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1. Method for the production of hydroxylated collagen-like compounds using prolyl hydroxylase from Hansenula polymorpha

Author

de Bruin, E.C., Werten, M.W.T., and de Wolf, F.A.

Subjects
Komagataella pastoris, Microorganism, Collagens, preparation, Kluyveromyces lactis, Pichia angusta, Fungi, Proteins, Saccharomyces cerevisiae, Instituut voor Agrotechnologisch Onderzoek, Gelatins, processes, Hydroxylation, Glyoproteins, Agrotechnological Research Institute, Cryptococcus curvatus
Abstract

The present invention relates to a method for the production of collagen-like compounds containing hydroxylated proline residues. Of specific interest is the production of recombinant collagen-like compounds in which hydroxylation of proline residues is achieved by a prolyl hydroxylase from a fungus, preferably a yeast, in particular Hansenula polymorpha. Also the invention concerns a method for controlling the hydroxylation of proline residues by such a prolyl hydroxylase characterised by the addition of collagen-like oligopeptides, such as gelatine hydrolysate, in particular gelatone or peptone.

Published
2002

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