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2. Exercise Suppresses Head and Neck Squamous Cell Carcinoma Growth via Oncostatin M

Author

Yoshimura, Takuya, primary, Hirano, Yuka, additional, Hamada, Taiji, additional, Yokoyama, Seiya, additional, Suzuki, Hajime, additional, Takayama, Hirotaka, additional, Migita, Hirono, additional, Ishida, Takayuki, additional, Nakamura, Yasunori, additional, Ohsawa, Masahiro, additional, Asakawa, Akihiro, additional, Ishihata, Kiyohide, additional, and Tanimoto, Akihide, additional

Published
2024
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6. Alterations in EGFR and PDGFRA are Associated with the Localization of Contrast-Enhancing Lesions in Glioblastoma

Author

Makino, Ryutaro, primary, Higa, Nayuta, additional, Akahane, Toshiaki, additional, Yonezawa, Hajime, additional, Uchida, Hiroyuki, additional, Takajo, Tomoko, additional, Fujio, Shingo, additional, Kirishima, Mari, additional, Hamada, Taiji, additional, Yamahata, Hitoshi, additional, Kamimura, Kiyohisa, additional, Yoshiura, Takashi, additional, Yoshimoto, Koji, additional, Tanimoto, Akihide, additional, and Hanaya, Ryosuke, additional

Published
2023
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7. Favorable prognostic impact of phosphatase and tensin homolog alterations in wild-type isocitrate dehydrogenase and telomerase reverse transcriptase promoter glioblastoma

Author

Higa, Nayuta, primary, Akahane, Toshiaki, additional, Yokoyama, Seiya, additional, Makino, Ryutaro, additional, Yonezawa, Hajime, additional, Uchida, Hiroyuki, additional, Takajo, Tomoko, additional, Kirishima, Mari, additional, Hamada, Taiji, additional, Noguchi, Naoki, additional, Otsuji, Ryosuke, additional, Kuga, Daisuke, additional, Nagasaka, Shohei, additional, Yamahata, Hitoshi, additional, Yamamoto, Junkoh, additional, Yoshimoto, Koji, additional, Tanimoto, Akihide, additional, and Hanaya, Ryosuke, additional

Published
2023
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9. Distribution and favorable prognostic implication of genomicEGFRalterations inIDH‐wildtype glioblastoma

Author

Higa, Nayuta, primary, Akahane, Toshiaki, additional, Hamada, Taiji, additional, Yonezawa, Hajime, additional, Uchida, Hiroyuki, additional, Makino, Ryutaro, additional, Watanabe, Shoji, additional, Takajo, Tomoko, additional, Yokoyama, Seiya, additional, Kirishima, Mari, additional, Matsuo, Kei, additional, Fujio, Shingo, additional, Hanaya, Ryosuke, additional, Tanimoto, Akihide, additional, and Yoshimoto, Koji, additional

Published
2022
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10. Genome Editing Using Cas9 Ribonucleoprotein Is Effective for Introducing PDGFRA Variant in Cultured Human Glioblastoma Cell Lines.

Author

Hamada, Taiji, Yokoyama, Seiya, Akahane, Toshiaki, Matsuo, Kei, and Tanimoto, Akihide

Subjects
*GENOME editing, *CRISPRS, *CELL lines, *PLASMIDS, *PLATELET-derived growth factor receptors, *CANCER cell analysis
Abstract

Many variants of uncertain significance (VUS) have been detected in clinical cancer cases using next-generation sequencing-based cancer gene panel analysis. One strategy for the elucidation of VUS is the functional analysis of cultured cancer cell lines that harbor targeted gene variants using genome editing. Genome editing is a powerful tool for creating desired gene alterations in cultured cancer cell lines. However, the efficiency of genome editing varies substantially among cell lines of interest. We performed comparative studies to determine the optimal editing conditions for the introduction of platelet-derived growth factor receptor alpha (PDGFRA) variants in human glioblastoma multiforme (GBM) cell lines. After monitoring the copy numbers of PDGFRA and the expression level of the PDGFRα protein, four GBM cell lines (U-251 MG, KNS-42, SF126, and YKG-1 cells) were selected for the study. To compare the editing efficiency in these GBM cell lines, the modes of clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (Cas9) delivery (plasmid vs. ribonucleoprotein (RNP)), methods of transfection (lipofection vs. electroporation), and usefulness of cell sorting were then evaluated. Herein, we demonstrated that electroporation-mediated transfer of Cas9 with single-guide RNA (Cas9 RNP complex) could sufficiently edit a target nucleotide substitution, irrespective of cell sorting. As the Cas9 RNP complex method showed a higher editing efficiency than the Cas9 plasmid lipofection method, it was the optimal method for single-nucleotide editing in human GBM cell lines under our experimental conditions. [ABSTRACT FROM AUTHOR]

Published
2023
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11. Distribution and favorable prognostic implication of genomic EGFR alterations in IDH‐wildtype glioblastoma.

Author

Higa, Nayuta, Akahane, Toshiaki, Hamada, Taiji, Yonezawa, Hajime, Uchida, Hiroyuki, Makino, Ryutaro, Watanabe, Shoji, Takajo, Tomoko, Yokoyama, Seiya, Kirishima, Mari, Matsuo, Kei, Fujio, Shingo, Hanaya, Ryosuke, Tanimoto, Akihide, and Yoshimoto, Koji

Subjects
EPIDERMAL growth factor receptors, PROGNOSIS, ISOCITRATE dehydrogenase, GLIOBLASTOMA multiforme
Abstract

Background: We aimed to evaluate the mutation profile, transcriptional variants, and prognostic impact of the epidermal growth factor receptor (EGFR) gene in isocitrate dehydrogenase (IDH)‐wildtype glioblastomas (GBMs). Methods: We sequenced EGFR, evaluated the EGFR splicing profile using a next‐generation sequencing oncopanel, and analyzed the outcomes in 138 grade IV IDH‐wildtype GBM cases. Results: EGFR mutations were observed in 10% of GBMs. A total of 23.9% of the GBMs showed EGFR amplification. Moreover, 25% of the EGFR mutations occurred in the kinase domain. Notably, EGFR alterations were a predictor of good prognosis (p = 0.035). GBM with EGFR alterations was associated with higher Karnofsky Performance Scale scores (p = 0.014) and lower Ki‐67 scores (p = 0.005) than GBM without EGFR alterations. EGFRvIII positivity was detected in 21% of EGFR‐amplified GBMs. We identified two other EGFR variants in GBM cases with deletions of exons 6–7 (Δe 6–7) and exons 2–14 (Δe 2–14). In one case, the initial EGFRvIII mutation transformed into an EGFR Δe 2–14 mutation during recurrence. Conclusions: We found that the EGFR gene profiles of GBM differ among cohorts and that EGFR alterations are good prognostic markers of overall survival in patients with IDH‐wildtype GBM. Additionally, we identified rare EGFR variants with longitudinal and temporal transformations of EGFRvIII. [ABSTRACT FROM AUTHOR]

Published
2023
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12. Prognostic impact of PDGFRA gain/amplification and MGMT promoter methylation status in patients with IDH wild-type glioblastoma

Author

Higa, Nayuta, primary, Akahane, Toshiaki, additional, Yokoyama, Seiya, additional, Yonezawa, Hajime, additional, Uchida, Hiroyuki, additional, Takajo, Tomoko, additional, Otsuji, Ryosuke, additional, Hamada, Taiji, additional, Matsuo, Kei, additional, Kirishima, Mari, additional, Hata, Nobuhiro, additional, Hanaya, Ryosuke, additional, Tanimoto, Akihide, additional, and Yoshimoto, Koji, additional

Published
2022
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13. A tailored next‐generation sequencing panel identified distinct subtypes of wildtype IDH and TERT promoter glioblastomas

Author

Higa, Nayuta, primary, Akahane, Toshiaki, additional, Yokoyama, Seiya, additional, Yonezawa, Hajime, additional, Uchida, Hiroyuki, additional, Takajo, Tomoko, additional, Kirishima, Mari, additional, Hamada, Taiji, additional, Matsuo, Kei, additional, Fujio, Shingo, additional, Hanada, Tomoko, additional, Hosoyama, Hiroshi, additional, Yonenaga, Masanori, additional, Sakamoto, Akihisa, additional, Hiraki, Tsubasa, additional, Tanimoto, Akihide, additional, and Yoshimoto, Koji, additional

Published
2020
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14. Predicted Prognosis of Patients with Pancreatic Cancer by Machine Learning

Author

Yokoyama, Seiya, primary, Hamada, Taiji, additional, Higashi, Michiyo, additional, Matsuo, Kei, additional, Maemura, Kosei, additional, Kurahara, Hiroshi, additional, Horinouchi, Michiko, additional, Hiraki, Tsubasa, additional, Sugimoto, Tomoyuki, additional, Akahane, Toshiaki, additional, Yonezawa, Suguru, additional, Kornmann, Marko, additional, Batra, Surinder K., additional, Hollingsworth, Michael A., additional, and Tanimoto, Akihide, additional

Published
2020
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19. TET1-mediated DNA hypomethylation regulates the expression of MUC4 in lung cancer

Author

Yokoyama, Seiya, primary, Higashi, Michiyo, additional, Tsutsumida, Hideaki, additional, Wakimoto, Jouji, additional, Hamada, Tomofumi, additional, Wiest, Edwin, additional, Matsuo, Kei, additional, Kitazono, Ikumi, additional, Goto, Yuko, additional, Guo, Xin, additional, Hamada, Taiji, additional, Yamada, Sohsuke, additional, Hiraki, Tsubasa, additional, Yonezawa, Suguru, additional, Batra, Surinder K., additional, Hollingsworth, Michael A., additional, and Tanimoto, Akihide, additional

Published
2017
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