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10. Additional file 6 of Pathogen and drought stress affect cell wall and phytohormone signaling to shape host responses in a sorghum COMT bmr12 mutant

Author

Khasin, Maya, Bernhardson, Lois F., O’Neill, Patrick M., Palmer, Nathan A., Scully, Erin D., Sattler, Scott E., and Funnell-Harris, Deanna L.

Subjects
congenital, hereditary, and neonatal diseases and abnormalities, education, fungi, nutritional and metabolic diseases, food and beverages
Abstract

Additional file 6 Time to bloom per genotype. The bmr12 mutant plants exhibit delayed bloom, resulting in their disproportionate culling from the experiment, as plants were inoculated after bloom, resulting in disproportionate missing data from bmr12 plants.

Published
2021
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11. Additional file 8 of Pathogen and drought stress affect cell wall and phytohormone signaling to shape host responses in a sorghum COMT bmr12 mutant

Author

Khasin, Maya, Bernhardson, Lois F., O’Neill, Patrick M., Palmer, Nathan A., Scully, Erin D., Sattler, Scott E., and Funnell-Harris, Deanna L.

Abstract

Additional file 8. Principle component analysis (PCA) of samples indicating significant separation by day of sampling (A), which is confounded with RNA sequencing run, and little separation based on water treatment (B). Separated by day of run (C), the separation between treatment conditions is clarified.

Published
2021
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13. Tri5 gene expression analysis during postharvest storage of wheat grain from field plots treated with a triazole and a strobilurin fungicide

Author

Bolanos-Carriel, Carlos, Wegulo, Stephen N., Baenziger, P. Stephen, Eskridge, Kent M., Funnell-Harris, Deanna, McMaster, Nicole J., Schmale, David G. III, Hallen-Adams, Heather E., and School of Plant and Environmental Sciences

Subjects
triazole, Fusarium head blight, wheat, deoxynivalenol, gene expression, food and beverages, trichodiene synthase, strobilurin
Abstract

Fusarium head blight (FHB) and the associated mycotoxin deoxynivalenol (DON) negatively impact the wheat industry worldwide. In North America, FHB is mainly caused by Fusarium graminearum sensu stricto. The purpose of this study was to evaluate, under storage conditions, the expression of the DON biosynthetic gene trichodiene synthase (Tri5) of F. graminearum in grain of hard red winter wheat cultivars 'Overley' (FHB-susceptible) and 'Overland' (moderately resistant to FHB) from field plots treated or untreated with the triazole fungicide Prosaro and the strobilurin fungicide Headline. Infected grain was stored and periodically sampled to determine gene expression by qRT-PCR analysis. The F. graminearum housekeeping gene GAPDH was consistently detected, indicative of metabolically active fungi, and Tri5 detection was significantly higher in 'Overley' compared with 'Overland'. Tri5 gene expression and DON concentrations showed little to no correlation; consequently, Tri5 expression levels did not accurately predict DON concentrations. The strobilurin did not significantly reduce Tri5 gene expression compared with untreated wheat. In the triazole treatment, a significant reduction in the relative expression of Tri5 was detected after 120 days, as well as a downregulation of Tri5 from 60 to 120 days of storage in 'Overley'. In grain from strobilurin-treated plots of both cultivars, the expression of Tri5 increased from 0 to 30 days after tempering. Genetic expression of Tri5 that is necessary for the production of DON can increase during storage of high-moisture grain. Fusarium fungi can persist in wheat kernels for several months postharvest and may actively produce toxin during this period. U.S. Department of AgricultureUnited States Department of Agriculture (USDA); Secretariat of Higher Education, Science, Technology and Innovation of Ecuador; Nebraska Wheat Board; U.S. Wheat and Barley Scab InitiativeUnited States Department of Agriculture (USDA) [59-0790-7-080, 422288, 422533]; Hatch Multistate Research Capacity Funding Program [1006715] This work was supported by the U.S. Department of Agriculture; The Secretariat of Higher Education, Science, Technology and Innovation of Ecuador; Nebraska Wheat Board; U.S. Wheat and Barley Scab Initiative [59-0790-7-080; #422288; #422533]; Hatch Multistate Research Capacity Funding Program [1006715]. Public domain – authored by a U.S. government employee

Published
2020

22. Overexpression of SbMyb60 in Sorghum bicolor impacts both primary and secondary metabolism.

Author

Scully, Erin D., Gries, Tammy, Palmer, Nathan A., Sarath, Gautam, Funnell‐Harris, Deanna L., Baird, Lisa, Twigg, Paul, Seravalli, Javier, Clemente, Thomas E., and Sattler, Scott E.

Subjects
GENETIC overexpression, TRANSCRIPTION factors, BIOSYNTHESIS, SORGHUM, SORGHUM genetics
Abstract

Few transcription factors have been identified in C4 grasses that either positively or negatively regulate monolignol biosynthesis., Previously, the overexpression of SbMyb60 in sorghum ( Sorghum bicolor) has been shown to induce monolignol biosynthesis, which leads to elevated lignin deposition and altered cell wall composition. To determine how SbMyb60 overexpression impacts other metabolic pathways, RNA-Seq and metabolite profiling were performed on stalks and leaves., 35S::SbMyb60 was associated with the transcriptional activation of genes involved in aromatic amino acid, S-adenosyl methionine ( SAM) and folate biosynthetic pathways. The high coexpression values between SbMyb60 and genes assigned to these pathways indicate that SbMyb60 may directly induce their expression. In addition, 35S::SbMyb60 altered the expression of genes involved in nitrogen (N) assimilation and carbon (C) metabolism, which may redirect C and N towards monolignol biosynthesis. Genes linked to UDP-sugar biosynthesis and cellulose synthesis were also induced, which is consistent with the observed increase in cellulose deposition in the internodes of 35S::SbMyb60 plants. However, SbMyb60 showed low coexpression values with these genes and is not likely to be a direct regulator of cell wall polysaccharide biosynthesis., These findings indicate that SbMyb60 can activate pathways beyond monolignol biosynthesis, including those that synthesize the substrates and cofactors required for lignin biosynthesis. [ABSTRACT FROM AUTHOR]

Published
2018
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27. Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum.

Author

Scully, Erin D., Gries, Tammy, Funnell‐Harris, Deanna L., Xin, Zhanguo, Kovacs, Frank A., Vermerris, Wilfred, and Sattler, Scott E.

Subjects
PLANT mutation, CROP yields, SORGHUM, LIGNINS, BIOSYNTHESIS, LIGNOCELLULOSE, PLANT biomass, FORAGE plant quality
Abstract

The presence of lignin reduces the quality of lignocellulosic biomass for forage materials and feedstock for biofuels. In C4 grasses, the brown midrib phenotype has been linked to mutations to genes in the monolignol biosynthesis pathway. For example, the Bmr6 gene in sorghum ( Sorghum bicolor) has been previously shown to encode cinnamyl alcohol dehydrogenase (CAD), which catalyzes the final step of the monolignol biosynthesis pathway. Mutations in this gene have been shown to reduce the abundance of lignin, enhance digestibility, and improve saccharification efficiencies and ethanol yields. Nine sorghum lines harboring five different bmr6 alleles were identified in an EMS-mutagenized TILLING population. DNA sequencing of Bmr6 revealed that the majority of the mutations impacted evolutionarily conserved amino acids while three-dimensional structural modeling predicted that all of these alleles interfered with the enzyme's ability to bind with its NADPH cofactor. All of the new alleles reduced in vitro CAD activity levels and enhanced glucose yields following saccharification. Further, many of these lines were associated with higher reductions in acid detergent lignin compared to lines harboring the previously characterized bmr6-ref allele. These bmr6 lines represent new breeding tools for manipulating biomass composition to enhance forage and feedstock quality. [ABSTRACT FROM AUTHOR]

Published
2016
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