 mm) and the number of collected oocytes after OPU were counted and compared. Results indicated that the heifers with the highest AMH concentration had the highest number of follicles in their ovaries and gave the highest number of collected oocytes with OPU, thereby indicating that a single measurement of plasma AMH concentration is informative for the selection of OPU-donor heifers in herd breeding programs. The practice of performing a single AMH measurement may accelerate the intensive breeding of JB herds.
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- 2019
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4. The Crazy Ovary
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Philippe Monget, Ken McNatty, and Danielle Monniaux
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ovary ,folliculogenesis ,reactivation ,primordial follicles ,ovulation rate ,unexpected ,Genetics ,QH426-470 - Abstract
From fetal life until senescence, the ovary is an extremely active tissue undergoing continuous structural and functional changes. These ever-changing events are best summarized by a quotation attributed to Plato when describing motion in space and time—‘nothing ever is but is always becoming…’. With respect to the ovary, these changes include, at the beginning, the processes of follicular formation and thereafter those of follicular growth and atresia, steroidogenesis, oocyte maturation, and decisions relating to the number of mature oocytes that are ovulated for fertilization and the role of the corpus luteum. The aims of this review are to offer some examples of these complex and hitherto unknown processes. The ones herein have been elucidated from studies undertaken in vitro or from normal in vivo events, natural genetic mutations or after experimental inactivation of gene function. Specifically, this review offers insights concerning the initiation of follicular growth, pathologies relating to poly-ovular follicles, the consequences of premature loss of germ cells or oocytes loss, the roles of AMH (anti-Müllerian hormone) and BMP (bone morphogenetic protein) genes in regulating follicular growth and ovulation rate together with species differences in maintaining luteal function during pregnancy. Collectively, the evidence suggests that the oocyte is a key organizer of normal ovarian function. It has been shown to influence the phenotype of the adjacent somatic cells, the growth and maturation of the follicle, and to determine the ovulation rate. When germ cells or oocytes are lost prematurely, the ovary becomes disorganized and a wide range of pathologies may arise.
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- 2021
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5. Association between Anti-Müllerian Hormone Concentration and Inflammation Markers in Serum during the Peripartum Period in Dairy Cows
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Hiroaki Okawa, Danielle Monniaux, Chihiro Mizokami, Atsushi Fujikura, Toshihiro Takano, Satoko Sato, Urara Shinya, Chiho Kawashima, Osamu Yamato, Yasuo Fushimi, Peter L. A. M. Vos, Masayasu Taniguchi, and Mitsuhiro Takagi
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anti-Müllerian hormone ,dairy cattle ,endometritis ,inflammation ,reproductive efficacy ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
The relationships between changes in anti-Müllerian hormone (AMH) concentration and various traits, including milk somatic cell counts (SCC), were evaluated. Blood samples were collected from 43 Holstein cows 14 days before (D-14) and 10 (D10) and 28 days after (D28) parturition, and vaginal discharge score (VDS) and polymorphonuclear leukocyte (PMNL) percentages were assessed in endometrial samples at D28. Cows were separated into four quartiles (Q1–Q4) based on changes in AMH concentration during the peripartum period (AMH ratio: D28/D-14). Correlations between AMH ratio and each parameter were evaluated and classified into high-AMH (Q4, 1.83 ± 0.12, n = 11) and low-AMH (Q1, 0.83 ± 0.05, n = 11) groups. The AMH ratio was positively correlated with magnesium and non-esterified fatty acids levels, and the albumin/globulin ratio at D10 and D28, but negatively correlated with serum amyloid A (SAA) at D10. SAA and γ-globulin levels were significantly higher in the low-AMH group at D28. There was no significant difference in VDS, PMNL percentage, and milk SCC between the two groups. The decreasing AMH ratio from the prepartum to the postpartum period corresponds to high inflammation biomarker levels. Whether it subsequently affects the reproductive prognosis of postpartum cows needs further investigations.
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- 2021
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6. A Comparative Analysis of Oocyte Development in Mammals
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Rozenn Dalbies-Tran, Véronique Cadoret, Alice Desmarchais, Sébastien Elis, Virginie Maillard, Philippe Monget, Danielle Monniaux, Karine Reynaud, Marie Saint-Dizier, and Svetlana Uzbekova
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oocyte ,mammals ,evolution ,gene expression ,posttranscriptional control ,Gdf9 ,Cytology ,QH573-671 - Abstract
Sexual reproduction requires the fertilization of a female gamete after it has undergone optimal development. Various aspects of oocyte development and many molecular actors in this process are shared among mammals, but phylogeny and experimental data reveal species specificities. In this chapter, we will present these common and distinctive features with a focus on three points: the shaping of the oocyte transcriptome from evolutionarily conserved and rapidly evolving genes, the control of folliculogenesis and ovulation rate by oocyte-secreted Growth and Differentiation Factor 9 and Bone Morphogenetic Protein 15, and the importance of lipid metabolism.
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- 2020
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7. The highly prolific phenotype of Lacaune sheep is associated with an ectopic expression of the B4GALNT2 gene within the ovary.
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Laurence Drouilhet, Camille Mansanet, Julien Sarry, Kamila Tabet, Philippe Bardou, Florent Woloszyn, Jérome Lluch, Grégoire Harichaux, Catherine Viguié, Danielle Monniaux, Loys Bodin, Philippe Mulsant, and Stéphane Fabre
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Genetics ,QH426-470 - Abstract
Prolific sheep have proven to be a valuable model to identify genes and mutations implicated in female fertility. In the Lacaune sheep breed, large variation in litter size is genetically determined by the segregation of a fecundity major gene influencing ovulation rate, named FecL and its prolific allele FecL(L) . Our previous work localized FecL on sheep chromosome 11 within a locus of 1.1 Mb encompassing 20 genes. With the aim to identify the FecL gene, we developed a high throughput sequencing strategy of long-range PCR fragments spanning the locus of FecL(L) carrier and non-carrier ewes. Resulting informative markers defined a new 194.6 kb minimal interval. The reduced FecL locus contained only two genes, insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) and beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), and we identified two SNP in complete linkage disequilibrium with FecL(L) . B4GALNT2 appeared as the best positional and expressional candidate for FecL, since it showed an ectopic expression in the ovarian follicles of FecL(L) /FecL(L) ewes at mRNA and protein levels. In FecL(L) carrier ewes only, B4GALNT2 transferase activity was localized in granulosa cells and specifically glycosylated proteins were detected in granulosa cell extracts and follicular fluids. The identification of these glycoproteins by mass spectrometry revealed at least 10 proteins, including inhibin alpha and betaA subunits, as potential targets of B4GALNT2 activity. Specific ovarian protein glycosylation by B4GALNT2 is proposed as a new mechanism of ovulation rate regulation in sheep, and could contribute to open new fields of investigation to understand female infertility pathogenesis.
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- 2013
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8. Correction: Postnatal Leptin Promotes Organ Maturation and Development in IUGR Piglets.
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Linda Attig, Daphné Brisard, Thibaut Larcher, Michal Mickiewicz, Paul Guilloteau, Samir Boukthir, Claude-Narcisse Niamba, Arieh Gertler, Jean Djiane, Danielle Monniaux, and Latifa Abdennebi-Najar
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Medicine ,Science - Published
- 2013
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9. Postnatal leptin promotes organ maturation and development in IUGR piglets.
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Linda Attig, Daphné Brisard, Thibaut Larcher, Michal Mickiewicz, Paul Guilloteau, Samir Boukthir, Claude-Narcisse Niamba, Arieh Gertler, Jean Djiane, Danielle Monniaux, and Latifa Abdennebi-Najar
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Medicine ,Science - Abstract
Babies with intra-uterine growth restriction (IUGR) are at increased risk for experiencing negative neonatal outcomes due to their general developmental delay. The present study aimed to investigate the effects of a short postnatal leptin supply on the growth, structure, and functionality of several organs at weaning. IUGR piglets were injected from day 0 to day 5 with either 0.5 mg/kg/d leptin (IUGRLep) or saline (IUGRSal) and euthanized at day 21. Their organs were collected, weighed, and sampled for histological, biochemical, and immunohistochemical analyses. Leptin induced an increase in body weight and the relative weights of the liver, spleen, pancreas, kidneys, and small intestine without any changes in triglycerides, glucose and cholesterol levels. Notable structural and functional changes occurred in the ovaries, pancreas, and secondary lymphoid organs. The ovaries of IUGRLep piglets contained less oogonia but more oocytes enclosed in primordial and growing follicles than the ovaries of IUGRSal piglets, and FOXO3A staining grade was higher in the germ cells of IUGRLep piglets. Within the exocrine parenchyma of the pancreas, IUGRLep piglets presented a high rate of apoptotic cells associated with a higher trypsin activity. In the spleen and the Peyer's patches, B lymphocyte follicles were much larger in IUGRLep piglets than in IUGRSal piglets. Moreover, IUGRLep piglets showed numerous CD79(+) cells in well-differentiated follicle structures, suggesting a more mature immune system. This study highlights a new role for leptin in general developmental processes and may provide new insight into IUGR pathology.
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- 2013
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10. Anti-Müllerian hormone production in the ovary: a comparative study in bovine and porcine granulosa cells†
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Peggy Jarrier, Danielle Monniaux, Eric Venturi, Anthony Estienne, Christophe Staub, Yves Le Vern, Philippe Monget, Nathalie di Clemente, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Infectiologie et Santé Publique (UMR ISP), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), French Fellowship from the Région centre and INRAE, ANR-12-BSV1-0034,AMHAROC,L'hormone anti-Müllérienne ovarienne : régulation, activité et implication dans le syndrome des ovaires polykystiques(2012), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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Anti-Mullerian Hormone ,pig ,Smad6 Protein ,Swine ,[SDV]Life Sciences [q-bio] ,Response element ,Ovary ,Biology ,Bone morphogenetic protein ,03 medical and health sciences ,granulosa ,0302 clinical medicine ,Species Specificity ,AMH ,Protein biosynthesis ,medicine ,Animals ,Promoter Regions, Genetic ,030304 developmental biology ,0303 health sciences ,Messenger RNA ,Granulosa Cells ,promoter ,030219 obstetrics & reproductive medicine ,Gene Expression Regulation, Developmental ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Anti-Müllerian hormone ,Cell Biology ,General Medicine ,Transfection ,Immunohistochemistry ,Molecular biology ,medicine.anatomical_structure ,Reproductive Medicine ,cattle ,Bone Morphogenetic Proteins ,biology.protein ,Female ,Signal Transduction ,Hormone - Abstract
In this study, we aimed to determine the origin of the difference, in terms of anti-Müllerian hormone production, existing between the bovine and porcine ovaries. We first confirmed by quantitative real-time-Polymerase-Chain Reaction, ELISA assay and immunohistochemistry that anti-Müllerian hormone mRNA and protein production are very low in porcine ovarian growing follicles compared to bovine ones. We then have transfected porcine and bovine granulosa cells with vectors containing the luciferase gene driven by the porcine or the bovine anti-Müllerian hormone promoter. These transfection experiments showed that the porcine anti-Müllerian hormone promoter is less active and less responsive to bone morphogenetic protein stimulations than the bovine promoter in both porcine and bovine cells. Moreover, bovine but not porcine granulosa cells were responsive to bone morphogenetic protein stimulation after transfection of a plasmidic construction including a strong response element to the bone morphogenetic proteins (12 repetitions of the GCCG sequence) upstream of the luciferase reporter gene. We also showed that SMAD6, an inhibitor of the SMAD1-5-8 pathway, is strongly expressed in porcine compared to the bovine granulosa cells. Overall, these results suggest that the low expression of anti-Müllerian hormone in porcine growing follicles is due to both a lack of activity/sensitivity of the porcine anti-Müllerian hormone promoter, and to the lack of responsiveness of porcine granulosa cells to bone morphogenetic protein signaling, potentially due to an overexpression of SMAD6 compared to bovine granulosa cells. We propose that the low levels of anti-Müllerian hormone in the pig would explain the poly-ovulatory phenotype in this species.
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- 2020
11. Investigating the role of BCAR4 in ovarian physiology and female fertility by genome editing in rabbit
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Rozenn Dalbies-Tran, Maud Peyny, Peggy Jarrier-Gaillard, Sébastien Lavillatte, Laurent Boulanger, Nathalie Daniel, Pascal Papillier, Véronique Duranthon, Geneviève Jolivet, Véronique Cadoret, Danielle Monniaux, Nathalie Peynot, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Plateforme d'Infectiologie Expérimentale (PFIE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CHU Bretonneau, Médecine et Biologie de la Reproduction-CECOS, INRAE, Agence Nationale de la Biomédecine, Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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0301 basic medicine ,rabbits ,media_common.quotation_subject ,[SDV]Life Sciences [q-bio] ,Embryonic Development ,Gene Expression ,Reproductive biology ,lcsh:Medicine ,Ovary ,Biology ,Oogenesis ,Article ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Ovarian Follicle ,Follicular phase ,medicine ,Animals ,Blastocyst ,Ovarian follicle ,lcsh:Science ,Ovulation ,genome ,media_common ,Gene Editing ,fertility ,Multidisciplinary ,lcsh:R ,Functional genomics ,Oocyte ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,ovulation ,Female ,RNA, Long Noncoding ,lcsh:Q ,Folliculogenesis ,Anti-estrogen - Abstract
Breast Cancer Anti-estrogen Resistance 4 (BCAR4) was previously characterised in bovine species as a gene preferentially expressed in oocytes, whose inhibition is detrimental to in vitro embryo development. But its role in oogenesis, folliculogenesis and globally fertility in vivo remains unknown. Because the gene is not conserved in mice, rabbits were chosen for investigation of BCAR4 expression and function in vivo. BCAR4 displayed preferential expression in the ovary compared to somatic organs, and within the ovarian follicle in the oocyte compared to somatic cells. The transcript was detected in follicles as early as the preantral stage. Abundance decreased throughout embryo development until the blastocyst stage. A lineage of genome-edited rabbits was produced; BCAR4 expression was abolished in follicles from homozygous animals. Females of wild-type, heterozygous and homozygous genotypes were examined for ovarian physiology and reproductive parameters. Follicle growth and the number of ovulations in response to hormonal stimulation were not significantly different between genotypes. Following insemination, homozygous females displayed a significantly lower delivery rate than their heterozygous counterparts (22 ± 7% vs 71 ± 11% (mean ± SEM)), while prolificacy was 1.8 ± 0.7 vs 6.0 ± 1.4 kittens per insemination. In conclusion, BCAR4 is not essential for follicular growth and ovulation but it contributes to optimal fertility in rabbits.
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- 2020
12. In vitro survival of follicles in prepubertal ewe ovarian cortex cryopreserved by slow freezing or non-equilibrium vitrification
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Danielle Monniaux, Nicolas Duffard, Yann Locatelli, L Lardic, Pascal Mermillod, Michael J. Bertoldo, P Piver, Laure Calais, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Direction générale déléguée aux musées et aux jardins botaniques et zoologiques (DGD.MJZ), Muséum national d'Histoire naturelle (MNHN), Institut National de la Recherche Agronomique (INRA), Service de Gynécologie-Obstétrique [CHU Limoges], CHU Limoges, School of Women's and Children's Health [Sydney, Australia], Sydney Children's hospital-University of New South Wales [Sydney] (UNSW), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and University of New South Wales [Sydney] (UNSW)-Sydney Children's hospital
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0301 basic medicine ,Ovarian Cortex ,Population ,Biology ,Cryopreservation ,[SHS]Humanities and Social Sciences ,Tissue Culture Techniques ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Ovarian Follicle ,Fresh Tissue ,Proliferating Cell Nuclear Antigen ,Genetics ,Animals ,Vitrification ,Fertility preservation ,education ,Progesterone ,Genetics (clinical) ,education.field_of_study ,Sheep ,030219 obstetrics & reproductive medicine ,Estradiol ,Ovary ,Puberty ,Fertility Preservation ,Obstetrics and Gynecology ,General Medicine ,030104 developmental biology ,Reproductive Medicine ,Cryopreserved Tissue ,Female ,Folliculogenesis ,Developmental Biology - Abstract
PURPOSE: Vitrification is a well-accepted fertility preservation procedure for cryopreservation of oocytes and embryos but little is known regarding ovarian tissue, for which slow freezing is the current convention. The aim of the present study was to assess the efficiency of non-equilibrium vitrification compared to conventional slow freezing for ovarian cortex cryopreservation. METHODS: Using prepubertal sheep ovaries, the capacity of the tissue to sustain folliculogenesis following cryopreservation and in vitro culture was evaluated. Ovarian cortex fragments were cultured in wells for 9 days, immediately or after cryopreservation by conventional slow freezing or non-equilibrium vitrification in straws. During culture, follicular populations within cortex were evaluated by histology and immunohistochemistry for PCNA and TUNEL. Steroidogenic activity of the tissue was monitored by assay for progesterone and estradiol in spent media. RESULTS: No significant differences in follicle morphology, PCNA, or TUNEL labeling were observed between cryopreservation methods at the initiation of culture. Similar decreases in the proportion of primordial follicle population, and increases in the proportion of growing follicles, were observed following culture of fresh or cryopreserved ovarian tissue regardless of cryopreservation method. At the end of culture, PCNA and TUNEL-positive follicles were not statistically altered by slow freezing or vitrification in comparison to fresh cultured fragments. CONCLUSIONS: Overall, for both cryopreservation methods, the cryopreserved tissue showed equal capacity to fresh tissue for supporting basal folliculogenesis in vitro. Taken together, these data confirm that both non-equilibrium vitrification and slow-freezing methods are both efficient for the cryopreservation of sheep ovarian cortex fragments.
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- 2019
13. Prenatal programming by testosterone of follicular theca cell functions in ovary
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Stéphane Fabre, Rozenn Dalbies-Tran, Hans Adriaensen, Juliette Cognie, Nathalie di Clemente, Anthony Estienne, Danielle Monniaux, Virginie Maillard, Carine Genet, Christelle Hennequet-Antier, Peggy Jarrier, Corinne Laclie, Florence Plisson-Petit, Pascal Papillier, Anne-Lyse Lainé, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut National de la Santé et de la Recherche Médicale (INSERM), Region Centre and INRA, France - French fellowshipFrench National Research Agency (ANR) - AMHAROC/ANR-12-BSV1-0034-02, Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur], and Institut National de la Recherche Agronomique (INRA)-Université de Tours
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collagen ,sheep ,endocrine system ,medicine.medical_specialty ,Testosterone Excess ,endocrine system diseases ,Ovarian Cortex ,[SDV]Life Sciences [q-bio] ,fibrillin ,Biology ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Ovarian Follicle ,Pregnancy ,Internal medicine ,Follicular phase ,medicine ,Animals ,Humans ,rna sequencing ,Gene Regulatory Networks ,Testosterone ,Molecular Biology ,Cells, Cultured ,mri ,030304 developmental biology ,Pharmacology ,0303 health sciences ,030219 obstetrics & reproductive medicine ,pcos ,Theca Cell ,Cell Biology ,Antral follicle ,Follicular fluid ,smoc ,Endocrinology ,Gene Expression Regulation ,Theca ,Prenatal Exposure Delayed Effects ,Theca Cells ,amh ,Molecular Medicine ,Female ,Polycystic Ovary Syndrome ,Hormone - Abstract
International audience; In mammalian ovaries, the theca layers of growing follicles are critical for maintaining their structural integrity and supporting androgen synthesis. Through combining the postnatal monitoring of ovaries by abdominal magnetic resonance imaging, endocrine profiling, hormonal analysis of the follicular fluid of growing follicles, and transcriptomic analysis of follicular theca cells, we provide evidence that the exposure of ovine fetuses to testosterone excess activates postnatal follicular growth and strongly affects the functions of follicular theca in adulthood. Prenatal exposure to testosterone impaired androgen synthesis in the small antral follicles of adults and affected the expression in their theca cells of a wide array of genes encoding extracellular matrix components, their membrane receptors, and signaling pathways. Most expression changes were uncorrelated with the concentrations of gonadotropins, steroids, and anti-Müllerian hormone in the recent hormonal environment of theca cells, suggesting that these changes rather result from the long-term developmental effects of testosterone on theca cell precursors in fetal ovaries. Disruptions of the extracellular matrix structure and signaling in the follicular theca and ovarian cortex can explain the acceleration of follicle growth through altering the stiffness of ovarian tissue. We propose that these mechanisms participate in the etiology of the polycystic ovarian syndrome, a major reproductive pathology in woman.
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- 2019
14. Association between Anti-Müllerian Hormone Concentration and Inflammation Markers in Serum during the Peripartum Period in Dairy Cows
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Mitsuhiro Takagi, Masayasu Taniguchi, Chiho Kawashima, Danielle Monniaux, Satoko Sato, Urara Shinya, Osamu Yamato, Peter L.A.M. Vos, Toshihiro Takano, Chihiro Mizokami, Hiroaki Okawa, Yasuo Fushimi, Atsushi Fujikura, Yamaguchi University [Yamaguchi], Fukuoka Prefecture Dairy Cooperative Association, Guardian Co. Ltd., Partenaires INRAE, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Soo Agriculture Mutual Aid Association, Obihiro University of Agriculture and Veterinary Medicine, Kagoshima University, and Utrecht University [Utrecht]
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[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,endocrine system ,endometritis ,Globulin ,Veterinary medicine ,Article ,Andrology ,03 medical and health sciences ,SF600-1100 ,Medicine ,Serum amyloid A ,Peripartum Period ,Dairy cattle ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,reproductive efficacy ,General Veterinary ,biology ,business.industry ,urogenital system ,0402 animal and dairy science ,Albumin ,dairy cattle ,Anti-Müllerian hormone ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,04 agricultural and veterinary sciences ,medicine.disease ,040201 dairy & animal science ,female genital diseases and pregnancy complications ,anti-Müllerian hormone ,QL1-991 ,inflammation ,biology.protein ,Animal Science and Zoology ,Endometritis ,business ,Zoology ,Postpartum period ,hormones, hormone substitutes, and hormone antagonists - Abstract
Simple Summary Changes in anti-Müllerian hormone (AMH) levels, a fertility marker in dairy cows, during the peripartum period associated with reproductive recovery have not been fully evaluated. We investigated the relationship between changes in AMH concentration and inflammation markers in serum during the peripartum period in dairy cows. We found a relationship between changes in AMH concentration, especially reflected in the AMH ratio during the perinatal period, and the systemic inflammation status of dairy cows. Excessive inflammation during the early postpartum period may decrease AMH levels and subsequently affect the reproductive prognosis of postpartum cows. Elucidating the mechanism of perinatal AMH changes and the beneficial effects of AMH may improve reproductive efficacy in the dairy industry. Abstract The relationships between changes in anti-Müllerian hormone (AMH) concentration and various traits, including milk somatic cell counts (SCC), were evaluated. Blood samples were collected from 43 Holstein cows 14 days before (D-14) and 10 (D10) and 28 days after (D28) parturition, and vaginal discharge score (VDS) and polymorphonuclear leukocyte (PMNL) percentages were assessed in endometrial samples at D28. Cows were separated into four quartiles (Q1–Q4) based on changes in AMH concentration during the peripartum period (AMH ratio: D28/D-14). Correlations between AMH ratio and each parameter were evaluated and classified into high-AMH (Q4, 1.83 ± 0.12, n = 11) and low-AMH (Q1, 0.83 ± 0.05, n = 11) groups. The AMH ratio was positively correlated with magnesium and non-esterified fatty acids levels, and the albumin/globulin ratio at D10 and D28, but negatively correlated with serum amyloid A (SAA) at D10. SAA and γ-globulin levels were significantly higher in the low-AMH group at D28. There was no significant difference in VDS, PMNL percentage, and milk SCC between the two groups. The decreasing AMH ratio from the prepartum to the postpartum period corresponds to high inflammation biomarker levels. Whether it subsequently affects the reproductive prognosis of postpartum cows needs further investigations.
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- 2021
15. New Anti-Müllerian Hormone Target Genes Involved in Granulosa Cell Survival in Women With Polycystic Ovary Syndrome
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Stéphane Fabre, Alice Pierre, Emmanuelle Mathieu d'Argent, Corinne Vigouroux, Nathalie di Clemente, Carine Genet, Chrystèle Racine, Camille Bourgneuf, Charlotte Dupont, Christelle Hennequet-Antier, Danielle Monniaux, Julien Sarry, Florence Plisson-Petit, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CHU Tenon [AP-HP], Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Ligue nationale contre le cancerN/ref: RS16/75-41, ANR-12-BSV1-0034,AMHAROC,L'hormone anti-Müllérienne ovarienne : régulation, activité et implication dans le syndrome des ovaires polykystiques(2012), Fabre, Stéphane, BLANC - L'hormone anti-Müllérienne ovarienne : régulation, activité et implication dans le syndrome des ovaires polykystiques - - AMHAROC2012 - ANR-12-BSV1-0034 - BLANC - VALID, Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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Anti-Mullerian Hormone ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Apoptosis ,Anti-Müllerian hormone ,[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Biochemistry ,Mice ,0302 clinical medicine ,Endocrinology ,Cells, Cultured ,0303 health sciences ,[SDV.BDLR.RS] Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction ,030219 obstetrics & reproductive medicine ,biology ,Granulosa cells apoptosis ,Polycystic ovary ,female genital diseases and pregnancy complications ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Female ,hormones, hormone substitutes, and hormone antagonists ,Polycystic Ovary Syndrome ,Adult ,endocrine system ,medicine.medical_specialty ,Cell Survival ,Granulosa cell ,Mice, Transgenic ,[SDV.GEN.GA] Life Sciences [q-bio]/Genetics/Animal genetics ,Follicle atresia ,[SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction ,03 medical and health sciences ,Follicle ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Internal medicine ,medicine ,Animals ,Humans ,Gene ,030304 developmental biology ,Cell Proliferation ,Granulosa Cells ,urogenital system ,Biochemistry (medical) ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,medicine.disease ,Mice, Inbred C57BL ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,Gene Expression Regulation ,Atresia ,Case-Control Studies ,biology.protein ,Target genes ,Hormone - Abstract
Purpose A protective effect of anti-Müllerian hormone (AMH) on follicle atresia was recently demonstrated using long-term treatments, but this effect has never been supported by mechanistic studies. This work aimed to gain an insight into the mechanism of action of AMH on follicle atresia and on how this could account for the increased follicle pool observed in women with polycystic ovary syndrome (PCOS). Methods In vivo and in vitro experiments were performed to study the effects of AMH on follicle atresia and on the proliferation and apoptosis of granulosa cells (GCs). RNA-sequencing was carried out to identify new AMH target genes in GCs. The expression of some of these genes in GCs from control and PCOS women was compared using microfluidic real time quantitative RT-PCR. Results A short-term AMH treatment prevented follicle atresia in prepubertal mice. Consistent with this result, AMH inhibited apoptosis and promoted proliferation of different models of GCs. Moreover, integrative biology analyses of 965 AMH target genes identified in 1 of these GC models, confirmed that AMH had initiated a gene expression program favoring cell survival and proliferation. Finally, on 43 genes selected among the most up- and down-regulated AMH targets, 8 were up-regulated in GCs isolated from PCOS women, of which 5 are involved in cell survival. Main conclusions Our results provide for the first time cellular and molecular evidence that AMH protects follicles from atresia by controlling GC survival and suggest that AMH could participate in the increased follicle pool of PCOS patients.
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- 2020
16. Mathematical modeling approaches of cellular endocrinology within the hypothalamo-pituitary-gonadal axis
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Pascale Crépieux, Danielle Monniaux, Romain Yvinec, Frédérique Clément, Dynamiques de populations multi-échelles pour des systèmes physiologiques (MUSCA), Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE), and Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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0301 basic medicine ,Male ,medicine.medical_specialty ,Cell type ,Cell signaling ,endocrine system ,Hypothalamo-Hypophyseal System ,Somatic cell ,[SDV]Life Sciences [q-bio] ,Pituitary-Adrenal System ,030209 endocrinology & metabolism ,Hypothalamic–pituitary–gonadal axis ,Biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Hypothamo-pituitary-gonadal axis ,Endocrinology ,Pulsatile secretion ,Internal medicine ,medicine ,Endocrine system ,Animals ,Humans ,Gonads ,Molecular Biology ,Mathematical models ,Reproduction ,Cell population dynamics ,Models, Theoretical ,030104 developmental biology ,Female ,Endocrine Cells ,Function (biology) ,Hormone ,Signal Transduction - Abstract
International audience; The reproductive neuroendocrine axis, or hypothalamo-pituitary-gonadal (HPG) axis, is a paragon of complex biological system involving numerous cell types, spread over several anatomical levels communicating through entangled endocrine feedback loops. The HPG axis exhibits remarkable dynamic behaviors on multiple time and space scales, which are an inexhaustible source of studies for mathematical and computational biology. In this review, we will describe a variety of modeling approaches of the HPG axis from a cellular endocrinology viewpoint. We will in particular investigate the questions raised by some of the most striking features of the HPG axis: (i) the pulsatile secretion of hypothalamic and pituitary hormones, and its counterpart, the cell signaling induced by frequency-encoded hormonal signals, and (ii) the dual, gametogenic and glandular function of the gonads, which relies on the tight control of the somatic cell populations ensuring the proper maturation and timely release of the germ cells.
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- 2020
17. Efficacy of a single blood anti-Müllerian hormone (AMH) concentration measurement for the selection of Japanese Black heifer embryo donors in herd breeding programs
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Mitsuhiro Takagi, Danielle Monniaux, Hiroaki Okawa, Yasuo Fushimi, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Yamaguchi University [Yamaguchi], and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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Anti-Mullerian Hormone ,endocrine system ,animal structures ,Superovulation ,Fertilization in Vitro ,Breeding ,Anti-Müllerian hormone ,Japanese Black cattle ,Andrology ,Embryo Culture Techniques ,03 medical and health sciences ,0302 clinical medicine ,Animals ,Animal Husbandry ,Technology Report ,Selection (genetic algorithm) ,030304 developmental biology ,Ovum ,0303 health sciences ,030219 obstetrics & reproductive medicine ,biology ,Embryo ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Embryo Transfer ,Embryo, Mammalian ,Cattle intensive breeding ,biology.protein ,Herd ,Oocytes ,Animal Science and Zoology ,Cattle ,Female ,Ovum pick-up ,Postpartum period ,Hormone - Abstract
International audience; We evaluated the relationship between plasma anti-Müllerian hormone (AMH) concentrations in Japanese Black (JB) heifers at 7-10 months of age and the number of embryos recovered after superovulation treatment in selected ovum pick-up donors, concomitantly with changes in their AMH concentrations before and after parturition. Plasma AMH concentrations in heifers were positively correlated with the total number of follicles (r = 0.647, P < 0.01) and embryos (r = 0.681, P < 0.01) recovered from the animals postpartum, when selected as donor cows, but did not correlate with the total number of transferable embryos. No difference was observed between the plasma AMH concentration at the heifer period and the postpartum period. Additionally, serum AMH concentrations of heifers weakly correlated with the number of follicles and embryos recovered by virgin flush after superovulation treatment at 13-15 months of age. Therefore, a single blood AMH concentration measurement may accelerate intensive JB cattle breeding.
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- 2020
18. The Crazy Ovary
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Danielle Monniaux, Ken McNatty, and Philippe Monget
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Ovulation ,0301 basic medicine ,reactivation ,steroidogenesis ,endocrine system ,media_common.quotation_subject ,unexpected ,Ovary ,Review ,QH426-470 ,Biology ,Luteal phase ,Bone morphogenetic protein ,03 medical and health sciences ,Oogenesis ,0302 clinical medicine ,folliculogenesis ,Follicular phase ,Genetics ,medicine ,Animals ,Humans ,Genetics (clinical) ,media_common ,030219 obstetrics & reproductive medicine ,Oocyte ,Biological Evolution ,Cell biology ,ovulation rate ,030104 developmental biology ,medicine.anatomical_structure ,primordial follicles ,Female ,ovary ,Folliculogenesis ,Corpus luteum ,crazy - Abstract
From fetal life until senescence, the ovary is an extremely active tissue undergoing continuous structural and functional changes. These ever-changing events are best summarized by a quotation attributed to Plato when describing motion in space and time—‘nothing ever is but is always becoming…’. With respect to the ovary, these changes include, at the beginning, the processes of follicular formation and thereafter those of follicular growth and atresia, steroidogenesis, oocyte maturation, and decisions relating to the number of mature oocytes that are ovulated for fertilization and the role of the corpus luteum. The aims of this review are to offer some examples of these complex and hitherto unknown processes. The ones herein have been elucidated from studies undertaken in vitro or from normal in vivo events, natural genetic mutations or after experimental inactivation of gene function. Specifically, this review offers insights concerning the initiation of follicular growth, pathologies relating to poly-ovular follicles, the consequences of premature loss of germ cells or oocytes loss, the roles of AMH (anti-Müllerian hormone) and BMP (bone morphogenetic protein) genes in regulating follicular growth and ovulation rate together with species differences in maintaining luteal function during pregnancy. Collectively, the evidence suggests that the oocyte is a key organizer of normal ovarian function. It has been shown to influence the phenotype of the adjacent somatic cells, the growth and maturation of the follicle, and to determine the ovulation rate. When germ cells or oocytes are lost prematurely, the ovary becomes disorganized and a wide range of pathologies may arise.
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- 2021
19. A bovine-specific FSH enzyme immunoassay and its application to study the role of FSH in ovarian follicle development during the postnatal period
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J. Furlong, Danielle Monniaux, Anne-Lyse Lainé, C. Laclie, Mark A. Crowe, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), School of Veterinary Medicine, University of California, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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endocrine system ,Population ,cow ,Ovary ,Biology ,SF1-1100 ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Follicular phase ,FSH ,medicine ,immunoassay ,Ovarian follicle ,education ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,030304 developmental biology ,Estrous cycle ,0303 health sciences ,education.field_of_study ,calf ,030219 obstetrics & reproductive medicine ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Radioimmunoassay ,Antral follicle ,Animal culture ,medicine.anatomical_structure ,Animal Science and Zoology ,ovary ,hormones, hormone substitutes, and hormone antagonists ,Blood sampling - Abstract
International audience; The primary aim of this study was to develop a FSH enzyme immunoassay (EIA) for the bovine species. The newly developed EIA was validated for FSH determination in bovine plasma by comparison with an existing bovine FSH radioimmunoassay. The EIA detected bovine FSH with a high sensitivity (0.1 ng/ml). Cross-reactivity of the EIA was 0.01% with bovine LH, 51% with ovine FSH
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- 2018
20. The Bone Morphogenetic Protein 15 Up-Regulates the Anti-Müllerian Hormone Receptor Expression in Granulosa Cells
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Jean-Yves Picard, J. Folch, Anthony Estienne, Stéphane Fabre, B. Lahoz, Renato Fanchin, Peggy Jarrier, Chrystèle Racine, Alice Pierre, Danielle Monniaux, Nathalie di Clemente, J.L. Alabart, U 1133, Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Diderot - Paris 7 (UPD7), Université Sorbonne Paris Cité (USPC), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unidad de Producción y Sanidad Animal, Centro de Investigacion y Technologia Agroalimentaria de Aragon (CITA), Instituto Agroalimentario de Aragón, University of Zaragoza - Universidad de Zaragoza [Zaragoza], Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Agence Nationale pour la Recherche (grant ANR-12-BSV1–0034–01 to Nathalie di Clemente, grant ANR-10-BLAN-1608–01 to Stéphane Fabre), Agence de la Biomédecine (grant to Renato Fanchin), Région Centre, INRA, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,cellule de granulosa ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Growth Differentiation Factor 9 ,Bone Morphogenetic Protein 4 ,Biochemistry ,mullerian-inhibitory substance ,Ovinos ,0302 clinical medicine ,Endocrinology ,Ovarian Follicle ,bone morphogenetic protein ,Promoter Regions, Genetic ,Regulation of gene expression ,030219 obstetrics & reproductive medicine ,Up-Regulation ,medicine.anatomical_structure ,producción y sanidad animal ,hormone antimullérienne ,Bone morphogenetic protein 4 ,Hormone receptor ,Reproductividad ,Female ,Bone Morphogenetic Protein 15 ,hormones, hormone substitutes, and hormone antagonists ,Autre (Sciences du Vivant) ,Adult ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,endocrine system ,medicine.medical_specialty ,Receptors, Peptide ,Biology ,Growth differentiation factor-9 ,Bone morphogenetic protein ,Young Adult ,03 medical and health sciences ,Internal medicine ,Anti-Müllerian hormone receptor ,medicine ,Animals ,Humans ,Ovarian follicle ,protéine morphogénétique ,Granulosa Cells ,Sheep ,Bone morphogenetic protein 15 ,urogenital system ,Biochemistry (medical) ,Genética ,030104 developmental biology ,Gene Expression Regulation ,Receptors, Transforming Growth Factor beta - Abstract
Anti-Müllerian hormone (AMH) is produced by the granulosa cells (GCs) of growing follicles and inhibits follicular development. This study aimed to investigate the regulation of the AMH-specific receptor gene (AMHR2) expression in GCs by bone morphogenetic protein 15 (BMP15), BMP4 and growth differentiation factor 9 (GDF9). Their effects on AMHR2 and AMH mRNAs were studied in luteinized human GCs (hGCs) and in ovine GCs (oGCs) from small antral follicles. The effects of BMPs on human AMHR2 and AMH promoter reporter activities were analyzed in transfected oGCs. The in vivo effect of BMP15 on GCs AMHR2 and AMH expression was investigated by using Lacaune and Rasa Aragonesa hyperprolific ewes carrying loss-of-function mutations in BMP15. mRNAs were quantified by real-time RT-PCR. Promoter reporter constructs activities were quantified by the measurement of their luciferase activity. BMP15 and BMP4 enhanced AMHR2 and AMH expression in hGCs and in oGCs whereas GDF9 had no effect. In oGCs, GDF9 increased BMP15 effect on AMH expression. Consistent with these results, BMP15 and BMP4, but not GDF9, enhanced AMHR2 promoter activity in oGCs, whereas GDF9 increased BMP15 effect on AMH promoter activity. Moreover, oGCs from both BMP15 mutant ewes had reduced AMHR2 mRNA levels but unchanged AMH expression compared to wild-type ewes. Altogether, these results suggest that the mechanisms of action of BMP15 on AMHR2 and AMH expression are different, and that by stimulating AMHR2 and AMH expression in GCs BMP15 enhances AMH inhibitory actions in GCs.
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- 2016
21. Multi-scale modelling of ovarian follicular development: From follicular morphogenesis to selection for ovulation
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Philippe Michel, Marie Postel, Danielle Monniaux, and Frédérique Clément
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0301 basic medicine ,endocrine system ,medicine.medical_specialty ,030219 obstetrics & reproductive medicine ,Cellular differentiation ,media_common.quotation_subject ,Morphogenesis ,Cell Biology ,General Medicine ,Biology ,Oocyte ,Cell biology ,03 medical and health sciences ,Follicle ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Endocrinology ,Internal medicine ,Follicular phase ,medicine ,Folliculogenesis ,Ovulation ,Germ cell ,media_common - Abstract
In this review, we present multi-scale mathematical models of ovarian follicular development that are based on the embedding of physiological mechanisms into the cell scale. During basal follicular development, follicular growth operates through an increase in the oocyte size concomitant with the proliferation of its surrounding granulosa cells. We have developed a spatio-temporal model of follicular morphogenesis explaining how the interactions between the oocyte and granulosa cells need to be properly balanced to shape the follicle. During terminal follicular development, the ovulatory follicle is selected amongst a cohort of simultaneously growing follicles. To address this process of follicle selection, we have developed a model giving a continuous and deterministic description of follicle development, adapted to high numbers of cells and based on the dynamical and hormonally regulated repartition of granulosa cells into different cell states, namely proliferation, differentiation and apoptosis. This model takes into account the hormonal feedback loop involving the growing ovarian follicles and the pituitary gland, and enables the exploration of mechanisms regulating the number of ovulations at each ovarian cycle. Both models are useful for addressing ovarian physio-pathological situations. Moreover, they can be proposed as generic modelling environments to study various developmental processes and cell interaction mechanisms.
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- 2016
22. 126 Regulation of initiation of follicle growth and dynamics of early follicular development in the sheep
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Yann Locatelli, Philippe Monget, Frédérique Clément, Danielle Monniaux, Rozenn Dalbies-Tran, V. Cadoret, Stéphane Fabre, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Mathematical and Mechanical Modeling with Data Interaction in Simulations for Medicine (M3DISIM), Laboratoire de mécanique des solides (LMS), École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École polytechnique (X)-Inria Saclay - Ile de France, École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), École polytechnique (X)-Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Mines Paris - PSL (École nationale supérieure des mines de Paris), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), and Université de Toulouse (UT)-Université de Toulouse (UT)
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endocrine system ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,Ovarian Cortex ,General Medicine ,Biology ,Oocyte ,Antral follicle ,follicle ,Cell biology ,Abstracts ,Follicle ,medicine.anatomical_structure ,Theca ,Follicular phase ,Genetics ,medicine ,Animal Science and Zoology ,ovary ,Folliculogenesis ,oocyte ,Food Science ,Extracellular matrix organization - Abstract
Notice à reprendre pas de clé UT au 14 Janvier 2018; International audience; Primordial follicles embedded in the ovarian cortex are the source of developing follicles. Follicle growth activationand development up to the small antral follicle stage are controlled by cell interactions. The sheep ovary offersan appropriate non-rodent model to study these interactions, thanks to the development of in vitro and in vivoexperimental approaches, ex vivo molecular analyses and in silico mathematical modeling. Each primordial follicle, composed of an oocyte surrounded by a single layer of quiescent granulosa cells, relies on nutrients and growth factors supplied by the surrounding stroma of connective tissue. In vitro cultures of ovarian cortex have shown that primordial follicles are activated by the lifting of mechanisms maintaining quiescence, some of them involving AMH secreted by already growing follicles. Afterwards, follicle development is supported by a finely tuned molecular dialog between the growing oocyte and proliferating granulosa cells. The isolation of preantral follicles and their development in vitro as individual follicles perturb this dialog, leading to an acceleration of follicular maturation. In vivo, mutations in the oocyte factors BMP15, GDF9 or their receptor BMPR1B also impair this dialog, leading to an imbalance between oocyte growth and cell proliferation, which can be reproduced by models for cell dynamics. During the growth of preantral follicles, the recruitment and differentiation of theca cells from the ovarian stroma provide them with a structural and vascularized support. In vivo exposure of sheep fetalovaries to testosterone imprints the stroma cells so that the expression of genes involved in extracellular matrixorganization and cell-cell adhesion is affected in theca at adulthood; this leads to a lower ovarian tissue rigiditythat can account for the accelerated follicle growth observed in androgenized ewes. A better knowledge ofcell interactions during early follicular development will help to improve the biotechnology methods of fertilitypreservation.
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- 2018
23. Advances in the molecular pathophysiology, genetics, and treatment of primary ovarian insufficiency
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Danielle Monniaux, Andres Salumets, Ilpo Huhtaniemi, Micheline Misrahi, Peter Wieacker, Outi Hovatta, Katarzyna Jarzabek, Juha S. Tapanainen, Antonio La Marca, Jenny A. Visser, Slawomir Wolczynski, Reiner A. Veitia, Luca Persani, Triin Laisk-Podar, Abdelkader Heddar, Gabriel Livera, Imperial College London, Nova Southeastern University (NSU), Karolinska Institutet [Stockholm], Università degli Studi di Modena e Reggio Emilia (UNIMORE), Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Milano [Milano] (UNIMI), Istituto Auxologico Italiano, Université Paris-Sud - Paris 11 (UP11), Polish Academy of Sciences (PAN), Competence Centre on Health Technologies, Partenaires INRAE, University of Tartu, University of Helsinki, Department of Obstetrics and Gynecology, University of Oulu-Institute of Clinical Medicine, Université Paris Diderot - Paris 7 (UPD7), Erasmus University Rotterdam, University Hospital Münster - Universitaetsklinikum Muenster [Germany] (UKM), Medical University of Bialystok, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,Adult ,transfer-rna synthetase ,fsh receptor ,chromosomal instability ,DNA repair ,Endocrinology, Diabetes and Metabolism ,[SDV]Life Sciences [q-bio] ,perrault syndrome ,Biology ,Primary Ovarian Insufficiency ,in-vitro ,hypergonadotropic hypogonadism ,of-function mutations ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Humans ,genetics ,[INFO]Computer Science [cs] ,Fertility preservation ,hearing-loss ,Ovarian reserve ,Exome ,Gene ,exome ,in vitro activation of dormant follicles ,meiosis genes ,ovary ,primary ovarian insufficiency ,Genetic association ,Genetics ,follicle-stimulating-hormone ,030219 obstetrics & reproductive medicine ,Genetic heterogeneity ,High-Throughput Nucleotide Sequencing ,3. Good health ,Review article ,Diabetes and Metabolism ,030104 developmental biology ,Mutation ,Female ,primordial follicle ,Genome-Wide Association Study - Abstract
International audience; Primary ovarian insufficiency (POI) affects similar to 1% of women before 40 years of age. The recent leap in genetic knowledge obtained by next generation sequencing (NGS) together with animal models has further elucidated its molecular pathogenesis, identifying novel genes/pathways. Mutations of > 60 genes emphasize high genetic heterogeneity. Genome-wide association studies have revealed a shared genetic background between POI and reproductive aging. NGS will provide a genetic diagnosis leading to genetic/therapeutic counseling: first, defects in meiosis or DNA repair genes may predispose to tumors; and second, specific gene defects may predict the risk of rapid loss of a persistent ovarian reserve, an important determinant in fertility preservation. Indeed, a recent innovative treatment of POI by in vitro activation of dormant follicles proved to be successful.
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- 2018
24. Dysregulation of the anti-mullerian hormone system by steroids in women with polycystic ovary syndrome
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Joëlle Cohen-Tannoudji, Hady El Hachem, Frank Giton, Nathalie di Clemente, Michael Grynberg, Danielle Monniaux, Alice Pierre, Joëlle Taieb, Salma Touleimat, Renato Fanchin, Chrystèle Racine, Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), U 1133, Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de Tours (UT)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Institut National de la Recherche Agronomique (INRA)
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Adult ,Anti-Mullerian Hormone ,0301 basic medicine ,medicine.medical_specialty ,endocrine system ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,Receptors, Peptide ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Estrogen receptor ,Context (language use) ,Biochemistry ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Internal medicine ,Follicular phase ,medicine ,Estrogen Receptor beta ,Humans ,Testosterone ,Granulosa Cells ,030219 obstetrics & reproductive medicine ,Estradiol ,biology ,urogenital system ,Biochemistry (medical) ,Estrogen Receptor alpha ,Dihydrotestosterone ,Anti-Müllerian hormone ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Polycystic ovary ,female genital diseases and pregnancy complications ,Androgen receptor ,030104 developmental biology ,Follicular Phase ,Gene Expression Regulation ,Receptors, Androgen ,Case-Control Studies ,biology.protein ,Female ,Receptors, Transforming Growth Factor beta ,hormones, hormone substitutes, and hormone antagonists ,Polycystic Ovary Syndrome ,Hormone - Abstract
International audience; Context: Anti-Mullerian hormone (AMH) and AMH type II receptor (AMHR2) are overexpressed in granulosa cells (GCs) from women with polycystic ovary syndrome (PCOS), the most common cause of female infertility. Objective: The aim of the study was to compare the regulation of the AMH/AMHR2 system by 5adihydrotestosterone (5a-DHT) and estradiol (E2) in GCs from control subjects and women with PCOS. Design, Setting, Patients: Experiments were performed on follicular fluids (FF) and GCs from women undergoing in vitro fertilization. Main Outcome Measures: FF steroid levels were measured by mass spectrometry, and messenger RNA (mRNA) accumulation was quantified by reverse transcription real-time polymerase chain reaction. Results: Total testosterone (T), free T, and 5 alpha-DHT FF levels were significantly higher (P < 0.001) in women with PCOS than in controls. However, E2 and sex hormone-binding globulin concentrations were comparable between the two groups. In GCs from control women, the AMH and AMHR2 expression were not affected by 5a-DHT treatment, whereas AMH mRNA levels were upregulated by 5a-DHT in GCs from patients with PCOS (2.3-fold, P < 0.01) overexpressing the androgen receptor (1.4-fold, P < 0.05). E2 downregulated the AMH and AMHR2 expression in GCs from control women (1.4-fold, P < 0.001 and 1.8-fold, P < 0.01, respectively) but had no effect on these genes in GCs from women with PCOS. This differential effect of E2 was associated with a higher estrogen receptor 1 expression in GCs from women with PCOS (1.9-fold, P < 0.05). Conclusions: In GCs from women with PCOS, the regulation of AMH and AMHR2 expression is altered in a way that promotes the overexpression of the AMH/AMHR2 system, and could contribute to the follicular arrest observed in these patients.
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- 2017
25. Molecular evidence that follicle development is accelerated in vitro compared to in vivo
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D. Royère, F. Guerif, Philippe Monget, Yann Locatelli, Peggy Jarrier, Agnès Bonnet, Virginie Maillard, Véronique Cadoret, Danielle Monniaux, Cynthia Frapsauce, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Med & Biol Reprod CECOS, CHU Bretonneau, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,Embryology ,endocrine system ,In Vitro Oocyte Maturation Techniques ,[SDV]Life Sciences [q-bio] ,mammalian oogenesis ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Follicular phase ,granusola-cell ,cultured follicle ,medicine ,Ovarian follicle ,Granulosa cell proliferation ,anti-mullerian hormone ,Granulosa cell differentiation ,030219 obstetrics & reproductive medicine ,Chemistry ,secondary follicle ,Obstetrics and Gynecology ,Cell Biology ,Antral follicle ,Estradiol secretion ,messenger-rna expression ,030104 developmental biology ,medicine.anatomical_structure ,Reproductive Medicine ,gene expression ,quantitative expression ,preantral follicle ,Folliculogenesis ,ovarian-follicle - Abstract
In this study, we systematically compared the morphological, functional and molecular characteristics of granulosa cells and oocytes obtained by a three-dimensionalin vitromodel of ovine ovarian follicular growth with those of follicles recoveredin vivo. Preantral follicles of 200 µm diameter were recovered and cultured up to 950 µm over a 20-day period. Compared within vivofollicles, thein vitroculture conditions maintained follicle survival, with no difference in the rate of atresia. However, thein vitroconditions induced a slight decrease in oocyte growth rate, delayed antrum formation and increased granulosa cell proliferation rate, accompanied by an increase and decrease inCCND2andCDKN1AmRNA expression respectively. These changes were associated with advanced granulosa cell differentiation in early antral follicles larger than 400 µm diameter, regardless of the presence or absence of FSH, as indicated by an increase in estradiol secretion, together with decreased AMH secretion and expression, as well as increased expression ofGJA1,CYP19A1,ESR1,ESR2,FSHR,INHA,INHBA,INHBBandFST. There was a decrease in the expression of oocyte-specific molecular markersGJA4,KIT,ZP3,WEE2andBMP15 in vitrocompared to thatin vivo. Moreover, a higher percentage of the oocytes recovered from cultured follicles 550 to 950 µm in diameter was able to reach the metaphase II meiosis stage. Overall, thisin vitromodel of ovarian follicle development is characterized by accelerated follicular maturation, associated with improved developmental competence of the oocyte, compared to follicles recoveredin vivo.
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- 2017
26. Differentially expressed genes and gene networks involved in pig ovarian follicular atresia
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Magali SanCristobal, Agnès Bonnet, Julien Sarry, Elena Terenina, Danielle Monniaux, Philippe Monget, Florence Gondret, Florence Vignoles, Gwenola Tosser-Klopp, Christèle Robert-Granié, Stéphane Fabre, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), INPT-ENSAT - INPT-ENVT, UMR 1388 GenPhySE Génétique Physiologie et Systèmes d'Elevage. Centre de recherche Toulouse, Institut National de la Recherche Agronomique (INRA), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, French Agence Nationale de la Recherche GENOVUL project (ANR-05-GANI-001-01), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,pig ,Candidate gene ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,Physiology ,Granulosa cell ,Sus scrofa ,Down-Regulation ,Apoptosis ,atresia ,Biology ,atresie folliculaire ,03 medical and health sciences ,Ovarian Follicle ,folliculogenesis ,Genetics ,medicine ,Animals ,Cluster Analysis ,Gene Regulatory Networks ,porcin ,Ovarian follicle ,genes ,upstream regulators ,Gene Expression Profiling ,Follicular atresia ,gène ,ovaire ,biomarkers ,medicine.disease ,Up-Regulation ,Cell biology ,Gene expression profiling ,INHBB ,Gene Ontology ,030104 developmental biology ,medicine.anatomical_structure ,Atresia ,gene expression ,Female ,ovary ,Folliculogenesis ,follicular atresia ,Autre (Sciences du Vivant) ,Signal Transduction - Abstract
Ovarian folliculogenesis corresponds to the development of follicles leading to either ovulation or degeneration, this latter process being called atresia. Even if atresia involves apoptosis, its mechanism is not well-understood. The objective of this project was to analyse global gene expression in pig granulosa cells of ovarian follicles during atresia. The transcriptome analysis was performed using 9216 cDNAs microarray to identify gene networks and candidate genes involved in pig ovarian follicular atresia. One thousand six hundred and eighty four significantly regulated genes were differentially regulated between small healthy follicles and small atretic follicles. Among them, two hundred and eighty seven genes had a fold-change higher than 2 between the two follicle groups. Eleven genes (DKK3, GADD45A, CAMTA2, CCDC80, DAPK2, ECSIT, MSMB, NUPR1, RUNX2, SAMD4A, and ZNF628) having a fold-change higher than 5 between groups could likely serve as markers of follicular atresia. Moreover, automatic confrontation of deregulated genes with literature data enlightened 93 genes as regulatory candidates of pig granulosa cell atresia. Among these genes known to be inhibitors of apoptosis, stimulators of apoptosis or tumor suppressors INHBB, HNF4, CLU, different interleukins (IL5, IL24), TNF-associated receptor (TNFR1), and cytochrome-c oxidase (COX) were suggested as playing an important role in porcine atresia. Present study also enlists key upstream regulators in follicle atresia based on our results and on a literature review. The novel gene candidates and gene networks identified in the current study lead to a better understanding of the molecular regulation of ovarian follicular atresia.
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- 2017
27. Molecular evidence that follicle development is accelerated
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Véronique, Cadoret, Cynthia, Frapsauce, Peggy, Jarrier, Virginie, Maillard, Agnès, Bonnet, Yann, Locatelli, Dominique, Royère, Danielle, Monniaux, Fabrice, Guérif, and Philippe, Monget
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Granulosa Cells ,Oogenesis ,Sheep ,Ovarian Follicle ,Gene Expression Profiling ,Oocytes ,Animals ,Female ,In Vitro Techniques ,Biomarkers ,Cells, Cultured ,In Vitro Oocyte Maturation Techniques - Abstract
In this study, we systematically compared the morphological, functional and molecular characteristics of granulosa cells and oocytes obtained by a three-dimensional
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- 2016
28. Regulation of Anti-Müllerian Hormone Production in the Cow: A Multiscale Study at Endocrine, Ovarian, Follicular, and Granulosa Cell Levels1
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Martine Bontoux, Claire Médigue, Danielle Monniaux, Peggy Jarrier, Stéphane Fabre, Charlène Rico, and Frédérique Clément
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endocrine system ,medicine.medical_specialty ,endocrine system diseases ,Granulosa cell ,media_common.quotation_subject ,Ovary ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Follicular phase ,medicine ,Ovarian follicle ,Ovulation ,030304 developmental biology ,media_common ,2. Zero hunger ,Estrous cycle ,0303 health sciences ,030219 obstetrics & reproductive medicine ,urogenital system ,Anti-Müllerian hormone ,Cell Biology ,General Medicine ,female genital diseases and pregnancy complications ,Embryo transfer ,medicine.anatomical_structure ,Endocrinology ,Reproductive Medicine ,biology.protein ,hormones, hormone substitutes, and hormone antagonists - Abstract
Anti-Mullerian hormone (AMH) is an endocrine marker that can help predict superovulatory responses to treatments administered to cows for embryo production. However, the optimal time of the estrous cycle at which a blood test should be performed for a highly reliable prognosis has not yet been established. Moreover, little is known about the regulation of AMH production. To answer these questions, a study was designed to investigate the regulation of AMH production in cows selected for their high or low ovulatory responses to superovulation. At the granulosa cell level, AMH production was inhibited by follicle-stimulating hormone but enhanced by bone morphogenetic proteins. At the follicular level, the expression of AMH within the follicle was dependent on the stage of follicular development. At the ovarian level, the size of the pool of small antral growing follicles determined ovarian AMH production. At the endocrine level, AMH followed a specific dynamic profile during the estrous cycle, which occurred independently of the follicular waves of terminal follicular development. Cows selected for their high or low responses to superovulation did not differ in the regulation of AMH production, but cows with higher responses had higher plasma AMH concentrations throughout the cycle. The optimal period of the estrous cycle at which to measure AMH concentrations with the aim of selecting the best cows for embryo production was found to be at estrus and after Day 12 of the cycle. Based on this multiscale study, we propose a model that integrates the different regulatory levels of AMH production.
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- 2011
29. Développement folliculaire ovarien et ovulation chez les mammifères
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Danielle Monniaux, Frédérique Clément, Svetlana Uzbekova, Nadine Gérard, Joëlle Dupont, Stéphane Fabre, Pascal Mermillod, Alain Caraty, Philippe Monget, Rozenn Dalbies-Tran, Unité Pluri-espèces d'Expérimentation Animale en Physiologie de la Reproduction et des Comportements (TOURS UPEA PRC), Institut National de la Recherche Agronomique (INRA), SIgnals and SYstems in PHysiology & Engineering (SISYPHE), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de Recherche en Informatique et en Automatique (Inria)
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[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,endocrine system ,0303 health sciences ,folliculogénèse ,030219 obstetrics & reproductive medicine ,ovogénèse ,ovaire ,reproduction animale ,mammifère ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,ovocyte ,follicule ovarien ,Agricultural sciences ,03 medical and health sciences ,0302 clinical medicine ,femelle ,ovulation ,Sciences agricoles ,030304 developmental biology - Abstract
Cette revue présente l’état des connaissances sur la folliculogenèse et l’ovulation chez les mammifères. La folliculogenèse basale est une phase de croissance folliculaire lente, au cours de laquelle l’ovocyte acquiert la compétence méiotique. La folliculogenèse terminale est une phase de développement rapide, au cours de laquelle le follicule ovulatoire est sélectionné et termine sa maturation, tandis que l’ovocyte acquiert la compétence au développement. La revue décrit les différents changements fonctionnels qui s’opèrent dans le follicule et l’ovocyte au cours de ces deux phases, ainsi que les mécanismes qui les régulent et qui déterminent le quota ovulatoire caractéristique de chaque espèce ou race. Les facteurs-clés identifiés de la folliculogenèse sont les BMP, le KITLG et l’AMH pour le démarrage de croissance folliculaire, l’IGF et l’insuline pour la transition entre folliculogenèse basale et terminale, la FSH pour le début de la folliculogenèse terminale et la sélection des follicules ovulatoires, et la LH pour le développement folliculaire final, la maturation ovocytaire et l’ovulation. L’ovulation est déclenchée par une décharge préovulatoire de LH qui induit la maturation du complexe cumulus-ovocyte dans le follicule ovulatoire, suivie de la rupture de la paroi folliculaire et de la formation du corps jaune. La folliculogenèse ovarienne est contrôlée par des facteurs externes, tels que la photopériode et la nutrition, et c’est essentiellement le système hypothalamo-hypophysaire qui intègre les signaux endocriniens et environnementaux. Des modifications de l’apport alimentaire, relayées par des variations de signaux hormonaux et métaboliques, modulent l’activité de l’axe hypothalamo-hypophyso-ovarien à ses différents étages., This review is a state of the art on follicullogenesis and ovulation in mammals. Basal folliculogenesis is a phase of slow follicular growth, during which the oocyte acquires meiotic competence. Terminal folliculogenesis is a phase of rapid development, during which the ovulatory follicle is selected and completes its maturation while the oocyte acquires developmental competence. This review describes the different functional changes occurring within follicles and oocytes throughout these two phases, the underlying regulatory mechanisms and the mechanisms that determine natural ovulation rate in different species and breeds. Known key factors for folliculogenesis are BNTP, KITLG and AMH for the initiation of follicular growth, IGF and insulin for transition between basal and terminal folliculogenesis, FSH for the outset of terminal folliculogenesis and the selection of ovulatory follicles, and LH for final follicular development, oocyte maturation and ovulation. Ovulation is triggered by a preovulatory LH surge that induces maturation of the cumulus oocyte complex within the ovulatory follicle, rupture of the follicular wall and corpus luteum formation. Ovarian folliculogenesis is controlled by external factors such as photoperiod and nutrition, and the hypothalamo-pituitary system integrates endocrine and environmental signals. Nutritional supply, relayed by hormonal and metabolic signals, can modulate both the hypothalamo-pituitary and ovarian activity.
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- 2009
30. Inhibitors of c-Jun phosphorylation impede ovine primordial follicle activation
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Yann Locatelli, Michael J. Bertoldo, Svetlana Uzbekova, Danielle Monniaux, Laure Calais, Nicolas Duffard, Pascal Mermillod, Guillaume Tsikis, Sabine Alves, Jérémy Bernard, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), School of Women's and Children's Health, Discipline of Obstetrics and Gynaecology, University of New South Wales [Sydney] (UNSW), Réserve de la Haute Touche, Direction générale déléguée aux musées et aux jardins botaniques et zoologiques (DGD.MJZ), Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN), Région Centre (CRYOVAIRE, Grant number #320000268), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Direction des Jardins botaniques et zoologiques, Muséum national d'Histoire naturelle (MNHN), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,Anti-Mullerian Hormone ,Embryology ,medicine.medical_specialty ,endocrine system ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,fertility preservation ,Proto-Oncogene Proteins c-jun ,c-Jun-N-terminal kinase ,03 medical and health sciences ,Follicle ,0302 clinical medicine ,Ovarian Follicle ,Internal medicine ,Genetics ,medicine ,Animals ,Ovarian follicle ,Phosphorylation ,Molecular Biology ,Anthracenes ,030219 obstetrics & reproductive medicine ,Sheep ,biology ,Kinase ,primordial follicle activation ,Ovary ,Obstetrics and Gynecology ,Anti-Müllerian hormone ,Foxo3a ,in vitro ovarian tissue culture ,Cell Biology ,Hair follicle ,Proliferating cell nuclear antigen ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,Apoptosis ,biology.protein ,Female ,Folliculogenesis ,Follicle Stimulating Hormone ,Developmental Biology ,Signal Transduction - Abstract
STUDY HYPOTHESIS Is the c-Jun-N-terminal kinase (JNK) pathway implicated in primordial follicle activation? STUDY FINDING Culture of ovine ovarian cortex in the presence of two different c-Jun phosphorylation inhibitors impeded pre-antral follicle activation. WHAT IS KNOWN ALREADY Despite its importance for fertility preservation therapies, the mechanisms of primordial follicle activation are poorly understood. Amongst different signalling pathways potentially involved, the JNK pathway has been previously shown to be essential for cell cycle progression and pre-antral follicle development in mice. STUDY DESIGN, SAMPLES/MATERIALS, METHODS Ovine ovarian cortex pieces were cultured with varying concentrations of SP600125, JNK inhibitor VIII or anti-Mullerian hormone (AMH) in the presence of FSH for 9 days. Follicular morphometry and immunohistochemistry for proliferating cell nuclear antigen (PCNA), apoptosis and follicle activation (Foxo3a) were assessed. MAIN RESULTS AND THE ROLE OF CHANCE Inhibition of primordial follicle activation occurred in the presence of SP600125, JNK inhibitor VIII and AMH when compared with controls (all P < 0.05) after 2 days of culture. However, only in the highest concentrations used was the inhibition of activation associated with induction of follicular apoptosis (P < 0.05). In growing follicles, PCNA antigen expression was reduced when the JNK inhibitors or AMH were used (P < 0.05 versus control), indicating reduced proliferation of the somatic compartment. LIMITATIONS, REASONS FOR CAUTION Although we evaluated the effects of inhibition of c-Jun phosphorylation on primordial follicle development, we did not determine the cellular targets and mechanism of action of the inhibitors. WIDER IMPLICATIONS OF THE FINDINGS These results are the first to implicate the JNK pathway in primordial follicle activation and could have significant consequences for the successful development of fertility preservation strategies and our understanding of primordial follicle activation. LARGE SCALE DATA n/a. STUDY FUNDING AND COMPETING INTERESTS Dr Michael J. Bertoldo and the laboratories involved in the present study were supported by a grant from 'Region Centre' (CRYOVAIRE, Grant number #320000268). There are no conflicts of interest to declare.
- Published
- 2015
31. Anti-Müllerian hormone regulation by the bone morphogenetic proteins in the sheep ovary: deciphering a direct regulatory pathway
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Stéphane Fabre, Anthony Estienne, Nathalie di Clemente, Peggy Jarrier, J. Y. Picard, Camille Mansanet, Danielle Monniaux, Alice Pierre, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Université Paris Diderot - Paris 7 (UPD7), Physiologie de l'Axe Gonadotrope (PAG U1133), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], AMHAROC (ANR-12-BSV1– 0034-02), Région Centre, INRA, Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Anti-Mullerian Hormone ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,endocrine system ,medicine.medical_specialty ,endocrine system diseases ,Genotype ,Granulosa cell ,Ovary ,Bone Morphogenetic Protein 4 ,Biology ,Bone morphogenetic protein ,Transfection ,Transactivation ,Endocrinology ,Internal medicine ,Follicular phase ,medicine ,Animals ,Humans ,Bone morphogenetic protein receptor ,RNA, Messenger ,Ovarian follicle ,Promoter Regions, Genetic ,Bone Morphogenetic Protein Receptors, Type I ,Cells, Cultured ,Binding Sites ,Granulosa Cells ,Sheep ,urogenital system ,Anti-Müllerian hormone ,female genital diseases and pregnancy complications ,medicine.anatomical_structure ,Gene Expression Regulation ,Mutation ,biology.protein ,Female ,hormones, hormone substitutes, and hormone antagonists - Abstract
Remerciements : Unité Expérimentale du Domaine du Merle de l'UMR0868 SELMET Systèmes d'Elevage Méditerranéens et Tropicaux, SupAgro Montpellier; In the ovary, anti-Müllerian hormone (AMH) is produced by the granulosa cells of growing follicles and can modulate the recruitment of primordial follicles and the follicle stimulating hormone (FSH)-dependent development of follicles. However, the regulation of its production remains poorly understood. Recently, a stimulating effect of the bone morphogenetic proteins (BMPs) on AMH production by granulosa cells has been shown in vitro, but the molecular mechanisms implicated in this regulation and its physiological importance in ovarian function have not yet been established. In the hyperprolific Booroola ewes carrying the FecB(B) partial loss-of-function mutation in the gene encoding the FecB/BMPR1B receptor, the granulosa cells of antral follicles expressed and secreted low AMH amounts, resulting in low AMH concentrations in blood, despite high numbers of AMH-secreting follicles in ovaries. The presence of the FecB(B) mutation impaired the granulosa cell response to the stimulating action of BMP4 on AMH production, indicating a crucial role of the BMPR1B receptor in AMH regulation. In ovine granulosa cells, BMP4 enhanced the transcriptional activity of the human AMH promoter and this action depended on the presence of SMAD1, acting on a promoter sequence located between -423 and -202 bp upstream of the AMH transcription start site. SMAD1 and SF1 acted in concert to mediate BMP4 action on the AMH promoter. Among the two SF1 binding sites present on the AMH promoter, the most proximal site, located at -92 bp upstream of the AMH transcription start site, was found to be critical for ensuring the response of the AMH promoter to BMP4. In conclusion, AMH could mediate the actions of BMPs in regulating follicular development and contributing to the determination of ovulation numbers. A molecular model of regulation of the AMH promoter transactivation by BMP signaling is proposed.
- Published
- 2014
32. The Booroola mutation in sheep is associated with an alteration of the bone morphogenetic protein receptor-IB functionality
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Philippe Monget, Danielle Monniaux, Stéphane Fabre, P Mulsant, Alice Pierre, J Pohl, C. Pisselet, F Lecerf, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
medicine.medical_specialty ,Genotype ,[SDV]Life Sciences [q-bio] ,Endocrinology, Diabetes and Metabolism ,Bone Morphogenetic Protein 4 ,Protein Serine-Threonine Kinases ,Biology ,Transfection ,Bone morphogenetic protein ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Growth Differentiation Factor 5 ,Transforming Growth Factor beta ,Internal medicine ,medicine ,Animals ,Humans ,Receptors, Growth Factor ,[INFO]Computer Science [cs] ,Bone morphogenetic protein receptor ,Receptor ,Bone Morphogenetic Protein Receptors, Type I ,Cells, Cultured ,Progesterone ,ComputingMilieux_MISCELLANEOUS ,Inhibin-beta Subunits ,030304 developmental biology ,Analysis of Variance ,0303 health sciences ,Granulosa Cells ,Sheep ,030219 obstetrics & reproductive medicine ,Progesterone secretion ,Activins ,BMPR2 ,BMPR1B ,Bone morphogenetic protein 4 ,Bone Morphogenetic Proteins ,Female ,Cell Division ,Signal Transduction - Abstract
The hyperprolificacy phenotype of Booroola ewes is due to the presence of the FecB(B) allele at the FecB locus, recently identified as a single amino acid substitution (Q249R) in the bone morphogenetic protein (BMP) type-IB receptor (BMPR1B), and is associated with a more precocious differentiation of ovarian granulosa cells (GCs). To evaluate the consequences of the Booroola mutation on BMPR1B functions, the action of ligands of the transforming growth factor-beta (TGFbeta)/BMP family that act through (growth and differentiation factor-5, BMP-4) or independently of (activin A, TGFbeta-1) BMPR1B were studied on primary cultures of GCs from homozygous FecB(+) and FecB(B) ewes. All the tested TGFbeta/BMP family ligands inhibited progesterone secretion by FecB(+) GCs. Those inhibitory effects were lower for GCs from preovulatory (5-7 mm diameter) than from small antral follicles (1-3 mm diameter). The presence of the Booroola mutation was associated with a 3- to 4-fold (P
- Published
- 2003
33. Contribution of a mathematical modelling approach to the understanding of the ovarian function
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Frédérique Clément, Danielle Monniaux, Jean-Christophe Thalabard, and Daniel Claude
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Ovulation ,endocrine system ,medicine.medical_specialty ,media_common.quotation_subject ,Granulosa cell ,Cellular differentiation ,Follicular Atresia ,Apoptosis ,Biology ,Models, Biological ,Follicular cell ,General Biochemistry, Genetics and Molecular Biology ,Ovarian Follicle ,Internal medicine ,Follicular phase ,medicine ,Animals ,Ovarian follicle ,media_common ,Granulosa Cells ,Sheep ,General Immunology and Microbiology ,Follicular atresia ,Cell Cycle ,Ovary ,Cell Differentiation ,General Medicine ,Cell biology ,Endocrinology ,medicine.anatomical_structure ,Pituitary Gland ,Female ,Folliculogenesis ,Follicle Stimulating Hormone ,General Agricultural and Biological Sciences ,Cell Division ,Mathematics - Abstract
The biological meaning of folliculogenesis is to free fertilisable oocytes at the time of ovulation. We approached the study of the control of follicular development at the level of follicular granulosa cells, on the experimental as well as mathematical modelling grounds. We built a mathematical model allowing for the processes of proliferation, differentiation and apoptosis. State variables correspond to the numbers of cells undergoing these different processes, while control variables correspond to the cellular transition rates. The model results raised the notion of proliferative resources, which leads to consider the optimal management of these resources and has motivated the settling of an experiment investigating the changes in the growth fraction within the granulosa throughout terminal development. We are now investigating the way gonadotrophins, and especially FSH, operate on granulosa cells, in order to account for the hormonal control of the divergent commitment of granulosa cells towards either proliferation, differentiation or apoptosis. We are thus focusing on the dynamics of cAMP production, which appears to be a keypoint in FSH signal transduction.
- Published
- 2002
34. Progesterone improves the maturation of male-induced preovulatory follicles in anoestrous ewes
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Pascal Papillier, Jean-Luc Touzé, Maria-Teresa Pellicer-Rubio, Didier Lomet, Achraf Adib, Sandrine Freret, Anthony Estienne, Danielle Monniaux, Lionel Lardic, Didier Chesneau, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA)
- Subjects
Male ,Vascular Endothelial Growth Factor A ,Embryology ,Time Factors ,[SDV]Life Sciences [q-bio] ,Anestrus ,Follicle-stimulating hormone ,[SCCO]Cognitive science ,Endocrinology ,Ovarian Follicle ,Follicular phase ,Progesterone ,media_common ,seasonal reproduction ,Ultrasonography ,0303 health sciences ,Estradiol ,Obstetrics and Gynecology ,Steroid 17-alpha-Hydroxylase ,04 agricultural and veterinary sciences ,Receptors, LH ,medicine.anatomical_structure ,Theca ,Receptors, FSH ,Female ,Seasons ,Luteinizing hormone ,Corpus luteum ,Ovulation ,medicine.medical_specialty ,endocrine system ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,Reproductive Techniques, Assisted ,media_common.quotation_subject ,Biology ,03 medical and health sciences ,Internal medicine ,male effect ,medicine ,Animals ,Inhibins ,Cholesterol Side-Chain Cleavage Enzyme ,RNA, Messenger ,Ovarian follicle ,030304 developmental biology ,Estrous cycle ,Sheep ,0402 animal and dairy science ,oestrus cycle ,Cell Biology ,Fertility Agents, Female ,Luteinizing Hormone ,Phosphoproteins ,040201 dairy & animal science ,Reproductive Medicine ,Gene Expression Regulation ,follicular maturation ,Follicle Stimulating Hormone - Abstract
The first ovulation induced by male effect in sheep during seasonal anoestrus usually results in the development of a short cycle that can be avoided by progesterone priming before ram introduction. In elucidating the involvement of the hypothalamic–pituitary–gonadal axis in the occurrence of short cycles, the effects of progesterone and the time of anoestrus on the development of male-induced preovulatory follicles were investigated in anoestrous ewes using morphological, endocrine and molecular approaches. Ewes were primed with progesterone for 2 (CIDR2) or 12 days (CIDR12) and untreated ewes used as controls during early (April) and late (June) anoestrus. The duration of follicular growth and the lifespan of the male-induced preovulatory follicles were prolonged by ∼1.6 days in CIDR12 ewes compared with the controls. These changes were accompanied by a delay in the preovulatory LH and FSH surges and ovulation. Intra-follicular oestradiol concentration and mRNA levels ofLHCGRandSTARin the granulosa and theca cells of the preovulatory follicles were higher in CIDR12 ewes than the control ewes. The expression of mRNA levels ofCYP11A1andCYP17A1also increased in theca cells of CIDR12 ewes. CIDR2 ewes gave intermediate results. Moreover, ewes ovulated earlier in June than in April, without changes in the duration of follicular growth, but these effects were unrelated to the lifespan of corpus luteum. Our results give the first evidence supporting the positive effect of progesterone priming on the completion of growth and maturation of preovulatory follicles induced by male effect in seasonal anoestrous ewes, thereby preventing short cycles.
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- 2014
35. The Ovarian Reserve of Primordial Follicles and the Dynamic Reserve of Antral Growing Follicles: What Is the Link?1
- Author
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Rozenn Dalbies-Tran, Anthony Estienne, Stéphane Fabre, Philippe Monget, Frédérique Clément, Camille Mansanet, and Danielle Monniaux
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endocrine system ,medicine.medical_specialty ,media_common.quotation_subject ,Ovary ,Reproductive technology ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Follicular phase ,medicine ,Ovarian reserve ,Ovulation ,030304 developmental biology ,media_common ,0303 health sciences ,030219 obstetrics & reproductive medicine ,Anti-Müllerian hormone ,Cell Biology ,General Medicine ,Antral follicle ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,biology.protein ,Folliculogenesis - Abstract
The growing follicles develop from a reserve of primordial follicles constituted early in life. From this pre-established reserve, a second ovarian reserve is formed, which consists of gonadotropin-responsive small antral growing follicles and is a dynamic reserve for ovulation. Its size, evaluated by direct antral follicular count or endocrine markers, determines the success of assisted reproductive technologies in humans and embryo production biotechnologies in animals. Strong evidence indicates that these two reserves are functionally related. The size of both reserves appears to be highly variable between individuals of similar age, but the equilibrium size of the dynamic reserve in adults seems to be specific to each individual. The dynamics of both follicular reserves appears to result from the fine tuning of regulations involving two main pathways, the phosphatase and tensin homolog (PTEN)/phosphatidylinositol-3 kinase (PI3K)/3-phosphoinositide-dependent protein kinase-1 (PDPK1)/v-akt murine thymoma viral oncogene homolog 1 (AKT1) and the bone morphogenetic protein (BMP)/anti-Mullerian hormone (AMH)/SMAD signaling pathways. Mutations in genes encoding the ligands, receptors, or signaling effectors of these pathways can accelerate or modulate the exhaustion rate of the ovarian reserves, causing premature ovarian insufficiency (POI) or increase in reproductive longevity, respectively. With female aging, the decline in primordial follicle numbers parallels the decrease in the size of the dynamic reserve of small antral follicles and the deterioration of oocyte quality. Recent progress in our knowledge of signaling pathways and their environmental and hormonal control during adult and fetal life opens new perspectives to improve the management of the ovarian reserves.
- Published
- 2014
36. Mathematical model of FSH-induced cAMP production in ovarian follicles
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Daniel Claude, Danielle Monniaux, Jean-Christophe Thalabard, Kate Hardy, John Stark, Stephen Franks, Frédérique Clément, Parameter estimation and modeling in heterogeneous media (ESTIME), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), University College of London [London] (UCL), Imperial College London, Université Paris Descartes - Paris 5 (UPD5), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Physiology ,Endocrinology, Diabetes and Metabolism ,follicule ovarien ,Adenylyl cyclase ,chemistry.chemical_compound ,Follicle-stimulating hormone ,0302 clinical medicine ,Ovarian Follicle ,HORMONE GONADOTROPE ,AMP-C ,FSH ,Cyclic AMP ,Phosphorylation ,Receptor ,ComputingMilieux_MISCELLANEOUS ,0303 health sciences ,030219 obstetrics & reproductive medicine ,Hydrolysis ,[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,medicine.anatomical_structure ,Endocrinologie et métabolisme ,Receptors, FSH ,Female ,Gonadotropin ,Signal transduction ,Algorithms ,Signal Transduction ,endocrine system ,medicine.medical_specialty ,medicine.drug_class ,Biology ,Models, Biological ,reproduction ,03 medical and health sciences ,modèle mathématique ,femelle ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,Humans ,Cyclic adenosine monophosphate ,Ovarian follicle ,030304 developmental biology ,Endocrinology and metabolism ,ovaire ,adénosine monophosphate cyclique ,Models, Theoretical ,Endocrinology ,chemistry ,Follicle Stimulating Hormone ,Follicle-stimulating hormone receptor - Abstract
During the terminal part of their development, ovarian follicles become totally dependent on gonadotropin supply to pursue their growth and maturation. Both gonadotropins, follicle-stimulating hormone (FSH) and luteining hormone (LH), operate mainly through stimulatory G protein-coupled receptors, their signal being transduced by the activation of the enzyme adenylyl cyclase and the production of second-messenger cAMP. In this paper, we develop a mathematical model of the dynamics of the coupling between FSH receptor stimulation and cAMP synthesis. This model takes the form of a set of nonlinear, ordinary differential equations that describe the changes in the different states of FSH receptors (free, bound, phosphorylated, and internalized), coupling efficiency (activated adenylyl cyclase), and cAMP response. Classical analysis shows that, in the case of constant FSH signal input, the system converges to a unique, stable equilibrium state, whose properties are here investigated. The system also appears to be robust to nonconstant input. Particular attention is given to the influence of biologically relevant parameters on cAMP dynamics.
- Published
- 2001
37. Extracellular matrix regulates ovine granulosa cell survival, proliferation and steroidogenesis: relationships between cell shape and function
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C. Pisselet, C Huet, B Mandon-Pépin, Philippe Monget, Danielle Monniaux, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
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[SDV]Life Sciences [q-bio] ,Endocrinology, Diabetes and Metabolism ,Extracellular matrix ,0302 clinical medicine ,Endocrinology ,Laminin ,Insulin-Like Growth Factor I ,Cells, Cultured ,Progesterone ,ComputingMilieux_MISCELLANEOUS ,0303 health sciences ,030219 obstetrics & reproductive medicine ,Estradiol ,biology ,Progesterone secretion ,Extracellular Matrix ,[SDV] Life Sciences [q-bio] ,Female ,Collagen ,Cell Division ,hormones, hormone substitutes, and hormone antagonists ,Type I collagen ,endocrine system ,medicine.medical_specialty ,Cell Survival ,Granulosa cell ,[INFO] Computer Science [cs] ,03 medical and health sciences ,Internal medicine ,Cell Adhesion ,medicine ,Animals ,OSTRADIOL ,[INFO]Computer Science [cs] ,Polyhydroxyethyl Methacrylate ,Cell Size ,030304 developmental biology ,Analysis of Variance ,Granulosa Cells ,Sheep ,Heparin ,Follicular fluid ,Fibronectins ,Estradiol secretion ,Fibronectin ,biology.protein ,Follicle Stimulating Hormone ,Gels - Abstract
The extracellular matrix (ECM), constituting the follicular basal lamina and present also between follicular cells and in the follicular fluid, is believed to regulate granulosa cell (GC) function during follicular development. Ovine GCs isolated from small (1-3 mm in diameter) or large (4-7 mm in diameter) antral follicles were cultured on various pure ECM components (type I collagen, fibronectin, laminin), synthetic substrata enhancing (RGD peptides) or impairing (poly 2-hydroxyethylmethacrylate (poly-hema)) cell adhesion, or in the presence of heparin. The effects of these factors, used alone or in combination with IGF-I and/or FSH, were evaluated in terms of GC spread, survival, proliferation and steroidogenesis. When grown on type I collagen (CI) gel, poly-hema or heparin, GCs from both large and small follicles exhibited a round shape and a low proliferation rate. Compared with non-coated plastic substratum as a control, these ECM or synthetic compounds enhanced estradiol secretion and reduced progesterone secretion by large-follicle GCs. In contrast, GCs from both large and small follicles spread extensively on CI coating, fibronectin, laminin and RGD peptides. Fibronectin and laminin dramatically increased the proliferation rate and enhanced survival of GCs from both origins. Moreover, fibronectin, laminin and RGD peptides reduced estradiol secretion by large-follicle GCs. Unexpectedly, CI coating increased estradiol secretion and reduced progesterone secretion by large-follicle GCs, suggesting that type I collagen was able to maintain estradiol secretion independently of GC shape. Finally, GC responsiveness to IGF-I and FSH, in terms of proliferation and steroidogenesis, was generally maintained when cells were grown on ECM components, RGD peptides and in the presence of heparin. However, when large-follicle GCs were grown as non-adherent clusters (as observed on poly-hema) basal and IGF-I- and/or FSH-stimulated progesterone secretions were totally abolished. Overall, this study shows that GC shape, survival, proliferation and steroidogenesis can be modulated in vitro by pure ECM components in a specific and coordinated manner. It is suggested that, in vivo, fibronectin and laminin would sustain follicular development by enhancing the survival and proliferation of GCs, whereas type I collagen might participate in the maintenance of estradiol secretion in large antral follicles.
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- 2001
38. Chronology of Events Accompanying Follicular Atresia in Hypophysectomized Ewes. Changes in Levels of Steroidogenic Enzymes, Connexin 43, Insulin-Like Growth Factor II/Mannose 6 Phosphate Receptor, Extracellular Matrix Components, and Matrix Metalloproteinases1
- Author
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Philippe Monget, A. Locatelli, Christelle Hennequet, Danielle Monniaux, Claudine Pisselet, and Clotilde Huet
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endocrine system ,medicine.medical_specialty ,Hypophysectomy ,Granulosa cell ,medicine.medical_treatment ,Follicular Atresia ,Biology ,Receptor, IGF Type 2 ,Aromatase ,Estrus ,Internal medicine ,Follicular phase ,medicine ,Animals ,Ovarian follicle ,Extracellular Matrix Proteins ,Granulosa Cells ,Sheep ,Follicular atresia ,Theca interna ,Metalloendopeptidases ,Steroid 17-alpha-Hydroxylase ,Cell Biology ,General Medicine ,Luteinizing Hormone ,medicine.disease ,Fibronectins ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,Gelatinases ,Theca ,Connexin 43 ,Theca Cells ,Atresia ,Female ,Collagen - Abstract
The chronology of changes in levels of some proteins known to be altered during atresia of ovarian follicles was studied in ewes hypophysectomized at the end of the follicular phase of the estrous cycle. This study was performed by quantitative immunohistochemistry and zymography on large antral follicles (diameter > 3.5 mm) normally destined to ovulate, recovered 24, 36, or 72 h after pituitary ablation. The process of atresia was followed by comparing healthy follicles from intact ewes, with early atretic follicles recovered 24 h after hypophysectomy, clearly atretic follicles recovered 36 h after hypophysectomy, and late atretic follicles recovered 72 h after hypophysectomy. The earliest events of atresia induced by hypophysectomy were detected in theca and consisted of a strong decrease in levels of fibronectin (p < 0.001) and insulin-like growth factor II/mannose 6 phosphate (IGF-II/M6P) receptors (p < 0.05), occurring within the first 24 h following pituitary ablation. In intact animals, similar changes were observed in theca of early atretic follicles, suggesting that these changes may be important events involved in the onset of follicular atresia. In parallel, intrafollicular levels of matrix metalloproteinase-2 and -9 were shown to increase as early as 24 h after hypophysectomy, and a further increase was observed until 72 h after hypophysectomy. These early events were followed by the disappearance of P450 aromatase in granulosa cells 36 h after hypophysectomy (p < 0.05), and a progressive decrease in levels of P450 17alpha-hydroxylase in the theca interna and of gap junction protein connexin-43 in granulosa cells; these markers were still detectable in late atretic follicles 72 h after hypophysectomy. The increase in levels of fibronectin, type IV collagen, laminin, and IGF-II/M6P receptors within granulosa cell layers (p < 0.05) was significant only in late atretic stages, suggesting that these changes may be consequences rather than causes of atresia.
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- 1998
39. Growth kinetics of the granulosa cell population in ovarian follicles: an approach by mathematical modelling
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E. Jolivet, Danielle Monniaux, M. A. Gruet, F. Clément, M. Terqui, Philippe Monget, Unité de biométrie et intelligence artificielle de jouy, Institut National de la Recherche Agronomique (INRA), and Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly
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medicine.medical_specialty ,Cell division ,[SDV]Life Sciences [q-bio] ,Granulosa cell ,Population ,Cell ,Apoptosis ,Biology ,Models, Biological ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Follicular phase ,medicine ,Animals ,Humans ,Compartment (development) ,[INFO]Computer Science [cs] ,education ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,Original Paper ,0303 health sciences ,education.field_of_study ,Granulosa Cells ,Cell Differentiation ,Cell Biology ,General Medicine ,Cell cycle ,Cell biology ,Endocrinology ,medicine.anatomical_structure ,Female ,Cell Division ,030217 neurology & neurosurgery - Abstract
This paper describes, from a mathematical viewpoint, the cellular changes in the granulosa of ovarian follicles during their terminal development. A dynamic model takes into account the processes of (1) cell division, (2) exit from the cell cycle towards differentiation, and (3) apoptotic cell death. Proliferative cells leave the cycle in an irreversible way. The risk of entering apoptosis applies to non-cycling cells. Changes in the cell numbers and in the growth fraction are derived from differential equations. The transitions between the different cell states are ruled by time-dependent rates. Numerical applications of the model concern ovulating and degenerating ovarian follicles in the ewe. The main feature of the ovulating case is the progressive exhaustion of the proliferating compartment for the benefit of the non-cycling cells. From an initial mainly proliferative state the granulosa progressively switches to a highly differentiated state, so that the growth fraction continuously decreases. In the atretic cases, the pattern of changes in the total viable cell number is influenced by the follicular age at the onset of the apoptotic process and by the intensity of the cell death rate. As apoptosis affects the non-cycling cells, the growth fraction is no longer strictly decreasing. The sensitivity of the model to the parameters is studied in a more general framework than the granulosa cell population.
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- 1997
40. Coupled somatic cell kinetics and germ cell growth: multiscale model-based insight on ovarian follicular development
- Author
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Thomas Stiehl, Frédérique Clément, Philippe Michel, Danielle Monniaux, Institut Camille Jordan [Villeurbanne] (ICJ), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Interdisciplinary Center for Scientic Computing (IWR). Im Neuenheimer Feld 368. 69120 Hei- delberg - Germany., Interdisciplinary Center for Scientic Computing, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Multiscale dYnamiCs in neuroENdocrine AxEs (Mycenae), Inria de Paris, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut Camille Jordan (ICJ), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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Oocyte ,stochastic individual based models ,Somatic cell ,medicine.medical_treatment ,[SDV]Life Sciences [q-bio] ,Population ,General Physics and Astronomy ,Reproductive technology ,Biology ,03 medical and health sciences ,0302 clinical medicine ,granulosa ,Follicular phase ,medicine ,Ovarian follicle ,[MATH]Mathematics [math] ,education ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,030219 obstetrics & reproductive medicine ,In vitro fertilisation ,Ecological Modeling ,General Chemistry ,3. Good health ,Computer Science Applications ,Cell biology ,medicine.anatomical_structure ,ovarian follicle ,cell proliferation ,Modeling and Simulation ,Germ cell - Abstract
International audience; The development of ovarian follicles is a unique instance of a morphogenesis process still occurring during adult life and resulting from the interactions between somatic and germ cells. In mammals, the initiation of follicular development from the pool of resting follicles is characterized by an increase in the oocyte size concomitant with the surrounding somatic cells proliferating to build an avascular tissue called granulosa. We present a stochastic individual-based model describing the first stages of follicular development, where the cell population is structured with respect to age (progression within the cell cycle) and space (radial distance from the oocyte). The model accounts for the molecular dialogue existing between the oocyte and granulosa cells. Three dynamically interacting scales are considered in the model: (i) a microscopic, local scale corresponding to an individual cell embedded in its immediate environment, (ii) a mesoscopic, semi-local scale corresponding to anatomical or functional areas of follicles and (iii) a macroscopic, global scale corresponding to the morphology of the follicle. Numerical simulations are performed to reproduce the 3D morphogenesis of follicles and follow simultaneously the detailed spatial distribution of individual granulosa cells, their organization as concentric layers or functional cell clones and the increase in the follicle size. Detailed quantitative simulation results are provided in the ovine species, in which well characterized genetic mutations lead to a variety of phenotypic follicle morphogenesis. The model can help to explain pathological situations of imbalance between oocyte growth and follicular cell proliferation 1. Introduction. A highly efficient reproductive capacity is a major advantage for species preservation, faced with the natural selection process, and for individuals within species. In mammalian females, ovarian function is the subject of intensive investigations with the aim to improve the reproductive capacity of domestic and wild animal species and to treat ovarian failures leading to infertility in humans. The issues are crucial for both clinical and zootechnical applications. In humans, the prevalence of the polycystic ovarian syndrome, which is a main cause of infertility, has been estimated at up to 10% among reproductive-age women [19]. Improvement of reproductive biotechnologies, including in vitro fertilization, intra-cytoplasmic sperm injection, frozen embryo replacements and egg donation, is a key issue for a better management of reproduction. Improving the knowledge upon ovarian function and its control will help to improve the success of assisted reproductive technologies, hence to prevent ovarian failure or hyperstimulation syndrome in women and to manage
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- 2013
41. Postnatal Leptin Promotes Organ Maturation and Development in IUGR Piglets
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Daphné Brisard, Thibaut Larcher, Claude-Narcisse Niamba, Danielle Monniaux, Arieh Gertler, Samir Boukthir, Latifa Abdennebi-Najar, Paul Guilloteau, Michal Mickiewicz, Jean Djiane, and Linda Attig
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Literature ,Multidisciplinary ,business.industry ,media_common.quotation_subject ,Science ,lcsh:R ,lcsh:Medicine ,Correction ,Legend ,Medicine ,lcsh:Q ,lcsh:Science ,business ,media_common - Abstract
The titles and legends for Figures 2 through 5 were incorrectly switched. The title and legend currently appearing with Figure 2 belong with Figure 3, the title and legend currently appearing with Figure 3 belong with Figure 4, the title and legend currently appearing with Figure 4 belong with Figure 5, and the title and legend currently appearing with Figure 5 belong with Figure 2. The Figures themselves are in correct order.
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- 2013
42. Postnatal leptin promotes organ maturation and development in IUGR piglets
- Author
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Thibaut Larcher, Claude-Narcisse Niamba, Daphné Brisard, Paul Guilloteau, Danielle Monniaux, Michal Mickiewicz, Arieh Gertler, Samir Boukthir, Latifa Abdennebi-Najar, Linda Attig, Jean Djiane, Institut Polytechnique La Salle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Développement et Pathologie du Tissu Musculaire (DPTM), Ecole Nationale Vétérinaire de Nantes-Institut National de la Recherche Agronomique (INRA), Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Alimentation Adaptions Digestives, Nerveuses et Comportementales (ADNC), Institut National de la Recherche Agronomique (INRA), The Robert Smith Faculty of Agriculture, Food and Environment, Neurobiologie de l'Olfaction et de la Prise Alimentaire (NOPA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Alimentation Adaptations Digestives, Nerveuse et Comportementales (ADNC), Neurobiologie de l'Olfaction et Modélisation en Imagerie (NOeMI), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Leptin ,Male ,Anatomy and Physiology ,Swine ,Organogenesis ,Lymphocyte ,animal diseases ,lcsh:Medicine ,Pediatrics ,Endocrinology ,0302 clinical medicine ,Molecular Cell Biology ,Biologie de la reproduction ,Morphogenesis ,Growth Retardation ,lcsh:Science ,RCIU ,Reproductive Biology ,0303 health sciences ,Fetal Growth Retardation ,Multidisciplinary ,Gene Expression Regulation, Developmental ,Forkhead Transcription Factors ,Animal Models ,Organ Size ,medicine.anatomical_structure ,Liver ,Alimentation et Nutrition ,leptine ,Medicine ,Female ,Pancreas ,CD79 Antigens ,Research Article ,medicine.medical_specialty ,Histology ,030209 endocrinology & metabolism ,Spleen ,Biology ,Injections, Intramuscular ,03 medical and health sciences ,Follicle ,Model Organisms ,Fetus ,Internal medicine ,Parenchyma ,medicine ,Food and Nutrition ,Animals ,Humans ,Gonads ,030304 developmental biology ,Growth Control ,lcsh:R ,Infant, Newborn ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Small intestine ,Animals, Newborn ,Immune System ,lcsh:Q ,Neonatology ,Organism Development ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Developmental Biology - Abstract
Babies with intra-uterine growth restriction (IUGR) are at increased risk for experiencing negative neonatal outcomes due to their general developmental delay. The present study aimed to investigate the effects of a short postnatal leptin supply on the growth, structure, and functionality of several organs at weaning. IUGR piglets were injected from day 0 to day 5 with either 0.5 mg/kg/d leptin (IUGRLep) or saline (IUGRSal) and euthanized at day 21. Their organs were collected, weighed, and sampled for histological, biochemical, and immunohistochemical analyses. Leptin induced an increase in body weight and the relative weights of the liver, spleen, pancreas, kidneys, and small intestine without any changes in triglycerides, glucose and cholesterol levels. Notable structural and functional changes occurred in the ovaries, pancreas, and secondary lymphoid organs. The ovaries of IUGRLep piglets contained less oogonia but more oocytes enclosed in primordial and growing follicles than the ovaries of IUGRSal piglets, and FOXO3A staining grade was higher in the germ cells of IUGRLep piglets. Within the exocrine parenchyma of the pancreas, IUGRLep piglets presented a high rate of apoptotic cells associated with a higher trypsin activity. In the spleen and the Peyer's patches, B lymphocyte follicles were much larger in IUGRLep piglets than in IUGRSal piglets. Moreover, IUGRLep piglets showed numerous CD79(+) cells in well-differentiated follicle structures, suggesting a more mature immune system. This study highlights a new role for leptin in general developmental processes and may provide new insight into IUGR pathology.
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- 2013
43. The Highly Prolific Phenotype of Lacaune Sheep Is Associated with an Ectopic Expression of the B4GALNT2 Gene within the Ovary
- Author
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Florent Woloszyn, Julien Sarry, Catherine Viguié, Jérôme Lluch, Stéphane Fabre, Laurence Drouilhet, Danielle Monniaux, Philippe Mulsant, Kamila Canale Tabet, Loys Bodin, Camille Mansanet, Philippe Bardou, Grégoire Harichaux, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Génome et Transcriptome - Plateforme Génomique (GeT-PlaGe), Institut National de la Recherche Agronomique (INRA)-Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Toxicologie Alimentaire (UTA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Ecole Nationale Vétérinaire de Toulouse (ENVT), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire ( LGC ), Institut National de la Recherche Agronomique ( INRA ) -Ecole Nationale Vétérinaire de Toulouse, Physiologie de la reproduction et des comportements [Nouzilly] ( PRC ), Institut National de la Recherche Agronomique ( INRA ) -Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique ( CNRS ), Toxicologie Alimentaire ( UTA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Station d'Amélioration Génétique des Animaux ( SAGA ), Institut National de la Recherche Agronomique ( INRA ), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Ovulation ,Cancer Research ,Glycosylation ,lcsh:QH426-470 ,Granulosa cell ,[SDV]Life Sciences [q-bio] ,Locus (genetics) ,Biology ,03 medical and health sciences ,Ovarian Follicle ,Gene expression ,Genetics ,Animals ,Humans ,RNA, Messenger ,Allele ,Molecular Biology ,Gene ,Genetics (clinical) ,Ecology, Evolution, Behavior and Systematics ,Sheep, Domestic ,030304 developmental biology ,2. Zero hunger ,Regulation of gene expression ,0303 health sciences ,Granulosa Cells ,[ SDV ] Life Sciences [q-bio] ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Luteinizing Hormone ,040201 dairy & animal science ,Major gene ,Molecular biology ,lcsh:Genetics ,Fertility ,Gene Expression Regulation ,N-Acetylgalactosaminyltransferases ,Ectopic expression ,Female ,Infertility, Female ,Research Article - Abstract
Prolific sheep have proven to be a valuable model to identify genes and mutations implicated in female fertility. In the Lacaune sheep breed, large variation in litter size is genetically determined by the segregation of a fecundity major gene influencing ovulation rate, named FecL and its prolific allele FecLL. Our previous work localized FecL on sheep chromosome 11 within a locus of 1.1 Mb encompassing 20 genes. With the aim to identify the FecL gene, we developed a high throughput sequencing strategy of long-range PCR fragments spanning the locus of FecLL carrier and non-carrier ewes. Resulting informative markers defined a new 194.6 kb minimal interval. The reduced FecL locus contained only two genes, insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) and beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), and we identified two SNP in complete linkage disequilibrium with FecLL. B4GALNT2 appeared as the best positional and expressional candidate for FecL, since it showed an ectopic expression in the ovarian follicles of FecLL/FecLL ewes at mRNA and protein levels. In FecLL carrier ewes only, B4GALNT2 transferase activity was localized in granulosa cells and specifically glycosylated proteins were detected in granulosa cell extracts and follicular fluids. The identification of these glycoproteins by mass spectrometry revealed at least 10 proteins, including inhibin alpha and betaA subunits, as potential targets of B4GALNT2 activity. Specific ovarian protein glycosylation by B4GALNT2 is proposed as a new mechanism of ovulation rate regulation in sheep, and could contribute to open new fields of investigation to understand female infertility pathogenesis., Author Summary Prolific sheep have proven to be a valuable model to identify genes and mutations implicated in ovarian function and female fertility. Indeed, fecundity genes of the Bone Morphogenetic Protein (BMP) family discovered in sheep was evidenced as genetic candidates to explain female infertility pathologies. Studying French Lacaune sheep breed, we discovered another fecundity gene named B4GALNT2, encoding a glycosylation enzyme that is not related to the BMP family. The high prolificacy of Lacaune sheep was explained by overexpression of B4GALNT2 in the ovary leading to atypical glycosylation of inhibin, an important hormone regulating ovarian function. Our findings open promising fields of investigation to better understand female fertility disorders.
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- 2013
44. Expression of Messenger Ribonucleic Acids of Insulin-Like Growth Factor Binding Proteins-2, -4, and -5 in the Ovine Ovary: Localization and Changes during Growth and Atresia of Antral Follicles1
- Author
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Claudine Pisselet, A. Locatelli, François Gasser, Francis Benne, Nathalie Besnard, Philippe Monget, Danielle Monniaux, and François Hatey
- Subjects
endocrine system ,Messenger RNA ,medicine.medical_specialty ,Ovary ,Cell Biology ,General Medicine ,In situ hybridization ,Biology ,medicine.disease ,medicine.anatomical_structure ,Endocrinology ,Reproductive Medicine ,Theca ,Atresia ,Internal medicine ,Follicular phase ,Gene expression ,medicine ,Ovarian follicle ,hormones, hormone substitutes, and hormone antagonists - Abstract
In the sheep as in many mammalian species, growth and atresia of antral follicles are characterized, respectively, by a decrease and a high increase in the intrafollicular levels of insulin-like growth factor binding proteins of less than 40 kDa (IGFBPs < 40 kDa), mainly IGFBP-2, -4, and -5. The objective of this study was to investigate whether such changes are associated with changes in follicular expression of the corresponding mRNA. For this purpose, ovaries were recovered from ewes slaughtered at the end of follicular phase (i.e., 30 h after progestagen sponge removal; control ewes) or at 24 h, 36 h or 72 h after hypophysectomy (hypox) performed 30 h after sponge removal. The expression of mRNA of IGFBPs of less than 40 kDa (IGFBPs < 40 kDa mRNA) was studied in ovine antral follicles from control and hypox ewes by in situ hybridization using [35S]-labeled human IGFBP-2, -4, and -5 cRNA as probes. In control ewes, IGFBP-2 mRNA was mainly expressed in granulosa as a gradient in healthy follicles, the expression being higher in granulosa cells close to the basal membrane than in granulosa cells bordering the antrum and within the cumulus. The level of IGFBP-2 mRNA was lower both in granulosa cells close to the basal membrane and in those bordering the antrum from small follicles than in the corresponding compartments of granulosa cells from large healthy follicles (p < 0.05). In healthy follicles, IGFBP-4 and -5 mRNA were mainly expressed in thecal cells. No change in level of IGFBP-4 mRNA was observed between small and large follicles, whereas the level of IGFBP-5 mRNA tended to be lower in thecal cells from large compared to small follicles (p = 0.055). In atretic follicles, expression of IGFBPs < 40 kDa mRNA strongly increased in granulosa (IGFBP-2 and -5, p < 0.01) and in thecal cells (IGFBP-2 and -4, p < 0.01). In hypox ewes, the chronology of changes in expression of follicular IGFBPs < 40 kDa mRNA and in intrafollicular levels of the corresponding proteins was studied during atresia of large antral follicles. Early atresia of large follicles was associated with a strong decrease in intrafollicular estradiol levels (p < 0.001); an increase in intrafollicular levels of IGFBP-2, -4, and -5 (p < 0.001) an increase in both IGFBP-2 (p < 0.001) and -5 (p < 0.01) mRNA expression in granulosa and thecal cells; but no changed in IGFBP-4 mRNA expression. Late atresia of large follicles was associated with a further decrease in intrafollicular estradiol levels (p < 0.01); a further increase in intrafollicular levels of IGFBP-2, -4, and -5 (p < 0.001); an increase in IGFBP-4 (p < 0.01) and -5 (p < 0.05) mRNA expression in theca and granulosa, respectively; a decrease in IGFBP-5 mRNA expression in theca (p < 0.05); but no further increase in IGFBP-2 mRNA expression. Overall, these data suggest that the decrease and the increase in expression of mRNA of follicular IGFBPs < 40 kDa during follicular growth and atresia, respectively, are involved in the decrease and the increase in intrafollicular levels of the corresponding proteins. Moreover, the increases in expression of follicular IGFBPs < 40 kDa during atresia of large follicles in hypophysectomized ewes followed a specific time course, the increase in IGFBP-2 and -5 mRNA expression being early than the increase in IGFBP-4 mRNA expression.
- Published
- 1996
45. Anti-Müllerian hormone plasma concentration in prepubertal ewe lambs as a predictor of their fertility at a young age
- Author
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Danielle Monniaux, J.L. Alabart, Pascal Mermillod, Bernard Lahoz, J. Folch, Unidad de Tecnologia en produccion Animal, Centro de Investigacion y Tecnologia Agroalimentaria de Aragon (CITA), Unidad de Tecnologia en Produccion Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Centro de Investigacion y Technologia Agroalimentaria de Aragon (CITA), INIA (Ministerio de Ciencia e Innovacion, Spain) RTA-2011-128 RZP-2010-002, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Alabart, José L., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Anti-Mullerian Hormone ,prepuberte ,reproduction animale ,Chorionic Gonadotropin ,Ovinos ,0302 clinical medicine ,ovin ,Follicular phase ,Sexual Maturation ,Mating ,media_common ,2. Zero hunger ,030219 obstetrics & reproductive medicine ,lcsh:Veterinary medicine ,analyse statistique ,biology ,agneau ,brebis ,Anti-Müllerian hormone ,04 agricultural and veterinary sciences ,General Medicine ,hormone antimullérienne ,Female ,Autre (Sciences du Vivant) ,Research Article ,Ovulation ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,endocrine system ,media_common.quotation_subject ,Fertility ,Andrology ,03 medical and health sciences ,Predictive Value of Tests ,Animals ,Endocrine system ,Equine chorionic gonadotropin ,plasma ,Sheep ,General Veterinary ,ovaire ,fertilité animale ,0402 animal and dairy science ,Hormonas ,veterinary(all) ,040201 dairy & animal science ,Young age ,Fertilidad ,Producción y sanidad animal ,biology.protein ,lcsh:SF600-1100 ,Biomarkers ,Hormone - Abstract
Background In mammals, the ovarian follicular reserve is highly variable between individuals and impacts strongly on ovarian function and fertility. Nowadays, the best endocrine marker of this reserve in human, mouse and cattle is the anti-Müllerian hormone (AMH). The objectives of this work were to determine whether AMH could be detected in the plasma of prepubertal ewe lambs and to assess its relationship with their fertility at a young age. Results Plasma was taken from 76 Rasa Aragonesa ewe lambs at 3.6 months of age for AMH determination. Simultaneously, 600 IU equine chorionic gonadotropin (eCG) was administered and the number of ovulations recorded 6 days later. AMH was detected in 93% of the lambs, and the concentrations were about 3–4-fold higher in ovulating than in non-ovulating lambs (P P P Conclusions Plasma AMH concentration might be a reliable marker of the ovarian status of prepubertal ewe lambs, reflecting their ability to respond to eCG stimulation. A single AMH measurement performed on ewe lambs early in age could be useful to select for replacement ewes with a higher predicted fertility at first mating.
- Published
- 2012
46. Anti-Müllerian hormone as a predictive endocrine marker for embryo production in the goat
- Author
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Danielle Monniaux, Stéphane Fabre, Juliette Cognie, Natividad Poulin, G. Baril, Anne-Lyse Lainé, Peggy Jarrier, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), This work was supported by specific funding (Incitement Grant to sustain innovation and research valorization) from the INRA PHASE division., Monniaux, Danielle, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Anti-Mullerian Hormone ,Embryology ,cellule de granulosa ,hormone animale ,reproduction animale ,follicule ovarien ,0302 clinical medicine ,Endocrinology ,Ovarian Follicle ,Seasonal breeder ,Biologie de la reproduction ,030219 obstetrics & reproductive medicine ,biology ,Goats ,goat ,reproductive biology ,Obstetrics and Gynecology ,Anti-Müllerian hormone ,Embryo ,in vitro ,04 agricultural and veterinary sciences ,Female ,Seasons ,embryo production ,hormones, hormone substitutes, and hormone antagonists ,endocrine marker ,medicine.medical_specialty ,endocrine system ,chèvre ,Granulosa cell ,production d'embryon ,Embryonic Development ,03 medical and health sciences ,Follicle ,developmental biology ,Ovulation Induction ,Internal medicine ,Reproductive biology ,medicine ,Animals ,RNA, Messenger ,caprin ,Estrous cycle ,urogenital system ,hormone anti müllérienne ,0402 animal and dairy science ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Cell Biology ,Embryo, Mammalian ,040201 dairy & animal science ,Reproductive Medicine ,embryon ,biology.protein ,Follicle Stimulating Hormone ,Biomarkers ,Hormone - Abstract
Recently, we demonstrated the relationship between anti-Müllerian hormone (AMH) circulating concentrations, ovarian follicles, and embryo production in cattle. However, they have not yet been established in a species with a seasonal breeding activity. Thus, goats were subjected to repeated in vivo embryo production during the breeding season, at the end of the breeding season, and at the end of the anestrus season. Embryo production after FSH treatment was highly repeatable for each goat. Plasma AMH concentrations, measured before the first FSH treatment, were highly correlated with the number of collected, transferable, and freezable embryos, resulting from the three sessions of embryo production. Plasma AMH concentrations transiently decreased after each exogenous FSH treatment, but they showed little change with season, and no relationship was observed between AMH and endogenous FSH concentrations during seasonal transitions. Follicles of 1–5 mm in diameter were the main target of the FSH treatment and were major contributors to circulating AMH concentrations. Granulosa cell AMH expression decreased as the follicle approached terminal development, while the expression of maturation markers (CYP19A1 and FSHR) increased. In conclusion, circulating AMH concentrations can be predictive of the capacity of a donor goat to produce high or low numbers of high-quality embryos. This prediction could be accurately made from a single blood measurement of AMH during either breeding or anestrus seasons. Variability in the number of gonadotropin-responsive follicles of 1–5 mm in diameter between individuals resulted in the differences in circulating AMH concentrations measured between individuals.
- Published
- 2011
47. Anti-mullerian hormone is an endocrine marker of ovarian gonadotropin-responsive follicles and can help to predict superovulatory responses in the cow
- Author
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Benoit Remy, Claire Médigue, Frédérique Clément, Eric Briant, Jean-Luc Touzé, Stéphane Fabre, Charlène Rico, Nathalie di Clemente, Mickaël Dupont, Danielle Monniaux, Martine Bontoux, Jean-François Beckers, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), SIgnals and SYstems in PHysiology & Engineering (SISYPHE), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Endocrinologie et Génétique de la Reproduction et du Développement, Université Paris-Sud - Paris 11 (UP11)-IFR13-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (Unité Expérimentale de Physiologie Animale de l‘Orfrasiére - UE PAO), Institut National de la Recherche Agronomique (INRA), Laboratory of Endocrinology and Animal Reproduction - Laboratoire d'Endocrionologie et de Physiologie de la Reproduction Animale (Laboratory of Endocrinology and Animal Reproduction - Laboratoire d'Endocrionologie et de Physiologie de la Reproduction Animale), Université de Liège, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Anti-Mullerian Hormone ,medicine.medical_specialty ,endocrine system ,endocrine system diseases ,medicine.drug_class ,Population ,Superovulation ,Ovary ,Biology ,03 medical and health sciences ,Aromatase ,0302 clinical medicine ,Ovarian Follicle ,Predictive Value of Tests ,Internal medicine ,Follicular phase ,medicine ,Animals ,Endocrine system ,RNA, Messenger ,Ovarian follicle ,education ,Progesterone ,Ultrasonography ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,education.field_of_study ,Granulosa Cells ,030219 obstetrics & reproductive medicine ,Estradiol ,Reverse Transcriptase Polymerase Chain Reaction ,Anti-Müllerian hormone ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Cell Biology ,General Medicine ,Antral follicle ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,biology.protein ,Cattle ,Female ,Gonadotropin ,hormones, hormone substitutes, and hormone antagonists - Abstract
International audience; The major limitation to the development of embryo production in cattle is the strong between-animal variability in ovulatory response to FSH-induced superovulation, mainly due to differences in ovarian activity at the time of treatment. This study aimed to establish whether anti-Müllerian hormone (AMH) was an endocrine marker of follicular populations in the cow, as in human, and a possible predictor of the ovarian response to superovulation. Anti-Müllerian hormone concentrations in plasma varied 10-fold between cows before treatment and were found to be highly correlated with the numbers of 3- to 7-mm antral follicles detected by ovarian ultrasonography before treatment (r=0.79, P
- Published
- 2009
48. Intrafollicular Steroids and Anti-Müllerian Hormone During Normal and Cystic Ovarian Follicular Development in the Cow1
- Author
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Stéphane Fabre, Martine Bontoux, Jean-Luc Touzé, Corinne Belville, Jean-Yves Picard, Danielle Monniaux, Charlène Rico, Nathalie di Clemente, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Endocrinologie et Génétique de la Reproduction et du Développement, Université Paris-Sud - Paris 11 (UP11)-IFR13-Institut National de la Santé et de la Recherche Médicale (INSERM), Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'embryologie cellulaire et moléculaire (LECM), Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Collège de France (CdF), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), and Université de Toulouse (UT)-Université de Toulouse (UT)
- Subjects
medicine.medical_specialty ,endocrine system ,anti mullerian hormone ,follicular development ,Ovary ,follicle ,[SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Follicular phase ,parasitic diseases ,medicine ,Cyst ,Testosterone ,030304 developmental biology ,0303 health sciences ,030219 obstetrics & reproductive medicine ,biology ,Follicular Cyst ,Theca interna ,Anti-Müllerian hormone ,Cell Biology ,General Medicine ,Antral follicle ,medicine.disease ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,steroid hormone ,biology.protein ,ovary - Abstract
International audience; Development of follicular cysts is a frequent ovarian dysfunction in cattle. Functional changes that precede cyst formation are unknown, but a role for anti-Müllerian hormone (AMH) in the development of follicular cysts has been suggested in humans. This study aimed to characterize intrafollicular steroids and AMH during follicular growth in a strain of beef cows exhibiting a high incidence of occurrence of follicular cysts. Normal follicular growth and cyst development were assessed by ovarian ultrasonography scanning during the 8 days before slaughtering. Experimental regression of cysts was followed by rapid growth of follicles that reached the size of cysts within 3-5 days. These young cysts exhibited higher intrafollicular concentrations of testosterone, estradiol-17beta, and progesterone than large early dominant follicles did in normal ovaries, but they exhibited similar concentrations of AMH. Later-stage cysts were characterized by hypertrophy of theca interna cells, high intrafollicular progesterone concentration, and high steroidogenic acute regulatory protein mRNA expression in granulosa cells. Progesterone and AMH concentrations in the largest follicles (> or =10 mm) and cysts were negatively correlated (r = -0.45, P < 0.01). Smaller follicles (
- Published
- 2008
49. Intrafollicular steroids and anti-mullerian hormone during normal and cystic ovarian follicular development in the cow
- Author
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Danielle, Monniaux, Nathalie di, Clemente, Jean-Luc, Touzé, Corinne, Belville, Charlène, Rico, Martine, Bontoux, Jean-Yves, Picard, and Stéphane, Fabre
- Subjects
Anti-Mullerian Hormone ,Organ Size ,Phosphoproteins ,Follicular Fluid ,Luteinization ,Ovarian Cysts ,Aromatase ,Ovarian Follicle ,Animals ,Cattle ,Female ,Testosterone ,Cholesterol Side-Chain Cleavage Enzyme ,RNA, Messenger ,Gonadal Steroid Hormones ,Progesterone - Abstract
Development of follicular cysts is a frequent ovarian dysfunction in cattle. Functional changes that precede cyst formation are unknown, but a role for anti-Müllerian hormone (AMH) in the development of follicular cysts has been suggested in humans. This study aimed to characterize intrafollicular steroids and AMH during follicular growth in a strain of beef cows exhibiting a high incidence of occurrence of follicular cysts. Normal follicular growth and cyst development were assessed by ovarian ultrasonography scanning during the 8 days before slaughtering. Experimental regression of cysts was followed by rapid growth of follicles that reached the size of cysts within 3-5 days. These young cysts exhibited higher intrafollicular concentrations of testosterone, estradiol-17beta, and progesterone than large early dominant follicles did in normal ovaries, but they exhibited similar concentrations of AMH. Later-stage cysts were characterized by hypertrophy of theca interna cells, high intrafollicular progesterone concentration, and high steroidogenic acute regulatory protein mRNA expression in granulosa cells. Progesterone and AMH concentrations in the largest follicles (or =10 mm) and cysts were negatively correlated (r = -0.45, P0.01). Smaller follicles (10 mm) exhibited higher intrafollicular testosterone and estradiol-17beta concentrations in ovaries with cysts compared to normal ovaries. During follicular growth, AMH concentration dropped in follicles larger than 5 mm in diameter and in a similar way in ovaries with and without cysts. In conclusion, enhanced growth and steroidogenesis in antral follicles10 mm preceded cyst formation in cow ovaries. Intrafollicular AMH was not a marker of cystic development in the cow, but low AMH concentrations in cysts were associated with luteinization.
- Published
- 2008
50. Does FSH signaling have a gender ?
- Author
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Elodie Kara, Pascale Crépieux, Danielle Monniaux, Florian Guillou, Charlotte Lécureuil, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Crépieux, Pascale
- Subjects
0303 health sciences ,endocrine system ,[SDV]Life Sciences [q-bio] ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,General Medicine ,Biology ,[INFO] Computer Science [cs] ,Molecular biology ,General Biochemistry, Genetics and Molecular Biology ,[SDV] Life Sciences [q-bio] ,03 medical and health sciences ,Follicle-stimulating hormone ,0302 clinical medicine ,030220 oncology & carcinogenesis ,FSH ,[INFO]Computer Science [cs] ,HORMONE GONADOTROPE ,[SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology ,hormones, hormone substitutes, and hormone antagonists ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology - Abstract
FSH is the main endocrine control of mamalian reproduction. FSH triggers somatic cells of the gonads which support germ cells metabolically, i.e. Sertoli cells of the seminiferous tubules, and granulosa cells harboring the oocyte, within the ovarian follicle. FSH leads to similar biological responses in both cell types since it stimulates proliferation and differentiation, according to the developmental stage. However, FSH receptor knock-out female mice are infertile, unlike male mice. Hence, FSH is not equally important in both sexes. Nevertheless, does FSH induce distinct signalling mechanisms in its target cells ? Here, we compare the signalling mechanisms induced by FSH in ovarian and testicular physiology., La FSH représente le principal contrôle endocrinien de la reproduction des Mammifères. Cette hormone cible les cellules somatiques des gonades qui assurent les apports nutritifs des cellules germinales : les cellules de Sertoli du tube séminifère et les cellules de la granulosa entourant l’ovocyte, au sein du follicule ovarien. La FSH induit des réponses biologiques analogues dans les deux types de cellules, puisque, en fonction du stade de développement, elle stimule leur prolifération ou leur différenciation. Pourtant, l’invalidation du récepteur de la FSH chez la souris aboutit à une stérilité totale chez la femelle, mais pas chez le mâle. La FSH n’a donc pas la même importance dans les deux sexes. Pour autant, induit-elle des mécanismes de signalisation distincts dans ses cellules cibles ? L’objectif de cette synthèse est de comparer les mécanismes de signalisation induits par la FSH dans la physiologie ovarienne et testiculaire.
- Published
- 2007
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