1. Osteoinductive 3D scaffolds prepared by blend centrifugal spinning for long-term delivery of osteogenic supplements
- Author
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Sovkova Vera, Rustichelli Franco, Lukasova Vera, Doupník Miroslav, Staffa Andrea, Litvinec Andrej, Filova Eva, Voltrová Barbora, Tonar Zbyněk, Kralickova Milena, Kubíková Tereza, Blahnová Veronika, Amler Evzen, Rampichova Michala, Vocetková Karolína, Buzgo Matej, and Svora Petr
- Subjects
0301 basic medicine ,Scaffold ,Chemistry ,General Chemical Engineering ,Bone implant ,Mesenchymal stem cell ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,03 medical and health sciences ,030104 developmental biology ,In vivo ,Drug delivery ,Term delivery ,0210 nano-technology ,Bone regeneration ,Spinning ,Biomedical engineering - Abstract
Bone regeneration is a long-term process requiring proper scaffolding and drug delivery systems. The current study delivers a three-dimensional (3D) scaffold prepared by blend centrifugal spinning loaded with the osteogenic supplements (OS) β-glycerol phosphate, ascorbate-2-phosphate and dexamethasone. The OS were successfully encapsulated into a fibrous scaffold and showed sustained release for 30 days. Furthermore, biological testing showed the osteoinductive properties of the scaffolds on a model of human mesenchymal stem cells and stimulatory effect on a model of osteoblasts. The osteoinductive properties were further proved in vivo in critical size defects of rabbits. The amount of bone trabecules was bigger compared to control fibers without OS. The results indicate that due to its long-term drug releasing properties, single step fabrication process and 3D structure, the system shows ideal properties for use as a cell-free bone implant in tissue-engineering.
- Published
- 2018
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