123 results on '"Hoogland, G."'
Search Results
2. Visual field deficits after epilepsy surgery: a new quantitative scoring method
- Author
-
van Lanen, Rick H. G. J., Hoeberigs, M. C., Bauer, N. J. C., Haeren, R. H. L., Hoogland, G., Colon, A., Piersma, C., Dings, J. T. A., and Schijns, O. E. M. G.
- Published
- 2018
- Full Text
- View/download PDF
3. Value of ultra-high field MRI in patients with suspected focal epilepsy and negative 3 T MRI (EpiUltraStudy): protocol for a prospective, longitudinal therapeutic study
- Author
-
van Lanen, R. H. G. J., Wiggins, C. J., Colon, A. J., Backes, W. H., Jansen, J. F. A., Uher, D., Drenthen, G. S., Roebroeck, A., Ivanov, D., Poser, B. A., Hoeberigs, M. C., van Kuijk, S. M. J., Hoogland, G., Rijkers, K., Wagner, G. L., Beckervordersandforth, J., Delev, D., Clusmann, H., Wolking, S., Klinkenberg, S., Rouhl, R. P. W., Hofman, P. A. M., Schijns, O. E. M. G., RS: MHeNs - R3 - Neuroscience, Psychiatrie & Neuropsychologie, MUMC+: MA AIOS Neurochirurgie (9), Scannexus, RS: MHeNs - R1 - Cognitive Neuropsychiatry and Clinical Neuroscience, Beeldvorming, MUMC+: DA BV Klinisch Fysicus (9), RS: FPN CN 11, Multiscale Imaging of Brain Connectivity, MRI, RS: FPN CN 5, MUMC+: DA BV Medisch Specialisten Radiologie (9), RS: CAPHRI - R2 - Creating Value-Based Health Care, MUMC+: KIO Kemta (9), Epidemiologie, MUMC+: MA Niet Med Staf Neurochirurgie (9), MUMC+: MA Med Staf Spec Neurochirurgie (9), RS: GROW - R2 - Basic and Translational Cancer Biology, MUMC+: DA Pat Pathologie (9), Klinische Neurowetenschappen, and MUMC+: MA Med Staf Spec Neurologie (9)
- Subjects
SELECTION ,Drug Resistant Epilepsy ,SURGERY ,UHF MRI ,ILAE COMMISSION ,CONSENSUS CLASSIFICATION ,PROPOSAL ,Epilepsy surgery ,Humans ,Radiology, Nuclear Medicine and imaging ,Longitudinal Studies ,Prospective Studies ,INTRACTABLE EPILEPSY ,Child ,Epilepsy ,MEG ,SURGICAL OUTCOMES ,7 T ,Magnetic Resonance Imaging ,9.4 T ,CORTICAL DYSPLASIA ,Treatment Outcome ,Epilepsies, Partial ,Neurology (clinical) ,Cardiology and Cardiovascular Medicine ,HOC TASK-FORCE - Abstract
Purpose Resective epilepsy surgery is a well-established, evidence-based treatment option in patients with drug-resistant focal epilepsy. A major predictive factor of good surgical outcome is visualization and delineation of a potential epileptogenic lesion by MRI. However, frequently, these lesions are subtle and may escape detection by conventional MRI (≤ 3 T). Methods We present the EpiUltraStudy protocol to address the hypothesis that application of ultra-high field (UHF) MRI increases the rate of detection of structural lesions and functional brain aberrances in patients with drug-resistant focal epilepsy who are candidates for resective epilepsy surgery. Additionally, therapeutic gain will be addressed, testing whether increased lesion detection and tailored resections result in higher rates of seizure freedom 1 year after epilepsy surgery. Sixty patients enroll the study according to the following inclusion criteria: aged ≥ 12 years, diagnosed with drug-resistant focal epilepsy with a suspected epileptogenic focus, negative conventional 3 T MRI during pre-surgical work-up. Results All patients will be evaluated by 7 T MRI; ten patients will undergo an additional 9.4 T MRI exam. Images will be evaluated independently by two neuroradiologists and a neurologist or neurosurgeon. Clinical and UHF MRI will be discussed in the multidisciplinary epilepsy surgery conference. Demographic and epilepsy characteristics, along with postoperative seizure outcome and histopathological evaluation, will be recorded. Conclusion This protocol was reviewed and approved by the local Institutional Review Board and complies with the Declaration of Helsinki and principles of Good Clinical Practice. Results will be submitted to international peer-reviewed journals and presented at international conferences. Trial registration number www.trialregister.nl: NTR7536.
- Published
- 2022
- Full Text
- View/download PDF
4. Multimodal spatial omics in human focal epilepsy
- Author
-
Vermeulen, I., Dankcer, T., Hoogland, G., Rijkers, K., Schijns, O., Balluff, B., Cuypers, E., and Cillero-Pastor, B.
- Published
- 2022
- Full Text
- View/download PDF
5. Intraoperative assessment of the cerebrovascular glycocalyx and microcirculation in temporal lobe epilepsy
- Author
-
van Lanen, R.H.G.J., Haeren, R.H.L., Rijkers, K., Staals, J., van Zandvoort, M.A.M.J., Dings, J.T.A., Schijns, O.E.M.G., Hoogland, G., and Vink, H.
- Published
- 2021
- Full Text
- View/download PDF
6. Compleet en degelijk : Siloking TrailedLine 4.0 Premium
- Author
-
Hoogland, G., Rijnsburger, F., Hoogland, G., and Rijnsburger, F.
- Abstract
De TrailedLine 4.0 Premium-voermengwagen van Siloking is in vele varianten leverbaar. Maar ze zijn allemaal eenvoudig in gebruik, compleet uitgerust en degelijk gebouwd.
- Published
- 2020
7. Wakkere hersenchirurgie voor epilepsie: Preventie van taalstoornissen?
- Author
-
Joode, L.E.G.H. de, Haeren, R., Hendriks, M.P.H., Dings, J.T.A., Hoogland, G., Kranen-Mastenbroek, V.H.J.M. van, Hilkman, D.M.W., Schijns, O.E.M.G., Joode, L.E.G.H. de, Haeren, R., Hendriks, M.P.H., Dings, J.T.A., Hoogland, G., Kranen-Mastenbroek, V.H.J.M. van, Hilkman, D.M.W., and Schijns, O.E.M.G.
- Abstract
Item does not contain fulltext, Tijdens een wakkere hersenoperatie worden hersengebieden in kaart gebracht die betrokken zijn bij taalfuncties. In een onderzoek waarvan hier verslag wordt gedaan, werden van patiënten die in aanmerking kwamen voor epilepsiechirurgie de expressieve en receptieve taalfunctie en de aanvalsfrequentie pre- en postoperatief onderzocht. De resultaten tonen dat deze operatietechniek leidt tot een hoge kans op complete aanvalsvrijheid zonder verslechtering van taalfunctie.
- Published
- 2019
8. The expression of the distal dystrophin isoforms DP140 and DP71 in the human epileptic hippocampus in relation tot cognitive functioning
- Author
-
Hoogland, G., Hendriksen, R.G.F., Slegers, R.J., Hendriks, M.P.H., Schijns, O.E.M.G., Aalbers, M.W., Vles, J.S.H., Hoogland, G., Hendriksen, R.G.F., Slegers, R.J., Hendriks, M.P.H., Schijns, O.E.M.G., Aalbers, M.W., and Vles, J.S.H.
- Abstract
Item does not contain fulltext, Dystrophin is an important protein within the central nervous system. The absence of dystrophin, characterizing Duchenne Muscular Dystrophy (DMD), is associated with brain related comorbidities such as neurodevelopmental (e.g. cognitive and behavioural) deficits and epilepsy. Especially mutations in the downstream part of the DMD gene affecting the dystrophin isoforms Dp140 and Dp71 are found to be associated with cognitive deficits. However, the function of Dp140 is currently not well understood and its expression pattern has previously been implicated to be developmentally regulated. Therefore, we evaluated Dp140 and Dp71 expression in human hippocampi in relation to cognitive functioning in patients with drug-resistant temporal lobe epilepsy (TLE) and post-mortem controls. Hippocampal samples obtained as part of epilepsy surgery were quantitatively analyzed by Western blot and correlations with neuropsychological test results (i.e. memory and intelligence) were examined. First, we demonstrated that the expression of Dp140 does not appear to differ across different ages throughout adulthood. Second, we identified an inverse correlation between memory loss (i.e. verbal and visual memory), but not intelligence (i.e. neither verbal nor performance), and hippocampal Dp140 expression. Finally, patients with TLE appeared to have similar Dp140 expression levels compared to post-mortem controls without neurological disease. Dp140 may thus have a function in normal cognitive (i.e. episodic memory) processes.
- Published
- 2019
9. Distribution of glutamate transporters in the hippocampus of patients with pharmaco-resistant temporal lobe epilepsy
- Author
-
Proper, E. A., Hoogland, G., Kappen, S. M., Jansen, G. H., Rensen, M. G. A., Schrama, L. H., van Veelen, C. W. M., van Rijen, P. C., van Nieuwenhuizen, O., Gispen, W. H., and de Graan, P. N. E.
- Published
- 2002
10. Single-Cell Recordings to Target the Anterior Nucleus of the Thalamus in Deep Brain Stimulation for Patients with Refractory Epilepsy
- Author
-
Schaper, Frédéric L.W.V.J., Zhao, Yan, Janssen, Marcus L.F., Wagner, G. Louis, Colon, A.J., Hilkman, Danny M.W., Gommer, Erik, Vlooswijk, Mariëlle C.G., Hoogland, G., Ackermans, Linda, Bour, Lo J., Wezel, R.J.A. van, Boon, P.A.J.M., Temel, Yasin, Heida, Tjitske, Van Kranen-Mastenbroek, Vivianne H.J.M., Rouhl, Rob P.W., Schaper, Frédéric L.W.V.J., Zhao, Yan, Janssen, Marcus L.F., Wagner, G. Louis, Colon, A.J., Hilkman, Danny M.W., Gommer, Erik, Vlooswijk, Mariëlle C.G., Hoogland, G., Ackermans, Linda, Bour, Lo J., Wezel, R.J.A. van, Boon, P.A.J.M., Temel, Yasin, Heida, Tjitske, Van Kranen-Mastenbroek, Vivianne H.J.M., and Rouhl, Rob P.W.
- Abstract
Item does not contain fulltext
- Published
- 2018
11. In vivo assessment of the human cerebral microcirculation and its glycocalyx: A technical report
- Author
-
Haeren, R. H. L., Haeren, R. H. L., Rijkers, K., Schijns, O. E. M. G., Dings, J., Hoogland, G., van Zandvoort, M. A. M. J., Vink, H., van Overbeeke, J. J., Haeren, R. H. L., Haeren, R. H. L., Rijkers, K., Schijns, O. E. M. G., Dings, J., Hoogland, G., van Zandvoort, M. A. M. J., Vink, H., and van Overbeeke, J. J.
- Abstract
Introduction: The cerebral microcirculation and its glycocalyx, a matrix coating the luminal endothelium, are key regulators of capillary permeability and cerebral blood flow. Microvascular abnormalities are described in several neurological disorders. However, assessment of the cerebral microcirculation and glycocalyx has mainly been performed ex vivo. New method: Here, the technical feasibility of in vivo assessment of the human cerebral microcirculation and its glycocalyx using sidestream dark field (SDF) imaging is discussed. Intraoperative assessment requires the application of a sterile drape covering the camera (slipcover). First, sublingual measurements with and without slipcover were performed in a healthy control to assess the impact of this slipcover. Subsequently, using SDF imaging, the sublingual (reference), cortical, and hippocampal microcirculation and glycocalyx were evaluated in patients who underwent resective brain surgery as treatment for drug-resistant temporal lobe epilepsy. Finally, vessel density, and the perfused boundary region (PBR), a validated gauge of glycocalyx health, were calculated using GlycoCheck (c) software. Results: The addition of a slipcover affects vessel density and PBR values in a control subject. The cerebral measurements in five patients were more difficult to obtain than the sublingual ones. This was probably at least partly due to the introduction of a sterile slipcover. Results on vessel density and PBR showed similar patterns at all three measurement sites. Comparison with existing methods: This is the first report on in vivo assessment of the human cerebrovascular glycocalyx. Assessment of the glycocalyx is an additional application of in vivo imaging of the cerebral microcirculation using SDF technique. This method enables functional analysis of the microcirculation and glycocalyx, however the addition of a sterile slipcover affects the measurements. Conclusions: SDF imaging is a safe, quick, and straightforward t
- Published
- 2018
12. Histopathological findings in brain tissue obtained during epilepsy surgery
- Author
-
Blümcke, I., Spreafico, R., Haaker, G., Coras, R., Kobow, K., Bien, C.G., Pfäfflin, M., Elger, C., Widman, G., Schramm, J., Becker, A., Braun, K.P.J., Leijten, F.S.S., Baayen, J.C., Aronica, E., Chassoux, F., Hamer, H., Stefan, H., Rössler, K., Thom, M., Walker, M.C., Sisodiya, S.M., Duncan, J.S., McEvoy, A.W., Pieper, T., Holthausen, H., Kudernatsch, M., Meencke, H.J., Kahane, P., Schulze-Bonhage, A., Zentner, J., Heiland, D., Urbach, H., Steinhoff, B.J., Bast, T., Tassi, L., Lo Russo, G., Ozkara, C., Oz, B., Krsek, P., Vogelgesang, S., Runge, U., Lerche, H., Weber, Y., Honavar, M., Pimentel, J., Arzimanoglou, A., Ulate-Campos, A., Noachtar, S., Hartl, E., Schijns, O.E.M.G., Guerrini, R., Barba, C., Jacques, T.S., Cross, J.H., Feucht, M., Mühlebner, A., Grunwald, T., Trinka, E., Winkler, P.A., Gil-Nagel, A., Toledano Delgado, R., Mayer, T., Lutz, M., Zountsas, B., Garganis, K., Rosenow, F., Hermsen, A., Örtzen, T.J. von, Diepgen, T.L., Avanzini, G., Aparicio, J., Bento, C., Beckervordersandforth, J., Buccoliero, A.M., Cabral, P., Chamadoira, C., Colon, A.J., Chabardès, S., Carpenter, S., Czech, T., Dressler, A., Deleo, F., Dílio, A., Dings, J., Devaux, B., De Tisi, J., De Bellescize, J., Ebner, A., Franke, K., Groeppel, G., Giordano, F., Gozzo, F., Garbelli, R., Guenot, M., García‐Morales, I., Gómez‐Angulo, J.C., Garcia, G., Hainfellner, J.A., Höfler, J., Hoogland, G., Hendriks, M.P.H., Hofman, P., Harding, B., Huppertz, H.J., Herms, J., Hilkman, D.M.W., Hamelin, S., Idema, S., Jansen, F.E., Jahodova, A., Keeley, A., Kalss, G., Kudr, M., Kroell, J., Kokkinos, V., Keo Kosal, P., Kalbhenn, T., Leitinger, M., Landré, E., Melo Pires, M., Matas, A., Mann, M.W., Ostrowsky‐Coste, K., Prinz, M., Puttinger, G., Peraud, A., Rangel Pinho, R., Romero, C., Rego, R., Rouhl, R.P.W., Ryvlin, P., Rumia, J., Rampp, S., Scholl, T., Schulz, R., Stone, T.J., Streichenberger, N., Tisdall, M., Turak, B., Taipa, R., Uzan, M., Kranen‐Mastenbroek, V. van, Varlet, P., Vlooswijk, M.C.G., Wagner, L., Weis, S., Blümcke, I., Spreafico, R., Haaker, G., Coras, R., Kobow, K., Bien, C.G., Pfäfflin, M., Elger, C., Widman, G., Schramm, J., Becker, A., Braun, K.P.J., Leijten, F.S.S., Baayen, J.C., Aronica, E., Chassoux, F., Hamer, H., Stefan, H., Rössler, K., Thom, M., Walker, M.C., Sisodiya, S.M., Duncan, J.S., McEvoy, A.W., Pieper, T., Holthausen, H., Kudernatsch, M., Meencke, H.J., Kahane, P., Schulze-Bonhage, A., Zentner, J., Heiland, D., Urbach, H., Steinhoff, B.J., Bast, T., Tassi, L., Lo Russo, G., Ozkara, C., Oz, B., Krsek, P., Vogelgesang, S., Runge, U., Lerche, H., Weber, Y., Honavar, M., Pimentel, J., Arzimanoglou, A., Ulate-Campos, A., Noachtar, S., Hartl, E., Schijns, O.E.M.G., Guerrini, R., Barba, C., Jacques, T.S., Cross, J.H., Feucht, M., Mühlebner, A., Grunwald, T., Trinka, E., Winkler, P.A., Gil-Nagel, A., Toledano Delgado, R., Mayer, T., Lutz, M., Zountsas, B., Garganis, K., Rosenow, F., Hermsen, A., Örtzen, T.J. von, Diepgen, T.L., Avanzini, G., Aparicio, J., Bento, C., Beckervordersandforth, J., Buccoliero, A.M., Cabral, P., Chamadoira, C., Colon, A.J., Chabardès, S., Carpenter, S., Czech, T., Dressler, A., Deleo, F., Dílio, A., Dings, J., Devaux, B., De Tisi, J., De Bellescize, J., Ebner, A., Franke, K., Groeppel, G., Giordano, F., Gozzo, F., Garbelli, R., Guenot, M., García‐Morales, I., Gómez‐Angulo, J.C., Garcia, G., Hainfellner, J.A., Höfler, J., Hoogland, G., Hendriks, M.P.H., Hofman, P., Harding, B., Huppertz, H.J., Herms, J., Hilkman, D.M.W., Hamelin, S., Idema, S., Jansen, F.E., Jahodova, A., Keeley, A., Kalss, G., Kudr, M., Kroell, J., Kokkinos, V., Keo Kosal, P., Kalbhenn, T., Leitinger, M., Landré, E., Melo Pires, M., Matas, A., Mann, M.W., Ostrowsky‐Coste, K., Prinz, M., Puttinger, G., Peraud, A., Rangel Pinho, R., Romero, C., Rego, R., Rouhl, R.P.W., Ryvlin, P., Rumia, J., Rampp, S., Scholl, T., Schulz, R., Stone, T.J., Streichenberger, N., Tisdall, M., Turak, B., Taipa, R., Uzan, M., Kranen‐Mastenbroek, V. van, Varlet, P., Vlooswijk, M.C.G., Wagner, L., and Weis, S.
- Abstract
Item does not contain fulltext, Background: Detailed neuropathological information on the structural brain lesions underlying seizures is valuable for understanding drug-resistant focal epilepsy. Methods: We report the diagnoses made on the basis of resected brain specimens from 9523 patients who underwent epilepsy surgery for drug-resistant seizures in 36 centers from 12 European countries over 25 years. Histopathological diagnoses were determined through examination of the specimens in local hospitals (41%) or at the German Neuropathology Reference Center for Epilepsy Surgery (59%). Results: The onset of seizures occurred before 18 years of age in 75.9% of patients overall, and 72.5% of the patients underwent surgery as adults. The mean duration of epilepsy before surgical resection was 20.1 years among adults and 5.3 years among children. The temporal lobe was involved in 71.9% of operations. There were 36 histopathological diagnoses in seven major disease categories. The most common categories were hippocampal sclerosis, found in 36.4% of the patients (88.7% of cases were in adults), tumors (mainly ganglioglioma) in 23.6%, and malformations of cortical development in 19.8% (focal cortical dysplasia was the most common type, 52.7% of cases of which were in children). No histopathological diagnosis could be established for 7.7% of the patients. Conclusions: In patients with drug-resistant focal epilepsy requiring surgery, hippocampal sclerosis was the most common histopathological diagnosis among adults, and focal cortical dysplasia was the most common diagnosis among children. Tumors were the second most common lesion in both groups. (Funded by the European Union and others.)
- Published
- 2017
13. Protocol for intraoperative assessment of the human cerebrovascular glycocalyx
- Author
-
Haeren, R. H. L., Haeren, R. H. L., Vink, H., Staals, J., van Zandvoort, M. A. M. J., Dings, J., van Overbeeke, J. J., Hoogland, G., Rijkers, K., Schijns, O. E. M. G., Haeren, R. H. L., Haeren, R. H. L., Vink, H., Staals, J., van Zandvoort, M. A. M. J., Dings, J., van Overbeeke, J. J., Hoogland, G., Rijkers, K., and Schijns, O. E. M. G.
- Abstract
Introduction: Adequate functioning of the blood-brain barrier (BBB) is important for brain homoeostasis and normal neuronal function. Disruption of the BBB has been described in several neurological diseases. Recent reports suggest that an increased permeability of the BBB also contributes to increased seizure susceptibility in patients with epilepsy. The endothelial glycocalyx is coating the luminal side of the endothelium and can be considered as the first barrier of the BBB. We hypothesise that an altered glycocalyx thickness plays a role in the aetiology of temporal lobe epilepsy (TLE), the most common type of epilepsy. Here, we propose a protocol that allows intraoperative assessment of the cerebrovascular glycocalyx thickness in patients with TLE and assess whether its thickness is decreased in patients with TLE when compared with controls.Methods and analysis: This protocol is designed as a prospective observational case-control study in patients who undergo resective brain surgery as treatment for TLE. Control subjects are patients without a history of epileptic seizures, who undergo a craniotomy or burr hole surgery for other indications. Intraoperative glycocalyx thickness measurements of sublingual, cortical and hippocampal microcirculation are performed by video microscopy using sidestream dark-field imaging. Demographic details, seizure characteristics, epilepsy risk factors, intraoperative haemodynamic parameters and histopathological evaluation are additionally recorded.Ethics and dissemination: This protocol has been ethically approved by the local medical ethical committee (ID: NL51594.068.14) and complies with the Declaration of Helsinki and principles of Good Clinical Practice. Informed consent is obtained before study enrolment and only coded data will be stored in a secured database, enabling an audit trail. Results will be submitted to international peer-reviewed journals and presented at international conferences.
- Published
- 2017
14. Validation of reference genes in human chordoma
- Author
-
Temel, Y, primary, Santegoeds, R. G. C., additional, Yakkioui, Y, additional, Jahanshahi, A, additional, Hoogland, G, additional, and van Overbeeke, JJ, additional
- Published
- 2017
- Full Text
- View/download PDF
15. Protocol for intraoperative assessment of the human cerebrovascular glycocalyx
- Author
-
Haeren, R H L, primary, Vink, H, additional, Staals, J, additional, van Zandvoort, M A M J, additional, Dings, J, additional, van Overbeeke, J J, additional, Hoogland, G, additional, Rijkers, K, additional, and Schijns, O E M G, additional
- Published
- 2017
- Full Text
- View/download PDF
16. Hippocampal GABA transporter distribution in patients with temporal lobe epilepsy and hippocampal sclerosis
- Author
-
Schijns, O., Karaca, U., Andrade, P., Nijs, L. de, Kusters, B., Peeters, A., Dings, J., Pannek, H., Ebner, A., Rijkers, K., Hoogland, G., Schijns, O., Karaca, U., Andrade, P., Nijs, L. de, Kusters, B., Peeters, A., Dings, J., Pannek, H., Ebner, A., Rijkers, K., and Hoogland, G.
- Abstract
Item does not contain fulltext, PURPOSE: To determine hippocampal expression of neuronal GABA-transporter (GAT-1) and glial GABA-transporter (GAT-3) in patients with temporal lobe epilepsy (TLE) and hippocampal sclerosis (HS). METHODS: Hippocampal sections were immunohistochemically stained for GABA-transporter 1 and GABA-transporter-3, followed by quantification of the immunoreactivity in the hilus by optical density measurements. GABA-transporter 3 positive hilar cells were counted and GABA-transporter protein expression in sections that included all hippocampal subfields was quantified by Western blot. RESULTS: The hilar GABA-transporter 1 expression of patients with severe hippocampal sclerosis was about 7% lower compared to that in the mild hippocampal sclerosis/control group (p<0.001). The hilar GABA-transporter 3 expression was about 5% lower in the severe hippocampal sclerosis group than in the mild hippocampal sclerosis/control group (non-significant). Also, severe hippocampal sclerosis samples contained 34% less (non-significant) GABA-transporter 3 positive cells compared to that of controls. Protein expression as assessed by Western blot showed that GABA-transporter 1 was equally expressed in mild and severe hippocampal sclerosis samples, whereas GABA-transporter 3 was reduced by about 62% in severe hippocampal sclerosis samples (p<0.0001). CONCLUSION: These data confirm that GABA-transporter expression is spatially and isoform-specific reduced and GABA-transporter 3 positive cell numbers are unchanged in hippocampal sclerosis. Implications for the use of GABAergic antiepileptic therapies in hippocampal sclerosis vs non-hippocampal sclerosis patients remain to be studied.
- Published
- 2015
17. Glutamaat- en GABA-afgifte uit zenuwuiteinden van patiënten met medicamenteus onbehandelbare temporaalkwab-epilepsie
- Author
-
Gispen, W.H., Hoogland, G., Veelen, C.W. van, Rijen, P.C. van, Huffelen, A.C. van, and Graan, P.N.E. de
- Subjects
Geneeskunde - Published
- 1999
18. Polymorphisms in CACNA1E and Camk2d are associated with seizure susceptibility of Sprague-Dawley rats.
- Author
-
Rijkers, K., Mescheriakova, J., Majoie, M., Lemmens, E., Wijk, X.M.R. van, Philippens, M., Kranen-Mastenbroek, V.H. van, Schijns, O., Vles, J., Hoogland, G., Rijkers, K., Mescheriakova, J., Majoie, M., Lemmens, E., Wijk, X.M.R. van, Philippens, M., Kranen-Mastenbroek, V.H. van, Schijns, O., Vles, J., and Hoogland, G.
- Abstract
1 september 2010, Item does not contain fulltext, Seizures are associated with high intracellular calcium levels. However, conditions characterized by high intracellular calcium levels, such as stroke or traumatic brain injury, do not always evoke epilepsy. We hypothesized that polymorphisms in calcium-related genes CACNA1E and Camk2d contribute to the individual variability in seizure susceptibility. The distribution of one single nucleotide polymorphism (SNP) in the CACNA1E and one in the Camk2d gene was determined in Sprague-Dawley rats that were subjected to amygdala kindling or hyperthermia-induced seizures. The pre-kindling afterdischarge threshold was significantly lower in rats with the CACNA1E GG genotype (45.2+/-6.7microA) than in the GT genotyped animals (79.3+/-53.7microA). Among hyperthermia treated rats, the Camk2d G allele was more frequent among rats that did not display behavioral seizures during hyperthermia (67%) than in animals that did show behavioral seizures during hyperthermia (52%, chi(2)(1)=3.847, p=0.05). SNPs in CACNA1E and Camk2d genes are associated with the individual variability in seizure susceptibility in two experimental seizure models.
- Published
- 2010
19. Principe ontwerpen natuurvriendelijke oevers
- Author
-
Pelsma, T.A.H.M. (author), Weenink, E. (author), Hoogland, G. (author), Pelsma, T.A.H.M. (author), Weenink, E. (author), and Hoogland, G. (author)
- Abstract
In opdracht van de afdeling Planvorming van de Sector Watersystemen, is een studie opgezet om te komen tot standaard ontwerpen voor natuurvriendelijke oevers. In het beheersgebied van hoogheemraadschap Amstel, Gooi en Vecht (AGV) zullen de komende tijd flinke stukken waterkering of kades worden verstevigd. Zowel binnen- als buiten de ecologische hoofdstructuur (EHS) doen zich hierbij kansen voor om de oevers meer natuurvriendelijk in te richten. Daarnaast kan het aanleggen van een natuurvriendelijke oever ook een KRW maatregel zijn, die vanaf 2010 in uitvoering komen. Dit kan eveneens langs boezemwateren zijn, maar ook langs andere wateren van AGV, zoals meren en sloten. Om een ontwerp te kunnen laten slagen, zijn er vanuit de ecologie randvoorwaarden te verbinden aan het ontwerp, veelal betreft dit de onderwaterhelling, de bodemsoort en een effectieve bescherming tegen golven. De ecologische functie is niet de enige functie van een oever, maar is bij EHS verbindingen meer prioritair dan elders. In de hier gepresenteerde ontwerpen is zeker rekening gehouden met andere functies zoals waterberging, doorstroming en recreatie. Voordat tot aanleg van een natuurvriendelijke oever wordt overgegaan, dient eerst te worden bepaald of zon oever op de beoogde locaties wel op zijn plaats is. En zo ja welke van de ontwerpen dan het meest passend is. Om dit te faciliteren zijn er beslisbomen in het rapport opgenomen. Daarbij wordt onderscheid gemaakt tussen wateren in de stad en daarbuiten en tussen wel en geen EHS oevers. De aanleg van een natuurvriendelijke oever is een cruciaal moment. De te gebruiken bodemsoorten en de wijze van vergraven maken veel uit. Niet altijd kan ter plaatse aanwezige grond goed worden gebruikt en soms dient grond van elders te worden gehaald. Aan deze grond zijn natuurtechnische eisen te verbinden. O.a aan lutum gehalte en organisch stof gehalte. Het op depot zetten van aanwezige vegetatie, in het bijzonder riet, en deze weer terug zetten bij opleve
- Published
- 2009
20. Surgery for temporal lobe epilepsy after cerebral malaria
- Author
-
Schijns, O.E.M.G., Visser-Vandewalle, V., Lemmens, E.M.P., Janssen, A., and Hoogland, G.
- Published
- 2008
- Full Text
- View/download PDF
21. Distribution of glutamate transporters in the hippocampus of patients with pharmaco-resistant temporal lobe epilepsy
- Author
-
Gispen, W.H., Proper, E.A., Hoogland, G., Kappen, S.M., Jansen, G.H., Rensen, M.G., Schrama, L.H., Veelen, C.W. van, Rijen, P.C. van, Nieuwenhuizen, O. van, Graan, P.N. de, Gispen, W.H., Proper, E.A., Hoogland, G., Kappen, S.M., Jansen, G.H., Rensen, M.G., Schrama, L.H., Veelen, C.W. van, Rijen, P.C. van, Nieuwenhuizen, O. van, and Graan, P.N. de
- Published
- 2002
22. Characterization of neocortical and hippocampal synaptosomes from temporal lobe epilepsy patients
- Author
-
Gispen, W.H., Hoogland, G., Blomenrohr, M., Dijstelbloem, H.M., Wit, de M., Spierenburg, H.A., Veelen, C.W. van, Rijen, P.C. van, Huffelen, A.C. van, Graan, P.N. de, Gispen, W.H., Hoogland, G., Blomenrohr, M., Dijstelbloem, H.M., Wit, de M., Spierenburg, H.A., Veelen, C.W. van, Rijen, P.C. van, Huffelen, A.C. van, and Graan, P.N. de
- Published
- 1999
23. Phosphoproteins and the regulation of vesicular neurotransmitter release
- Author
-
Gispen, W.H., Hens, J.J.H., Hoogland, G., Graan, P.N.E. de, Gispen, W.H., Hens, J.J.H., Hoogland, G., and Graan, P.N.E. de
- Published
- 1997
24. Diurnal effects of motor activity and fatigue in Parkinson's disease.
- Author
-
van Hilten, J J, primary, Hoogland, G, additional, van der Velde, E A, additional, Middelkoop, H A, additional, Kerkhof, G A, additional, and Roos, R A, additional
- Published
- 1993
- Full Text
- View/download PDF
25. Cerebrovascular glycocalyx damage and microcirculation impairment in patients with temporal lobe epilepsy.
- Author
-
van Lanen RH, Haeren RH, Staals J, Dings JT, Schijns OE, Hoogland G, van Kuijk SM, Kapsokalyvas D, van Zandvoort MA, Vink H, and Rijkers K
- Subjects
- Humans, Glycocalyx, Microcirculation physiology, Blood-Brain Barrier, Capillaries, Epilepsy, Temporal Lobe surgery
- Abstract
Temporal lobe epilepsy (TLE) is increasingly associated with blood-brain barrier dysfunction and microvascular alterations, yet the pathophysiological link is missing. An important barrier function is exerted by the glycocalyx, a gel-like layer coating the endothelium. To explore such associations, we used intraoperative videomicroscopy to quantify glycocalyx and microcirculation properties of the neocortex and hippocampus of 15 patients undergoing resective brain surgery as treatment for drug-resistant TLE, and 15 non-epileptic controls. Fluorescent lectin staining of neocortex and hippocampal tissue was used for blood vessel surface area quantification. Neocortical perfused boundary region, the thickness of the glycocalyx' impaired layer, was higher in patients (2.64 ± 0.52 µm) compared to controls (1.31 ± 0.29 µm), P < 0.01, indicative of reduced glycocalyx integrity in patients. Moreover, erythrocyte flow velocity analysis revealed an impaired ability of TLE patients to (de-)recruit capillaries in response to changing metabolic demands ( R
2 = 0.75, P < 0.01), indicating failure of neurovascular coupling mechanisms. Blood vessel quantification comparison between intraoperative measurements and resected tissue showed strong correlation ( R2 = 0.94, P < 0.01). This is the first report on in vivo assessment of glycocalyx and microcirculation properties in TLE patients, confirming the pivotal role of cerebrovascular changes. Further assessment of the cerebral microcirculation in relation to epileptogenesis might open avenues for new therapeutic targets for drug-resistant epilepsy., Competing Interests: Declaration of conflicting interestsThe author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: HV is the Chief Science Officer at GlycoCheck & Microvascular Health Solutions. The authors declare that this manuscript has not been or currently is under review by any other journal.- Published
- 2023
- Full Text
- View/download PDF
26. Resective Epilepsy Surgery, QUality of life and Economic evaluation (RESQUE): the change in quality of life after resective epilepsy surgery-protocol for a multicentre, prospective cohort study.
- Author
-
Kellenaers JTF, Rijkers K, van Mastrigt GAPG, Schijns OEMG, Hoogland G, Dings J, van Kuijk S, Vlooswijk MCG, Wagner LGL, Idema S, van Straaten IECW, van der Salm SMA, and Majoie MHJM
- Subjects
- Humans, Cohort Studies, Cost-Benefit Analysis, Multicenter Studies as Topic, Prospective Studies, Quality of Life, Seizures, Treatment Outcome, Drug Resistant Epilepsy surgery, Epilepsy surgery, Epilepsy complications
- Abstract
Introduction: Resective epilepsy surgery is often seen as a last resort when treating drug-resistant epilepsy. Positive results on quality of life (QoL) and economic benefits after surgery argue for a less restrictive attitude towards epilepsy surgery for drug-resistant epilepsy. QoL and economic benefits are country-dependent. The objective of the Resective Epilepsy Surgery, QUality of life and Economic evaluation (RESQUE) trial is to evaluate the change in QoL before and after epilepsy surgery in Dutch people with drug-resistant epilepsy. The results will form part of an economic evaluation of epilepsy surgery in people with epilepsy (PWE) in The Netherlands., Methods and Analysis: A longitudinal prospective multicentre cohort study involving 100 PWE undergoing epilepsy surgery between 2019 and 2025 is being performed in three Dutch academic hospitals. Excluded are PWE who have a lower level of intelligence (TIQ<70) or who do not master the Dutch language. Before surgery and 3, 6, 12 and 24 months after surgery, PWE receive validated online questionnaires (QOLIE-31, EQ-5D, iMCQ and iPCQ) on QoL, cost of care, expectations and satisfaction. Primary outcome is the change in QoL. Secondary outcomes are change in generic QoL, seizure reduction (International League Against Epilepsy Outcome Classification), medical consumption, productivity, the correlation between QoL and seizure reduction and expectation of and satisfaction with the surgery., Ethics and Dissemination: The study design has been approved by the Medical Ethics Review Committee (METC) of Maastricht UMC+ (2019-1134) and the Amsterdam UMC (vu). At the time of writing, UMC Utrecht is in the process of considering approval. The study will be conducted according to the Dutch Medical Research Involving Human Subjects Act and the Declaration of Helsinki. The results will be publicly disclosed and submitted for publication in international peer-reviewed scientific journals. There is no veto on publication by the involved parties., Trial Registration: NL8278; Pre-results., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2023
- Full Text
- View/download PDF
27. Visualizing GABA transporters in vivo: an overview of reported radioligands and future directions.
- Author
-
Knippenberg N, Bauwens M, Schijns O, Hoogland G, Florea A, Rijkers K, Cleij TJ, Eersels K, van Grinsven B, and Diliën H
- Abstract
By clearing GABA from the synaptic cleft, GABA transporters (GATs) play an essential role in inhibitory neurotransmission. Consequently, in vivo visualization of GATs can be a valuable diagnostic tool and biomarker for various psychiatric and neurological disorders. Not surprisingly, in recent years several research attempts to develop a radioligand have been conducted, but so far none have led to suitable radioligands that allow imaging of GATs. Here, we provide an overview of the radioligands that were developed with a focus on GAT1, since this is the most abundant transporter and most of the research concerns this GAT subtype. Initially, we focus on the field of GAT1 inhibitors, after which we discuss the development of GAT1 radioligands based on these inhibitors. We hypothesize that the radioligands developed so far have been unsuccessful due to the zwitterionic nature of their nipecotic acid moiety. To overcome this problem, the use of non-classical GAT inhibitors as basis for GAT1 radioligands or the use of carboxylic acid bioisosteres may be considered. As the latter structural modification has already been used in the field of GAT1 inhibitors, this option seems particularly viable and could lead to the development of more successful GAT1 radioligands in the future., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
28. Experimental early-life febrile seizures cause a sustained increase in excitatory neurotransmission in newborn dentate granule cells.
- Author
-
Hoogland G, Raijmakers M, Clynen E, Brône B, Rigo JM, and Swijsen A
- Subjects
- Animals, Dentate Gyrus metabolism, Fever, Neurons metabolism, Rats, Rats, Sprague-Dawley, Synaptic Transmission, Seizures, Febrile, Status Epilepticus
- Abstract
Prolonged febrile seizures (FS) are a risk factor for the development of hippocampal-associated temporal lobe epilepsy. The dentate gyrus is the major gateway to the hippocampal network and one of the sites in the brain where neurogenesis continues postnatally. Previously, we found that experimental FS increase the survival rate and structural integration of newborn dentate granule cells (DGCs). In addition, mature post-FS born DGCs express an altered receptor panel. Here, we aimed to study if these molecular and structural changes are accompanied by an altered cellular functioning. Experimental FS were induced by hyperthermia in 10-days-old Sprague-Dawley rats. Proliferating progenitor cells were labeled the next day by injecting green fluorescent protein expressing retroviral particles bilaterally in the dentate gyri. Eight weeks later, spontaneous excitatory and inhibitory postsynaptic events (sEPSCs and sIPSCs, respectively) were recorded from labeled DGCs using the whole-cell patch-clamp technique. Experimental FS resulted in a robust decrease of the inter event interval (p < .0001) and a small decrease of the amplitude of sEPSCs (p < .001). Collectively the spontaneous excitatory charge transfer increased (p < .01). Experimental FS also slightly increased the frequency of sIPSCs (p < .05), while the amplitude of these events decreased strongly (p < .0001). The net inhibitory charge transfer remained unchanged. Experimental, early-life FS have a long-term effect on post-FS born DGCs, as they display an increased spontaneous excitatory input when matured. It remains to be established if this presents a mechanism for FS-induced epileptogenesis., (© 2022 The Authors. Brain and Behavior published by Wiley Periodicals LLC.)
- Published
- 2022
- Full Text
- View/download PDF
29. From a dysembryoplastic neuroepithelial tumor to a glioblastoma multiforme: Pitfalls of initial diagnosis on biopsy material, a case report.
- Author
-
Slegers RJ, Beckervordersandforth J, Hoeben A, Hoogland G, Broen MPG, Anten M, Dings JTA, van den Ende P, Henneman WJP, and Schijns OEMG
- Abstract
Background: Ganglioglioma (GG) and dysembryoplastic neuroepithelial tumor (DNET) belong to the group of low-grade epilepsy-associated tumors (LEAT) and are the most prevalent tumor types found in patients undergoing epilepsy surgery. Histopathological differentiation between GG and DNET can be difficult on biopsies due to limited tumor tissue., Case Description: Here, we present a rare case where a low-grade tumor was initially classified as DNET, based on biopsy findings and unfortunately dedifferentiated within 10 years into a glioblastoma multiforme. After gross total resection, the initial tumor was reclassified as GG., Conclusion: This case illustrates the diagnostic challenges of LEAT, especially on biopsy material. Therefore, we advocate to counsel for complete resection and histopathological diagnosis utilizing tumor markers to confirm the nature of the tumor and to advice type of follow-up and eventual concurrent treatment., Competing Interests: There are no conflicts of interest., (Copyright: © 2022 Surgical Neurology International.)
- Published
- 2022
- Full Text
- View/download PDF
30. Dystrophin in the Neonatal and Adult Rat Intestine.
- Author
-
Lionarons JM, Hoogland G, Slegers RJ, Steinbusch H, Claessen SMH, and Vles JSH
- Abstract
Background: Gastrointestinal (GI) complaints are frequently noted in aging dystrophinopathy patients, yet their underlying molecular mechanisms are largely unknown. As dystrophin protein isoform 71 (Dp71) is particularly implicated in the development of smooth muscle cells, we evaluated its distribution in the neonatal and adult rat intestine in this study., Methods: Dp71 expression levels were assessed in the proximal (duodenum, jejunum and ileum) and distal (caecum, colon and rectum) intestine by Western blotting and qPCR. In addition, the cellular distribution of total Dp was evaluated in the duodenum and colon by immunohistochemical colocalization studies with alpha-smooth muscle actin (aSMA), Hu RNA binding proteins C and D (HuC/HuD) for neurons and vimentin (VIM) for interstitial cells., Results: In neonatal and adult rats, the distal intestine expressed 2.5 times more Dp71 protein than the proximal part ( p < 0.01). This regional difference was not observed in Dp71 mRNA. During both stages, Dp-immunoreactivity was predominant in the muscularis propria, where it co-localized with aSMA and HuC/HuD., Conclusions: In neonatal and adult rats, Dp71 was expressed highest in the distal intestine. Together with the observation that Dp may be expressed by myenteric neurons, this warrants a paradigm shift in the treatment of GI comorbidities.
- Published
- 2021
- Full Text
- View/download PDF
31. Microvascular changes associated with epilepsy: A narrative review.
- Author
-
van Lanen RH, Melchers S, Hoogland G, Schijns OE, Zandvoort MAV, Haeren RH, and Rijkers K
- Subjects
- Female, Humans, Male, Blood-Brain Barrier physiopathology, Epilepsy blood, Microcirculation physiology
- Abstract
The blood-brain barrier (BBB) is dysfunctional in temporal lobe epilepsy (TLE). In this regard, microvascular changes are likely present. The aim of this review is to provide an overview of the current knowledge on microvascular changes in epilepsy, and includes clinical and preclinical evidence of seizure induced angiogenesis, barriergenesis and microcirculatory alterations. Anatomical studies show increased microvascular density in the hippocampus, amygdala, and neocortex accompanied by BBB leakage in various rodent epilepsy models. In human TLE, a decrease in afferent vessels, morphologically abnormal vessels, and an increase in endothelial basement membranes have been observed. Both clinical and experimental evidence suggests that basement membrane changes, such as string vessels and protrusions, indicate and visualize a misbalance between endothelial cell proliferation and barriergenesis. Vascular endothelial growth factor (VEGF) appears to play a crucial role. Following an altered vascular anatomy, its physiological functioning is affected as expressed by neurovascular decoupling that subsequently leads to hypoperfusion, disrupted parenchymal homeostasis and potentially to seizures". Thus, epilepsy might be a condition characterized by disturbed cerebral microvasculature in which VEGF plays a pivotal role. Additional physiological data from patients is however required to validate findings from models and histological studies on patient biopsies.
- Published
- 2021
- Full Text
- View/download PDF
32. The Role of the Glycocalyx in the Pathophysiology of Subarachnoid Hemorrhage-Induced Delayed Cerebral Ischemia.
- Author
-
Schenck H, Netti E, Teernstra O, De Ridder I, Dings J, Niemelä M, Temel Y, Hoogland G, and Haeren R
- Abstract
The glycocalyx is an important constituent of blood vessels located between the bloodstream and the endothelium. It plays a pivotal role in intercellular interactions in neuroinflammation, reduction of vascular oxidative stress, and provides a barrier regulating vascular permeability. In the brain, the glycocalyx is closely related to functions of the blood-brain barrier and neurovascular unit, both responsible for adequate neurovascular responses to potential threats to cerebral homeostasis. An aneurysmal subarachnoid hemorrhage (aSAH) occurs following rupture of an intracranial aneurysm and leads to immediate brain damage (early brain injury). In some cases, this can result in secondary brain damage, also known as delayed cerebral ischemia (DCI). DCI is a life-threatening condition that affects up to 30% of all aSAH patients. As such, it is associated with substantial societal and healthcare-related costs. Causes of DCI are multifactorial and thought to involve neuroinflammation, oxidative stress, neuroinflammation, thrombosis, and neurovascular uncoupling. To date, prediction of DCI is limited, and preventive and effective treatment strategies of DCI are scarce. There is increasing evidence that the glycocalyx is disrupted following an aSAH, and that glycocalyx disruption could precipitate or aggravate DCI. This review explores the potential role of the glycocalyx in the pathophysiological mechanisms contributing to DCI following aSAH. Understanding the role of the glycocalyx in DCI could advance the development of improved methods to predict DCI or identify patients at risk for DCI. This knowledge may also alter the methods and timing of preventive and treatment strategies of DCI. To this end, we review the potential and limitations of methods currently used to evaluate the glycocalyx, and strategies to restore or prevent glycocalyx shedding., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Schenck, Netti, Teernstra, De Ridder, Dings, Niemelä, Temel, Hoogland and Haeren.)
- Published
- 2021
- Full Text
- View/download PDF
33. Neuroimaging Detectable Differences between Parkinson's Disease Motor Subtypes: A Systematic Review.
- Author
-
Boonstra JT, Michielse S, Temel Y, Hoogland G, and Jahanshahi A
- Abstract
Background: The neuroanatomical substrates of Parkinson's disease (PD) with tremor-dominance (TD) and those with non-tremor dominance (nTD), postural instability and gait difficulty (PIGD), and akinetic-rigid (AR) are not fully differentiated. A better understanding of symptom specific pathoanatomical markers of PD subtypes may result in earlier diagnosis and more tailored treatment. Here, we aim to give an overview of the neuroimaging literature that compared PD motor subtypes., Methods: A systematic literature review on neuroimaging studies of PD subtypes was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Search terms submitted to the PubMed database included: "Parkinson's disease", "MRI" and "motor subtypes" (TD, nTD, PIGD, AR). The results are first discussed from macro to micro level of organization (i.e., (1) structural; (2) functional; and (3) molecular) and then by applied imaging methodology., Findings: Several neuroimaging methods including diffusion imaging and positron emission tomography (PET) distinguish specific PD motor subtypes well, although findings are mixed. Furthermore, our review demonstrates that nTD-PD patients have more severe neuroalterations compared to TD-PD patients. More specifically, nTD-PD patients have deficits within striato-thalamo-cortical (STC) circuitry and other thalamocortical projections related to cognitive and sensorimotor function, while TD-PD patients tend to have greater cerebello-thalamo-cortical (CTC) circuitry dysfunction., Conclusions: Based on the literature, STC and CTC circuitry deficits seem to be the key features of PD and the subtypes. Future research should make greater use of multimodal neuroimaging and techniques that have higher sensitivity in delineating subcortical structures involved in motor diseases., (© 2020 The Authors. Movement Disorders Clinical Practice published by Wiley Periodicals LLC. on behalf of International Parkinson and Movement Disorder Society.)
- Published
- 2020
- Full Text
- View/download PDF
34. Radionuclide tumor necrosis factor-alpha activity in herniated lumbar disc correlates with severe leg pain.
- Author
-
Andrade P, van Aalst J, Bauwens M, Vogg A, van Kroonenburgh MJ, Mottaghy FM, Teernstra OP, and Hoogland G
- Abstract
Background: Lumbar disc herniation is often associated with an inflammatory process. In this context, inflammation has been considered a key factor in the modulation of pain. Here, we present a case of inflammatory activity directly documented in a patient with a lumbar disc herniation., Case Description: A 49-year-old male presented with progressive low back pain and left-sided S1 radiculopathy, without a focal neurological deficit. The lumbar MR revealed a prominent herniated disc at the L5-S1 level, with compression of the left S1 root. The patient underwent a L5-S1 discectomy using a standard interlaminar approach. Although initially he was pain free, he required three additional operations to address recurrent pain complaints. As research indicates that local inflammation contributes to neuropathic pain, we had the patient undergoes single-photon emission computed tomography (SPECT) imaging using technetium-99m-labeled-infliximab (an anti-tumor necrosis factor [TNF]-alpha monoclonal antibody) before a proposed fourth operation. The SPECT study documented a strong signal at the site of the herniated disc, thus confirming the diagnosis of a pro-inflammatory process involving the S1 nerve root. Nine months after the fourth operation, the patient was pain free. Of interest, the second SPECT study in the now asymptomatic patient demonstrated no detectable/ residual signal at the operative/disc site., Conclusion: Absence of a SPECT TNF-alpha signal in a pain-free patient following a lumbar discectomy correlates with the reduction/resolution of the local preoperative inflammatory response., Competing Interests: There are no conflicts of interest., (Copyright: © 2020 Surgical Neurology International.)
- Published
- 2020
- Full Text
- View/download PDF
35. Assessing the effectiveness of perioperative s-ketamine on new-onset headache after resective epilepsy surgery (ESPAIN-trial): protocol for a randomised, double-blind, placebo-controlled trial.
- Author
-
Sloekers JCT, Bos M, Hoogland G, Bastiaenen C, van Kuijk S, Theunissen M, Rijkers K, Dings J, Colon A, Rouhl RPW, and Schijns OEMG
- Subjects
- Double-Blind Method, Drug Combinations, Headache etiology, Humans, Neurosurgical Procedures adverse effects, Pain, Postoperative etiology, Perioperative Care, Treatment Outcome, Acetaminophen therapeutic use, Analgesics, Non-Narcotic therapeutic use, Analgesics, Opioid therapeutic use, Epilepsy surgery, Headache drug therapy, Ketamine therapeutic use, Pain, Postoperative drug therapy, Randomized Controlled Trials as Topic methods
- Abstract
Introduction: Effective treatment of new-onset headache after craniotomy, especially anterior temporal lobectomy (ATL) and amygdalohippocampectomy for drug-resistant temporal lobe epilepsy, is a challenge. The current practice, acetaminophen combined with opioids is often reported by patients as insufficient and sometimes accompanied by opioid-related adverse effects. Based on expert opinion, anaesthesiologists therefore frequently consider s-ketamine as add-on therapy. This randomised parallel group design trial compares s-ketamine with a placebo as add on medication to a multimodal pain approach., Methods and Analysis: In total 62 adult participants, undergoing ATL for drug resistant epilepsy under general anaesthesia, will be randomised to either receive a 0.25 mg/kg bolus followed by a continuous infusion of 0.1 mg/kg/hour of s-ketamine or placebo (0.9% NaCl) starting before incision and continued for 48 hours as an addition to acetaminophen and opioids administered in a patient-controlled analgesia pump. The primary outcome measure is the cumulative postoperative opioid consumption. Patient recruitment started August 2018 and will end in 2021. Secondary outcome measures are postoperative pain intensity scores, psychological parameters, length of hospital stay and adverse events and will be reassessed at 3 and 6 months after surgery, with a baseline measurement preoperatively. All data are collected by researchers who are blinded to the treatment. The data will be analysed by multivariable linear mixed-effects regression., Ethics and Dissemination: Ethical approval has been given by the local medical ethical committee (NL61666.068.17). This study will be conducted in accordance with the Dutch Medical Research Involving Human Subjects Act and the Declaration of Helsinki. The results of this trial will be publicly disclosed and submitted for publication in an international peer-reviewed scientific journal., Trial Registration Number: NTR6480., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2019
- Full Text
- View/download PDF
36. DNA methyltransferase isoforms expression in the temporal lobe of epilepsy patients with a history of febrile seizures.
- Author
-
de Nijs L, Choe K, Steinbusch H, Schijns OEMG, Dings J, van den Hove DLA, Rutten BPF, and Hoogland G
- Subjects
- Case-Control Studies, Cross-Sectional Studies, DNA Methylation, DNA Methyltransferase 3A, Epigenesis, Genetic, Female, Gene Expression Regulation, Neoplastic, Humans, Male, Organ Specificity, Pilot Projects, Seizures, Febrile genetics, DNA (Cytosine-5-)-Methyltransferase 1 genetics, DNA (Cytosine-5-)-Methyltransferases genetics, Epilepsy, Temporal Lobe genetics, Hippocampus chemistry, Neocortex chemistry, Seizures, Febrile epidemiology
- Abstract
Background: Temporal lobe epilepsy (TLE) with hippocampal sclerosis (HS) is a common pharmaco-resistant epilepsy referred for adult epilepsy surgery. Though associated with prolonged febrile seizures (FS) in childhood, the neurobiological basis for this relationship is not fully understood and currently no preventive or curative therapies are available. DNA methylation, an epigenetic mechanism catalyzed by DNA methyltransferases (DNMTs), potentially plays a pivotal role in epileptogenesis associated with FS. In an attempt to start exploring this notion, the present cross-sectional pilot study investigated whether global DNA methylation levels (5-mC and 5-hmC markers) and DNMT isoforms (DNMT1, DNMT3a1, and DNMT3a2) expression would be different in hippocampal and neocortical tissues between controls and TLE patients with or without a history of FS., Results: We found that global DNA methylation levels and DNMT3a2 isoform expression were lower in the hippocampus for all TLE groups when compared to control patients, with a more significant decrease amongst the TLE groups with a history of FS. Interestingly, we showed that DNMT3a1 expression was severely diminished in the hippocampus of TLE patients with a history of FS in comparison with control and other TLE groups. In the neocortex, we found a higher expression of DNMT1 and DNMT3a1 as well as increased levels of global DNA methylation for all TLE patients compared to controls., Conclusion: Together, the findings of this descriptive cross-sectional pilot study demonstrated brain region-specific changes in DNMT1 and DNMT3a isoform expression as well as global DNA methylation levels in human TLE with or without a history of FS. They highlighted a specific implication of DNMT3a isoforms in TLE after FS. Therefore, longitudinal studies that aim at targeting DNMT3a isoforms to evaluate the potential causal relationship between FS and TLE or treatment of FS-induced epileptogenesis seem warranted.
- Published
- 2019
- Full Text
- View/download PDF
37. Single-Cell Recordings to Target the Anterior Nucleus of the Thalamus in Deep Brain Stimulation for Patients with Refractory Epilepsy.
- Author
-
Schaper FLWVJ, Zhao Y, Janssen MLF, Wagner GL, Colon AJ, Hilkman DMW, Gommer E, Vlooswijk MCG, Hoogland G, Ackermans L, Bour LJ, Van Wezel RJA, Boon P, Temel Y, Heida T, Van Kranen-Mastenbroek VHJM, and Rouhl RPW
- Subjects
- Adult, Aged, Anterior Thalamic Nuclei cytology, Anterior Thalamic Nuclei diagnostic imaging, Deep Brain Stimulation instrumentation, Drug Resistant Epilepsy diagnostic imaging, Drug Resistant Epilepsy physiopathology, Female, Humans, Male, Microelectrodes, Middle Aged, Prospective Studies, Anterior Thalamic Nuclei physiology, Deep Brain Stimulation methods, Drug Resistant Epilepsy therapy, Neurons physiology
- Abstract
Deep brain stimulation (DBS) of the anterior nucleus of the thalamus (ANT) is a promising treatment for patients with refractory epilepsy. However, therapy response varies and precise positioning of the DBS lead is potentially essential for maximizing therapeutic efficacy. We investigate if single-cell recordings acquired by microelectrode recordings can aid targeting of the ANT during surgery and hypothesize that the neuronal firing properties of the target region relate to clinical outcome. We prospectively included 10 refractory epilepsy patients and performed microelectrode recordings under general anesthesia to identify the change in neuronal signals when approaching and transecting the ANT. The neuronal firing properties of the target region, anatomical locations of microelectrode recordings and active contact positions of the DBS lead along the recorded trajectory were compared between responders and nonresponders to DBS. We obtained 19 sets of recordings from 10 patients (five responders and five nonresponders). Amongst the 403 neurons detected, 365 (90.6%) were classified as bursty. Entry into the ANT was characterized by an increase in firing rate while exit of the ANT was characterized by a decrease in firing rate. Comparing the trajectories of responders to nonresponders, we found differences neither in the neuronal firing properties themselves nor in their locations relative to the position of the active contact. Single-cell firing rate acquired by microelectrode recordings under general anesthesia can thus aid targeting of the ANT during surgery, but is not related to clinical outcome in DBS for patients with refractory epilepsy.
- Published
- 2019
- Full Text
- View/download PDF
38. Brain tissue plasticity: protein synthesis rates of the human brain.
- Author
-
Smeets JSJ, Horstman AMH, Schijns OEMG, Dings JTA, Hoogland G, Gijsen AP, Goessens JPB, Bouwman FG, Wodzig WKWH, Mariman EC, and van Loon LJC
- Subjects
- Adult, Brain surgery, Carbon Isotopes, Epilepsy, Temporal Lobe blood, Epilepsy, Temporal Lobe surgery, Female, Humans, Male, Middle Aged, Neuronavigation, Neurosurgical Procedures methods, Phenylalanine metabolism, Time Factors, Brain physiopathology, Epilepsy, Temporal Lobe pathology, Neuronal Plasticity physiology, Proteins metabolism
- Abstract
All tissues undergo continuous reconditioning via the complex orchestration of changes in tissue protein synthesis and breakdown rates. Skeletal muscle tissue has been well studied in this regard, and has been shown to turnover at a rate of 1-2% per day in vivo in humans. Few data are available on protein synthesis rates of other tissues. Because of obvious limitations with regard to brain tissue sampling no study has ever measured brain protein synthesis rates in vivo in humans. Here, we applied stable isotope methodology to directly assess protein synthesis rates in neocortex and hippocampus tissue of six patients undergoing temporal lobectomy for drug-resistant temporal lobe epilepsy (Clinical trial registration: NTR5147). Protein synthesis rates of neocortex and hippocampus tissue averaged 0.17 ± 0.01 and 0.13 ± 0.01%/h, respectively. Brain tissue protein synthesis rates were 3-4-fold higher than skeletal muscle tissue protein synthesis rates (0.05 ± 0.01%/h; P < 0.001). In conclusion, the protein turnover rate of the human brain is much higher than previously assumed.
- Published
- 2018
- Full Text
- View/download PDF
39. Methodology, outcome, safety and in vivo accuracy in traditional frame-based stereoelectroencephalography.
- Author
-
van der Loo LE, Schijns OEMG, Hoogland G, Colon AJ, Wagner GL, Dings JTA, and Kubben PL
- Subjects
- Adolescent, Adult, Drug Resistant Epilepsy diagnosis, Electroencephalography adverse effects, Female, Humans, Male, Middle Aged, Postoperative Complications epidemiology, Postoperative Complications prevention & control, Drug Resistant Epilepsy surgery, Electrodes, Implanted adverse effects, Electroencephalography methods, Postoperative Complications etiology, Stereotaxic Techniques adverse effects
- Abstract
Background: Stereoelectroencephalography (SEEG) is an established diagnostic technique for the localization of the epileptogenic zone in drug-resistant epilepsy. In vivo accuracy of SEEG electrode positioning is of paramount importance since higher accuracy may lead to more precise resective surgery, better seizure outcome and reduction of complications., Objective: To describe experiences with the SEEG technique in our comprehensive epilepsy center, to illustrate surgical methodology, to evaluate in vivo application accuracy and to consider the diagnostic yield of SEEG implantations., Methods: All patients who underwent SEEG implantations between September 2008 and April 2016 were analyzed. Planned electrode trajectories were compared with post-implantation trajectories after fusion of pre- and postoperative imaging. Quantitative analysis of deviation using Euclidean distance and directional errors was performed. Explanatory variables for electrode accuracy were analyzed using linear regression modeling. The surgical methodology, procedure-related complications and diagnostic yield were reported., Results: Seventy-six implantations were performed in 71 patients, and a total of 902 electrodes were implanted. Median entry and target point deviations were 1.54 mm and 2.93 mm. Several factors that predicted entry and target point accuracy were identified. The rate of major complications was 2.6%. SEEG led to surgical therapy of various modalities in 53 patients (69.7%)., Conclusions: This study demonstrated that entry and target point localization errors can be predicted by linear regression models, which can aid in identification of high-risk electrode trajectories and further enhancement of accuracy. SEEG is a reliable technique, as demonstrated by the high accuracy of conventional frame-based implantation methodology and the good diagnostic yield.
- Published
- 2017
- Full Text
- View/download PDF
40. Validation of reference genes in human chordoma.
- Author
-
Santegoeds RGC, Yakkioui Y, Jahanshahi A, Hoogland G, Temel Y, and van Overbeeke JJ
- Abstract
Background: Chordoma are rare slow-growing tumors of the axial skeleton, which are thought to arise from remnants of the notochord. Little is known about the underlying mechanisms that drive this tumor. However, the assessment of gene expression levels by quantitative real-time polymerase chain reaction (qRT-PCR) is hampered due to a lack of validated reference genes. Using an unstable reference gene in qRT-PCR may lead to irreproducible results., Methods: The expression of 12 candidate reference genes ( ACTB , B2M , T , EF1a , GAPDH , HPRT , KRT8 , KRT19 , PGK1 , RS27a , TBP , and YWHAZ ) was analyzed by qRT-PCR in flash frozen chordoma samples from 18 patients. GeNorm and NormFinder algorithms were used to rank the stability of the genes., Results: From most to least stably expressed, the top six genes found by geNorm were PGK1 , YWHAZ , ACTB , HPRT , EF1A , and TBP . When analyzed by NormFinder, the top six genes were ACTB , YWHAZ , PGK1 , B2M , TBP , and HPRT . GAPDH alone, which is often used as a reference gene in chordoma gene expression studies, is not stable enough for reliable results., Conclusion: In gene expression studies of human chordomas, PGK1 , ACTB , and YWHAZ are more stably expressed, and therefore, are preferred reference genes over the most often used reference gene so far, GAPDH ., Competing Interests: There are no conflicts of interest.
- Published
- 2017
- Full Text
- View/download PDF
41. Dystrophin Distribution and Expression in Human and Experimental Temporal Lobe Epilepsy.
- Author
-
Hendriksen RG, Schipper S, Hoogland G, Schijns OE, Dings JT, Aalbers MW, and Vles JS
- Abstract
Objective: Dystrophin is part of a protein complex that connects the cytoskeleton to the extracellular matrix. In addition to its role in muscle tissue, it functions as an anchoring protein within the central nervous system such as in hippocampus and cerebellum. Its presence in the latter regions is illustrated by the cognitive problems seen in Duchenne Muscular Dystrophy (DMD). Since epilepsy is also supposed to constitute a comorbidity of DMD, it is hypothesized that dystrophin plays a role in neuronal excitability. Here, we aimed to study brain dystrophin distribution and expression in both, human and experimental temporal lobe epilepsy (TLE)., Method: Regional and cellular dystrophin distribution was evaluated in both human and rat hippocampi and in rat cerebellar tissue by immunofluorescent colocalization with neuronal (NeuN and calbindin) and glial (GFAP) markers. In addition, hippocampal dystrophin levels were estimated by Western blot analysis in biopsies from TLE patients, post-mortem controls, amygdala kindled (AK)-, and control rats., Results: Dystrophin was expressed in all hippocampal pyramidal subfields and in the molecular-, Purkinje-, and granular cell layer of the cerebellum. In these regions it colocalized with GFAP, suggesting expression in astrocytes such as Bergmann glia (BG) and velate protoplasmic astrocytes. In rat hippocampus and cerebellum there were neither differences in dystrophin positive cell types, nor in the regional dystrophin distribution between AK and control animals. Quantitatively, hippocampal full-length dystrophin (Dp427) levels were about 60% higher in human TLE patients than in post-mortem controls (p < 0.05), whereas the level of the shorter Dp71 isoform did not differ. In contrast, AK animals showed similar dystrophin levels as controls., Conclusion: Dystrophin is ubiquitously expressed by astrocytes in the human and rat hippocampus and in the rat cerebellum. Hippocampal full-length dystrophin (Dp427) levels are upregulated in human TLE, but not in AK rats, possibly indicating a compensatory mechanism in the chronic epileptic human brain.
- Published
- 2016
- Full Text
- View/download PDF
42. Experimental febrile seizures increase dendritic complexity of newborn dentate granule cells.
- Author
-
Raijmakers M, Clynen E, Smisdom N, Nelissen S, Brône B, Rigo JM, Hoogland G, and Swijsen A
- Subjects
- Age Factors, Animals, Animals, Newborn, Calbindin 2 metabolism, Convulsants toxicity, Dentate Gyrus growth & development, Disease Models, Animal, Doublecortin Domain Proteins, Doublecortin Protein, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, HEK293 Cells, Humans, Male, Microtubule-Associated Proteins metabolism, Neurons metabolism, Neurons pathology, Neuropeptides metabolism, Phosphopyruvate Hydratase metabolism, Polymethyl Methacrylate toxicity, Rats, Rats, Sprague-Dawley, Seizures, Febrile chemically induced, Transduction, Genetic, Transfection, Dendrites physiology, Dentate Gyrus pathology, Neurons ultrastructure, Seizures, Febrile pathology
- Abstract
Objective: Febrile seizures (FS) are fever-associated convulsions, being the most common seizure disorder in early childhood. A subgroup of these children later develops epilepsy characterized by a hyperexcitable neuronal network in the hippocampus. Hippocampal excitability is regulated by the hippocampal dentate gyrus (DG) where postnatal neurogenesis occurs. Experimental FS increase the survival of newborn hippocampal dentate granule cells (DGCs), yet the significance of this neuronal subpopulation to the hippocampal network remains unclear. In the current study, we characterized the temporal maturation and structural integration of these post-FS born DGCs in the DG., Methods: Experimental FS were induced in 10-day-old rat pups. The next day, retroviral particles coding for enhanced green fluorescent protein (eGFP) were stereotactically injected in the DG to label newborn cells. Histochemical analyses of eGFP expressing DGCs were performed one, 4, and 8 weeks later and consisted of the following: (1) colocalization with neurodevelopmental markers doublecortin, calretinin, and the mature neuronal marker NeuN; (2) quantification of dendritic complexity; and (3) quantification of spine density and morphology., Results: At neither time point were neurodevelopmental markers differently expressed between FS animals and normothermia (NT) controls. One week after treatment, DGCs from FS animals showed dendrites that were 66% longer than those from NT controls. At 4 and 8 weeks, Sholl analysis of the outer 83% of the molecular layer showed 20-25% more intersections in FS animals than in NT controls (p < 0.01). Although overall spine density was not affected, an increase in mushroom-type spines was observed after 8 weeks., Significance: Experimental FS increase dendritic complexity and the number of mushroom-type spines in post-FS born DGCs, demonstrating a more mature phenotype and suggesting increased incoming excitatory information. The consequences of this hyperconnectivity to signal processing in the DG and the output of the hippocampus remain to be studied., (Wiley Periodicals, Inc. © 2016 International League Against Epilepsy.)
- Published
- 2016
- Full Text
- View/download PDF
43. An Unusual Triad in Pediatric Neurology: A Case Report on Cerebral Palsy, Epilepsy, and Duchenne Muscular Dystrophy.
- Author
-
Hendriksen RGF, Aalbers MW, Hendriksen JGM, de Die-Smulders CEM, Hoogland G, and Vles JSH
- Abstract
We present a case of an unusual triad in pediatric neurology: a currently 12-year-old boy with cerebral palsy and epilepsy who was later also diagnosed with Duchenne muscular dystrophy. We describe the clinical path that resulted in this exceptional diagnosis. This case report illustrates how different neurological disorders may overshadow each other. In addition, it demonstrates that every child with cerebral palsy and either an atypical clinical course or with inexplicable laboratory values-as well as every infant boy born to a theoretical Duchenne muscular dystrophy carrier-should be subjected to additional investigations., Competing Interests: Declaration of Conflicting Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2016
- Full Text
- View/download PDF
44. Long-term drug-resistant temporal lobe epilepsy associated with a mixed ganglioglioma and dysembryoplastic neuroepithelial tumor in an elderly patient.
- Author
-
Schijns OE, Beckervordersandforth J, Wagner L, and Hoogland G
- Abstract
Background: Mixed ganglioglioma and dysembryoplastic neuroepithelial tumor (DNET) is an extremely rare neuropathological diagnosis. The sparse number of patients described are children or young adults with long-term drug-resistant epilepsy., Case Description: We report on a rare case of this tumor in a 61-year-old patient with an epilepsy duration of almost 60 years. This patient received an epilepsy surgery work-up with the intention to cure his drug-resistant epilepsy by performing a complete lesionectomy. The available literature on these mixed tumors is reviewed., Conclusion: A contrast-enhancing mixed ganglioglioma and DNET can mimic a malignant tumor and appears not only in children and young adults, but also in the elderly patients with chronic epilepsy. A long-lasting epilepsy, in this case almost 60 years, can be completely cured by a complete lesionectomy.
- Published
- 2016
- Full Text
- View/download PDF
45. Sustained Reduction of Cerebellar Activity in Experimental Epilepsy.
- Author
-
Rijkers K, Moers-Hornikx VM, Hemmes RJ, Aalbers MW, Temel Y, Vles JS, and Hoogland G
- Subjects
- Animals, Epilepsy metabolism, Immunohistochemistry, Male, Proto-Oncogene Proteins c-fos chemistry, Proto-Oncogene Proteins c-fos metabolism, Rats, Rats, Sprague-Dawley, Cerebellum chemistry, Cerebellum physiopathology, Epilepsy physiopathology
- Abstract
Clinical and experimental evidence suggests a role for the cerebellum in seizure control, while no data are available on cerebellar activity between seizures. We hypothesized that interictal regional activity of the deep cerebellar nuclei is reduced in epilepsy and tested this in an animal model by using ΔFosB and cytochrome oxidase (COX) (immuno)histochemistry. The expression of these two markers of neuronal activity was analysed in the dentate nucleus (DN), interpositus nucleus (IN), and fastigial nucleus (FN) of the cerebellum of fully amygdala kindled rats that were sacrificed 48 hours after their last seizure. The DN and FN of kindled rats exhibited 25 to 29% less ΔFosB immunopositive cells than their respective counterpart in sham controls (P < 0.05). COX expression in the DN and FN of kindled animals was reduced by 32 to 33% compared to respective control values (P < 0.05). These results indicate that an epileptogenic state is characterized by decreased activity of deep cerebellar nuclei, especially the DN and FN. Possible consequences may include a decreased activation of the thalamus, contributing to further seizure spread. Restoration of FN activity by low frequency electrical stimulation is suggested as a possible treatment option in chronic epilepsy.
- Published
- 2015
- Full Text
- View/download PDF
46. Elevated IL-1β and IL-6 levels in lumbar herniated discs in patients with sciatic pain.
- Author
-
Andrade P, Hoogland G, Garcia MA, Steinbusch HW, Daemen MA, and Visser-Vandewalle V
- Subjects
- Adult, Biomarkers metabolism, Biopsy, Case-Control Studies, Female, Humans, Intervertebral Disc Displacement pathology, Male, Pain Measurement, RNA, Messenger metabolism, Sciatica pathology, Scoliosis metabolism, Scoliosis pathology, Treatment Outcome, Diskectomy, Interleukin-1beta metabolism, Interleukin-6 metabolism, Intervertebral Disc Displacement metabolism, Intervertebral Disc Displacement surgery, Lumbar Vertebrae, Sciatica metabolism
- Abstract
Purpose: Previous experimental models have shown that proinflammatory cytokines modulate peripheral and central nociception. However, the direct correlation between inflammation and pain in patients remains unclear. Our aim is to correlate the levels of inflammation in the spine with pre- and postoperative pain scores after discectomy., Methods: Paravertebral muscle, annulus fibrosus (AF) and nucleus pulposus (NP) biopsies were intraoperatively collected from ten lumbar disc hernia (LDH) patients suffering from chronic sciatic pain and, as painless controls, five scoliosis patients. IL-1β and IL-6 expressions in these biopsies were assessed by qPCR and western blot. The amount of pain, indicated on a 0-10 point visual analogue scale (VAS), was assessed 1 day before surgery and 6 weeks and 1 year after surgery. For analysis purposes, LDH patients were grouped into painful (VAS ≥ 3.5) and non-painful (VAS < 3.5). LDH painful patient group showed a onefold increased mRNA expression of IL-1β in the NP, and IL-6 in the AF and NP (p < 0.05 vs. controls)., Results: By western blot analysis, both cytokines were clearly visible in all LDH biopsies, but not in controls. However, cytokine expression of the painful patient group did not differ from those of the non-painful patient group. In addition, there was no correlation between VAS scores and either marker., Conclusions: These findings support the idea that LDH is accompanied by a local inflammatory process. Yet, the lack of correlation between IL-1β or IL-6 expression and the severity pain suggests that these cytokines may not play a leading role in maintaining a pain generating network.
- Published
- 2013
- Full Text
- View/download PDF
47. Validation of reference genes for quantitative real-time PCR studies in the dentate gyrus after experimental febrile seizures.
- Author
-
Swijsen A, Nelissen K, Janssen D, Rigo JM, and Hoogland G
- Subjects
- Air, Algorithms, Animals, Animals, Newborn, Gene Expression, Gene Expression Profiling, Genes, Essential, Hot Temperature, Male, Rats, Rats, Sprague-Dawley, Real-Time Polymerase Chain Reaction standards, Reference Standards, Cyclophilin A genetics, Dentate Gyrus metabolism, Nerve Tissue Proteins genetics, Ribosomal Proteins genetics, Seizures, Febrile genetics, Software, TATA-Box Binding Protein genetics
- Abstract
Background: Quantitative real-time PCR (qPCR) is a commonly used technique to quantify gene expression levels. Validated normalization is essential to obtain reliable qPCR data. In that context, normalizing to multiple reference genes has become the most popular method. However, expression of reference genes may vary per tissue type, developmental stage and in response to experimental treatment. It is therefore imperative to determine stable reference genes for a specific sample set and experimental model. The present study was designed to validate potential reference genes in hippocampal tissue from rats that had experienced early-life febrile seizures (FS). To this end, we applied an established model in which FS were evoked by exposing 10-day old rat pups to heated air. One week later, we determined the expression stability of seven frequently used reference genes in the hippocampal dentate gyrus., Results: Gene expression stability of 18S rRNA, ActB, GusB, Arbp, Tbp, CycA and Rpl13A was tested using geNorm and Normfinder software. The ranking order of reference genes proposed by geNorm was not identical to that suggested by Normfinder. However, both algorithms indicated CycA, Rpl13A and Tbp as the most stable genes, whereas 18S rRNA and ActB were found to be the least stably expressed genes., Conclusions: Our data demonstrate that the geometric averaging of at least CycA, Rpl13A and Tbp allows reliable interpretation of gene expression data in this experimental set-up. The results also show that ActB and 18S rRNA are not suited as reference genes in this model.
- Published
- 2012
- Full Text
- View/download PDF
48. Experimental early-life febrile seizures induce changes in GABA(A) R-mediated neurotransmission in the dentate gyrus.
- Author
-
Swijsen A, Avila A, Brône B, Janssen D, Hoogland G, and Rigo JM
- Subjects
- Age Factors, Animals, Inhibitory Postsynaptic Potentials physiology, Male, Rats, Rats, Sprague-Dawley, gamma-Aminobutyric Acid physiology, Dentate Gyrus physiology, Receptors, GABA-A physiology, Seizures, Febrile physiopathology, Synaptic Transmission physiology
- Abstract
Purpose: Febrile seizures (FS), the most frequent seizure type during childhood, have been linked to temporal lobe epilepsy (TLE) in adulthood. Yet, underlying mechanisms are still largely unknown. Altered γ-aminobutyric acid (GABA)ergic neurotransmission in the dentate gyrus (DG) circuit has been hypothesized to be involved. This study aims at analyzing whether experimental FS change inhibitory synaptic input and postsynaptic GABA(A) R function in dentate granule cells., Methods: We applied an immature rat model of hyperthermia (HT)-induced FS. GABA(A) R-mediated neurotransmission was studied using whole-cell patch-clamp recordings from dentate granule neurons in hippocampal slices within 6-9 days post-HT., Key Findings: Frequencies of spontaneous inhibitory postsynaptic currents (sIPSCs) were reduced in HT rats that had experienced seizures, whereas sIPSC amplitudes were enhanced. Whole-cell GABA responses revealed a doubled GABA(A) R sensitivity in dentate granule cells from HT animals, compared to that of normothermic (NT) controls. Analysis of sIPSCs and whole-cell GABA responses showed similar kinetics in postsynaptic GABA(A) Rs of HT and NT rats. quantitative real-time polymerase chain reaction (qPCR) experiments indicated changes in DG GABA(A) R subunit expression, which was most pronounced for the α3 subunit., Significance: The data support the hypothesis that FS persistently alter neuronal excitability., (Wiley Periodicals, Inc. © 2012 International League Against Epilepsy.)
- Published
- 2012
- Full Text
- View/download PDF
49. Cytogenesis in the dentate gyrus after neonatal hyperthermia-induced seizures: what becomes of surviving cells?
- Author
-
Lemmens EM, Schijns OE, Beuls EA, and Hoogland G
- Subjects
- Amino Acid Transport System X-AG metabolism, Amino Acid Transport System X-AG physiology, Animals, Animals, Newborn growth & development, Antigens, Nuclear, Bromodeoxyuridine pharmacology, Cell Count, Cell Division physiology, Excitatory Amino Acid Transporter 3 metabolism, Fluorescent Antibody Technique, Glial Fibrillary Acidic Protein, Glutamate Decarboxylase, Hippocampus cytology, Hippocampus metabolism, Hippocampus physiopathology, Microglia metabolism, Nerve Tissue Proteins drug effects, Neurons drug effects, Neurons physiology, Plant Lectins, Rats, Rats, Sprague-Dawley, Seizures, Febrile metabolism, Cell Proliferation, Cell Survival physiology, Dentate Gyrus cytology, Fever complications, Seizures, Febrile etiology, Seizures, Febrile pathology
- Abstract
Purpose: Febrile seizures (FS) are early-life seizures thought to play a role in epileptogenesis. By labeling cells that were dividing immediately following experimental FS, we previously demonstrated that significantly more of these newborn cells in the dentate gyrus (DG) survived 8 weeks later, relative to animals that did not experience FS. The purpose of the present study was to determine the long-term fate of these newborn cells., Methods: On postnatal day (PN) 10, hyperthermia-induced seizures (HT, +/-42 degrees C core temperature) were evoked in Sprague-Dawley rats and littermates were used as normothermia controls (NT, +/-35 degrees C core temperature). From PN11 to PN16, rats were injected with bromodeoxyuridine (BrdU) to label dividing cells. At PN66, we evaluated the number of BrdU-labeled cells in the DG that colocalized with the neuronal marker NeuN, glial marker glial fibrillary acidic protein (GFAP), neuronal excitatory amino acid transporter 3 (EAAT3), GABAergic neuronal marker glutamic acid decarboxylase 67 (GAD67) or microglia marker tomato lectin (TL)., Results: In all rats, almost all BrdU-labeled cells in the DG, that showed double-labeling, colocalized with NeuN, and rarely with GFAP, GAD67, or TL. In NT controls and HT rats that did not experience seizures ("HT-no seizures"), approximately 23% of BrdU-labeled cells colocalized with EAAT3, which was significantly different from 14% in HT rats that did experience seizures (HT + FS)., Discussion: Early-life seizures decrease the population of newborn cells that survive and mature into EAAT3-positive neurons and do not affect the GABAergic cell population. This may affect hippocampal physiology in young adulthood.
- Published
- 2008
- Full Text
- View/download PDF
50. Gender differences in febrile seizure-induced proliferation and survival in the rat dentate gyrus.
- Author
-
Lemmens EM, Lubbers T, Schijns OE, Beuls EA, and Hoogland G
- Subjects
- Animals, Animals, Newborn, Body Temperature, Bromodeoxyuridine, Cell Count, Cell Survival, Dentate Gyrus cytology, Dentate Gyrus metabolism, Disease Models, Animal, Female, Fever pathology, Hot Temperature, Male, Neurons metabolism, Rats, Rats, Sprague-Dawley, Seizures, Febrile diagnosis, Seizures, Febrile etiology, Sex Factors, Cell Proliferation, Dentate Gyrus pathology, Neurons pathology, Seizures, Febrile pathology
- Abstract
Purpose: Febrile seizures are fever-associated early-life seizures that are thought play a role in the development of epilepsy. Seizure-induced proliferation of dentate granule cells has been demonstrated in several adult animal models and is thought to be an integral part of epileptogenesis. The aim of the present study was to investigate proliferation and survival of dentate gyrus (DG) cells born after early-life hyperthermia (HT)-induced seizures in male and female rats., Methods: At postnatal day (PN) 10, male and female rats were exposed to heated air to induce seizures. Littermates were used as normothermia controls. Convulsive behavior was observed by two researchers. From PN11 to PN16, rats were injected with bromodeoxyuridine (BrdU) to label dividing cells. The number of BrdU-immunoreactive cells in the DG was counted at PN17 and PN66., Results: At PN17, male as well as female HT rats had the same amount of BrdU-positive cells compared with controls. At PN66, significantly more BrdU-positive cells were left in HT females (53%) than in controls (44%, percentage of BrdU-positive cells at PN17), whereas no difference was found between HT males and male controls. The net result of proliferation and survival at PN66 was that female HT rats had the same number of BrdU-immunoreactive cells as controls, whereas male HT rats had 25% more BrdU-immunoreactive cells than did controls (p < 0.05)., Conclusions: Early-life seizures cause a sexually dimorphic cytogenic response that results in an increased population of newborn DG cells in young adult males, while leaving that of young adult females unaltered.
- Published
- 2005
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.