1. A preliminary study to identify and distinguish southern tropical populations of Ganoderma boninense from oil palm via mating assays, sequence data, and microsatellite markers
- Author
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Derick Rama, Emmanuel A Gorea, C. A. Pilotti, Agnieszka M. Mudge, and Gini Killah
- Subjects
biology ,Physiology ,Zoology ,Cell Biology ,General Medicine ,Elaeis guineensis ,biology.organism_classification ,Gene flow ,parasitic diseases ,Genetics ,Microsatellite ,Mating ,Internal transcribed spacer ,Stem rot ,Molecular Biology ,Genotyping ,Inbreeding ,Ecology, Evolution, Behavior and Systematics - Abstract
Morphological studies suggest that the major pathogen causing basal stem rot of oil palm in Papua New Guinea and Solomon Islands is Ganoderma boninense. This study presents the first evidence for conspecificity of G. boninense from four countries where basal stem rot is prevalent. Seventy-three dikaryotic isolates of Ganoderma boninense from Indonesia, Malaysia, Papua New Guinea, and Solomon Islands were studied via mating tests, analyses of nuc internal transcribed spacer ITS1-5.8S-ITS2 sequences, and microsatellite genotyping. Sequence similarity in the ITS1-5.8S-ITS2 region was >99%, and all exotic isolates successfully mated with Papua New Guinea tester strains. Transfer of nuclei during mating was also confirmed via microsatellite markers for the first time in this species. Four microsatellite primers were used to generate evidence for 33 alleles in the four populations. All isolates studied had unique genetic fingerprints but alleles were also shared, suggesting gene flow. Heterozygosities were lower than expected in Indonesian and Papua New Guinea populations, consistent with the possibility of localized inbreeding.
- Published
- 2021