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15. Inhalation of Atmospheric-Pressure Gas Plasma Attenuates Brain Infarction in Rats With Experimental Ischemic Stroke

Author

Chen, Ye, Yang, Bingyan, Xu, Lixin, Shi, Zhongfang, Han, Ruoyu, Yuan, Fang, Ouyang, Jiting, Yan, Xu, Ostrikov, Kostya Ken, Chen, Ye, Yang, Bingyan, Xu, Lixin, Shi, Zhongfang, Han, Ruoyu, Yuan, Fang, Ouyang, Jiting, Yan, Xu, and Ostrikov, Kostya Ken

Abstract

Previous studies suggest the potential efficacy of neuroprotective effects of gaseous atmospheric-pressure plasma (APP) treatment on neuronal cells. However, it remains unclear if the neuroprotective properties of the gas plasmas benefit the ischemic stroke treatment, and how to use the plasmas in the in vivo ischemic stroke models. Rats were subjected to 90 min middle cerebral artery occlusion (MCAO) to establish the ischemic stroke model and then intermittently inhaled the plasma for 2 min at 60 min MCAO. The regional cerebral blood flow (CBF) was monitored. Animal behavior scoring, magnetic resonance imaging (MRI), 2,3,5-triphenyltetrazolium chloride (TTC) staining, and hematoxylin and eosin (HE) staining were performed to evaluate the therapeutic efficacy of the gas plasma inhalation on MCAO rats. Intermittent gas plasma inhalation by rats with experimental ischemic stroke could improve neurological function, increase regional CBF, and decrease brain infarction. Further MRI tests showed that the gas plasma inhalation could limit the ischemic lesion progression, which was beneficial to improve the outcomes of the MCAO rats. Post-stroke treatment with intermittent gas plasma inhalation could reduce the ischemic lesion progression and decrease cerebral infarction volume, which might provide a new promising strategy for ischemic stroke treatment.

Published
2022

16. Mechanisms of atmospheric pressure plasma protection of neuronal cells under simulated ischemic stroke conditions

Author

Yan, Xu, Yang, Bingyan, Ouyang, Jiting, Zhang, Chenyang, Lai, Yu, Shi, Zhongfang, Han, Ruoyu, Zhang, Wei, Yuan, Fang, Ostrikov, Kostya, Yan, Xu, Yang, Bingyan, Ouyang, Jiting, Zhang, Chenyang, Lai, Yu, Shi, Zhongfang, Han, Ruoyu, Zhang, Wei, Yuan, Fang, and Ostrikov, Kostya

Abstract

Physico-chemical and biological effects of atmospheric pressure plasmas (APPs) find numerous applications in biotechnology, medicine, and other fields. Recent studies revealed APPs' potential for ischemic stroke treatment through the protection of neuronal cells from injuries. However, the mechanisms of the plasma neuroprotection effects still remain unknown. This study reveals the key mechanisms of APP plasma jet (APPJ) enabled reduction of neuronal cell death caused by oxygen and glucose deprivation (OGD) under stroke-relevant conditions. Plasma reduced OGD induced apoptosis of SH-SY5Y neuronal cells is based on reactive oxygen and nitrogen species production and on nitric oxide related activation of the cyclic guanosine monophosphate (cGMP) and cGMP-dependent protein kinase G (PKG) pathway, followed by the Bcl-2/Bax level modulation and caspase3/9 activity inhibition. In addition, the protective effect of APPJ treatment on OGD injured SH-SY5Y cells could be abolished by cGMP pathway inhibitor LY83583 pretreatment. Collectively, our findings highlight that the mechanism of the neuroprotection effects of the plasma treatment is closely related to the intracellular cGMP/PKG pathway, which provide experimental and theoretical references for future studies on plasma medicine.

Published
2022

21. Atmospheric pressure plasma treatments protect neural cells from ischemic stroke-relevant injuries by targeting mitochondria

Author

Yan, Xu, Zhang, Chenyang, Ouyang, Jiting, Shi, Zhongfang, Chen, Ye, Han, Ruoyu, Zhang, Wei, Yuan, Fang, Ostrikov, Kostya, Yan, Xu, Zhang, Chenyang, Ouyang, Jiting, Shi, Zhongfang, Chen, Ye, Han, Ruoyu, Zhang, Wei, Yuan, Fang, and Ostrikov, Kostya

Abstract

Most studies regarding plasma biomedicine applications mainly focus on the oxidative and/or nitrative stress on bacteria, cancer cells, and other treatment objects. In this study, we evaluate the protective effect of appropriate atmospheric pressure plasma jet (APPJ) treatments on oxygen and glucose deprivation (OGD)-induced neural cell apoptosis, which is a major pathological process during ischemic stroke, based on the physiological functions of NO. Results show that APPJ treatment reduces the OGD-induced apoptosis by weakening typical OGD injury consequences including loss of mitochondrial membrane potential, the release of cytochrome c from the mitochondria into the cytoplasm, lower antiapoptotic Bcl-2 expression, and upregulating the proapoptotic protein Bax. Furthermore, APPJ increased intracellular NO production, which is closely related to the cytoprotective effect of APPJ.

Published
2020

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