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Author(s): Gatto, Emilia M; Walker, Ruth H; Gonzalez, Claudio; Cesarini, Martin; Cossu, Giovanni; Stephen, Christopher D; Balint, Bettina; Rodriguez-Violante, Mayela; Jankovic, Joseph; Morgante, Francesca; Jinnah, Hyder A; Rare Movement Disorders Study Group of the International Parkinson Disease, Movement Disorders Society | Abstract: Background and purposeDespite enormous advances in identifying genetic variants responsible for many neurological diseases, access to genetic testing may be limited in clinical practice. The objective of this study was to assess worldwide access to genetic tests for movement disorders and factors impacting their utilization.MethodsThe Rare Movement Disorders Study Group of the International Parkinson and Movement Disorder Society designed an online survey electronically mailed to all 7815 members.ResultsSurvey data completed by 1269 participants from 109 countries were analysed. Limited access to geneticists and genetic counsellors was reported in many world regions compared to Europe and North America. Availability of genetic testing was limited, with rates of access lower than 50%. Genetic testing for chorea was the most commonly available. For parkinsonism, dystonia, ataxia, hereditary spastic paraplegias and metabolic disorders, there was limited access to genetic testing in all countries compared to Europe and North America, with significant differences found for Africa, Central/South America, Asia. In many regions, genetic testing was supported by either private or public funding. Genetic testing was free of charge in Europe according to 63.5% of respondents. In North America, Africa, Central/South America, Asia and the Middle East access to free of charge genetic testing was by far significantly lower compared to Europe.ConclusionsThis survey highlights difficulties in accessing genetic testing and individuals with expertise in genetics at the worldwide level. In addition, major disparities in genetic testing amongst world regions are highlighted, probably due to a variety of factors including financial barriers.

Most research in comparative cognition focuses on measuring if animals manage certain tasks; fewer studies explore how animals might solve them. We investigated bumblebees’ scanning strategies in a numerosity task, distinguishing patterns with two items from four and one from three, and subsequently transferring numerical information to novel numbers, shapes, and colors. Video analyses of flight paths indicate that bees do not determine the number of items by using a rapid assessment of number (as mammals do in “subitizing”); instead, they rely on sequential enumeration even when items are presented simultaneously and in small quantities. This process, equivalent to the motor tagging (“pointing”) found for large number tasks in some primates, results in longer scanning times for patterns containing larger numbers of items. Bees used a highly accurate working memory, remembering which items have already been scanned, resulting in fewer than 1% of re-inspections of items before making a decision. Our results indicate that the small brain of bees, with less parallel processing capacity than mammals, might constrain them to use sequential pattern evaluation even for low quantities.

Background Parkinson's disease is the second most common neurodegenerative disorder and affects people from all ethnic backgrounds, yet little is known about the genetics of Parkinson's disease in non-European populations. In addition, the overall identification of copy number variants at a genome-wide level has been understudied in Parkinson's patients. The objective of this study was to understand the genome-wide burden of copy number variants in Latinos and its association with Parkinson's disease. Methods We used genome-wide genotyping data from 747 Parkinson's disease patients and 632 controls from the Latin American Research Consortium on the Genetics of Parkinson's disease. Results Genome-wide copy number burden analysis showed that patients were significantly enriched for copy number variants overlapping known Parkinson's disease genes compared with controls (odds ratio, 3.97; 95%CI, 1.69-10.5; P = 0.018). PRKN showed the strongest copy number burden, with 20 copy number variant carriers. These patients presented an earlier age of disease onset compared with patients with other copy number variants (median age at onset, 31 vs 57 years, respectively; P = 7.46 × 10-7 ). Conclusions We found that although overall genome-wide copy number variant burden was not significantly different, Parkinson's disease patients were significantly enriched with copy number variants affecting known Parkinson's disease genes. We also identified that of 250 patients with early-onset disease, 5.6% carried a copy number variant on PRKN in our cohort. Our study is the first to analyze genome-wide copy number variant association in Latino Parkinson's disease patients and provides insights about this complex disease in this understudied population. © 2020 International Parkinson and Movement Disorder Society.

Las aceitunas de la variedad Arauco se colocaron con lejía al 2,25% durante ocho horas en bidones por triplicado. La lejía se reutilizó previa restitución del título hasta diez veces. Luego se lavaron con agua estanca durante cuatro y doce horas. Finalmente, las aceitunas se colocaron en salmuera acidificada al 0,2% 0 de ácido clorhídrico y ácido acético para la correspondiente fermentación láctica. El primer desamarizado sin reuso se tomó como testigo. A las muestras extraídas se le realizaron los siguientes análisis: DBO5, DQO, conductividad, fenoles, sólidos sedimentables. Se siguieron las diez fermentaciones con análisis físico-químico: pH, acidez, lejía residual y microbiológico. Del análisis de los datos se concluye que se puede reusar la lejía hasta cinco veces sin afectar las características organolépticas de las aceitunas. Fil: Maldonado, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gatto, E. Universidad Católica de Cuyo. Facultad Don Bosco; Argentina Fil: Callejón, D. Universidad Católica de Cuyo. Facultad Don Bosco; Argentina Fil: Barbero, M.A. Universidad Católica de Cuyo. Facultad Don Bosco; Argentina Fil: Crespo, Diana Elvira. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Santi, A. Instituto Nacional de Tecnología Industrial; Argentina Fil: Cerchiai, E. Instituto Nacional de Tecnología Industrial; Argentina

Interactions between peptides are relevant from a biomedical point of view, in particular for the role played by their aggregates in different important pathologies, and also because peptide aggregates represent promising scaffolds for innovative materials. In the present article, the aggregation properties of the homo-peptides formed by α-aminoisobutyric acid (U) residues are discussed. The peptides investigated have chain lengths between six and 15 residues and comprise benzyl and naphthyl groups at the N- and C-termini, respectively. Spectroscopic experiments and molecular dynamics simulations show that the shortest homo-peptide, constituted by six U, does not exhibit any tendency to aggregate under the conditions examined. On the other hand, the homologous peptide with 15 U forms very stable and compact aggregates in 70/30(v/v) methanol/water solution. Atomic force microscopy images indicate that these aggregates promote formation of long fibrils once they are deposited on a mica surface. The aggregation phenomenon is mainly due to hydrophobic interactions occurring between very stable helical structures, and the aromatic groups in the peptides seem to play a minor role.

Background: Ischemic stroke is the leading cause of mortality worldwide and a major contributor to neurological disability and dementia. Terutroban is a specific TP receptor antagonist with antithrombotic, antivasoconstrictive, and antiatherosclerotic properties, which may be of interest for the secondary prevention of ischemic stroke. This article describes the rationale and design of the Prevention of cerebrovascular and cardiovascular Events of ischemic origin with teRutroban in patients with a history oF ischemic strOke or tRansient ischeMic Attack (PERFORM) Study, which aims to demonstrate the superiority of the efficacy of terutroban versus aspirin in secondary prevention of cerebrovascular and cardiovascular events. Methods and Results: The PERFORM Study is a multicenter, randomized, double-blind, parallel-group study being carried out in 802 centers in 46 countries. The study population includes patients aged ≥55 years, having suffered an ischemic stroke (≤3 months) or a transient ischemic attack (≤8 days). Participants are randomly allocated to terutroban (30 mg/day) or aspirin (100 mg/day). The primary efficacy endpoint is a composite of ischemic stroke (fatal or nonfatal), myocardial infarction (fatal or nonfatal), or other vascular death (excluding hemorrhagic death of any origin). Safety is being evaluated by assessing hemorrhagic events. Follow-up is expected to last for 2–4 years. Assuming a relative risk reduction of 13%, the expected number of primary events is 2,340. To obtain statistical power of 90%, this requires inclusion of at least 18,000 patients in this event-driven trial. The first patient was randomized in February 2006. Conclusions: The PERFORM Study will explore the benefits and safety of terutroban in secondary cardiovascular prevention after a cerebral ischemic event.

The impact of urban air pollution on human health has become an important problem, and the estimation of the amount of pollutants to which people are exposed is a major chanllenge. Usually, population exposure is only related to outdoor pollutant concentration, however people spend the most time inside buildings. In this context, the objective of this contribution is to relate the outdoor pollutant concentration at pedestrian level with the concentration inside a standard building. Computational Fluid Dynamics (CFD) simulations of pollutant dispersion are performed in an array of buildings where the interior of the central building (target building) is also simulated. Traffic-related pollutant are considered and several configurations of open/closed windows under different wind directions are modelled. The relationship between indoor concentration in each room and the spatially-averaged pollutant concentration in the street at pedestrian level is investigated. The results show a large variability of indoor concentrations depending on the configuration of open/closed windows, the floor of the room and the room location and the incidence angle of wind. In general, indoor concentration decreases with floor and indoor concentration is lower than average concentration in the street. However, for some conditions in the first-floor rooms indoor concentration is higher than the spatially-averaged concentration in the street at pedestrian level. Taking the average over all cases the indoor-outdoor concentration ratio is 0.6±0.2.

Amyloid fibrils are formed by a model surfactant-like peptide (Ala)10-(His)6 containing a hexa-histidine tag. This peptide undergoes a remarkable two-step self-assembly process with two distinct critical aggregation concentrations (cac’s), probed by fluorescence techniques. A micromolar range cac is ascribed to the formation of prefibrillar structures, whereas a millimolar range cac is associated with the formation of well-defined but more compact fibrils. We examine the labeling of these model tagged amyloid fibrils using Ni-NTA functionalized gold nanoparticles (Nanogold). Successful labeling is demonstrated via electron microscopy imaging. The specificity of tagging does not disrupt the β-sheet structure of the peptide fibrils. Binding of fibrils and Nanogold is found to influence the circular dichroism associated with the gold nanoparticle plasmon absorption band. These results highlight a new approach to the fabrication of functionalized amyloid fibrils and the creation of peptide/nanoparticle hybrid materials., Biomacromolecules, 15 (9), ISSN:1525-7797, ISSN:1526-4602

The Apulian Region (Italy) is a socio-ecological system shaped by the millennial co-evolution between human actions and ecological processes. It is characterized by monumental olive groves protected from Regional Law 14/2007 for the cultural value of the landscape, currently threatened by the spread of a devastating phytopathogen, the bacteria Xylella fastidiosa. The aim of this paper is to apply landscape resilience analysis focusing on ecosystem services to understand the potential effects and trade-offs of regeneration policies in a peri-urban area characterized by monumental olive groves land cover. The study involved land-cover and land-use analysis, supported by a survey on the inhabitants and an ecosystem services analysis. The results showed a mismatch between the agroecosystem and the social and economic use linked to leisure or hospitality. The study area was defined as a peri-urban landscape characterized by tourist use. From the interviews of the users, the cultural heritage of olive groves seems linked to the presence of olive trees like a status quo of the landscape and olive oil productions. The culture aspect could thus be preserved by changing the type of olive trees. In addition, the analysis showed that the microclimate could be preserved and enhanced in terms of air temperature and thermal comfort, by replacing the olive trees with varieties resistant to Xylella, such as cv. Leccino. Therefore, regeneration policies that promote replacing dead olive groves with new olive trees could be efficient to stimulate social components of the landscape and improve the resilience of ecosystem services in peri-urban areas in the interest of the cultural heritage of the users and benefits that they provide. An ecosystem services analysis at a local scale could be a strategy for an integrated regenerate approach between land-use and land-cover with social, ecological, and economic evolutions vision orientated to a sustainable and desirable future.

This study carries out a quantitative analysis of the impact on microclimate (air temperature and thermal comfort) of a row of 165 historical Pinus pinea L. located in a central neighbourhood of Rome (Italy). The analysis starts from a qualitative general analysis on the stressful conditions leading to tree decline in the urban environment especially during extreme climate change phenomena. Subsequently, the effects of planting new types of trees are assessed using ENVI-met, a 3D prognostic non-hydrostatic model for the simulation of surface-plant-air interactions. Results, obtained by simulating three different scenarios in which the trees are first removed and then modified, show that a gradual renewal of the existing trees, based on priority criteria of maturity or senescence, vegetative and phytosanitary conditions, efficiency of ecosystem services and safety for citizens, has positive effects on thermal comfort. By integrating current results and scientific literature, the final aim of this work is to provide stakeholders with a strategic and systemic planning methodology, which, based on the innovative integrated use of tree management and modelling tools, may (i) enhance the benefits of greening in a scenario of climate change and (ii) lead to intervention strategies based on complementarity between conservation of existing trees and tree renewal.

This study analyses the interactions and impacts between multiple factors i.e., urban greening, building layout, and meteorological conditions that characterise the urban microclimate and thermal comfort in the urban environment. The focus was on two neighbourhoods of Lecce city (southern Italy) characterised through field campaigns and modelling simulations on a typical hot summer day. Field campaigns were performed to collect greening, building geometry, and microclimate data, which were employed in numerical simulations of several greening scenarios using the Computational Fluid Dynamics-based and microclimate model ENVI-met. Results show that, on a typical summer day, trees may lead to an average daily decrease of air temperature by up to 1.00 °C and an improvement of thermal comfort in terms of Mean Radiant Temperature (MRT) by up to 5.53 °C and Predicted Mean Vote (PMV) by up to 0.53. This decrease is more evident when the urban greening (in terms of green surfaces and trees) is increased by 1266 m2 in the first neighbourhood and 1988 m2 in the second one, with respect to the current scenario, proving that shading effect mainly contributes to improving the urban microclimate during daytime. On the contrary, the trapping effect of heat, stored by the surfaces during the day and released during the evening, induces an increase of the spatially averaged MRT by up to 2 °C during the evenings and a slight deterioration of thermal comfort, but only locally where the concentration of high LAD trees is higher. This study contributes to a better understanding of the ecosystem services provided by greening with regard to microclimate and thermal comfort within an urban environment for several hours of the day. It adds knowledge about the role of green areas in a Mediterranean city, an important hot spot of climate change, and thus it can be a guide for important urban regeneration plans.

This study investigates the effect of urban vegetation on thermal comfort in a neighbourhood of Lahti (Finland) by means of modeling simulations performed with the Computational Fluid Dynamics-based and microclimate model ENVI-met. A scenario without vegetation and the current one (with vegetation) are considered to assess the effects of vegetation in different seasons and provide suggestions for thermal comfort improvement.

This paper is devoted to the assessment of urban greening effects on two important ecosystem services, i.e., air quality and CO2 storage, including the corresponding economic impacts in a real urban area, i.e., a district located in the Mediterranean city of Lecce (southern Italy). Two tools were employed, i-Tree Canopy and the computational fluid dynamics (CFD) microclimate model ENVI-met. i-Tree Canopy allowed fully determining the land-cover percentage on the basis of different ground cover classes and obtaining an estimate of annual values of CO2 storage, air pollutant removal, and economic benefits in the presence of urban greening. The estimate in i-Tree Canopy considered only the amount of greening, therefore, air pollutant removal estimates were only potential. As the vegetation was located in street canyons, its interaction with local meteorology and urban geometry strictly affected the dispersion of nitrogen oxides (NOx) (taken here as an example) as obtained from ENVI-met simulations. In ENVI-met, both deposition/absorption and aerodynamic effects were considered, and local increases in concentration were found in the district. The analysis of results obtained from different tools (one complex (CFD model) and the other simple (i-Tree model)) showed the error associated with the simple model in the computation of impacts if the interaction among the vegetation characteristics, the meteorological conditions, and the urban geometry was neglected, however, it also uncovers a novel approach for comprehensively characterizing a given area in terms of its vegetation cover, CO2 storage, and economic benefits, as well as local effects on air quality. This study is set in a broader context aimed at assessing the air quality in urban canopies of Mediterranean areas characterized by the presence of narrow street canyons where pollutants can accumulate due to ineffective air exchange with the above atmosphere.

This paper presents a conceptual framework that looks at photovoltaic systems in synergy with ecosystem services. The focus is to connect business success with social and ecological progress based on the operative concept of multifunctional land use. Such an approach attempts to harmonise the needs of the industrial processes of photovoltaic systems and the ecological and social needs of the landscape context. Different from the usual design of ground photovoltaic systems in farmlands or brownfields, a new framework is proposed, combining photovoltaic panels and vegetation. A case study is considered, applying the framework to existing photovoltaic systems in the Apulia region (southern Italy). The analysis shows how the framework has, among others, the major functions of increasing solar energy production, recycling wastewater, creating raw material for biofuel, as well as providing animal habitat and mitigating air temperature. The latter is preliminarily evaluated by means of modelling simulations performed with a computational fluid dynamics and microclimate model, ENVI-met. This approach opens up a new vision of the infrastructure design of photovoltaic systems which can produce new social and economic income.

This paper is devoted to the quantification of changes in ventilation of a real neighborhood located in Pamplona, Spain, due to the presence of street trees Pollutant dispersion in this urban zone was previously studied by means of computational fluid dynamic (CFD) simulations. In the present work, that research is extended to analyze the ventilation in the whole neighborhood and in a tree-free street. Several scenarios are investigated including new trees in the tree-free street, and different leaf area density (LAD) in the whole neighborhood. Changes between the scenarios are evaluated through changes in average concentration, wind speed, flow rates and total pollutant fluxes. Additionally, wind flow patterns and the vertical profiles of flow properties (e.g., wind velocity, turbulent kinetic energy) and concentration, horizontally-averaged over one particular street, are analyzed. The approach-flow direction is almost perpendicular to the street under study (prevailing wind direction is only deviated 4&ordm, from the perpendicular direction). For these conditions, as LAD increases, average concentration in the whole neighborhood increases due to the decrease of wind speed. On the other hand, the inclusion of trees in the street produces an increase of averaged pollutant concentration only within this street, in particular for the scenario with the highest LAD value. In fact, the new trees in the street analyzed with the highest LAD value notably change the ventilation producing an increase of total pollutant fluxes inward the street. Additionally, pollutant dispersion within the street is also influenced by the reduction of the wind velocity along the street axis and the decrease of turbulent kinetic energy within the vegetation canopy caused by the new trees. Therefore, the inclusion of new trees in a tree-free street should be done by considering ventilation changes and traffic emissions should be consequently controlled in order to keep pollutant concentration within healthy levels.

This paper is devoted to the analysis of the impact of changes in olive urban forests affected by Xylella fastidiosa on ecosystem services. The focus is on microclimate and thermal comfort evaluated by two indices: the temperature of equivalent perception (TEP) and the predicted mean vote (PMV), which take into account both microclimate parameters and personal factors (heat resistance of clothing and human activity). The work has been carried out through (i) a qualitative analysis of the potential ecosystem services changes caused by temporary transition from olive groves to uncultivated soil, (ii) a study of the potential change of land use from monumental olive groves to other types of use, and (iii) a quantitative analysis on microclimate impact due to the loss of ecosystem services in two selected neighborhoods located in the Apulia region and chosen due to their proximity to the urban context. The analysis revealed that (i) direct effects on ecosystem services are principally linked with regulation functions and cultural services, (ii) a critical loss of cultural value of monumental olive groves occurred in the two neighborhoods, (iii) such a loss may lead to an increase of TEP and PMV, indicating a decrease of thermal comfort in the whole neighborhoods. Thus, it is necessary to plan the replanting policies of the use of the areas affected by X. fastidiosa not only in terms of agricultural planning but also in terms of landscape, urban planning, and human well-being.

This paper is devoted to the application of the modelling approach, as one of the methods for the evaluation of thermal comfort, to neighborhoods located in two cities characterized by a different climate, i.e., a Mediterranean city in southern Italy (Lecce) and a northern European city in southern Finland (Lahti). The impact of the presence of vegetation in both places is evaluated and compared, further considering alternative scenarios for thermal comfort improvement. The thermal comfort condition is expressed in terms of indices (mean radiant temperature (MRT) and predicted mean vote (PMV)). Results show that at pedestrian level the presence of vegetation lead to an improvement of thermal comfort in summer of about 2 points in both neighborhoods. This improvement is also evident observing the spatial distribution of MRT with a difference of 7 &deg, C in the Lecce neighborhood and 3 &deg, C in Lahti. In winter, thermal discomfort is observed in the presence of vegetation with a difference of 1.3 &deg, C in the Lecce neighborhood and 1.5 &deg, C in Lahti in terms of MRT. However, trees and green cover have the important potential to offset climate change impact and to make urban environments less thermally stressful. This study aims to guide urban planners towards a motivated and necessary transaction towards new green infrastructure whose effect should, however, be analyzed and investigated case by case.

BACKGROUND: Condylar hyperplasia of the mandible is an uncommon idiopathic disorder of the jaw characterised by increased volume of the condyle, unilaterally or bilaterally, leading to facial asymmetry, mandibular deviation, malocclusion and articular dysfunction. CASE REPORT: The authors present one case of unilateral condylar hyperplasia of a 16-year-old patient affected by severe facial asymmetry. Conventional X-rays examinations, multislice spiral CT and bone SPECT were used for the final diagnosis of primary condyle hyperplasia. The patient was treated with a combined orthodontic and surgical approach. CONCLUSION: Treatment of condylar hyperplasia with a combined orthodontic and surgical approach including condylectomy yield good aesthetic and functional outcomes.

Background The aim of this study was to evaluate the amount of radiation doses absorbed by soft tissues (entrance skin dose) with a low-dose spiral computed tomography (CT) protocol compared to conventional X-ray techniques commonly used in orthodontics. Methods The amount of skin dose has been evaluated using a tissue-equivalent head-neck radiotherapy humanoid phantom with thermoluminescent dosimeters placed at the level of eye lens, parotid glands, and thyroid glands. CT images have been taken using a Sensation 16 Siemens CT scan and a low-dose protocol (15 mAs, 1 pitch, 2.5 mGy (CTDIvol), 80 kV, 1-mm slice thickness). Results The difference in image quality between traditional X-ray techniques and low-dose spiral CT was statistically significant (P Conclusions Our protocol allows a more accurate orthodontic diagnosis without an increase of radiological risk for the patients in comparison to traditional X-ray techniques.