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1. Tailoring Plasmonic Nanoheaters Size for Enhanced Theranostic Agent Performance

Author

Túlio de L. Pedrosa, Gabrielli M. F. de Oliveira, Arthur C. M. V. Pereira, Mariana J. B. da S. Crispim, Luzia A. da Silva, Marcilene S. da Silva, Ivone A. de Souza, Ana M. M. de A. Melo, Anderson S. L. Gomes, and Renato E. de Araujo

Subjects
gold nanoparticles, photoacoustic imaging, photothermal therapy, size optimization, S180 cells, Technology, Biology (General), QH301-705.5
Abstract

The introduction of optimized nanoheaters, which function as theranostic agents integrating both diagnostic and therapeutic processes, holds significant promise in the medical field. Therefore, developing strategies for selecting and utilizing optimized plasmonic nanoheaters is crucial for the effective use of nanostructured biomedical agents. This work elucidates the use of the Joule number (Jo) as a figure of merit to identify high-performance plasmonic theranostic agents. A framework for optimizing metallic nanoparticles for heat generation was established, uncovering the size dependence of plasmonic nanoparticles optical heating. Gold nanospheres (AuNSs) with a diameter of 50 nm and gold nanorods (AuNRs) with dimensions of 41×10 nm were identified as effective nanoheaters for visible (530 nm) and infrared (808 nm) excitation. Notably, AuNRs achieve higher Jo values than AuNSs, even when accounting for the possible orientations of the nanorods. Theoretical results estimate that 41×10 nm gold nanorods have an average Joule number of 80, which is significantly higher compared to larger rods. The photothermal performance of optimal and suboptimal nanostructures was evaluated using photoacoustic imaging and photothermal therapy procedures. The photoacoustic images indicate that, despite having larger absorption cross-sections, the large nanoparticle volume of bigger particles leads to less efficient conversion of light into heat, which suggests that the use of optimized nanoparticles promotes higher contrast, benefiting photoacoustic-based procedures in diagnostic applications. The photothermal therapy procedure was performed on S180-bearing mice inoculated with 41×10 nm and 90×25 nm PEGylated AuNRs. Five minutes of laser irradiation of tumor tissue with 41×10 nm produced an approximately 9.5% greater temperature rise than using 90×25 AuNRs in the therapy trials. Optimizing metallic nanoparticles for heat generation may reduce the concentration of the nanoheaters used or decrease the light fluence for bioscience applications, paving the way for the development of more economical theranostic agents.

Published
2024
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4. Tailoring Plasmonic Nanoheaters Size for Enhanced Theranostic Agent Performance.

Author

Pedrosa, Túlio de L., de Oliveira, Gabrielli M. F., Pereira, Arthur C. M. V., Crispim, Mariana J. B. da S., da Silva, Luzia A., da Silva, Marcilene S., de Souza, Ivone A., Melo, Ana M. M. de A., Gomes, Anderson S. L., and de Araujo, Renato E.

Subjects
GOLD nanoparticles, ACOUSTIC imaging, LIFE sciences, NANOPARTICLES, PLASMONICS, PHOTOTHERMAL effect
Abstract

The introduction of optimized nanoheaters, which function as theranostic agents integrating both diagnostic and therapeutic processes, holds significant promise in the medical field. Therefore, developing strategies for selecting and utilizing optimized plasmonic nanoheaters is crucial for the effective use of nanostructured biomedical agents. This work elucidates the use of the Joule number ( J o ) as a figure of merit to identify high-performance plasmonic theranostic agents. A framework for optimizing metallic nanoparticles for heat generation was established, uncovering the size dependence of plasmonic nanoparticles optical heating. Gold nanospheres (AuNSs) with a diameter of 50 nm and gold nanorods (AuNRs) with dimensions of 41 × 10 nm were identified as effective nanoheaters for visible (530 nm) and infrared (808 nm) excitation. Notably, AuNRs achieve higher J o values than AuNSs, even when accounting for the possible orientations of the nanorods. Theoretical results estimate that 41 × 10 nm gold nanorods have an average Joule number of 80, which is significantly higher compared to larger rods. The photothermal performance of optimal and suboptimal nanostructures was evaluated using photoacoustic imaging and photothermal therapy procedures. The photoacoustic images indicate that, despite having larger absorption cross-sections, the large nanoparticle volume of bigger particles leads to less efficient conversion of light into heat, which suggests that the use of optimized nanoparticles promotes higher contrast, benefiting photoacoustic-based procedures in diagnostic applications. The photothermal therapy procedure was performed on S180-bearing mice inoculated with 41 × 10 nm and 90 × 25 nm PEGylated AuNRs. Five minutes of laser irradiation of tumor tissue with 41 × 10 nm produced an approximately 9.5% greater temperature rise than using 90 × 25 AuNRs in the therapy trials. Optimizing metallic nanoparticles for heat generation may reduce the concentration of the nanoheaters used or decrease the light fluence for bioscience applications, paving the way for the development of more economical theranostic agents. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Human Umbilical Cord-Mesenchymal Stem Cells Promote Extracellular Matrix Remodeling in Microglia

Author

Lombardo, M, Gabrielli, M, Julien-Marsollier, F, Faivre, V, Le Charpentier, T, Bokobza, C, D'Aliberti, D, Pelizzi, N, Halimi, C, Spinelli, S, Van Steenwinckel, J, Verderio, E, Gressens, P, Piazza, R, Verderio, C, Lombardo, Marta Tiffany, Gabrielli, Martina, Julien-Marsollier, Florence, Faivre, Valérie, Le Charpentier, Tifenn, Bokobza, Cindy, D'Aliberti, Deborah, Pelizzi, Nicola, Halimi, Camilla, Spinelli, Silvia, Van Steenwinckel, Juliette, Verderio, Elisabetta A. M., Gressens, Pierre, Piazza, Rocco, Verderio, Claudia, Lombardo, M, Gabrielli, M, Julien-Marsollier, F, Faivre, V, Le Charpentier, T, Bokobza, C, D'Aliberti, D, Pelizzi, N, Halimi, C, Spinelli, S, Van Steenwinckel, J, Verderio, E, Gressens, P, Piazza, R, Verderio, C, Lombardo, Marta Tiffany, Gabrielli, Martina, Julien-Marsollier, Florence, Faivre, Valérie, Le Charpentier, Tifenn, Bokobza, Cindy, D'Aliberti, Deborah, Pelizzi, Nicola, Halimi, Camilla, Spinelli, Silvia, Van Steenwinckel, Juliette, Verderio, Elisabetta A. M., Gressens, Pierre, Piazza, Rocco, and Verderio, Claudia

Abstract

Human mesenchymal stem cells modulate the immune response and are good candidates for cell therapy in neuroinflammatory brain disorders affecting both adult and premature infants. Recent evidence indicates that through their secretome, mesenchymal stem cells direct microglia, brain-resident immune cells, toward pro-regenerative functions, but the mechanisms underlying microglial phenotypic transition are still under investigation. Using an in vitro coculture approach combined with transcriptomic analysis, we identified the extracellular matrix as the most relevant pathway altered by the human mesenchymal stem cell secretome in the response of microglia to inflammatory cytokines. We confirmed extracellular matrix remodeling in microglia exposed to the mesenchymal stem cell secretome via immunofluorescence analysis of the matrix component fibronectin and the extracellular crosslinking enzyme transglutaminase-2. Furthermore, an analysis of hallmark microglial functions revealed that changes in the extracellular matrix enhance ruffle formation by microglia and cell motility. These findings point to extracellular matrix changes, associated plasma membrane remodeling, and enhanced microglial migration as novel mechanisms by which mesenchymal stem cells contribute to the pro-regenerative microglial transition.

Published
2024

6. Adsorption Capabilities of Fungoid Chitosan Toward Organic Acids in Model Solutions and White Wine

Author

Gruppi, Alice, Romanini, Elia, Bassani, Andrea, Lambri, Milena, Chinnici, F., Gabrielli, Mario, Gruppi A., Romanini E., Bassani A. (ORCID:0000-0001-8258-4369), Lambri M. (ORCID:0000-0003-4330-2888), Gabrielli M. (ORCID:0000-0002-9313-0140), Gruppi, Alice, Romanini, Elia, Bassani, Andrea, Lambri, Milena, Chinnici, F., Gabrielli, Mario, Gruppi A., Romanini E., Bassani A. (ORCID:0000-0001-8258-4369), Lambri M. (ORCID:0000-0003-4330-2888), and Gabrielli M. (ORCID:0000-0002-9313-0140)

Published
2024

7. Paired plasma lipidomics and proteomics analysis in the conversion from mild cognitive impairment to Alzheimer's disease

Author

Gómez-Pascual, A, Naccache, T, Xu, J, Hooshmand, K, Wretlind, A, Gabrielli, M, Lombardo, M, Shi, L, Buckley, N, Tijms, B, Vos, S, Ten Kate, M, Engelborghs, S, Sleegers, K, Frisoni, G, Wallin, A, Lleó, A, Popp, J, Martinez-Lage, P, Streffer, J, Barkhof, F, Zetterberg, H, Visser, P, Lovestone, S, Bertram, L, Nevado-Holgado, A, Gualerzi, A, Picciolini, S, Proitsi, P, Verderio, C, Botía, J, Legido-Quigley, C, Gómez-Pascual, Alicia, Naccache, Talel, Xu, Jin, Hooshmand, Kourosh, Wretlind, Asger, Gabrielli, Martina, Lombardo, Marta Tiffany, Shi, Liu, Buckley, Noel J, Tijms, Betty M, Vos, Stephanie J B, Ten Kate, Mara, Engelborghs, Sebastiaan, Sleegers, Kristel, Frisoni, Giovanni B, Wallin, Anders, Lleó, Alberto, Popp, Julius, Martinez-Lage, Pablo, Streffer, Johannes, Barkhof, Frederik, Zetterberg, Henrik, Visser, Pieter Jelle, Lovestone, Simon, Bertram, Lars, Nevado-Holgado, Alejo J, Gualerzi, Alice, Picciolini, Silvia, Proitsi, Petroula, Verderio, Claudia, Botía, Juan A, Legido-Quigley, Cristina, Gómez-Pascual, A, Naccache, T, Xu, J, Hooshmand, K, Wretlind, A, Gabrielli, M, Lombardo, M, Shi, L, Buckley, N, Tijms, B, Vos, S, Ten Kate, M, Engelborghs, S, Sleegers, K, Frisoni, G, Wallin, A, Lleó, A, Popp, J, Martinez-Lage, P, Streffer, J, Barkhof, F, Zetterberg, H, Visser, P, Lovestone, S, Bertram, L, Nevado-Holgado, A, Gualerzi, A, Picciolini, S, Proitsi, P, Verderio, C, Botía, J, Legido-Quigley, C, Gómez-Pascual, Alicia, Naccache, Talel, Xu, Jin, Hooshmand, Kourosh, Wretlind, Asger, Gabrielli, Martina, Lombardo, Marta Tiffany, Shi, Liu, Buckley, Noel J, Tijms, Betty M, Vos, Stephanie J B, Ten Kate, Mara, Engelborghs, Sebastiaan, Sleegers, Kristel, Frisoni, Giovanni B, Wallin, Anders, Lleó, Alberto, Popp, Julius, Martinez-Lage, Pablo, Streffer, Johannes, Barkhof, Frederik, Zetterberg, Henrik, Visser, Pieter Jelle, Lovestone, Simon, Bertram, Lars, Nevado-Holgado, Alejo J, Gualerzi, Alice, Picciolini, Silvia, Proitsi, Petroula, Verderio, Claudia, Botía, Juan A, and Legido-Quigley, Cristina

Abstract

Background: Alzheimer's disease (AD) is a neurodegenerative condition for which there is currently no available medication that can stop its progression. Previous studies suggest that mild cognitive impairment (MCI) is a phase that precedes the disease. Therefore, a better understanding of the molecular mechanisms behind MCI conversion to AD is needed. Method: Here, we propose a machine learning-based approach to detect the key metabolites and proteins involved in MCI progression to AD using data from the European Medical Information Framework for Alzheimer's Disease Multimodal Biomarker Discovery Study. Proteins and metabolites were evaluated separately in multiclass models (controls, MCI and AD) and together in MCI conversion models (MCI stable vs converter). Only features selected as relevant by 3/4 algorithms proposed were kept for downstream analysis. Results: Multiclass models of metabolites highlighted nine features further validated in an independent cohort (0.726 mean balanced accuracy). Among these features, one metabolite, oleamide, was selected by all the algorithms. Further in-vitro experiments in rodents showed that disease-associated microglia excreted oleamide in vesicles. Multiclass models of proteins stood out with nine features, validated in an independent cohort (0.720 mean balanced accuracy). However, none of the proteins was selected by all the algorithms. Besides, to distinguish between MCI stable and converters, 14 key features were selected (0.872 AUC), including tTau, alpha-synuclein (SNCA), junctophilin-3 (JPH3), properdin (CFP) and peptidase inhibitor 15 (PI15) among others. Conclusions: This omics integration approach highlighted a set of molecules associated with MCI conversion important in neuronal and glia inflammation pathways.

Published
2024

9. Hematopoietic Stem Cell Transplantation in Late-onset X-linked Chronic Granulomatous Disease in a Female Carrier

Author

Trevisan M, Kang EM, Salton F, Ruaro B, Torregiani C, Confalonieri P, Naviglio S, Valencic E, Gabrielli M, Parta M, Kelly C, Notarangelo LD, Malech HL, Tommasini A, Confalonieri M., Trevisan, M, Kang, Em, Salton, F, Ruaro, B, Torregiani, C, Confalonieri, P, Naviglio, S, Valencic, E, Gabrielli, M, Parta, M, Kelly, C, Notarangelo, Ld, Malech, Hl, Tommasini, A, and Confalonieri, M.

Subjects
Interferon-gamma, Transplantation Conditioning, Immunology, Hematopoietic Stem Cell Transplantation, Humans, Immunology and Allergy, Female, Chronic Granulomatous Disease, Granulomatous Disease, Chronic, Article
Abstract

Chronic granulomatous disease (CGD) is a rare immunodeficiency disorder characterized by a defective NADPH oxidase complex leading to an impaired respiratory burst and defective killing of pathogens by phagocytes. The most common type of CGD is caused by hemizygous mutations of the CYBB gene on the X chromosome encoding the gp91phox subunit. It usually affects males, yet heterozygous females may rarely manifest clinical signs of the disease due to skewed X chromosome inactivation in leukocytes . Despite advances in gene therapy, hematopoietic stem cell transplantation (HSCT) remains the main definitive treatment. Older patients, however, are at greater risk of HSCT-related complications and mortality and may require a specific approach.

Published
2022
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10. Identifying the best parameters to determine genotype capability to retain adequate malic acid at harvest and in final wines

Author

Frioni, Tommaso, Collivasone, Riccardo, Canavera, Ginevra, Gatti, Matteo, Gabrielli, Mario, Poni, Stefano, Frioni T. (ORCID:0000-0002-8663-3022), Collivasone R., Canavera G. (ORCID:0000-0002-4631-7359), Gatti M. (ORCID:0000-0003-4195-7709), Gabrielli M. (ORCID:0000-0002-9313-0140), Poni S. (ORCID:0000-0002-7238-2613), Frioni, Tommaso, Collivasone, Riccardo, Canavera, Ginevra, Gatti, Matteo, Gabrielli, Mario, Poni, Stefano, Frioni T. (ORCID:0000-0002-8663-3022), Collivasone R., Canavera G. (ORCID:0000-0002-4631-7359), Gatti M. (ORCID:0000-0003-4195-7709), Gabrielli M. (ORCID:0000-0002-9313-0140), and Poni S. (ORCID:0000-0002-7238-2613)

Abstract

Maintaining optimal grape acidity at harvest is one of the most complicated challenges under climate change pressures, especially in early ripening cultivars. Warming trends are compressing vine phenology and fostering berry malic acid respiration. In this work, over four years, we evaluated yield components and fruit ripening in two local varieties in the Colli Piacentini, Ortrugo (ORT) and Barbesino (BRB). Our goal was to evaluate their ability to maintain satisfying acidity at harvest and understand the limits and features of the genetic control over organic acid degradation during ripening. The two varieties exhibited comparable yield and grape total soluble solids (TSS) accumulation dynamics, but BRB showed consistently higher acidity during the entire ripening process in any of the four years. BRB's higher acidity was linked to higher malic acid concentrations. ORT had earlier onset of malic acid degradation than BRB and lower maximum malic acid degradation rates. Malic acid degradation rates were lower in ORT also later in the season, until harvest. However, correlations built between malic acid degradation rates and instantaneous malic acid concentration revealed that BRB had a consistently lower malic acid loss for values of malate < 10 g/L. Our work demonstrates that there is a genetic control over the malic acid degradation rates exhibited at varying malic acid concentrations and higher acidity at harvest can be found in varieties exhibiting low malic acid degradation rates when malic acid is < 10 g/L. Post-veraison berry growth rates could interact with genotype effects. The analysis of the correlation can be used at different scales to identify cultivars retaining higher acidity at harvest.

Published
2023

11. A 3-year application of different mycorrhiza-based plant biostimulants distinctively modulates photosynthetic performance, leaf metabolism, and fruit quality in grapes (Vitis vinifera L.)

Author

Ganugi, Paola, Caffi, Tito, Gabrielli, Mario, Secomandi, Elena, Fiorini, Andrea, Zhang, Leilei, Bellotti, Gabriele, Puglisi, Edoardo, Fittipaldi, M. B., Asinari, Florencia, Tabaglio, Vincenzo, Trevisan, Marco, Lucini, Luigi, Ganugi P., Caffi T. (ORCID:0000-0001-9929-4130), Gabrielli M. (ORCID:0000-0002-9313-0140), Secomandi E., Fiorini A. (ORCID:0000-0002-5601-2954), Zhang L. (ORCID:0000-0002-9278-0459), Bellotti G. (ORCID:0000-0001-8521-3256), Puglisi E. (ORCID:0000-0001-5051-0971), Asinari F., Tabaglio V. (ORCID:0000-0003-3456-1589), Trevisan M. (ORCID:0000-0002-4002-9946), Lucini L. (ORCID:0000-0002-5133-9464), Ganugi, Paola, Caffi, Tito, Gabrielli, Mario, Secomandi, Elena, Fiorini, Andrea, Zhang, Leilei, Bellotti, Gabriele, Puglisi, Edoardo, Fittipaldi, M. B., Asinari, Florencia, Tabaglio, Vincenzo, Trevisan, Marco, Lucini, Luigi, Ganugi P., Caffi T. (ORCID:0000-0001-9929-4130), Gabrielli M. (ORCID:0000-0002-9313-0140), Secomandi E., Fiorini A. (ORCID:0000-0002-5601-2954), Zhang L. (ORCID:0000-0002-9278-0459), Bellotti G. (ORCID:0000-0001-8521-3256), Puglisi E. (ORCID:0000-0001-5051-0971), Asinari F., Tabaglio V. (ORCID:0000-0003-3456-1589), Trevisan M. (ORCID:0000-0002-4002-9946), and Lucini L. (ORCID:0000-0002-5133-9464)

Abstract

The use of microbial biostimulants in agriculture is recognized as a sustainable approach to promoting crop productivity and quality due to improved nutrient uptake, enhanced stress tolerance, and improved ability to cope with non-optimal environments. The present paper aimed to comparatively investigate the effect of seven different commercial mycorrhizal-based treatments in terms of yield, phytochemical components, and technological traits of Malvasia di Candia Aromatica grape (Vitis vinifera L.) plants. Metabolomic analysis and photosynthetic performance were first investigated in leaves to point out biochemical differences related to plant growth. Higher photosynthetic efficiency and better PSII functioning were found in biostimulant-treated vines, reflecting an overall decrease in photoinhibition compared to untreated plants. Untargeted metabolomics followed by multivariate statistics highlighted a robust reprogramming of primary (lipids) and secondary (alkaloids and terpenoids) metabolites in treated plants. The analysis of berry yield and chemical components exhibited significant differences depending on the biostimulant product. Generally, berries obtained from treated plants displayed improved contents of polyphenols and sugars, while yield remained unchanged. These results elucidated the significant role of microbial biostimulants in determining the quality of grape berries and eliciting biochemical changes in vines.

Published
2023

12. Reintroducing Autochthonous Minor Grapevine Varieties to Improve Wine Quality and Viticulture Sustainability in a Climate Change Scenario

Author

Frioni, Tommaso, Romanini, Elia, Pagani, Silvia, Del Zozzo, Filippo, Lambri, Milena, Vercesi, Alberto, Gatti, Matteo, Poni, Stefano, Gabrielli, Mario, Frioni, T (ORCID:0000-0002-8663-3022), Romanini, E, Pagani, S, Del Zozzo, F (ORCID:0000-0001-6060-420X), Lambri, M (ORCID:0000-0003-4330-2888), Vercesi, A (ORCID:0000-0003-0845-0500), Gatti, M (ORCID:0000-0003-4195-7709), Poni, S (ORCID:0000-0002-7238-2613), Gabrielli, M (ORCID:0000-0002-9313-0140), Frioni, Tommaso, Romanini, Elia, Pagani, Silvia, Del Zozzo, Filippo, Lambri, Milena, Vercesi, Alberto, Gatti, Matteo, Poni, Stefano, Gabrielli, Mario, Frioni, T (ORCID:0000-0002-8663-3022), Romanini, E, Pagani, S, Del Zozzo, F (ORCID:0000-0001-6060-420X), Lambri, M (ORCID:0000-0003-4330-2888), Vercesi, A (ORCID:0000-0003-0845-0500), Gatti, M (ORCID:0000-0003-4195-7709), Poni, S (ORCID:0000-0002-7238-2613), and Gabrielli, M (ORCID:0000-0002-9313-0140)

Abstract

One of the major challenges that global warming poses to viticulture is the maintenance of adequate acidity at maturity in white grapes for sparkling winemaking.)is issue arises from three main occurrences: (i) with higher temperatures, degradation of malic acid is significantly enhanced; (ii) with a general advancement in grapevine phenology, grape maturity may occur under suboptimal climatic conditions; and (iii) harvesting grapes at "traditional" dates results in overripe fruits for sparkling destinations. In this biennial work, we compared the fruit and must composition of a local, widely grown white grape variety in the Colli Piacentini area (cv. Ortrugo, ORT) with those of a minor autochthonous variety, namely, Barbesino (BRB). Furthermore, we compared the composition, aromatic, and sensory profiles of wines obtained from ORTand BRB grapes picked on the same date and, in addition, of a second Barbesino wine from late harvest (BRB-LH). ORT and BRB had a similar sugar accumulation dynamic, whereas BRB exhibited a delayed loss of titratable acidity. In more details, BRB had lower malic acid degradation rates when malate concentration was <9 g/L. As a result, with comparable yield and total soluble solid content (TSS) (similar to 20 degrees Brix), BRB had a higher berry titratable acidity and malic acid concentration at harvest than ORT. BRB wines showed the highest titratable acidity (TA), while ORT had the lowest TA and a higher pH, and as expected, BRB-LH had the highest pH and a lower TA than BRB although still higher than those of ORTwine.)e aroma profiles of wines were mainly characterized by fermentative aromas, including esters, fatty acids, higher alcohols, and C-6 compounds, and BRB-LH wines showed the highest concentration of higher alcohols, while the fermentative esters were higher in ORTwines. Panelists considered BRB significantly fresher and with bigger aroma intensity than ORT, confirming that the higher acidity detected in BRB musts is well pr

Published
2023

13. Characterization of Durum-Wheat Pasta Containing Resistant Starch from Debranched Waxy Rice Starch

Author

Cervini, Mariasole, Gabrielli, Mario, Spigno, Giorgia, Giuberti, Gianluca, Cervini M., Gabrielli M. (ORCID:0000-0002-9313-0140), Spigno G. (ORCID:0000-0003-1636-6851), Giuberti G. (ORCID:0000-0002-0135-1609), Cervini, Mariasole, Gabrielli, Mario, Spigno, Giorgia, Giuberti, Gianluca, Cervini M., Gabrielli M. (ORCID:0000-0002-9313-0140), Spigno G. (ORCID:0000-0003-1636-6851), and Giuberti G. (ORCID:0000-0002-0135-1609)

Abstract

Durum wheat spaghetti samples prepared with increasing levels of resistant starch (RS) from debranched waxy rice starch (DWRS; i.e., 0, 5, 10, 15 g/100 g w/w) were analyzed for chemical composition, quality and sensory parameters and in vitro starch digestion. All the DWRS-containing spaghetti was “high in fibre”, the dietary fiber content being > 6 g/100 g. In addition, spaghetti with the highest level of DWRS showed the highest RS content (p < 0.05), being 11.4 g/100 g dry matter. The starch hydrolysis index decreased (p < 0.05) as the level of DWRS increased, with a reduction of >20% comparing the 15-DWRS pasta to the control. DWRS had a negative impact on quality parameters, especially at higher DWRS levels. The use of DWRS shortened the optimal cooking time and impacted the samples’ cooking loss, firmness, and stickiness. In addition, sensory analysis revealed differences among samples. However, irrespective of the level of DWRS in the recipe, the score for all attributes was > 5, which is considered the limit of acceptability. Substituting part of the semolina flour with DWRS increased the level of RS and the overall nutritional profile and affected the quality of semolina pasta, mainly at higher levels in the recipe.

Published
2023

14. Teaching suturing skill to emergency physicians: the experience of a hands-on practical training in a laboratory simulation-based setting

Author

Manno, Alberto, Pascale, M. M., Santoro, M. C., Saviano, Angela, Benicchi, Andrea, Gabrielli, Maurizio, Covino, Marcello, Franceschi, Francesco, Manno A., Saviano A. (ORCID:0000-0002-2820-7180), Benicchi A., Gabrielli M., Covino M. (ORCID:0000-0002-6709-2531), Franceschi F. (ORCID:0000-0001-6266-445X), Manno, Alberto, Pascale, M. M., Santoro, M. C., Saviano, Angela, Benicchi, Andrea, Gabrielli, Maurizio, Covino, Marcello, Franceschi, Francesco, Manno A., Saviano A. (ORCID:0000-0002-2820-7180), Benicchi A., Gabrielli M., Covino M. (ORCID:0000-0002-6709-2531), and Franceschi F. (ORCID:0000-0001-6266-445X)

Abstract

Both specialists and trainees in emergency medicine are often unaware of the principles of good suturing. Hands-on training course was proposed to both members of the staff (group A) and trainees (group B) of the emergency department of our hospital. Familiarity with all aspects of the operation, pre-course 0%, postcourse 79% (group A) and 85.71% (group B)-p<0.000); clear economy of movement and maximum efficiency, pre-course 0%, post-course 73.8% (group A) and 89.80% (group B)-p<0.000; fluid moves with instruments and no awkwardness, pre-course 0%, post-course 73.8% (group A) and 89.80% (group B)-p<0.000; obviously planned course of operation with effortless flow from one move to the next, pre-course 0%, post-course 79% (group A) and 89,80% (group B)-p<0.000; strategically used assistants to the best advantage of all time, pre-course 0%, post-course 73,8% (group A) and 89,80% (group B)-p<0.000; improvement in dexterity, 79.5% of students post-course). The course was judged very useful by 94.8% of students. Attending suturing skill courses could be very useful for both trainees and specialists in emergency medicine.

Published
2023

16. Extracellular transglutaminase-2, nude or associated with astrocytic extracellular vesicles, modulates neuronal calcium homeostasis

Author

Tonoli, E, Verduci, I, Gabrielli, M, Prada, I, Forcaia, G, Coveney, C, Pia Savoca, M, Boocock, DJ, Sancini, G, Mazzanti, M, Verderio, C, Verderio, EAM, Tonoli, E, Verduci, I, Gabrielli, M, Prada, I, Forcaia, G, Coveney, C, Savoca, M, Boocock, D, Sancini, G, Mazzanti, M, Verderio, C, and Verderio, E

Subjects
Adenosine Triphosphatases, Neurons, Transglutaminase-2, General Neuroscience, Calcium homeostasi, Sodium-Calcium Exchanger, Extracellular Vesicles, BIO/09 - FISIOLOGIA, Astrocytes, Hippocampal neuron, Homeostasis, Humans, Calcium, Protein Glutamine gamma Glutamyltransferase 2, Extracellular vesicle, Astrocyte
Abstract

We have uncovered a novel role for astrocytes-derived extracellular vesicles (EVs) in controlling intraneuronal Ca2+ concentration ([Ca2+]i) and identified transglutaminase-2 (TG2) as a surface-cargo of astrocytes-derived EVs. Incubation of hippocampal neurons with primed astrocyte-derived EVs have led to an increase in [Ca2+]i, unlike EVs from TG2-knockout astrocytes. Exposure of neurons or brain slices to extracellular TG2 promoted a [Ca2+]i rise, which was reversible upon TG2 removal and was dependent on Ca2+ influx through the plasma membrane. Patch-clamp and calcium imaging recordings revealed TG2-dependent neuronal membrane depolarization and activation of inward currents, due to the Na+/Ca2+-exchanger (NCX) operating in the reverse mode and indirect activation of L-type VOCCs, as indicated by VOCCs/NCX pharmacological inhibitors. A subunit of Na+/K+-ATPase was selected by comparative proteomics and identified as being functionally inhibited by extracellular TG2, implicating Na+/K+-ATPase inhibition in NCX reverse mode-switching leading to Ca2+ influx and higher basal [Ca2+]i. These data suggest that reactive astrocytes control intraneuronal [Ca2+]i through release of EVs with TG2 as responsible cargo, which could have a significant impact on synaptic activity in brain inflammation.

Published
2022
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19. Cognitive reserve as a useful variable to address robotic or conventional upper limb rehabilitation treatment after stroke: a multicentre study of the Fondazione Don Carlo Gnocchi

Author

Padua, L, Imbimbo, I, Aprile, I, Loreti, C, Germanotta, M, Coraci, D, Piccinini, G, Pazzaglia, C, Santilli, C, Cruciani, A, Carrozza, M, Pecchioli, C, Loreti, S, Lattanzi, S, Cortellini, L, Papadopoulou, D, Liberti, G, Panzera, F, Mitrione, P, Ruzzi, D, Rinaldi, G, Insalaco, S, De Santis, F, Spinelli, P, Marsan, S, Bastoni, I, Pellegrino, A, Petitti, T, Montesano, A, Castagna, A, Grosso, C, Ammenti, P, Cattaneo, D, Azzinnaro, L, Barbieri, D, Cassani, S, Corrini, C, Meotti, M, Parelli, R, Spedicato, A, Zocchi, M, Loffi, M, Manenti, D, Negri, L, Gramatica, F, Gower, V, Galeri, S, Noro, F, Medici, L, Garattini, R, Bariselli, F, Luli, M, Ricca, M, Negrini, S, Diverio, M, Giannini, E, Gabrielli, A, Deidda, B, Gnetti, B, Beatini, P, Callegari, S, Cabano, B, Converti, F, Pizzi, A, Falsini, C, Romanelli, A, De Luca, G, Vannetti, F, Simoncini, E, Martini, M, Peccini, E, Cecchi, F, Avila, L, Gabrielli, M, Barilli, M, Bertocchi, E, Giannarelli, G, Lerda, E, Vasoli, M, Rossi, P, Marsili, V, Tognoli, B, Bertolini, A, Vastola, G, Speranza, G, Colella, M, Mosca, R, Competiello, G, Chiusano, A, Della Vecchia, A, Soriano, P, Pagliarulo, M, Remollino, V, Langone, E, Santarsiero, R, Magliulo, M, Araneo, G, Galantucci, L, Lioi, N, Marrazzo, F, Larocca, S, Calia, R, Benevento, S, Toscano, O, Lategana, M, Padua L., Imbimbo I., Aprile I., Loreti C., Germanotta M., Coraci D., Piccinini G., Pazzaglia C., Santilli C., Cruciani A., Carrozza M. C., Pecchioli C., Loreti S., Lattanzi S., Cortellini L., Papadopoulou D., Liberti G., Panzera F., Mitrione P., Ruzzi D., Rinaldi G., Insalaco S., De Santis F., Spinelli P., Marsan S., Bastoni I., Pellegrino A., Petitti T., Montesano A., Castagna A., Grosso C., Ammenti P., Cattaneo D., Azzinnaro L., Barbieri D., Cassani S., Corrini C., Meotti M., Parelli R., Spedicato A., Zocchi M., Loffi M., Manenti D., Negri L., Gramatica F., Gower V., Galeri S., Noro F., Medici L., Garattini R., Bariselli F., Luli M., Ricca M., Negrini S., Diverio M., Giannini E., Gabrielli A., Deidda B., Gnetti B., Beatini P., Callegari S., Cabano B., Converti F., Pizzi A., Falsini C., Romanelli A., De Luca G., Vannetti F., Simoncini E., Martini M., Peccini E., Cecchi F., Avila L., Gabrielli M. A., Barilli M., Bertocchi E., Giannarelli G., Lerda E., Vasoli M., Rossi P., Marsili V., Tognoli B., Bertolini A., Vastola G., Speranza G., Colella M., Mosca R., Competiello G., Chiusano A., Della Vecchia A., Soriano P., Pagliarulo M., Remollino V., Langone E., Santarsiero R., Magliulo M., Araneo G., Galantucci L., Lioi N., Marrazzo F., Larocca S., Calia R., Benevento S., Toscano O., Lategana M., Padua, L, Imbimbo, I, Aprile, I, Loreti, C, Germanotta, M, Coraci, D, Piccinini, G, Pazzaglia, C, Santilli, C, Cruciani, A, Carrozza, M, Pecchioli, C, Loreti, S, Lattanzi, S, Cortellini, L, Papadopoulou, D, Liberti, G, Panzera, F, Mitrione, P, Ruzzi, D, Rinaldi, G, Insalaco, S, De Santis, F, Spinelli, P, Marsan, S, Bastoni, I, Pellegrino, A, Petitti, T, Montesano, A, Castagna, A, Grosso, C, Ammenti, P, Cattaneo, D, Azzinnaro, L, Barbieri, D, Cassani, S, Corrini, C, Meotti, M, Parelli, R, Spedicato, A, Zocchi, M, Loffi, M, Manenti, D, Negri, L, Gramatica, F, Gower, V, Galeri, S, Noro, F, Medici, L, Garattini, R, Bariselli, F, Luli, M, Ricca, M, Negrini, S, Diverio, M, Giannini, E, Gabrielli, A, Deidda, B, Gnetti, B, Beatini, P, Callegari, S, Cabano, B, Converti, F, Pizzi, A, Falsini, C, Romanelli, A, De Luca, G, Vannetti, F, Simoncini, E, Martini, M, Peccini, E, Cecchi, F, Avila, L, Gabrielli, M, Barilli, M, Bertocchi, E, Giannarelli, G, Lerda, E, Vasoli, M, Rossi, P, Marsili, V, Tognoli, B, Bertolini, A, Vastola, G, Speranza, G, Colella, M, Mosca, R, Competiello, G, Chiusano, A, Della Vecchia, A, Soriano, P, Pagliarulo, M, Remollino, V, Langone, E, Santarsiero, R, Magliulo, M, Araneo, G, Galantucci, L, Lioi, N, Marrazzo, F, Larocca, S, Calia, R, Benevento, S, Toscano, O, Lategana, M, Padua L., Imbimbo I., Aprile I., Loreti C., Germanotta M., Coraci D., Piccinini G., Pazzaglia C., Santilli C., Cruciani A., Carrozza M. C., Pecchioli C., Loreti S., Lattanzi S., Cortellini L., Papadopoulou D., Liberti G., Panzera F., Mitrione P., Ruzzi D., Rinaldi G., Insalaco S., De Santis F., Spinelli P., Marsan S., Bastoni I., Pellegrino A., Petitti T., Montesano A., Castagna A., Grosso C., Ammenti P., Cattaneo D., Azzinnaro L., Barbieri D., Cassani S., Corrini C., Meotti M., Parelli R., Spedicato A., Zocchi M., Loffi M., Manenti D., Negri L., Gramatica F., Gower V., Galeri S., Noro F., Medici L., Garattini R., Bariselli F., Luli M., Ricca M., Negrini S., Diverio M., Giannini E., Gabrielli A., Deidda B., Gnetti B., Beatini P., Callegari S., Cabano B., Converti F., Pizzi A., Falsini C., Romanelli A., De Luca G., Vannetti F., Simoncini E., Martini M., Peccini E., Cecchi F., Avila L., Gabrielli M. A., Barilli M., Bertocchi E., Giannarelli G., Lerda E., Vasoli M., Rossi P., Marsili V., Tognoli B., Bertolini A., Vastola G., Speranza G., Colella M., Mosca R., Competiello G., Chiusano A., Della Vecchia A., Soriano P., Pagliarulo M., Remollino V., Langone E., Santarsiero R., Magliulo M., Araneo G., Galantucci L., Lioi N., Marrazzo F., Larocca S., Calia R., Benevento S., Toscano O., and Lategana M.

Abstract

Background and purpose: Rehabilitation plays a central role in stroke recovery. Besides conventional therapy, technological treatments have become available. The effectiveness and appropriateness of technological rehabilitation are not yet well defined; hence, research focused on different variables impacting recovery is needed. Results from the literature identified cognitive reserve (CR) as a variable impacting on the cognitive outcome. In this paper, the aim was to evaluate whether CR influences the motor outcome in patients after stroke treated with conventional or robotic therapy and whether it may influence one treatment rather than another. Methods: Seventy-five stroke patients were enrolled in five Italian neurological rehabilitation centres. Patients were assigned either to a robotic group, rehabilitation by means of robotic devices, or to a conventional group, where a traditional approach was used. Patients were evaluated at baseline and after rehabilitation treatment of 6 weeks through the Action Research Arm Test (ARAT), the Motricity Index (MI) and the Barthel Index (BI). CR was assessed at baseline using the Cognitive Reserve Index (CRI) questionnaire. Results: Considering all patients, a weak correlation was found between the CRI related to leisure time and MI evolution (r = 0.276; P = 0.02). Amongst the patients who performed a robotic rehabilitation, a moderate correlation emerged between the CRI related to working activities and MI evolution (r = 0.422; P = 0.02). Conclusions: Our results suggest that CR may influence the motor outcome. For each patient, CR and its subcategories should be considered in the choice between conventional and robotic treatment.

Published
2020

20. No association between post-exercise high-sensitivity troponin T levels and CAD

Author

Gabrielli, M., Lanza, G. A., Baroni, S., Lamendola, P., Crea, F., Franceschi, F., Gabrielli M., Lanza G. A. (ORCID:0000-0003-2187-6653), Baroni S. (ORCID:0000-0002-3410-2617), Crea F. (ORCID:0000-0001-9404-8846), Franceschi F. (ORCID:0000-0001-6266-445X), Gabrielli, M., Lanza, G. A., Baroni, S., Lamendola, P., Crea, F., Franceschi, F., Gabrielli M., Lanza G. A. (ORCID:0000-0003-2187-6653), Baroni S. (ORCID:0000-0002-3410-2617), Crea F. (ORCID:0000-0001-9404-8846), and Franceschi F. (ORCID:0000-0001-6266-445X)

Abstract

N/A

Published
2020

21. A machine-learning parsimonious multivariable predictive model of mortality risk in patients with Covid-19

Author

Murri, Rita, Lenkowicz, Jacopo, Masciocchi, Carlotta, Iacomini, C., Fantoni, Massimo, Damiani, Andrea, Marchetti, A., Sergi, P. D. A., Arcuri, G., Cesario, Alfredo, Patarnello, S., Antonelli, Massimo, Bellantone, Rocco Domenico Alfonso, Bernabei, Roberto, Boccia, Stefania, Calabresi, Paolo, Cambieri, Andrea, Cauda, Roberto, Colosimo, Cesare, Crea, Filippo, De Maria Marchiano, Ruggero, De Stefano, Valerio, Franceschi, Francesco, Gasbarrini, Antonio, Parolini, Ornella, Richeldi, Luca, Sanguinetti, Maurizio, Urbani, Andrea, Zega, Maurizio, Scambia, Giovanni, Valentini, Vincenzo, Armuzzi, Alessandro, Barba, Marta, Baroni, Silvia, Bellesi, Silvia, Bentivoglio, Anna Rita, Biasucci, Luigi Marzio, Biscetti, Federico, Candelli, Marcello, Capalbo, Gennaro, Cattani, P., Chiusolo, Patrizia, Cingolani, Antonella, Corbo, Giuseppe Maria, Covino, Marcello, Cozzolino, A. M., D'Alfonso, Maria Elena, De Angelis, G., De Pascale, Gennaro, Frisullo, Giovanni, Gabrielli, M., Gambassi, Giovanni, Garcovich, M., Gremese, Elisa, Grieco, D. L., Iaconelli, A., Iorio, Raffaele, Landi, Francesco, Larici, Anna Rita, Liuzzo, Giovanna, Maviglia, Riccardo, Miele, Luca, Montalto, Massimo, Natale, Luigi, Nicolotti, Nicola, Ojetti, Veronica, Pompili, Maurizio, Posteraro, Brunella, Rapaccini, Gian Ludovico, Rinaldi, R., Rossi, E., Santoliquido, Angelo, Sica, Simona, Tamburrini, Enrica, Teofili, Luciana, Testa, A., Tosoni, A., Trani, Carlo, Varone, Francesco, and Verme, L. Z. D.

Subjects
Male, medicine.medical_specialty, Multivariate analysis, Science, Youden's J statistic, Rome, Cross-validation, Article, Cohort Studies, Machine Learning, Models, Internal medicine, medicine, 80 and over, Humans, Hospital Mortality, Pandemics, Aged, Aged, 80 and over, Multidisciplinary, Framingham Risk Score, Models, Statistical, business.industry, SARS-CoV-2, COVID-19, Emergency department, Statistical, Middle Aged, Blood Cell Count, Oxygen, Quartile, Risk factors, ROC Curve, Settore MED/11 - MALATTIE DELL'APPARATO CARDIOVASCOLARE, Multivariate Analysis, Absolute neutrophil count, Medicine, Female, business, Blood Chemical Analysis, Cohort study
Abstract

The COVID-19 pandemic is impressively challenging the healthcare system. Several prognostic models have been validated but few of them are implemented in daily practice. The objective of the study was to validate a machine-learning risk prediction model using easy-to-obtain parameters to help to identify patients with COVID-19 who are at higher risk of death. The training cohort included all patients admitted to Fondazione Policlinico Gemelli with COVID-19 from March 5, 2020, to November 5, 2020. Afterward, the model was tested on all patients admitted to the same hospital with COVID-19 from November 6, 2020, to February 5, 2021. The primary outcome was in-hospital case-fatality risk. The out-of-sample performance of the model was estimated from the training set in terms of Area under the Receiving Operator Curve (AUROC) and classification matrix statistics by averaging the results of fivefold cross validation repeated 3-times and comparing the results with those obtained on the test set. An explanation analysis of the model, based on the SHapley Additive exPlanations (SHAP), is also presented. To assess the subsequent time evolution, the change in paO2/FiO2 (P/F) at 48 h after the baseline measurement was plotted against its baseline value. Among the 921 patients included in the training cohort, 120 died (13%). Variables selected for the model were age, platelet count, SpO2, blood urea nitrogen (BUN), hemoglobin, C-reactive protein, neutrophil count, and sodium. The results of the fivefold cross-validation repeated 3-times gave AUROC of 0.87, and statistics of the classification matrix to the Youden index as follows: sensitivity 0.840, specificity 0.774, negative predictive value 0.971. Then, the model was tested on a new population (n = 1463) in which the case-fatality rate was 22.6%. The test model showed AUROC 0.818, sensitivity 0.813, specificity 0.650, negative predictive value 0.922. Considering the first quartile of the predicted risk score (low-risk score group), the case-fatality rate was 1.6%, 17.8% in the second and third quartile (high-risk score group) and 53.5% in the fourth quartile (very high-risk score group). The three risk score groups showed good discrimination for the P/F value at admission, and a positive correlation was found for the low-risk class to P/F at 48 h after admission (adjusted R-squared = 0.48). We developed a predictive model of death for people with SARS-CoV-2 infection by including only easy-to-obtain variables (abnormal blood count, BUN, C-reactive protein, sodium and lower SpO2). It demonstrated good accuracy and high power of discrimination. The simplicity of the model makes the risk prediction applicable for patients in the Emergency Department, or during hospitalization. Although it is reasonable to assume that the model is also applicable in not-hospitalized persons, only appropriate studies can assess the accuracy of the model also for persons at home.

Published
2021

22. Astrocytic extracellular vesicles modulate neuronal calcium homeostasis via transglutaminase-2

Author

Elisabetta A.M. Verderio, Maria Pia Savoca, Michele Mazzanti, Verduci I, I. Prada, Giulio Sancini, Forcaia G, David J. Boocock, Clare Coveney, Elisa Tonoli, Gabrielli M, and Verderio C

Subjects
Calcium metabolism, biology, Tissue transglutaminase, Chemistry, Protein subunit, Inflammation, Depolarization, Hippocampal formation, Cell biology, Calcium imaging, Extracellular, medicine, biology.protein, medicine.symptom
Abstract

We have uncovered a novel role for astrocytes-derived extracellular vesicles (EVs) in controlling intraneuronal Ca2+concentration ([Ca2+]i) and identified transglutaminase-2 (TG2) as a surface-cargo of astrocytes-derived EVs. Incubation of hippocampal neurons with primed astrocyte-derived EVs have led to an increase in [Ca2+]i, unlike EVs from TG2-knockout astrocytes. Exposure of neurons or brain slices to extracellular TG2 promoted a [Ca2+]irise, which was reversible upon TG2 removal and was dependent on Ca2+influx through the plasma membrane. Patch-clamp and calcium imaging recordings revealed TG2-dependent neuronal membrane depolarisation and activation of inward currents, due to the opening of L-type-VOCCs and to Na+/Ca2+-exchanger (NCX) operation in the reverse mode, as indicated by VOCCs/NCX pharmacological inhibitors. A subunit of Na+/K+-ATPase was selected by comparative proteomics and identified as being functionally inhibited by extracellular TG2, implicating Na+/K+-ATPase inhibition in NCX reverse mode-switching leading to Ca2+influx and higher basal [Ca2+]i. These data suggest that reactive astrocytes control intraneuronal [Ca2+]ithrough release of EVs with TG2 as responsible cargo, which could have a significant impact on synaptic activity in brain inflammation.

Published
2021
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23. Cognitive reserve as a useful variable to address robotic or conventional upper limb rehabilitation treatment after stroke: a multicentre study of the Fondazione Don Carlo Gnocchi

Author

Padua, L., Imbimbo, I., Aprile, I., Loreti, C., Germanotta, M., Coraci, D., Piccinini, G., Pazzaglia, C., Santilli, C., Cruciani, A., Carrozza, M. C., Pecchioli, C., Loreti, S., Lattanzi, S., Cortellini, L., Papadopoulou, D., Liberti, G., Panzera, F., Mitrione, P., Ruzzi, D., Rinaldi, G., Insalaco, S., De Santis, F., Spinelli, P., Marsan, S., Bastoni, I., Pellegrino, A., Petitti, T., Montesano, A., Castagna, A., Grosso, C., Ammenti, P., Cattaneo, D., Azzinnaro, L., Barbieri, D., Cassani, S., Corrini, C., Meotti, M., Parelli, R., Spedicato, A., Zocchi, M., Loffi, M., Manenti, D., Negri, L., Gramatica, F., Gower, V., Galeri, S., Noro, F., Medici, L., Garattini, R., Bariselli, F., Luli, M., Ricca, M., Negrini, S., Diverio, M., Giannini, E., Gabrielli, A., Deidda, B., Gnetti, B., Beatini, P., Callegari, S., Cabano, B., Converti, F., Pizzi, A., Falsini, C., Romanelli, A., De Luca, G., Vannetti, F., Simoncini, E., Martini, M., Peccini, E., Cecchi, F., Avila, L., Gabrielli, M. A., Barilli, M., Bertocchi, E., Giannarelli, G., Lerda, E., Vasoli, M., Rossi, P., Marsili, V., Tognoli, B., Bertolini, A., Vastola, G., Speranza, G., Colella, M., Mosca, R., Competiello, G., Chiusano, A., Della Vecchia, A., Soriano, P., Pagliarulo, M., Remollino, V., Langone, E., Santarsiero, R., Magliulo, M., Araneo, G., Galantucci, L., Lioi, N., Marrazzo, F., Larocca, S., Calia, R., Benevento, S., Toscano, O., and Lategana, M.

Subjects
medicine.medical_specialty, Barthel index, medicine.medical_treatment, Cognitive Reserve Index, rehabilitation, Upper Extremity, 03 medical and health sciences, 0302 clinical medicine, Cognitive Reserve, Robotic Surgical Procedures, medicine, Humans, 030212 general & internal medicine, Stroke, Cognitive reserve, robotics, Rehabilitation, business.industry, personalized medicine, stroke, Stroke Rehabilitation, Cognition, Recovery of Function, medicine.disease, Settore MED/26 - NEUROLOGIA, Treatment Outcome, Neurology, Physical therapy, Neurology (clinical), Stroke recovery, Upper limb rehabilitation, business, 030217 neurology & neurosurgery, Settore MED/34 - MEDICINA FISICA E RIABILITATIVA
Abstract

Background and purpose Rehabilitation plays a central role in stroke recovery. Besides conventional therapy, technological treatments have become available. The effectiveness and appropriateness of technological rehabilitation are not yet well defined; hence, research focused on different variables impacting recovery is needed. Results from the literature identified cognitive reserve (CR) as a variable impacting on the cognitive outcome. In this paper, the aim was to evaluate whether CR influences the motor outcome in patients after stroke treated with conventional or robotic therapy and whether it may influence one treatment rather than another. Methods Seventy-five stroke patients were enrolled in five Italian neurological rehabilitation centres. Patients were assigned either to a robotic group, rehabilitation by means of robotic devices, or to a conventional group, where a traditional approach was used. Patients were evaluated at baseline and after rehabilitation treatment of 6 weeks through the Action Research Arm Test (ARAT), the Motricity Index (MI) and the Barthel Index (BI). CR was assessed at baseline using the Cognitive Reserve Index (CRI) questionnaire. Results Considering all patients, a weak correlation was found between the CRI related to leisure time and MI evolution (r = 0.276; P = 0.02). Amongst the patients who performed a robotic rehabilitation, a moderate correlation emerged between the CRI related to working activities and MI evolution (r = 0.422; P = 0.02). Conclusions Our results suggest that CR may influence the motor outcome. For each patient, CR and its subcategories should be considered in the choice between conventional and robotic treatment.

Published
2019
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24. Relationship Between Arterial-Alveolar Oxygen Gradient, Mortality and Admission to Intensive Care Unit in Severe Covid-19 Related Pneumonia: A Pilot Study

Author

Francesco Franceschi, Gabrielli M, Alessandra Esperide, Luca Santarelli, Federico Valletta, Giancristofaro F, and Michele Santoro

Subjects
medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), business.industry, Oxygen gradient, viruses, Public health, Respiratory disease, virus diseases, General Medicine, medicine.disease, Intensive care unit, Pathophysiology, law.invention, Pneumonia, law, Emergency medicine, medicine, Arterial blood, business
Abstract

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARSCoV- 2) is the causative agent of the respiratory disease later named Coronavirus Disease 2019 (COVID-19), remaining a world public health emergency at present...

Published
2020
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25. Hyperspectral imaging to characterize table grapes

Author

Gabrielli, Mario, Lancon-Verdier, V., Picouet, P., Maury, C., Gabrielli M. (ORCID:0000-0002-9313-0140), Gabrielli, Mario, Lancon-Verdier, V., Picouet, P., Maury, C., and Gabrielli M. (ORCID:0000-0002-9313-0140)

Abstract

Table grape quality is of importance for consumers and thus for producers. Its objective quality is usually determined by destructive methods mainly based on sugar content. This study proposed to evaluate the possibility of hyperspectral imaging to characterize table grapes quality through its sugar (TSS), total flavonoid (TF), and total anthocyanin (TA) contents. Different data pretreatments (WD, SNV, and 1st and 2nd derivative) and different methods were tested to get the best prediction models: PLS with full spectra and then Multiple Linear Regression (MLR) were realized after selecting the optimal wavelengths thanks to the regression coefficients (coefficients) and the Variable Importance in Projection (VIP) scores. All models were good at showing that hyperspectral imaging is a relevant method to predict sugar, total flavonoid, and total anthocyanin contents. The best predictions were obtained from optimal wavelength selection based on coefficients for TSS and from VIPs optimal wavelength windows using SNV pre-treatment for total flavonoid and total anthocyanin content. Thus, good prediction models were proposed in order to characterize grapes while reducing the data sets and limit the data storage to enable an industrial use.

Published
2021

26. Use of grape seeds to reduce haze formation in white wines

Author

Romanini, Elia, Mcrae, J. M., Bilogrevic, E., Colangelo, Donato, Gabrielli, Mario, Lambri, Milena, Romanini E., Colangelo D., Gabrielli M. (ORCID:0000-0002-9313-0140), Lambri M. (ORCID:0000-0003-4330-2888), Romanini, Elia, Mcrae, J. M., Bilogrevic, E., Colangelo, Donato, Gabrielli, Mario, Lambri, Milena, Romanini E., Colangelo D., Gabrielli M. (ORCID:0000-0002-9313-0140), and Lambri M. (ORCID:0000-0003-4330-2888)

Abstract

Grape pathogenesis-related (PR) proteins in white wine can induce haze and hinder the sale of the product. Bentonite is used to remove proteins and “heat-stabilise” wine however it is non-selective and can reduce wine quality. Grape seed powder (GSP) has previously been shown to remove PR proteins and reduce haze formation on a lab scale, however the effect on wine sensory properties was unknown and crucial to the evaluation of GSP as a bentonite alternative. Semillon (SEM) and Sauvignon Blanc (SAB) juices (20L in triplicate) were treated with GSP at two doses, Low (7.5 g/L) and High (15 g/L), prior to fermentation. GSP treatment reduced the concentration of wine PR proteins by up to 57% and 37% for SEM and SAB, respectively, and reduced the amount of haze formed in a heat test by up to 75% and 80%, respectively. Sensory analysis conducted by a trained panel showed that for both wine types the high GSP treatments were rated deeper in colour and higher in bitterness than the bentonite controls, with the low GSP treatment having a similar but less pronounced effect on these attributes. The GSP-treated SAB wine showed greater tropical fruit aroma, and pungency, compared to the bentonite control. Use of GSP can reduce the amount of bentonite needed to stabilize wines and may provide a sustainable and effective alternative to bentonite, notably for textural white wine styles.

Published
2021

27. Self-Referencing Method for Relative Color Intensity Analysis Using Mobile-Phone

Author

Wellington S. Souza, Dalton P. de Santana, Marcos A. S. de Oliveira, Renato E. de Araujo, and Gabrielli M. F. de Oliveira

Subjects
Computer science, business.industry, 010401 analytical chemistry, ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION, Process (computing), Color intensity, Color analysis, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Mobile phone, Calibration, Computer vision, Artificial intelligence, 0210 nano-technology, business, Colorimetry
Abstract

Mobile-phones have been widely explored on colorimetric evaluations. However, their use with different built-in image systems and acquisition configuration, in an environment with non-controlled illumination, limits the establishment of an accurate color analysis. To overcome this restriction, the determination of the absolute color of an object can be overlooked and a relative color value determined. In this work, we establish a new approach for spectroscopic evaluation based on cell-phone imaging, with no previous calibration, determining relative color values. The standalone relative color intensity method is evaluated under the use of four distinct mobile-phones and different illumination conditions. The capability to distinguish different color shades exploring the proposed self-referenced relative color intensity technique is appraised. Moreover, the potential use of the method is demonstrated by evaluating the chemical-adsorption process of Cysteamine molecules on gold nanoparticle surfaces. The proposed self-referenced technique can improve and expand the use of mobile-phones in spectroscopic applications.

Published
2018
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29. Sarilumab use in severe SARS-CoV-2 pneumonia

Author

Gremese, Elisa, Cingolani, Antonella, Bosello, Silvia Laura, Alivernini, Stefano, Tolusso, Barbara, Perniola, S., Landi, Francesco, Pompili, Maurizio, Murri, Rita, Santoliquido, Angelo, Garcovich, M., Sali, Michela, De Pascale, Gennaro, Gabrielli, Maurizio, Biscetti, Federico, Montalto, Massimo, Tosoni, Alessio, Gambassi, Giovanni, Rapaccini, Gian Ludovico, Iaconelli, Amerigo, Zileri Del Verme, L., Petricca, L., Fedele, A. L., Lizzio, Marco Maria, Tamburrini, Enrica, Natalello, Gerlando, Gigante, Laura, Bruno, D., Verardi, Lucrezia, Taddei, Eleonora, Calabrese, Anna Chiara, Lombardi, Francesca, Bernabei, Roberto, Cauda, Roberto, Franceschi, Francesco, Landolfi, Raffaele, Richeldi, Luca, Sanguinetti, Maurizio, Fantoni, Massimo, Antonelli, Massimo, Gasbarrini, Antonio, Gremese E. (ORCID:0000-0002-2248-1058), Cingolani A. (ORCID:0000-0002-3793-2755), Bosello S. L. (ORCID:0000-0002-4837-447X), Alivernini S. (ORCID:0000-0002-7383-4212), Tolusso B. (ORCID:0000-0002-9108-6609), Landi F. (ORCID:0000-0002-3472-1389), Pompili M. (ORCID:0000-0001-6699-7980), Murri R. (ORCID:0000-0003-4263-7854), Santoliquido A. (ORCID:0000-0003-1539-4017), Sali M. (ORCID:0000-0003-3609-2990), De Pascale G. (ORCID:0000-0002-8255-0676), Gabrielli M., Biscetti F. (ORCID:0000-0001-7449-657X), Montalto M. (ORCID:0000-0001-8819-3684), Tosoni A., Gambassi G. (ORCID:0000-0002-7030-9359), Rapaccini G. L. (ORCID:0000-0002-6467-857X), Iaconelli A., Lizzio M. M., Tamburrini E. (ORCID:0000-0003-4930-426X), Natalello G., Gigante L., Verardi L., Taddei E., Calabrese A., Lombardi F. (ORCID:0000-0001-5757-8346), Bernabei R. (ORCID:0000-0002-9197-004X), Cauda R. (ORCID:0000-0002-1498-4229), Franceschi F. (ORCID:0000-0001-6266-445X), Landolfi R. (ORCID:0000-0002-7913-8576), Richeldi L. (ORCID:0000-0001-8594-1448), Sanguinetti M. (ORCID:0000-0002-9780-7059), Fantoni M. (ORCID:0000-0001-6913-8460), Antonelli M. (ORCID:0000-0003-3007-1670), Gasbarrini A. (ORCID:0000-0002-7278-4823), Gremese, Elisa, Cingolani, Antonella, Bosello, Silvia Laura, Alivernini, Stefano, Tolusso, Barbara, Perniola, S., Landi, Francesco, Pompili, Maurizio, Murri, Rita, Santoliquido, Angelo, Garcovich, M., Sali, Michela, De Pascale, Gennaro, Gabrielli, Maurizio, Biscetti, Federico, Montalto, Massimo, Tosoni, Alessio, Gambassi, Giovanni, Rapaccini, Gian Ludovico, Iaconelli, Amerigo, Zileri Del Verme, L., Petricca, L., Fedele, A. L., Lizzio, Marco Maria, Tamburrini, Enrica, Natalello, Gerlando, Gigante, Laura, Bruno, D., Verardi, Lucrezia, Taddei, Eleonora, Calabrese, Anna Chiara, Lombardi, Francesca, Bernabei, Roberto, Cauda, Roberto, Franceschi, Francesco, Landolfi, Raffaele, Richeldi, Luca, Sanguinetti, Maurizio, Fantoni, Massimo, Antonelli, Massimo, Gasbarrini, Antonio, Gremese E. (ORCID:0000-0002-2248-1058), Cingolani A. (ORCID:0000-0002-3793-2755), Bosello S. L. (ORCID:0000-0002-4837-447X), Alivernini S. (ORCID:0000-0002-7383-4212), Tolusso B. (ORCID:0000-0002-9108-6609), Landi F. (ORCID:0000-0002-3472-1389), Pompili M. (ORCID:0000-0001-6699-7980), Murri R. (ORCID:0000-0003-4263-7854), Santoliquido A. (ORCID:0000-0003-1539-4017), Sali M. (ORCID:0000-0003-3609-2990), De Pascale G. (ORCID:0000-0002-8255-0676), Gabrielli M., Biscetti F. (ORCID:0000-0001-7449-657X), Montalto M. (ORCID:0000-0001-8819-3684), Tosoni A., Gambassi G. (ORCID:0000-0002-7030-9359), Rapaccini G. L. (ORCID:0000-0002-6467-857X), Iaconelli A., Lizzio M. M., Tamburrini E. (ORCID:0000-0003-4930-426X), Natalello G., Gigante L., Verardi L., Taddei E., Calabrese A., Lombardi F. (ORCID:0000-0001-5757-8346), Bernabei R. (ORCID:0000-0002-9197-004X), Cauda R. (ORCID:0000-0002-1498-4229), Franceschi F. (ORCID:0000-0001-6266-445X), Landolfi R. (ORCID:0000-0002-7913-8576), Richeldi L. (ORCID:0000-0001-8594-1448), Sanguinetti M. (ORCID:0000-0002-9780-7059), Fantoni M. (ORCID:0000-0001-6913-8460), Antonelli M. (ORCID:0000-0003-3007-1670), and Gasbarrini A. (ORCID:0000-0002-7278-4823)

Abstract

Background: Interleukin-6 signal blockade showed preliminary beneficial effects in treating inflammatory response against SARS-CoV-2 leading to severe respiratory distress. Herein we describe the outcomes of off-label intravenous use of Sarilumab in severe SARS-CoV-2-related pneumonia. Methods: 53 patients with SARS-CoV-2 severe pneumonia received intravenous Sarilumab; pulmonary function improvement or Intensive Care Unit (ICU) admission rate in medical wards, live discharge rate in ICU treated patients and safety profile were recorded. Sarilumab 400 mg was administered intravenously on day 1, with eventual additional infusion based on clinical judgement, and patients were followed for at least 14 days, unless previously discharged or dead. Findings: Of the 53 SARS-CoV-2pos patients receiving Sarilumab, 39(73·6%) were treated in medical wards [66·7% with a single infusion; median PaO2/FiO2:146(IQR:120–212)] while 14(26·4%) in ICU [92·6% with a second infusion; median PaO2/FiO2: 112(IQR:100–141.5)]. Within the medical wards, 7(17·9%) required ICU admission, 4 of whom were re-admitted to the ward within 5–8 days. At 19 days median follow-up, 89·7% of medical inpatients significantly improved (46·1% after 24 h, 61·5% after 3 days), 70·6% were discharged from the hospital and 85·7% no longer needed oxygen therapy. Within patients receiving Sarilumab in ICU, 64·2% were discharged from ICU to the ward and 35·8% were still alive at the last follow-up. Overall mortality rate was 5·7%. Interpretation: IL-6R inhibition appears to be a potential treatment strategy for severe SARS-CoV-2 pneumonia and intravenous Sarilumab seems a promising treatment approach showing, in the short term, an important clinical outcome and good safety.

Published
2020

30. Chloroanisoles occurrence in wine from grapes subjected to electrolyzed water treatments in the vineyard

Author

Gabrielli, Mario, Englezos, V., Rolle, L., Rio Segade, S., Giacosa, S., Cocolin, L., Paissoni, M. A., Lambri, Milena, Rantsiou, K., Maury, C., Gabrielli M. (ORCID:0000-0002-9313-0140), Lambri M. (ORCID:0000-0003-4330-2888), Gabrielli, Mario, Englezos, V., Rolle, L., Rio Segade, S., Giacosa, S., Cocolin, L., Paissoni, M. A., Lambri, Milena, Rantsiou, K., Maury, C., Gabrielli M. (ORCID:0000-0002-9313-0140), and Lambri M. (ORCID:0000-0003-4330-2888)

Abstract

Recently the use of electrolyzed water (EW) attracted much attention as a high-performance, new technology for its potential use in the food industry. The aim of this work was to investigate the impact of grape EW treatments, applied at different time intervals prior to harvest, on the indigenous yeast populations of grape surface (Chenin blanc and Cabernet franc) and the occurrence of 2,4,6-trichloroanisole (TCA) in Cabernet franc wine. In addition, the evolution of inoculated and spontaneous fermentations on treated and non-treated grapes was also considered. The yeast population present on grape berries surface was influenced in a grape variety and EW treatment time-dependent way, since only Chenin blanc grapes treated with EW 7 days prior to harvest had significantly lower yeast population levels, compared to the respective control. Concerning the yeast diversity in the grape samples, a dominance of Aureobasidium pullulans was observed in treated grapes, independently of the grape variety. At the end of alcoholic fermentation, 2,4,6-trichloroanisole was detected in wine when the EW solution was applied at one or two weeks before harvest time. After wine storage, 2,4,6-trichloroanisole and chlorophenols contents generally exhibited a loss relative to initial values. The results showed that EW treatments tended to slightly increase the TCA concentration in final wine and did not affect the fermentation performances and chromatic properties of resulting wine. On the other hand, absorption or desorption phenomena by wine lees could be involved in the change of 2,4,6-trichloroanisole concentration in wine during storage time.

Published
2020

31. Assessment of neurological manifestations in hospitalized patients with COVID-19

Author

Luigetti, Marco, Iorio, Raffaele, Bentivoglio, Anna Rita, Tricoli, Luca, Riso, Vittorio, Marotta, Jessica, Piano, Carla, Primiano, Guido Alessandro, Zileri Del Verme, L., Lo Monaco, Maria Rita, Calabresi, Paolo, Abbate, V., Acampora, N., Addolorato, G., Agostini, F., Ainora, M. E., Akacha, K., Amato, E., Andreani, F., Andriollo, G., Annetta, Maria Giuseppina, Annicchiarico, B. E., Antonelli, Massimo, Antonucci, G., Anzellotti, G. M., Armuzzi, A., Baldi, F., Barattucci, I., Barillaro, C., Barone, F., Bellantone, R. D. A., Bellieni, A., Bello, G., Benicchi, A., Benvenuto, F., Berardini, L., Berloco, F., Bernabei, R., Bianchi, A., Biasucci, D. G., Biasucci, L. M., Bibbo, S., Bini, A., Bisanti, A., Biscetti, F., Bocci, M. G., Bonadia, N., Bongiovanni, F., Borghetti, A., Bosco, G., Bosello, Silvia Laura, Bove, V., Bramato, G., Brandi, V., Bruni, T., Bruno, C., Bruno, D., Bungaro, M. C., Buonomo, A., Burzo, L., Calabrese, A., Calvello, M. R., Cambieri, A., Cambise, C., Camma, G., Candelli, M., Canistro, G., Cantanale, A., Capalbo, G., Capaldi, L., Capone, E., Capristo, E., Carbone, L., Cardone, S., Carelli, S., Carfi, A., Carnicelli, A., Caruso, C., Casciaro, F. A., Catalano, L., Cauda, R., Cecchini, A. L., Cerrito, L., Cesarano, M., Chiarito, A., Cianci, Rossella, Cicchinelli, S., Ciccullo, A., Cicetti, M., Ciciarello, F., Cingolani, A., Cipriani, M. C., Consalvo, M. L., Coppola, G., Corbo, G. M., Corsello, A., Costante, F., Costanzi, M., Covino, M., Crupi, D., Cutuli, S. L., D'Addio, S., D'Alessandro, A., D'Alfonso, M. E., D'Angelo, E., D'Aversa, F., Damiano, F., De Berardinis, G. M., De Cunzo, T., De Gaetano, D. K., De Luca, G., De Matteis, G., De Pascale, G., De Santis, P., De Siena, M., De Vito, F., Del Gatto, V., Del Giacomo, P., Del Zompo, F., Dell'Anna, A. M., Della, P. D., Di Gialleonardo, L., Di Giambenedetto, S., Di Luca, R., Di Maurizio, L., Di Muro, M., Dusina, A., Eleuteri, D., Esperide, A., Fachechi, D., Faliero, D., Falsiroli, C., Fantoni, M., Fedele, A., Feliciani, D., Ferrante, C., Ferrone, G., Festa, R., Fiore, M. C., Flex, A., Forte, E., Franceschi, Francesco, Francesconi, A., Franza, L., Funaro, B., Fuorlo, M., Fusco, D., Gabrielli, M., Gaetani, E., Galletta, C., Gallo, A., Gambassi, G., Garcovich, M., Gasbarrini, A., Gasparrini, I., Gelli, S., Giampietro, A., Gigante, L., Giuliano, G., Giupponi, B., Gremese, E., Grieco, Domenico Luca, Guerrera, M., Guglielmi, V., Guidone, C., Gulli, A., Iaconelli, A., Iafrati, A., Ianiro, Gianluca, Iaquinta, A., Impagnatiello, M., Inchingolo, R., Intini, E., Iorio, R., Izzi, I. M., Jovanovic, T., Kadhim, C., La Macchia, R., La Milia, D. I., Landi, F., Landi, G., Landi, R., Landolfi, R., Leo, M., Leone, P. M., Levantesi, L., Liguori, A., Liperoti, R., Lizzio, M. M., Lo Monaco Maria, R., Locantore, P., Lombardi, F., Lombardi, G., Lopetuso, L., Loria, V., Losito, A. R., Lucia, M. B. P., Macagno, F., Macerola, N., Maggi, G., Maiuro, G., Mancarella, F., Mangiola, F., Manno, A., Marchesini, D., Maresca, G. M., Marrone, G., Martis, I., Martone, A. M., Marzetti, Emanuele, Mattana, C., Matteo, M. V., Maviglia, R., Mazzarella, A., Memoli, C., Miele, Luca, Migneco, A., Mignini, I., Milani, A., Milardi, D., Montalto, M., Montemurro, G., Monti, F., Montini, Luca, Morena, T. C., Morra, V., Morretta, C., Moschese, D., Murace, C. A., Murdolo, M., Murri, Rita, Napoli, M., Nardella, E., Natalello, G., Natalini, D., Navarra, S. M., Nesci, A., Nicoletti, A., Nicoletti, R., Nicoletti, T. F., Nicolo, R., Nicolotti, N., Nista, E. C., Nuzzo, E., Oggiano, M., Ojetti, V., Pagano, F. C., Paiano, G., Pais, C., Pallavicini, F., Palombo, A., Paolillo, F., Papa, Alfredo, Papanice, D., Papparella, L. G., Paratore, M., Parrinello, G., Pasciuto, G., Pasculli, P., Pecorini, G., Perniola, S., Pero, E., Petricca, L., Petrucci, M., Picarelli, C., Piccioni, A., Piccolo, A., Piervincenzi, E., Pignataro, G., Pignataro, R., Pintaudi, G., Pisapia, L., Pizzoferrato, M., Pizzolante, F., Pola, R., Policola, C., Pompili, M., Pontecorvi, F., Pontecorvi, V., Ponziani, F., Popolla, V., Porceddu, E., Porfidia, A., Porro, L. M., Potenza, A., Pozzana, F., Privitera, G., Pugliese, D., Pulcini, G., Racco, S., Raffaelli, F., Ramunno, V., Rapaccini, G. L., Richeldi, Luca, Rinninella, Emanuele, Rocchi, S., Romano, B., Romano, S., Rosa, F., Rossi, L., Rossi, R., Rossini, E., Rota, E., Rovedi, F., Rubino, C., Rumi, G., Russo, A., Sabia, L., Salerno, A., Salini, S., Salvatore, L., Samori, D., Sandroni, Claudio, Sanguinetti, M., Santarelli, L., Santini, P., Santolamazza, D., Santoliquido, A., Santopaolo, F., Santoro, M. C., Sardeo, F., Sarnari, C., Saviano, A., Saviano, L., Scaldaferri, Franco, Scarascia, R., Schepis, T., Schiavello, F., Scoppettuolo, G., Sedda, D., Sessa, F., Sestito, L., Settanni, C., Siciliano, M., Siciliano, V., Sicuranza, R., Simeoni, B., Simonetti, J., Smargiassi, A., Soave, P. M., Sonnino, C., Staiti, D., Stella, C., Stella, L., Stival, E., Taddei, E., Talerico, R., Tamburello, E., Tamburrini, E., Tanzarella, E. S., Tarascio, E., Tarli, C., Tersali, A., Tilli, P., Timpano, J., Torelli, E., Torrini, F., Tosato, M., Tosoni, A., Tricoli, L., Tritto, M., Tumbarello, M., Tummolo, A. M., Vallecoccia, M. S., Valletta, F., Varone, F., Vassalli, F., Ventura, G., Verardi, L., Vetrone, L., Vetrugno, G., Visconti, E., Visconti, F., Viviani, A., Zaccaria, R., Zaccone, C., Zelano, L., Zileri Dal Verme, L., Zuccala, G., Luigetti M. (ORCID:0000-0001-7539-505X), Iorio R. (ORCID:0000-0002-6270-0956), Bentivoglio A. R. (ORCID:0000-0002-9663-095X), Tricoli L., Riso V., Marotta J., Piano C., Primiano G., Lo Monaco M. R. (ORCID:0000-0002-1457-7981), Calabresi P. (ORCID:0000-0003-0326-5509), Annetta M. G. (ORCID:0000-0001-7574-1311), Antonelli M. (ORCID:0000-0003-3007-1670), Bosello S. (ORCID:0000-0002-4837-447X), Cianci R. (ORCID:0000-0001-5378-8442), Franceschi F. (ORCID:0000-0001-6266-445X), Grieco D. L. (ORCID:0000-0002-4557-6308), Ianiro G. (ORCID:0000-0002-8318-0515), Marzetti E. (ORCID:0000-0001-9567-6983), Miele L. (ORCID:0000-0003-3464-0068), Montini L. (ORCID:0000-0003-4602-5134), Murri R. (ORCID:0000-0003-4263-7854), Papa A. (ORCID:0000-0002-4186-7298), Richeldi L. (ORCID:0000-0001-8594-1448), Rinninella E. (ORCID:0000-0002-9165-2367), Sandroni C. (ORCID:0000-0002-8878-2611), Scaldaferri F. (ORCID:0000-0001-8334-7541), Luigetti, Marco, Iorio, Raffaele, Bentivoglio, Anna Rita, Tricoli, Luca, Riso, Vittorio, Marotta, Jessica, Piano, Carla, Primiano, Guido Alessandro, Zileri Del Verme, L., Lo Monaco, Maria Rita, Calabresi, Paolo, Abbate, V., Acampora, N., Addolorato, G., Agostini, F., Ainora, M. E., Akacha, K., Amato, E., Andreani, F., Andriollo, G., Annetta, Maria Giuseppina, Annicchiarico, B. E., Antonelli, Massimo, Antonucci, G., Anzellotti, G. M., Armuzzi, A., Baldi, F., Barattucci, I., Barillaro, C., Barone, F., Bellantone, R. D. A., Bellieni, A., Bello, G., Benicchi, A., Benvenuto, F., Berardini, L., Berloco, F., Bernabei, R., Bianchi, A., Biasucci, D. G., Biasucci, L. M., Bibbo, S., Bini, A., Bisanti, A., Biscetti, F., Bocci, M. G., Bonadia, N., Bongiovanni, F., Borghetti, A., Bosco, G., Bosello, Silvia Laura, Bove, V., Bramato, G., Brandi, V., Bruni, T., Bruno, C., Bruno, D., Bungaro, M. C., Buonomo, A., Burzo, L., Calabrese, A., Calvello, M. R., Cambieri, A., Cambise, C., Camma, G., Candelli, M., Canistro, G., Cantanale, A., Capalbo, G., Capaldi, L., Capone, E., Capristo, E., Carbone, L., Cardone, S., Carelli, S., Carfi, A., Carnicelli, A., Caruso, C., Casciaro, F. A., Catalano, L., Cauda, R., Cecchini, A. L., Cerrito, L., Cesarano, M., Chiarito, A., Cianci, Rossella, Cicchinelli, S., Ciccullo, A., Cicetti, M., Ciciarello, F., Cingolani, A., Cipriani, M. C., Consalvo, M. L., Coppola, G., Corbo, G. M., Corsello, A., Costante, F., Costanzi, M., Covino, M., Crupi, D., Cutuli, S. L., D'Addio, S., D'Alessandro, A., D'Alfonso, M. E., D'Angelo, E., D'Aversa, F., Damiano, F., De Berardinis, G. M., De Cunzo, T., De Gaetano, D. K., De Luca, G., De Matteis, G., De Pascale, G., De Santis, P., De Siena, M., De Vito, F., Del Gatto, V., Del Giacomo, P., Del Zompo, F., Dell'Anna, A. M., Della, P. D., Di Gialleonardo, L., Di Giambenedetto, S., Di Luca, R., Di Maurizio, L., Di Muro, M., Dusina, A., Eleuteri, D., Esperide, A., Fachechi, D., Faliero, D., Falsiroli, C., Fantoni, M., Fedele, A., Feliciani, D., Ferrante, C., Ferrone, G., Festa, R., Fiore, M. C., Flex, A., Forte, E., Franceschi, Francesco, Francesconi, A., Franza, L., Funaro, B., Fuorlo, M., Fusco, D., Gabrielli, M., Gaetani, E., Galletta, C., Gallo, A., Gambassi, G., Garcovich, M., Gasbarrini, A., Gasparrini, I., Gelli, S., Giampietro, A., Gigante, L., Giuliano, G., Giupponi, B., Gremese, E., Grieco, Domenico Luca, Guerrera, M., Guglielmi, V., Guidone, C., Gulli, A., Iaconelli, A., Iafrati, A., Ianiro, Gianluca, Iaquinta, A., Impagnatiello, M., Inchingolo, R., Intini, E., Iorio, R., Izzi, I. M., Jovanovic, T., Kadhim, C., La Macchia, R., La Milia, D. I., Landi, F., Landi, G., Landi, R., Landolfi, R., Leo, M., Leone, P. M., Levantesi, L., Liguori, A., Liperoti, R., Lizzio, M. M., Lo Monaco Maria, R., Locantore, P., Lombardi, F., Lombardi, G., Lopetuso, L., Loria, V., Losito, A. R., Lucia, M. B. P., Macagno, F., Macerola, N., Maggi, G., Maiuro, G., Mancarella, F., Mangiola, F., Manno, A., Marchesini, D., Maresca, G. M., Marrone, G., Martis, I., Martone, A. M., Marzetti, Emanuele, Mattana, C., Matteo, M. V., Maviglia, R., Mazzarella, A., Memoli, C., Miele, Luca, Migneco, A., Mignini, I., Milani, A., Milardi, D., Montalto, M., Montemurro, G., Monti, F., Montini, Luca, Morena, T. C., Morra, V., Morretta, C., Moschese, D., Murace, C. A., Murdolo, M., Murri, Rita, Napoli, M., Nardella, E., Natalello, G., Natalini, D., Navarra, S. M., Nesci, A., Nicoletti, A., Nicoletti, R., Nicoletti, T. F., Nicolo, R., Nicolotti, N., Nista, E. C., Nuzzo, E., Oggiano, M., Ojetti, V., Pagano, F. C., Paiano, G., Pais, C., Pallavicini, F., Palombo, A., Paolillo, F., Papa, Alfredo, Papanice, D., Papparella, L. G., Paratore, M., Parrinello, G., Pasciuto, G., Pasculli, P., Pecorini, G., Perniola, S., Pero, E., Petricca, L., Petrucci, M., Picarelli, C., Piccioni, A., Piccolo, A., Piervincenzi, E., Pignataro, G., Pignataro, R., Pintaudi, G., Pisapia, L., Pizzoferrato, M., Pizzolante, F., Pola, R., Policola, C., Pompili, M., Pontecorvi, F., Pontecorvi, V., Ponziani, F., Popolla, V., Porceddu, E., Porfidia, A., Porro, L. M., Potenza, A., Pozzana, F., Privitera, G., Pugliese, D., Pulcini, G., Racco, S., Raffaelli, F., Ramunno, V., Rapaccini, G. L., Richeldi, Luca, Rinninella, Emanuele, Rocchi, S., Romano, B., Romano, S., Rosa, F., Rossi, L., Rossi, R., Rossini, E., Rota, E., Rovedi, F., Rubino, C., Rumi, G., Russo, A., Sabia, L., Salerno, A., Salini, S., Salvatore, L., Samori, D., Sandroni, Claudio, Sanguinetti, M., Santarelli, L., Santini, P., Santolamazza, D., Santoliquido, A., Santopaolo, F., Santoro, M. C., Sardeo, F., Sarnari, C., Saviano, A., Saviano, L., Scaldaferri, Franco, Scarascia, R., Schepis, T., Schiavello, F., Scoppettuolo, G., Sedda, D., Sessa, F., Sestito, L., Settanni, C., Siciliano, M., Siciliano, V., Sicuranza, R., Simeoni, B., Simonetti, J., Smargiassi, A., Soave, P. M., Sonnino, C., Staiti, D., Stella, C., Stella, L., Stival, E., Taddei, E., Talerico, R., Tamburello, E., Tamburrini, E., Tanzarella, E. S., Tarascio, E., Tarli, C., Tersali, A., Tilli, P., Timpano, J., Torelli, E., Torrini, F., Tosato, M., Tosoni, A., Tricoli, L., Tritto, M., Tumbarello, M., Tummolo, A. M., Vallecoccia, M. S., Valletta, F., Varone, F., Vassalli, F., Ventura, G., Verardi, L., Vetrone, L., Vetrugno, G., Visconti, E., Visconti, F., Viviani, A., Zaccaria, R., Zaccone, C., Zelano, L., Zileri Dal Verme, L., Zuccala, G., Luigetti M. (ORCID:0000-0001-7539-505X), Iorio R. (ORCID:0000-0002-6270-0956), Bentivoglio A. R. (ORCID:0000-0002-9663-095X), Tricoli L., Riso V., Marotta J., Piano C., Primiano G., Lo Monaco M. R. (ORCID:0000-0002-1457-7981), Calabresi P. (ORCID:0000-0003-0326-5509), Annetta M. G. (ORCID:0000-0001-7574-1311), Antonelli M. (ORCID:0000-0003-3007-1670), Bosello S. (ORCID:0000-0002-4837-447X), Cianci R. (ORCID:0000-0001-5378-8442), Franceschi F. (ORCID:0000-0001-6266-445X), Grieco D. L. (ORCID:0000-0002-4557-6308), Ianiro G. (ORCID:0000-0002-8318-0515), Marzetti E. (ORCID:0000-0001-9567-6983), Miele L. (ORCID:0000-0003-3464-0068), Montini L. (ORCID:0000-0003-4602-5134), Murri R. (ORCID:0000-0003-4263-7854), Papa A. (ORCID:0000-0002-4186-7298), Richeldi L. (ORCID:0000-0001-8594-1448), Rinninella E. (ORCID:0000-0002-9165-2367), Sandroni C. (ORCID:0000-0002-8878-2611), and Scaldaferri F. (ORCID:0000-0001-8334-7541)

Abstract

Background and purpose: The objective of this study was to assess the neurological manifestations in a series of consecutive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-positive patients, comparing their frequency with a population hospitalized in the same period for flu/respiratory symptoms, finally not related to SARS-CoV-2. Methods: Patients with flu/respiratory symptoms admitted to Fondazione Policlinico Gemelli hospital from 14 March 2020 to 20 April 2020 were retrospectively enrolled. The frequency of neurological manifestations of patients with SARS-CoV-2 infection was compared with a control group. Results: In all, 213 patients were found to be positive for SARS-CoV-2, after reverse transcriptase polymerase chain reaction on nasal or throat swabs, whilst 218 patients were found to be negative and were used as a control group. Regarding central nervous system manifestations, in SARS-CoV-2-positive patients a higher frequency of headache, hyposmia and encephalopathy always related to systemic conditions (fever or hypoxia) was observed. Furthermore, muscular involvement was more frequent in SARS-CoV-2 infection. Conclusions: Patients with COVID-19 commonly have neurological manifestations but only hyposmia and muscle involvement seem more frequent compared with other flu diseases.

Published
2020

32. Valorizzazione di vini rossi romagnoli attraverso l’impiego di alcuni chiarificanti di origine vegetale: nuovi stili enologici per il mercato dei vini vegan.

Author

Chiusano, L, Lang Adriasola, S., Romanini, Elia, Colangelo, Donato, Gabrielli, Mario, Lambri, Milena, Romanini E., Colangelo D., Gabrielli M. (ORCID:0000-0002-9313-0140), Lambri M. (ORCID:0000-0003-4330-2888), Chiusano, L, Lang Adriasola, S., Romanini, Elia, Colangelo, Donato, Gabrielli, Mario, Lambri, Milena, Romanini E., Colangelo D., Gabrielli M. (ORCID:0000-0002-9313-0140), and Lambri M. (ORCID:0000-0003-4330-2888)

Abstract

La qualità del vino oggi si riflette specialmente nella sua stabilità che ne preserva funzione e apprezzamento dalla messa in bottiglia sino al consumatore finale. La chiarifica è una delle fasi di vinificazione che consente di perseguire tale stabilità. Nel caso dei vini rossi, tale pratica enologica comporta l’aggiunta al vino di proteine aventi l’obiettivo di ridurre la percezione di amaro e di modulare finemente quella dell’astringenza minimizzando secondari effetti sul colore e sulle percezioni aromatiche positive. In alternativa a quelle di origine animale, oggi il mercato propone l’utilizzo di proteine vegetali la cui diffusione è anche sottesa a motivazioni di sicurezza alimentare, naturalità, tendenza di consumo di prodotti vegani. La ricerca, stimolata da finanziamenti di carattere regionale, volti ad aumentare la produttività delle aziende attraverso ricadute positive dei risultati su tutta la filiera, propone studi su scala reale che misurano gli effetti di tali coadiuvanti su vini di ampia diffusione e consumo.

Published
2020

40. Underestimated awakening face-edema caused by a mediastinal syndrome

Author

A Capacci, Di Maurizio L, Emanuele Gilardi, Chiara Cordischi, M Petrucci, Merra G, Franceschi F, Gabrielli M, and Ferrigno F

Subjects
FACE EDEMA, medicine.medical_specialty, business.industry, food and beverages, medicine.disease, Rash, Lymphoma, medicine.anatomical_structure, medicine, Radiology, medicine.symptom, Facial edema, business, Vein
Abstract

The "mediastinal syndrome" is an oncological urgency but, in some situations as in the case report described below, it can present itself in a subtle and inextricable way with symptoms and signs that, if singularly evaluated, can be nonspecific. In particular, we report the case of facial edema and a rash that, developing in an unusual and progressive way during about 2 months, concealed a voluminous mediastinal formation due to the localization of non-Hodgkin B-cell lymphoma that caused a "superior caval vein syndrome".

Published
2019
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43. H. PYLORI GASTRIC INFECTION IN RHEUMATIC DISEASES

Author

Danese, S., Gasbarrini, A., Zoli, A., Candelli, M., Franceschi, F., Ojetti, V., Armuzzi, A., Cammarota, G., Carloni, E., Cremonini, F., Gabrielli, M., Torre, E. Sanz, Zocca, M. A., Canducci, F., Magaro, M., and Gasbarrini, G.

Published
1999

46. Postcardiotomy venoarterial extracorporeal membrane oxygenation in patients aged 70 years or older

Author

Biancari, F. (Fausto), Saeed, D. (Diyar), Fiore, A. (Antonio), Dalén, M. (Magnus), Ruggieri, V. G. (Vito G.), Jónsson, K. (Kristján), Gatti, G. (Giuseppe), Zipfel, S. (Svante), Dell’Aquila, A. M. (Angelo M.), Chocron, S. (Sidney), Bounader, K. (Karl), Amr, G. (Gilles), Settembre, N. (Nicla), Pälve, K. (Kristiina), Loforte, A. (Antonio), Gabrielli, M. (Marco), Livi, U. (Ugolino), Lechiancole, A. (Andrea), Pol, M. (Marek), Netuka, I. (Ivan), Spadaccio, C. (Cristiano), Pettinari, M. (Matteo), De Keyzer, D. (Dieter), Reichart, D. (Daniel), Ragnarsson, S. (Sigurdur), Alkhamees, K. (Khalid), Lichtenberg, A. (Artur), Fux, T. (Thomas), El Dean, Z. (Zein), Fiorentino, M. (Mariafrancesca), Mariscalco, G. (Giovanni), Jeppsson, A. (Anders), Welp, H. (Henryk), Perrotti, A. (Andrea), Biancari, F. (Fausto), Saeed, D. (Diyar), Fiore, A. (Antonio), Dalén, M. (Magnus), Ruggieri, V. G. (Vito G.), Jónsson, K. (Kristján), Gatti, G. (Giuseppe), Zipfel, S. (Svante), Dell’Aquila, A. M. (Angelo M.), Chocron, S. (Sidney), Bounader, K. (Karl), Amr, G. (Gilles), Settembre, N. (Nicla), Pälve, K. (Kristiina), Loforte, A. (Antonio), Gabrielli, M. (Marco), Livi, U. (Ugolino), Lechiancole, A. (Andrea), Pol, M. (Marek), Netuka, I. (Ivan), Spadaccio, C. (Cristiano), Pettinari, M. (Matteo), De Keyzer, D. (Dieter), Reichart, D. (Daniel), Ragnarsson, S. (Sigurdur), Alkhamees, K. (Khalid), Lichtenberg, A. (Artur), Fux, T. (Thomas), El Dean, Z. (Zein), Fiorentino, M. (Mariafrancesca), Mariscalco, G. (Giovanni), Jeppsson, A. (Anders), Welp, H. (Henryk), and Perrotti, A. (Andrea)

Abstract

Background: There is uncertainty whether venoarterial extracorporeal membrane oxygenation (VA-ECMO) should be used in older patients with cardiopulmonary failure after cardiac surgery. Methods: This was a retrospective multicenter study of 781 patients who required postcardiotomy VA-ECMO for cardiopulmonary failure after adult cardiac surgery from 2010 to 2018 at 19 cardiac surgery centers. A parallel systematic review with meta-analysis of the literature was performed. Results: The hospital mortality in the overall Postcardiotomy Venoarterial Extracorporeal Membrane Oxygenation (PC-ECMO) series was 64.4%. A total of 255 patients were 70 years old or older (32.7%), and their hospital mortality was significantly higher than in younger patients (76.1% vs 58.7%; adjusted odds ratio, 2.199; 95% confidence interval [CI], 1.536 to 3.149). Arterial lactate level greater than 6 mmol/L before starting VA-ECMO was the only predictor of hospital mortality among patients 70 years old or older in univariate analysis (82.6% vs 70.4%; P = 0.029). Meta-analysis of current and previous studies showed that early mortality after postcardiotomy VA-ECMO was significantly higher in patients aged 70 years or older compared with younger patients (odds ratio, 2.09; 95% CI, 1.59 to 2.75; 5 studies including 1547 patients; I², 5.9%). The pooled early mortality rate among patients aged 70 years or older was 78.8% (95% CI, 74.1 to 83.5; 6 studies including 617 patients; I², 41.8%). Two studies reported 1-year mortality (including hospital mortality) of 79.9% and 75.6%, respectively, in patients 70 years old or older. Conclusions: Advanced age should not be considered a contraindication for postcardiotomy VA-ECMO. However, in view of the high risk of early mortality, meaningful scrutiny is needed before using VA-ECMO after cardiac surgery in older patients.

Published
2019

47. Relationships among electrolyzed water postharvest treatments on winegrapes and chloroanisoles occurrence in wine

Author

Giacosa, S., Gabrielli, Mario, Torchio, Fabrizio, Rio Segade, S., Moar Grobas, A. M., Ricauda Aimonino, D., Gay, P., Gerbi, V., Maury, C., Rolle', Luca, Gabrielli M. (ORCID:0000-0002-9313-0140), Torchio F. (ORCID:0000-0002-7557-4346), Rolle L., Giacosa, S., Gabrielli, Mario, Torchio, Fabrizio, Rio Segade, S., Moar Grobas, A. M., Ricauda Aimonino, D., Gay, P., Gerbi, V., Maury, C., Rolle', Luca, Gabrielli M. (ORCID:0000-0002-9313-0140), Torchio F. (ORCID:0000-0002-7557-4346), and Rolle L.

Abstract

Electrolyzed water (EW) has attracted much recent attention as a high-performance, new technology for its potential use in the food industry. However, the risk of postharvest EW treatments of winegrapes destined for winemaking is the chloroanisoles formation in the final product. In the present study, we investigated the impact of postharvest grape EW and storage treatments on the occurrence of these compounds in wine, 2,4,6-trichloroanisole (TCA) being the main compound responsible for the cork taint off-flavor due to its extremely low perception threshold. The results revealed that the presence of TCA in the wines depended on the concentration of residual free chlorine in the must from the EW treatment. Particularly, TCA was not detected in wine when active chlorine concentrations higher than 0.005 mg/L were present in the must. Chloroanisole and chlorophenol levels in wine were strongly affected not only by EW but also by grape storage conditions (temperature, time, sunlight exposition). The results showed that the 24-hours grape storage at a controlled temperature of 20 °C in the dark, after EW treatment, resulted in the highest TCA concentrations in wines (7.3 ± 2.7 ng/L), while storage and withering in sunlight seemed to mitigate its presence in wine. This could suggest that microbiological formation of chloroanisoles may have been influenced by the storage temperature and germicidal effect of UV light. Biochemical mechanism of chloroanisoles production in grapes and wine is unknown, but the combination of residual free chlorine on the grape surface and the controlled storage conditions in the dark may have positively influenced the bio-formation of chloroanisoles and therefore their presence in wine.

Published
2019

48. Microbes and Alzheimer' disease: Lessons from H. Pylori and GUT microbiota

Author

Franceschi, Francesco, Ojetti, Veronica, Candelli, Marcello, Covino, Marcello, Cardone, S., Potenza, Angelo Eugenio, Simeoni, Benedetta, Gabrielli, Maurizio, Sabia, Luca, Gasbarrini, Giovanni Battista, Lopetuso, Loris Riccardo, Scaldaferri, Franco, Rossini, Paolo Maria, Gasbarrini, Antonio, Franceschi, F. (ORCID:0000-0001-6266-445X), Ojetti, V. (ORCID:0000-0002-8953-0707), Candelli, M. (ORCID:0000-0001-8443-7880), Covino, M. (ORCID:0000-0002-6709-2531), Simeoni, B., Gabrielli, M., Gasbarrini, G., Lopetuso, L., Scaldaferri, F. (ORCID:0000-0001-8334-7541), Rossini, P. M. (ORCID:0000-0003-2665-534X), Gasbarrini, A. (ORCID:0000-0002-7278-4823), Franceschi, Francesco, Ojetti, Veronica, Candelli, Marcello, Covino, Marcello, Cardone, S., Potenza, Angelo Eugenio, Simeoni, Benedetta, Gabrielli, Maurizio, Sabia, Luca, Gasbarrini, Giovanni Battista, Lopetuso, Loris Riccardo, Scaldaferri, Franco, Rossini, Paolo Maria, Gasbarrini, Antonio, Franceschi, F. (ORCID:0000-0001-6266-445X), Ojetti, V. (ORCID:0000-0002-8953-0707), Candelli, M. (ORCID:0000-0001-8443-7880), Covino, M. (ORCID:0000-0002-6709-2531), Simeoni, B., Gabrielli, M., Gasbarrini, G., Lopetuso, L., Scaldaferri, F. (ORCID:0000-0001-8334-7541), Rossini, P. M. (ORCID:0000-0003-2665-534X), and Gasbarrini, A. (ORCID:0000-0002-7278-4823)

Abstract

OBJECTIVE: the role of microbes and chronic inflammation in the pathogenesis of Alzheimer' disease (AD) has been postulated by many authors. On the other hand, several studies have reported the main role of H. pylori infection and/or GUT microbiota alteration in promoting chronic inflammation, thus possibly influencing both occurrence and evolution of AD. In this article, we analyze the most important and recent studies performed on this field both on humans and animals and provide possible pathogenic explanations. RESULTS: all main and most recent animal, human, epidemiological and in-silico studies, showed a role of H. pylori and/or dysbiosis in AD, mostly through the promotion of systemic chronic inflammation and/or by triggering molecular mimicry mechanisms. In particular, H. pylori infection seems to be related to a poorer cognitive performance. CONCLUSIONS: Indeed, bacteria have been shown to affect neurodegeneration by promoting inflammation, inducing molecular mimicry mechanisms and accumulation of Aß into the brain. These findings open the way for H. pylori eradicating trials and/or GUT microbiota remodulating strategies. Therefore, further studies are now needed in order to test whether antibiotics, pre and/or probiotics may exert a beneficial effect in the prevention of AD.

Published
2019

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