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Water scarcity in arid and semiarid areas highlights the importance of developing water-saving irrigation techniques. Soil extracellular enzyme activities (EEAs) and microbes are important for soil biochemical cycles and plant growth. However, little is understood about how soil EEAs, and microbial communities respond to water-saving irrigation systems and levels. Therefore, a field experiment was carried out over four consecutive wheat-maize seasons to explore the effects of surface irrigation (SI) and drip irrigation (DI) coupled with different irrigation levels (i.e. 65, 50, 35 and 20 mm/event, represents sufficient, mild deficit, moderate deficit and serious deficit irrigation, respectively) on the plant biomass, soil properties, soil EEAs, and bacterial community. The results revealed that mild deficit irrigation showed no significant impact on plant biomass, serious and moderate deficit irrigation significantly decreased crop biomass by 49.0 % and 26.6 %, respectively, compared with sufficient irrigation. However, the crop biomass of serious and moderate deficit irrigation under DI were 15.4 % and 17.4 % higher, respectively, than that under SI. Serious and moderate deficit irrigation significantly increased soil available K accumulation and soil urease activity, decreased bacterial richness, and shifted the bacterial community structure. No significant differences were found between mild deficit and sufficient irrigation. The relative abundances of Firmicutes, Gemmatimonadota, and Myxococcota significantly increased under serious deficit irrigation, while Acidobacteriota significantly decreased. Moderate deficit irrigation under DI did not shift bacterial community structure, while under SI did. Soil pH, electrical conductivity (EC), available K, and plant biomass were the major factors influencing the bacterial community compositions. Serious deficit irrigation reduced the complexity and stability of the soil bacterial co-occurrence network. The network of DI had more significant interactions among bacteria than the SI. Therefore, DI coupled with moderate deficit irrigation is more beneficial to maintain crop yield and the stability of bacterial community structure than SI.

Over-input of nitrogen (N) fertilizer coupled with flood irrigation resulted in low N and water productivity and serious NO3--N leaching in maize fields in the North China Plain (NCP). Drip-fertigation can enhance water-N use efficiency by precisely regulating water and N fertilizer application. In this study, a three-year drip-fertigation experiment on maize was carried out during the 2018–2020 growing seasons with three irrigation levels, i.e. 50 (W1, 100 %), 40 (W2, 80 %), and 30 mm (W3, 60 %), and five N levels, i.e. 0 (N0), 90 (N1), 180 (N2), 270 (N3), and 360 (N4) kg ha−1 were designed to investigate the responses of soil water consumption, NO3--N distribution, grain yield (GY), N uptake (UN), and water-N productivity to different irrigation and N application regimes. The results demonstrated that 80 % deficit irrigation (W2) obtained comparable GY to sufficient irrigation (W1), but significantly increased water productivity (WP) by 7.2 % compared with W1. Soil NO3--N contents in the 0–100 cm soil layers increased as the N application rate increased, but decreased with the increase of irrigation level. When the N application rate exceeded 180 kg N ha−1, soil NO3--N contents in the 60–100 cm soil layers were greatly increased. With the increasing N application rate, GY increased first and then tended to be stabilized when the N application rate reached 180 kg ha−1, UN showed an increased trend, while N agronomic efficiency (AEN) showed a decreased trend, the N recovery efficiency (REN) increased firstly and then decreased. When water consumption ranged from 374 to 388 mm and N application rate from 186 to 257 kg ha−1, respectively, GY and WP obtained 95 maximum values and AEN got 70 % maximum value, simultaneously. In conclusion, the regime of N application rate at about 180 kg ha−1 coupled with 40 mm irrigation level (per event) is recommended for drip-fertigated maize fields in the NCP.

IntroductionExtreme weather has occurred more frequently in recent decades, which results in more frequent drought disasters in the maize growing season. Severe drought often decreases remarkably plant growth and yield of maize, and even reduces significantly the quality of maize production, especially for waxy maize.ResultsTo study the changes in plant growth, fresh ear yield, and fresh grain quality of waxy maize under water deficits occurring at different growth stages, and further strengthen the field water management of waxy maize, water deficit experiments were carried out under a rain shelter in 2019 and 2020. Water deficit treatments were imposed respectively at the V6–VT (DV6–VT), VT–R2 (DVT–R2), and R2–R3 (DR2–R3) stages of waxy maize, and treatment with non-water deficit in the whole growing season was taken as the control (CK). The lower limit of soil water content was 50% of field capacity for a water deficit period and 65% of field capacity for a non-water deficit period.ResultsIn this study, water deficits imposed at V6–VT and VT–R2 stages decreased plant growth rate and leaf gas exchange parameters, accelerated leaf senescence, and limited ear growth of waxy maize, which resulted in 11.6% and 23.1% decreases in grains per ear, 19.4% and 7.3% declines in 100-grain weight, 20.3% and 14.2% losses in fresh ear yield in 2019 and 2020 growing seasons, respectively, while water deficit at R2–R3 stage had no significant effect on ear traits and fresh ear yield, but the fresh ear yield with husk of DR2–R3 decreased by 9.1% (P

Drought is one of the major environmental stresses, resulting in serious yield reductions in wheat production. Silicon (Si) has been considered beneficial to enhancing wheat resistance to drought stress. However, few studies have explored the mediated effects of foliar supplementation of Si on drought stress imposed at different wheat growth stages. Therefore, a field experiment was carried out to investigate the effects of Si supplementation on the physiological and biochemical responses of wheat to drought stress imposed at the jointing (D-jointing), anthesis (D-anthesis) and filling (D-filling) stages. Our results showed that a moderate water deficit markedly decreased the dry matter accumulation, leaf relative water content (LRWC), photosynthetic rate (Pn), stomatal conductance (Sc), transpiration rate (Tr) and antioxidant activity [peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT)]. On the contrary, it remarkably increased the content of osmolytes (proline, soluble sugar, soluble protein) and lipid peroxidation. The grain yields of D-jointing, D-anthesis and D-filling treatments were 9.59%, 13.9% and 18.9% lower, respectively, compared to the control treatment (CK). However, foliar supplementation of Si at the anthesis and filling stages significantly improved plant growth under drought stress due to the increased Si content. Consequently, the improvement in antioxidant activity and soluble sugar, and the reduction in the content of ROS, increased the LRWC, chlorophyll content, Pn, Sc and Tr, and ultimately boosted wheat yield by 5.71% and 8.9%, respectively, in comparison with the non-Si-treated plants subjected to water stress at the anthesis and filling stages. However, the mitigating effect of Si application was not significant at the jointing stage. It was concluded that foliar supplementation of Si, especially at the reproductive stage, was effective in alleviating drought-induced yield reduction.

Accurate and timely appraisal of plant nitrogen (N) demand is imperative to regulate the canopy structure and corn production. The strength and time of plant N deficit can be quantified by critical N concentration. The study was aimed to analyze nitrogen nutrition index (NNI), nitrogen deficit content (NDC), plant nitrogen productivity (PNP), and a fraction of intercepted photosynthetic active radiation (FIPAR) across different N treatments and to develop NNI-NDC, NNI-PNP, NNI-FIPAR, NDC-PNP, and NDC-FIPAR relationships from V6 to V12 stages of corn to quantify the suitable PNP and FIPAR values under the optimal plant N condition. Four multi-N rates (0, 75, 90, 150, 180, 225, 270, and 300 kg N ha−1) field experiments were conducted with two cultivars of corn in Henan province of China. Results indicated that N fertilization affected yield, plant biomass, plant N content, and leaf area index. The values of NNI and NDC were from 0.54 to 1.28 kg ha−1 and from −28.13 to 21.99 kg ha−1 under the different treatments of N rate, respectively. The NDC and NNI showed significantly negative relationships from V6 to V12 stages. The values of PNP and FIPAR increased gradually with the crop growth process. The PNP values gradually declined while the FIPAR values of every leaf layer increased with the increase of N supply. The NDC-PNP and NNI-FIPAR relationships were significantly positive; however, the relationships between NNI-PNP and NDC-FIPAR were significantly negative during the vegetative period of corn. The coefficient of determination (R2) based on NNI was better than that on NDC. The FIPAR values were ~0.35, 0.67, and 0.76% at the upper, middle, and bottom of leaf layers, respectively, and PNP values were ~39, 44, and 51 kg kg−1 at V6, V9, and V12 stages, respectively, when NNI and NDC values were equal to 1 and 0 kg ha−1, respectively. This study described the quantitative information about the effect of a plant's internal N deficit on plant N productivity and canopy light intercept. The projected results would assist in predicting the appropriate plant growth status during key N top-dressing stages of corn, which can optimize N application and improve N use efficiency.

Optimizing planting structure that balances both high yield and water resources shortage is essential for developing efficient water-saving agriculture. To provide insights about the relationship between planting structure optimization and water resource constraint, crop water requirement, precipitation coupling degree, gross total water requirement and irrigation project metrics were calculated and analyzed with the dataset collected from 16 locations in Xuchang City, China. The strategy of reducing the planting proportion of high water-consumption crops and increasing low consumption and high precipitation coupling degree crops was adopted to determine a suitable water-saving planting scheme based on the IQR (interquartile range) method. Evapotranspiration had a decreasing trend from northwest to southeast areas. There were positive correlations between gross total water requirement (GTWR) and annual total yields (r = 0.825, p = 0.002), and between GTWR and proportion of vegetable planting areas (PVPA) (r = 0.734, p = 0.0101). The GTWR was negatively correlated with the ratio of water-saving irrigated areas to effective irrigated areas (RSEA), proportion of wheat planting areas (PWPA) and proportion of bean planting areas (PBPA), with coefficients of −0.787, −0.936 and −0.828, respectively. The planting proportion of winter wheat, summer maize, vegetables and flowers decreased by 8.8%, 25.8%, 16.2%, and 28.7%, respectively, while oil-beans and tubers increased by 62.4% and 95.6%, respectively. The irrigation water consumption was reduced by 5.2%, saving 3.25 × 107 m3 irrigation water without sacrificing economic benefits after adjusting for the whole region. Consequently, precipitation coupling degree, water-saving technology and historical planting habits should be considered when optimizing cropping distributions. This research provided a new theoretical basis and comprehensive approach for agriculture irrigation water management and regional planting structure optimization from a realistic perspective.

The non-destructive estimation of plant nitrogen (N) status is imperative for timely and in-season crop N management. The objectives of this study were to use canopy cover (CC) to establish the empirical relations between plant growth indices [shoot dry matter (SDM), leaf area index (LAI), shoot N accumulation (SNA), shoot nitrogen concentration (SNC)], and CC as well as to test the feasibility of using CC to assess N nutrition index (NNI) from Feekes 3 to Feekes 6 stages of winter wheat. Four multi-locational (2 sites), multi-cultivars (four cultivars), and multi-N rates (0–300 kg N ha–1) field experiments were carried out during 2016 to 2018 seasons. The digital images of the canopy were captured by a digital camera from Feekes 3 to Feekes 6 stages of winter wheat, while SDM, LAI, SNA, and SNC were measured by destructive plant sampling. CC was calculated from digital images developed by self-programmed software. CC showed significant correlations with growth indices (SDM, LAI, and SNA) across the different cultivars and N treatments, except for SNC. However, the stability of these empirical models was affected by cultivar characteristics and N application rates. Plant N status of winter wheat was assessed using CC through two methods (direct and indirect methods). The direct and indirect methods failed to develop a unified linear regression to estimate NNI owing to the high dispersion of winter wheat SNC during its early growth stages. The relationships of CC with SDM, SNC and NNI developed at individual growth stages of winter wheat using both methods were highly significant. The relationships developed at individual growth stages did not need to consider the effect of N dilution process, yet their stability is influenced by cultivar characteristics. This study revealed that CC has larger limitation to be used as a proxy to manage the crop growth and N nutrition during the early growth period of winter wheat despite it is an easily measured index.

In the North China Plain, drought usually occurs during the interval between wheat harvest and maize sowing in normal and dry years. The first irrigation for maize plays a critical role in guaranteeing seed germination and grain yields. Using experimental data from Xinxiang in 2019 and survey data of 641 farmers from the North China Plain in 2020, this study adopts a cost-benefit analysis method to investigate the impacts of irrigation time and well depths on farmers’ costs and benefits in maize production. The results showed that farms with well depth > 120 m accounted for 49% of total farms, especially in Hebei Province, and 38% wells had low water yield < 2.7 m3 kW−1 h−1. Delaying the time of the first irrigation made maize yields decline by up to 307 kg ha−1 day−1. Well depths increased irrigation costs and total maize production cost in an exponential manner, causing farmers’ benefits to decrease exponentially with well depths. With well depth > 180 m, the proportion of irrigation cost to total cost rose to 14%, whereas well depth > 230 m directly caused the farmers’ profits negative. A critical well depth of 230 m was put forward as the upper limit for farmers adopting maize planting in the NCP. The concept of ‘rotational irrigation strategy’ and suggestions of adopting drip irrigation, sprinkler irrigation, or hose-reel sprinkler irrigation were recommended to advance 6–8 days for the first irrigation period, compared with traditional flood irrigation.

Summer maize crop development, yield, and water use characteristics under water deficit conditions at different growth stages were investigated in this study using different irrigation regime treatments at the seedling (S), jointing (J), tasseling (T), and grain filling stages (F) in 2018 and 2019 in China. Ten different irrigation treatments were set, including three-irrigation application intervals (JTFi, STFi, SJFi, SJTi), two-irrigation applications (STi, JTi, JFi), and single-irrigation applications (Ti, Ji). These were compared to the control treatment (CK), which had sufficient irrigation provided at four intervals (SJTFi). The results showed that compared to CK, a water deficit at the seedling and jointing stages had a greater effect on plant height, whereas a water deficit at the tasseling and filling stages had a greater effect on the leaf area index, and a continuous water deficit had an effect on the stem diameter of summer maize. Limitations in terms of the growth and development of summer maize increased with less frequent irrigation. As irrigation decreased, the grain yield decreased, and the water use efficiency increased, and a water deficit at the tasseling stage had the greatest effect on the yield and water use efficiency. The JTFi treatment was the optimal irrigation regime with a yield decline, and its water consumption was reduced by 16.9% (p < 0.05) on average. However, compared to CK, the water use efficiency of the JTFi treatment increased by 17.3% (p < 0.05). Moreover, the JTFi treatment had the smallest maize yield response factor value (Ky) of 0.16, and its comprehensive score was the second highest after CK.

The temperature-based crop water stress index (CWSI) can accurately reflect the extent of crop water deficit. As an ideal carrier of onboard thermometers to monitor canopy temperature (Tc), center pivot irrigation systems (CPIS) have been widely used in precision irrigation. However, the determination of reliable CWSI thresholds for initiating the CPIS is still a challenge for a winter wheat–summer maize cropping system in the North China Plain (NCP). To address this problem, field experiments were carried out to investigate the effects of CWSI thresholds on grain yield (GY) and water use efficiency (WUE) of winter wheat and summer maize in the NCP. The results show that positive linear functions were fitted to the relationships between CWSI and canopy minus air temperature (Tc − Ta) (r2 > 0.695), and between crop evapotranspiration (ETc) and Tc (r2 > 0.548) for both crops. To make analysis comparable, GY and WUE data were normalized to a range of 0.0 to 1.0, corresponding the range of CWSI. With the increase in CWSI, a positive linear relationship was observed for WUE (r2 = 0.873), while a significant inverse relationship was found for the GY (r2 = 0.915) of winter wheat. Quadratic functions were fitted for both the GY (r2 = 0.856) and WUE (r2 = 0.629) of summer maize. By solving the cross values of the two GY and WUE functions for each crop, CWSI thresholds were proposed as being 0.322 for winter wheat, and 0.299 for summer maize, corresponding to a Tc − Ta threshold value of 0.925 and 0.498 °C, respectively. We conclude that farmers can achieve the dual goals of high GY and high WUE using the optimal thresholds proposed for a winter wheat–summer maize cropping system in the NCP.

Agricultural soils are major sources of the greenhouse gas N2O, particularly under maize with high nitrogen (N) inputs and excessive irrigation. To determine how N and water management can mitigate N2O emissions from drip-irrigated maize fields, a field study was carried out in the North China Plain during the maize growing season. The objective of the study was to investigate the individual and combined effects of irrigation level and N input on N2O emissions and grain yields, as well as N and water use efficiency (WUE), under drip-fertigation management. The main treatments were two irrigation levels: 50 mm/event (sufficient irrigation, WH) and 25 mm/event (limited irrigation, WL), with four N application rates: 0 (N0), 90 (N1), 180 (N2), and 270 (N3) kg N ha−1. Pulses of N2O emissions were recorded every 2–3 d following each irrigation or fertigation (irrigation + fertilization) event. N input, irrigation, and their interactions all significantly influenced N2O emissions. A significant exponential relationship (P

Silicon (Si) enhances maize resistance to drought. While previous studies have mainly focused on the seedling stage, the mediation of drought stress by Si imposed at the vegetative and reproductive stages has been rarely investigated. A soil-column experiment was thus conducted under a rainproof shelter to quantify the effect s of Si application on the physiological and agronomic responses of maize to drought stress imposed at the 6-leaf (D-V6), 12-leaf (D-V12), and blister (D-R2) stages. The observed parameters included plant growth, photosynthesis, osmolytes, antioxidant activity, and grain yield. The results showed that drought stress strongly decreased the leaf area, leaf water content, photosynthetic rate, chlorophyll content, and antioxidant activity (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)) and markedly increased lipid peroxidation. D-V6, D-V12, and D-R2 decreased grain yields by 12.9%, 28.9%, and 44.8%, respectively, compared to the well-watered treatment (CK). However, Si application markedly increased leaf area, chlorophyll content, photosynthetic rate, osmolyte content, and enzymatic antioxidant activities (SOD, POD, and CAT), and decreased malondialdehyde (MDA) and superoxide radical accumulation, ultimately improving maize yields by 12.4%, 69.8%, and 80.8%, respectively, compared to the non-Si treated plants under drought stress at the V6, V12, and R2 stages. Furthermore, maize yields had a significant positive correlation with chlorophyll content and SOD and POD activity during the three stages. Our findings suggest that Si-induced changes in chlorophyll content and antioxidant activity might constitute important mechanisms for mitigating drought stress. In conclusion, this study provides physico-biochemical evidence for the beneficial role of Si in alleviating drought-induced yield reduction in maize, particularly during the late vegetative or early reproductive stages. Thus, Si application constitutes an effective approach for improving maize yield in rain-fed agricultural systems.

Rapid and non-destructive diagnostic tools to accurately assess crop nitrogen nutrition index (NNI) are imperative for improving crop nitrogen (N) diagnosis and sustaining crop production. This study was aimed to develop the relationships among NNI, leaf N gradient, chlorophyll meter (CM) readings gradient, and positional differences chlorophyll meter index [PDCMI, the ratio of CM readings between different leaf layers (LLs) of crop canopy] and to validate the accuracy and stability of these relationships across the different LLs, years, sites, and cultivars. Six multi-N rates (0–320 kg ha−1) field experiments were conducted with four summer maize cultivars (Zhengdan958, Denghai605, Xundan20, and Denghai661) at two different sites located in China. Six summer maize plants per plot were harvested at each sampling stage to assess NNI, leaf N concentration and CM readings of different LLs during the vegetative growth period. The results showed that the leaf N gradient, CM readings gradient and PDCMI of different LLs decreased, while the NNI values increased with increasing N supply. The leaf N gradient and CM readings gradient increased gradually from top to bottom of the canopy and CM readings of the bottom LL were more sensitive to changes in plant N concentration. The significantly positive relationship between NNI and CM readings of different LLs (LL1 to LL3) was observed, yet these relationships varied across the years. In contrast, the relationships between NNI and PDCMI of different LLs (LL1 to LL3) were significantly negative. The strongest relationship between PDCMI and NNI which was stable across the cultivars and years was observed for PDCMI1−3 (NNI = −5.74 × PDCMI1−3+1.5, R2 = 0.76**). Additionally, the models developed in this study were validated with the data acquired from two independent experiments to assess their accuracy of prediction. The root mean square error value of 0.1 indicated that the most accurate and robust relationship was observed between PDCMI1–3 and NNI. The projected results would help to develop a simple, non-destructive and reliable approach to accurately assess the crop N status for precisely managing N application during the growth period of summer maize crop.

Long-term tillage has been shown to induce water stress episode during crop growth period due to low water retention capacity. It is unclear whether integrated water conservation tillage systems, such asspringdeepinter-row subsoiling with annual or biennial repetitions, can be developed to alleviate this issue while improve crop productivity.Experimentswere carried out in a spring maize cropping system on Calcaric-fluvicCambisolsatJiaozuoexperimentstation, northern China, in 2009 to 2014. Effects of threesubsoiling depths (i.e., 30 cm, 40 cm, and 50 cm) in combination with annual and biennial repetitionswasdetermined in two single-years (i.e., 2012 and 2014)againstthe conventional tillage. The objectives were to investigateyield response to subsoiling depths and soil water deficit(SWD), and to identify the most effective subsoiling treatment using a systematic assessment.Annualsubsoiling to 50 cm (AS-50) increased soil water storage (SWS, mm) by an average of8% in 0-20 cm soil depth, 19% in 20-80 cm depth, and 10% in 80-120 cm depth, followed by AS-40 and BS-50, whereas AS-30 and BS-30 showed much less effects in increasing SWS across the 0-120 cm soil profile, compared to the CK. AS-50 significantly reduced soil water deficit (SWD, mm) by an average of123% during sowing to jointing, 318% during jointing to filling, and 221% during filling to maturity, compared to the CK, followed by AS-40 and BS-50. An integrated effect on increasing SWS and reducing SWD helped AS-50 boost grain yield by an average of 31% and biomass yield by 30%, compared to the CK. A power function for subsoiling depth and a negative linear function for SWD were used to fit the measured yields, showing the deepest subsoiling depth (50 cm) with the lowest SWD contributed to the highest yield. Systematic assessment showed that AS-50 received the highest evaluation index (0.69 out of 1.0) among all treatments.Deepinter-row subsoilingwith annual repetition significantly boosts yield by alleviating SWD in critical growth period and increasing SWS in 20-80 cm soil depth. The results allow us to conclude that AS-50 can be adopted as an effective approach to increase crop productivity, alleviate water stress, and improve soil water availability for spring maize in northern China.

Slag-based silicate fertilizer has been widely used to improve soil silicon- availability and crop productivity. A consecutive early rice-late rice rotation experiment was conducted to test the impacts of steel slag on soil pH, silicon availability, rice growth and metals-immobilization in paddy soil. Our results show that application of slag at a rate above higher or equal to 1 600 mg plant-available SiO2 per kg soil increased soil pH, dry weight of rice straw and grain, plant-available Si concentration and Si concentration in rice shoots compared with the control treatment. No significant accumulation of total cadmium (Cd) and lead (Pb) was noted in soil; rather, the exchangeable fraction of Cd significantly decreased. The cadmium concentrations in rice grains decreased significantly compared with the control treatment. In conclusion, application of steel slag reduced soil acidity, increased plant-availability of silicon, promoted rice growth and inhibited Cd transport to rice grain in the soil-plant system.

It is well documented that slag-based silicon fertilizers have beneficial effects on the growth and disease resistance of rice. However, their effects vary greatly with sources of slag and are closely related to availability of silicon (Si) in these materials. To date, few researches have been done to compare the differences in plant performance and disease resistance between different slag-based silicon fertilizers applied at the same rate of plant-available Si. In the present study both steel and iron slags were chosen to investigate their effects on rice growth and disease resistance under greenhouse conditions. Both scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the effects of slags on ultrastructural changes in leaves of rice naturally infected by Bipolaris oryaze, the causal agent of brown spot. The results showed that both slag-based Si fertilizers tested significantly increased rice growth and yield, but decreased brown spot incidence, with steel slag showing a stronger effect than iron slag. The results of SEM analysis showed that application of slags led to more pronounced cell silicification in rice leaves, more silica cells, and more pronounced and larger papilla as well. The results of TEM analysis showed that mesophyll cells of slag-untreated rice leaf were disorganized, with colonization of the fungus (Bipolaris oryzae), including chloroplast degradation and cell wall alterations. The application of slag maintained mesophyll cells relatively intact and increased the thickness of silicon layer. It can be concluded that applying slag-based fertilizer to Si-deficient paddy soil is necessary for improving both rice productivity and brown spot resistance. The immobile silicon deposited in host cell walls and papillae sites is the first physical barrier for fungal penetration, while the soluble Si in the cytoplasm enhances physiological or induced resistance to fungal colonization.