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Objective To explore the effect and preliminary mechanism of stathmin on skin fibroblast migration in the early inflammatory reaction of wound healing mimicked by TNF-α stimulation. Methods Human dermal fibroblast was stimulated by TNF-α to establish a cell model of inflammation at the early stage of wound healing. The effect of TNF-α on filopodium formation was observed by immunofluorescence assay, cell migration was detected by scratch wound healing, and stathmin expression level under TNF-α stimulation was measured by immunofluorescence assay and Western blotting. After cytochalasin B stimulation and small interfering RNA were used to interfere stathmin expression, respectively, immunofluorescence assay and scratch wound healing were performed again to observe the changes of filopodia and cell migration. Results TNF-α treatment promoted the formation of filopodia in human dermal fibroblasts, enhanced cell migration (P < 0.05) and increased stathmin expression (P < 0.05). Cytochalasin B treatment reduced the formation of filopodia and cell migration (P < 0.05); and interference of stathmin expression suppressed the formation of filopodia and reduced cell migration (P < 0.05). Conclusion In early inflammation, stathmin mediates skin fibroblast migration by promoting the formation of filopodia.

The deterministic lateral displacement (DLD) method has gradually moved beyond the separation of spherical rigid particles and has been successfully applied to the field of biological cells, especially to the separation of disc-shaped flexible erythrocytes. Different shapes of micropillars in the DLD device significantly affect the sorting performance of erythrocytes. We investigated the effect of the flow field in the gap of micropillars of different shapes (circular, triangular, I-shaped) on the behavior of erythrocyte tumbling motion. The simulation results showed that in the I-shaped pillar DLD device, compared with the circular and triangular pillars, the erythrocytes exhibited a stable and continuous flipping motion during the movement along the array offset direction, which enabled the effective separation size to be maintained at a high level. In addition, the formula for calculating the critical diameter of the I-shaped pillar DLD array for separating erythrocytes was obtained by fitting, which provides strong support for a more efficient design of the I-shaped pillar DLD device dedicated to erythrocyte sorting. [ABSTRACT FROM AUTHOR]

The development of flexible materials with higher piezoelectric properties and electrostrictive response is of great significance in many applications such as wearable functional devices, flexible sensors, and actuators. In this study, we report an efficient fabrication strategy to construct a highly sensitive (0.72 kPa -1 ), red light-emitting flexible pressure sensor using electrospun Eu 3+ -doped polyvinylidene fluoride-hexafluoropropylene/graphene oxide composite nanofibers using a layer-by-layer technology. The high β-phase concentration (96.3%) was achieved from the Eu 3+ -doped P(VDF-HFP)/GO nanofibers, leading to a high piezoelectricity of the composite nanofibers. We observed that a pressure sensor is enabled to generate an output voltage of 4.5 V. Furthermore, Eu 3+ -doped P(VDF-HFP)/GO composite nanofiber-based pressure sensors can also be used as an actuator as it has a good electrostrictive effect. At the same time, the nanofiber membrane has excellent ferroelectric properties and good fluorescence properties. These results indicate that this material has great application potential in the fields of photoluminescent fabrics, flexible sensors, soft actuators, and energy storage devices., Competing Interests: The authors declare no competing financial interest., (© 2022 The Authors. Published by American Chemical Society.)