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8 results on '"Gu, Zhenning"'

2. TMPO-AS1, a Novel E2F1-Regulated lncRNA, Contributes to the Proliferation of Lung Adenocarcinoma Cells via Modulating miR-326/SOX12 Axis

Author

Wei,Lin, Liu,Yugang, Zhang,Huijun, Ma,Yuefeng, Lu,Zenghui, Gu,Zhenning, and Ding,Chao

Subjects
Cancer Management and Research
Abstract

Lin Wei,1 Yugang Liu,1 Huijun Zhang,1 Yuefeng Ma,2 Zenghui Lu,1 Zhenning Gu,1 Chao Ding1 1Department of Thoracic Surgery, Xi’an Chest Hospital, Xi’an 710100, People’s Republic of China; 2Department of Thoracic Surgery, The Second Affiliated Hospital of Xi’an Jiao Tong University, Xi’an 710004, People’s Republic of ChinaCorrespondence: Chao DingDepartment of Thoracic Surgery, Xi’an Chest Hospital, Xi’an, Shaanxi Province 710100, People’s Republic of ChinaTel/Fax +86-29-62500053Email abroad2014@sohu.comBackground: TMPO-AS1, an antisense lncRNA located at human chromosome 12p23.1, has been identified as an oncogene involved in cell proliferation in various cancers, including LUAD. In this study, we aimed to explore the novel molecular mechanism of TMPO-AS1 underlying LUAD growth.Materials and Methods: The transcription levels of TMPO-AS1, miR-326, and SOX12 in LUAD tissues and cell lines were detected by quantitative real-time PCR (qRT-PCR). The cell proliferation ability was evaluatect 3d by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis analysis was assessed by flow cytometry. The target relationship among TMPO-AS1, miR-326, and SOX12 and promoter activity of TMPO-AS1 was measured using dual-luciferase reporter assay. The protein levels of SOX12 in LUAD cells were determined by Western blot. ChIP-qPCR assay was performed to validate the direct binding between E2F1 and TMPO-AS1 promoter.Results: TMPO-AS1 was up-regulated in LUAD tissues as well as cell lines. Boosted TMPO-AS1 expression was positively correlated with poor prognosis and pathological stage in LUAD. Down-regulation of TMPO-AS1 could restrain the proliferation of LUAD cells through arresting the cell cycle at G0/G1 phase and inducing apoptosis in vitro. Mechanically, we demonstrated that TMPO-AS1 could modulate the proliferation of LUAD cells through increasing SOX12 expression level via sponging miR-326 in accordance with bioinformatics analysis and experimental validation. Furthermore, we identified that TMPO-AS1 could be activated by E2F transcription factor 1 (E2F1) as a novel target gene.Conclusion: TMPO-AS1 can modulate LUAD cell proliferation through E2F1/miR-326/SOX12 pathway.Keywords: lung adenocarcinoma, TMPO-AS1, cell proliferation, miR-326, SOX12, E2F1

Published
2020

3. Identification of large fullerenes formed during the growth of single-walled carbon nanotubes in the HiPco process

Author

Ramesh, Sivarajan, Brinson, Bruce, Margrave, J.L., Johnson, M. Pontier, Gu, Zhenning, Saini, Rajesh K., Willis, Peter, Marriott, Terry, Billups, W.E., Hauge, Robert H., and Smalley, Richard E.

Subjects
Mass spectrometry -- Usage, Nanotubes -- Chemical properties, Chemicals, plastics and rubber industries
Abstract

A small fraction of high-molecular-weight closed shell carbon clusters are formed along with the single-walled carbon nanotubes during the catalytic, high-pressure decomposition of carbon monoxide. The fullerenic clusters and can be desorbed by laser irradiation thus providing for a quantitative estimation by LDI-TOF mass spectrometry with appropriate markers.

Published
2003

6. High Yield Methods for Cutting Single-Walled Carbon Nanotubes

Author

RICE UNIVERSITY HOUSTON TX CENTER FOR NANOSCALE SCIENCE AND TECHNOLOGY, Gu, Zhenning, Ziegler, Kirk J., Schmidt, Howard K., Chen, Zheyi, Rauwald, Urs, Hauge, Robert H., Smalley, Richard E., Tour, James M., RICE UNIVERSITY HOUSTON TX CENTER FOR NANOSCALE SCIENCE AND TECHNOLOGY, Gu, Zhenning, Ziegler, Kirk J., Schmidt, Howard K., Chen, Zheyi, Rauwald, Urs, Hauge, Robert H., Smalley, Richard E., and Tour, James M.

Abstract

Methods for cutting single-walled carbon nanotubes (SWNTs) to controlled lengths for material, biological and electronic applications are being developed. The research aims at establishing scalable cutting processes for preparing high quality short SWNTs with high yield, systematic procedure for evaluating the cutting results, and effective, scalable methods for length sorting of cut SWNTs. Caro's acid has been studied and chosen as the finishing cutting reagent. High temperature fluorination plus Caro's acid have been developed as our primary cutting method. Electron beam irradiation of ozonated SWNTs has shown promising cutting result. A standard procedure has been developed to individualize SWNT samples and to determine the length distribution. Preliminary results have indicated that phase separation technique could be a scalable, efficient means for length sorting of SWNTs. Ultra-short SWNTs (US-SWNTs) were produced by oxidation in oleum using fuming nitric acid., The original document contains color images.

Published
2008

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