Search

Glyptapanteles niveus species group The G. niveus species group can be separated from the other described Glyptapanteles from Australia by having the gena without a pale spot, T1 and T2 dark, propodeum coarsely rugose and the anteromesoscutum with very sparse deep punctures, always with some smooth areas larger than the diameter of punctures, face strongly punctured and T1 not wedge-shaped. All the described species also have a vividly white fore coxa, mid coxa, hind trochanter and anterior metasomal sternites. The species group currently contains three described species: G. bradfordae Fagan-Jeffries, Bird & Austin sp. nov., G. cooperi Fagan-Jeffries, Bird & Austin sp. nov. and G. niveus Fagan-Jeffries, Bird & Austin sp. nov. All three species also have larger eyes (and consequently, smaller malar spaces) than most other species of Glyptapanteles from Australia. This species group is currently only known from eastern QLD, from Kuranda (northern QLD) and from near Brisbane (southern QLD) (Fig. 4F). Unplaced species of Glyptapanteles from Australasia There are several species of Glyptapanteles from Australasia which we do not place within a species group, either because they do not form a monophyletic group with other species with which they share a clear morphological character, or because they are previously described species without molecular data and/or without clear morphological affinities to any of the assigned species groups. This includes G. afiamaluanus (Fullaway, 1941), G. artonae (Rohwer, 1926), G. aucklandensis (Cameron, 1909), G. baylessi Fagan-Jeffries, Bird &Austin sp. nov., G. deliasa Austin & Dangerfield, 1992, G. drioplanetus Fagan-Jeffries & Austin, 2021, G. fullawayi Austin & Dangerfield, 1992, G. kurandaensis Fagan- Jeffries, Bird & Austin sp. nov., G. operculinae (Fullaway, 1941), G. phytometrae (Wilkinson, 1928) and G. taylori (Wilkinson, 1928). Of these unplaced species, only G. baylessi sp. nov., G. deliasa, G. drioplanetus and G. kurandaensis sp. nov. are found in Australia. Species treatments

Glyptapanteles ruhri Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 983624B0-EA3C-4AA3-B31B-54955164B7CB Figs 13B, 50 Diagnosis Glyptapanteles ruhri sp. nov. is in the G. arcanus species group and can be separated from the other members of the species group, other than from Glyptapanteles rodriguezae sp. nov., by having the hind femur pale in colouration, the propodeum punctate with smooth areas between punctures and with little-tono rugosity and T1 punctured in the posterior half. There was no external morphological character found which easily separates G. ruhri sp. nov. from G. rodriguezae sp. nov. and we do not provide a morphological diagnosis between these two species. The two species are sister lineages in the current phylogeny (Fig. 2), but Glyptapanteles ruhri sp. nov. is 6 bp different in the wingless sequence from G. rodriguezae sp. nov. and>6% divergent in the COI barcode and we therefore feel confident that they are different species. Etymology Named for researcher Peter R��hr, who was part of the collection of the type specimen and contributed to the success of a field trip in northern QLD in 2019 that led to the collection of many important microgastrine specimens. Material examined Holotype AUSTRALIA ��� ♀; Queensland, Hallorans Hill Lookout, Atherton; -17.268, 145.4917268; 858 m a.s.l.; 21 Nov. 2019; E. Fagan-Jeffries, J.B. Dorey and P. R��hr leg.; sweeping vegetation; Extraction1198, BOLD: AUGLY071-21; QM T250986. Paratypes AUSTRALIA ��� Queensland ��� 1 ♂; Woonoonooroon National Park scenic drive; -17.585, 145.7028957; 674 m a.s.l.; 21 Nov. 2019; E. Fagan-Jeffries, J.B. Dorey and P. R��hr leg.; sweeping vegetation; Extraction832, BOLD: AUGLY001-21; QM T250987 ��� 1 ♂; same collection data as for preceding; Extraction844, BOLD: AUGLY002-21; QM T250988 ��� 1 ♀ (ethanol); Lamington National Park; -28.21, 153.139; 474 m a.s.l.; 9���19 Apr. 2007; C. Lambkin and N. Starick leg.; IBISCA Plot # IQ- 500-C rainforest Malaise trap; Extraction636, BOLD: AUMIC418-18; QM T208406 ��� 1 ♀; Lamington National Park; -28.148, 153.137; 267 m a.s.l.; 8���18 Jul. 2007; C. Lambkin and N. Starick leg.; IBISCA Plot # IQ-300-A rainforest Malaise trap; Extraction639, BOLD: AUMIC420-18; QM T208407. Description Female COLOURATION. Gena without a pale spot; labrum pale or reddish-brown; scape colour in ventral half uniformly paler than flagellomeres;flagellomeres all black/dark brown or uniformly reddish-brown; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark or dark with significant orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; fore coxa white; mid coxa white; hind coxa dark; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark; T2 sclerotised area dark or dark reddish-brown; T2 lateral area same colour as sclerotised area, or only slightly paler or dark extends past indentation, but then pale; T3 dark or uniformly brown; T4+ dark or reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 1.9 mm; fore wing length 2.1 mm; antennal length slightly longer than body length. HEAD. Antennal flagellomere 14 length/width 1.57���2.00; antennal flagellomere 2 length/width 2.62��� 3.00; OOD/POD 1.57���1.71; IOD/POD 1.43. MESOSOMA. Anteromesoscutum sculpturing with very sparse, deep punctures; scutellar disk sculpturing with only very shallow punctures, smooth and shiny; 9���10 pits in scutellar sulcus; propodeum with median carina absent, irregular rugose sculpturing along centre line with punctures in anterior half of propodeum, postero-lateral areas smooth or median carina absent, propodeum mostly punctate with some irregular shallow rugosity in centre, postero-lateral corners smooth and shiny. WINGS. Pterostigma length 0.53 mm; pterostigma width 0.16 mm; r 0.22 mm; 2RS 0.14 mm; 2m 0.08 mm; (RS+M)b 0.06 mm. METASOMA. T1 lateral edges parallel for anterior 3/4 of length, then narrowing posteriorly; T1 mostly smooth, some punctures in posterior half; T1 length 0.30 mm; T1 width at posterior edge 0.14 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny, or smooth with some very shallow punctures in posterior half; T2 length 0.14 mm; T2 width at posterior edge 0.2 mm; ovipositor slightly protruding from end of metasoma. Male As female, 7���10 pits in scutellar sulcus. Remarks Glyptapanteles ruhri sp. nov. constitutes BIN BOLD:ADL3583 and is 6.25% (p-dist.) divergent from the closet BIN in the database (BOLD:AAU5026; Glyptapanteles rodriguezae sp. nov.). Using the BOLD Batch ID engine, the COI barcode of the holotype is 6.4% different from the most similar COI sequence from an Australian specimen (BOLD: ASQAS187-11, which was not included in the original study and AUGLY062-21; Glyptapanteles rodriguezae sp. nov.). All five type specimens were sequenced for the wingless gene, which is identical among the type specimens and differs by a minimum of 6 bp from all other species with available sequence data. Distribution This species is known from a significant stretch of eastern QLD, from Atherton in northern QLD to Lamington National Park, south of Brisbane., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 101-103, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles ferrugineus Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: FF8A3C0E-096B-40AE-ADA3-33E4069EF15E Figs 9A, 34 Diagnosis Glyptapanteles ferrugineus sp. nov. is in the G. albigena species group and can be separated from the other members of the species group by the pale spot on the gena being small but clearly visible, T2 dark, propodeal median carina completely absent, hind femur light brown to pale and mesosoma with a strong dark red tinge. Etymology The species epithet, ��� ferrugineus ���, is a Latin adjective for ���rust-coloured��� and refers to the red tinge for the mesosoma. Material examined Holotype AUSTRALIA ��� ♀; Western Australia, 23 km NNW of Albany; -34.8532, 117.809; 16 Nov. 2000; T.Simmul and S. Cunningham leg.; Malaise in remnant vegetation; Extraction144, BOLD: AUMIC042-18; ANIC 32 130189. Description Female COLOURATION. Gena with a pale spot; labrum reddish-brown; scape colour in ventral half uniformly paler than flagellomeres; flagellomeres uniformly reddish-brown; tegula pale; wing veins uniformly pale; anteromesoscutum dark with red tinge; scutellar disk and metanotum dark with red tinge; propodeum dark with red tinge; fore coxa pale yellow; mid coxa pale yellow; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow; mid tibia pale yellow; hind basitarsus light brown; T1 dark reddish-brown; T2 sclerotised area dark reddish-brown; T2 lateral area dark, extends past indentation, but then pale; T3 mostly dark with paler lateral areas; T4+ reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 2.2 mm; fore wing length 2.1 mm; antennal length slightly shorter than body length. HEAD. Antennal flagellomere 14 length/width 1.66; antennal flagellomere 2 length/width 3.33; OOD/ POD 1.71; IOD/POD 1.57. MESOSOMA. Anteromesoscutum sculpturing with shallow punctures, space between punctures generally smaller than diameter of punctures; scutellar disk sculpturing with only very shallow punctures; eight pits in scutellar sulcus; propodeum with median carina absent, very smooth and shiny, only very shallow punctures associated with setae. WINGS. Pterostigma length 0.48 mm; pterostigma width 0.22 mm; r 0.12 mm; 2RS 0.1 mm; 2m 0.13 mm; (RS+M)b 0.07 mm. METASOMA. T1 wedge-shaped, narrowing posteriorly for entirety of length, lateral edges straight (but not parallel); T1 smooth and shiny; T1 length 0.31 mm; T1 width at posterior edge 0.07 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.13 mm; T2 width at posterior edge 0.21 mm; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks Glyptapanteles ferrugineus sp. nov. constitutes BIN: BOLD:ADL4089 and is 4.17% (p-dist.) divergent from the closet BIN in the database (BOLD:ABA6213; Glyptapanteles austrinus sp. nov.). Using the BOLD Batch ID engine, the COI barcode of the holotype is 4.0% different from the most similar COI sequence from an Australian specimen (HYAT371-11; Glyptapanteles austrinus sp. nov.). The holotype was able to be sequenced for the wingless gene, which differs by a minimum of 5 bp from all other species with available sequence data. Distribution This species is known only from Albany in southern WA., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 62-64, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles albigena Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: B83B4BAE-3154-4717-A00E-EB6FC9EFF9CD Figs 5A, 17 Diagnosis Glyptapanteles albigena sp. nov. is in the G. albigena species group and can be separated from other members of the group by the gena having a very large pale spot, covering at least a third of the gena height. Etymology The species epithet ‘ albigena ’ is from the Latin adjective ‘album’ (meaning ‘white’) and refers to the pale spot on the gena that is characteristic of this species and the broader species group. Material examined Holotype AUSTRALIA • ♀; New South Wales, Werrikimbe National Park, Plateau Beach Cpg; 31.1807, 152.3266; 1055 m a.s.l.; 17–20 Jan. 2018; K.M. Bayless leg.; sweep ferns along trail; Extraction1110, BOLD: AUGLY048-21; ANIC 32 130334. Paratypes AUSTRALIA – Australian Capital Territory • 1 ♂; Brindabella, Blundells Creek; -35.416667, 148.833333; 8 Jan. 2018; K.M. Bayless leg.; sweep; Extraction1185, BOLD: AUGLY070-21; AM K.379877. – New South Wales • 1 ♂; same collection data as for holotype; Extraction1177, BOLD: AUGLY068-21; ANIC 32 130335 • 1 ♂; same collection data as for holotype; Extraction1178, BOLD: AUGLY069-21; ANIC 32 130336 • 1 ♀ (ethanol); Monga National Park, near Penance Grove Walk; -35.597372, 149.912126; 9 Nov. 2019 – 16 Jan. 2020; K.M. Bayless and J.G. Lumbers leg.; Malaise trap over stream, trap destroyed by bushfire; 30 Dec. 2019; partially dried out; Extraction1664, BOLD: AUGLY126-21; ANIC 32 130337 • 1 ♂ (ethanol); Budawang National Park, Mt Budawang Road; -35.4563, 149.9955; 750 m a.s.l.; 16 Jan.–10 Feb. 2019; K.M. Bayless and X. Li leg.; Malaise over Feagans Creek; Extraction1634, BOLD: AUGLY120-21; ANIC 32 130338 • 1 ♀; Barren Grounds NR, 21 m NE of Barren Ground Rd; -34.66971, 150.71167; 1–6 Feb. 2020; K.M. Bayless and J.G. Lumbers leg.; Malaise trap, wet sclerophyll forest; Extraction872, BOLD: AUGLY013-21; ANIC 32 130339 • 1 ♀; same collection data as for preceding; Extraction1136, BOLD: AUGLY061-21; ANIC 32 130340 • 1 ♂; same collection data as for preceding; Extraction871, BOLD: AUGLY012-21; ANIC 32 130341 • 1 ♂; same collection data as for preceding; Extraction1133, BOLD: AUGLY060-21; ANIC 32 130342 • 1 ♂ (ethanol); same collection data as for preceding; 23–29 Jan. 2020; Extraction1607, BOLD: AUGLY114-21; ANIC 32 130343 • 1 ♂; same collection data as preceding; Extraction1609, BOLD: AUGLY115-21; ANIC 32 130344 • 1 ♂ (ethanol); same collection data as for preceding; Extraction1617, BOLD: AUGLY117-21; ANIC 32 130345. – Queensland • 1 ♀; Lamington National Park; -28.259, 153.162; 1142 m a.s.l.; 11–21 Mar. 2007; C Lambkin and N. Starick leg.; IBISCA Plot # IQ-1100-B rainforest Malaise trap; Extraction609, BOLD: AUMIC394-18; QM T208405. Description Female COLOURATION. Gena with a pale spot; labrum pale; scape colour in ventral half paler than flagellomeres at proximal end; flagellomeres all black/dark brown; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark, dark with significant orange patches on posterolateral corners or dark with very slight orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; fore coxa pale yellow; mid coxa pale yellow; hind coxa dark; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark or dark reddishbrown; T2 sclerotised area dark or dark reddish-brown; T2 lateral area dark, extends past indentation, but then pale; T3 dark, mostly pale with darker patch in centre or mostly dark with paler lateral areas; T4+ dark or reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 2.3 mm; fore wing length 2.4 mm; antennal length similar to body length. HEAD. Antennal flagellomere 14 length/width 1.71–2.16; antennal flagellomere 2 length/width 3.00– 3.71; OOD/POD 1.67–1.71; IOD/POD 1.33–1.57. MESOSOMA. Anteromesoscutum sculpturing with shallow to deep punctures, space between punctures a mixture of smaller than diameter of punctures and of similar size. Often smoother in posterior centre or shallow punctures, space between punctures generally smaller than diameter of punctures; scutellar disk sculpturing with shallow or very shallow punctures scattered over most of area; 6–8 pits in the scutellar sulcus; propodeum with median carina faintly indicated for all of length or sometimes only in posterior half; rest of propodeum either smooth with some punctures in anterior half, or smooth with some shallow rugosity, or smooth with both punctures and some shallow rugosity. WINGS. Pterostigma length 0.57 mm; pterostigma width 0.23 mm; r 0.16 mm; 2RS 0.15 mm; 2m 0.14 mm; (RS+M)b 0.09 mm. METASOMA. T1 wedge-shaped, narrowing posteriorly for entirety of length, lateral edges straight (but not parallel); T1 smooth and shiny, some shallow scattered punctures on lateral edges or smooth and shiny, some shallow scattered punctures on lateral edges and posterior half; T1 length 0.36 mm; T1 width at posterior edge 0.07 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.15 mm; T2 width at posterior edge 0.26 mm; ovipositor slightly protruding from end of metasoma. Male As female, but antenna slightly longer than body. Remarks Glyptapanteles albigena sp. nov. constitutes BIN: BOLD:ADL3908 and is 5.48% (p-dist.) divergent from the closet BIN in the database (BOLD:ABA6208; Glyptapanteles sanniopolus sp. nov.). Using the BOLD Batch ID engine, the COI barcode of the holotype is 6.3% different from the most similar COI sequence of an Australian specimen (HYAT425-11 and HYAT424-11; Glyptapanteles sanniopolus sp. nov.). All specimens able to be sequenced for the wingless gene share a unique barcode that differs by a minimum of 1 bp from all other species with available sequence data. Distribution This species is known from the ACT and NSW and from southern QLD.

Glyptapanteles wrightae Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 569C2146-14FF-4453-BA4E-A6A8787A044A Fig. 53 Diagnosis Glyptapanteles wrightae sp. nov. can be separated from the other members of the arcanus species group as follows: Glyptapanteles wrightae sp. nov. can be separated from G. rodriguezae sp. nov. and G. ruhri sp. nov. by T1 being smooth and shiny, not having punctures that cover at least a third of the area of the posterior half of the tergite. Glyptapanteles wrightae sp. nov. can be separated from G. goodwinnoakes sp. nov., G. erucadesolator sp. nov., G. lambkinae sp. nov., G. arcanus sp. nov. and G. vergrandiacus sp. nov. by the propodeum being less coarsely and less consistently rugose sculptured across the anterior half of the propodeum. Glyptapanteles wrightae sp. nov. has the propodeum with strong punctures in the anterior half, the posterior half with shallow or strong rugose sculpturing. Glyptapanteles wrightae sp. nov. can be separated from G. doreyi sp. nov. by the tegula being pale in colouration (the tegula in G. doreyi sp. nov. is generally dark in colouration), the hind femur mostly pale or light brown (mostly dark in G. doreyi sp. nov.) and the indentation in the centre of the mesopleuron being smooth, not strongly canaliculated as it is in G. doreyi sp. nov. There was not a morphological character found that easily separates G. wrightae sp. nov. from G. lessardi sp. nov. and we do not diagnose these species morphologically. The wingless barcodes of the two species differ by 3 bp and the COI is greater than 4% divergent. Etymology Named for Susan Wright, who collected the holotype specimen. EPF-J would like to acknowledge Susan’s support of visiting researchers at the QM and her continual generosity with her time and advice. Material examined Holotype AUSTRALIA • ♀; Queensland, Samsonvale Cemetery, 8.5 km SSE of Dayboro; -27.2703, 152.856; 50 m a.s.l.; 6 Jan.–8 Feb. 2015; S. Wright leg.; Casuarina /open forest Malaise trap; Extraction198, BOLD: AUMIC084-18; QM T208400. Paratypes AUSTRALIA – New South Wales • 1 ♂; Royal National Park, near Waterfall Couranga Track near Hacking River; -34.1486, 151.0221; 40 m a.s.l.; 20 Jan.–6 Feb. 2020; K.M. Bayless and J.G. Lumbers leg.; 6 m Malaise trap over Waterfall Creek; Extraction857, BOLD: AUGLY128-21; ANIC 32 130373 • 1 ♂; same collection data as for preceding; Extraction863, BOLD: AUGLY010-21; ANIC 32 130374. Description Female COLOURATION. Gena without a pale spot; labrum reddish-brown; scape colour in ventral half uniformly paler than flagellomeres; flagellomeres darkening distally; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark; scutellar disk and metanotum dark; propodeum dark; fore coxa white; mid coxa white; hind coxa dark; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark; T2 sclerotised area dark reddish-brown; T2 lateral area dark extends past indentation, but then pale; T3 mostly dark with paler lateral areas; T4+ reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 1.8 mm; fore wing length 1.8 mm; antennal length slightly longer than body length. HEAD. Antennal flagellomere 14 length/width 3.5; antennal flagellomere 2 length/width 2.71; OOD/ POD 1.83; IOD/POD 1.67. MESOSOMA. Anteromesoscutum sculpturing with deep punctures, space between punctures a mixture of smaller than diameter of punctures and of similar size; scutellar disk sculpturing with deep, sparse, irregularly spaced punctures, more common on anterior lateral edges or scutellar disk with shallow punctures scattered over most of area; eight pits in scutellar sulcus; propodeum with median carina absent, strong punctures in anterior half, rugose sculpturing in posterior half. WINGS. Pterostigma length 0.49 mm; pterostigma width 0.16 mm; r 0.13 mm; 2RS 0.14 mm; 2m 0.06 mm; (RS+M)b 0.07 mm. METASOMA. T1 lateral edges parallel for anterior ½ to ⅔ of length, then narrowing posteriorly; T1 mostly smooth, some punctures in posterior half, smooth and shiny, sometimes with some shallow scattered punctures on lateral edges; T1 length 0.25 mm; T1 width at posterior edge 0.08 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.14 mm; T2 width at posterior edge 0.19 mm; ovipositor slightly protruding from end of metasoma. Male As female. Remarks Glyptapanteles wrightae sp. nov. constitutes BIN BOLD:ADL3293 and is 1.76% (p-dist.) divergent from the closet BIN in the database (BOLD:AEI5416; see information below about the specimens within this BIN). Using the BOLD Batch ID engine, the COI sequence of the holotype is 1.7% different from the most similar COI sequence from an Australian specimen (AUGLY139-21, an undescribed lineage, with three sequences). This closely related lineage, (BOLD:AEI5416; also including BOLD AUGLY135-21 and AUGLY130-21) requires further study to determine whether it is within the species limits of G. wrightae sp. nov. or whether it represents a distinct species. Distribution This species is currently known from southern QLD and north-eastern NSW.

Glyptapanteles austini species group The G. austini species group contains three species: G. austini Fagan-Jeffries & Bird sp. nov., G. guzikae Fagan-Jeffries, Bird & Austin sp. nov. and G. kingae sp. nov., which are a monophyletic, molecularly distinct lineage (Fig. 2). Two species in this group (G. guzikae sp. nov. and G. austini sp. nov.) are known from single localities in southern SA, whilst G. kingae sp. nov. has a broad distribution and is found in the northern half of WA, central SA and the ACT (Fig. 4C). The group can be separated from the other species of Glyptapanteles described from Australia by the following combination of characters: gena without a pale spot, T1–T2 dark, anteromesoscutum all dark and with punctures without smooth areas greater than the diameter of punctures (i.e., anteromesoscutum not as in the G. niveus species group (i.e., not as in Fig. 12B)), propodeum not coarsely rugose, T1 only parallel for at most ⅔ of length before narrowing posteriorly, mesoscutellar disk without dense, strong punctures, scape darker or the same colour as flagellomeres, tegula pale, labrum dark.

Glyptapanteles eburneus Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 814EBC38-DD57-4503-87B3-01C47A2A517D Figs 6B, 10A, 32 Diagnosis Glyptapanteles eburneus sp. nov. is in the G. eburneus species group and can be separated from G. foraminous sp. nov. by having T2 pale, the same colour as T1 (G. foraminous sp. nov. has T2 dark). Additionally, the wingless sequences of the two holotype specimens are distinct and differ by 5 bp. Etymology The species epithet ��� eburneus ��� is a Latin adjective for ���ivory��� and refers to the pale colouration of T1���2. Material examined Holotype AUSTRALIA ��� ♀; New South Wales, Royal National Park. south end, Lady Carrington Drive Rainforest; -34.1482, 151.031; 24 Nov. 1996; A.D. Austin leg.; Extraction 439, BOLD: AUMIC274-18; AM K.517935. Description Female COLOURATION. Gena without a pale spot; labrum pale; scape colour in ventral half uniformly paler than flagellomeres; flagellomeres darkening distally; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum dark with significant orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; fore coxa pale yellow; mid coxa pale yellow; hind coxa pale yellow; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 pale; T2 sclerotised area pale; T2 lateral area same colour as sclerotised area, or only slightly paler; T3 pale; T4 pale with dark patch in centre, then darkening posteriorly on T5���7. HOLOTYPE BODY MEASUREMENTS. Body length 2.7 mm; fore wing length 2.7 mm; antennal length slightly longer than body length. HEAD. Face densely sculptured, punctate reticulate; antennal flagellomere 14 length/width 2.16; antennal flagellomere 2 length/width 3.62; OOD/POD 1.75; IOD/POD 1.75. MESOSOMA. Anteromesoscutum sculpturing with deep punctures, space between punctures a mixture of smaller than diameter of punctures and of similar size; scutellar disk sculpturing with deep irregularly spaced punctures; nine pits in scutellar sulcus; propodeum with median carina present and complete, rest of propodeum mostly smooth. WINGS. Pterostigma length 0.62 mm; pterostigma width 0.23 mm; r 0.18 mm; 2RS 0.16 mm; 2m 0.10 mm; (RS+M)b 0.10 mm. METASOMA. T1 wedge-shaped, narrowing posteriorly for entirety of length, lateral edges straight (but not parallel); T1 mostly smooth, some punctures in posterior half; T1 length 0.4 mm; T1 width at posterior edge 0.12 mm; T2 with curved lateral and anterior edges, becoming arch- or semicircle-shaped; T2 smooth and shiny; T2 length 0.17 mm; T2 width at posterior edge 0.3 mm; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks Glyptapanteles eburneus sp. nov. constitutes BIN: BOLD:ADL5650 and is 2.4% (p-dist.) divergent from the closet BIN in the database (BOLD:ADL3303; Glyptapanteles foraminous sp. nov.). Using the BOLD Batch ID engine, the COI barcode of the holotype is 2.3% different from the most similar COI sequence from an Australian specimen (AUMIC069-18; Glyptapanteles foraminous sp. nov.). The type specimen was able to be sequenced for the wingless gene, which differs by a minimum of 5 bp from all other species with available sequence data. Distribution This species is currently represented by a single specimen from Royal National Park, just south of Sydney., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 57-59, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles albigena species group The G. albigena species group is easily separated from the other species of Glyptapanteles in Australasia by the gena having a pale spot. This pale spot varies from barely visible (e.g., G. harveyi sp. nov.), to a large area covering at least a third of the gena height (e.g., G. albigena sp. nov.). This species group contains nine species: G. albigena Fagan-Jeffries, Bird & Austin sp. nov., G. andamookaensis Fagan-Jeffries, Bird & Austin sp. nov., G. aspersus Fagan-Jeffries, Bird & Austin sp. nov., G. austrinus Fagan-Jeffries, Bird & Austin sp. nov. sp. nov., G. ferrugineus Fagan-Jeffries, Bird & Austin sp. nov., G. harveyi Fagan-Jeffries, Bird & Austin sp. nov., G. kittelae Fagan-Jeffries, Bird & Austin sp. nov., G. mnesampela Austin, 2000 and G. sanniopolus Fagan-Jeffries, Bird & Austin sp. nov. The species group, as currently known, has a broad distribution across the southern half of Australia (Fig. 4A)., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on page 17, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles afiamaluanus (Fullaway, 1941) Fig. 16A���B Diagnosis Glyptapanteles afiamaluanus is easily separated from any of the described species of Glyptapanteles in the Australasian region by the distinctive shape of T1, which broadens significantly in the anterior half and then narrows posteriorly and the strong median carina on the propodeum. Material examined No specimens examined, illustrations in Austin & Dangerfield (1992: figs 49���50) used as reference (Fig. 16A���B). Remarks Host unknown. This species is very distinctive amongst the Australasian fauna and the genus placement is very tentative. Distribution Samoa (Upolu Is)., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on page 20, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052, {"references":["Austin A. D. & Dangerfield P. C. 1992. Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics 6: 1 - 76. https: // doi. org / 10.1071 / IT 9920001"]}

Glyptapanteles lessardi Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 3DDE9CAF-3AD2-49C2-BBD6-875FF512E6C9 Fig. 43 Diagnosis Glyptapanteles lessardi sp. nov. is in the G. arcanus species group and can be separated from the other members of the group as follows: Glyptapanteles lessardi sp. nov. can be separated from G. rodriguezae sp. nov. and G. ruhri sp. nov. by T1 being smooth and shiny, not having punctures that cover at least a third of the area of the posterior half of the tergite. Glyptapanteles lessardi sp. nov. can be separated from G. goodwinnoakes sp. nov., G. erucadesolator sp. nov., G. lambkinae sp. nov., G. arcanus sp. nov. and G. vergrandiacus sp. nov. by the propodeum being less coarsely (and less consistently), strongly rugose sculptured across the anterior half of the propodeum. Glyptapanteles wrightae sp. nov. has the propodeum with strong punctures in the anterior half, the posterior half with shallow or strong rugose sculpturing. Glyptapanteles lessardi sp. nov. can be separated from G. doreyi sp. nov. by having the tegula pale in colouration (the tegula in G. doreyi sp. nov. is generally dark in colouration), the hind femur mostly pale or light brown (mostly dark in G. doreyi sp. nov.) and the indentation in the centre of the mesopleuron being smooth, not strongly canaliculated as it is in G. doreyi sp. nov. There was not a morphological character found that easily separates G. lessardi sp. nov. from G. wrightae sp. nov.; we do not diagnose these species morphologically. The wingless barcodes of the two species differ by 3 bp and the COI is greater than 4% divergent. Etymology Named for Dr Bryan Lessard, part of the team who collected the holotype, for his constant endeavour to raise awareness about the importance of taxonomy in the community and his support of other early career researchers. Material examined Holotype AUSTRALIA ��� ♀; Queensland, Blue Tongue Scrub, Steve Irwin Reserve, Cape York; -12.286584, 142.343274; 42.9 m a.s.l.; 23���26 Jul. 2018; C. Lambkin and B. Lessard leg.; vine thicket rainforest; Extraction888, BOLD: AUGLY015-21; QM T250974. Paratype AUSTRALIA ��� 1 ♀; same collection data as for preceding; Extraction890, BOLD: AUGLY016-21; QM T250975. Description Female COLOURATION. Gena without a pale spot; labrum mostly dark; scape colour in ventral half uniformly paler than flagellomeres; flagellomeres uniformly reddish-brown; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum dark with significant orange patches on posterolateral corners or dark with very slight orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; hind coxa dark; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark reddish-brown; T2 sclerotised area dark reddish-brown; T2 lateral area dark extends past indentation, but then pale; T3 uniformly brown; T4+ reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 1.9 mm; fore wing length 1.8 mm; antennal length slightly longer than body length. HEAD. Antennal flagellomere 14 length/width 1.14���1.28; antennal flagellomere 2 length/width 3.00��� 3.16; OOD/POD 2.17���2.20; IOD/POD 1.80���1.83. MESOSOMA. Anteromesoscutum sculpturing with shallow to deep punctures, space between punctures a mixture of smaller than diameter of punctures and of similar size, often smoother in posterior centre; scutellar disk sculpturing with shallow punctures scattered over most of area; seven pits in scutellar sulcus; propodeum with median carina absent and with punctured sculpturing in ���v��� shape in centre of propodeum, or with shallow sculpturing over most of propodeum other than smoother area in anterior centre. WINGS. Pterostigma length 0.46 mm; pterostigma width 0.15 mm; r 0.15 mm; 2RS 0.12 mm; 2m 0.09 mm; (RS+M)b 0.05 mm. METASOMA. T1 lateral edges parallel for anterior �� to ⅔ of length, then narrowing posteriorly; T1 smooth and shiny; T1 length 0.32 mm; T1 width at posterior edge 0.09 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.12 mm; T2 width at posterior edge 0.3 mm; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks Glyptapanteles lessardi sp. nov. constitutes BIN BOLD:AEI3823 and is 4.49% (p-dist.) divergent from the closet BIN in the database (BOLD:AEI5416, an undescribed lineage from Australia, possibly part of G. wrightae sp. nov. (see details under G. wrightae sp. nov. Remarks section). Using the BOLD Batch ID engine, the COI sequence of the holotype is 4.8% different from the most similar COI sequence from an Australian specimen (AUMIC084-18; Glyptapanteles wrightae sp. nov.). Distribution Currently known only from a single reserve in Cape York, the most northern region of QLD., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 85-86, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Genus Glyptapanteles Ashmead, 1904 Glyptapanteles Ashmead, 1904: 147. Glyptapanteles ��� Mason 1981: 105. ��� Austin & Dangerfield 1992: 32. ��� Arias-Penna et al. 2019 a: 561. For complete list of earlier synonyms and bibliography, see Shenefelt (1972) and Fern��ndez- Triana et al. (2020). Type species By monotypy, Apanteles ashmeadi Wilkinson, 1928: 84, a replacement name for Glyptapanteles manilae Ashmead, 1904. Diagnosis The diagnosis by Fern��ndez-Triana et al. (2020) works well for the Australian fauna and is as follows: ������fore wing without an areolet; propodeum that is either completely smooth (often) to more or less rugose (more rarely), with a median longitudinal carina that is entirely absent (often), partially defined posteriorly (often) to complete and strong (rarely), or no median carina but instead a series of very short carinae radiating from the nucha (rarely); T1 narrows towards the posterior margin, usually strongly (almost always), or more parallel-sided, or rounded at apex, as in some species of Protapanteles (rarely); T2 is almost always subtriangular or trapezoidal (rarely shaped differently); ovipositor and ovipositor sheaths are relatively short (usually) to moderately long (rarely); setae at apex of ovipositor sheaths relatively long (as long or longer than setae on hypopygium)��� (Fern��ndez-Triana et al. 2020: 36). Key to the described species groups and to the morphologically distinct species of Glyptapanteles from Australia This key is based on adult females. Due to there likely being many other undescribed species of Glyptapanteles in Australia, it should be treated with caution as undescribed species could possibly key to described species within this key. We feel that a morphological key is not particularly useful for this group, other than for separating the species groups which are quite distinct (e.g., the G. albigena species group, that has the gena with a pale spot), but we present one here for completeness. Ideally, morphological identifications should be supported with COI and wingless DNA barcodes. Species that come out together at a couplet are not reliably distinguished using morphology (e.g., couplet 7). 1. Gena with a pale spot (G. albigena species group) (Fig. 5 A-D)...................................................... 2 ��� Gena without a pale spot (Fig. 5E)................................................................................................... 8 2. Gena with a large pale spot (Fig. 5A���B).......................................................................................... 3 ��� Gena with a small pale spot (e.g., Fig. 5C���D).................................................................................. 4 3. Gena with a pale spot measuring at least one third of face height (Fig. 5A).............................................................................................. Glyptapanteles albigena Fagan-Jeffries, Bird & Austin sp. nov. ��� Gena with a pale spot less than one third of face height (Fig. 5B) ....................................................................................................... Glyptapanteles sanniopolus Fagan-Jeffries, Bird & Austin sp. nov. 4. T2 pale (e.g., Fig. 6A���E)..................................................................... G. mnesampela Austin, 2000 ��� T2 dark (e.g., Fig. 6F)....................................................................................................................... 5 5. Propodeum with median carina faintly indicated posteriorly and anteriorly (Fig. 7A); and gena with a barely visible pale spot (Fig. 5D)....... Glyptapanteles harveyi Fagan-Jeffries, Bird & Austin sp. nov. ��� Propodeum with median carina completely absent (e.g., Fig. 7B); gena with a clearly visible pale spot (e.g., Fig. 5C)............................................................................................................................ 6 6. Hind femur mostly dark (sometimes with lighter area proximally) (Fig. 8A)................................... ���������............................... Glyptapanteles andamookaensis Fagan-Jeffries, Bird & Austin sp. nov. ��� Hind femur mostly light brown or pale (e.g., Fig. 8B)..................................................................... 7 7. Mesosoma with strong red tinge (Fig. 9A)............................................................................................................................................ Glyptapanteles ferrugineus Fagan-Jeffries, Bird & Austin sp. nov. ��� Mesosoma dark, no strong red tinge (e.g., Fig. 9B).................................................................................................................................... Glyptapanteles kittelae Fagan-Jeffries, Bird & Austin sp. nov., Glyptapanteles austrinus Fagan-Jeffries, Bird & Austin sp. nov. and Glyptapanteles aspersus Fagan-Jeffries, Bird & Austin sp. nov. These species cannot be reliably identified using morphology alone; see under the relevant species for molecular information which can be used to differentiate them 8. T2 pale (e.g., Fig. 6A���E).................................................................................................................. 9 ��� T2 dark (e.g., Fig. 6F)......................................................................................................................11 9. T1 same colour as T2, extremely pale (Fig. 6B); propodeum with median carina clear and complete; hind coxa pale (G. eburneus species group, part)...................................................................................................................................... Glyptapanteles eburneus Fagan-Jeffries, Bird & Austin sp. nov. ��� T1 darker than T2, either dark or orange-brown (e.g., Fig. 6C���D); propodeum with median carina absent; hind coxa dark (G. mouldsi species group)........................................................................ 10 10. T1 dark, S3���5 mostly pale or yellow other than hypopygium (which is dark); T3���5 mostly pale or yellow (Fig. 11A���D)................ Glyptapanteles mouldsi Fagan-Jeffries, Bird & Austin sp. nov. and Glyptapanteles dowtoni Fagan-Jeffries, Bird & Austin sp. nov. These species cannot be reliably identified using morphology alone; see under the relevant species for molecular information which can be used to differentiate them ��� T1 orange to light brown, S5���7 dark (including hypopygium); T4���6 completely dark (Fig. 11E���F)................................................................ Glyptapanteles rixi Fagan-Jeffries, Bird & Austin sp. nov. 11. T1 pale; hind coxa pale (G. eburneus species group, part)................................................................................................................... Glyptapanteles foraminous Fagan-Jeffries, Bird & Austin sp. nov. ��� T1 dark; hind coxa dark.................................................................................................................. 12 12. Anteromesoscutum with very sparse deep punctures, always with some smooth areas greater than diameter of punctures; T1 never wedge-shaped, propodeum coarsely rugose; sometimes with slight orange markings on postero-lateral anteromesoscutum (G. niveus species group) (e.g., Fig. 12A��� B).................................................................................................................................................. 13 ��� Anteromesoscutum normally with punctures denser than in Fig. 12A���B, smooth areas generally smaller than diameter of punctures; T1 variable, including sometimes wedge-shaped, propodeum variable, coarsely rugose to smooth; sometimes with strong orange markings on postero-lateral anteromesoscutum (e.g., Fig. 12C���D)............................................................................................ 14 13. Hind coxa with strong punctures covering most of area; antennal flagellomeres all dark..................................................................... Glyptapanteles bradfordae Fagan-Jeffries, Bird & Austin sp. nov. ��� Hind coxa with only sparse punctures, particularly smooth anteriorly; antennal flagellomeres sometimes with white distal segments................................................................................................................................................... Glyptapanteles cooperi Fagan-Jeffries, Bird & Austin sp. nov. and Glyptapanteles niveus Fagan-Jeffries, Bird & Austin sp. nov. These species cannot be reliably identified using morphology alone; see under the relevant species for molecular information which can be used to differentiate them 14. Postero-lateral anteromesoscutum with strong orange markings (Fig. 12C) [whole body strongly punctured, propodeum strongly rugose]...................................................................................................................................................... Glyptapanteles baylessi Fagan-Jeffries, Bird & Austin sp. nov. ��� Postero-lateral anteromesoscutum without strong orange markings (e.g., Fig. 12D) [sculpturing of propodeum variable, sometimes strongly rugose].......................................................................... 15 15. Ventral side of antennal scape (at least in distal half) darker than or the same colour as flagellomeres (e.g., Fig. 13A)................................................................................................................................ 16 ��� Ventral side of antennal scape (at least in distal half) paler than flagellomeres (G. arcanus species group) (e.g., Fig. 13B).................................................................................................................... 20 16. Fore wing veins r and 2RS narrow and long, each significantly longer than vein 2m and smoothly curved (not meeting at sharp angle) (Fig. 14A).................................................................................................................................. Glyptapanteles kurandaensis Fagan-Jeffries, Bird & Austin sp. nov. ��� Fore wing veins r and 2RS each only slightly longer than vein 2m (Fig. 14B), sometimes meeting at sharp angle...................................................................................................................................... 17 17. Tegula dark........................................................................... G. deliasa Austin & Dangerfield, 1992 ��� Tegula pale....................................................................................................................................... 18 18. Maximum height of mesoscutellum lunules less than 0.64�� maximum height of lateral face of mesoscutellum (Fig. 15A) [hind femur darkening posteriorly, propodeum smooth]....................................................................................... Glyptapanteles drioplanetus Fagan-Jeffries & Austin, 2021 ��� Maximum height of mesoscutellum lunules approximately 0.75�� maximum height of lateral face of mesoscutellum (e.g., Fig. 15B) [hind femur completely dark or mostly pale yellow with dark patch posteriorly, propodeum smooth or with some shallow indistinct sculpturing in centre] (G. austini species group)................................................................................................................................. 19 19. Hind femur mostly pale with dark area posteriorly [propodeum smooth and shiny]................................................................................... Glyptapanteles guzikae Fagan-Jeffries, Bird & Austin sp. nov. ��� Hind femur completely dark [propodeum smooth or with some sculpturing in centre]........................................................................ Glyptapanteles kingae Fagan-Jeffries, Bird & Austin sp. nov. and Glyptapanteles austini Fagan-Jeffries & Bird sp. nov. These species cannot be reliably identified using morphology alone; see under the relevant species for molecular information which can be used to differentiate them 20. Propodeum with coarse rugose sculpturing over most of area....................................................... 21 ��� Propodeum smooth, or with rugose sculpturing only in posterior half of centre, anterior half punctured or smooth........................................................................................................................................ 22 21. T1 smooth........................... Glyptapanteles goodwinnoakes Fagan-Jeffries, Bird & Austin sp. nov. ��� T1 with strong or shallow rugose sculpturing........................................................................................................................................ Glyptapanteles lambkinae Fagan-Jeffries, Bird & Austin sp. nov., Glyptapanteles arcanus Fagan-Jeffries, Bird & Austin sp. nov., Glyptapanteles vergrandiacus Fagan-Jeffries, Bird & Austin sp. nov. and Glyptapanteles erucadesolator Fagan-Jeffries, Bird & Austin sp. nov. These species cannot be reliably identified using morphology alone; see under the relevant species for molecular information which can be used to differentiate them 22. T1 with strong punctures in posterior half, punctures covering at least one third of posterior half of tergite................................. Glyptapanteles rodriguezae Fagan-Jeffries, Bird & Austin sp. nov. and Glyptapanteles ruhri Fagan-Jeffries, Bird & Austin sp. nov. These species cannot be reliably identified using morphology alone; see under the relevant species for molecular information which can be used to differentiate them ��� T1 smooth and shiny, if punctures present then only very few and very sparse or confined to lateral edges, covering much less than one third of area of posterior half of tergite............................................................................................ Glyptapanteles wrightae Fagan-Jeffries, Bird & Austin sp. nov., Glyptapanteles doreyi Fagan-Jeffries, Bird & Austin sp. nov. and Glyptapanteles lessardi Fagan-Jeffries, Bird & Austin sp. nov. These species cannot be reliably identified using morphology alone; see under the relevant species for molecular information which can be used to differentiate them Species groups assignments and diagnoses Six species groups are designated based on a combination of molecular and morphological data.Diagnoses for these groups are given below and all of the treated species are then presented in alphabetical order., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 9-17, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052, {"references":["Mason W. R. M. 1981. The polyphyletic nature of Apanteles Foerster (Hymenopera: Braconidae): a phylogeny and reclassification of Microgastrinae. Memoirs of the Entomological Society of Canada 113: 1 - 147. https: // doi. org / 10.4039 / entm 113115 fv","Austin A. D. & Dangerfield P. C. 1992. Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics 6: 1 - 76. https: // doi. org / 10.1071 / IT 9920001","Arias-Penna D. C., Whitfield J. B., Janzen D. H., Hallwachs W., Dyer L. A., Smith M. A., Hebert P. D. N. & Fernandez-Triana J. L. 2019. A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador. ZooKeys 890: 1 - 685. https: // doi. org / 10.3897 / zookeys. 890.35786","Shenefelt R. D. 1972. Braconidae 4, Microgasterinae, Apanteles. In: van der Vecht J. & Shenefelt R. D. (eds) Hymenopterorum Catalogus Part 7: 429 - 668. W. Junk. Gravenhage.","Wilkinson D. S. 1928. A revision of the Indo-Australian species of the genus Apanteles (Hym. Bracon.). Part II. Bulletin of Entomological Research 19: 109 - 146. https: // doi. org / 10.1017 / S 0007485300020393","Fernandez-Triana J., Shaw M. R., Boudreault C., Beaudin M. & Broad G. R. 2020. Annotated and illustrated world checklist of Microgastrinae parasitoid wasps (Hymenoptera, Braconidae). ZooKeys 920: 1 - 1089. https: // doi. org / 10.3897 / zookeys. 920.39128","Fagan-Jeffries E. P. & Austin A. D. 2021. Four new species of parasitoid wasp (Hymenoptera: Braconidae) described through a citizen science partnership with schools in regional South Australia. Zootaxa 4949 (1): 79 - 101. https: // doi. org / https: // doi. org / 10.11646 / zootaxa. 4949.1.4"]}

Glyptapanteles kurandaensis Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 14DC77FB-9FEB-4DE4-8D02-4678EBB481E5 Figs 12D, 14A, 41 Diagnosis Glyptapanteles kurandaensis sp. nov. can be separated from the other species of Glyptapanteles described from Australia by having the gena without a pale spot, T1 and T2 dark, anteromesoscutum punctures not extremely sparse and deep (i.e., not as in the G. niveus species group), no orange markings on the postero-lateral anteromesoscutum, propodeum without strong rugose sculpturing (sometimes with shallow sculpturing in centre, but never over most of propodeum), hind femur solidly dark in colouration, ventral side of antennal scape (at least in distal half) the same colour or darker than the flagellomeres, and fore wing veins r and 2SR narrow and smoothly joined, both significantly longer than vein 2m (compared to the sharply angled, thicker and shorter r and 2SR veins more typical of this genus in Australia). Etymology Named for the collection locality, Kuranda, a small town near Cairns in northern QLD. Material examined Holotype AUSTRALIA ��� ♀; Queensland, Kuranda; -16.8135; 145.6430586; 317 m a.s.l.; 12 Feb.���6 Apr. 2020; M.S. Moulds leg.; Malaise Trap EFJ2020MT36; Extraction1568, BOLD: AUGLY110-21; QM T250958. Paratypes AUSTRALIA ��� Queensland ��� 1 ♀; same collection data as for holotype: Extraction1520, BOLD: AUGLY092-21; QM T250959 ��� 1 ♀; same collection data as for holotype; Extraction1542, BOLD: AUGLY098-21; QM T250960 ��� 1 ♀; same collection data as for holotype; Extraction1543, BOLD: AUGLY099-21; QM T250961 ��� 1 ♀ (ethanol); same collection data as for holotype; Extraction1546, BOLD: AUGLY101-21; QM T250962 ��� 1 ♀ (ethanol); same collection data as for holotype; Extraction1551, BOLD: AUGLY103-21; QM T250963 ��� 1 ♀; same collection data as for holotype; Extraction1554, BOLD: AUGLY104-21; QM T250964 ��� 1 ♀ (ethanol); same collection data as for holotype; Extraction1565, BOLD: AUGLY109-21; QM T250965 ��� 1 ♀; same collection data as for holotype; Extraction1569, BOLD: AUGLY111-21; QM T250966 ��� 1 ♀; same collection data as for holotype; Extraction1570, BOLD: AUGLY112-21; QM T250967 ��� 1 ♀; same collection data as for holotype; Extraction1508, BOLD: AUGLY129-21; QM T250968 ��� 1 ♀; same collection data as for holotype; Extraction296, BOLD: AUMIC161-18; QM T250969 ��� 1 ♀ (ethanol); same collection data as for holotype; 10 Feb.���15 Mar. 2017; Extraction500, BOLD: AUMIC306-18; QM T250970 ��� 1 ♀; same collection data as for holotype; 16 Mar.���12 Apr. 2017; Extraction551, BOLD: AUMIC342-18; QM T250971. Description Female COLOURATION. Gena without a pale spot; labrum mostly dark or reddish-brown; scape colour in ventral half the same colour or darker than flagellomeres; flagellomeres darkening distally; tegula dark; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark; scutellar disk and metanotum dark; propodeum dark; fore coxa dark; mid coxa dark; hind coxa dark; fore femur pale yellow; mid femur pale yellow; hind femur dark reddish-brown or dark; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark; T2 sclerotised area dark or dark reddish-brown; T2 lateral area same colour as sclerotised area, or only slightly paler, or dark extends past indentation, but then pale; T3 dark, mostly dark with paler lateral areas or uniformly brown; T4+ dark or reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 1.8 mm; fore wing length 1.9 mm; antennal length slightly shorter than body length. HEAD. Antennal flagellomere 14 length/width 1.80���2.25; antennal flagellomere 2 length/width 2.42��� 3.80; OOD/POD 1.86���2.33; IOD/POD 1.29���2.00. MESOSOMA. Anteromesoscutum sculpturing with shallow punctures, space between punctures generally smaller than diameter of punctures, slightly denser and deeper punctures anteriorly, smoother in posterior centre; scutellar disk sculpturing with only very shallow punctures; 7���9 pits in scutellar sulcus; propodeum with median carina absent, some indistinct sculpturing in posterior centre, or in centre along length of propodeum, or median carina faintly indicated for all of length by indistinct sculpturing. WINGS. Pterostigma length 0.48 mm; pterostigma width 0.15 mm; r 0.17 mm; 2RS 0.12 mm; 2m 0.09 mm; (RS+M)b 0.1 mm. METASOMA. T1 lateral edges parallel for anterior �� of length, then narrowing posteriorly, or lateral edges parallel for entirety of length, posterior corners sometimes rounded at boundary with T2; T1 mostly smooth, some punctures in posterior half or mostly smooth, some shallow rugose sculpturing in posterior half; T1 length 0.29 mm; T1 width at posterior edge 0.14 mm; T2 an isosceles trapezoid, lateral edges straight, with curved lateral and anterior edges, becoming arch- or semicircle-shaped or almost square, lateral edges almost parallel, only broadening posteriorly very slightly; T2 smooth and shiny; T2 length 0.13 mm; T2 width at posterior edge 0.24 mm; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks Glyptapanteles kurandaensis sp. nov. constitutes BIN BOLD:ADL2798 and is 2.4% (p-dist.) divergent from the closet BIN in the database (BOLD:AAH1268, Glyptapanteles creatonoti (Viereck, 1912)). We have examined the images of G. creatonoti in Gupta et al. (2016) and are confident that G. kurandaensis sp. nov. is a different species, most easily separated by the following morphological characteristics: G. kurandaensis sp. nov. has the hind coxa dark (G. creatonoti hind coxa pale; ���stamineous��� in original description); G. kurandaensis sp. nov. has the propodeum much smoother than that of G. creatonoti and G. kurandaensis sp. nov. has T1 completely black, whilst G. creatonoti has the anterior area of T1 pale (���stamineous��� in original description, appearing pale orange in images of Gupta et al. (2016)). Using the BOLD Batch ID engine, the COI barcode of the holotype is 7.7% different from the most similar COI sequence from an Australian specimen (AUMIC291-18; Glyptapanteles austini sp. nov.). The type specimen was sequenced for the wingless gene, which differs by a minimum of 14 bp from all other species with available sequence data. Distribution This species is currently known from one collection site; Kuranda in north-eastern QLD., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 79-82, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052, {"references":["Gupta A., Venkatesan T. & More R. P. 2016. Morphological and molecular characterization of reared parasitoid wasps of the genus Glyptapanteles Ashmead 1904 (Insecta: Hymenoptera: Braconidae: Microgastrinae) associated with Lepidoptera in India. PLoS ONE 11: e 0150765. https: // doi. org / 10.1371 / journal. pone. 0150765"]}

Glyptapanteles deliasa Austin & Dangerfield, 1992 Figs 13A, 27 Diagnosis Glyptapanteles deliasa can be separated from the other described species of Glyptapanteles in Australia by the combination of the following characters: gena without a pale spot, T1 and T2 dark, anteromesoscutum very smooth, with only shallow and regular punctures, no orange markings on the postero-lateral anteromesoscutum, propodeum very smooth with only shallow punctures and the median carina faintly indicated, T1 parallel-sided for most of length, only narrowing/curving inwards right at boundary with T3, ventral side of antennal scape (at least in distal half) darker than flagellomeres, tegula dark. Material examined Paratypes AUSTRALIA ��� 2 ♀♀; South Australia, Waikerie; 22 Jun. 1988; M.D. Moore leg.; ex Delias aganippe (Donovan) (Pieridae); WINC. Remarks There is a possibility that this species is conspecific with Cotesia deliadis (Bingham, 1906), as it is morphologically very similar and parasitises the same host. However, several attempts to extract DNA from paratype specimens to either confirm or refute this hypothesis have been unsuccessful. As such, we leave it within the genus Glyptapanteles until next-generation sequencing techniques can be used on the type series. Distribution Known from Australia, currently only from SA., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on page 46, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052, {"references":["Austin A. D. & Dangerfield P. C. 1992. Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics 6: 1 - 76. https: // doi. org / 10.1071 / IT 9920001"]}

Glyptapanteles cooperi Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 8E2A7ED6-8383-43C1-B1DA-2712F7B6529C Figs 12B, 26 Diagnosis Glyptapanteles cooperi sp. nov. is in the G. niveus species group and can be separated from G. bradfordae sp. nov. by the hind coxa of G. cooperi sp. nov. only being sparsely punctured, particularly on the dorsal area and from G. niveus sp. nov. by the labrum being pale in colour (labrum dark in G. niveus sp. nov.). Etymology Named for Professor Steven Cooper, who has provided extensive mentoring to EPF-J during her PhD. Material examined Holotype AUSTRALIA ��� ♀; Queensland, Kuranda; -16.8154, 145.643; 317 m a.s.l.; 16 Mar.���12 Apr. 2017; M.S. Moulds leg.; Malaise Trap; Extraction548, BOLD: AUMIC340-18; QM T250947. Description Female COLOURATION. Gena without a pale spot; labrum pale; scape colour in ventral half paler than flagellomeres at proximal end; flagellomeres all black/dark brown; tegula dark; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum dark with very slight orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; fore coxa white; mid coxa white; hind coxa dark; hind femur orange to light brown; hind tibia light brown; hind basitarsus light brown; T1 dark; T2 sclerotised area dark; T2 lateral area same colour as sclerotised area, or only slightly paler; T3 mostly dark with paler lateral areas; T4+ dark. HOLOTYPE BODY MEASUREMENTS. Body length 2.3 mm; fore wing length 2.2 mm. HEAD. Face densely sculptured, punctate reticulate; antennal flagellomere 2 length/width 3.63; OOD/ POD 1.50; IOD/POD 1.50. MESOSOMA. Anteromesoscutum with very sparse, deep punctures; scutellar disk smooth in centre, some deep punctures on lateral edges; six pits in scutellar sulcus; propodeum with median carina present and complete, rest of propodeum strongly rugose. WINGS. Pterostigma length 0.58 mm; pterostigma width 0.17 mm; r 0.15 mm; 2RS 0.1 mm; 2m 0.11 mm; (RS+M)b 0.08 mm. METASOMA. T1 lateral edges parallel for anterior 1/4 of length, then gently narrowing posteriorly, lateral edges often with slight curve; T1 smooth in anterior half, punctate reticulate in posterior half; T1 length 0.5 mm; T1 width at posterior edge 0.21 mm; T2 width greater than length, rectangular with rounded anterior corners; T2 smooth and shiny; T2 length 0.15 mm; T2 width at posterior edge 0.36 mm; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks Glyptapanteles cooperi sp. nov. constitutes BIN: BOLD:ADL4822 and is 6.98% (p-dist.) divergent from the closet BIN in the database (BOLD:ADG5990, an undescribed lineage from Australia, with two specimens). Using the BOLD Batch ID engine, the COI barcode of the holotype is 6.8% different from the most similar COI sequence from an Australian specimen (GMQQW352-18; an undescribed lineage, with two specimens). The type specimen was able to be sequenced for the wingless gene, which differs by a minimum of 4 bp from all other species with available sequence data. Distribution This species is currently only represented by a single specimen from Kuranda, northern QLD., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 44-46, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles bradfordae Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 6C1A43F3-D6F0-4FD3-A100-F83B4EB894E8 Figs 12A, 25 Diagnosis Glyptapanteles bradfordae sp. nov. is in the G. niveus species group and can be differentiated from G. niveus sp. nov. by the absence of white distal flagellomeres, a pale labrum and denser punctures on the hind coxa. Glyptapanteles bradfordae sp. nov. can be separated from G. cooperi sp. nov. by having denser punctures on the hind coxa, particularly in the dorsal area. Etymology This species is named for Dr Tessa Bradford, Facility Manager of the South Australian Regional Facility for Molecular Ecology and Evolution, without whom the DNA sequencing of many of these specimens would not have occurred. The authors thank Tessa for her continual advice, support and training in the molecular labs! Material examined Holotype AUSTRALIA ��� ♀; Queensland, Kuranda; -16.8135, 145.6430586; 317 m a.s.l.; 14 Dec. 2019 ��� 6 Jan. 2020; M.S. Moulds leg.; Malaise Trap EFJ2020MT11; Extraction1096, BOLD: AUGLY046-21; QM T250939. Paratypes AUSTRALIA ��� 1 ♂ (ethanol); same collection data as for holotype; 19 May���8 Jul. 2017; Extraction1295, BOLD: AUGLY078-21; QM T250940 ��� 1 ♂; same collection data as for holotype; Extraction1297, BOLD: AUGLY079-21; QM T250941 ��� 1 ♂ (ethanol); same collection data as for holotype; 12 Feb.���6 Apr. 2020; EFJ2020MT36; Extraction1524, BOLD: AUGLY094-21; QM T250942 ��� 1 ♂ (ethanol); same collection data as for holotype; 1���21 Jan. 2017; Extraction292, BOLD: AUMIC157-18; QM T250943 ��� 1 ♀; same collection data as for holotype; Extraction290, BOLD: AUMIC155-18; QM T250944 ��� 1 ♀; same collection data as for holotype; 12 Feb.���6 Apr. 2020; Extraction1544, BOLD: AUGLY100-21; QM T250945 ��� 1 ♂; Lamington National Park; -28.207, 153.137; 560 m a.s.l.; 9���19 Mar. 2007; C.7 Lambkin and N. Starick leg.; IBISCA Plot # IQ-500-A rainforest Malaise trap, bulk vial 22028; Extraction1461, BOLD: AUGLY081-21; QM T250946 ��� 1 ♀; same collection data as for preceding; Extraction633, BOLD: AUMIC417-18; QM T208409. Description Female COLOURATION. Gena without a pale spot; labrum pale; scape colour in ventral half uniformly paler than flagellomeres or the same colour or darker than flagellomeres; flagellomeres all black/dark brown or uniformly reddish-brown; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum dark with significant orange patches on posterolateral corners; scutellar disk and metanotum dark or dark with red tinge; propodeum dark; fore coxa white; mid coxa white; hind coxa dark; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark or dark reddish-brown; T2 sclerotised area dark or dark reddish-brown; T2 lateral area much paler; T3 dark or mostly dark with paler lateral areas; T4+ dark or reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 1.8 mm; fore wing length 2.3 mm; antennal length slightly longer than body length. HEAD. Face densely sculptured, punctate reticulate; antennal flagellomere 14 length/width 1.85���2.00; antennal flagellomere 2 length/width 3.63���4.50; OOD/POD 1.33���1.71; IOD/POD 1.33���1.71. Eyes particularly large, with a smaller malar space than most species of Glyptapanteles. MESOSOMA. Anteromesoscutum sculpturing with shallow punctures, space between punctures generally smaller than diameter of punctures, slightly denser and deeper punctures anteriorly, smoother in posterior centre or very sparse, deep punctures; scutellar disk sculpturing smooth in centre, with some deep punctures on lateral edges; six pits in scutellar sulcus; propodeum with median carina very faintly indicated at either anterior or both posterior and anterior edges, propodeum strongly rugose. WINGS. Pterostigma length 0.57 mm; pterostigma width 0.18 mm; r 0.2 mm; 2RS 0.1 mm; 2m 0.12 mm; (RS+M)b 0.09 mm. METASOMA. T1 lateral edges parallel for anterior �� of length, then narrowing posteriorly or broadest at centre of length, narrowing both posteriorly and anteriorly from mid-point; T1 smooth in anterior half, punctate reticulate to rugose in posterior half; T1 length 0.38 mm; T1 width at posterior edge 0.13 mm; T2 an isosceles trapezoid, lateral edges straight or almost square, lateral edges almost parallel, only broadening posteriorly very slightly; T2 with some shallow indistinct sculpturing; T2 length 0.12 mm; T2 width at posterior edge 0.22 mm; ovipositor slightly protruding from end of metasoma. Male As female, 6���7 pits in scutellar sulcus. Remarks Glyptapanteles bradfordae sp. nov. constitutes BIN BOLD:AEI7940 and is 2.01% (p-dist.) divergent from the closet BIN in the database (BOLD:ADD8337, with three specimens from Papua New Guinea, see below). Using the BOLD Batch ID engine, the COI barcode of the holotype is 7.6% different from the most similar COI sequence from an Australian specimen (GMAQJ131-17; an undescribed lineage, with two specimens). Eight specimens were able to be sequenced for the wingless gene, which all share a unique barcode that differs by a minimum of 3 bp from all other species with available sequence data. There are three additional specimens sequenced for COI on BOLD which fall within 2% pairwise divergence and are in the closely related BIN BOLD:ADD8337. These specimens (GMNGH687-16, GMNGJ510-16 and GMNGS061-16) were all collected in Papua New Guinea, Madang, by Pagi Toko and are most likely the same species. Specimens were not examined, but a dorsal habitus image on BOLD (http://www.boldsystems.org/pics/GMNGS/BIOUG30657-C01%2B1487790120.jpg) supports this suggestion. Distribution This species is currently known from the east coast of QLD, from both Lamington National Park in the south and Kuranda in the north. It is also likely found in Papua New Guinea (based on the presence of closely related COI barcodes on BOLD)., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 41-44, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles eburneus species group The G. eburneus species group currently contains only two closely related species, G. eburneus Fagan- Jeffries, Bird & Austin sp. nov. and G. foraminous Fagan-Jeffries, Bird & Austin sp. nov. which are differentiated from all other species of Glyptapanteles in Australia by having the gena without a pale spot, T1 completely pale, hind coxa pale, deep punctures on the anteromesoscutum and a clear median carina on the propodeum. This species group is known from two single localities on the east coast of Australia, in QLD and NSW (Fig. 4D).

Glyptapanteles mouldsi species group The G. mouldsi species group can be distinguished from all of the other Glyptapanteles described from Australia by having the gena without a pale spot, T2 pale in colouration, with T1 darker than T2 and no median carina on the propodeum. It contains three newly described species: G. dowtoni Fagan-Jeffries, Bird &Austin sp. nov., G. mouldsi Fagan-Jeffries, Bird &Austin sp. nov. and G. rixi Fagan-Jeffries, Bird & Austin sp. nov. The group is paraphyletic in the current phylogeny (Fig. 2). The G. mouldsi species group is currently only known from the east coast of Australia, from northern QLD to central NSW (Fig. 4E).

Glyptapanteles sanniopolus Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: B70D5575-E6F6-496F-8B47-453AD631CC2E Figs 5B, 51 Diagnosis Glyptapanteles sanniopolus sp. nov. is in the G. albigena species group and can be separated from most other members of the species group by having the gena with a large pale spot (Fig. 51G) rather than small (e.g., Fig. 40A) and from G. albigena sp. nov. by having the gena spot covering less than a third of the gena height and more rounded at the dorsal edge. Etymology The species epithet ‘ sanniopolus ’ is a combination of the Latin words ‘sannio’ ('one who makes faces’) and 'polus’ ('pole of the earth’) and refers to the pale spot on the gena characteristic of this species and of the broader G. albigena species group. It is a noun in apposition. Material examined Holotype AUSTRALIA • ♀; New South Wales, Monga National Park near Penance Grove Walk; -35.597372, 149.912126; 9 Nov. 2019 – 16 Jan. 2020; K.M. Bayless and J.G. Lumbers leg.; Malaise trap over stream, trap destroyed by bushfire 30 Dec. 2019, partially dried out; Extraction1666, BOLD: AUGLY127-21; ANIC 32 130370. Paratypes AUSTRALIA – New South Wales • 1 ♂; same collection data as for preceding; Extraction1638, BOLD: AUGLY121-21; ANIC 32 130371. – Tasmania • 1 ♂; Hobart, Kingston Beach; -42.986, 147.317; 14– 18 Sep. 2010; B. Ward leg.; BIOUG00996-D09, BOLD: HYAT425-11; ANIC 32 130372. Description Female COLOURATION. Gena with a pale spot; labrum pale; scape colour in ventral half the same colour or darker than flagellomeres; flagellomeres all black/dark brown; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark or dark with very slight orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; fore coxa pale yellow; mid coxa pale yellow; hind coxa dark; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow or light brown; mid tibia light brown; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark; T2 sclerotised area dark or dark reddish-brown; T2 lateral area same colour as sclerotised area, or only slightly paler, or dark extends past indentation, but then pale; T3 dark or uniformly brown; T4+ dark or reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 2.4 mm; fore wing length 2.6 mm; antennal length slightly longer than body length. HEAD. Antennal flagellomere 14 length/width 2.6; antennal flagellomere 2 length/width 4.33; OOD/ POD 1.86; IOD/POD 1.43. MESOSOMA. Anteromesoscutum sculpturing with shallow to deep punctures, space between punctures a mixture of smaller than diameter of punctures and of similar size. Often smoother in posterior centre; scutellar disk sculpturing with only very shallow punctures, smooth and shiny; nine pits in scutellar sulcus; propodeum with median carina present and complete, rest of propodeum strongly rugose. WINGS. Pterostigma length 0.6 mm; pterostigma width 0.23 mm; r 0.16 mm; 2RS 0.15 mm; 2m 0.13 mm; (RS+M)b 0.1 mm. METASOMA. T1 wedge-shaped, narrowing posteriorly for entirety of length, lateral edges straight (but not parallel); T1 smooth and shiny, some shallow scattered punctures on lateral edges and posterior half or smooth in anterior half, indistinct sculpturing in posterior half; T1 length 0.62 mm; T1 width at posterior edge 0.1 mm; T2 an isosceles trapezoid, lateral edges straight; T2 with some shallow indistinct sculpturing or smooth and shiny, some shallow punctures along posterior edge; T2 length 0.16 mm; T2 width at posterior edge 0.27 mm; ovipositor slightly protruding from end of metasoma. Male As female, 7–9 pits in scutellar sulcus. Remarks Glyptapanteles sanniopolus sp. nov. constitutes BIN: BOLD:ABA6208 and is 5.48% (p-dist.) divergent from the closet BIN in the database (BOLD:ADL3908; Glyptapanteles albigena sp. nov.). Using the BOLD Batch ID engine, the COI barcode of the holotype is 5.6% different from the most similar COI sequence from an Australian specimen (AUMIC394-18; Glyptapanteles albigena sp. nov.). The two specimens able to be sequenced for the wingless gene share a unique barcode, which differs by a minimum of 3 bp from all other species with available sequence data. Distribution This species is known from southern NSW and TAS.

Glyptapanteles doreyi Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 34246DE7-8966-4FBC-8D6A-ECC5DADF79BC Figs 28���29 Diagnosis Glyptapanteles doreyi sp. nov. is in the G. arcanus species group and can be separated from the other members of the species group as follows: Glyptapanteles doreyi sp. nov. can be separated from G. rodriguezae sp. nov. and G. ruhri sp. nov. by T1 being smooth and shiny, not having punctures that cover at least a third of the area of the posterior half of the tergite. Glyptapanteles doreyi sp. nov. can be separated from G. goodwinnoakes sp. nov., G. erucadesolator sp. nov., G. lambkinae sp. nov., G. arcanus sp. nov. and G. vergrandiacus sp. nov. by the propodeum being less coarsely and less consistently, rugose sculptured across the anterior half of the propodeum. Glyptapanteles doreyi sp. nov. has the propodeum with shallow or strong punctures in the anterior half, the posterior half smooth or with shallow or strong rugose sculpturing and sometimes with a smooth area in the centre. Glyptapanteles doreyi sp. nov. can be separated from G. wrightae sp. nov. and G. lessardi sp. nov. by the tegula being dark in colouration, the same colour or only slightly lighter than the mesosoma (the tegula in G. wrightae sp. nov. and G. lessardi sp. nov. is pale) the hind femur mostly dark (pale or light brown in G. wrightae sp. nov. and G. lessardi sp. nov.) and the indentation in the centre of the mesopleuron being strongly canaliculate. The indentation on the mesopleuron of G. wrightae sp. nov. and G. lessardi sp. nov. is smoother, not strongly canaliculate. Etymology Named for James Dorey, who collected the holotype specimen. EPF-J would like to acknowledge James��� collegiate sharing of specimens and his contribution to a successful field trip in northern QLD in 2019 that led to the collection of many important microgastrine specimens. Material examined Holotype AUSTRALIA ��� ♀; New South Wales, Cockburn River Camp; -31.05222, 151.14411; 461 m a.s.l.; 10 Dec. 2019; J.B. Dorey leg.; 19JDEC100, general sweep over Brachychiton flowering species in schlerophyll forest along dry creek bed, sunny and warm ~33��C; Extraction1067, BOLD:AUGLY041-21; AM K.517929. Paratypes AUSTRALIA ��� Australian Capital Territory ��� 1 ♀; CSIRO Black Mountain off Frith Rd; -35.268, 149.1107; 610 m a.s.l.; 9���26 Oct. 2019; K.M. Bayless leg.; dry sclerophyll forest Malaise trap; Extraction1660, BOLD: AUGLY125-21; ANIC 32 130318 ��� 1 ♀; same collection data as for preceding; Extraction1645, BOLD: AUGLY122-21; ANIC 32 130319 ��� 1 ♀; same collection data as for preceding; Extraction1646, BOLD: AUGLY123-21; ANIC 32 130320 ��� 1 ♀ (ethanol); CSIRO Black Mountain, close to Botanic Garden fence; -35.273611, 149.110556; 538 m a.s.l.; 30 Oct.���6 Nov. 2017; T. Pleines and J. Rodriguez leg.; Malaise; Extraction959, BOLD: AUGLY027-21; ANIC 32 130321 ��� 1 ♀; same collection data as for preceding; 23���30 Oct. 2017; Extraction974, BOLD: AUGLY028-21; ANIC 32 130322 ��� 1 ♀ (ethanol); CSIRO Black Mountain; -35.2744, 149.1115; 6 Dec. 2017 ��� 5 Jan. 2018; J. Rodriguez, C. Waichert, K.M. Bayless and T. Pleines leg.; Malaise 2, green dry wash; Extraction976, BOLD: AUGLY029-21; ANIC 32 130323 ��� 1 ♀ (ethanol); same collection data as for preceding; Extraction978, BOLD: AUGLY030-21; ANIC 32 130324 ��� 1 ♀; same collection data as for preceding; Extraction980, BOLD: AUGLY032-21; ANIC 32 130325 ��� 1 ♀ (ethanol); same collection data as for preceding; Extraction981, BOLD: AUGLY033-21; ANIC 32 130326 ��� 1 ♀ (ethanol); same collection data as for preceding; Extraction982, BOLD: AUGLY034-21; ANIC 32 130327 ��� 1 ♀ (ethanol); same collection data as for preceding; Extraction983, BOLD:AUGLY035-21; ANIC 32 130328 ��� 1 ♀ (ethanol); same collection data as for preceding; 6���20 Jan. 2020; Extraction1658, BOLD: AUGLY124-21; ANIC 32 130329 ��� 1 ♀; CSIRO property; -35.275, 149.111; 588 m a.s.l.; 7 Sep. 2011; P. Hebert leg.; Malaise; BIOUG02156-H03, BOLD: MCCAA225-12; ANIC 32 130330. ��� New South Wales ��� 1 ♀ (ethanol); same collection data as for holotype; Extraction1086, BOLD: AUGLY043-21; AM K.383784 ��� 1 ♀ (ethanol); same collection data as for holotype; Extraction1156, BOLD: AUGLY066-21; AM K.383785 ��� 1 ♂; same collection data as for holotype; Extraction1068, BOLD: AUGLY042-21; AM K.517930 ��� 1 ♂; same collection data as for holotype; Extraction1087, BOLD: AUGLY044-21; AM K.517931 ��� 1 ♂; same collection data as for holotype; Extraction1094, BOLD: AUGLY045-21; AM K.517932 ��� 1 ♂; same collection data as for holotype; Extraction1155, BOLD: AUGLY065-21; AM K.517933 ��� 1 ♂ (ethanol); same collection data as for holotype; Extraction1157, BOLD: AUGLY067-21; AM K.383786 ��� 1 ♀; Oxley Wild Rivers National Park, East Kunderang Track; -30.818056, 152.135; 7 Nov. 2015; D.M. Bray leg.; blue pan trap; Extraction1248, BOLD: AUGLY073-21; AM K.517937. ��� Queensland ��� 1 ♀; Lamington National Park; -28.142, 153.133; 248 m a.s.l.; 8���18 Apr. 2007; C. Lambkin and N. Starick leg.; IBISCA Plot # IQ-300-D rainforest Malaise trap; Extraction605, BOLD: AUMIC390-18; QM T208398 ��� 1 ♀; same collection data as for preceding; Extraction604, BOLD: AUMIC389-18; QM T208397 ��� 1 ♀; same collection data as for preceding; Extraction193, BOLD: AUMIC080-18; QM T208393 ��� 1 ♀ (ethanol); same collection data as for preceding; Extraction632, BOLD: AUMIC416-18; QM T208395 ��� 1 ♀ (ethanol); same collection data as for preceding; 23 Sep.���5 Oct. 2014; Extraction623, BOLD: AUMIC409-18; QM T208394 ��� 1 ♀; same collection data as for preceding; 5���22 Oct. 2014; Extraction627, BOLD: AUMIC412-18; QM T208396. Description Female COLOURATION. Gena without a pale spot; labrum mostly dark or reddish-brown; scape colour in ventral half uniformly paler than flagellomeres or paler than flagellomeres at proximal end; flagellomeres all black/dark brown; tegula normally dark (dark in holotype, sometimes paler in paratypes); wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark or dark with very slight orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; fore coxa dark; mid coxa dark; hind coxa dark; fore femur pale yellow; mid femur pale yellow or light brown; hind femur dark reddish-brown, orange to light brown or dark; fore tibia pale yellow; mid tibia pale yellow or light brown; hind tibia darkening posteriorly; hind basitarsus light brown or dark reddish-brown; T1 dark; T2 sclerotised area dark; T2 lateral area same colour as sclerotised area, or only slightly paler or dark extends past indentation, but then pale; T3 dark, mostly dark with paler lateral areas or uniformly brown; T4+ dark or reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 2.4 mm; fore wing length 2.3 mm; antennal length slightly shorter than body length. HEAD. Antennal flagellomere 14 length/width 1.25���2.00; antennal flagellomere 2 length/width 2.00��� 4.20; OOD/POD 1.57���2.50; IOD/POD 1.29���1.83. MESOSOMA. Anteromesoscutum sculpturing with shallow to deep punctures, space between punctures generally smaller than diameter of punctures; scutellar disk sculpturing with only very shallow punctures; 8���12 pits in scutellar sulcus; propodeum with median carina absent, shallow or strong punctures in anterior half, occasionally with small areas of shallow rugosity, posterior half of propodeum smooth or with shallow or strong rugose sculpturing, propodeum sometimes with smooth area in centre. WINGS. Pterostigma length 0.53 mm; pterostigma width 0.17 mm; r 0.15 mm; 2RS 0.14 mm; 2m 0.09 mm; (RS+M)b 0.08 mm. METASOMA. T1 lateral edges parallel for anterior �� to ⅔ of length, then narrowing posteriorly; T1 sometimes shiny, smooth, or mostly smooth with some punctures in posterior half, or smooth in anterior half with indistinct sculpturing in posterior half; T1 length 0.34 mm; T1 width at posterior edge 0.13 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.14 mm; T2 width at posterior edge 0.31 mm; ovipositor slightly protruding from end of metasoma. Male As female but with slightly longer antennae. Remarks Glyptapanteles doreyi sp. nov. has two wingless haplotypes (differing by a single base pair); however, as the COI divergence between specimens with the two different haplotypes is not consistent (i.e., one specimen has a COI sequence that is more closely related to specimens with the alternative wingless haplotype than to others) and the COI divergence among all specimens is not above 2%, we consider all specimens to be one species. One of the wingless haplotypes is shared with the species G. arcanus sp. nov. and G. goodwinnoakes sp. nov. and both barcodes only differ by 1 bp from the species G. vergrandiacus sp. nov. and G. lessardi sp. nov. This group of species is very closely related and would benefit from future, more detailed taxonomic work to ensure that they are not simply divergent populations of the same species. For this species hypothesis, we have made the decision to split this large clade into different species based on the COI barcode sequences being>3% divergent; however, we note that this is a hypothesis and is open to change with future work. In the IQ-TREE analysis of the concatenated COI and wingless alignment, there are two clades within G. doreyi sp. nov. that differ, in their COI sequences, between 2.0% and 2.5%. The first clade (���clade A���) contains seven sequences (AUMIC416-18, AUMIC409-18, AUMIC080-18, AUGLY044-21, AUMIC412-18, AUMIC390-18, AUMIC389-18). Specimen AUGLY044-21 has the same wingless haplotype as the specimens in the second clade within G. doreyi sp. nov. (���clade B���) whilst the other six specimens share a wingless haplotype that differs by one base pair from that of AUGLY044-21. We have made the conservative decision to combine these two clades into a single species due to the COI divergence being borderline of what is often used in Microgastrinae, lack of clear morphological differences and the wingless haplotype of AUGLY044-21 being identical to that of the alternate clade; however, we provide images of a representative of both clades and note that future evidence (particularly host data) may end up splitting this species further. Glyptapanteles doreyi sp. nov. constitutes BIN BOLD:ABY0421 (clade B) and BOLD:AEC8663 (clade A). Using the BOLD Batch ID engine, the COI sequence of the holotype is 2.6% different from the most similar COI sequence from an Australian specimen (MCCAA7675-20; an undescribed lineage). Distribution This species is currently known from eastern NSW and from the ACT., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 48-52, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles harveyi Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 2217AE78-E98A-4283-97B4-033E2175309C Figs 5D, 6F, 7A, 38 Diagnosis Glyptapanteles harveyi sp. nov. is in the G. albigena species group and can be separated all other members of the species group by having the pale spot on the gena barely visible, T2 dark and the propodeum with the median carina faintly indicated at anterior and posterior ends. Etymology Named for Dr Mark Harvey, who collected the type material and has generously run Malaise traps to collect microgastrine material, along with providing advice for many years on the International Code of Zoological Nomenclature to EPF-J. We thank Mark for his dedication to robust taxonomic science and his support of early career researchers. Material examined Holotype AUSTRALIA ��� ♀; Western Australia, Gleneagle State Forest; -32.2711, 116.163; 8���9 Oct. 2005; M.S. Harvey leg.; Malaise trap; Extraction119, BOLD: AUMIC021-18; WAM E109888. Paratype AUSTRALIA ��� 1 ♀; same collection data as for holotype; Extraction413, BOLD: AUMIC246-18; WAM E109889. Description Female COLOURATION. Gena with a pale spot, but only weakly visible; labrum reddish-brown; scape colour in ventral half the same colour or darker than flagellomeres; flagellomeres all black/dark brown; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark; scutellar disk and metanotum dark; propodeum dark; fore coxa dark; mid coxa dark; hind coxa dark; fore femur orange to light brown; mid femur orange to light brown; hind femur orange to light brown; fore tibia orange to light brown; mid tibia orange to light brown; hind tibia darkening posteriorly; hind basitarsus dark reddish-brown; T1 dark; T2 sclerotised area dark; T2 lateral area dark extending past indentation, but then pale; T3 mostly pale with darker patch in centre or uniformly brown; T4+ reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 2.5 mm; fore wing length 2.6 mm; antennal length slightly longer than body length. HEAD. Antennal flagellomere 14 length/width 2.16���2.4; antennal flagellomere 2 length/width 3.28���3.57; OOD/POD 1.86; IOD/POD 1.71���1.86. MESOSOMA. Anteromesoscutum sculpturing with shallow punctures, space between punctures generally smaller than diameter of punctures; scutellar disk sculpturing with only very shallow punctures; seven pits in scutellar sulcus; propodeum with median carina very faintly indicated in posterior half, anterior half with some punctures, postero-lateral corners smooth and shiny. WINGS. Pterostigma length 0.63 mm; pterostigma width 0.26 mm; r 0.14 mm; 2RS 0.1 mm; 2m 0.11 mm; (RS+M)b 0.1 mm. METASOMA. T1 wedge-shaped, narrowing posteriorly for entirety of length, lateral edges straight (but not parallel); T1 mostly smooth, some punctures in posterior half; T1 length 0.37 mm; T1 width at posterior edge 0.07 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth, some very shallow punctures in posterior half; T2 length 0.16 mm; T2 width at posterior edge 0.25 mm; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks Glyptapanteles harveyi sp. nov. constitutes BIN: BOLD:ADL3315 and is 5.33% (p-dist.) divergent from the closet BIN in the database (BOLD:ACL9711; Glyptapanteles sp. from New Zealand). Using the BOLD Batch ID engine, the COI barcode of the holotype is 6.2% different from the most similar COI sequence from an Australian specimen (AUMIC482-18; Glyptapanteles aspersus sp. nov.). The two specimens were sequenced for the wingless gene and share a unique barcode, which differs by a minimum of 3 bp from all other species with available sequence data. Distribution This species is currently known from Gleneagle State Forest near Perth, WA., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 72-74, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles goodwinnoakes Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: C39D8F6A-C62D-4D6C-AC74-BBEBAED50ED5 Fig. 36 Diagnosis Glyptapanteles goodwinnoakes sp. nov. can be separated from the other members of the G. arcanus species group by having the propodeum with generally coarser sculpturing than most species other than G. erucadesolator sp. nov., G. lambkinae sp. nov., G. arcanus sp. nov. and G. vergrandiacus sp. nov. a nd with T1 smooth (in all four of the previously mentioned species, T1 is either rugose or at least clearly punctured in the posterior half). It is noted, however, that all these species are morphologically very similar; limited specimens were available for examination and identifications should be made or corroborated with DNA barcodes. Etymology This species is named for Alison Goodwin and Roger Noakes, who ran a Malaise trap on their property near Bendemeer, NSW, from which a paratype of the species originates. It is a noun in apposition. Material examined Holotype AUSTRALIA ��� ♀; Queensland, Lonesome National Park; -25.495, 148.812; 585 m a.s.l.; 3���26 Nov. 2010; C. Lambkin et al. leg.; near lookout (LNP 4m), closed Eucalyptus woodland on rocky ridge, Malaise trap; Extraction647, BOLD: AUMIC425-18; QM T208399. Paratypes AUSTRALIA - New South Wales ��� 1 ♀; Bendemeer; -30.819, 151.142; 840 m a.s.l.; 9���23 Feb. 2020; A. Goodwin and R. Noakes leg.; Malaise trap, remnant bushland on cattle-grazed property; Extraction923, BOLD: AUGLY018-21; QM T250956. ��� Queensland ��� 1 ♀; West Quinkan, Bush Blitz Site F3; -15.812550, 144.41974; 495 m a.s.l.; 6���15 Mar. 2017; B. Baehr leg.; open woodland; Extraction895, BOLD: AUGLY017-21; QM T250957. Description Female COLOURATION. Gena without a pale spot; labrum mostly dark; scape colour in ventral half uniformly paler than flagellomeres; flagellomeres all black/dark brown; tegula light brown; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark, or dark with very slight orange patches on posterolateral corners; scutellar disk and metanotum dark; propodeum dark; fore coxa dark; mid coxa dark; hind coxa dark; fore femur pale yellow; mid femur orange to light brown; hind femur dark reddish-brown or orange to light brown; fore tibia pale yellow; mid tibia orange to light brown; hind tibia darkening posteriorly; hind basitarsus dark reddish-brown; T1 dark; T2 sclerotised area dark; T2 lateral area same colour as sclerotised area, or only slightly paler or dark extends past indentation, but then pale; T3 dark; T4+ dark. HOLOTYPE BODY MEASUREMENTS. Body length 2.2 mm; fore wing length 2.1 mm; antennal length similar to body length. HEAD. Antennal flagellomere 14 length/width 1.50���1.66; antennal flagellomere 2 length/width 2.62��� 3.50; OOD/POD 1.71���2.00; IOD/POD 1.43���1.67. MESOSOMA. Anteromesoscutum sculpturing with shallow to deep punctures, space between punctures a mixture of smaller than diameter of punctures and of similar size. Often smoother in posterior centre; scutellar disk sculpturing with shallow punctures scattered over most of area; 8���10 pits in scutellar sulcus; propodeum with median carina absent and propodeum strongly rugose, coarse sculpturing over most of propodeum, sometimes sculpturing clearly deeper in a ���v��� shape with smooth anterior centre area. WINGS. Pterostigma length 0.46 mm; pterostigma width 0.15 mm; r 0.17 mm; 2RS 0.12 mm; 2m 0.07 mm; (RS+M)b 0.08 mm. METASOMA. T1 lateral edges parallel for anterior �� to ⅔ of length, then narrowing posteriorly; T1 smooth and shiny, a few scattered punctures; T1 length 0.34 mm; T1 width at posterior edge 0.11 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.17 mm; T2 width at posterior edge 0.12 mm; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks The wingless barcode for this species is shared with G. doreyi sp. nov. and G. arcanus sp. nov. and differs by 1 bp from the barcodes of G. vergrandiacus sp. nov., G. doreyi sp. nov. (second haplotype) and G. lessardi sp. nov. This group of species is very closely related and would benefit from future, more detailed taxonomic work to ensure that they are not simply divergent populations of the same species. For this species hypothesis, we have made the decision to split them into different species based on the COI barcode sequences being>3% divergent; however, we note that this is a hypothesis and is open to change with future work. Glyptapanteles goodwinnoakes sp. nov. constitutes BIN BOLD:ADL3801 and is 3.69% (p-dist.) divergent from the closet BIN in the database (BOLD:AEI4383; an undescribed lineage, from Australia). Using the BOLD Batch ID engine, the COI sequence of the holotype is 6.1% different from the most similar COI sequence from an Australian specimen (AUGLY141-21; an undescribed lineage, with a single specimen). Distribution This species has a very disjunct distribution, found in northern QLD and in central NSW. This likely reflects poor sampling rather than the true species distribution., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 67-68, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles drioplanetus Fagan-Jeffries & Austin, 2021 Figs 15A, 31 Diagnosis Glyptapanteles drioplanetus can be separated from the other described species of Glyptapanteles in Australia by the combination of the following characters: gena without a pale spot, T1 and T2 dark, anteromesoscutum very smooth, with only shallow and regular punctures, no orange markings on the postero-lateral anteromesoscutum, propodeum very smooth with only shallow punctures and the median carina entirely absent, T1 narrowing slightly for most of length, distal ventral half of antennal scape darker than flagellomeres, hind femur pale anteriorly, darkening posteriorly, tegula pale, labrum pale. Material examined Holotype AUSTRALIA • ♀; South Australia, Macclesfield Primary School; -35.17084, 138.84025; 14 Oct.–6 Nov. 2019; E. Fagan-Jeffries and Macclesfield PS yr. 5–7 class leg.; Malaise Trap EFJ2020MT9; Extraction1033, BOLD: AUMIC549-20; SAMA 32-45153. Paratypes AUSTRALIA – South Australia • 1 ♀; Douglas Scrub; -35.1844, 138.6; 27–28 Sep. 2010; G.S. Taylor and S. Mantel leg.; Malaise Trap 2010 001; Extraction79, BOLD: AUMIC507-18; SAMA 32-45154 • 1 ♀; Gleneagle State Forest; -32.2711, 116.163; 29 Nov. 2005; M.S. Harvey leg.; Malaise trap; Extraction117, BOLD: AUMIC019-18; WAM WAME10965. Remarks This species was recently described and we therefore only provide a diagnosis here. Glyptapanteles drioplanetus constitutes BIN: BOLD:ADL3660 and is 2.56% (p-dist.) from the nearest neighbour (BOLD:AEI2300; an undescribed lineage from Australia, with two specimens).

Glyptapanteles operculinae (Fullaway, 1941) Fig. 16D Diagnosis This species has a distinctive scutellum, with the lateral band extremely broad so that the lateral scutellum is compressed into a narrow crenulated sulcus. This, along with the T1 being extremely sharply narrowing posteriorly from a broad anterior width, means this species is very unlikely to be conspecific with any of the newly described Australian species. Material examined No specimens examined; illustrations and redescription in Austin & Dangerfield (1992) used as reference (Fig. 16D). Distribution American Samoa, Western Samoa., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on page 94, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052, {"references":["Austin A. D. & Dangerfield P. C. 1992. Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics 6: 1 - 76. https: // doi. org / 10.1071 / IT 9920001"]}

Glyptapanteles phytometrae (Wilkinson, 1928) Fig. 47C, E Diagnosis Glyptapanteles phytometrae can be separated from many of the Australian species as follows: from the G. albigena species group by having the gena without a pale spot, from the G. mouldsi and G. eburneus species groups by having T1���2 dark and from the niveus species group by having a relatively smooth anteromesoscutum. The propodeum of G. phytometrae is mostly smooth with rugose sculpturing in the centre (Fig. 47C), which is distinct (at least on the available specimens) from any of the newly described species from Australia. Whilst propodeal sculpturing can vary slightly amongst individuals, we do not consider it likely that G. phytometrae is conspecific with any of the Australian species. Material examined Holotype SAMOA ��� ♀; ��� Samoan Is. P.A. Buxton and G.H. Hopkins ���; ���Pres. by Imp. Bur. Ent. Brit. Mus. 1928- 318���; NHMUK: 3.c.1016 (only images examined). Remarks Known hosts in Samoa include Chrysodeixis eriosoma (Doubleday, 1843) and an unknown Plusiinae (Noctuidae). Distribution (in the Australasian region) Samoa, Fiji, Sumatra (Austin & Dangerfield 1992)., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on page 94, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052, {"references":["Wilkinson D. S. 1928. A revision of the Indo-Australian species of the genus Apanteles (Hym. Bracon.). Part II. Bulletin of Entomological Research 19: 109 - 146. https: // doi. org / 10.1017 / S 0007485300020393","Austin A. D. & Dangerfield P. C. 1992. Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics 6: 1 - 76. https: // doi. org / 10.1071 / IT 9920001"]}

Glyptapanteles fullawayi Austin & Dangerfield, 1992 Fig. 16C Diagnosis The combination of a smooth propodeum, T1���2 dark, T1 only narrowing slightly posteriorly and gena without a pale spot, allow G. fullawayi to be separated from the G. albigena, G. mouldsi, G. eburneus, G. niveus and G. arcanus species groups. Glyptapanteles fullawayi is morphologically similar (at least using the available illustrations) to the G. austini species group and to Glyptapanteles drioplanetus. Glyptapanteles fullawayi appears to have only 6���7 large pits in the scutellar sulcus (differing from the G. austini species group and from G. drioplanetus, which all have at least 8); however, this character is prone to variability. Despite this, we feel that it is not very likely that this species is conspecific with any of the newly described species due to the distribution being restricted to Western Samoa. Material examined No specimens examined, illustrations and diagnosis in Austin & Dangerfield (1992) used as reference (Fig. 16). Distribution Western Samoa., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on pages 66-67, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052, {"references":["Austin A. D. & Dangerfield P. C. 1992. Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics 6: 1 - 76. https: // doi. org / 10.1071 / IT 9920001"]}

Glyptapanteles taylori (Wilkinson, 1928) Fig. 47A���B, D Diagnosis As Austin & Dangerfield (1992) state that this species is unlikely to actually occur in the Australasian region and as we were unable to find morphological characters that definitively separate this species from all others in Australia, we do not diagnose it. There is a very low risk of this species being conspecific with any of the newly described species from Australia due to the likelihood that it does not actually occur in the region. Material examined Holotype JAVA ��� ♀; ��� Java 1926 T.N.C. Taylor ���; ���Pres. by Imp. Bur. Ent. Brit. Mus. 1928-318���; NHMUK: 3.c.1018 (only images examined). Distribution (in the Australasian region) Fiji (likely erroneous record), introduced., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on page 106, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052, {"references":["Wilkinson D. S. 1928. A revision of the Indo-Australian species of the genus Apanteles (Hym. Bracon.). Part II. Bulletin of Entomological Research 19: 109 - 146. https: // doi. org / 10.1017 / S 0007485300020393","Austin A. D. & Dangerfield P. C. 1992. Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics 6: 1 - 76. https: // doi. org / 10.1071 / IT 9920001"]}

Glyptapanteles austini Fagan-Jeffries & Bird sp. nov. urn:lsid:zoobank.org:act: 7DAEC136-31EE-4EC2-B197-66F9B686ACEE Figs 15B, 22 Diagnosis Despite the large molecular divergence in both the COI and wingless genes (see Remarks section), the only morphological character found to differentiate G. austini sp. nov. from G. guzikae sp. nov. is the femur being completely dark (G. guzikae sp. nov. with the femur pale with a dark area posteriorly). Glyptapanteles austini sp. nov. can be separated from G. kingae sp. nov. as follows: G. kingae sp. nov. has the propodeum with stronger sculpturing in the centre, whilst G. austini sp. nov. has the propodeum smoother in the centre. However, we note that there are limited specimens available and the variability of propodeal sculpturing could broaden as more populations of these species are identified and we recommend confirming identifications with DNA barcodes. Etymology Named for Professor Andrew Austin, who has championed the documentation of Microgastrinae in Australia and has made a significant impact on the field of systematics of parasitic Hymenoptera, along with providing over a decade of mentorship and training to EPF-J. Material examined Holotype AUSTRALIA • ♀; South Australia, Mt Barker Summit; -35.0661, 138.923; 20 Mar.–3 Apr. 2016; A. Austin and E. Fagan-Jeffries leg.; Malaise trap; Extraction343, BOLD: AUMIC198-18; SAMA 32- 45047. Paratypes AUSTRALIA – South Australia • 1 ♀; same collection data as for holotype; 3–22 Apr. 2016; Extraction340, BOLD: AUMIC196-18; SAMA 32-45048 • 1 ♀ (ethanol); same collection data as for preceding; Extraction210, BOLD: AUMIC095-18; SAMA 32-45049 • 1 ♀ (ethanol); Belair National Park Gate 11; -35.009, 138.654; 11–24 Nov. 2007; J.T. Jennings leg.; Malaise trap; Extraction355, BOLD: AUMIC208-18; SAMA 32-45050 • 1 ♀; same collection data as for preceding; 25 Nov.–1 Dec. 2007; Extraction367, BOLD: AUMIC219-18; SAMA 32-46151 • 1 ♀; Millbrook Reservoir Gate 28, 25 km NE Adelaide; -34.8078, 138.827; 12 Sep. 2007; L. Farrington leg.; Malaise trap; Extraction469, BOLD: AUMIC291-18; SAMA 32-46152. Description Female COLOURATION. Gena without a pale spot; labrum pale or reddish-brown; scape colour in ventral half the same colour or darker than flagellomeres; flagellomeres all black/dark brown; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark; scutellar disk and metanotum dark; propodeum dark; fore coxa dark; mid coxa dark; hind coxa dark; fore femur orange to light brown; mid femur dark proximally and lightening distally; hind femur dark; fore tibia orange to light brown; mid tibia orange to light brown; hind tibia darkening posteriorly; hind basitarsus dark reddish-brown; T1 dark; T2 sclerotised area dark or dark reddish-brown; T2 lateral area much paler; T3 mostly pale with darker patch in centre or mostly dark with paler lateral areas; T4+ dark. BODY MEASUREMENTS. Body length 2.1 mm; fore wing length 2.0 mm; antennal length slightly shorter than body length. HEAD. Face with fine punctures associated with setae; antennal flagellomere 14 length/width 1.83–2.50; antennal flagellomere 2 length/width 2.57–3.85; OOD/POD 1.83–2.00; IOD/POD 1.43–2.17. MESOSOMA. Anteromesoscutum sculpturing with shallow punctures, space between punctures generally smaller than diameter of punctures, slightly denser and deeper punctures anteriorly, smoother in posterior centre; scutellar disk sculpturing with only very shallow punctures; 7–9 pits in scutellar sulcus; propodeum with median carina absent, scattered punctures associated with setae in posterior half, rest of propodeum smooth and shiny. WINGS. Pterostigma length 0.52 mm; pterostigma width 0.18 mm; r 0.12 mm; 2RS 0.11 mm; 2m 0.06 mm; (RS+M)b 0.09 mm. METASOMA. T1 wedge-shaped, narrowing posteriorly for entirety of length, or lateral edges parallel for anterior 1/4 of length, then gently narrowing posteriorly, lateral edges either straight or with slight curve; T1 mostly smooth, some punctures in posterior half; T1 length 0.3 mm; T1 width at posterior edge 0.09 mm; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.11 mm; T2 width at posterior edge 0.19 mm; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks Glyptapanteles austini sp. nov. constitutes BIN BOLD:ADL2587 and is 7.69% (p-dist.) divergent from the closet BIN in the database (BOLD:ADL2952; Glyptapanteles guzikae sp. nov.). Using the BOLD Batch ID engine, the COI barcode of the holotype is 7.9% different from the most similar COI sequence from an Australian specimen (AUMIC524-18; Glyptapanteles guzikae sp. nov.). All five paratype specimens were sequenced for the wingless gene, which is identical amongst all specimens (although there is a single ambiguity in one sequence). The wingless sequences differ by a minimum of 16 bp from all other species with available sequence data. Distribution This species is currently known from SA, from Adelaide and close surrounds.

Glyptapanteles mnesampela Austin, 2000 Figs 6A, 44 Diagnosis Glyptapanteles mnesampela is in the G. albigena species group and can be separated from the other members of the species group by having a pale T2, whilst all other described species with a pale spot on the gena have T2 dark in colouration. Material examined Holotype AUSTRALIA ��� ♀; Australian Capital Territory, Lyneham Ridge; 30 Feb. 1993; ex Mnesampela privata; 22 Jun. 1993; ANIC 32-141445. (Only images examined). Remarks Reared from Mnesampela privata Guen��e, 1858 (Geometridae). Distribution Known from Australia, currently only from the ACT., Published as part of Fagan-Jeffries, Erinn P., McCLELLAND, Alana R., Bird, Andrew J., Giannotta, Madalene M., Bradford, Tessa M. & Austin, Andrew D., 2022, Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species, pp. 1-116 in European Journal of Taxonomy 792 (1) on page 89, DOI: 10.5852/ejt.2022.792.1647, http://zenodo.org/record/6037052

Glyptapanteles Ashmead, 1904 Glyptapanteles Ashmead 1904b: 147. For notes on the Australasian fauna see Austin & Dangerfield (1992) and for a checklist of the world fauna see Fernández-Triana et al. (2020). Type species, by monotypy, Apanteles ashmeadi Wilkinson, 1928: 84, a replacement name for Glyptapanteles manilae Ashmead, 1904a. Comments. The description of a new species of Glyptapanteles will raise the number of species described from Australia from two to three, and this precedes a comprehensive revision of the genus, currently underway, which is expected to include at least 30 species., Published as part of Fagan-Jeffries, Erinn P., Austin, Andrew D. & Investigators, Citizen Science Participants Of Insect, 2021, Four new species of parasitoid wasp (Hymenoptera: Braconidae) described through a citizen science partnership with schools in regional South Australia, pp. 79-101 in Zootaxa 4949 (1) on page 89, DOI: 10.11646/zootaxa.4949.1.4, http://zenodo.org/record/4635871, {"references":["Ashmead, W. H. (1904 b) Descriptions of new genera and species of Hymenoptera from the Philippine Islands. Proceedings of the United States National Museum, 28, 127 - 158. https: // doi. org / 10.5479 / si. 00963801.28 - 1387.127","Austin, A. D. & Dangerfield, P. C. (1992) Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics, 6, 1 - 76. https: // doi. org / 10.1071 / IT 9920001","Fernandez-Triana, J., Shaw, M. R., Boudreault, C., Beaudin, M. & Broad, G. R. (2020) Annotated and illustrated world checklist of Microgastrinae parasitoid wasps (Hymenoptera, Braconidae). ZooKeys, 920, 1 - 1089. https: // doi. org / 10.3897 / zookeys. 920.39128","Wilkinson, D. S. (1928) A revision of the Indo-Australian species of the genus Apanteles (Hym. Bracon.). Part I. Bulletin of Entomological Research, 19, 79 - 105. https: // doi. org / 10.1017 / S 0007485300028856","Ashmead, W. H. (1904 a) A list of the Hymenoptera of the Philippine Islands, with descriptions of new species. Journal of the New York Entomological Society, 12, 1 - 22."]}

Involving the community in taxonomic research has the potential to increase the awareness, appreciation and value of taxonomy in the public sphere. We report here on a trial citizen science project, Insect Investigators, which partners taxonomists with school students to monitor Malaise traps and prioritise the description of new species collected. In this initial trial, four schools in regional South Australia participated in the program and all collected new species of the braconid subfamily Microgastrinae (Hymenoptera: Braconidae). These four species are here described as new, with the names being chosen in collaboration with the participating school students: Choeras ramcomarmorata Fagan-Jeffries & Austin sp. nov., Glyptapanteles drioplanetus Fagan-Jeffries & Austin sp. nov., Dolichogenidea franklinharbourensis Fagan-Jeffries & Austin sp. nov. and Miropotes waikerieyeties Fagan-Jeffries & Austin sp. nov. All four species are diagnosed against the known members of the genera from Australia, New Zealand, Fiji, Samoa and Papua New Guinea, and images and COI DNA barcodes are provided of the holotypes. Students had positive feedback about their experiences of the program, and there is significant potential for it to be expanded and used as a means to connect communities with taxonomic science.

Glyptapanteles drioplanetus Fagan-Jeffries & Austin sp. nov. (Fig. 5) urn:lsid:zoobank.org:act: A33DD298-01DD-47EC-AFFA-AF369D00B898 Material examined. Holotype: South Australia: ♀ Macclesfield Primary School, Bush Block, -35.17084 138.84025, 14.x.2019 – 6.xi.2019, E. Fagan-Jeffries & Macclesfield PS yr 5–7 class, M/T, EFJ2020MT9, Extraction 1033 (SAMA: 32-45153, BOLD: AUMIC549-20). Paratypes: South Australia: ♀ Douglas Scrub, -35.18438 138.59952, 27–28.ix.2010, GS Taylor, S. Mantel M /T, 2010 001, Extraction 79 (SAMA:32-45154, BOLD: AUMIC507-18). Western Australia: ♀ Gleneagle State Forest, 29/xi/2005, M.S. Harvey, M/T, Extraction 117 (WAM: WAME10965, BOLD: AUMIC019-18). Diagnosis. This species can be separated from the currently described species of Glyptapanteles from Australasia in the following ways: • From G. afiamaluanus (Fullaway, 1941), from Samoa, by the absence of a medial carina on the propodeum. • G. artonae (Rohwer, 1926) probably does not occur in the Australasian region (Austin & Dangerfield 1992), but G. drioplanetus can be separated from G. artonae by having T1 considerably more narrow. • G. aucklandensis (Cameron, 1909) was described from a single male from Auckland, NZ, and the type is missing the abdomen. The type is therefore not particularly helpful in providing diagnostic characters, however we feel comfortable determining that it is a different species to G. drioplanetus as there is generally very little overlap in the microgastrine fauna between Australian and New Zealand. • From G. deliasa (Austin & Dangerfield, 1992), from Australia, by the absence of a faint medial carina or striation on the propodeum. • From G. demeter (Wilkinson, 1934), from New Zealand, by having the mesosoma not dorsally-ventrally flattened. • From G. fullawayi Austin & Dangerfield, 1992, from Samoa, by having approximately 9–10 small pits in the scutoscutellar sulcus. • From G. mnesampela Austin, 2000, from Australia, by having T2 dark and the absence of a white gena spot. • G. phytometrae (Wilkinson, 1928). Whilst we were unable to examine the type, as this species is only recorded from Samoa, Sumatra and Fiji, we feel it is extremely unlikely to be the same species. • From G. operculinae (Fullaway, 1941), from Samoa, by having T1 narrowing posteriorly less significantly, not strongly wedge-shaped. • G. taylori (Wilkinson, 1928) probably does not occur in the Australasian region (Austin & Dangerfield 1992), but G. drioplanetus can be separated by having T2 triangular, with straight rather than curved lateral sides. Description. FEMALE. Colour. Head, antenna and mesosoma all dark; all tergites and most of metasoma including hypopygium and ovipositor sheaths dark, non sclerotised areas of T1–2 and anterior sternites pale; (fore-, mid-, hind coxa) pale, pale, dark; (fore-, mid-, hind- trochanter) pale, pale, pale with darker area distally femora (fore-, mid-, hind femur) pale, pale, pale transitioning to dark distally; tibiae (fore-, mid-, hind tibia) pale, pale darkening slightly distally, pale darkening significantly distally; tarsi (fore-, mid-, hind tarsi) light brown, light brown, dark; tegula and humeral complex pale; pterostigma uniformly dark; fore wing veins mostly dark. Body length. Head to apex of metasoma: 2.3 mm (2.4–2.8 mm). Head. Antenna approximately equal to body length; OOL/POD 1.4 (2.0); POL/ POD 2.2 (2.3); antennal flagellomere 2 length/width 2.8 (3.5–3.7); antennal flagellomere 14 length/width 1.6 (2.4). Mesosoma. Anteromesoscutum relatively smooth and shiny, very shallowly, and regularly punctate; number of pits in scutoscutellar sulcus approximately 11, but very irregular in size and definition. Scutellar disc very smooth, with only very shallow pits. Propodeum extremely smooth, no discernible sculpturing or carinae other than very shallow pits associated with scattered setae. Wings. Fore wing length 2.4 mm (2.3–2.9 mm); length of veins r/2RS 1.4 (1.1–1.4); length of veins 2RS/2M 1.3 (1.0–1.5); length of veins 2M/(RS+M)b 0.9 (1.1–1.5); pterostigma length/width 2.4 (2.3–2.5). Legs. Hind tibia inner spur length/hind basitarsus length 0.6 (0.4–0.5). Metasoma. T1 length / T1 width at posterior margin 3.0 (2.4–2.9); narrowing slightly posteriorly (in Douglass Scrub paratype nearly parallel sided) irregularly punctate in posterior half; T2 width at posterior margin / T2 length 1.7 (1.6), subtriangular, smooth in centre, border with T3 shallowly crenulate; T3 sculpture smooth and shiny with scattered setae concentrated in posterior half; ovipositor sheaths length/hind tibial length 0.16 (0.3–0.18); ovipositor gently curved. MALE. Unknown. Etymology. This species is named by the 2020 Year 5/6/7 students of Macclesfield Primary School, who agreed upon a name that means ‘bush wanderer’; drio- from the Greek ‘drios’ for copse or thicket, and planetus from the Greek verb ‘planao’: to wander. The species epithet is an adjective. Distribution. This species is currently only known from two sites in South Australia, and from a single locality in south-western WA. Host. Unknown. Molecular information. This species constitutes the BIN BOLD:ADL3660, and is 2.66% divergent from the nearest neighbour on the BOLD. Remarks. This species, when analysed alongside all available COI sequences of Glyptapanteles, Protapanteles, Sathon (Mason 1981), Lathrapanteles and Cotesia on BOLD, is closely related to species of Protapanteles, including P. alaskensis Ashmead, 1902 (Fig. 6). The morphological distinction between Glyptapanteles and Protapanteles appears to be difficult to define. According to Fernández-Triana et al. (2020), “In Glyptapanteles, T1 is either parallel-sided anteriorly and then strongly narrowing posteriorly, or its sides are gradually to strongly converging posteriorly when compared to Protapanteles which has T1 parallel-sided throughout, except for a strongly rounded apex, and propodeum sculpture that is usually, but not always, more rugose and carinated than in Glyptapanteles.” However, several species of Glyptapanteles, including the paratypes of G. drioplanetus, have T1 of similar shape to that of Protapanteles, and many of the undescribed Glyptapanteles in Australia have a rugose propodeum. As Protapanteles is currently not documented from Australia, and is mostly confined to the Holarctic region (Fernández-Triana et al. 2020), we place G. drioplanetus in Glyptapanteles, but note that it is likely closely aligned with several species of Protapanteles and may need to be reclassified when a better understanding of the generic boundaries for the Microgastrinae is developed., Published as part of Fagan-Jeffries, Erinn P., Austin, Andrew D. & Investigators, Citizen Science Participants Of Insect, 2021, Four new species of parasitoid wasp (Hymenoptera: Braconidae) described through a citizen science partnership with schools in regional South Australia, pp. 79-101 in Zootaxa 4949 (1) on pages 89-93, DOI: 10.11646/zootaxa.4949.1.4, http://zenodo.org/record/4635871, {"references":["Fullaway, D. T. (1941) A check list of the parasitic Hymenoptera of the Samoan Islands with descriptions of new species appeneded. Proceedings of the Hawaiian Entomological Society, 11, 41 - 49.","Rohwer, S. A. (1926) Description of a new braconid parasite of Artona catoxantha (Hymenoptera). Proceedings of the Entomological Society of Washington, 28, 188 - 9.","Austin, A. D. & Dangerfield, P. C. (1992) Synopsis of Australasian Microgastrinae (Hymenoptera: Braconidae), with a key to genera and description of new taxa. Invertebrate Systematics, 6, 1 - 76. https: // doi. org / 10.1071 / IT 9920001","Cameron, P. (1909) Descriptions of four species of Hymenoptera from Auckland Island. Subantarctic Islands of New Zealand, 1, 75 - 7.","Wilkinson, D. S. (1928) A revision of the Indo-Australian species of the genus Apanteles (Hym. Bracon.). Part I. Bulletin of Entomological Research, 19, 79 - 105. https: // doi. org / 10.1017 / S 0007485300028856","Mason, W. R. M. (1981) The polyphyletic nature of Apanteles Foerster (Hymenopera: Braconidae): A phylogeny and reclassification of Microgastrinae. Memoirs of the Entomological Society of Canada, 113, 1 - 147. https: // doi. org / 10.4039 / entm 113115 fv","Ashmead, W. H. (1902) Papers from the Harriman Alaska Expedition XXVIII. Hymenoptera. Proceedings of the Washington Academy of Science, 4, 117 - 268. https: // doi. org / 10.5962 / bhl. part. 18572","Fernandez-Triana, J., Shaw, M. R., Boudreault, C., Beaudin, M. & Broad, G. R. (2020) Annotated and illustrated world checklist of Microgastrinae parasitoid wasps (Hymenoptera, Braconidae). ZooKeys, 920, 1 - 1089. https: // doi. org / 10.3897 / zookeys. 920.39128"]}

Occurrence of Spodoptera frugiperda (J. E. Smith) (Insecta: Lepidoptera: Noctuidae), commonly known as fall armyworm, in southern India is reported along with associated natural enemies. Severe damage was noticed in Chikkaballapur, Hassan, Shivamogga, Davanagere and Chitradurga during July-August 2018. The incidence ranged from 9.0 to 62.5 percent at various locations, maximum incidence was recorded in Hassan district followed by Chikkaballapur, Davanagere, Shivamogga and Chitradurga. Morphology and molecular based taxonomic tools were used for the identification of this pest. The GenBank accession number MH704433 of Chikkaballapur population was released on 1 st August, 2018 and Barcode obtained from BOLD System-ID: AGIMP054-18. The survey also revealed natural parasitism by egg parasitoids viz., Telenomus sp. (Hymenoptera: Platygastridae) and Trichogramma sp. (Hymenoptera: Trichogrammatidae), gregarious larval parasitoid, Glyptapanteles creatonoti (Viereck) (Hymenoptera: Braconidae) solitary larval parasitoid, Campoletis chlorideae Uchida (Hymenoptera: Ichneumonidae), and a solitary indeterminate larval-pupal (Hymenoptera: Ichneumonidae: Ichneumoninae) parasitoid. Spodoptera frugiperda is the first host record for G. creatonoti across the globe. Glyptapanteles creatonoti , being a well established parasitoid of various noctuids in India and Malaysia, was capable of parasitizing S. frugiperda . Besides these, other commonly found bioagents viz., Forficula sp. (Dermaptera: Forficulidae) and entomopathogenic fungus Nomuraea rileyi (Farl.) Samson was also collected in large numbers. We report the natural enemy complex of S. frugiperda for the first time from India. The electro physiological response of Indian population of S. frugiperda male adults to pheromone was established. The studies to manage this pest by any/all means are in progress.

Ten new species within four genera of Microgastrinae parasitoid wasps (Hymenoptera: Braconidae) are described from Canada and United States: Diolcogaster ichiroi, Diolcogaster miamensis, Glyptapanteles pseudotsugae, Microgaster archboldensis, Microgaster syntopic, Microplitis altissimus, Microplitis jorgeluisi, Microplitis juanmanueli, Microplitis julioalbertoi, and Microplitis mariamargaritae. The new taxa are significant because they represent the first North American records of a tropical group (species of the basimacula group in Diolcogaster), exemplify interesting ecological cases (niche-based host selection in Glyptapanteles, syntopic species in Microgaster), and showcase unique morphological features and/or altitudinal records (Microplitis). Most of the new species were collected in protected areas or areas with strong research programs (Archbold Biological Station and hammock forests near Miami, Florida; Great Sand Dunes National Park and Preserve, and Mount Evans Wilderness Area, Colorado; Sapelo Island, Georgia; Tonto National Forest, Arizona), and thus are also of value and interest for conservation and research efforts.

Glyptapanteles taniaariasae Arias-Penna, sp. nov.Fig. 213 Female. Body length 2.92 mm, antenna length 3.88 mm, fore wing length 3.63 mm. Type material. Holotype: ECUADOR • 1♀; EC-34481, YY-A234; Napo, Yanayacu Biological Station, Yanayacu Road; cloud forest; 2,100 m; - 0.566667, -77.866667; 20.vii.2008; Earthwatch volunteers leg.; caterpillar collected in second instar; white solitary cocoon formed on 04.viii.2008; adult parasitoid emerged on 23.xii.2008; (PUCE). Diagnosis. Face with dense fine punctations (Fig. 213B), distal antennal flagellomere subequal in length with penultimate, scutellum in profile convex and slightly higher than mesoscutum (Fig. 213A), petiole on T1 distally with lateral margins convex, petiole parallel-sided but barely narrowing at apex (Fig. 213H), mesoscutum punctation proximally distinct, but distally absent/dispersed (Fig. 213F), dorsal furrow of pronotum with a well-defined smooth band, precoxal groove deep, smooth and shiny (Fig. 213A, J), dorsal carina delimiting a dorsal furrow on propleuron absent. (Fig. 213H), anteroventral contour of mesopleuron straight/angulate or nearly so (Fig. 213A, J), edges of median area on T2 polished and followed by a deep groove (Fig. 213H), and fore wing with r vein curved, outer side of junction of r and 2RS veins forming a stub (Fig. 213L). Coloration (Fig. 213 A–M). General body coloration satin black except apex of labrum, mandibles and pedicel brown-red/reddish; scape and all antennal flagellomeres (on both sides) dark brown; glossa, maxillary and labial palps, and tegulae light yellow-brown. Eyes gray/black and ocelli brownish/reddish (in preserved specimen). Fore and middle legs dark yellow/ light yellow-brown except tibiae and tarsomeres of middle legs with brown tints and claws brown; hind legs dark yellow/light yellow-brown except coxae dorsally with a brown spot, femora distally with a tiny brown area, tibiae distal half brown (coloration intensity increasing from proximal to distal) and proximally with a narrow brown band, and tarsomeres brown, although basitarsus proximally with a yellow band. Petiole on T1 black and sublateral areas yellow-brown; T2 with median and adjacent areas black, adjacent area with contours well-defined, and lateral ends yellow-brown; T3 mostly black except proximal corners of lateral ends; T4 and beyond completely brown; distally each tergum with a narrow yellow translucent band. In lateral view, T1-3 yellow-brown; T3 and beyond yellow-brown, but dorsally brown, extent of brown area remaining relatively constant from proximal to distal. S1-3 yellow-brown, but medially brown; S4 and beyond completely brown. Description. Head (Fig. 213 A–D). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.24:0.09, 0.30:0.09, 0.25:0.09), distal antennal flagellomere subequal in length with penultimate (0.15:0.07, 0.15:0.07), antenna longer than body (3.88, 2.92); antennal scrobes-frons sloped and forming a shelf. Face flat or nearly so, dense fine punctations, interspaces smooth and longitudinal median carina present. Frons smooth. Temple wide, punctate and interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.17). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma (Fig. 213A, F, G, J). Mesosoma dorsoventrally convex. Distal 1/3 of mesoscutum with lateral margin slightly dented, punctation proximally distinct, but distally absent/dispersed, interspaces with microsculpture. Scutellum triangular, apex sloped and fused with BS, but not in the same plane, scutellar punctation scattered throughout, in profile scutellum convex and slightly higher than mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM; ATS demilune with short stubs delineating the area; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation depressed centrally, smooth and shiny. Metanotum with BM wider than PFM (clearly differentiated); MPM circular without median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM; PFM thick, smooth and with lateral ends rounded; ATM proximally with a well-defined row of foveae and distally smooth. Propodeum without median longitudinal carina, proximal half curved with medium-sized sculpture and distal half relatively polished and with a shallow dent at each side of nucha; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by long radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum and dorsal furrow smooth, but ventral furrow with short parallel carinae. Propleuron with a mix of rugae and fine punctation, dorsally without a carina. Metasternum convex. Contour of mesopleuron straight/angulate or nearly so; precoxal groove deep, smooth and shiny; epicnemial ridge widen. Legs (Fig. 213A). Ventral margin of fore telotarsus excavated with conspicuous curved seta over this excavation, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.17, 0.10). Hind coxa with dorsal half sparsely punctate, ventral half densely punctate, and dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.46, 0.31), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.18, 0.16). Wings (Fig. 213L, M). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction forming a distinct stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell completely covered by microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral;vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma (Fig. 213A, H, I, K). Metasoma laterally compressed. Petiole on T1 completely smooth and polished, with faint, satin-like sheen, virtually parallel-sided over most of length, but barely narrowing at apex, apex truncate (length 0.37, maximum width 0.25, minimum with 0.20), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.17, length T2 0.17), lateral grooves deep, median area broader than long (length 0.17, maximum width 0.25, minimum width 0.13); T2 with a distinctive row of pubescence only at the distal margin. T3 longer than T2 (0.26, 0.17) and with a distinctive row of pubescence only at the distal margin. Pubescence on hypopygium dense. Cocoons (Fig. 213E). White or beige oval cocoon with ordered silk fibers, but covered by a net. Comments. The limit between the mesopleuron and the metasternum with a flattened area, the petiole on T1 distally slightly wider and lateral margins more curved (convex) than proximally (Fig. 213H), the body is stout, short and covered with dense pubescence, and the hind coxae is stout (Fig. 213A). Male. Unknown. Etymology. Tania Milena Arias-Penna is a Colombian entomologist. Her research has been focused on taxonomy and systematics of ants (Ponerinae) and parasitoid wasps (Platygastroidea). She is DCAP's sister. Distribution. Parasitized caterpillar was collected in Ecuador, Napo, Yanayacu Biological Station (Yanayacu Road), during July 2008 at 2,100 m in cloud forest. Biology. The lifestyle of this parasitoid species is solitary. Host. Pantherodes unciaria Guenée (Geometridae: Ennominae) feeding on Boehmeria bullata (Urticaceae). Caterpillar was collected in second instar., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles ninazitaniae Arias-Penna, sp. nov.Figs 175, 176 Female. Body length 3.78 mm, antenna length 4.55 mm, fore wing length 3.33 mm. Type material. Holotype: COSTA RICA •1♀; 00-SRNP-23990, DHJPAR0013364; Área de Conservación Guanacaste, Guanacaste, Sector Santa Rosa, Bosque Humedo; dry forest; Malaise; 290 m; 10.85145, -85.60801; 07.ii.2000; DH Janzen & W Hallwachs leg.; (CNC). Paratypes. • 1 (0♀, 1♂) (0♀, 0♂); 00-SRNP-23972, DHJPAR0013603; same data as for holotype except: 17.i.2000; (CNC). • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-23975, DHJPAR0013363; same data as for holotype; (CNC). Other material. Malaise-trapped material. COSTA RICA: Área de Conservación Guanacaste , Guanacaste, Sector El Hacha, Sendero Bejuquilla: • 1 (0♀, 1♂) (0♀, 0♂); 99-SRNP-19251, DHJPAR0013638; dry-rain intergrade forest; Malaise; 280 m; 11.03004, -85.52699; 05.vii.1999; DH Janzen & W Hallwachs leg. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Río Blanco Abajo : • 1 (0♀, 0♂) (0♀, 1♂); 08-SRNP-3559, DHJPAR0027140; rain forest; Malaise; 500 m; 10.90037, -85.37254; 23.iv.2008; DH Janzen & W Hallwachs leg. Diagnosis. Fore wing with vein 2 cu-a present as spectral vein, sometimes difficult to see, vein 1 cu-a straight, r vein slightly curved or curved, outer side of junction of r and 2RS veins not forming a stub (Figs 175K, 176H), dorsal groove on axillary trough of scutellum with parallel carinae (Figs 175F, 176C), propodeum with a median longitudinal dent (Figs 175F, 176C), mesoscutum proximally distinctly punctate, distally with a polished area (Figs 175E, 176B), temple punctate-lacunose, petiole virtually parallel-sided over most of length, but narrowing over distal 1/3, finely sculptured only laterally (Figs 175G, H, 176D, E), dorsal outer depression on hind coxa present (Fig. 175A, J), inner margin of eyes diverging slightly at antennal sockets (Fig. 175B), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (Figs 175G, H, 176D, E). Coloration (Fig. 175 A–L). General body coloration satin black except scape and pedicel yellow-brown with lateral brown band; last four-five distal antennal flagellomeres completely yellow, remaining flagellomeres dark brown on both sides; labrum, mandible, glossa, and tegulae yellow-brown; both ends of propleuron, some small areas of metasternum, a small ventral band of mesopleuron, epicnemial ridge, distal corners of mesoscutum, lateral ends of metapleuron, and distally lunules yellow-brown/reddish; maxillary and labial palps ivory/pale yellow. Eyes and ocelli silver. Fore and middle legs dark yellow, except coxae and trochanter ivory/pale yellow, and claws brown; hind legs pale yellow except coxae proximally with an elongate black spot, femora distally with a tiny brown dot, tibiae and tarsomeres dark yellow. Petiole on T1 dark brown, contours darkened and sublateral areas ivory/pale yellow; T2 with median area black although medially reddish/brown, wide adjacent area yellow-brown, and lateral ends ivory/pale yellow with some tints yellow-brown; T3 yellow with two small brown spots medially; T4 and beyond completely brown; distally each tergum with a narrow ivory/pale yellow transparent band. In lateral view, T1-2 completely ivory/pale yellow; T3 proximal half ivory/pale yellow, distal half with some yellow-brown tints; T4 and beyond completely brown. S1-3 completely yellow; S4 yellow, but medially brown; penultimate sternum and hypopygium brown. Description. Head (Fig. 175 A–D). Head rhomboid with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.33:0.10, 0.32:0.10, 0.32:0.10), distal antennal flagellomere longer than penultimate (0.25:0.11, 0.19:0.11), antenna longer than body (4.55, 3.78); antennal scrobes-frons shallow. Face flat or nearly so, punctate-lacunose, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.12). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma (Fig. 175A, E, F, I). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct proximally with polished area distally, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS, scutellar punctation distinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS mostly overlapping the MPM; ATS demilune with quite a little, complete and parallel carinae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation with a smooth and shiny sloped transverse strip. Metanotum with BM wider than PFM (clearly differentiated); MPM semicircular without median longitudinal carina; AFM with a small lobe and not as well delineated as PFM; PFM thick, smooth and with a distal flat flange; ATM proximally with a groove with some sculpturing and distally with rugae. Propodeum with a median longitudinal dent, but no trace of median longitudinal carina, proximal half curved with rather coarse sculpture and distal half rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with a mix of rugae and fine punctation, dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron convex; precoxal groove deep with transverse lineate sculpture; epicnemial ridge elongated more fusiform (tapering at both ends). Legs. Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout. Hind coxa with punctation only on ventral surface, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.41, 0.15), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Wings (Fig. 175K, L). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; subbasal cell with a small smooth area; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a straight, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma (Fig. 175A, G, H, J). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, virtually parallel-sided over most of length, but narrowing over distal 1/3 (length 0.45, maximum width 0.23, minimum width 0.12) and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.20, length T2 0.20), edges of median area polished and lateral grooves deep, median area broader than long (length 0.20, maximum width 0.24, minimum width 0.10); T2 with scattered pubescence only distally. T3 longer than T2 (0.28, 0.20) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. Unknown. Comments. Specimen with just one fore and one hind leg although the tarsomeres are missing. Male (Fig. 176 A–I). The coloration of antennal flagellomeres is evenly throughout; the entire propleuron is reddish/yellow-brown with some areas darker than others; the mesopleuron with a more distinctive ventral reddish/yellow-brown band; the gena, the clypeus, the mesosternum, the ATS, the dorsal ATS groove and the lunules with reddish/yellow-brown tints; the median area on T2 is yellow-brown with contours darkened and the T3 is completely yellow. Etymology. Nina Michelle Zitani has worked in the systematics and biology of Meteorus (Braconidae: Meteorinae). She works at the University of Western Ontario, Canada. Distribution. The adult parasitoids were collected in Costa Rica, ACG, Sector El Hacha (Sendero Bejuquilla), Sector San Cristóbal (Río Blanco Abajo) and Sector Santa Rosa (Bosque Humedo), during July 1999, January-February 2000, and April 2008 at 280 m, 290 m, and 500 m in dry, dry-rain intergrade, and rain forests. Biology. Unknown. Host. Unknown., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles pamitchellae Arias-Penna, sp. nov.Figs 178, 179 Female. Body length 2.17 mm, antenna length 2.58 mm, fore wing length 2.17 mm. Type material. Holotype: COSTA RICA •1♀; 00-SRNP-24057, DHJPAR0024693; Área de Conservación Guanacaste, Guanacaste, Sector Santa Rosa, Bosque Humedo; dry forest; Malaise; 290 m; 10.85145, -85.60801; 08.v.2000; DH Janzen & W Hallwachs leg.; (CNC). Paratypes. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24012, DHJPAR0013610; same data as for holotype except: 01.v.2000; (CNC). • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-24013, DHJPAR0013361; same data as for holotype except: 17.v.2000; (CNC). • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-24016, DHJPAR0013362; same data as for holotype except: 15.v.2000; (CNC). • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-24017, DHJPAR0013358; same data as for holotype except: 15.v.2000; (CNC). Other material. Malaise-trapped material. COSTA RICA: Área de Conservación Guanacaste , Guanacaste, Sector El Hacha, Sendero Bejuquilla: • 1 (1♀, 0♂) (0♀, 0♂); 98-SRNP-16034, DHJPAR0012621; dry-rain intergrade forest; Malaise; 280 m; 11.03004, -85.52699; 07.ix.1998; DH Janzen & W Hallwachs leg. • 1 (0♀, 0♂) (1♀, 0♂); 99-SRNP-18932, DHJPAR0012626; same data as for preceding except: 10.v.1999. • 1 (0♀, 1♂) (0♀, 0♂); 99-SRNP-19262, DHJPAR0013649; same data as for preceding except: 08.ii.1999. • 1 (0♀, 0♂) (1♀, 0♂); 99-SRNP-19266, DHJPAR0013653; same data as for preceding except: 01.ii.1999. Área de Conservación Guanacaste , Guanacaste, Sector Santa Rosa, Estación San Gerardo : • 1 (1♀, 0♂) (0♀, 0♂); 08-SRNP-2835, DHJPAR0026279; Malaise; rain forest; 575 m; 10.88009, -85.38887; 15.iv.2008; DH Janzen & W Hallwachs leg. Área de Conservación Guanacaste , Guanacaste, Sector Santa Rosa, Río Blanco Abajo : • 1 (0♀, 1♂) (0♀, 0♂); 08-SRNP-3240, DHJPAR0026821; Malaise; rain forest; 500 m; 10.90037, -85.37254; 30.iii.2008; DH Janzen & W Hallwachs leg. Área de Conservación Guanacaste , Guanacaste, Sector Santa Rosa, Bosque Humedo: • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16107, DHJPAR0013570; dry forest; Malaise; 290 m; 10.85145, -85.60801; 09.iii.1998; DH Janzen & W Hallwachs leg. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16108, DHJPAR0013349; same data as for preceding. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16110, DHJPAR0013571; same data as for preceding except: 23.iii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16111, DHJPAR0013572; same data as for preceding. • 1 (0♀, 1♂) (0♀, 0♂); 98-SRNP-16114, DHJPAR0013573; same data as for preceding • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16116, DHJPAR0013574; same data as for preceding except: 23.ii.1998. • 1 (0♀, 1♂) (0♀, 0♂); 98-SRNP-16118, DHJPAR0013348; same data as for preceding except: 16.ii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16119, DHJPAR0013575; same data as for preceding. • 1 (1♀, 0♂) (0♀, 0♂); 98-SRNP-16121, DHJPAR0013375; same data as for preceding except: 16.iii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16122, DHJPAR0013576; same data as for preceding except: 23.ii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16123, DHJPAR0013577; same data as for preceding except: 23.ii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16126, DHJPAR0013578; same data as for preceding except: 23.ii.1998. • 1 (1♀, 0♂) (0♀, 0♂); 98-SRNP-16128, DHJPAR0013369; same data as for preceding except: 12.i.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16176, DHJPAR0024666; same data as for preceding except: 26.i.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16180, DHJPAR0024670; same data as for preceding except: 09.ii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16182, DHJPAR0024672; same data as for preceding except: 09.ii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16188, DHJPAR0024678; same data as for preceding except: 09.ii.1998. • 1 (0♀, 0♂) (1♀, 0♂); 98-SRNP-16190, DHJPAR0024680; same data as for preceding except: 09.ii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19077, DHJPAR0013580; same data as for preceding except: 03.v.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19084, DHJPAR0013582; same data as for preceding except: 24.v.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19087, DHJPAR0013584; same data as for preceding except: 03.v.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19088, DHJPAR0013585; same data as for preceding except: 03.v.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19092, DHJPAR0013586; same data as for preceding except: 24.v.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19097, DHJPAR0013588; same data as for preceding except: 24.v.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19098, DHJPAR0013589; same data as for preceding except: 10.v.1999. • 1 (0♀, 1♂) (0♀, 0♂); 99-SRNP-19099, DHJPAR0013590; same data as for preceding except: 24.v.1999. • 1 (0♀, 0♂) (1♀, 0♂); 99-SRNP-19101, DHJPAR0013368; same data as for preceding except: 26.iv.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19107, DHJPAR0013592; same data as for preceding except: 03.v.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19272, DHJPAR0024686; same data as for preceding except: 17.v.1999. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23953, DHJPAR0013593; same data as for preceding except: 29.v.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23954, DHJPAR0013594; same data as for preceding except: 10.iv.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23956, DHJPAR0013595; same data as for preceding except: 28.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23958, DHJPAR0013597; same data as for preceding except: 29.v.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23959, DHJPAR0013598; same data as for preceding except: 29.v.2000. • 1 (1♀, 0♂) (0♀, 0♂); 00-SRNP-23960, DHJPAR0013370; same data as for preceding except: 10.iv.2000. • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-23966, DHJPAR0013372; same data as for preceding except: 31.i.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23970, DHJPAR0013601; same data as for preceding except: 21.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23971, DHJPAR0013602; same data as for preceding except: 24.iv.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23978, DHJPAR0013604; same data as for preceding except: 10.iv.2000. • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-23981, DHJPAR0013365; same data as for preceding except: 21.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23986, DHJPAR0013606; same data as for preceding except: 21.ii.2000. • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-23987, DHJPAR0013367; same data as for preceding except: 07.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23992, DHJPAR0013415; same data as for preceding except: 07.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23995, DHJPAR0013413; same data as for preceding except: 28.ii.2000. • 1 (0♀, 1♂) (0♀, 0♂); 00-SRNP-23996, DHJPAR0013417; same data as for preceding except: 24.i.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24010, DHJPAR0013418; same data as for preceding except: 27.iii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24018, DHJPAR0013412; same data as for preceding except: 05.vi.2000. • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-24022, DHJPAR0013360; same data as for preceding except: 06.iii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24023, DHJPAR0013611; same data as for preceding except: 17.iv.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24024, DHJPAR0013612; same data as for preceding except: 17.iv.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24027, DHJPAR0013406; same data as for preceding except: 21.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24028, DHJPAR0013613; same data as for preceding except: 21.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24029, DHJPAR0013409; same data as for preceding except: 06.iii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24030, DHJPAR0013410; same data as for preceding except: 06.iii.2000. • 1 (1♀, 0♂) (0♀, 0♂); 00-SRNP-24031, DHJPAR0013353; same data as for preceding except: 28.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24033, DHJPAR0013614; same data as for preceding except: 21.ii.2000. • 1 (1♀, 0♂) (0♀, 0♂); 00-SRNP-24034, DHJPAR0013366; same data as for preceding except: 21.ii.2000. • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-24035, DHJPAR0013355; same data as for preceding except: 21.ii.2000. • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-24038, DHJPAR0013356; same data as for preceding except: 28.ii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24052, DHJPAR0024688; same data as for preceding except: 13.iii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24053, DHJPAR0024689; same data as for preceding except: 13.iii.2000. • 1 (0♀, 0♂) (1♀, 0♂); 00-SRNP-24061, DHJPAR0024697; same data as for preceding except: 24.i.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24062, DHJPAR0024698; same data as for preceding except: 24.i.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24066, DHJPAR0024702; same data as for preceding except: 20.iii.2000. • 1 (1♀, 0♂) (0♀, 0♂); 00-SRNP-24067, DHJPAR0024703; same data as for preceding except: 02.i.2000. • 1 (0♀, 0♂) (0♀, 1♂); 07-SRNP-15055, DHJPAR0013615; same data as for preceding except: 05.vi.2007; AR Deans & J Rodriguez. • 1 (0♀, 0♂) (0♀, 1♂); 07-SRNP-15056, DHJPAR0013616; same data as for preceding except: 05.vi.2007; AR Deans & J Rodriguez leg. • 1 (0♀, 0♂) (1♀, 0♂); 07-SRNP-15059, DHJPAR0013354; same data as for preceding except: 05.vi.2007; AR Deans & J Rodriguez leg. Área de Conservación Guanacaste , Guanacaste, Sector Santa Rosa, Bosque San Emilio: • 1 (1♀, 0♂) (0♀, 0♂); 98-SRNP-16066, DHJPAR0013391; dry forest; Malaise; 300 m; 10.84389, -85.61384; 26.x.1998; DH Janzen & W Hallwachs leg. • 1 (1♀, 0♂) (0♀, 0♂); 98-SRNP-16074, DHJPAR0013388; same data as for preceding except: 14.ix.1998. • 1 (0♀, 1♂) (0♀, 0♂); 98-SRNP-16078, DHJPAR0013530; same data as for preceding except: 31.viii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16080, DHJPAR0013531; same data as for preceding except: 24.viii.1998. • 1 (0♀, 0♂) (1♀, 0♂); 98-SRNP-16082, DHJPAR0013532; same data as for preceding except: 31.viii.1998. • 1 (0♀, 0♂) (1♀, 0♂); 98-SRNP-16083, DHJPAR0013389; same data as for preceding except: 14.ix.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16091, DHJPAR0013533; same data as for preceding except: 28.ix.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16096, DHJPAR0013534; same data as for preceding except: 07.ix.1998. • 1 (0♀, 0♂) (0♀, 1♂); 98-SRNP-16102, DHJPAR0013535; same data as for preceding except: 31.viii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-18971, DHJPAR0013538; same data as for preceding except: 08.ii.1999. • 1 (0♀, 0♂) (1♀, 0♂); 99-SRNP-18973, DHJPAR0013539; same data as for preceding except: 11.i.1999. • 1 (0♀, 1♂) (0♀, 0♂); 99-SRNP-19001, DHJPAR0013382; same data as for preceding except: 19.x.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19047, DHJPAR0013548; same data as for preceding except: 18.i.1999. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19068, DHJPAR0013553; same data as for preceding except: 22.iii.1999. • 1 (0♀, 0♂) (0♀, 1♂); 07-SRNP-15070, DHJPAR0013662; same data as for preceding except: 01.v.2007. Diagnosis. Ventral margin of fore telotarsus entire without seta, medioanterior pit of metanotum circular without median longitudinal carina (Figs 178F, 179B), propleuron with fine rugae, dorsal carina delimiting a dorsal furrow present (Figs 178A, I, 179A, G), antenna longer than body, anterior furrow of metanotum with a small lobe, without setae (Figs 178F, 179B), distal antennal flagellomere longer than penultimate, surface of metasternum convex, precoxal groove deep with lineate sculpture (Figs 178A, I, 179A, G), fore wing with vein 1 cu-a curved, r vein curved (Figs 178K, 179D), dorsal outer depression on hind coxa present (178A, J, 179A, F), inner margin of eyes diverging slightly at antennal sockets (Fig. 178B), petiole on T1 finely sculptured only distally (Figs 178G, H, 179C), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (Figs 178G, H, 179C). Coloration (Fig. 178 A–K). General body coloration black except scape yellow with lateral brown band; all antennal flagellomeres dark brown on both sides; labrum, mandible, and tegulae dark yellow; maxillary and labial palps ivory/pale yellow; clypeus, both ends of propleuron, ventral furrow of pronotum, adjacent area of mesoscutum, epicnemial ridge, narrow band taking the place of notauli, distal corners of mesoscutum, lunules, lateral ends of metapleuron, and PFM with yellow-brown/reddish tints. Eyes and ocelli silver. Fore and middle legs dark yellow except coxae trochanters and trochantellus ivory/pale yellow, and claws brown; hind legs pale dark yellow except coxae 2/3 proximal black, femora distally with a tiny brown dot, tibiae distally brown, and tarsomeres brown. Petiole on T1 with two coloration: 3/4 proximally dark yellow-brown, 1/4 distal brown, contours yellow-brown/reddish, and sublateral areas ivory/pale yellow; T2 with median area brown, but proximally with a small yellow-brown/reddish spot, narrow adjacent area yellow-brown, and lateral ends ivory/pale yellow; T3 broadly with a oval-shaped area, proximally width of that dark area coinciding with the width of median and adjacent areas on T2, and lateral ends proximal half of ivory/pale yellow and distal half yellow-brown; T4 and beyond completely brown; distally each tergum with a narrow yellowish transparent band. In lateral view, T1-3 ivory/pale yellow; T4 and beyond brown. S1-3 ivory/pale yellow; S4-5 yellow, but medially brown; hypopygium completely brown. Description. Head (Fig. 178 A–D). Head rectangular with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.19:0.06, 0.19:0.06, 0.19:0.06), distal antennal flagellomere longer than penultimate (0.11:0.06, 0.09:0.06), antenna longer than body (2.58, 2.17); antennal scrobes-frons shallow. Face flat or nearly so, punctate-lacunose, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL subequal in length with OOL (0.09, 0.10). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma (Fig. 178A, E, F, I). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct proximally with polished area distally, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS, scutellar punctation distinct throughout, in profile scutellum slightly convex, but on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM; ATS demilune with quite a little, complete and parallel carinae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM wider than PFM (clearly differentiated); MPM circular without median longitudinal carina; AFM with a small lobe and not as well delineated as PFM; PFM thick, smooth and with lateral ends rounded; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum with transverse rugae, proximal half curved with medium-sized sculpture and distal half rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with fine rugae and dorsally with a carina. Metasternum convex. Contour of mesopleuron convex; precoxal groove deep transverse lineate sculpture; epicnemial ridge elongated more fusiform (tapering at both ends). Legs. Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.10, 0.05). Hind coxa medially smooth, dorsally with scattered punctation and ventrally with dense punctation, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.23, 0.18), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus as equal in length as fourth tarsomere (0.11, 0.10). Wings (Fig. 178K). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with a small smooth area; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma (Fig. 178A, G, H, J). Metasoma laterally compressed. Petiole on T1 finely sculptured only distally, petiole evenly narrowing distally (length 0.30, maximum width 0.15, minimum width 0.06) and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.11, length T2 0.11), edges of median area polished and lateral grooves deep, median area broader than long (length 0.11, maximum width 0.22, minimum width 0.05); T2 with scattered pubescence only distally. T3 longer than T2 (0.18, 0.11) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. Unknown. Comments. The inner side of ATS proximally with a large elongate fovea at each side (Fig. 178F). Male (Fig. 179 A–G). The body shape and the coloration are similar to female. Etymology. Pamela A. Mitchell (RIP) was Ian Gauld's wife who helped with many Hymenoptera identifications from Costa Rica. She was a real entomological partner with Ian Gauld, as well as sorting an enormous number of Malaise-trap samples from ACG and elsewhere. Distribution. The adult parasitoids were collected in Costa Rica, ACG, Sector El Hacha (Sendero Bejuquilla) and Sector Santa Rosa (Bosque Humedo, Bosque San Emilio, Estación San Gerardo, and Río Blanco Abajo), during January–March and August–October 1988, January–May and October 1999, January–June 2000, May–June 2007, and Mach–April 2008 at 290 m, 300 m, 500 m, and 575 m in dry, dry-rain intergrade, and rain forests. Biology. Unknown. Host. Unknown., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles jaquioconnorae Arias-Penna, sp. nov.Fig. 115 Female. Body length 3.13 mm, antenna length 3.53 mm, fore wing length 3.33 mm. Type material. Holotype: ECUADOR • 1♀; EC-2997, YY-A025; Napo, Yanayacu Biological Station, Yanayacu Road; cloud forest; 2,100 m; - 0.566667, -77.866667; 29.v.2005; CAPEA leg.; caterpillar instar not reported; adult parasitoid emerged on 07.vi.2005; (PUCE). Diagnosis. Distal 1/4 of mesoscutum with a central dent (Fig. 115F), medioposterior band of scutellum only very partially overlapping the medioanterior pit of metanotum (Fig. 115F, G), median area on T2 slightly longer than broad, lateral grooves delimiting the median area clearly defined and reaching the distal edge of T2, median area distally with lateral margins relatively straight, edges of median area polished and followed by a deep groove (Fig. 115E, H), scutellum in profile flat, fore wing with vein 2-1A tubular throughout, r vein curved, outer side of junction of r and 2RS veins forming a distinct stub (Fig. 115K), dorsal carina delimiting a dorsal furrow on propleuron absent (Fig. 115C, I), anterior furrow of metanotum without setiferous lobes (Fig. 115F, G), axillary trough of scutellum with sculpture (Fig. 115F, G), propodeum without median longitudinal carina (Fig. 115G), and anteroventral contour of mesopleuron convex (Fig. 115A, I). Coloration (Fig. 115 A–L). General body coloration brown-black although propleuron, dorsal and ventral furrows of pronotum, ventrally mesopleuron, distal corners of mesoscutum, lunules, and PFM with light brown/reddish tints; labrum, glossa, maxillary and labial palps, and tegulae yellow; mandibles, scape and pedicel yellow-brown. Eyes silver and ocelli brownish. Fore and middle legs dark yellow, although tarsomeres with a brownish tint; hind legs dark yellow except coxae dark brown with apex light brown, femora apically with a tiny brown dot, tibiae with 1/3 distal brown and proximally with a narrow brown band, and tarsomeres light brown, but basitarsus proximally with a narrow yellow band. Petiole on T1 brown, contours darkened and sublateral areas ivory/pale yellow; T2 with median area brown, contours darkened, median and wide adjacent areas brown, and lateral ends ivory/pale yellow; T3 brown with proximal corners ivory/pale yellow, and distal corners each one with a oval pale ivory/pale yellow spots; T4 and beyond completely brown; distally each tergum with a yellowish transparent band. In lateral view, T1-2 completely ivory/pale yellow; T3 and beyond yellow, but dorsally brown, extent of brown area increasing slightly from proximal to distal. S1-3 ivory/yellow; S4-5 yellow, but medially brown; hypopygium yellow-brown. Description. Head (Fig. 115 A–D). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.25:0.07, 0.26:0.07, 0.25:0.07), distal antennal flagellomere longer than penultimate (0.16:0.05, 0.11:0.05), antenna longer than body (3.53, 3.13); antennal scrobes-frons shallow. Face with fine and punctate-lacunose sculpture, lateral depression only middle, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate and interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.09, 0.13). Malar suture absent or difficult to see. Median area between lateral ocelli slightly depressed. Vertex laterally rounded and dorsally wide. Mesosoma (Fig. 115A, F, G, I). Mesosoma dorsoventrally convex. Mesoscutum 1/4 distal with a central dent, punctation distinct throughout, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS, but not in the same plane, scutellar punctation scattered throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum completely concealed; BS only very partially overlapping the MPM; ATS demilune with complete undulate/reticulate carinae; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation sloped and with same kind of sculpture as mesoscutum. Metanotum with BM upward; MPM oval/circular with a short proximal carina; AFM without setiferous lobes and not as well delineated as PFM; PFM thick and smooth; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum without median longitudinal carina, proximal half weakly curved with fine sculpture and distal half slightly rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum and dorsal furrow smooth, but ventral furrow with short parallel carinae. Propleuron with fine punctations throughout and dorsally without a carina. Metasternum convex. Contour of mesopleuron convex; precoxal groove deep, smooth and shiny; epicnemial ridge convex, teardrop-shaped. Legs. Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.12, 0.09). Hind coxa finely punctate throughout, and dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.24, 0.20), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.16, 0.13). Wings (Fig. 115K, L). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction forming a slight stub; 2M vein straight; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A tubular throughout; tubular vein 1 cu-a curved, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally and subproximally evenly convex, and setae evenly scattered in the margin. Metasoma (Fig. 115A, E, H, K). Metasoma cylindrical. Petiole on T1 finely sculptured only distally, parallel-sided in proximal half and then narrowing (length 0.40, maximum width 0.22, minimum width 0.12), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.22, length T2 0.22), lateral grooves deep, median area longer than broad (length 0.22, maximum width 0.20, minimum width 0.08); T2 with pubescence only distally. T3 longer than T2 (0.27, 0.22) and with scattered pubescence only distally. Pubescence on hypopygium dense. Cocoons. Unknown. Comments. Some of the first proximal antennal flagellomeres seem to have more of three dark bands (multi-rings). A whole specimen was used for DNA extraction. Male. Unknown. Etymology. Jaqueline (Jaqui) Megan O'Connor is from United Kingdom. As a graduate student at UIUC, IL, USA she worked on systematics and host use of Parapanteles and Cotesia from Ecuador and Costa Rica. Currently, she is a biology teacher at a school called Christ's Hospital, Horsham, West Sussex, England, and dedicated to social change. Distribution. Parasitized caterpillar was collected in Ecuador, Napo, Yanayacu Biological Station (Yanayacu Road), during May 2005 at 2,100 m in cloud forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Undetermined species of Nymphalidae (Ithomiinae) feeding on undetermined species of Solanaceae. Caterpillar instar was not reported., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles bobhanneri Arias-Penna, sp. nov.Figs 31, 32 Female. Body length 2.53 mm, antenna length 2.98 mm, fore wing length 2.78 mm. Type material. Holotype: COSTA RICA • 1♀; 04-SRNP-33819, DHJPAR0001511]; Área de Conservación Guanacaste, Guanacaste, Sector Pitilla, Sendero Mismo; rain forest; 680 m; 10.98758, -85.41967; 01.vii.2004; Manuel Rios leg.; caterpillar collected in fourth instar; bud-like cocoons, scattered loose, adhered to the leaf substrate and formed on 11.vii.2004; adult parasitoids emerged on 17.vii.2004; (CNC). Paratypes. • 3 (1♀, 1♂) (1♀, 0♂); 04-SRNP-33819, DHJPAR0001511; same data as for holotype; (CNC). Other material. Reared material. COSTA RICA: Área de Conservación Guanacaste , Guanacaste, Sector Pitilla, Sendero Mismo: • 3 (2♀, 0♂) (1♀, 0♂); 04-SRNP-33811, DHJPAR0001473; rain forest; 680 m; 10.98758, -85.41967; 01.vii.2004; Petrona Rios leg.; caterpillar collected in fourth instar; white ridged bud-like cocoons adhered to the leaf substrate and formed on 09.vii.2004; adult parasitoids emerged on 17.vii.2004. • 5 (2♀, 0♂) (3♀, 0♂); 04-SRNP-33810, DHJPAR0001502; same data as for preceding except: scattered white bud-like cocoons adhered to the leaf substrate and formed on 06.vii.2004; adult parasitoids emerged on 14.vii.2004. • 3 (1♀, 1♂) (1♀, 0♂); 04-SRNP-33813, DHJPAR0001515; same data as for preceding except: caterpillar collected in third instar; white bud-like cocoons formed on 06.vii.2004; adult parasitoids emerged on 14.vii.2004. • 2 (0♀, 1♂) (0♀, 1♂); 04-SRNP-33808, DHJPAR0001518; same data as for preceding except: white bud-like cocoons formed on 06.vii.2004; adult parasitoids emerged on 14.vii.2004. • 2 (1♀, 0♂) (1♀, 0♂); 04-SRNP-33648, DHJPAR0001525; same data as for preceding except: 23.vi.2004; Manuel Rios leg.; caterpillar collected in third instar; white bud-like cocoons formed on 22.vii.2004 and adhered to the leaf substrate; adult parasitoids emerged on 28.vii.2004. Área de Conservación Guanacaste , Guanacaste, Sector Pitilla, Sendero Laguna; • 3 (2♀, 0♂) (1♀, 0♂); 04-SRNP-33351, DHJPAR0001531; rain forest; 680 m; 10.9888, -85.42336; 15.vi.2004; Calixto Moraga leg.; caterpillar collected in third instar; white bud-like cocoons adhered to the leaf substrate and formed on 27.vi.2004; adult parasitoids emerged on 09.vii.2004. Área de Conservación Guanacaste , Guanacaste, Sector Pitilla, Estación Quica : • 2 (1♀, 0♂) (1♀, 0♂); 08-SRNP-71182, DHJPAR0031102; rain forest; 470 m; 10.99697, -85.39666; 07.vii.2008; Oscar Siezar leg.; caterpillar collected in third instar; white bud-like cocoons adhered to the leaf substrate and formed on 29.vii.2008; adult parasitoids emerged on 06.viii.2008. Diagnosis. Precoxal groove smooth and shiny (Figs 31A, G, H, 32A), medioanterior pit of metanotum circular without median longitudinal carina (Figs 31G, 32G), inner margin of eyes straight throughout, scutellar punctation indistinct throughout (Figs 31G, 32G), fore wing with 1 cu-a vein complete, touching the edge of 1-1A vein, outer side of junction of r and 2RS veins not forming a stub (Figs 31L, 32L), propodeum with a median longitudinal carina (Figs 31G, 32G), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (Figs 31H, 32K). Coloration (Fig. 31A). General body coloration dark brown black except labrum, mandibles, glossa, maxillary and labial palps, and tegulae yellow-brown. Eyes silver and ocelli silver/yellowish. Fore and middle legs yellow-brown, fore claws brown; hind legs yellow-brown except coxae brown-black with apex yellow-brown, apex of femora, both ends of tibiae brown and tarsomeres brown although tarsomeres 1-3 with a proximal yellow-brown ring. Petiole on T1 black-brown and sublateral areas yellow-brown; T2 with median area brown with contours darkened, adjacent area narrow and brown, and lateral ends yellow-brown; T3 and beyond yellow-brown/brown; distally each tergum with a narrow whitish band. In lateral view, T1-2 completely yellow; T3 and beyond yellow, but dorsally brown, extent of brown area remains constant in each tergum. All sterna yellow, but hypopygium medially brown and some lateral parts too; ovipositor sheaths brown. Description. Head (Fig. 31 A–D). Head triangular with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.25:0.08, 0.25:0.08, 0.24:0.08), distal antennal flagellomere longer than penultimate (0.12:0.06, 0.10:0.05), antenna longer than body (2.98, 2.53); antennal scrobes-frons shallow. Face convex with scattered finely punctate, interspaces smooth, and longitudinal median carina present. Frons smooth. Temple wide, punctate, and interspaces clearly smooth. Inner margin of eyes straight throughout; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.09, 0.12). Malar suture present. Median area between lateral ocelli without depression. Vertex laterally rounded and dorsally wide. Mesosoma (Fig. 31A, G, H, J). Mesosoma dorsoventrally convex. Mesoscutum convex, punctation distinct throughout, and interspaces smooth. Scutellum long and slender, apex sloped and fused with BS, scutellar punctation indistinct throughout, scutellum in profile flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM; ATS demilune with a little and complete parallel carinae; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and heterogeneous foveae; area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM upward; MPM circular without median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM; PFM thick and smooth; ATM proximally with little and incomplete parallel carinae. Propodeum with a clearly visible median longitudinal carina, proximal half straight or nearly so, with fine sculpture and distal half rugose with a shallow dent at each side of nucha; distal edge of propodeum with a flange at each side and short stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron finely sculptured only ventrally and dorsally without a carina. Metasternum flat or nearly so. Contour of mesopleuron convex; precoxal groove smooth, shiny and shallow, but visible; epicnemial ridge convex, teardrop-shaped. Legs (Fig. 31A, E). Ventral margin of fore telotarsus slightly excavated and with a tiny curved seta, fore telotarsus proximally narrow and distally wide, and longer than fourth tarsomere (0.12, 0.06). Hind coxa finely punctate throughout, and dorsal outer depression absent. Inner spur of hind tibia longer than outer spur (0.24, 0.15); entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus as equal as fourth tarsomere (0.11, 0.10). Wings (Fig. 31L, M). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, complete, and touching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally and subproximally evenly convex, and setae evenly scattered in the margin. Metasoma (Fig. 31A, F, I, K). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, evenly narrowing distally and apex truncate (length 0.39, maximum with 0.22, minimum width 0.08) with pubescence on distal half. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.22, length T2 0.22), edges of median area polished and lateral grooves deep, median area longer than broad (length 0.22 mm, maximum width 0.18, minimum width 0.08); T2 scarce pubescence throughout. T3 longer than T2 (0.25, 0.22) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. White bud-like cocoon with body ridge-shaped and silk fibers evenly smooth. Cocoons scattered loose and adhered to the leaf substrate. Comments. The shape of pronotum is characteristic, the distal half is convex instead of concave. The propodeum with an incomplete median longitudinal carina. Male (Fig. 32 A–M). Similar in coloration to females. Males tend to be slenderer than females. Etymology. Robert (Bob) Hanner is working as Associate Director at Canadian Barcode of Life Network at the Biodiversity Institute of Ontario (BIO), University of Guelph, Ontario, Canada. Distribution. The parasitized caterpillars were collected in Costa Rica, ACG, Sector Pitilla (Estación Quica, Sendero Laguna, and Sendero Mismo), during June-July 2004 and July 2008 at 680 m in rain forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Scotura leucophleps Warren (Notodontidae: Dioptinae) feeding on Rinorea deflexiflora and R. sylvatica (Violaceae). Caterpillars were collected in third and fourth instar., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles grantgentryi Arias-Penna, sp. nov.Fig. 94 Female. Body length 2.88 mm, antenna length 3.58 mm, fore wing length 3.33 mm. Type material. Holotype: ECUADOR • 1♀; EC-2645, YY-A147; Napo, Yanayacu Biological Station, Yanayacu Road; cloud forest; 2,100 m; - 0.566667, -77.866667; 03.v.2005; Harold Greeney leg.; cocoon formed on 16.v.2005; adult parasitoids emerged on 27.v.2005; (PUCE). Paratypes. • 1 (0♀, 1♂) (0♀, 0♂); EC-2645, YY-A147; same data as for holotype; (PUCE). 3 (1♀, 1♂) (0♀, 1♂); EC-2644, YY-A073; same data as for holotype except: cocoons formed on 26.v.2005; (PUCE). Diagnosis. Vertex in lateral view pointed (Fig. 94C), frons smooth, scutellar punctation indistinct throughout (Fig. 94E), in lateral view, metasoma laterally compressed (Fig. 94A), median area on T2 broader than long (Fig. 94G), lateral grooves delimiting the median area on T2 distally losing definition on T2 (Fig. 94G), petiole on T1 parallel-sided in proximal half, then narrowing (Fig. 94G, H) and finely sculptured (Fig. 94G), propodeum without a median longitudinal dent (Fig. 94F), and fore wing with r vein straight, outer side of junction of r and 2RS veins forming a stub (Fig. 94K). Coloration (Fig. 94 A–L). General body coloration brown-black, scape and all antennal flagellomeres (on both sides) brown; pedicel brown with an distal narrow yellow-brown ring; labrum light brown; mandible yellow-brown; glossa, maxillary and labial palps, and tegulae yellow; propleuron light brown with a tiny yellow-brown distal-ventral spot; dorsal and ventral furrows of pronotum somewhat lighter than mesosoma coloration. Eyes and ocelli silver. Fore and middle legs yellow except brown claws; hind legs yellow except black coxae with apex yellow, femora with a tiny brown area at the apex, tibiae with both ends brown, and tarsomeres brown, although basitarsus with a proximal yellow ring. Petiole on T1 black and sublateral ends yellow; T2 with median area black, adjacent area brown and lateral ends yellow with two elongate spots one on each side; T3 mostly brown although lateral ends with proximal half yellow/yellow-brown; T4 and beyond completely black; distally each tergum with a narrow yellow/whitish transparent band. In lateral view, T1-2 completely yellow; T3-4 yellow, but dorsally brown; T5 and beyond completely dark brown. S1 proximal half brown, distal half yellow; S2-3 completely yellow; S4 yellow, medially brown; penultimate sternum and hypopygium completely brown. Description. Head (Fig. 94 A–D). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.24:0.06, 0.26:0.06, 0.25:0.06), distal antennal flagellomere longer than penultimate (0.15:0,05, 0.10:0.05), antenna longer than body (3.58, 2.88); antennal scrobes-frons shallow. Face flat or nearly so with dense fine punctations, interspaces smooth and longitudinal median carina present. Frons smooth. Temple wide, punctate, interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.09, 0.12). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma (Fig. 94A, E, F, I). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct throughout, interspaces smooth. Scutellum triangular, apex sloped and fused with BS, scutellar punctation indistinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS not overlapping the MPM; ATS demilune with quite a little complete parallel carinae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation wih large and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and nearly at the same level as mesoscutum (flat). Metanotum with BM wider than PFM (clearly differentiated); MPM circular without median longitudinal carina; AFM with a small lobe and not as well delineated as PFM; PFM thick and smooth; ATM proximally with semircular/undulate carina and distally smooth. Propodeum without median longitudinal carina, proximal half weakly curved with medium-sized sculpture and distal half slightly rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron finely sculptured only ventrally and dorsally without a carina. Metasternum flat or nearly so. Contour of mesopleuron straight/angulate or nearly so; precoxal groove smooth, shiny and distinct; epicnemial ridge convex, teardrop-shaped. Legs. Ventral margin of fore telotarsus excavated with conspicuous curved seta over this excavation, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.15, 0.09). Hind coxa with very finely punctate throughout, and dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.25, 0.21), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.23, 0.12). Wings (Fig. 94K, L). Fore wing with r vein straight; 2RS vein straight; r and 2RS veins forming an angle at their junction and outer side of junction forming a slight stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a straight, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma (Fig. 94A, G, H, J). Metasoma laterally compressed. Petiole on T1 finely sculptured throughout, parallel-sided in proximal half and then narrowing (length 0.37, maximum width 0.19, minimum width 0.09), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.18, length T2 0.18), edges of median area with little sculpture, median area broader than long (length 0.18, maximum width 0.25, minimum width 0.07); T2 with scattered pubescence only distally. T3 longer than T2 (0.20, 0.18) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. Unknown. Comments. In females the hypopygium (S6) is the only sternum that is completely dark, penultimate sternum (S5) is yellow-brown. Male. The coloration is similar to female, except that colored adjacent area on T2 is not extensive and the two elongate spots are not noticeable, and besides T3 and beyond are completely brown. The body coloration is slightly darker than females and the hind tibia looks completely dark. Etymology. Grant Gentry is an American biologist with interests in tritrophic interactions, tropical caterpillars, and efficacies of lepidopteran larval defenses against parasitoids, with an emphasis on chemical defenses derived from food plants. He works at Samford University, Birmingham, AL, USA. Distribution. Parasitized caterpillar was collected in Ecuador, Napo, Yanayacu Biological Station (Yanayacu Road), during May 2005 at 2,100 m in cloud forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Undetermined species of Notodontidae feeding on Myriocarpa sp. (Urticaceae). Caterpillar instar was not reported., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles howelldalyi Arias-Penna, sp. nov.Figs 101, 102 Female. Body length 2.68 mm, antenna length 2.63 mm, fore wing length 2.53 mm. Type material. Holotype: COSTA RICA • 1♀; 08-SRNP-72188, DHJPAR0031040; Área de Conservación Guanacaste, Alajuela, Sector Pitilla, Medrano; rain forest; 380 m; 11.01602, -85.38053; 26.viii.2008; Walter Siezar leg.; caterpillar collected in fourth instar; two rows of cordwood cocoons adhered to larva and substrate, cocoons formed on 02.ix.2008; adult parasitoids emerged on 09.ix.2008; (CNC). Paratypes. • 69 (1♀, 2♂) (37♀, 29♂); 08-SRNP-72188, DHJPAR0031040; same data as for holotype; (CNC). Other material. Reared material. COSTA RICA: Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Potrero Argentina: • 59 (3♀, 3♂) (44♀, 9♂); 03-SRNP-9142, DHJPAR0000048; pastures; 520 m; 10.89021, -85.38803; 11.x.2003; Elda Araya leg.; caterpillar collected in fourth instar; mass of brown cocoons tightly glued to each other and to the leaf, forming two rows of cordwood, a bit irregular, caterpillar must has been on the side; adult parasitoids emerged on 14.x.2003. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Estación San Cristobal : • 38 (3♀, 3♂) (27♀, 5♂); 04-SRNP-3377, DHJPAR0000284; rain forest; 640 m; 10.87097, -85.39144; 10.vii.2004; Elda Araya leg.; caterpillar collected in fourth instar; two rows of cordwood cocoons adhered to the leaf substrate; adult parasitoids emerged on 17.vii.2004. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Corrales viejos: • 78 (3♀, 3♂) (40♀, 32♂); 04-SRNP-4458, DHJPAR0000277; rain forest; 495 m; 10.89974, -85.38085; 31.viii.2004; Osvaldo Espinoza leg.; caterpillar collected in fifth instar; two rows of cordwood cocoons adhered to the leaf substrate; adult parasitoids emerged on 12.ix.2004. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Quebrada Cementerio: • 38 (3♀, 3♂) (23♀, 9♂); 06-SRNP-5536, DHJPAR0012096; rain forest; 700 m; 10.87124, -85.38749; 08.vii.2006; Elda Araya leg.; caterpillar collected in fourth instar; two somewhat irregular rows of cordwood on each side of the cadaver, cocoons form on 19.vii.2006; adult parasitoids emerged on 26.vii.2006. Alajuela, Dos Ríos, Sector San Cristóbal, Finca San Gabriel: • 51 (4♀, 3♂) (32♀, 12♂); 03-SRNP-34135, DHJPAR0000275, DHJPAR0001529; rain forest; 645 m; 10.87766, -85.39343; 10.xi.2003; Carolina Cano leg.; caterpillar collected in fifth instar; very dense two rows of cordwood brown cocoons, but not on each side of the caterpillar but along the midrib of the leaf; adult parasitoids emerged on 18.xi.2003. • 24 (3♀, 3♂) (15, 3♂); 04-SRNP-4174, DHJPAR0001479; same data as for preceding except: 20.viii.2004; Yessenia Mendoza leg.; caterpillar collected in third instar; cocoons adhered to the leaf substrate; adult parasitoid emerged on 14.ix.2004. • 26 (3♀, 3♂) (19♀, 1♂); 07-SRNP-2795, DHJPAR0020267; same data as for preceding except: 20.vi.2007, Minor Carmona leg.; caterpillar collected in third instar; cocoons adhered to the leaf substrate; adult parasitoid emerged on 06.vii.2007. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Río Blanco Abajo : • 103 (3♀, 3♂) (89♀, 8♂); 04-SRNP-1182, DHJPAR0020497; rain forest; 500 m; 10.90037, -85.37254; 04.iv.2004; Osvaldo Espinoza leg.; caterpillar collected in fifth instar; two rows of cordwood brown cocoons adhered to the leaf substrate; adult parasitoids emerged on 09.iv.2004. • 56 (3♀, 3♂) (27♀, 23♂); 06-SRNP-5231, DHJPAR0012009; same data as for preceding except: 28.vi.2006; Anabelle Córdoba leg.; caterpillar collected in fourth instar; cocoons adhered to the leaf substrate, cocoon characteristics not reported; adult parasitoids emerged on 12.vii.2006. • 45 (3♀, 3♂) (34♀, 5♂); 06-SRNP-5232, DHJPAR0012012; same data as for preceding except: 28.vi.2006; Anabelle Córdoba leg.; caterpillar collected in fourth instar; adult parasitoids emerged on 11.vii.2006. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Puente Palma: • 21 (3♀, 0♂) (18♀, 0♂); 03-SRNP-9723, DHJPAR0000274; rain forest; 460 m; 10.9163, -85.37869; 30.x.2003; Gloria Sihezar leg.; caterpillar collected in fourth instar; row of brown cordwood cocoons on each side of the cadaver; adult parasitoids emerged on 08.xi.2003. • 84 (3♀, 3♂) (74♀, 4♂); 04-SRNP-4137, DHJPAR0001466; same data as for preceding except: 19.viii.2004; Yessenia Mendoza leg.; caterpillar collected in third instar; two rows of stacked cordwood cocoons adhered to the leaf substrate; adult parasitoids on 02.ix.2004. • 59 (3♀, 3♂) (49♀, 4♂); 04-SRNP-4138, DHJPAR0001461; same data as for preceding except: 19.viii.2004; Yessenia Mendoza leg.; caterpillar collected in third instar; two rows of cordwood cocoons adhered to the leaf substrate; adult parasitoids on 02.ix.2004. • 104 (3♀, 3♂) (90♀, 8♂); 04-SRNP-4139, DHJPAR0000282; same data as for preceding except: 19.viii.2004; Yessenia Mendoza leg.; caterpillar collected in third instar; two rows of stacked cordwood adhered to the leaf substrate; adult parasitoids emerged on 02.ix.2004. • 54 (3♀, 3♂) (41♀, 7♂); 04-SRNP-4801, DHJPAR0001478; same data as for preceding except: 24.ix.2004; Osvaldo Espinoza leg.; caterpillar collected in third instar; two rows of cordwood cocoons adhered to the leaf substrate; adult parasitoids emerged on 13.x.2004. • 66 (3♀, 3♂) (51♀, 9♂); 04-SRNP-4804, DHJPAR0001469; same data as for preceding except: 24.ix.2004; Osvaldo Espinoza leg.; caterpillar collected in third instar; two rows of brown cordwood cocoons adhered to the leaf substrate; adult parasitoids emerged on 09.x.2004. • 63 (3♀, 3♂) (53♀, 4♂); 04-SRNP-4814, DHJPAR0000279; same data as for preceding except: 24.ix.2004; Osvaldo Espinoza leg.; caterpillar collected in third instar; cordwood cocoons on both sides of cadaver, cocoons adhered to the leaf substrate; adult parasitoids emerged on 09.x.2004. • 47 (3♀, 3♂) (37♀, 4♂); 05-SRNP-6986, DHJPAR0004772; same data as for preceding except: 06.xi.2005; Yessenia Mendoza leg.; two rows of cordwood cocoons adhered to the leaf substrate; adult parasitoids emerged on 20.xi.2005. • 41 (3♀, 3♂) (30♀, 5♂); 05-SRNP-6987, DHJPAR0004778; same data as for preceding except: 06.xi.2005; Yessenia Mendoza leg.; two rows of cordwood cocoons adhered to the leaf substrate; adult parasitoids emerged on 20.xi.2005. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Vado Río Cucaracho : • 24 (3♀, 3♂) (15♀, 3♂); 99-SRNP-5745, DHJPAR0001520; rain forest; 640 m; 10.8702, -85.39153; 08.vi.1999; Gloria Sihezar leg.; caterpillar collected in fourth instar; gray cordwood cocoons adhered to the leaf substrate; adult parasitoids emerged on 12.vi.1999. • 52 (3♀, 3♂) (46♀, 0♂); 06-SRNP-5588, DHJPAR0012117; same data as for preceding except: 11.vii.2006; cocoons adhered to the leaf substrate; adult parasitoids emerged on 24.vii.2006. • 60 (3♀, 3♂)(36♀, 18♂); 06-SRNP-5589, DHJPAR0012115; 11.vii.2006; cocoon characteristics not reported; adult parasitoids emerged on 22.vii.2006. • 42 (3♀, 2♂) (37♀, 0♂); 06-SRNP-5590, DHJPAR0012109; same data as for preceding except: 11.vii.2006; caterpillar collected in third instar; two rows of cordwood cocoons with caterpillar in between; adult parasitoids emerged on 30.vii.2006. • 24 (3♀, 3♂) (3♀, 15♂); 06-SRNP-5591, DHJPAR0012097; same data as for preceding except: 11.vii.2006; caterpillar collected in third instar; larval caterpillar is still alive between the two rows of cordwood cocoons; adult parasitoids emerged on 28.vii.2006. • 60 (3♀, 3♂) (42♀, 12♂); 06-SRNP-5592, DHJPAR0012116; same data as for preceding except: 11.vii.2006; caterpillar collected in third instar; brown cocoons arranged in two rows of cordwood; adult parasitoids emerged on 31.vii.2006. Área de Conservación Guanacaste , Guanacaste, Sector Cacao, Puente Gongora: • 47 (3♀, 2♂) (42♀, 0♂); 06-SRNP-46302, DHJPAR0012108; cloud forest; 540 m; 10.88489, -85.47203; 23.vii.2006; Harry Ramirez leg.; caterpillar collected in fifth instar; brown cordwood cocoons adhered to the leaf substrate, cocoons formed on 27.vii.2006; adult parasitoids emerged on 03.viii.2006. Área de Conservación Guanacaste , Guanacaste, Sector Pitilla, Coneja: • 12 (3♀, 2♂) (7♀, 0♂); 08-SRNP-32254, DHJPAR0031042; rain forest; 415 m; 11.01525, -85.39766; 08.ix.2008; Manuel Rios leg.; caterpillar collected in fifth instar, cocoons already present and adhered to the leaf substrate; adult parasitoids emerged on 15.ix.2008. • 59 (3♀, 3♂) (59♀, 4♂); 08-SRNP-32255, DHJPAR0031039; same data as for preceding except: Calixto Moraga leg.; caterpillar collected in fourth instar, two rows of cordwood cocoons already present and adhered to the leaf substrate; adult parasitoids emerged on 16.ix.2008. Área de Conservación Guanacaste , Guanacaste, Sector Pitilla, Estación Quica : • 104 (3♀, 3♂) (38♀, 60♂); 08-SRNP-71793, DHJPAR0031105; rain forest; 470 m; 10.99697, -85.39666; 21.vii.2008; Leonel Siezar leg.; caterpillar collected in fifth instar; two rows of brown cordwood cocoons already present and adhered to the leaf substrate; adult parasitoids emerged on 26.vii.2008. • 97 (3♀, 3♂) (33♀, 58♂); 08-SRNP-71803, DHJPAR0031096; same data as for preceding except: 25.vii.2008; caterpillar collected in fourth instar; brown cordwood cocoons adhered to the leaf substrate and formed on 03.viii.208; adult parasitoids emerged on 11.viii.2008. • 96 (3♀, 3♂) (74♀, 16♂); 08-SRNP-72135, DHJPAR0031033; same data as for preceding except: 25.viii.2008; double cordwood cocoons adhered to the leaf substrate and formed on 02.ix.2008; adult parasitoids emerged on 08.ix.2008. • 63 (3♀, 3♂) (27♀, 30♂); 08-SRNP-72136, DHJPAR0031027; same data as for preceding except: 25.viii.2008; Ronald Siezar leg.; double cordwood cocoons adhered to the leaf substrate and formed on 05.ix.2008; adult parasitoids emerged on 11.ix.2008. • 64 (3♀, 3♂) (54♀, 4♂); 08-SRNP-72138, DHJPAR0031026; same data as for preceding except: 25.viii.2008; Marta Acosta leg.; two rows of brown cordwood cocoons adhered to the leaf substrate and formed on 26.viii.2008; adult parasitoids emerged on 02.ix.2008. • 135 (3♀, 3♂) (109♀, 20♂); 08-SRNP-72383, DHJPAR0031030; same data as for preceding except: 13.ix.2008; Walter Siezar leg.; cocoons adhered to the leaf substrate and formed on 17.ix.2008; cocoon characteristics not reported; adult parasitoids emerged on 25.ix.2008. • 121 (3♀, 3♂) (96♀, 59♂); 08-SRNP-72384, DHJPAR0031041; same data as for preceding except: 13.ix.2008; two rows of cordwood cocoons adhered to the leaf substrate and formed on 17.ix.2008; adult parasitoids emerged on 23.ix.2008. • 106 (3♀, 3♂) (27♀, 73♂); 08-SRNP-72385, DHJPAR0031034; same data as for preceding except: 13.ix.2008; two rows of cordwood cocoons adhered to the leaf substrate and formed on 18.ix.2008; adult parasitoids emerged on 24.ix.2008. • 134 (3♀, 3♂) (65♀, 62♂); 08-SRNP-72386, DHJPAR0031021; same data as for preceding except: 13.ix.2008; Marta Acosta leg.; two rows of cordwood cocoons adhered to the leaf substrate and formed on 17.ix.2008; adult parasitoids emerged on 23.ix.2008. • 48 (3♀, 3♂) (39♀, 3♂); 09-SRNP-70451, DHJPAR0035403; same data as for preceding except: 19.v.2009; Calixto Moraga leg.; mass of very ordered cocoons on leaf below alive caterpillar; adult parasitoids emerged on 25.v.2009. • 56 (5♀, 5♂) (42♀, 4♂); 09-SRNP-70452, DHJPAR0035427; same data as for preceding except: 19.v.2009; Ricardo Calero leg.; row of cordwood cocoons on each side of alive caterpillar; adult parasitoids emerged on 24.v.2009. Área de Conservación Guanacaste , Guanacaste, Sector Pitilla, Medrano: • 19 (3♀, 3♂) (13♀, 0♂); 08-SRNP-72115, DHJPAR0031020; rain forest; 380 m; 11.01602, -85.38053; 20.viii.2008; Leonel Siezar leg.; caterpillar collected in fourth instar; cocoons adhered to the leaf substrate and formed on 29.viii.2008; adult parasitoids emerged on 06.ix.2008. • 100 (3♀, 3♂) (89♀, 5♂); 08-SRNP-72116, DHJPAR0031035; same data as for preceding except: Marta Acosta leg.; two rows of cordwood cocoons adhered to the leaf substrate and formed on 02.ix.2008; adult parasitoids emerged on 09.ix.2008. • 68 (3♀, 3♂) (60♀, 2♂); 08-SRNP-72120, DHJPAR0031036; same data as for preceding except: Marta Acosta leg.; two rows of brown cordwood cocoons; adult parasitoids emerged on 05.ix.2008. • 48 (3♀, 3♂) (38♀, 4♂); 08-SRNP-72184, DHJPAR0031038; same data as for preceding except: 26.viii.2008; Walter Siezar leg.; two rows of cordwood cocoons adhered to the leaf substrate and formed on 02.ix.2008. • 88 (3♀, 3♂) (58♀, 24♂); 08-SRNP-72185, DHJPAR0031031; same data as for preceding except: 26.viii.2008; Walter Siezar leg.; two rows of cordwood cocoons adhered to the leaf substrate and formed on 02.ix.2008; adult parasitoids emerged on 08.ix.2008. • 85 (3♀, 3♂) (67♀, 12♂); 08-SRNP-72186, DHJPAR0031028; same data as for preceding except: 26.viii.2008; Walter Siezar leg.; two rows of cordwood cocoons adhered to the leaf substrate and formed on 02.ix.2008; adult parasitoids emerged on 07.ix.2008. • 27 (3♀, 3♂) (7♀, 14♂); 08-SRNP-72187, DHJPAR0031043; same data as for preceding except: 26.viii.2008; Walter Siezar leg.; two rows of cordwood cocoons adhered to the leaf substrate and formed on 06.ix.2008; adult parasitoids emerged on 10.ix.2008. • 78 (3♀, 3♂) (46♀, 26♂); 08-SRNP-72189, DHJPAR0031024; same data as for preceding except: 26.viii.2008; Walter Siezar leg.; double cordwood cocoons adhered to the leaf substrate and formed on 02.ix.2008; adult parasitoids emerged on 07.ix.2008. • 109 (3♀, 3♂) (95♀, 18♂); 08-SRNP-72190, DHJPAR0031025; same data as for preceding except: 26.viii.2008; Walter Siezar; two rows of cordwood cocoons adhered to the leaf substrate and formed on 02.ix.2008; adult parasitoids emerged on 08.ix.2008. • 75 (3♀, 3♂) (45♀, 24♂); 08-SRNP-72192, DHJPAR0031022; same data as for preceding except: 26.viii.2008; Walter Siezar; caterpillar collected in third instar; two rows of cordwood cocoons adhered to the leaf substrate and formed on 10.ix.2008; adult parasitoids emerged on 15.ix.2008. • 104 (3♀, 3♂) (28♀, 70♂); 08-SRNP-72194, DHJPAR0031032; same data as for preceding except: 26.viii.2008; Walter Siezar leg.; caterpillar collected in third instar; two rows of cordwood cocoons adhered to the leaf substrate and formed on 10.ix.2008; adult parasitoids emerged on 15.ix.2008. Diagnosis. Mesoscutum punctate throughout (Figs 101B, 102B), phragma of the scutellum partially exposed (Figs 101C, 102C), ntenna shorter than body, ventral margin of fore telotarsus slightly excavated, scutellar punctation indistinct throughout (Figs 101B, 102B), propodeal spiracle without distal carina (Figs 101C, 102C), petiole on T1 distally with lateral margins relatively straight, finely sculptured only laterally (Figs 101D, G, 102D, G), surface of metasternum flat or nearly so, precoxal groove deep with lineate sculpture (Figs 101A, E, 102A, E), fore wing with vein 1 cu-a curved, r vein curved, outer side of junction of r and 2RS veins not forming a stub (Figs 101J, 102I), dorsal outer depression on hind coxa present (Figs 101A, F, 102A, F), inner margin of eyes diverging slightly at antennal sockets, propodeum without median longitudinal carina (Figs 101C, 102C), lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (Figs 101D, G, 102D, G). Coloration (Fig. 101A). General body coloration polished brown-black except labrum and mandibles yellow-brown; glossa, maxillary and labial palps and tegulae yellow; scape and pedicel yellow, but laterally brown; three most proximal antennal flagellomeres dorsally lighter (yellow-brown) than ventrally (dark brown), remaining flagellomeres dark brown on both sides. Eyes and ocelli silver. Fore and middle legs yellow except coxae and claws brown-black; hind legs yellow except coxae, apex of femora and most part of the tibiae brown-black, tarsomeres brown except proximal half of basitarsus. Petiole on T1 black, but middle yellow and sublateral areas yellow; T2 with median and very narrow adjacent areas black and lateral ends yellow; T3 black, but proximal corners yellow and distal corners with one oval yellow spot at each side; T3 and beyond brown; distally each tergum with a narrow transparent band. In lateral view, T1-2 completely yellow; T3 yellow, dorsally dark brown; T4 and beyond completely brown. S1-2 completely yellow; S3 yellow distally with a narrow brown band; S4 proximally with a narrow yellow band, distally with a wide brown band; penultimalte sternum and hypopygium completely brown. Description. Head (Fig. 101A, B, E). Head triangular with pubescence short and dense. Proximal three antennal flagellomeres longer than wide (0.21:0.06, 0.20:0.06, 0.20:0.06), distal antennal flagellomere longer than penultimate (0.13:0.06, 0.09:0.06), antenna shorter than body (2.63, 2.68); antennal scrobes-frons shallow. Face flat or nearly so, with dense fine punctations, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.13). Malar suture present. Median area between lateral ocelli without depression. Vertex laterally rounded and dorsally wide. Mesosoma (Fig. 101 A–C, E). Mesosoma dorsoventrally convex. Distal 1/3 of mesoscutum with lateral margin slightly dented, punctation distinct throughout, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS, scutellar punctation indistinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM; ATS demilune with short stubs delineating the area; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM wider than PFM (clearly differentiated); MPM circular and bisected by a median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM; PFM thick and smooth; ATM proximally with semircular/undulate carina and distally smooth. Propodeum without median longitudinal carina, proximal half curved and relatively polished and distal half relatively polished; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with a mix of rugae and fine punctation, dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron straight/angulate or nearly so; precoxal groove deep with faintly lineate sculpture; epicnemial ridge elongated more fusiform (tapering at both ends). Legs. Ventral margin of fore telotarsus slightly excavated and with a tiny curved seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.12, 0.08). Hind coxa with punctation only on ventral surface and dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.26, 0.19), entire surfac, Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles kevinjohnsoni Arias-Penna, sp. nov.Fig. 139 Female. Body length 2.83 mm, antenna length 3.28 mm, fore wing length 3.43 mm. Type material. Holotype: ECUADOR • 1♀; EC-38518, YY-A004; Napo, Yanayacu Biological Station, Yanayacu Road; cloud forest; 2,100 m; - 0.566667, -77.866667; 30.iv.2009; CAPEA leg.; caterpillar collected in second instar; cocoons formed on 05.vi.2009; adult parasitoids emerged on 26.vi.2009; (PUCE). Paratypes. • 73 (9♀, 5♂) (59♀, 0♂); EC-38518, YY-A004; same data as for holotype; (PUCE). Other material. Reared material. ECUADOR: Napo, Yanayacu Biological Station, Yanayacu Road: • 83 (6♀, 6♂) (62♀, 9♂); EC-2807, YY-A083; cloud forest; 2,100 m; - 0.566667, -77.866667; 22.v.2005; CAPEA leg.; adult parasitoids emerged on 12.vii.2005. Diagnosis. Propleuron finely sculptured only ventrally (Fig. 139A, C), longitudinal median carina on face absent (Fig. 139B), surface of metasternum convex, edges of median area on T2 obscured by weak longitudinal stripes (Fig. 139G, H), dorsal outer depression on hind coxa absent (Fig. 139A, J), and fore wing with r vein slightly curved or curved, outer side of junction of r and 2RS veins forming a slight or distinct stub (Fig. 139K). Coloration (Fig. 139 A–K). General body coloration polished black except scape and all antennal flagellomeres (on both sides) dark brown; pedicel, labrum and mandibles brown-red/reddish; glossa, maxillary and labial palps, and tegulae yellow; lunules, BS, PFM and BS with brown-red/reddish tints. Eyes silver and ocelli yellowish. Fore and middle legs yellow except brown claws and tarsomeres with brown tints; hind legs yellow except black coxae with apex yellow, femora with brown apex, distal half of tibiae brown and distally with a small brown band, and tarsomeres brown. Petiole on T1 black, contours darkened and sublateral areas yellow; T2 with median and wide adjacent areas brown, and lateral ends yellow-brown; T3 brown except a small yellow-brown area in proximal corners; T4 and beyond brown; distally each tergum with a narrow whitish translucent band. In lateral view, T1-2 yellow; T3-4 yellow, but dorsally yellow-brown, the extent of yellow-brown area increasing from proximal to distal; T5 and beyond completely brown. S1-2 yellow; S3 proximal half yellow, distal half brown; S4 and beyond brown. Description. Head (Fig. 139 A–D). Head elongate with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.26:0.09, 0.28:0.09, 0.25:0.09), distal antennal flagellomere longer than penultimate (0.13:0.05, 0.10:0.07), antenna longer than body (3.28, 2.83); antennal scrobes-frons sloped and forming a shelf. Face fine and punctate-lacunose, interspaces wavy and with lateral depression only middle, and longitudinal median carina absent. Frons smooth. Temple wide, punctations barely noticeable and interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.08, 0.13). Malar suture absent or difficult to see. Median area between lateral ocelli without depression. Vertex laterally rounded and dorsally wide. Mesosoma (Fig. 139A, E, F, I). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct throughout, interspaces smooth. Scutellum long and slender, apex sloped and fused with BS, but not in the same plane, scutellar punctation scattered throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS not overlapping the MPM; ATS demilune only inner side with sculpture; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation nearly at the same level as mesoscutum (flat) and with same kind of sculpture as mesoscutum. Metanotum with BM wider than PFM (clearly differentiated); MPM circular with some sculpturing inside; AFM without setiferous lobes and not as well delineated as PFM; PFM thick, smooth and with lateral ends rounded; ATM proximally with sculpture distally without a well delimited smooth area. Propodeum with a median longitudinal dent, but no trace of median longitudinal carina, proximal half weakly curved with medium-sized sculpture and distal half slightly rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by faintly concave/wavy carina; nucha surrounded by long radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum and dorsal furrow smooth, but ventral furrow with short parallel carinae. Propleuron finely sculptured only ventrally and dorsally with a carina. Metasternum convex. Contour of mesopleuron convex; precoxal groove smooth, shiny and shallow, but visible; epicnemial ridge convex, teardrop-shaped. Legs. Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.14, 0.12). Hind coxa finely punctate throughout, and dorsal outer depression absent. Inner spur of hind tibia longer than outer spur (0.30, 0.21), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus as equal in length as fourth tarsomere (0.18, 0.17). Wings (Fig. 139K). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction forming a slight stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a straight, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma (Fig. 139A, G, H, J). Metasoma laterally compressed. Petiole on T1 with a mix of fine rugae and punctate sculpture over most of the surface, virtually parallel-sided over most of length, but barely narrowing over distal 1/3, apex truncate (length 0.41, maximum width 0.19, minimum width 0.11), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.21, length T2 0.21), edges of median area obscured by weak longitudinal stripes, median area broader than long (length 0.21, maximum width 0.23, minimum width 0.09); T2 with scattered pubescence throughout. T3 longer than T2 (0.24, 0.21) and with pubescence more notorious in distal half. Pubescence on hypopygium dense. Cocoons. Unknown. Comments. In some specimens of the same sample, the coloration of sterna is a little different, distally all are brown with a longitudinal yellow band; the middle coxae proximally with a dorsal brown spot. In some specimens (e.g., EC-2807), the pronotum and the propleuron with brown-red/reddish tints. The malar space in this species is wide. The area between antennal scrobes is dented. The shape of the body is very elongated. Male. Similar in coloration to females. The same color variation found in females are also present in males. Etymology. Kevin P. Johnson is an American biologist. His major fields of interest are avian and insect systematics, host-parasite coevolution, island biogeography, population genetics, and behavioral ecology. He works at the Illinois Natural History Survey, Champaign, IL, USA. Distribution. Parasitized caterpillars were collected in Ecuador, Napo, Yanayacu Biological Station (Yanayacu Road), during May 2005 and April 2009 at 2,100 m in cloud forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Undetermined species of Erebidae (Arctiinae) feeding on Rubus sp. (Rosaceae). Caterpillar was collected in second instar., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles yanayacuensis Arias-Penna, sp. nov.Fig. 223 Female. Body length 2.83 mm, antenna length 3.53 mm, fore wing length 3.33 mm. Type material. Holotype: ECUADOR • 1♀; EC-41685, YY-A154; Napo, Yanayacu Biological Station, Stream trail, Plot 439; cloud forest; 2,114 m; - 0.596944, -77.888333; 18.viii.2009; Lee Dyer leg.; caterpillar collected in third instar; cocoon formed on 28.viii.2009; adult parasitoid emerged on 24.ix.2009; (PUCE). Diagnosis. Petiole on T1 completely smooth and polished, with faint, satin-like sheen (Fig. 223G, H), vertex in lateral view pointed or nearly so (Fig. 223C), scutellar punctation scattered throughout (Fig. 223F), phragma of the scutellum partially exposed (Fig. 223F), median area on T2 broader than long, lateral grooves delimiting the median area clearly defined and reaching the distal edge of T2, edges of median area polished and followed by a deep groove (Fig. 223G, H), anterior furrow of metanotum with a small lobe, without setae (Fig. 223F), axillary trough of scutellum almost smooth (Fig. 223F), propodeum without median longitudinal carina (Fig. 223F), anteroventral contour of mesopleuron convex (Fig. 223A, I), and fore wing with r vein curved, outer side of junction of r and 2RS veins forming a slight stub (Fig. 223K). Coloration (Fig. 223 A–L). General body coloration black except labrum, mandibles, glossa, and tegulae yellow-brown; scape and pedicel yellow-brown, but laterally brown; first four-five proximal antennal flagellomeres dorsally lighter (light brown) than ventrally (dark brown), remaining flagellomeres dark brown on both sides; maxillary and labial palps pale yellow/ivorish; apex of propleuron yellow; clypeus, area just below antennal scrobes, pronotum, epicnemial ridge, ventral edge of mesopleuron, distal corners of mesoscutum, lunules, BS, PFM, and lateral ends of metapleuron with brown-red/reddish tints. Eyes silver and ocelli yellowish. Fore and middle legs light yellow except brown claws; hind legs light yellow except femora distally with tiny a dorsal brown spot, distal 1/3 of tibiae brown and proximally with a tiny brown area, and tarsomeres brown although basitarsus proximal half yellow. Petiole on T1 brown-red/reddish, contours darkened and sublateral areas yellow; T2 with median and adjacent areas brown-red/reddish, adjacent area with contours well-defined, both dark areas forming a rectangle-shaped area, and lateral ends yellow; T3 mostly brown, that dark area coinciding with the width of dark area formed by both median and adjacent areas on T2, and lateral ends narrow and yellow; T4 and beyond completely brown; distally each tergum with a narrow whitish transparent band. In lateral view, T1-3 yellow; T4 and beyond brown. S1-3 yellow; S4 yellow, but medially brown; penultimate sternum and hypopygium brown. Description. Head (Fig. 223 A–D). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.26:0.07, 0.28:0.07, 0.28:0.07), distal antennal flagellomere longer than penultimate (0.15:0.06, 0.12:0.06), antenna longer than body (3.53, 2.83); antennal scrobes-frons sloped and forming a shelf. Distal half of face dented laterally, finely punctate-lacunose, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate and interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.13). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma (Fig. 223A, F, I). Mesosoma dorsoventrally convex. Mesoscutum poximally convex and distally flat, punctation distinct proximally, but absent/dispersed distally, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS, but not in the same plane, scutellar punctation scattered throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM; ATS demilune almost smooth; dorsal ATS groove smooth. Transscutal articulation with small and homogeneous foveae, area just behind transscutal articulation nearly at the same level as mesoscutum (flat), smooth and shiny. Metanotum with BM wider than PFM (clearly differentiated); MPM circular without median longitudinal carina; AFM with a small lobe and not as well delineated as PFM; PFM thick, smooth and with lateral ends rounded; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum without median longitudinal carina, proximal half weakly curved with fine sculpture and distal half relatively polished and with a shallow dent at each side of nucha; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by faintly concave/wavy carina; nucha surrounded by very short radiating carinae. Pronotum with a faint dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum and dorsal furrow smooth, but ventral furrow with short parallel carinae. Propleuron with fine punctations throughout and dorsally without a carina. Metasternum convex. Contour of mesopleuron convex; precoxal groove deep, smooth and shiny; epicnemial ridge convex, teardrop-shaped. Legs (Fig. 223A). Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.13, 0.08). Hind coxa with punctation only on dorsal surface, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.31, 0.23), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus as equal in length as fourth tarsomere (0.15, 0.14). Wings (Fig. 223K, L). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction forming a slight stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; vein 2CUa absent and vein 2CUb spectral; vein 2 cu-a absent; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma (Fig. 223A, G, H, J). Metasoma laterally compressed. Petiole on T1 completely smooth and polished, with faint, satin-like sheen, parallel-sided in proximal half and then narrowing (length 0.42, maximum width 0.18, minimum width 0.08), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.16, length T2 0.16), edges of median area polished and lateral grooves deep, median area broader than long (length 0.16, maximum width 0.20, minimum width 0.08); T2 with pubescence in distal half. T3 longer than T2 (0.24, 0.16) and with pubescence more notorious in distal half. Pubescence on hypopygium dense. Cocoons (Fig. 223E). Gray or black oval cocoon with ordered silk fibers, but covered by a net. Comments. The coloration of the hind coxae is completely yellow (Fig. 223A, J), the body is stout, but elongated and covered by dense pubescence that may impede to see the sculpture on the body (Fig. 223A), and the limit between the mesopleuron and the metasternum is flattened. Male. Unknown. Etymology. Yanayacu Biological Station and Center for Creative Studies is an area of 100 hectares in the cloud forest of Ecuador, and the core site for much of our inventory work in the eastern Andes. Distribution. Parasitized caterpillar was collected in Ecuador, Napo, Yanayacu Biological Station (Stream trail), during August 2009 at 2,114 m in cloud forest. Biology. The lifestyle of this parasitoid species is solitary. Host. Undetermined species of Noctuidae feeding on Diplazium costale var. robustum (Dryopteridaceae). Caterpillar was collected in third instar., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles iangauldi Arias-Penna, sp. nov.Figs 105, 106 Female. Body length 2.32 mm, antenna length 2.63 mm, fore wing length 2.42 mm. Type material. Holotype: COSTA RICA • 1♀; 06-SRNP-8750, DHJPAR0012681; Área de Conservación Guanacaste, Alajuela, Sector San Cristóbal, Puente Palma; rain forest; 460 m; 10.9163, -85.37869; 24.x.2006; Anabelle Córdoba leg.; caterpillar collected in fourth instar; cocoons adhered to the larval cuticle and formed on 02.xi.2006; adult parasitoids emerged on 07.xi.2006; (CNC). Paratypes. • 9 (4♀, 5♂) (0♀, 0♂); 06-SRNP-8750, DHJPAR0012681; same data as for holotype; (CNC). Other material. Reared material. COSTA RICA: Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Sendero Corredor: • 51 (5♀, 4♂) (42♀, 0♂); 00-SRNP-11404, DHJPAR0000001; rain forest; 620 m; 10.87868, -85.38963; 08.vi.2000; Carolina Cano leg.; caterpillar collected in third instar; white separate cocoons packed in among the larval setae; adult parasitoids emerged on 28.vi.2000. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Río Areno : • 88 (3♀, 3♂) (81♀, 1♂); 05-SRNP-456, DHJPAR0004241; rain forest; 460 m; 10.91407, -85.38174; 04.ii.2005; Yessenia Mendoza leg.; caterpillar collected in fourth instar; masses of brownish small separate cocoons adhered to back of caterpillar; adult parasitoids emerged on 26.ii.2005. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Sendero Huerta: • 25 (3♀, 1♂) (21♀, 0♂); 06-SRNP-3984, DHJPAR0012016; rain forest; 527 m; 10.9305, -85.37223; 19.v.2006; Osvaldo Espinoza leg.; caterpillar collected in fifth instar; mass of vertical cocoons among the setae on the back of the caterpillar; adult parasitoids emerged on 11.vi.2006. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Vado Río Cucaracho : • 198 (5♀, 5♂) (132♀, 54♂); 01-SRNP-971, DHJPAR0000022; rain forest; 640 m; 10.8702, -85.39153; 20.iii.2001; Carolina Cano leg.; caterpillar collected in fourth instar; cocoons densely packed among the setae of the caterpillar; adult parasitoids emerged on 01.iv.2001and caterpillar still alive. • 93 (4♀ + 3♂) (76♀, 10♂); 04-SRNP-261, DHJPAR0000288; same data as for preceding except: 12.i.2004; Neyvin Hernandez; scattered cocoons adhered to cadaver; cocoon characteristics not reported; adult parasitoids emerged on 02.ii.2004. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Río Blanco Abajo : • 164 (3♀, 3♂) (152♀, 6♂); 02-SRNP-714, DHJPAR0001480; rain forest; 500 m; 10.90037, -85.37254; 05.ii.2002; Tom Prescott leg.; caterpillar collected in fifth instar; cocoons adhered to the larval cuticle; adult parasitoids emerged on 16.ii.2002. • 130 (3♀, 3♂) (110♀, 14♂); 02-SRNP-884, DHJPAR0000264; same data as for preceding except: 08.ii.2002; caterpillar collected in fourth instar; brown single cocoons adhered among the seta on larva; adult parasitoids emerged on 20.ii.2002. • 32 (3♀, 3♂) (19♀, 7♂); 07-SRNP-5154, DHJPAR0020729; same data as for preceding except: 24.xii.2007; Elda Araya; caterpillar collected in fourth instar; cocoons brown cocoons adhered among the setae of caterpillar; adult parasitoids emerged on 11.i.2008. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Puente Palma: • 133 (5♀, 5♂) (107♀, 16♂); 06-SRNP-9670, DHJPAR0012674; rain forest; 460 m; 10.9163, -85.37869; 28.xi.2006; Elda Araya leg.; caterpillar collected in third instar; solitary cocoons among the setae in the back of caterpillar cadaver densely packed upright; adult parasitoids emerged on 24.xii.2006. • 114 (5♀, 5♂) (81♀, 23♂); 06-SRNP-9671, DHJPAR0012670; same data as for preceding except: caterpillar collected in fourth instar; cocoons adhered to the larval cuticle; adult parasitoids emerged on 16.xii.2006. • 125 (5♀, 5♂) (99♀, 16♂); 09-SRNP-19, DHJPAR0034257; same data as for preceding except: 03.i.2009; caterpillar collected in fifth instar; cocoons adhered to the larval cuticle; cocoon characteristics not reported; adult parasitoids emerged on 09.i.2009. Área de Conservación Guanacaste , Alajuela, Sector Brasilia, Moga: • 43 (4♀, 4♂) (35♀, 0♂); 11-SRNP-65131, DHJPAR0042961; rain forest; 320 m; 11.01227, -85.34929; 29.iii.2011; Minor Carmona leg.; caterpillar collected in fifth instar; cocoons adhered to the larval cuticle and formed on 07.iv.2011; adult parasitoids emerged on 11.iv.2011. Diagnosis. Medioanterior pit of metanotum without median longitudinal carina (Figs 105C, 106E), propodeum medially rhomboid-shaped with transverse rugae (Figs 105C, 106E), scutellum in profile convex and slightly higher than mesoscutum (Figs 105E, 106G), propodeal spiracle without distal carina (Figs 105C, 106E), phragma of the scutellum partially exposed (Figs 105C, 106E), nucha surrounded by long radiating carinae (Figs 105C, 106E), propodeum medially rhomboid-shaped with transverse rugae (Figs 105C, 106E), dorsal carina delimiting a dorsal furrow on propleuron present (Fig. 105E), petiole on T1 parallel-sided, but narrowing over distal 1/3 (Figs 105D, 106F), precoxal groove deep (Figs 105A, 106A, G), anteroventral contour of mesopleuron straight/angulate or nearly so (Figs 105A, 106G), edges of median area on T2 polished and followed by a deep groove (Figs 105D, 106F), and fore wing with r vein curved, outer side of junction of r and 2RS veins forming a distinct stub (Figs 105I, 106J). Coloration (Fig. 105A). General body coloration brown-black except labrum, mandible, scape and pedicel yellow-brown; glossa, maxillary and labial palps, and tegulae yellow. Eyes and median ocellus silver, and lateral ocelli reddish (in preserved specimen). Fore and middle legs yellow except coxae (inner side lighter than outer side) and claws brown; hind legs yellow except brown-black coxae, most of the femora brown, distal half of tibiae brown, and tarsomeres brown. Petiole on T1 dark yellow-brown, contours darkened and sublateral areas yellow; T2 with median and adjacent areas brown, and lateral ends yellow; T3 broadly brown except proximal corners yellow; T4 and beyond completely brown; distally each tergum with a narrow whitish transparent band. In lateral view, T1-2 completely yellow; T3 yellow, distally with a narrow brown band; T4 and beyond completely brown. S1-3 yellow, but medially brown; S4 yellow, but distally with a broad brown band; penultimate sternum and hypopygium completely brown. Description. Head (Fig. 105A, B, E). Head rounded with pubescence short and dense. Proximal three antennal flagellomeres longer than wide (0.19:0.07, 0.19:0.07, 0.19:0.07), distal antennal flagellomere longer than penultimate (0.11:0.05, 0.09:0.05), antenna longer than body (2.63, 2.32); antennal scrobes-frons shallow. Face with dense fine punctations, interspaces with microsculpture, distal half dented only laterally and longitudinal median carina present. Frons smooth. Temple wide, punctate and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.09, 0.12). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally rounded and dorsally wide. Mesosoma (Fig. 105 A–C, E). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct proximally with polished area distally, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS, scutellar punctation scattered throughout, in profile scutellum convex and slightly higher than mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM; ATS demilune with short stubs delineating the area; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and homogeneous foveae, area just behind transscutal articulation smooth, shiny and nearly at the same level as mesoscutum (flat). Metanotum with BM wider than PFM (clearly differentiated); MPM circular without median longitudinal carina; AFM with a small lobe and not as well delineated as PFM; PFM thick and smooth; ATM proximally with semircular/undulate carina and distally smooth. Propodeum medially rhomboid-shaped with rugae, proximal half curved with fine sculpture and distal half relatively polished; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with a mix of rugae and fine punctation, dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron straight/angulate or nearly so; precoxal groove deep with faintly transverse lineate sculpture; epicnemial ridge convex, teardrop-shaped. Legs. Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.10, 0.07). Hind coxa with punctation only on ventral surface and dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.26, 0.19), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus shorter than fourth tarsomere (0.10, 0.13). Wings (Fig. 105I, J). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction forming a slight stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with a small smooth area; vein 2CUa absent and vein 2CUb spectral; vein 2 cu-a absent; vein 2-1A proximally tubular, distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe, subdistally and subproximally straightened, and setae present only proximally. Metasoma (Fig. 105A, D, F–H). Metasoma laterally compressed. Petiole on T1 finely sculptured only distally, virtually parallel-sided over most of length, but narrowing over distal 1/3 (length 0.33, maximum width 0.16, minimum width 0.08), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 distally losing definition (length median area 0.11, length T2 0.15), edges of median area polished and lateral grooves deep, median area broader than long (length 0.11, maximum width 0.16, minimum width 0.08); T2 with scattered pubescence only distally. T3 longer than T2 (0.20, 0.15) and with scattered pubescence throughout. Pubescence on hypopygium scattered. Cocoons (Fig. 4T). White or brown oval cocoons with silk fibers evenly smooth. Masses of separate cocoons adhered among the setae of caterpillar. Male (Fig. 106 A–K). Similar in coloration to female. The mesosoma is slightly stouter than female. Etymology. Ian David Gauld (25 May 1947-12 January 2009) is a well-known British entomologist who dedicated his entire career to the evolutionary biology of Ichneumonids (Anomaloniae, Labeninae, Ophioninae, and Pimplinae). He spent the last two decades of his life focusing upon the Costa Rican fauna. Distribution. Parasitized caterpillars were collected in Costa Rica, ACG, Sector Brasilia (Moga) and Sector San Cristóbal (Río Areno, Río Blanco Abajo, Puente Palma, Sendero Corredor, Sendero Huerta, and Vado Río Cucaracho), during June 2000, March 2001, February 2002 and 2005, January 2004, May, October-November 2006, December 2007, January 2009, and March 2011 at 460 m, 500 m, 620 m, and 640 m in rain forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Zanola verago Cramer (Apatelodidae) (Fig. 4T) feeding on Iresine diffusa (Amaranthaceae), Philodendron sp. (Araceae), Psychotria berteriana, Hamelia patens and Spermacoce ocymifolia (Rubiaceae), Inga oerstediana and I. samanensis (Fabaceae), Solanum circinatum (Solanaceae). Caterpillars were collected in third, fourth, and fifth instar., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles annettewalkerae Arias-Penna, sp. nov.Figs 23, 24 Female. Body length 3.18 mm, antenna length 3.28 mm, fore wing length 3.13 mm. Type material. Holotype: COSTA RICA • 1♀; 10-SRNP-35889, DHJPAR0041868; Área de Conservación Guanacaste, Guanacaste, Sector Cacao, Sendero Nayo; cloud forest; 1,090 m; 10.92446, -85.46953; 24.viii.2010; Dunia Garcia leg.; caterpillar collected in third instar; cocoon formed on 01.ix.2010; adult parasitoids emerged on 09.ix.2010; (CNC). Paratypes. • 1 (0♀, 0♂) (0♀, 1♂); 10-SRNP-35885, DHJPAR0041861; same data as for holotype except: single white cocoon formed on 01.ix.2010; adult parasitoids emerged on 08.ix.2010; (CNC). • 1 (0♀, 1♂) (0♀, 0♂); 10-SRNP-35883, DHJPAR0041865; same data as for holotype except: single white cocoon (not white bud-like cocoon) adhered to the leaf substrate; adult parasitoids emerged on 10.ix.2010; (CNC). • 1 (0♀, 0♂) (0♀, 1♂); 10-SRNP-35888, DHJPAR0041866; same data as for holotype except: adult parasitoids emerged on 10.ix.2010; (CNC). Other material. Reared material. COSTA RICA: Área de Conservación Guanacaste , Alajuela, Sector Rincón Rain Forest , Sendero Albergue Crater: • 1 (0♀, 0) (1♀, 0♂); 10-SRNP-1390, DHJPAR0039020; 980 m; 10.84886, -85.3281; 14.iii.2010; Carolina Cano leg.; caterpillar collected in third instar; cocoon adhered to the leaf substrate and formed on 16.iii.2010; adult parasitoids emerged on 26.iii.2010. Diagnosis. Propleuron with fine punctations throughout (Fig. 23C, J), axillary trough of metanotum with undulate carinae throughout (Figs 23G, 24C), medioposterior band of scutellum not overlapping the medioanterior pit of metanotum (Figs 23F, 24C), longitudinal median carina on face absent (Fig. 23B), inner margin of eyes diverging slightly at antennal sockets (Fig. 23B), scutellar punctation distinct throughout (Figs 23F, 24B), fore wing with tubular vein 1 cu-a incomplete/broken, not reaching the edge of 1-1A vein, r vein curved, outer side of junction of r and 2RS veins forming a slight stub (Fig. 23L), petiole on T1 evenly narrowing over its length (Figs 23H, 24D), surface of metasternum flat or nearly so, edges of median area on T2 obscured by weak longitudinal stripes (Figs 23H, I, 24D, G), and dorsal outer depression on hind coxa absent (Fig. 24A, F). Coloration (Fig. 23 A–M). General body coloration black except apex of pedicel and clypeus yellow-brown; labrum, mandibles, and tegulae yellow; glossa, maxillary and labial palps ivory; distal 1/3 of propleuron and some spots on dorsal and ventral edge of pronotum yellow-brown. Eyes purple (in preserved specimen) and ocelli silver. Fore and middle legs light yellow or ivory although edges from femora to tarsomeres yellow, and claws brown; hind legs with trochanter and trochantellus ivory, coxae proximally black with apex ivory, femora ivory and with a tiny distal brown spot, tibiae yellow with both ends brown, and tarsomeres brown. Petiole on T1 black and sublateral areas pale yellow; T2 with median and adjacent areas black, and narrow lateral ends yellow; T3 with a medial brown area that coincides with the width of median and adjacent areas on T2; T4 and beyond brown; distally each tergum with a narrow yellowish transparent band. In lateral view, T1-3 completely light yellow or ivory; T4 and beyond yellow, but dorsally brown, extent of brown area increasing from proximal to distal. All sterna light yellow or ivory, although hypopygium medially yellow-brown; ovipositor sheaths brown. Description. Head (Fig. 23 A–D). Head triangular with long and dense pubescence. Proximal three antennal flagellomeres longer than wide (0.29:0.08, 0.28:0.08, 0.26:0.08); antenna longer than body (3.28, 3.18); antennal scrobes-frons shallow. Face convex with scattered and finely punctate, interspaces smooth, and longitudinal median carina absent. Frons smooth. Temple wide, punctate and interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.14). Malar suture present. Median area between lateral ocelli without depression. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma (Fig. 23A, E–G, J). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct throughout, interspaces smooth. Scutellum triangular, apex sloped and fused with BS, in profile scutellum flat and on same plane as mesoscutum, scutellar punctation distinct throughout, phragma of the scutellum partially exposed; BS not overlapping the MPM; ATS demilune with a little, complete parallel carinae; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and homogeneous foveae; area just behind transscutal articulation with same kind of sculpture as mesoscutum and with a sloped transverse strip. Metanotum with BM wider than PFM (clearly differentiated); MPM circular with some sculpture inside; AFM without setiferous lobes and not as well delineated as PFM; PFM thick and smooth; ATM with undulate carinae throughout. Propodeum without median longitudinal carina, proximal half curved and with rather coarse sculpture and distal half rugose with a shallow dent at each side of nucha; distal edge of propodeum with a flange at each side and short stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum and dorsal furrow smooth, but ventral furrow with short parallel carinae. Propleuron with fine punctations throughout and dorsally without a carina. Metasternum flat or nearly so. Contour of mesopleuron convex; precoxal groove smooth, shiny and shallow, but visible; epicnemial ridge convex, teardrop-shaped. Legs. Ventral margin of fore telotarsus entire without seta; fore telotarsus proximally narrow and distally wide and longer than fourth tarsomere (0.14, 0.07). Hind coxa finely punctate throughout, and dorsal outer depression absent. Inner spur of hind tibia longer than outer spur (0.30, 0.25), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.20, 0.15). Wings (Fig. 23L, M). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction forming a slight stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A present only proximally as spectral vein; tubular vein 1 cu-a straight and incomplete/broken, not reaching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally evenly convex, subproximally evenly convex, and setae evenly scattered in the margin. Metasoma (Fig. 23A, H, I, K). Metasoma cylindrical. Petiole on T1 with rugae all over except antero-median depression, petiole evenly narrowing distally (length 0.45, maximum width 0.20, minimum width 0.14) with apex truncate, and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.21, length T2 0.21); edges of median area obscured by weak longitudinal stripes, median area broader than long (length 0.21, maximum width 0.40, minimum width 0.10); T2 with scattered pubescence only distally. T3 longer than T2 (0.26, 0.21), T3 with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoon. White cocoon with silk fibers messy/disordered/fluffy. Single cocoon adhered to the leaf substrate. Comments. The antenna is broken, only with 11 flagellomeres. Male (Fig. 24 A–G). The body coloration is darker than in female: light yellow-brown rather than light yellow/ivory. The sterna are completely yellow-brown with arthrodial membranes yellow; the hind coxa is black with apex yellow. The lateral margins of the median area on T2 are slightly curved (convex, Fig. 24D, G). Etymology. Named after Annette K. Walker, now in New Zealand but previously in London specializing in Microgastrinae as part of the Commonwealth Institute of Entomology. Distribution. Parasitized caterpillars were collected in Costa Rica, ACG, Sector Cacao (Sendero Nayo) and Sector Rincón Rain Forest (Sendero Albergue Crater), during March and August 2010 at 980 m and 1,090 m in cloud forests. Biology. The lifestyle of this parasitoid species is solitary. Host. Syllepte nitidalis Dognin (Crambidae: Spilomelinae) feeding on Malvaviscus arboreus (Malvaceae) and Trichaea pilicornis Herrich-Schäffer (Crambidae: Spilomelinae) feeding on Psychotria panamensis (Rubiaceae). Caterpillars were collected in third instar., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles meganmiltonae Arias-Penna, sp. nov.Figs 158, 159 Female. Body length 2.17 mm, antenna length 2.37 mm, fore wing length 2.58 mm. Type material. Holotype: COSTA RICA • 1♀; 06-SRNP-9041, DHJPAR0012683; Área de Conservación Guanacaste, Alajuela, Sector San Cristóbal, Finca San Gabriel; rain forest; 645 m; 10.87766, -85.39343; 05.xi.2006; Elda Araya leg.; caterpillar collected in third instar; cocoons adhered to the leaf substrate; adult parasitoids emerged on 16.xi.2006; (CNC). Paratypes. • 21 (4♀, 4♂) (0♀, 13♂); 06-SRNP-9041, DHJPAR0012683; same data as for holotype; (CNC). Other material. Reared material. COSTA RICA: Área de Conservación Guanacaste , Guanacaste, Sector Del Oro, Quebrada Raíz: • 1 (1♀, 0♂) (0♂, 0♀); 04-SRNP-55920, DHJPAR0004238; dry-rain intergrade forest; 280 m; 11.02865, -85.48669; 15.xi.2004; Lucia Ríos leg.; caterpillar collected in fourth instar; elongate ridged white bud-like cocoons adhered to the leaf substrate and formed on 20.xi.2004; adult parasitoids emerged on 27.xi.2004. Área de Conservación Guanacaste , Alajuela, Sector San Cristóbal, Finca San Gabriel: • 16 (5♀, 2♂) (9♀, 0♂); 09-SRNP-6146, DHJPAR0038065; rain forest; 645 m; 10.87766, -85.39343; 20.xi.2009; Elda Araya leg.; caterpillar collected in fifth instar; medium fluffy white cocoons adhered together and adhered to the leaf substrate; adult parasitoids emerged on 01.xii.20069. Área de Conservación Guanacaste , Guanacaste, Sector Mundo Nuevo, Vado Miramonte: • 16 (3♀, 1♂) (12♀, 0♂); 10-SRNP-57267, DHJPAR0041715; dry-rain intergrade forest; 305 m; 10.77175, -85.43400; 14.xi.2010; José Cortéz leg.; caterpillar collected in fifth instar; cocoons formed on 19.xi.2010; adult parasitoids emerged on 30.xi.2010. • 12 (0♀, 3♂) (0♀, 9♂); 10-SRNP-57355, DHJPAR0041650; same data as for preceding except: 15.xii.2010; caterpillar collected in fourth instar; cocoons formed on 20.xii.2010; adult parasitoid emerged on 28.xii.2010. Área de Conservación Guanacaste , Alajuela, Sector Rincón Rain Forest , Camino Albergue Oscar: • 5 (2♀, 1♂) (2♀, 0♂); 10-SRNP-6685, DHJPAR0041625; 560 m; 10.87741, -85.32363; 10.xi.2010; Carolina Cano leg.; caterpillar collected in fifth instar; cocoons adhered to the leaf substrate and formed on 16.xi.2010; adult parasitoids emerged on 23.xi.2010. • 11 (1♀, 3♂) (0♀, 4♂); 10-SRNP-6687, DHJPAR0041834; same data as for preceding except: multiple white cocoons adhered in host cocoon; date of cocoons not reported; adult parasitoid emerged on 22.xi.2010. • 11 (3♀, 3♂) (3♀, 2♂); 10-SRNP-6688, DHJPAR0041620; same data as for preceding except: multiple white cocoons adhered in host cocoon; date of cocoons not reported. • 12 (3♀, 1♂) (8♀, 0♂); 10-SRNP-6689, DHJPAR0041839; same data as for preceding except: adult parasitoids emerged on 26.xi.2010. Área de Conservación Guanacaste , Alajuela, Sector Rincón Rain Forest , San Lucas: • 1 (0♀, 0♂) (0♀, 1♂); 11-SRNP-41426, DHJPAR0043167; 320 m; 10.91847, -85.30338; 24.iii.2011; Anabelle Córdoba leg.; caterpillar collected in fourth instar; cocoons adhered to the leaf substrate; adult parasitoids emerged on 08.iv.2011. • 8 (2♀, 1♂) (5♀, 0♂); 11-SRNP-41427, DHJPAR0042884; same data as for preceding. Diagnosis. Fore telotarsus basally narrow, apically wide (Fig. 158A), ventral margin with a tiny curved seta, medioposterior band of scutellum not overlapping the medioanterior pit of metanotum (Fig. 159B, C), phragma of the scutellum widely visible (Figs 158B, 159C), fore wing with vein 2 cu-a absent, r vein straight, outer side of junction of r and 2RS veins forming a stub (Figs 158G, 159H), median area on T2 broader than long (Fig. 159D), edges of median area on T2 obscured by weak longitudinal stripes (Figs 158C, 159D), and lateral grooves delimiting the median area on T2 distally losing definition on T2, vertex in dorsal view wide (Fig. 159E), in lateral view, metasoma laterally compressed (Figs 158F, 159F), T3 longer than T2 (Fig. 159G), inner margin of eyes diverging slightly at antennal sockets, petiole on T1 evenly narrowing distally (wide base to a narrow apex) and finely sculptured (Fig. 159D, G), and propodeum without a median longitudinal dent (Fig. 159C). Coloration (Fig. 158A). General body coloration dark brown except scape and pedicel yellow-brown with inner sides brown; first four-five proximal antennal flagellomeres dorsally lighter (light brown) than ventrally (dark brown), remaining flagellomeres dark brown on both sides; clypeus and labrum light brown; mandibles and tegulae dark yellow; glossa, maxillary and labial palps yellow; propleuron and both dorsal and ventral furrows of pronotum lighter than mesosoma coloration. Eyes and ocelli reddish (in preserved specimen). Fore and middle legs yellow except claws brown; hind legs yellow except coxae proximally with a edge light brown forming a irregular shape, a tiny brown spot in femora, tibiae distally light brown, and tarsomeres yellow-brown. Petiole on T1 with two colorations, proximal 1/3 reddish/yellow-brown and distal 2/3 brown, contours darkened and sublateral areas yellow; T2 with median and adjacent areas brown, and lateral ends yellow; T3 almost completely brown, but with a tiny yellow area in proximal corner; T4 and beyond completely brown; distally each tergum with a narrow yellowish transparent band. In lateral view, T1-2 completely yellow; T3 yellow with a small dorsodistal brown area; T4 and beyond brown. S1-4 yellow; penultimate sternum and hypopygium light brown/brown. Description. Head (Fig. 158A, E). Head rhomboid with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.22:0.07, 0.18:0.07, 0.18:0.07), distal antennal flagellomere longer than penultimate (0.11:0.07, 0.08:0.07), antenna longer than body (2.37, 2.17); antennal scrobes-frons sloped and forming a shelf. Face flat or nearly so, with dense fine punctations, interspaces with microsculpture and longitudinal median carina present. Frons smooth. Temple wide, punctate and interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.12). Malar suture present. Median area between lateral ocelli without depression. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma (Fig. 158A, B, E). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct throughout, interspaces smooth. Scutellum long and slender, apex sloped and fused with BS, scutellar punctation indistinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum widely visible; BS not overlapping the MPM; ATS demilune inner side with a row of foveae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation wih large and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM wider than PFM (clearly differentiated); MPM proximally circle and distally straight with a short distal carina; AFM with a small lobe and not as well delineated as PFM; PFM slim and smooth; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum finely sculptured, without median longitudinal carina, proximal half curved; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum and both dorsal and ventral furrows smooth. Propleuron with fine punctations throughout and dorsally without a carina. Metasternum flat or nearly so. Contour of mesopleuron convex; precoxal groove smooth, shiny and shallow, but visible; epicnemial ridge elongated more fusiform (tapering at both ends). Legs. Ventral margin of fore telotarsus entire, but with a tiny curved seta, fore telotarsus proximally narrow and distally wide, and longer than fourth tarsomere (0.10, 0.05). Hind coxa with dorsal half sparsely punctate, ventral half densely punctate, and dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.21, 0.16), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus as equal in length as fourth tarsomere (0.12, 0.11). Wings (Fig. 158G, H). Fore wing with r vein straight; 2RS vein straight; r and 2RS veins forming an angle at their junction and outer side of junction forming a slight stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae almost homogeneously distributed as the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a absent; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a straight, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma (Fig. 158A, C, D, F). Metasoma laterally compressed. Petiole on T1 finely sculptured throughout, evenly narrowing distally (length 0.30, maximum width 0.15, minimum width 0.09) and with scattered pubescence on distal half. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.15, length T2 0.15), edges of median area obscured by weak longitudinal stripes, median area broader than long (length 0.15, maximum width 0.23, minimum width 0.07); T2 with scattered pubescence only distally. T3 longer than T2 (0.20, 0.15) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. White bud-like cocoons with medium fluffy silk fibers. Cocoons adhered to the leaf substrate or in host cocoon Comments. The antennal scrobes (frons) form a shelf-shaped, so this area is strongly sloped; the scape is very swollen with inner sides curved. Some specimens body with lighter pale brown coloration, although the color pattern can de distinguished, maybe they emerged early. In other females, the epicnemial ridge is lighter than mesosoma coloration. The penultimate sternum and the hypopygium are yellow-brown. Some females (e.g., 04-SRNP-55920) exhibit coxae completely dark brown and the hypopygium brown, but medially yellow-brown. Male (Fig. 159 A–I). In some specimens (e.g., 10-SRNP-57355), the mesoscutum is reddish/dark yellow-brown, coloration taking the place of notauli; the coloration on these specimens is more reddish than yellow; in some specimens the hind coxa is completely light brown. Etymology. Megan Milton currently is a data and communication lead at Barcode of Life Data Systems (BOLD). Distribution. The parasitized caterpillars were collected in Costa Rica, ACG, Sector Mundo Nuevo (Vado Miramonte), Sector Rincón Rain Forest (Camino Albergue Oscar and San Lucas), Sector San Cristóbal (Finca San Gabriel), and Sector Del Oro (Quebrada Raíz), during November 2004, 2006, and 2009; November-December 2010; and March 2011 at 280 m, 305 m, 320 m, 560 m, and 645 m in dry-rain intergrade forest and rain forest. Biology. The lifestyle of this parasitoid species is solitary/gregarious. Host. Herpetogramma sp. Lederer (Crambidae: Spilomelinae) feeding on Achyranthes aspera, A. indica and Alternanthera pubiflora (Amaranthaceae). Caterpillars were collected in third, fourth and fifth instar., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles shelbystedenfeldae Arias-Penna, sp. nov.Figs 201, 202 Male. Body length 2.17 mm, antenna length 3.08 mm, fore wing length 3.68 mm. Type material. Holotype: COSTA RICA • 1♀; 00-SRNP-24000, DHJPAR0013608; Área de Conservación Guanacaste, Guanacaste, Sector Santa Rosa, Bosque Humedo; dry forest; Malasie; 290 m; 10.85145, -85.60801; 24.iv.2000; DH Janzen & W Hallwachs leg.; (CNC). Paratypes. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-23957, DHJPAR0013596; same data as for holotype except: 10.iv.2000; (CNC). Other material. Malaise-trapped material. COSTA RICA: Área de Conservación Guanacaste , Guanacaste, Sector Santa Rosa, Bosque Humedo: • 1 (1♀, 0♂) (0♀, 0♂); 98-SRNP-16106, DHJPAR0013373; Malaise; dry forest; 290 m; 10.85145, -85.60801; 09.iii.1998; DH Janzen & W Hallwachs leg. • 1 (0♀, 1♂) (0♀, 0♂); 98-SRNP-16130, DHJPAR0013579; same data as for preceding except: 30.iii.1998. • 1 (0♀, 0♂) (0♀, 1♂); 99-SRNP-19079, DHJPAR0013581; same data as for preceding except: 03.v.1999. • 1 (0♀, 1♂) (0♀, 0♂); 99-SRNP-19086, DHJPAR0013583; same data as for preceding except: 03.v.1999. • 1 (0♀, 1♂) (0♀, 0♂); 99-SRNP-19096, DHJPAR0013587; same data as for preceding except: 10.v.1999. • 1 (0♀, 1♂) (0♀, 0♂); 00-SRNP-24025, DHJPAR0013408; same data as for preceding except: 06.iii.2000. • 1 (0♀, 0♂) (0♀, 1♂); 00-SRNP-24006, DHJPAR0013414; same data as for preceding except: 27.iii.2000. • 1 (0♀, 1♂) (0♀, 0♂); 00-SRNP-23961, DHJPAR0013599; same data as for preceding except: 22.v.2000. • 1 (0♀, 1♂) (0♀, 1♂); 00-SRNP-23988, DHJPAR0013607; same data as for preceding except: 22.v.2000. • 1 (0♀, 1♂) (0♀, 0♂); 07-SRNP-15057, DHJPAR0013617; same data as for preceding except: 05.vi.2007; AR Deans & J Rodriguez leg. Área de Conservación Guanacaste , Guanacaste, Sector Santa Rosa, Bosque San Emilio: • 1 (0♀, 1♂) (0♀, 0♂); 00-SRNP-23869, DHJPAR0013555; Malaise; dry forest; 300 m; 10.84389; -85.61384; 22.v.2000; DH Janzen & W Hallwachs leg. Diagnosis. Precoxal groove with transverse lineate sculpture (Figs 201A, I, 202E), medioanterior pit of metanotum circular and bisected by a median longitudinal carina (Figs 201F, 202C), inner margin of eyes diverging slightly at antennal sockets (Fig. 201B), scutellar punctation distinct throughout (Figs 201E, F, 202B, C), fore wing with 1 cu-a vein complete, but junction with 1-1A vein spectral, outer side of junction of r and 2RS veins not forming a stub (Fig. 202D), propodeum with a clearly visible median longitudinal carina (Figs 201F, 202C), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (Figs 201G, H). Coloration (Fig. 202 A–F). General body coloration black except scape yellow-brown/reddish distally with a brown band; pedicel yellow-brown/reddish; first four-five proximal antennal flagellomeres dorsally lighter (light brown) than ventrally (dark brown), remaining flagellomeres dark brown on both sides; labrum, mandible, and tegulae yellow; propleuron distally with a tiny dot, dorsal furrow of pronotum, and epicnemial ridge with yellow-brown/reddish tints; glossa, maxillary and labial palps pale yellow/ivory. Eyes and ocelli silver. Fore and middle legs dark yellow except brown claws; hind legs dark yellow except black coxae, femora distally brown, and tibiae with both ends brown. Petiole on T1 light brown, contours darkened and sublateral areas ivory/pale yellow; T2 with median and adjacent areas brown, and lateral ends ivory/pale yellow; T3 brown, but 1/3 proximal of lateral ends ivory/pale yellow; T4 and beyond completely brown; distally each tergum with a narrow yellowish translucent band. In lateral view, T1-3 ivory/pale yellow; T4 and beyond yellow with brown tints. S1-2 ivory/pale yellow; S3 yellow, but medially brown; S4 and beyond brown. Description. Head (Fig. 202A). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.19:0.07, 0.20:0.07, 0.19:0.07), distal antennal flagellomere longer than penultimate (0.13:0.05, 0.10:0.05), antenna longer than body (3.08, 2.17); antennal scrobes-frons shallow. Face convex with dense fine punctations, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL subequal in length with OOL (0.09, 0.10). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma (Fig. 202 A–C, E). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct throughout, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS, scutellar punctation distinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS not overlapping the MPM; ATS demilune with a little, complete and parallel carinae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM wider than PFM (clearly differentiated); MPM circular and bisected by a median longitudinal carina; AFM with a small lobe and not as well delineated as PFM; PFM thick, smooth and with lateral ends rounded; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum with a clearly visible median longitudinal carina, proximal half curved rather coarse sculpture and distal half rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; both dorsal and ventral furrows and central area of pronotum with sculpture. Propleuron rugose and dorsally with a carina. Metasternum convex. Contour of mesopleuron convex; precoxal groove deep with transverse lineate sculpture; epicnemial ridge convex, teardrop-shaped. Legs (Fig. 202A, F). Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.10, 0.06). Medially hind coxa smooth, dorsally with scattered punctation and ventrally with dense punctation, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.23, 0.17), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus as equal in length as fourth tarsomere (0.11, 0.11). Wings (Fig. 202D). Fore wing with r vein curved; 2RS vein slightly concave; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with a small smooth area; vein 2CUa absent and vein 2CUb spectral; vein 2 cu-a absent; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved and complete, but junction with 1-1A vein spectral. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma (Fig. 202A, F). Metasoma laterally compressed. Petiole on T1 finely sculptured only distally, evenly narrowing distally (length 0.31, maximum width 0.15, minimum width 0.04) and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.11, length T2 0.11), edges of median area polished and lateral grooves deep, median area broader than long (length 0.11, maximum width 0.14, minimum width 0.04); T2 with scattered pubescence only distally. T3 longer than T2 (0.19, 0.11) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. Unknown. Comments. The sculpture on body are dense (Fig. 201A, F), the hind coxae is stout, and both sexes with stout and short bodies. Female (Fig. 201 A–J). The metasoma is missing in the only preserved female; however, there is a photographic record. In female, the distal corners of mesoscutum are yellow-brown/reddish. Etymology. Shelby E. Stedenfeld as graduate student at the Kentucky University, Lexington, KY, USA, was interested in the taxonomy and systematics of Ichneumonidae and their roles in biological control. Distribution. The adult parasitoids were collected in Costa Rica, ACG, Sector Santa Rosa (Bosque Humedo and Bosque San Emilio), during May 1999, March-May 2000, and June 2007 at 290 m in dry forest. Biology. Unknown. Host. Unknown., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786

Glyptapanteles daveschindeli Arias-Penna, sp. nov.Figs 64, 65 Female. Body length 2.02 mm, antenna length 2.22 mm, fore wing length 1.96 mm. Type material. Holotype: COSTA RICA • 1♀; 08-SRNP-16708, DHJPAR0030699; Área de Conservación Guanacaste, Guanacaste, Sector Santa Rosa, Área administrativa; dry forest; 295 m; 10.83764, -85.61871; 14.xi.2008; Lucia Vargas leg.; caterpillar collected in fifth instar; cocoons adhered to caterpillar body in rings; adult parasitoids emerged on 26.xi.2008; (CNC). Paratypes. • 28 (2♀, 2♂) (25♀, 0♂); 08-SRNP-16708, DHJPAR0030699; same data as for holotype; (CNC). Other material. Reared material. COSTA RICA: Área de Conservación Guanacaste , Guanacaste, Sector Santa Rosa, Bosque Humedo: • 49 (6♀, 1♂) (42♀, 0♂); 93-SRNP-2251, DHJPAR0000071; dry forest; 290 m; 10.85145, -85.60801; 14.vi.1993; gusaneros leg.; caterpillar collected in fifth instar; brown/gray cocoons adhered laterally against caterpillar body in rings; adult parasitoids emerged on 16.vi.1993. Área de Conservación Guanacaste , Guanacaste, Sector Cacao, Sendero Guayabal: • 8 (3♀, 0♂) (5♀, 0♂); 08-SRNP-45004, DHJPAR0020724; cloud forest; 500 m; 10.88571, -85.48184; 03.i.2008; Manuel Pereira leg.; caterpillar collected in third instar; brown cocoons adhered to the larval cuticle and formed on 24.i.2008; adult parasitoids emerged on 04.ii.2008. Malaise-trapped material. COSTA RICA: Área de Conservación Guanacaste , Guanacaste, Sector Santa Rosa, Bosque San Emilio: • 1 (1♀, 0♂) (0♀, 0♂); 99-SRNP-19008, DHJPAR0013385; dry forest; 300 m; 10.84389, -85.61384; Malaise trap; 10.v.1999; DH Janzen & W Hallwachs leg. Diagnosis. Mesoscutum punctation proximally distinct, but distally absent/dispersed (Figs 64B, 65B), phragma of the scutellum completely concealed (Figs 64C, 65C), antenna longer than body, ventral margin of fore telotarsus slightly excavated, scutellar punctation indistinct throughout (Figs 64B, 65B), propodeal spiracle without distal carina (Figs 64C, 65C), petiole on T1 distally with lateral margins relatively straight and finely sculptured only laterally (Figs 64D, G, 65D, G), surface of metasternum flat or nearly so, precoxal groove deep with lineate sculpture (Figs 64A, E, 65A, E), fore wing with vein 1 cu-a curved, r vein curved, outer side of junction of r and 2RS veins not forming a stub (Figs 64I, 65I), dorsal outer depression on hind coxa present (Figs 64A, F, 65A, F), inner margin of eyes diverging slightly at antennal sockets, propodeum without median longitudinal carina (Figs 64C, 65C), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (Figs 64D, G, 65D, G). Coloration (Fig. 64A). General body coloration brown-black although some areas on body are light brown/reddish as clypeus, propleuron, both dorsal and ventral furrows of pronotum, epicnemial ridge, ventral edge of mesopleuron, distal corner of mesoscutum, lunules, BS, and PFM; scape, pedicel, labrum and mandibles yellow-brown; tegulae yellow; first five-six proximal antennal flagellomeres dorsally lighter (light brown) than ventrally (dark brown), remaining flagellomeres dark brown on both sides; glossa, maxillary and labial palps, and tegulae yellow. Fore legs yellow except brown claws; middle legs yellow except coxae and claws brown; hind legs brown except trochanter, trochantellus, proximal half of tibiae, and tibial spurs yellow, basitarsus with a proximal yellow ring, last two distal tarsomeres lost. Petiole on T1 with two colorations: proximal half brown-orange and distal half brown, contours darkened, and sublateral areas yellow-brown; T2 with median area brown, adjacent area yellow-brown and very narrow although proximal wide, and lateral ends yellow-brown; T3 extended brown, lateral ends narrow and yellow-brown; T4 and beyond completely brown; distally each tergum with a narrow whitish transparent band. In lateral view, T1-3 completely yellow; T4 and beyond completely brown. S1-3 completely yellow; S4 proximal half yellow, distal half brown; penultimate sternum and hypopygium completely brown. Description. Head (Fig. 64A, B) Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.16:0.05, 0.16:0.0, 0.16:0.05), distal antennal flagellomeres longer than penultimate (0.10:0.05, 0.08:0.05), antenna longer than body (2.22, 2.02); antennal scrobes-frons shallow. Face with dense fine punctations, interspaces wavy, distal half dented only laterally, and longitudinal median carina present. Frons smooth. Temple narrow, punctate and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.09, 0.13). Malar suture present. Median area between lateral ocelli without depression. Vertex laterally rounded and dorsally wide. Mesosoma (Fig. 64 A–C, E). Mesosoma dorsoventrally convex. Distal 1/3 of mesoscutum with lateral margin slightly dented, punctation proximally distinct, but distally absent/dispersed, and interspaces with microsculpture. Scutellum triangular, apex sloped and fused with BS, scutellar punctation indistinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum completely concealed; BS only very partially overlapping the MPM; ATS demilune with short stubs delineating the area; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation with a smooth and shiny sloped transverse strip. Metanotum with BM wider than PFM (clearly differentiated); MPM circular without median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM; PFM thick and smooth; ATM proximally with semircular/undulate carina and distally smooth. Propodeum without median longitudinal carina, proximal half weakly curved with medium-sized sculpture and distal half with a shallow dent at each side of nucha or rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum virtually without trace of dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with fine punctations throughout and dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron straight/angulate or nearly so; precoxal groove deep with faintly transverse lineate sculpture; epicnemial ridge convex, teardrop-shaped. Legs. Ventral margin of fore telotarsus slightly excavated and with a tiny curved seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.10, 0.06). Hind coxa with punctation only on ventral surface and dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.20, 0.12), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Wings (Fig. 64I, J). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell proximal half smooth; vein 2CUa absent and vein 2CUb spectral; vein 2 cu-a absent; vein 2-1A present only proximally as tubular vein; tubular vein 1 cu-a curved and complete, but junction with 1-1A vein spectral. Hind wing with vannal lobe wide, subdistally and subproximally straightened, and setae present only proximally. Metasoma (Fig. 64A, D, F–H). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, virtually parallel-sided over most of length, but narrowing over distal 1/3 (length 0.27, maximum width 0.14, minimum width 0.06), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.12, length T2 0.12), edges of median area polished and lateral grooves deep, median area broader than long (length 0.12, maximum width 0.18, minimum width 0.06); T2 with a distinctive row of pubescence only at the distal margin. T3 longer than T2 (0.17, 0.12) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons (Fig. 4U). Brown or gray oval cocoon with evenly smooth silk fibers. Cocoons were adhered laterally against the caterpillar's body in rings. Comments. Some females from the same sample as holotype do not exhibit brown-orange petiole at the proximal part; instead the petiole is completely brown. The proximal half of propodeum with rugae at each side of the nucha. Male (Fig. 65 A–J). Similar in coloration to female, although the mesosoma is stouter and more robust than female. Etymology. David (Dave) Schindel is the executive secretary of the Consortium for the Barcode of Life (CBOL). He works at the National Museum of Natural History, Washington, DC, USA. Distribution. The parasitized caterpillars were collected in Costa Rica, ACG, Sector Cacao (Sendero Guayabal) and Sector Santa Rosa (Área administrative and Bosque Humedo), during June 1993 and January and November 2008 at 290 m, 295 m, and 500 m in dry forest and cloud forest. The adult parasitoids were Malaise-trapped in Costa Rica, ACG, Sector Santa Rosa (Bosque San Emilio), during May 1999 at 300 m in dry forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Oxydia apidania Cramer (Geometridae: Ennominae) feeding on Inga punctata (Fabaceae), O. vesulia (Cramer) (Fig. 4U) feeding on Spondias purpurea, introduced species, (Anacardiaceae), and Oxydia sp. although food plant was not reported. Caterpillars were collected in third and fifth instar., Published as part of Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, pp. 1-685 in ZooKeys 890 on page 1, DOI: 10.3897/zookeys.890.35786