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27 results on '"Guillermina Hernandez-Raquet"'

1. Anaerobic lignocellulolytic microbial consortium derived from termite gut: enrichment, lignocellulose degradation and community dynamics

Author: Adèle Lazuka, Lucas Auer, Michael O’Donohue, and Guillermina Hernandez-Raquet
Subjects: Lignocellulose, Anaerobic microbial consortium, Termite gut microbiome, Carboxylates, Xylanase, Cellulase, Fuel, TP315-360, Biotechnology, TP248.13-248.65
Abstract: Abstract Background Lignocellulose is the most abundant renewable carbon resource that can be used for biofuels and commodity chemicals production. The ability of complex microbial communities present in natural environments that are specialized in biomass deconstruction can be exploited to develop lignocellulose bioconversion processes. Termites are among the most abundant insects on earth and play an important role in lignocellulose decomposition. Although their digestive microbiome is recognized as a potential reservoir of microorganisms producing lignocellulolytic enzymes, the potential to enrich and maintain the lignocellulolytic activity of microbial consortia derived from termite gut useful for lignocellulose biorefinery has not been assessed. Here, we assessed the possibility of enriching a microbial consortium from termite gut and maintaining its lignocellulose degradation ability in controlled anaerobic bioreactors. Results We enriched a termite gut-derived consortium able to transform lignocellulose into carboxylates under anaerobic conditions. To assess the impact of substrate natural microbiome on the enrichment and the maintenance of termite gut microbiome, the enrichment process was performed using both sterilized and non-sterilized straw. The enrichment process was carried out in bioreactors operating under industrially relevant aseptic conditions. Two termite gut-derived microbial consortia were obtained from Nasutitermes ephratae by sequential batch culture on raw wheat straw as the sole carbon source. Analysis of substrate loss, carboxylate production and microbial diversity showed that regardless of the substrate sterility, the diversity of communities selected by the enrichment process strongly changed compared to that observed in the termite gut. Nevertheless, the community obtained on sterile straw displayed higher lignocellulose degradation capacity; it showed a high xylanase activity and an initial preference for hemicellulose. Conclusions This study demonstrates that it is possible to enrich and maintain a microbial consortium derived from termite gut microbiome in controlled anaerobic bioreactors, producing useful carboxylates from raw biomass. Our results suggest that the microbial community is shaped both by the substrate and the conditions that prevail during enrichment. However, when aseptic conditions are applied, it is also affected by the biotic pressure exerted by microorganisms naturally present in the substrate and in the surrounding environment. Besides the efficient lignocellulolytic consortium enriched in this study, our results revealed high levels of xylanase activity that can now be further explored for enzyme identification and overexpression for biorefinery purposes.
Published: 2018
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2. Screening of Phytophagous and Xylophagous Insects Guts Microbiota Abilities to Degrade Lignocellulose in Bioreactor

Author: Amandine Gales, Lucile Chatellard, Maider Abadie, Anaïs Bonnafous, Lucas Auer, Hélène Carrère, Jean-Jacques Godon, Guillermina Hernandez-Raquet, and Claire Dumas
Subjects: fermentation, biomimetism, insect guts, volatile fatty acids, microbial diversity, Microbiology, QR1-502
Abstract: Microbial consortia producing specific enzymatic cocktails are present in the gut of phytophagous and xylophagous insects; they are known to be the most efficient ecosystems to degrade lignocellulose. Here, the ability of these consortia to degrade ex vivo lignocellulosic biomass in anaerobic bioreactors was characterized in term of bioprocess performances, enzymatic activities and bacterial community structure. In a preliminary screening, guts of Ergates faber (beetle), Potosia cuprea (chafer), Gromphadorrhina portentosa (cockroach), Locusta migratoria (locust), and Gryllus bimaculatus (cricket) were inoculated in anaerobic batch reactors, in presence of grounded wheat straw at neutral pH. A short duration fermentation of less than 8 days was observed and was related to a drop of pH from 7 to below 4.5, leading to an interruption of gas and metabolites production. Consistently, a maximum of 180 mgeq.COD of metabolites accumulated in the medium, which was related to a low degradation of the lignocellulosic biomass, with a maximum of 5 and 2.2% observed for chafer and locust gut consortia. The initial cell-bound and extracellular enzyme activities, i.e., xylanase and β-endoglucanase, were similar to values observed in the literature. Wheat straw fermentation in bioreactors leads to an increase of cell-bounded enzyme activities, with an increase of 145% for cockroach xylanase activity. Bacterial community structures were insect dependent and mainly composed of Clostridia, Bacteroidia and Gammaproteobacteria. Improvement of lignocellulose biodegradation was operated in successive batch mode at pH 8 using the most interesting consortia, i.e., locust, cockroaches and chafer gut consortia. In these conditions, lignocellulose degradation increased significantly: 8.4, 10.5, and 21.0% of the initial COD were degraded for chafer, cockroaches and locusts, respectively in 15 days. Consistently, xylanase activity tripled for the three consortia, attesting the improvement of the process. Bacteroidia was the major bacterial class represented in the bacterial community for all consortia, followed by Clostridia and Gammaproteobacteria classes. This work demonstrates the possibility to maintain apart of insect gut biological activity ex vivo and shows that lignocellulose biodegradation can be improved by using a biomimetic approach. These results bring new insights for the optimization of lignocellulose degradation in bioreactors.
Published: 2018
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3. Uncovering the Potential of Termite Gut Microbiome for Lignocellulose Bioconversion in Anaerobic Batch Bioreactors

Author: Lucas Auer, Adèle Lazuka, David Sillam-Dussès, Edouard Miambi, Michael O'Donohue, and Guillermina Hernandez-Raquet
Subjects: termite, gut-microbiome, lignocellulose bioconversion, carboxylate production, microbial diversity, Microbiology, QR1-502
Abstract: Termites are xylophages, being able to digest a wide variety of lignocellulosic biomass including wood with high lignin content. This ability to feed on recalcitrant plant material is the result of complex symbiotic relationships, which involve termite-specific gut microbiomes. Therefore, these represent a potential source of microorganisms for the bioconversion of lignocellulose in bioprocesses targeting the production of carboxylates. In this study, gut microbiomes of four termite species were studied for their capacity to degrade wheat straw and produce carboxylates in controlled bioreactors. All of the gut microbiomes successfully degraded lignocellulose and up to 45% w/w of wheat straw degradation was observed, with the Nasutitermes ephratae gut-microbiome displaying the highest levels of wheat straw degradation, carboxylate production and enzymatic activity. Comparing the 16S rRNA gene diversity of the initial gut inocula to the bacterial communities in lignocellulose degradation bioreactors revealed important changes in community diversity. In particular, taxa such as Spirochaetes and Fibrobacteres that were highly abundant in the initial gut inocula were replaced by Firmicutes and Proteobacteria at the end of incubation in wheat straw bioreactors. Overall, this study demonstrates that termite-gut microbiomes constitute a reservoir of lignocellulose-degrading bacteria that can be harnessed in artificial conditions for biomass conversion processes that lead to the production of useful molecules.
Published: 2017
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4. Impact of phosphorus limitation on medium-chain-length polyhydroxyalkanoate production by activated sludge

Author: Emilie Alaux, Bastien Marie, Marion Couvreur, Mansour Bounouba, Guillermina Hernandez-Raquet, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BIOPLAST Interreg POCTEFA project EFA253/16, ECOPLAST project 20009834, and Région Occitanie
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Mixed culturesBacterial diversity, [SDV]Life Sciences [q-bio], Phosphorus limitation, General Medicine, Medium-chain-length polyhydroxyalkanoates, Applied Microbiology and Biotechnology, Bioplastics, Biotechnology
Abstract: International audience; For a sustainable economy, biodegradable biopolymers polyhydroxyalkanoates (PHA) are desirable substitutes to petroleum-based plastics that contaminate our environment. Medium-chain-length (MCL) PHA bioplastics are particularly interesting due to their thermoplastic properties. To hamper the high cost associated to PHA production, the use of bacterial mixed cultures cultivated in open systems and using cheap resources is a promising strategy. Here, we studied the operating conditions favouring direct MCL accumulation by activated sludge, using oleic acid as a model substrate and phosphorus limitation in fed-batch bioreactors. Our results confirm the presence of PHA-accumulating organisms (PHAAO) in activated sludge able to accumulate MCL from oleic acid. A positive correlation between phosphorus (P) limitation and PHA accumulation was demonstrated, allowing up to 26% PHA/total biomass accumulation, and highlighted its negative impact on the MCL/PHA fraction in the polymer. Diversity analysis through 16S rRNA amplicon sequencing revealed a differential selection of PHAAO according to the P-limitation level. A differential behaviour for the orders Pseudomonadales and Burkholderiales at increasing P-limitation levels was revealed, with a higher abundance of the latter at high levels of P-limitation. The PHA accumulation observed in activated sludge open new perspectives for MCL-PHA production system based on P-limitation strategy applied to mixed microbial communities.
Published: 2023
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5. Termite Gut Microbiota Contribution to Wheat Straw Delignification in Anaerobic Bioreactors

Author: Gijs van Erven, Pui Ying Lam, Guillermina Hernandez-Raquet, Fabien Mounet, Jacqueline Grima-Pettenati, Mirjam A. Kabel, Yuki Tobimatsu, Louison Dumond, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Kyoto University [Kyoto], Wageningen University and Research [Wageningen] (WUR), Régulation et Dynamique de la Formation du Bois, Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), French National Institute of Research for Agriculture, Food and Environment (INRAE), Region Occitanie (31000553), Carnot Institute 3BCAR-Insyme project, PHC Sakura program from Campus France (45094PM), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Kyoto University, Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, General Chemical Engineering, 02 engineering and technology, Gut flora, Termite gut microbiome, 010402 general chemistry, 01 natural sciences, complex mixtures, Lignin, chemistry.chemical_compound, NMR spectroscopy, Anaerobic digestion, Levensmiddelenchemie, Bioreactor, Environmental Chemistry, biology, Food Chemistry, Renewable Energy, Sustainability and the Environment, Chemistry, py-GC-MS, technology, industry, and agriculture, food and beverages, General Chemistry, Straw, 021001 nanoscience & nanotechnology, biology.organism_classification, Pulp and paper industry, 0104 chemical sciences, Delignification, BBP Biorefinery & Sustainable Value Chains, 0210 nano-technology, Anaerobic exercise, Lignocellulose
Abstract: Lignin is a major lock for lignocellulose valorizationin biorefineries, prompting a need tofind newligninolytic systems. Termites are efficient lignocellulose degraders, and a large part ofthis ability comes from its anaerobic gut microbiome. However, thepotential of termite gut microbiomes to degrade lignin underanaerobicconditions has yet to be elucidated. By applying wetchemistry, multidimensional NMR spectroscopy, and quantitative13C-IS py-GC-MS, we determined the chemical and structuralcharacteristics of wheat straw digested by the gut microbiomes ofhigher termitesNasutitermes ephratae,Nasutitermes lujae,Micro- cerotermes parvus, andTermes hospesimplemented in anaerobicbioreactors. Interestingly, all gut microbiomes managed to removelignin (up to 37%), although hemicellulose (mean 51%) andcellulose(mean 41%) were degraded more efficiently. Important structural differences, indicative of ligninolytic action, were discerned in the residual lignin. For the studied termite gut microbiomes, a slight decrease of S/G ratio was observed upon digestion, whereas lignin’s interunit linkages and Cα-oxidized moieties, including benzaldehyde and hydroxypropiovanillone/syringone substructures, accumulated. Additionally, tricin terminal units were clearly depleted, along with decreases inp-coumarate and ferulate pendant units. At least 80% of the observed delignification is estimated to be due to the removal of“true”lignin, with the remainder being explained by the removal of hydroxycinnamic acids and tricin. Collectively, ourfindings suggest a partial deconstruction of lignin, with“peripheral”lignin subunits being preferentially targeted. The present work thus provides new insights into the anaerobic deconstruction of lignin by termite gut microbiomes.
Published: 2021
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6. Enrichment and adaptation yield high anammox conversion rates under low temperatures

Author: M. Caligaris, Guillermina Hernandez-Raquet, G. Gaval, Siegfried E. Vlaeminck, Mathieu Spérandio, B. Barillon, S. Dubos, Yolaine Bessiere, I. Mozo, P. De Cocker, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), CIRSEE, Suez, Center for Microbial Ecology and Technology (CMET), Ghent University, Treatment Infrastructures, Research Group of Sustainable Energy, Air and Water Technology, University of Antwerp, ANRT (CIFRE) [2014/0754], Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche Sur l'Eau et l'Environnement [Suez] (CIRSEE), SUEZ ENVIRONNEMENT (FRANCE), Universiteit Gent = Ghent University (UGENT), University of Antwerp (UA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), and Universiteit Gent = Ghent University [Belgium] (UGENT)
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Environmental Engineering, Denitrification, Nitrogen, Microorganism, 0208 environmental biotechnology, chemistry.chemical_element, bioréacteur, Bioengineering, 02 engineering and technology, 010501 environmental sciences, Biology, 01 natural sciences, chemistry.chemical_compound, Bioreactors, Ammonium Compounds, séquençage, Bioreactor, Ammonium, biological nitrogen removal, Waste Management and Disposal, shortcut nitrogen removal, 0105 earth and related environmental sciences, bactérie, Renewable Energy, Sustainability and the Environment, Physics, Temperature, Environmental engineering, General Medicine, bacterium, cold anammox, Anoxic waters, réacteur anaérobie, 020801 environmental engineering, Activated sludge, chemistry, microbial community structure, Anammox, Oxidation-Reduction, Engineering sciences. Technology, Nuclear chemistry
Abstract: This study compared two anammox sequencing batch reactors (SBR) for one year. SBRconstantT was kept at 30 degrees C while temperature in SBRloweringT was decreased step-wise from 30 degrees C to 20 degrees C and 15 degrees C followed by over 140 days at 12.5 degrees C and 10 degrees C. High retention of anammox bacteria (AnAOB) and minimization of competition with AnAOB were key. 5-L anoxic reactors with the same inoculum were fed synthetic influent containing 25.9 mg NH4+-N/L and 34.1 mg NO2--N/L (no COD). Specific ammonium removal rates continuously increased in SBRconstantT, reaching 785 mg NH4+-N/gVSS/d, and were maintained in SBRloweringT, reaching 82.2 and 91.8 mg NH4+-N/gVSS/d at 12.5 and 10 degrees C respectively. AnAOB enrichment (increasing hzsA and 16S rDNA gene concentrations) and adaptation (shift from Ca. Brocadia to Ca. Kuenenia in SBRloweringT) contributed to these high rates. Rapidly settling granules developed, with average diameters of 1.2 (SBRconstantT) and 1.6 mm (SBRloweringT). Results reinforce the potential of anammox for mainstream applications.
Published: 2018
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7. Avis en réponse à la saisine HCB - dossier EFSA-GMO-RX-013. Paris, le 30 janvier 2019

Author: Comité Scientifique Du Haut Conseil Des Biotechnologies, Frédérique Angevin, Claude Bagnis, Avner Bar-Hen, Marie-Anne Barny, Pascal Boireau, Thierry Brévault, Bruno Chauvel, Cécile Collonnier, Denis Couvet, Elie Dassa, Hubert de Verneuil, Barbara Demeneix, Claudine Franche, Philippe Guerche, Joël Guillemain, Guillermina Hernandez Raquet, Jamal Khalife, Bernard Klonjkowski, Marc Lavielle, Valérie Le Corre, François Lefèvre, Olivier Lemaire, Didier Lereclus, Rémy Maximilien, Eliane Meurs, Nadia Naffakh, Didier NEGRE, Jean-Louis Noyer, Sergio Ochatt, Jean-Christophe Pages, Xavier Raynaud, Catherine Regnault-Roger, Michel Renard, Tristan Renault, Patrick Saindrenan, Pascal Simonet, Marie-Bérengère Troadec, Bernard Vaissière, Jean Luc Vilotte, ProdInra, Migration, Haut Conseil des Biotechnologies (HCB), Unité Impacts Ecologiques des Innovations en Production Végétale (ECO-INNOV), Institut National de la Recherche Agronomique (INRA), Etablissement Français du Sang, Université Paris-Sud - Paris 11 (UP11), Université Paris Descartes - Paris 5 (UPD5), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris), Institut National de la Recherche Agronomique (INRA)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Direction des Ressources Humaines et du Développement Durable (DRHDD), Agroécologie et Intensification Durables des cultures annuelles (UPR AIDA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Muséum national d'Histoire naturelle (MNHN), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche pour le Développement (IRD), Agence nationale de sécurité du médicament et des produits de santé [Saint-Denis] (ANSM), Université Francois Rabelais [Tours], Toulouse Biotechnology Institute (TBI), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Modélisation en pharmacologie de population (XPOP), Centre de Mathématiques Appliquées - Ecole Polytechnique (CMAP), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Pasteur [Paris] (IP), Cirad Direction Générale (Cirad-DG), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Abeilles et environnement (AE), Génétique Animale et Biologie Intégrative (GABI), Haut Conseil des Biotechnologies, Commanditaire : Ministère de l'Agriculture, de l'Agroalimentaire et de la Forêt (France), Type de commande : Commande avec contrat/convention/lettre de saisine, Type de commanditaire ou d'auteur de la saisine : Ministères, parlements et les structures qui leur sont directement rattachées, Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Direction des Ressources Humaines et du Développement Durable, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), AgroParisTech-Institut National de la Recherche Agronomique (INRA), and Université de Strasbourg (UNISTRA)-Institut National de la Recherche Agronomique (INRA)
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SHS] Humanities and Social Sciences, [SDV.IDA] Life Sciences [q-bio]/Food engineering, [SHS]Humanities and Social Sciences
Published: 2019

8. Avis en réponse à la saisine HCB - EFSA-GMO-ES-2018-154. Paris, le 5 avril 2019

Author: Comité Scientifique Du Haut Conseil Des Biotechnologies, Frédérique Angevin, Claude Bagnis, Avner Bar-Hen, Marie-Anne Barny, Pascal Boireau, Thierry Brévault, Bruno Chauvel, Cécile Collonnier, Denis Couvet, Elie Dassa, Hubert de Verneuil, Barbara Demeneix, Claudine Franche, Philippe Guerche, Joël Guillemain, Guillermina Hernandez Raquet, Jamal Khalife, Bernard Klonjkowski, Marc Lavielle, Valérie Le Corre, François Lefèvre, Olivier Lemaire, Didier Lereclus, Rémy Maximilien, Eliane Meurs, Nadia Naffakh, Didier NEGRE, Jean-Louis Noyer, Sergio Ochatt, Jean-Christophe Pages, Xavier Raynaud, Catherine Regnault-Roger, Michel Renard, Tristan Renault, Patrick Saindrenan, Pascal Simonet, Marie-Bérengère Troadec, Bernard Vaissière, Jean Luc Vilotte, Haut Conseil des Biotechnologies (HCB), Unité Impacts Ecologiques des Innovations en Production Végétale (ECO-INNOV), Institut National de la Recherche Agronomique (INRA), Etablissement Français du Sang [La Plaine Saint-Denis] (EFS), Université Paris-Sud - Paris 11 (UP11), Université Paris Descartes - Paris 5 (UPD5), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris), Institut National de la Recherche Agronomique (INRA)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Direction des Ressources Humaines et du Développement Durable (DRHDD), Agroécologie et Intensification Durables des cultures annuelles (UPR AIDA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Muséum national d'Histoire naturelle (MNHN), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche pour le Développement (IRD), Agence nationale de sécurité du médicament et des produits de santé [Saint-Denis] (ANSM), Université Francois Rabelais [Tours], Toulouse Biotechnology Institute (TBI), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Modélisation en pharmacologie de population (XPOP), Centre de Mathématiques Appliquées - Ecole Polytechnique (CMAP), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Pasteur [Paris] (IP), Cirad Direction Générale (Cirad-DG), Etablissement Français du Sang, EFS, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Abeilles et Environnement (AE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Animale et Biologie Intégrative (GABI), Haut Conseil des Biotechnologies, Commanditaire : Ministère de l'Agriculture, de l'Agroalimentaire et de la Forêt (France), Type de commande : Commande avec contrat/convention/lettre de saisine, Type de commanditaire ou d'auteur de la saisine : Ministères, parlements et les structures qui leur sont directement rattachées, Direction des Ressources Humaines et du Développement Durable, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), AgroParisTech-Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), and Université de Strasbourg (UNISTRA)-Institut National de la Recherche Agronomique (INRA)
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SHS] Humanities and Social Sciences, [SDV.IDA] Life Sciences [q-bio]/Food engineering, [SHS]Humanities and Social Sciences
Published: 2019

9. Avis en réponse à la saisine HCB - dossier RX-015. Paris, le 26 février 2019

Author: Comité Scientifique Du Haut Conseil Des Biotechnologies, Frédérique Angevin, Claude Bagnis, Avner Bar-Hen, Marie-Anne Barny, Pascal Boireau, Thierry Brévault, Bruno Chauvel, Cécile Collonnier, Denis Couvet, Elie Dassa, Hubert de Verneuil, Barbara Demeneix, Claudine Franche, Philippe Guerche, Joël Guillemain, Guillermina Hernandez Raquet, Jamal Khalife, Bernard Klonjkowski, Marc Lavielle, Valérie Le Corre, François Lefèvre, Olivier Lemaire, Didier Lereclus, Rémy Maximilien, Eliane Meurs, Nadia Naffakh, Didier NEGRE, Jean-Louis Noyer, Sergio Ochatt, Jean-Christophe Pages, Xavier Raynaud, Catherine Regnault-Roger, Michel Renard, Tristan Renault, Patrick Saindrenan, Pascal Simonet, Marie-Bérengère Troadec, Bernard Vaissière, Jean Luc Vilotte, Haut Conseil des Biotechnologies (HCB), Unité Impacts Ecologiques des Innovations en Production Végétale (ECO-INNOV), Institut National de la Recherche Agronomique (INRA), Etablissement Français du Sang, Université Paris-Sud - Paris 11 (UP11), Université Paris Descartes - Paris 5 (UPD5), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris), Institut National de la Recherche Agronomique (INRA)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Direction des Ressources Humaines et du Développement Durable (DRHDD), Agroécologie et Intensification Durables des cultures annuelles (UPR AIDA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Muséum national d'Histoire naturelle (MNHN), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche pour le Développement (IRD), Agence nationale de sécurité du médicament et des produits de santé [Saint-Denis] (ANSM), Université Francois Rabelais [Tours], Toulouse Biotechnology Institute (TBI), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Modélisation en pharmacologie de population (XPOP), Centre de Mathématiques Appliquées - Ecole Polytechnique (CMAP), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Pasteur [Paris] (IP), Cirad Direction Générale (Cirad-DG), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Abeilles et environnement (AE), Génétique Animale et Biologie Intégrative (GABI), Haut Conseil des Biotechnologies, Commanditaire : Ministère de l'Agriculture, de l'Agroalimentaire et de la Forêt (France), Type de commande : Commande avec contrat/convention/lettre de saisine, Type de commanditaire ou d'auteur de la saisine : Ministères, parlements et les structures qui leur sont directement rattachées, Direction des Ressources Humaines et du Développement Durable, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Université de Strasbourg (UNISTRA)-Institut National de la Recherche Agronomique (INRA), Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SHS] Humanities and Social Sciences, [SDV.IDA] Life Sciences [q-bio]/Food engineering, [SHS]Humanities and Social Sciences
Published: 2019

10. Avis en réponse à la saisine HCB - dossier 2018-150. Paris, le 24 octobre 2018

Author: Comité Scientifique Du Haut Conseil Des Biotechnologies, Frédérique Angevin, Claude Bagnis, Avner Bar-Hen, Marie-Anne Barny, Pascal Boireau, Thierry Brévault, Bruno Chauvel, Cécile Collonnier, Denis Couvet, Elie Dassa, Hubert de Verneuil, Barbara Demeneix, Claudine Franche, Philippe Guerche, Joël Guillemain, Guillermina Hernandez Raquet, Jamal Khalife, Bernard Klonjkowski, Marc Lavielle, Valérie Le Corre, François Lefèvre, Olivier Lemaire, Didier Lereclus, Rémy Maximilien, Eliane Meurs, Nadia Naffakh, Didier NEGRE, Jean-Louis Noyer, Sergio Ochatt, Jean-Christophe Pages, Xavier Raynaud, Catherine Regnault-Roger, Michel Renard, Tristan Renault, Patrick Saindrenan, Pascal Simonet, Marie-Bérengère Troadec, Bernard Vaissière, Jean Luc Vilotte, Haut Conseil des Biotechnologies (HCB), Unité Impacts Ecologiques des Innovations en Production Végétale (ECO-INNOV), Institut National de la Recherche Agronomique (INRA), Etablissement Français du Sang, Université Paris-Sud - Paris 11 (UP11), Université Paris Descartes - Paris 5 (UPD5), Institut d'écologie et des sciences de l'environnement de Paris (iEES), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Direction des Ressources Humaines et du Développement Durable (DRHDD), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Muséum national d'Histoire naturelle (MNHN), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche pour le Développement (IRD), Agence nationale de sécurité du médicament et des produits de santé [Saint-Denis] (ANSM), Université Francois Rabelais [Tours], Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Modélisation en pharmacologie de population (XPOP), Centre de Mathématiques Appliquées - Ecole Polytechnique (CMAP), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Pasteur [Paris] (IP), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Abeilles et environnement (AE), Génétique Animale et Biologie Intégrative (GABI), Commanditaire : Ministère de l'Agriculture, de l'Agroalimentaire et de la Forêt (France), Type de commande : Commande avec contrat/convention/lettre de saisine, Type de commanditaire ou d'auteur de la saisine : Ministères, parlements et les structures qui leur sont directement rattachées, Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), Direction des Ressources Humaines et du Développement Durable, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, Université de Strasbourg (UNISTRA)-Institut National de la Recherche Agronomique (INRA), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SHS] Humanities and Social Sciences, [SDV.IDA] Life Sciences [q-bio]/Food engineering, [SHS]Humanities and Social Sciences
Published: 2018

11. Avis en réponse à la saisine HCB - dossier 2017-143. Paris, le 15 mai 2018

Author: Comité Scientifique Du Haut Conseil Des Biotechnologies, Frédérique Angevin, Claude Bagnis, Avner Bar-Hen, Marie-Anne Barny, Pascal Boireau, Thierry Brévault, Bruno Chauvel, Cécile Collonnier, Denis Couvet, Elie Dassa, Hubert de Verneuil, Barbara Demeneix, Claudine Franche, Philippe Guerche, Joël Guillemain, Guillermina Hernandez Raquet, Jamal Khalife, Bernard Klonjkowski, Marc Lavielle, Valérie Le Corre, François Lefèvre, Olivier Lemaire, Didier Lereclus, Rémy Maximilien, Eliane Meurs, Nadia Naffakh, Didier NEGRE, Jean-Louis Noyer, Sergio Ochatt, Jean-Christophe Pages, Xavier Raynaud, Catherine Regnault-Roger, Michel Renard, Tristan Renault, Patrick Saindrenan, Pascal Simonet, Marie-Bérengère Troadec, Bernard Vaissière, Jean Luc Vilotte, Haut Conseil des Biotechnologies (HCB), Unité Impacts Ecologiques des Innovations en Production Végétale (ECO-INNOV), Institut National de la Recherche Agronomique (INRA), Etablissement Français du Sang, Université Paris-Sud - Paris 11 (UP11), Université Paris Descartes - Paris 5 (UPD5), Institut d'écologie et des sciences de l'environnement de Paris (iEES), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Direction des Ressources Humaines et du Développement Durable (DRHDD), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Muséum national d'Histoire naturelle (MNHN), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche pour le Développement (IRD), Agence nationale de sécurité du médicament et des produits de santé [Saint-Denis] (ANSM), Université Francois Rabelais [Tours], Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Modélisation en pharmacologie de population (XPOP), Centre de Mathématiques Appliquées - Ecole Polytechnique (CMAP), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Pasteur [Paris] (IP), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Abeilles et environnement (AE), Génétique Animale et Biologie Intégrative (GABI), Commanditaire : Ministère de l'Agriculture, de l'Agroalimentaire et de la Forêt (France), Type de commande : Commande avec contrat/convention/lettre de saisine, Type de commanditaire ou d'auteur de la saisine : Ministères, parlements et les structures qui leur sont directement rattachées, Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), Direction des Ressources Humaines et du Développement Durable, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, Université de Strasbourg (UNISTRA)-Institut National de la Recherche Agronomique (INRA), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)
Subjects: [SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SHS] Humanities and Social Sciences, [SDV.IDA] Life Sciences [q-bio]/Food engineering, [SHS]Humanities and Social Sciences
Published: 2018

12. Screening of Phytophagous and Xylophagous insects guts microbiota abilities to degrade lignocellulose in bioreactor

Author: Anaïs Bonnafous, Guillermina Hernandez-Raquet, Maider Abadie, Hélène Carrère, Jean-Jacques Godon, Lucas Auer, Amandine Galès, Lucile Chatellard, Claire Dumas, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Carnot Institute 3BCAR – Insyme project, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Hernandez Raquet, Guillermina, and Dumas, Claire
Subjects: 0301 basic medicine, Microbiology (medical), biomimetism, biomimicry, [SDV]Life Sciences [q-bio], 030106 microbiology, lcsh:QR1-502, Lignocellulosic biomass, acide gras volatil, 7. Clean energy, Microbiology, lcsh:Microbiology, dégradation de la lignocellulose, Clostridia, 03 medical and health sciences, biomimétisme, diversité microbienne, Gammaproteobacteria, Bioreactor, Food science, Bioprocess, fermentation, bioréacteur anaérobie, Original Research, 2. Zero hunger, insecte xylophage, biology, Chemistry, volatile fatty acids, food and beverages, insect guts, microbial diversity, Biodegradation, biology.organism_classification, timber boring insects, 030104 developmental biology, Xylanase, Fermentation, volatile fatty acid, insecte phytophage
Abstract: Microbial consortia producing specific enzymatic cocktails are present in the gut of phytophagous and xylophagous insects; they are known to be the most efficient ecosystems to degrade lignocellulose. Here, the ability of these consortia to degrade ex vivo lignocellulosic biomass in anaerobic bioreactors was characterized in term of bioprocess performances, enzymatic activities and bacterial community structure. In a preliminary screening, guts of Ergates faber (beetle), Potosia cuprea (chafer), Gromphadorrhina portentosa (cockroach), Locusta migratoria (locust), and Gryllus bimaculatus (cricket) were inoculated in anaerobic batch reactors, in presence of grounded wheat straw at neutral pH. A short duration fermentation of less than 8 days was observed and was related to a drop of pH from 7 to below 4.5, leading to an interruption of gas and metabolites production. Consistently, a maximum of 180 mgeq.COD of metabolites accumulated in the medium, which was related to a low degradation of the lignocellulosic biomass, with a maximum of 5 and 2.2% observed for chafer and locust gut consortia. The initial cell-bound and extracellular enzyme activities, i.e., xylanase and beta-endoglucanase, were similar to values observed in the literature. Wheat straw fermentation in bioreactors leads to an increase of cell-bounded enzyme activities, with an increase of 145% for cockroach xylanase activity. Bacterial community structures were insect dependent and mainly composed of Clostridia, Bacteroidia and Gammaproteobacteria. Improvement of lignocellulose biodegradation was operated in successive batch mode at pH 8 using the most interesting consortia, i.e., locust, cockroaches and chafer gut consortia. In these conditions, lignocellulose degradation increased significantly: 8.4, 10.5, and 21.0% of the initial COD were degraded for chafer, cockroaches and locusts, respectively in 15 days. Consistently, xylanase activity tripled for the three consortia, attesting the improvement of the process. Bacteroidia was the major bacterial class represented in the bacterial community for all consortia, followed by Clostridia and Gammaproteobacteria classes. This work demonstrates the possibility to maintain apart of insect gut biological activity ex vivo and shows that lignocellulose biodegradation can be improved by using a biomimetic approach. These results bring new insights for the optimization of lignocellulose degradation in bioreactors.
Published: 2018
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13. Anaerobic lignocellulolytic microbial consortium derived from termite gut: enrichment, lignocellulose degradation and community dynamics

Author: Lucas Auer, Guillermina Hernandez-Raquet, Michael J. O’Donohue, Adèle Lazuka, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Region Languedoc-Roussillon Midi-Pyrenees [31000553], French National Agency for Energy and Environment (ADEME) [TEZ 12-02], Carnot Institute 3BCAR, French National Institute for Agronomical Research-INRA MetaScreen project, Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Bioconversion, Microorganism, lcsh:Biotechnology, Biomass, lignocellulose, anaerobic microbial consortium, termite gut microbiome, carboxylates, xylanase, cellulase, microbiome, bioréacteur, Biotechnologies, Management, Monitoring, Policy and Law, 7. Clean energy, Applied Microbiology and Biotechnology, lcsh:Fuel, 03 medical and health sciences, lcsh:TP315-360, lcsh:TP248.13-248.65, Bioreactor, biomasse, Food science, Microbiome, 2. Zero hunger, Renewable Energy, Sustainability and the Environment, Chemistry, Research, food and beverages, 15. Life on land, Microbial consortium, Biorefinery, 030104 developmental biology, General Energy, Microbial population biology, 13. Climate action, biocarburant, termite, Biotechnology
Abstract: Background Lignocellulose is the most abundant renewable carbon resource that can be used for biofuels and commodity chemicals production. The ability of complex microbial communities present in natural environments that are specialized in biomass deconstruction can be exploited to develop lignocellulose bioconversion processes. Termites are among the most abundant insects on earth and play an important role in lignocellulose decomposition. Although their digestive microbiome is recognized as a potential reservoir of microorganisms producing lignocellulolytic enzymes, the potential to enrich and maintain the lignocellulolytic activity of microbial consortia derived from termite gut useful for lignocellulose biorefinery has not been assessed. Here, we assessed the possibility of enriching a microbial consortium from termite gut and maintaining its lignocellulose degradation ability in controlled anaerobic bioreactors. Results We enriched a termite gut-derived consortium able to transform lignocellulose into carboxylates under anaerobic conditions. To assess the impact of substrate natural microbiome on the enrichment and the maintenance of termite gut microbiome, the enrichment process was performed using both sterilized and non-sterilized straw. The enrichment process was carried out in bioreactors operating under industrially relevant aseptic conditions. Two termite gut-derived microbial consortia were obtained from Nasutitermes ephratae by sequential batch culture on raw wheat straw as the sole carbon source. Analysis of substrate loss, carboxylate production and microbial diversity showed that regardless of the substrate sterility, the diversity of communities selected by the enrichment process strongly changed compared to that observed in the termite gut. Nevertheless, the community obtained on sterile straw displayed higher lignocellulose degradation capacity; it showed a high xylanase activity and an initial preference for hemicellulose. Conclusions This study demonstrates that it is possible to enrich and maintain a microbial consortium derived from termite gut microbiome in controlled anaerobic bioreactors, producing useful carboxylates from raw biomass. Our results suggest that the microbial community is shaped both by the substrate and the conditions that prevail during enrichment. However, when aseptic conditions are applied, it is also affected by the biotic pressure exerted by microorganisms naturally present in the substrate and in the surrounding environment. Besides the efficient lignocellulolytic consortium enriched in this study, our results revealed high levels of xylanase activity that can now be further explored for enzyme identification and overexpression for biorefinery purposes. Electronic supplementary material The online version of this article (10.1186/s13068-018-1282-x) contains supplementary material, which is available to authorized users.
Published: 2018
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14. FROGS: Find, Rapidly, OTUs with Galaxy Solution

Author: Mahendra Mariadassou, Katia Vidal, Sarah Maman, Frédéric Escudié, Géraldine Pascal, Laurent Cauquil, Lucas Auer, Maria Bernard, Guillermina Hernandez-Raquet, Sylvie Combes, Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de la Recherche Agronomique (INRA), Interactions Arbres-Microorganismes (IAM), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Statistics and Probability, Computer science, [SDV]Life Sciences [q-bio], 030106 microbiology, mothur, blast, computer.software_genre, Biochemistry, diversity, 03 medical and health sciences, platform, Microbial ecology, Cluster Analysis, read, rare biosphere, [INFO]Computer Science [cs], [MATH]Mathematics [math], Molecular Biology, RNA gene database, wrinkle, [SDV.GEN]Life Sciences [q-bio]/Genetics, Bacteria, sequencing data, pipeline, Amplicon, Computer Science Applications, Computational Mathematics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, 030104 developmental biology, Computational Theory and Mathematics, Metagenomics, Data mining, computer, Software
Abstract: International audience; Motivation: Metagenomics leads to major advances in microbial ecology and biologists need user friendly tools to analyze their data on their own. Results: This Galaxy-supported pipeline, called FROGS, is designed to analyze large sets of amplicon sequences and produce abundance tables of Operational Taxonomic Units (OTUs) and their taxonomic affiliation. The clustering uses Swarm. The chimera removal uses VSEARCH, combined with original cross-sample validation. The taxonomic affiliation returns an innovative multi-affiliation output to highlight databases conflicts and uncertainties. Statistical results and numerous graphical illustrations are produced along the way to monitor the pipeline. FROGS was tested for the detection and quantification of OTUs on real and in silico datasets and proved to be rapid, robust and highly sensitive. It compares favorably with the widespread mothur, UPARSE and QIIME.; Avec le séquençage haut-débit (454, MiSeq), les pipelines de traitements de séquençage d'ARNr 16S actuels produisent de gros jeu de données. Le calcul des tables d'abondances d'OTU et leur affiliation taxonomique peinent à s'exécuter et les solutions les plus efficaces sont souvent dédiées aux spécialistes. Dans ce contexte, nous avons développé le pipeline "FROGS" : « Find Rapidly OTU with Galaxy Solution ». Développé sous environnement Galaxy, "FROGS" intègre un outil de prétraitement des données permettant le contigage de séquences, le nettoyage des données et la suppression des chimères. La déréplication des séquences se fait par un script python. L'étape de clusterisation s'effectue avec le logiciel SWARM. Un outil d'assignation retourne l’affiliation taxonomique de chaque OTU en utilisant à la fois l'algorithme RDPClassifier et NCBI blast+ sur la banque de données Silva SSU 119. Enfin, un outil de post-traitement permet aux utilisateurs de traiter la table d'abondance obtenue avec les filtres habituels, fournit des résultats statistiques et des illustrations graphiques. FROGS a été développé pour traiter de grandes quantités de données par l'utilisation d’outils innovants et une conception optimisée. De plus, il est portable sur toutes les plateformes Galaxy avec le minimum de dépendances informatiques et architecturales. FROGS a été testé sur plusieurs jeux de données simulées. L'outil se montre extrêmement rapide, robuste et hautement sensible pour la détection des OTU avec très peu de faux-positifs comparé aux autres pipelines largement utilisés par la communauté.
Published: 2018
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15. Ecofriendly lignocellulose pretreatment to enhance the carboxylate production of a rumen-derived microbial consortium

Author: Adèle Lazuka, Guillermina Hernandez-Raquet, Cécile Roland, Michael J. O’Donohue, Abdellatif Barakat, Fabienne Guillon, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), French National Agency for Energy and the Environment (ADEME), CEPIA department of INRA, PROBIOS project funded by the Carnot Institute 3BCAR, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 0301 basic medicine, Acidogenesis, Rumen, Environmental Engineering, carboxylate, bioconversion, Microbial Consortia, Anaerobic microbial consortium, Ingénierie des aliments, Bioengineering, 01 natural sciences, Lignocellulose bioconversion, 03 medical and health sciences, chemistry.chemical_compound, 010608 biotechnology, [SDV.IDA]Life Sciences [q-bio]/Food engineering, écoprocédé, Animals, Food engineering, Carboxylate, Food science, neoplasms, Waste Management and Disposal, Triticum, 2. Zero hunger, paille de blé, wheat straw, Renewable Energy, Sustainability and the Environment, Substrate (chemistry), food and beverages, General Medicine, Straw, Microbial consortium, pretreatment, Dry chemo-mechanical pretreatment, Enzymes, stomatognathic diseases, Carboxylate production, 030104 developmental biology, chemistry, Biochemistry, Xylanase, Composition (visual arts), prétraitement
Abstract: Innovative dry chemo- and chemo-mechanical pretreatments form an interesting approach for modifying the native physico-chemical composition of lignocellulose facilitating its microbial conversion to carboxylates. Here, the impact of four dry-pretreatment conditions on the microbial transformation of wheat straw was assessed: milling to 2 mm and 100 µm, and NaOH chemical impregnation at high substrate concentrations combined with milling at 2 mm and 100 µm. Pretreatment effect was assessed in the light of substrate structure and composition, its impact on the acidogenic potential and the major enzyme activities of a rumen-derived microbial consortium RWS. Chemo-mechanical pretreatment strongly modified the substrate macroporosity. The highest carboxylate production rate was reached after dry chemo-mechanical treatment with NaOH at 100 µm. A positive impact of the dry chemo-mechanical treatment on xylanase activity was observed also. These results underline that increasing substrate macroporosity by dry chemo-mechanical pretreatment had a positive impact on the microbial acidogenic potential.
Published: 2017
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16. CAZyChip: dynamic assessment of exploration of glycoside hydrolases in microbial ecosystems

Author: Michael J. O’Donohue, Lucas Auer, Elisabeth Laville, Bernard Henrissat, Claire Dumon, Vincent Lombard, Guillermina Hernandez-Raquet, Véronique Anton Leberre, Sophie Lamarre, Anne Abot, Adèle Lazuka, Grégory Arnal, Delphine Labourdette, Gabrielle Potocki-Véronèse, Lidwine Trouilh, Architecture et fonction des macromolécules biologiques ( AFMB ), Centre National de la Recherche Scientifique ( CNRS ) -Aix Marseille Université ( AMU ) -Institut National de la Recherche Agronomique ( INRA ), Conseil Régional Midi-Pyrénées 12053333, IDEX Univ. Toulouse 2014–628, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Architecture et fonction des macromolécules biologiques (AFMB), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), King Abdulaziz University, Leberre, Véronique, Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Glycan, CAZy, Glycoside Hydrolases, [SDV]Life Sciences [q-bio], Computational biology, Microarray, Microbial functional diversity, Proteomics, medicine.disease_cause, Transcriptomic analysis, 03 medical and health sciences, Bacterial Proteins, Cell Wall, Polysaccharides, Databases, Genetic, medicine, Genetics, Escherichia coli, Glycoside hydrolase, Bioprocess, [ SDV.BIBS ] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], ComputingMilieux_MISCELLANEOUS, Oligonucleotide Array Sequence Analysis, biology, business.industry, Sequence Analysis, RNA, Gene Expression Regulation, Bacterial, Plants, CAZymes detection, Biotechnology, Plant cell wall degradation, 030104 developmental biology, Metagenomics, biology.protein, Metagenome, DNA microarray, business, hormones, hormone substitutes, and hormone antagonists, Research Article, [ SDV.BBM.BS ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract: Background Microorganisms constitute a reservoir of enzymes involved in environmental carbon cycling and degradation of plant polysaccharides through their production of a vast variety of Glycoside Hydrolases (GH). The CAZyChip was developed to allow a rapid characterization at transcriptomic level of these GHs and to identify enzymes acting on hydrolysis of polysaccharides or glycans. Results This DNA biochip contains the signature of 55,220 bacterial GHs available in the CAZy database. Probes were designed using two softwares, and microarrays were directly synthesized using the in situ ink-jet technology. CAZyChip specificity and reproducibility was validated by hybridization of known GHs RNA extracted from recombinant E. coli strains, which were previously identified by a functional metagenomic approach. The GHs arsenal was also studied in bioprocess conditions using rumen derived microbiota. Conclusions The CAZyChip appears to be a user friendly tool for profiling the expression of a large variety of GHs. It can be used to study temporal variations of functional diversity, thereby facilitating the identification of new efficient candidates for enzymatic conversions from various ecosystems. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-2988-4) contains supplementary material, which is available to authorized users.
Published: 2016
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17. Occurrence, fate, and biodegradation of estrogens in sewage and manure

Author: Guillermina Hernandez-Raquet, Sarah Combalbert, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)
Subjects: Estrone, TREATMENT PROCESSES, Sewage, BIODEGRADATION, Endocrine Disruptors, 010501 environmental sciences, Biology, Ethinyl Estradiol, 01 natural sciences, Applied Microbiology and Biotechnology, Feces, 03 medical and health sciences, chemistry.chemical_compound, Estradiol Congeners, ENDOCRINE DISRUPTION, Animals, Humans, Microbial biodegradation, 030304 developmental biology, 0105 earth and related environmental sciences, Pollutant, 0303 health sciences, Bacteria, Estradiol, Estriol, business.industry, Estrogens, General Medicine, Manure, 6. Clean water, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Biodegradation, Environmental, chemistry, 13. Climate action, Environmental chemistry, Sewage treatment, business, Biotechnology, Hormone
Abstract: The estrogens estrone (E1), 17 alpha-estradiol (E2 alpha), 17 beta-estradiol (E2 beta), and estriol (E3) are natural sex hormones produced by humans and animals. In addition, there are some synthetic estrogens, such as 17 alpha-ethinylestradiol (EE2), used for contraception purposes. These compounds are able to produce endocrine disruption in living organisms at nanogram-per-liter levels. In both humans and animals, estrogens are excreted in urine and feces, reaching the natural environment through discharge from sewage treatment plants (STP) and manure disposal units. In STPs, hormone removal depends on the type of treatment process and on different parameters such as the hydraulic and sludge retention times. Thus, hormone elimination rates vary from 0% to 90% in different STPs. Animals are also an important source of estrogens in the environment. Indeed, animals produce high concentrations of hormones which will end up in manure which is typically spread on land. Hence, waste-borne animal hormones may transfer these pollutants to the soil. The purpose of this review is to highlight the significance for both health and the environment of pollution by estrogens and critically review the existing knowledge on their fate and removal in different treatment processes. Relevant information on the microbial degradation of hormones and metabolic pathways is also included.
Published: 2010
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18. Development and validation of an analytical method by LC-MS/MS for the quantification of estrogens in sewage sludge

Author: Bernard Herbreteau, Virginie Gabet-Giraud, Guillermina Hernandez-Raquet, Cecile Miege, Marina Coquery, Milieux aquatiques, écologie et pollutions (UR MALY), Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF), Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)
Subjects: EAU USEE, Relative standard deviation, ESTROGENS, Analytical chemistry, 010501 environmental sciences, Mass spectrometry, 01 natural sciences, Biochemistry, Analytical Chemistry, METHOD VALIDATION, Tandem Mass Spectrometry, WASTEWATER TREATMENT PLANT, Lc ms ms, Chromatography, High Pressure Liquid, 0105 earth and related environmental sciences, Reproducibility, Chromatography, Sewage, Chemistry, Solid Phase Extraction, SEWAGE SLUDGE, 010401 analytical chemistry, Extraction (chemistry), Repeatability, 6. Clean water, LC-MSMS ANALYSIS, 0104 chemical sciences, CHIMIE ANALYTIQUE, [SDE]Environmental Sciences, MATRIX EFFECTS, Sludge
Abstract: International audience; A sensitive method for the simultaneous analysis of five estrogens in sewage sludge was developed. The extraction and purification steps were optimized and the matrix effects were evaluated. The chromatographic gradient was optimized to limit matrix effects and the analysis step was performed by LC-MS/MS. The method consists of an ASE® extraction with a solvent mixture water/methanol 80/20v/v at 100°C followed by two consecutive purifications on Oasis HLB® and florisil cartridges. A thorough validation of the developed method was performed. Recoveries determined at two different spiking levels ranged between 86% and 126% depending on the molecule. Repeatability was evaluated on five replicates of the same sludge sample spiked at two different levels and measuring native estrogens in triplicates of 12 sludge samples. Relative standard deviations obtained a range of between 2% and 27%. Reproducibility was also studied by analyzing the same sludge on four different days: the relative standard deviation ranged between 14% and 20% for E1, βE2 and E3. For αE2, poor reproducibility (68%) was observed but it was linked to the very low quantity of αE2 present in the sludge sample and not to the method performance. The specificity of the method was evaluated on various sludge samples spiked at different spiking levels showing that performances of the proposed method were not modified by matrix effects. Finally, sensitivity of the method was evaluated taking into account both instrumental sensitivity and matrices; the estimated limits of quantification were around 1 ng/g for E1, between 2 and 4 ng/g for αE2, βE2, and E3 and around 5 ng/g for EE2.
Published: 2010
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19. Molecular diversity studies of bacterial communities of oil polluted microbial mats from the Etang de Berre (France)

Author: Hélène Budzinski, Robert Duran, Pierre Caumette, Patrick Dabert, Gerard Muyzer, Guillermina Hernandez-Raquet, and Karyn Le Menach
Subjects: 0303 health sciences, Ecology, 030306 microbiology, Ribosomal Intergenic Spacer analysis, 15. Life on land, Biology, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, Amplified Ribosomal DNA Restriction Analysis, Rhodobacterales, 03 medical and health sciences, 14. Life underwater, Species richness, Microbial mat, Proteobacteria, Ribosomal DNA, Temperature gradient gel electrophoresis, 030304 developmental biology
Abstract: The biodiversity of microbial mats inhabiting the oil-contaminated lagoon Etang de Berre was determined by molecular approaches. The fingerprint of denaturing gradient gel electrophoresis (DGGE) and automatic ribosomal intergenic spacer analysis (ARISA) of mats exposed to different pollution levels showed specific microbial communities for each site but similar diversity richness. Species composition of the mats were compared by constructing 16S rRNA libraries. Amplified rDNA restriction analysis (ARDRA) of clone libraries confirmed their similar level of diversity richness. Phylogenetic analysis of the 16S rRNA sequences showed that the classes γ and α of Proteobacteria were abundantly present in both sites whereas phylotypes related to the δ-Proteobacteria and to the uncultured WS3 group were mainly found in the site with the highest pollution. Identification of the species involved in oil degradation by combining culture-based approaches and DGGE, showed that enrichment cultures were constituted by members of the Rhodobacterales and species related to Rhodococcus , Sphingomonas , Xanthomonas and Microbacterium , all of them known for their ability to degrade hydrocarbons. Our findings suggest that oil pollution has not affected the biodiversity richness of the mats. However, the populations involved in hydrocarbon degradation represent a minor fraction of the mat communities in the Etang de Berre .
Published: 2006
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20. Efficient anaerobic transformation of raw wheat straw by a robust cow rumen-derived microbial consortium

Author: Diego P. Morgavi, Michael J. O’Donohue, Lucas Auer, Adèle Lazuka, Guillermina Hernandez-Raquet, Sophie Bozonnet, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, French National Agency for Energy and Environment (ADEME), Carnot Institute 3BCAR, INRA Metascreen project, Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés ( LISBP ), Institut National de la Recherche Agronomique ( INRA ) -Institut National des Sciences Appliquées - Toulouse ( INSA Toulouse ), Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), and VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA )
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, bioconversion, Bioconversion, Biomass, 7. Clean energy, Lignin, lignocellulose, Bioreactors, Food science, Anaerobiosis, Waste Management and Disposal, fermentation, Triticum, 2. Zero hunger, paille de blé, cellulase, biology, Chemistry, Hydrolysis, food and beverages, General Medicine, Microbial consortium, Straw, Biofuel, Female, Mesophile, anaerobic consortium, Environmental Engineering, Rumen, animal structures, carboxylate, Firmicutes, Microbial Consortia, Lignocellulosic biomass, lignocellulose bioconversion, carboxylate production, xylanase, Bioengineering, Biotechnologies, Animals, dégradation des fibres, Renewable Energy, Sustainability and the Environment, [ SDV.BIO ] Life Sciences [q-bio]/Biotechnology, biology.organism_classification, procédé anaérobie, Fatty Acids, Volatile, Agronomy, Biofuels, Cattle
Abstract: A rumen-derived microbial consortium was enriched on raw wheat straw as sole carbon source in a sequential batch-reactor (SBR) process under strict mesophilic anaerobic conditions. After five cycles of enrichment the procedure enabled to select a stable and efficient lignocellulolytic microbial consortium, mainly constituted by members of Firmicutes and Bacteroidetes phyla. The enriched community, designed rumen-wheat straw-derived consortium (RWS) efficiently hydrolyzed lignocellulosic biomass, degrading 55.5% w/w of raw wheat straw over 15 days at 35 degrees C and accumulating carboxylates as main products. Cellulolytic and hemicellulolytic activities, mainly detected on the cell bound fraction, were produced in the earlier steps of degradation, their production being correlated with the maximal lignocellulose degradation rates. Overall, these results demonstrate the potential of RWS to convert unpretreated lignocellulosic substrates into useful chemicals.
Published: 2015
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21. Role of environmental fluctuations and microbial diversity in degradation of hydrocarbons in contaminated sludge

Author: Virginie Bellet, Guillermina Hernandez-Raquet, Ronan Jézéquel, Patrick Balaguer, Robert Duran, Cristiana Cravo-Laureau, Isabelle Vitte, Jean-Jacques Godon, Pierre Caumette, Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Univers (INSU), and Communauté d'Agglomération de Pau Pyrénées
Subjects: Microbial diversity, Microbial Consortia, oxic/anoxic oscillation, 010501 environmental sciences, Biology, 01 natural sciences, Microbiology, Redox, diversity, 03 medical and health sciences, Soil Pollutants, Ecosystem, Anaerobiosis, Polycyclic Aromatic Hydrocarbons, Molecular Biology, Soil Microbiology, 030304 developmental biology, 0105 earth and related environmental sciences, degradation, Pollutant, chemistry.chemical_classification, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, 0303 health sciences, Sewage, Ecology, Biodiversity, General Medicine, PAH, Contamination, Anoxic waters, Aerobiosis, Biodegradation, Environmental, Hydrocarbon, chemistry, 13. Climate action, Environmental chemistry, ecosystem functioning, Degradation (geology), human activities
Abstract: cited By 12; International audience; Little is known about microbial communities involved in hydrocarbon degradation, whether it be their structural and functional diversity or their response to environmental constraints such as oxygen fluctuation. Here, current knowledge of the impact of diversity and redox oscillations upon ecosystem processes is reviewed. In addition, we present the main conclusions of our studies in this field. Oxic/anoxic oscillations had a strong impact upon bacterial community structures, influencing their ability to degrade hydrocarbons and their capacity to reduce hydrocarbon toxicity. Furthermore, a decrease in functional diversity has a strong impact on pollutant degradation.
Published: 2011
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22. Molecular and kinetic characterization of mixed cultures degrading natural and synthetic estrogens

Author: Guillermina Hernandez-Raquet, Mathieu Muller, Jean-Philippe Delgenès, Jean-Jacques Godon, Dominique Patureau, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)
Subjects: DNA, Bacterial, Molecular Sequence Data, Estrone, 010501 environmental sciences, DNA, Ribosomal, 01 natural sciences, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Biotransformation, RNA, Ribosomal, 16S, Cluster Analysis, Brevundimonas diminuta, BREVUNDIMONAS, ETHINYLESTRADIOL, ESTRADIOL, Phylogeny, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, Chromatography, Alcaligenes faecalis, Bacteria, Sewage, biology, Brevundimonas, Estrogens, Sequence Analysis, DNA, General Medicine, Biodegradation, DEGRADATION, biology.organism_classification, Kinetics, ESTROGEN, Activated sludge, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, chemistry, ESTRONE, Biotechnology
Abstract: The biodegradation of estradiol (E2), estrone (E1), and ethinylestradiol (EE2) was investigated using mixed bacterial cultures enriched from activated sludge. Enrichments were carried out on E2 or EE2 in batch conditions with acetonitrile as additional carbon source. Degradation experiments were performed both using hormones as sole carbon source or with an additional source. The hormones were completely degraded by these cultures. Estradiol was rapidly converted to E1 within 24 h. Thereafter, E1 degradation began, displaying a lag phase ranging from 3 to 4 days. Estrone depletion took from 48 h to more than 6 days, depending on the culture conditions. For EE2 degradation, when it was the sole carbon source, the lag phase and the time required for its complete removal (7 and 15 days, respectively) were shorter that in cultures with a supplementary carbon source. The specific degradation rates observed for E2 both with and without an additional carbon source were similar. By contrast, the specific degradation rates for E1 and EE2 were, respectively, seven and 20 times faster when these hormones were supplied as the sole carbon source. The bacterial community structure of each culture was characterized by molecular and cultural methods. The mixed cultures were made up of species belonging to Alcaligenes faecalis, Pusillimonas sp., Denitrobacter sp., and Brevundimonas diminuta or related to uncultured Bacteroidetes. The isolated strain B. diminuta achieved the conversion of E2 to E1.
Published: 2010
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23. Occurrence of estrogens in sewage sludge and their fate during plant-scale anaerobic digestion

Author: Dominique Patureau, Nadine Delgenès, Vincent Rocher, Jean-Philippe Delgenès, Pierre Benoit, Sarah Combalbert, Guillermina Hernandez-Raquet, Mathieu Muller, Valérie Bergheaud, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), Environnement et Grandes Cultures (EGC), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Direction du Développement et de la Prospective, Syndicat Interdépartemental pour l'Assainissement de l'Agglomération Parisienne, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech
Subjects: Secondary treatment, Environmental Engineering, Estrone, Health, Toxicology and Mutagenesis, [SDE.MCG]Environmental Sciences/Global Changes, VALIDATION PROCEDURE, 0207 environmental engineering, ESTROGENS, ANAEROBIC DIGESTION, Sewage, 02 engineering and technology, 010501 environmental sciences, Ethinyl Estradiol, SORPTION, Waste Disposal, Fluid, 01 natural sciences, ANALYTICAL METHOD, Environmental Chemistry, Anaerobiosis, 020701 environmental engineering, 0105 earth and related environmental sciences, SLUDGE, Chromatography, Estradiol, Estriol, business.industry, Chemistry, Public Health, Environmental and Occupational Health, Sorption, General Medicine, General Chemistry, Pollution, 6. Clean water, Waste treatment, Anaerobic digestion, Wastewater, Environmental chemistry, Sewage treatment, business, Water Pollutants, Chemical, Sludge, Environmental Monitoring
Abstract: Estrogens, which contribute greatly to the endocrine-disrupting activity in sewage, are partially sorbed onto particulate matter during sewage treatment. We thus investigated the occurrence of estrogens in different kinds of sludge and throughout a plant-scale anaerobic digestion process. The analytical method was first validated when sorption interaction between spiked estrogens and sludge could occur. Hence, the recovery ratio of estrone (E1), 17[beta]-estradiol (E2), estriol (E3) and 17[alpha]-ethinylestradiol (EE2) were determined when added to liquid sludge and mixed under various conditions. We show that minor non-extractable residues were formed (5-10%), suggesting that the sorption interaction established with sludge did not limit estrogen extraction. Estrogen concentrations measured in collected samples varied with sludge type. Secondary sludge showed higher E1 contents than primary sludge: respectively, 43 and 8 ng g-1 dry weight (dw). Two pathways of E1 production during secondary treatment are proposed to explain such a result. Higher estrogen concentrations were found in secondary sludge from a conventional plant (55 ng g-1 dw) compared to those from an advanced plant (13 ng g-1 dw). Based on estimated estrogen concentrations in sewage, we conclude that operating parameters play a role in the sorption of estrogens during secondary treatment. Also, the hydrophobic properties of the estrogens influenced the individual adsorption of each molecule. Thus, E3 showed the highest estimated concentrations in sewage but very low concentrations in sludge. Finally, plant-scale anaerobic digestion showed low efficiency (
Published: 2010
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24. Enhanced methods for conditioning, storage, and extraction of liquid and solid samples of manure for determination of steroid hormones by solid-phase extraction and gas chromatography-mass spectrometry

Author: Guillermina Hernandez-Raquet, Sarah Combalbert, Marie-Laure Pype, Nicolas Bernet, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)
Subjects: MASS SPECTROMETRY, Swine, [SDV]Life Sciences [q-bio], STEROID HORMONES ANALYSIS, 010501 environmental sciences, 01 natural sciences, Biochemistry, Gas Chromatography-Mass Spectrometry, Analytical Chemistry, ENDOCRINE DISRUPTORS, AGRICULTURAL WASTES, Liquid–liquid extraction, Animals, Solid phase extraction, GAS CHROMATROGRAPHY, HORMONE STEROIDE, SWINE MANURE, 0105 earth and related environmental sciences, Chromatography, Chemistry, 010401 analytical chemistry, Extraction (chemistry), Solid Phase Extraction, Manure, 6. Clean water, Hormones, 0104 chemical sciences, Wastewater, Environmental chemistry, [SDE]Environmental Sciences, Steroids, Gas chromatography, Gas chromatography–mass spectrometry, Sludge
Abstract: Hormones are among the highest-impact endocrine disrupters affecting living organisms in aquatic environments. These molecules have been measured in both wastewater and sewage sludge. Analytical techniques for such matrices are well described in the literature. In contrast, there is little information about the analysis of hormones in animal waste. The objectives of this study were, first, to propose a method for conditioning swine manure samples (addition of formaldehyde, separation of the solid and liquid phases, and duration of storage) in order to determine hormones in the liquid fraction of manure by solid-phase extraction (SPE) coupled with gas chromatography-mass spectrometry (GC-MS). Our results showed that analysis of hormones was affected by matrix changes which occurred during freezing and thawing and after addition of formaldehyde, an additive frequently used to preserve environmental samples. Thus, our results argue for the conditioning of samples without formaldehyde and for separating the solid and liquid fractions of manure before freezing. Second, this study reports on the use of a liquid extraction method coupled with SPE and GC-MS analysis for determination of hormones in the solid fraction of manure. Under the conditions selected, hormone recoveries were between 80 and 100%. Finally, the optimized method was used to quantify hormones in both liquid and solid fractions of swine manure from different breeding units. High levels of estrone and α-estradiol were found in samples whereas β-estradiol was detected in smaller amounts. Estriol and progesterone were mainly found in manure from the gestating sow building whereas testosterone was detected in manure from male breeding buildings.
Published: 2010
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25. Community structure and seasonal dynamics of diatom biofilms and associated grazers in intertidal mudflats

Author: Guillermina Hernandez-Raquet, Véronique Créach, Emel Sahan, Koen Sabbe, Gerard Muyzer, Wim Vyverman, Lucas J. Stal, Department of Biotechnology, Delft University of Technology (TU Delft), Universiteit Gent = Ghent University [Belgium] (UGENT), Royal Netherlands Academy of Arts and Sciences (KNAW), Centre for Environment, Fisheries and Aquaculture Science (CEFAS), Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Aquatic Microbiology (IBED, FNWI), Marine Microbiology, and ProdInra, Migration
Subjects: 0106 biological sciences, Chlorophyll a, Population dynamics, Intertidal zone, [SDV.SA.STP] Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery, WESTERSCHELDE ESTUARY, Aquatic Science, 010603 evolutionary biology, 01 natural sciences, salinity gradient, chemistry.chemical_compound, seasonal dynamics, Tidal flats, eukaryotes, Bacillariophyceae [Featherlike diatoms], 14. Life underwater, Ecology, Evolution, Behavior and Systematics, Diatoms, ANE, Netherlands, Westerschelde, PaulinaSchor, PCR-DGGE, Biomass (ecology), geography, Mud flats, geography.geographical_feature_category, Brackish water, biology, ANE, Netherlands, Westerschelde, Appelzak, Ecology, 010604 marine biology & hydrobiology, fungi, Community structure, Estuary, BIOLOGIE MOLECULAIRE, biology.organism_classification, Grazing, Diatom, chemistry, Navicula, Salinity gradients, ANE, Netherlands, Westerschelde, Biezelingse Ham Mud Flat, Biofilms, [SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery, intertidal mudflat, epipelic diatoms
Abstract: International audience; The composition and seasonal dynamics of biofilm-associated eukaryotic communities were analysed at the metre and kilometre scale along a salinity gradient in the Westerschelde estuary (The Netherlands), using microscopy and a genetic fingerprinting technique (PCR-DGGE). Microphytobenthic biomass, measured as chlorophyll a (chl a), varied seasonally over 2 orders of magnitude, being highest in spring. Communities were dominated by epipelic diatoms, in particular by members of the genus Navicula. In spring, a few smaller epipelic diatom species dominated during biomass peaks, while during the rest of the year, communities were more diverse and were characterised by larger species. The microphytobenthic community collapsed when grazers appeared, which happened concomitantly with a rise in temperature. Spring biomass development was associated with marked changes in porewater nutrient concentrations, especially towards the estuary mouth. In the DGGE data, diatoms, ciliates, amoebae, copepods, nematodes, annelids and platyhelminthes were detected. Ordination analysis of the species counts and DGGE data were largely congruent and indicated that on the scale of the whole estuary (i.e. km scale), taxonomic turnover in microphytobenthos composition was mainly associated with the salinity gradient. At smaller spatial scales, the position of sampling localities along the tidal exposure gradient appeared to be the main determinant of species turnover, in particular in the brackish reaches of the estuary.
Published: 2007

26. Molecular diversity studies of bacterial communities of oil polluted microbial mats from the Etang de Berre (France)

Author: Guillermina, Hernandez-Raquet, Hélène, Budzinski, Pierre, Caumette, Patrick, Dabert, Karyn, Le Ménach, Gerard, Muyzer, and Robert, Duran
Subjects: DNA, Bacterial, Bacteria, DNA, Ribosomal Spacer, Mediterranean Sea, Electrophoresis, Polyacrylamide Gel, Fresh Water, Biodiversity, Genome, Bacterial, Hydrocarbons, Phylogeny, Water Pollutants, Chemical
Abstract: The biodiversity of microbial mats inhabiting the oil-contaminated lagoon Etang de Berre was determined by molecular approaches. The fingerprint of denaturing gradient gel electrophoresis (DGGE) and automatic ribosomal intergenic spacer analysis (ARISA) of mats exposed to different pollution levels showed specific microbial communities for each site but similar diversity richness. Species composition of the mats were compared by constructing 16S rRNA libraries. Amplified rDNA restriction analysis (ARDRA) of clone libraries confirmed their similar level of diversity richness. Phylogenetic analysis of the 16S rRNA sequences showed that the classes gamma and alpha of Proteobacteria were abundantly present in both sites whereas phylotypes related to the delta-Proteobacteria and to the uncultured WS3 group were mainly found in the site with the highest pollution. Identification of the species involved in oil degradation by combining culture-based approaches and DGGE, showed that enrichment cultures were constituted by members of the Rhodobacterales and species related to Rhodococcus, Sphingomonas, Xanthomonas and Microbacterium, all of them known for their ability to degrade hydrocarbons. Our findings suggest that oil pollution has not affected the biodiversity richness of the mats. However, the populations involved in hydrocarbon degradation represent a minor fraction of the mat communities in the Etang de Berre.
Published: 2006

27. MOESM2 of Anaerobic lignocellulolytic microbial consortium derived from termite gut: enrichment, lignocellulose degradation and community dynamics

Author: Lazuka, Adèle, Auer, Lucas, O’Donohue, Michael, and Guillermina Hernandez-Raquet
Subjects: 15. Life on land
Abstract: Additional file 2. Sparse PLS-DA applied to degradation peak and degradation plateau points.

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27 results on '"Guillermina Hernandez-Raquet"'

1. Anaerobic lignocellulolytic microbial consortium derived from termite gut: enrichment, lignocellulose degradation and community dynamics

2. Screening of Phytophagous and Xylophagous Insects Guts Microbiota Abilities to Degrade Lignocellulose in Bioreactor

3. Uncovering the Potential of Termite Gut Microbiome for Lignocellulose Bioconversion in Anaerobic Batch Bioreactors

4. Impact of phosphorus limitation on medium-chain-length polyhydroxyalkanoate production by activated sludge

5. Termite Gut Microbiota Contribution to Wheat Straw Delignification in Anaerobic Bioreactors

6. Enrichment and adaptation yield high anammox conversion rates under low temperatures

7. Avis en réponse à la saisine HCB - dossier EFSA-GMO-RX-013. Paris, le 30 janvier 2019

8. Avis en réponse à la saisine HCB - EFSA-GMO-ES-2018-154. Paris, le 5 avril 2019

9. Avis en réponse à la saisine HCB - dossier RX-015. Paris, le 26 février 2019

10. Avis en réponse à la saisine HCB - dossier 2018-150. Paris, le 24 octobre 2018

11. Avis en réponse à la saisine HCB - dossier 2017-143. Paris, le 15 mai 2018

12. Screening of Phytophagous and Xylophagous insects guts microbiota abilities to degrade lignocellulose in bioreactor

13. Anaerobic lignocellulolytic microbial consortium derived from termite gut: enrichment, lignocellulose degradation and community dynamics

14. FROGS: Find, Rapidly, OTUs with Galaxy Solution

15. Ecofriendly lignocellulose pretreatment to enhance the carboxylate production of a rumen-derived microbial consortium

16. CAZyChip: dynamic assessment of exploration of glycoside hydrolases in microbial ecosystems

17. Occurrence, fate, and biodegradation of estrogens in sewage and manure

18. Development and validation of an analytical method by LC-MS/MS for the quantification of estrogens in sewage sludge

19. Molecular diversity studies of bacterial communities of oil polluted microbial mats from the Etang de Berre (France)

20. Efficient anaerobic transformation of raw wheat straw by a robust cow rumen-derived microbial consortium

21. Role of environmental fluctuations and microbial diversity in degradation of hydrocarbons in contaminated sludge

22. Molecular and kinetic characterization of mixed cultures degrading natural and synthetic estrogens

23. Occurrence of estrogens in sewage sludge and their fate during plant-scale anaerobic digestion

24. Enhanced methods for conditioning, storage, and extraction of liquid and solid samples of manure for determination of steroid hormones by solid-phase extraction and gas chromatography-mass spectrometry

25. Community structure and seasonal dynamics of diatom biofilms and associated grazers in intertidal mudflats

26. Molecular diversity studies of bacterial communities of oil polluted microbial mats from the Etang de Berre (France)

27. MOESM2 of Anaerobic lignocellulolytic microbial consortium derived from termite gut: enrichment, lignocellulose degradation and community dynamics

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27 results on '"Guillermina Hernandez-Raquet"'

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