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Let A be a unital simple separable locally approximately subhomogeneous C*-algebra (locally ASH algebra). It is shown that A ⊗ Q can be tracially approximated by unital Elliott–Thomsen algebras with trivial K 1 -group, where Q is the universal UHF algebra. In particular, it follows that A is classifiable by the Elliott invariant if A is Jiang–Su stable. [ABSTRACT FROM AUTHOR]

Abstract: Let A be an AH algebra, that is, A is the inductive limit -algebra of with , where are compact metric spaces, and are positive integers, and are projections. Suppose that A has the ideal property: each closed two-sided ideal of A is generated by the projections inside the ideal, as a closed two-sided ideal. Suppose that . (This condition can be relaxed to a certain condition called very slow dimension growth.) In this article, we prove that if we further assume that is torsion free, then A is an approximate circle algebra (or an algebra), that is, A can be written as the inductive limit of where . One of the main technical results of this article, called the decomposition theorem, is proved for the general case, i.e., without the assumption that is torsion free. This decomposition theorem will play an essential role in the proof of a general reduction theorem, where the condition that is torsion free is dropped, in the subsequent paper Gong et al. (preprint) —of course, in that case, in addition to space , we will also need the spaces , , and , as in Gong (2002) . [Copyright &y& Elsevier]

While achieving rapid developments in recent years, bispecific antibodies are still difficult to design and manufacture, due to mispair of both heavy and light chains. Here we report a novel technology to make bispecific molecules. The knob-into-hole method was used to pair two distinct heavy chains as a heterodimer. IgG4 S228P CH1-CL interface was then partially replaced by T-cell receptor α/β constant domain to increase the efficiency of cognate heavy and light chain pairing. Following expression and purification, the bispecific antibody interface exchange was confirmed by Western blotting and LC–MS/MS. To ensure its validity, we combined a monovalent bispecific antibody against PD-1 (sequence from Pembrolizumab) and LAG3 (sequence from Relatlimab). The results showed that the molecule could be assembled correctly at a ratio of 95% in cells. In vitro functional assay demonstrated that the purified bispecific antibody exhibits an enhanced agonist activity compared to that of the parental antibodies. Low immunogenicity was predicted by an open-access software and ADA test. [ABSTRACT FROM AUTHOR]

The red arrow indicates a Tm1 value of 62.45°C." now reads: "C, The Tm1 of protein was quantitatively detected by Uncle biologics stability screening platform. S5C, the melting temperature (Tm1) is 62.45 °C, lower than the control IgG SB 4 sb S228P whose Tm1 is 69.00 °C, indicating that PD-1 × LAG3 TiMab is relatively stable." now reads: "The thermostability was also examined with Uncle biologics stability screening platform (Unchained Labs). [Extracted from the article]

β-lactam antibiotics are widely used in clinic. Filamentous fungus Acremonium chrysogenum is an important industrial fungus for the production of CPC, one of the major precursors of β-lactam antibiotics. Although its fermentation yield has been bred significantly over the past decades, little is known regarding molecular changes between the industrial strain and the wild type strain. This limits the possibility to improve CPC production further by molecular breeding. Comparative transcriptome is a powerful tool to understand the molecular mechanisms of CPC industrial high yield producer compared to wild type. A total of 57 million clean sequencing reads with an average length of 100 bp were generated from Illumina sequencing platform. 22,878 sequences were assembled. Among the assembled unigenes, 9502 were annotated and 1989 annotated sequences were assigned to 121 pathways by searching against the Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) database. Furthermore, we compared the transcriptome differences between a high-yield and a wild-type strain during fermentation. A total of 4329 unigenes with significantly different transcription level were identified, among which 1737 were up-regulated and 2592 were down-regulated. 24 pathways were subsequently determined which involve glycerolipid metabolism, galactose metabolism, and pyrimidine metabolism. We also examined the transcription levels of 18 identified genes, including 11 up-regulated genes and 7 down-regulated genes using reverse transcription quantitative -PCR (RT-qPCR). The results of RT-qPCR were consistent with the Illumina sequencing. In this study, the Illumina sequencing provides the most comprehensive sequences for gene expression profile of Acremonium chrysogenum and allows de novo transcriptome assembly while lacking genome information. Comparative analysis of RNA-seq data reveals the complexity of the transcriptome in the fermentation of different yield strains. This is an important public information platform which could be used to accelerate the research to improve CPC production in Acremonium chrysogenum. [ABSTRACT FROM AUTHOR]