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The molecular basis for leaf development, a major focus in developmental biology, remains unclear in the monocotyledonous grass, rice (Oryza sativa). Here, we performed a mutant screen in rice and identified an AP2-type transcription factor family protein, NARROW AND DWARF LEAF1 (NDL1). NDL1 is the ortholog of Arabidopsis thaliana (subsequently called Arabidopsis) ENHANCER OF SHOOT REGENERATION1 (ESR1)/DORNRÖSCHEN (DRN) and mediates leaf development and maintenance of the shoot apical meristem (SAM). Loss of function of NDL1 results in bladeless leaves and SAMs that are flat, rather than dome-shaped, and lack cell proliferation activity. This loss of function also causes reduced auxin signaling. Moreover, as is the case with Arabidopsis ESR1/DRN, NDL1 plays crucial roles in shoot regeneration. Importantly, we found that NDL1 is not expressed in the SAM but is expressed in leaf primordia. We propose that NDL1 cell autonomously regulates leaf development, but non-cell autonomously regulates SAM maintenance in rice. [ABSTRACT FROM AUTHOR]

This article proposes a novel continuous pulsewidth modulation (CPWM) method to reduce dc-link current harmonics in voltage source inverters over wide range of load power factor. This modulation method contributes to a current stress reduction of dc-link smoothing capacitor and a suppression of its temperature rise. Furthermore, high-cost digital hardware, such as a field-programmable gate array, is not necessary because this modulation is implemented with only one carrier. The dc-link current harmonics are reduced by shifting voltage references in every half control period to reduce a fluctuation of the dc-link current around its average value. Experimental results confirm that the application of the proposed CPWM reduces the dc-link current harmonics by 24.2% at most and lowers an equilibrium capacitor temperature by 6.0 °C compared to the conventional CPWM. [ABSTRACT FROM AUTHOR]

This paper proposes a control for a single-phase grid-tied inverter operating in both continuous current mode (CCM) and discontinuous current mode (DCM) to minimize inductors without worsening a current total harmonic distortion (THD). In a conventional CCM/DCM control, an inductance is required in a DCM nonlinearity compensation; consequently, the control becomes inductance-dependent. In the proposed control, a duty ratio at a previous calculation period is utilized to compensate for the DCM nonlinearity and detect current modes independently from the inductance. A 4-kW 100-kHz prototype of the inverter with two designs of the inductor is realized to confirm the effectiveness of the proposed control. When the inductor impedance, which is normalized by an inverter impedance, is reduced from 1.8% to 0.5%, volume and material cost of the inductor are reduced by 51% and 62%, respectively, whereas the loss at a light load of 0.1 p.u. is reduced by 35%. However, due to this inductor minimization, the current THD at a rated load increases from 2.3% to 8.7% with the conventional control, violating the grid current harmonic constraint regulated by standard IEEE-1547. The proposed CCM/DCM control reduces the current THD from 8.7% to 2.1%, enabling the inductor minimization and satisfies the grid standard. [ABSTRACT FROM AUTHOR]

This paper proposes a novel space vector pulsewidth modulation (SVPWM) strategy to reduce the switching-frequency-order inverter input current harmonics for a two-level three-phase voltage-source inverter operating over a wide range of the load power factor. The fluctuation of the inverter input current around its average value is minimized by optimization of the utilized voltage space vectors to reduce the inverter input current harmonics, which extends the lifetime of the dc-link electrolytic capacitors. Furthermore, the proposed strategy can be adapted to a motor drive system, where a wide variation of the load power factor is desired, by changing the optimized combinations of the voltage space vectors according to the polarities of the output phase currents. Experiments with the application of the novel SVPWM confirm that the inverter input current harmonics is reduced by up to 29.5% compared to that with the conventional SVPWM strategy. Moreover, the analytical and experimental results confirm that the proposed SVPWM reduces the inverter input current harmonics in both driving mode and regenerative braking mode, as well as with any load power factor. [ABSTRACT FROM AUTHOR]

This paper presents a high power density design for a step-down rectifier incorporating a modular multilevel converter (MMC) in a power system connected to a 6.6 kV ac grid. In particular, the relationship among the number of cells, the output voltage of the MMC, and the overall volume is clarified. A proposed design flowchart focuses on minimizing volumes of cell capacitors, heat sinks, and arm inductors by using optimal number of cells. Moreover, the commercial electrolytic capacitors are used as the cell capacitor. Besides, the formulae used to determine the ripple currents in the electrolytic capacitor and the arm inductor are presented, along with that for the semiconductor loss. Each of these formulae were verified experimentally using a miniature model and theoretical values from all formulae agree with the measured values within minimal deviations that are discussed with regard to the design of a practical converter for a 6.6 kV system. Finally, the conditions necessary to achieve high power density in an MMC are provided, based on volume evaluations of the electrolytic capacitor, the arm inductor, and the heat sink. Using this optimization process, an MMC design achieved a volume reduction of approximately 90% compared to a conventional system. [ABSTRACT FROM PUBLISHER]

This paper proposes a hybrid current mode between triangular current mode (TCM) and discontinuous current mode (DCM) in order to improve a wide-load-range efficiency for a high-frequency SiC-based boost converter. At rated load, TCM, where the zero-voltage switching (ZVS) is achieved, is used in order to increase the switching frequency and minimize the boost converter. At light load, the hybrid discontinuous current mode (HDCM), where the TCM current is flown during the zero-current interval of DCM, is applied in order to achieve both ZVS and a reduction of a current ripple. This maintains a high efficiency over a wide load range. A 1-kW prototype is realized to compare HDCM over continuous current mode (CCM), DCM, and TCM. Compared to TCM, the root-mean-square current is reduced up to 56.6% with HDCM. Consequently, the efficiency of HDCM at light load of 0.1 p.u. is improved by 3.1%. Compared to DCM, the average-current ripple in HDCM is reduced by 82.3%, whereas the efficiency of HDCM at light load of 0.1 p.u. is improved by 1.5%. Finally, when the current ripple of CCM is designed to be half of TCM, the efficiency of HDCM at rated load is improved by 0.9% compared to CCM. [ABSTRACT FROM AUTHOR]

This paper presents a matrix converter design for achieving maximum power density using a SiC device based on a front-loading design. To design the matrix converter to achieve maximum power density, the conduction loss and the switching loss of the matrix converter are theoretically derived and validated by simulation and experiment. Based on these formulas, the relationship between the efficiency and power density are revealed by using a Pareto–Front curve in order to solve the tradeoff problem between the power density and the efficiency. Moreover, in order to promote the widespread use of the matrix converter instead of a BTB system, it is quantitatively evaluated that the power density in the matrix converter is increased by 4.19 kW/dm3 in comparison to the BTB system. Moreover, the power density of the matrix converter that uses a SiC-MOSFET (ROHM) as the switching device with natural air cooling is 95.0% (2.1 kW/dm3) of the calculated maximum power density. Thus, the power density of the matrix converter is improved by 57.5% by the maximum power density design method. Based on the results, the design method for a high power density ac–ac direct converter is established according to the requisite specifications. [ABSTRACT FROM PUBLISHER]

Background: The rice PLASTOCHRON ( PLA) genes PLA1 and PLA2 regulate leaf maturation and the temporal pattern of leaf initiation. Although the function of PLA genes in the leaf initiation process has been analyzed, little is known about how they affect leaf growth. Previously, we suggested that PLA1 and PLA2 function downstream of the gibberellin (GA) signal transduction pathway. In the present study, we examined the phenotype of a double mutant of pla and slender rice 1 ( slr1), which is a constitutive GA response mutant. By analyzing these double mutants, we discuss the relationship between PLA-related and GA-dependent pathways and the possible function of PLA genes in leaf growth. Findings: Single slr1 and pla mutants exhibited elongated and dwarf phenotypes in the vegetative stage, respectively. The stature and leaf size of the pla1/slr1 and pla2/slr1 double mutants were intermediate between those of the pla and slr1 single mutants. However, the effects of slr1 on leaf elongation were markedly suppressed in the pla1 and pla2 mutant backgrounds. On the other hand, the change in cell length in the double mutants was almost the same as that in the single mutants. An expression analysis of genes involved in GA biosynthesis and catabolism indicated that feedback regulation functioned normally in the pla/slr1 double mutants. Conclusions: Our genetic results confirm that PLA genes regulate leaf growth downstream of the GA pathway. Our findings also suggest that PLA1 and PLA2 are partly required for GA-dependent leaf elongation, mainly by affecting cellular proliferation. [ABSTRACT FROM AUTHOR]

This paper discusses a new circuit configuration and a new control method for a single-phase ac–dc converter with power factor (P.F.) correction and a power pulsation decoupling function. The proposed converter can achieve low total harmonic distortion (THD) on the input current and the power pulsation decoupling function between the input and output sides, which allows low output voltage ripple even on a small output energy buffer at the same time using an active buffer. Therefore, the proposed converter does not require large smoothing capacitors or large smoothing inductors. The buffering energy is stored by a small capacitor, which controls the variation of the capacitor voltage through the active buffer. In this paper, the fundamental operations of the proposed converter are investigated experimentally. The experimental results reveal that the input current THD is 1.44%, the rate of the output voltage ripple is 6.33%, and the input P.F. is over 99%. In addition, a maximum efficiency of over 96% is obtained for a 750-W prototype converter. [ABSTRACT FROM PUBLISHER]

Control strategy and loss evaluation is discussed for a proposed dc/ac two-stage boost converter using the neutral point of a motor. The proposed converter consists of a small boost chopper and a three-phase inverter, which has a boost-up function from the use of the leakage inductance of a motor, instead of a boost-up reactor, for dc to ac conversion. A six-step operation strategy is proposed for the circuit. When the inverter part outputs a square waveform of 180^\circ, the input current has a distortion because the neutral point voltage of the motor fluctuates at a frequency three times the output frequency. The input current is also shown distorted due to the neutral point voltage fluctuation. Feedforward compensation is proposed to reduce the input current fluctuation. In addition, the dc current in the proposed circuit is imposed into the phase current of the motor. Loss evaluation is therefore implemented to investigate the influence of the imposed dc current on the experimental results. Also, finite-element method was used to analyze the motor loss for a 1.5-kW interior-permanent-magnet motor. [ABSTRACT FROM PUBLISHER]

In this paper, a beatless control that enables unity input power factor for the single-pulse operation in the matrix converter is proposed. The proposed method controls the output voltage to synchronize with the output current in terms of voltage–time product during the high output frequency operation. Following the proposed synchronous control method, the beat current is suppressed by approximately 1% at output frequency 1432 Hz. The experimental results also demonstrate that the input current waveform achieves sinusoidal waveform with unity power factor. In addition, the voltage transfer ratio of the matrix converter is improved from 0.866 to 0.955 in the high output frequency region. The maximum efficiency 96.4% is achieved at a 1.34-kW RL load. [ABSTRACT FROM AUTHOR]

This paper discusses two overmodulation controls, namely, square wave and trapezoidal, in the indirect matrix converter (IMC) for motor drive application. The differences between these two modulations are analyzed based on the output–input voltage transfer ratio (VTR), the efficiency, and the current total harmonic distortions under two load conditions. Moreover, the performance is then improved by implementing four-step commutation methods in the primary side of the IMC. The validity of the proposed methods will be demonstrated through simulation and experimentally. The experimental results show that the VTR can be improved from 0.866 to 0.97 in the square wave modulation and 0.92 for the trapezoidal wave modulation in an induction motor drive system. [ABSTRACT FROM PUBLISHER]

Phyllotaxy is defined as the spatial arrangement of leaves on the stem. The mechanism responsible for this extremely regular pattern is one of the most fascinating enigmas in plant biology. In this study, we identified a gene regulating the phyllotactic pattern in rice. Loss-of-function mutants of the DECUSSATE [DEO gene displayed a phyllotactic conversion from normal distichous pattern to decussate. The dec mutants had an enlarged shoot apical meristem with enhanced cell division activity. In contrast to the shoot apical meristem, the size of the root apical meristem in the dec mutants was reduced, and cell division activity was suppressed. These phenotypes indicate that DEC has opposite functions in the shoot apical meristem and root apical meristem. Map-based cloning revealed that DEC encodes a plant-specific protein containing a glutamine-rich region and a conserved motif. Although its molecular function is unclear, the conserved domain is shared with fungi and animals. Expression analysis showed that several type A response regulator genes that act in the cytokinin signaling pathway were down-regulated in the dec mutant. In addition, dec seedlings showed a reduced responsiveness to exogenous cytokinin. Our results suggest that DEC controls the phyllotactic pattern by affecting cytokinin signaling in rice. [ABSTRACT FROM AUTHOR]

RNA silencing is a defense system against "genomic parasites" such as transposable elements (TE), which are potentially harmful to host genomes. In plants, transcripts from TEs induce production of double-stranded RNAs (dsRNAs) and are processed into small RNAs (small interfering RNAs, siRNAs) that suppress TEs by RNA-directed DNA methylation. Thus, the majority of TEs are epigenetically silenced. On the other hand, most of the eukaryotic genome is composed of TEs and their remnants, suggesting that TEs have evolved countermeasures against host-mediated silencing. Under some circumstances, TEs can become active and increase in copy number. Knowledge is accumulating on the mechanisms of TE silencing by the host; however, the mechanisms by which TEs counteract silencing are poorly understood. Here, we show that a class of TEs in rice produces a microRNA (miRNA) to suppress host silencing. Members of the microRNA820 (miR820) gene family are located within CACTA DNA transposons in rice and target a de novo DNA methyltransferase gene, OsDRM2, one of the components of epigenetic silencing. We confirmed that miR820 negatively regulates the expression of OsDRM2. In addition, we found that expression levels of various TEs are increased quite sensitively in response to decreased OsDRM2 expression and DNA methylation at TE loci. Furthermore, we found that the nucleotide sequence of miR820 and its recognition site within the target gene in some Oryza species have co-evolved to maintain their base-pairing ability. The co-evolution of these sequences provides evidence for the functionality of this regulation. Our results demonstrate how parasitic elements in the genome escape the host's defense machinery. Furthermore, our analysis of the regulation of OsDRM2 by miR820 sheds light on the action of transposon-derived small RNAs, not only as a defense mechanism for host genomes but also as a regulator of interactions between hosts and their parasitic elements. [ABSTRACT FROM AUTHOR]

This paper proposes a new circuit topology for a multilevel pulse width modulation (PWM) rectifier. The proposed method forms a new circuit by combining a diode clamp-type topology with a flying capacitor-type topology. The proposed circuit uses only 12 switches, despite the use of a five-level three-phase PWM rectifier. Further, the proposed circuit can obtain good performance, same as a conventional multilevel circuit. This paper describes about the features of the proposed topology; the control strategy and the loss analysis that is estimated by a circuit simulator. In addition, the basic operation of the proposed method is confirmed by simulation and experimental results. The proposed converter achieved total harmonic distortion of 3.4% for the input current and efficiency of 97.4% for a 1 kW class experimental setup. [ABSTRACT FROM AUTHOR]

This paper proposes a square-wave control strategy for a single-phase power factor correction with a new boost-up converter which uses the leakage inductance of a motor instead of a boost-up reactor. Since the power supply is connected to the neutral point of the motor, the current distortion in the power supply occurs when the inverter outputs square waveforms. First, this paper describes the characteristic of the proposed circuit and the problems in the square-wave operation. Next, a current control method for the square waveform is proposed to suppress the current distortion. Finally, the validity of the proposed converter and its control strategy are demonstrated by experimental results. [ABSTRACT FROM PUBLISHER]

Summary Leaf veins have a complex network pattern. Formation of this vein pattern has been widely studied as a model of tissue pattern formation in plants. To understand the molecular mechanism governing the vascular patterning process, we isolated the rice mutant, commissural vein excessive1 ( coe1). The coe1 mutants had short commissural vein (CV) intervals and produced clustered CVs. Application of 1- N-naphthylphthalamic acid and brefeldin A decreased CV intervals, and application of 1-naphthaleneacetic acid increased CV intervals in wild-type rice; however, coe1 mutants were insensitive to these chemicals. COE1 encodes a leucine-rich repeat receptor-like kinase, whose amino acid sequence is similar to that of brassinosteroid-insensitive 1-associated receptor kinase 1 (BAK1), and which is localized at the plasma membrane. Because of the sequence similarity of COE1 to BAK1, we also examined the involvement of brassinosteroids in CV formation. Brassinolide, an active brassinosteroid, decreased the CV intervals of wild-type rice, and brassinazole, an inhibitor of brassinosteroid biosynthesis, increased the CV intervals of wild-type rice, but coe1 mutants showed insensitivity to these chemicals. These results suggest that auxin and brassinosteroids regulate CV intervals in opposite directions, and COE1 may regulate CV intervals downstream of auxin and brassinosteroid signals. [ABSTRACT FROM AUTHOR]

A new bidirectional switch and snubber circuit are proposed for medium-voltage ac/ac converters. The proposed switch can be constructed using 2-in-1 insulated-gate bipolar transistor and 2-in-1 diode modules, and can reduce the voltage stress of the switching device by series connection. The proposed snubber configuration is very simple and can regenerate absorbed energy. On the other hand, timing errors in the switching between the series-connected switches cause a voltage imbalance in the snubber circuit. Therefore, a simple method is proposed for reducing the voltage imbalance that uses one voltage sensor for each switch circuit. This proposed method controls the snubber voltages by adjusting the switch timings. Furthermore, application of the proposed switch circuit to a matrix converter is discussed and is confirmed by experiment. [ABSTRACT FROM AUTHOR]

Members of the Class III homeodomairi leucine zipper (Class III HD-Zip) gene family are central regulators of crucial aspects of plant development. To better understand the roles of five Class III HD-Zip genes in rice (Oryza sativa) development, we investigated their expression patterns, ectopic expression phenotypes, and auxin responsiveness. Four genes, OSHB1 to OSHB4, were expressed in a localized domain of the shoot apical meristem (SAM), the adaxial cells of leaf primordia, the leaf margins, and the xylem tissue of vascular bundles. in contrast, expression of OSHB5 was observed only in phloem tissue. Plants ectopically expressing microRNA166-resistant versions of the OSHB3 gene exhibited severe defects, including the ectopic production of leaf margins, shoots, and radialized leaves. The treatment of seedlings with auxin quickly induced ectopic OSHB3 expression in the entire region of the SAM, but not in other tissues. Furthermore, this ectopic expression of OSHB3 was correlated with leaf initiation defects. Our findings suggest that rice Class III HD-Zip genes have conserved functions with their homologs in Arabidopsis (Arabidopsis thaliana), but have also acquired specific developmental roles in grasses or monocots. In addition, some Class III HD- Zip genes may regulate the leaf initiation process in the SAM in an auxin-dependent manner. [ABSTRACT FROM AUTHOR]

This paper proposes a novel circuit topology for a three-phase power factor correction (PFC) rectifier using a harmonics current injection method. In consideration of lower cost, the harmonics injection method is more suitable than a conventional six-arm pulse width modulation (PWM) rectifier. The harmonics injection current is simply generated by only one switching leg. As a result, the proposed circuit has the advantage of only two switches. An optimal injection current is achieved in order to obtain an input current of sinusoidal waveforms. This paper discusses the basic operation and optimal design method for the proposed circuit. In addition, the validity of the proposed circuit is confirmed by simulation and experimental results. An input current of almost sinusoidal waveform was obtained and an input current total harmonic distortion (THD) of 8.5% was obtained at a load of approximately 1 kW. [ABSTRACT FROM AUTHOR]

Cleistogamy is an efficient strategy for preventing gene flow from genetically modified (GM) crops. We identified a cleistogamous mutant of rice harbouring a missense mutation (the 45th residue isoleucine to threonine; I45T) in the class-B MADS-box gene SUPERWOMAN1 ( SPW1), which specifies the identities of lodicules (equivalent to petals) and stamens. In the mutant, spw1-cls, the stamens are normal, but the lodicules are transformed homeotically to lodicule–glume mosaic organs, thereby engendering cleistogamy. Since this mutation does not affect other agronomic traits, it can be used in crosses to produce transgenic lines that do not cause environmental perturbation. Molecular analysis revealed that the reduced heterodimerization ability of SPW1I45T with its counterpart class-B proteins OsMADS2 and OsMADS4 caused altered lodicule identity. spw1-cls is the first useful mutant for practical gene containment in GM rice. Cleistogamy is possible in many cereals by engineering class-B floral homeotic genes and thereby inducing lodicule identity changes. [ABSTRACT FROM AUTHOR]

The shoot apical meristem (SAM) is a group of stem cells that are responsible for plant development. Mutations in rice SHOOTLESS2 (SHL2), SHL4/SHOOT ORGANIZATION2 (SHO2), and SHO1 cause complete deletion or abnormal formation of the SAM. In this study we showed that defects in SAM formation in shl mutants are associated with the loss of expression of the homeodomain-leucine zipper (HD-ZIPIII) family genes. Rice SHL2, SHL4/SHO2, and SHO1 encoded orthologues of Arabidopsis RNA-dependent RNA polymerase 6, ARGONAUTE (AGO) 7, and DICER-like 4, respectively, whose mutations affect leaf development through the trans-acting siRNA (ta·siRNA) pathway. This suggested that the ta-siRNA pathway regulates the critical step of SAM formation during rice embryogenesis. The gain-of-function experiment by the ectopic expression of SHL4 resulted in reduced accumulation of an microRNA. miR166, and partial adaxialization of leaves, supporting a role for the ta-siRNA pathway in the maintenance of leaf polarity as previously reported in maize. Analysis of the spatiotemporal expression patterns of HD-ZIPIII and miR166 in wild-type and sh mutant embryos suggested that the loss of HD-ZIPIII expression in the SAM region of the developing embryo is the result of ectopic expression of miR166. Our analysis of shl mutants demonstrated that HD-ZIPIII expression regulated by miR166 is sensitive to the ta-siRNA pathway during SAM formation in rice embryogenesis. [ABSTRACT FROM AUTHOR]

Abstract: Since a tokamak fusion reactor will require a fast response and precise power supply to control the coil current, we propose a matrix converter configuration, as a candidate, having a filter in a load side and smaller elements than a conventional matrix converter. Coil current control performance of the candidate converter has been evaluated by a simulation model using the code “PSCAD/EMTDC.” Simulated circuit parameters were: 30mH for the load coil, 1kV for the matrix converter input voltage, and ∼100A for the output coil current. The PWM (pulse width modulation) control using triangular carrier modulation of a 10kHz carrier frequency was adopted as the control method. The simulation results show that the settling delay time of coil current in the step variation is ∼1ms. The transient response performance of the simulated coil current to the reference current was good. We have come to a condition that the matrix converter can be applied to the plasma control coil power supply. [Copyright &y& Elsevier]

While the matrix converter has many advantages that include bidirectional power flow, a size reduction, a long lifetime, and sinusoidal input currents, it is vulnerable to the input voltage disturbances, because it directly exchanges the input voltage to the output voltage. The instantaneous effective power control (IEPC) method has been proposed to compensate the input voltage disturbances, in which the instantaneous effective power is kept constant by controlling input current. However, to date, no method has been proposed to maintain the stability of the system with the IEPC. The purpose of this paper is to propose a method to control the stability of the system with IEPC. First, this paper explains the IEPC theoretically. Next, a model of the system with the IEPC is developed, and stability analyses are conducted. Then, based on the results of the analyses, a stability control method for the system with the IEPC is proposed. Furthermore, the validities of the proposed method are demonstrated by simulation and experiments. Finally, a restart performance of the system after momentary power interruption is discussed. Because the IEPC does not need phase-locked-loop to detect the phase angle of the input voltage, fast restart is expected. [ABSTRACT FROM AUTHOR]

This paper proposes a novel control strategy and a protection circuit and shows the advantage of utilizing a newly developed reverse blocking insulated gate bipolar transistor (RB-IGBT), to solve several practical problems of the matrix converter. The proposed control strategy is based on a virtual indirect control method with a virtual rectifier and a virtual inverter. Pulse-width modulated (PWM) pulses for the matrix converter are obtained by combining PWM pulses for the virtual rectifier and inverter. As a result, the control part of the input current and output voltage can be clearly separated. Thus, the conventional inverter control algorithms can be applied to the virtual inverter control. The advantage of this method is confirmed by experimental results with a 22-kW induction motor drive system. Good sinusoidal waveforms are obtained for the input and output currents, and the total harmonic distortion (THD) of the input and output current are 5.1% and 1.4%, respectively. The conduction loss of the RB-IGBT is decreased to about two-thirds of the conventional ac switch with series connection diode. Thus, the converter loss is about a half to the conventional PWM rectifier-inverter system with the same capacity. Furthermore, the protection problem is solved by a dynamic clamp method without an electrolytic capacitor. This protection circuit directly dissipates reactive load energy by dynamic clamp operation of an IGBT. [ABSTRACT FROM AUTHOR]

Rice is becoming a model plant in monocotyledons and a model cereal crop. For better understanding of the rice plant, it is essential to elucidate the developmental programs of the life cycle. To date, several attempts have been made in rice to categorize the developmental processes of some organs into substages. These studies are based exclusively on the morphological and anatomical viewpoints. Recent advancement in genetics and molecular biology has given us new aspects of developmental processes. In this review, we first describe the phasic development of the rice plant, and then describe in detail the developmental courses of major organs, leaf, root and spikelet, and specific organs/tissues. Also, for the facility of future studies, we propose a staging system for each organ. [ABSTRACT FROM AUTHOR]

Examines the effect of the SHOOTLESS2 (shl) and SHOOTLESS1 genes in both initiation and maintenance of the shoot apical meristem through regulating the number of indeterminate cells. Analysis of strains carrying weak alleles of shl; Formation of shoot meristem during embryogenesis by strains bearing the weak alleles; Establishment of proper organization of the shoot meristem.

Presents study which proposed two novel circuits which realized a unity input power factor single-phase to three-phase converter with a motor load. Methodology; Comparison between conventional circuit and proposed circuit; Results of the study; Conclusions.

In this paper, a DC–single-phase AC power converter with an LLC resonant converter is presented for a photovoltaic (PV) micro-inverter application. This application requires the leakage current suppression capability. Therefore, an isolated power converter is usually combined for DC/AC systems. The LLC resonant converter is the one of the isolated power converter topologies, and it has good performance for conversion efficiency with easy control. On the other hand, a double-line frequency power ripple has to be compensated for in order to improve the performance of the maximum power point tracking (MPPT). Therefore, a bulky electrolytic capacitor is usually necessary for the power converter. However, the electrolytic capacitor may limit the lifetime of the micro-inverter. This paper introduces the PV micro-inverter with a LLC resonant converter. In addition, the active power decoupling circuit is applied in order to compensate the double-line frequency power ripple by the small capacitor in order to eliminate the electrolytic capacitor. Finally, the transformer design is considered in order to reduce the transformer losses. As a result, the conversion efficiency of the LLC converter is improved by 1% when the litz wire has many strands. [ABSTRACT FROM AUTHOR]

This paper proposes a zero-voltage ride-through (ZVRT) method and an LCL filter design method to meet the fault ride-through (FRT) requirements for a single-phase grid-connected inverter with a minimized LCL filter. A high inverter output current overshoot occurs at a grid voltage sag and a grid voltage recovery, when a low-inductance LCL filter design is employed in order to minimize the filter volume. In order to suppress the current overshoot, a high-speed gate-block operation and the design method of the minimized LCL filter are proposed. In particular, the filter design considers the delay time in the gate-block operation in order to clarify the reduction limitation of the inductance in the LCL filter. As a result, the maximum inverter output current overshoot is suppressed to 146% by the high-speed gate-block operation and the designed LCL filter, where the impedances of an interconnected inductor and a filter inductor are 1.0% and 0.78% of the inverter normalized impedance, respectively. Therefore, it is possible to meet the FRT requirements with the proposed ZVRT method even with the minimized LCL filter. [ABSTRACT FROM AUTHOR]

Two proposed methods of reducing radiation noise caused by inductive power transfer (IPT) systems using spread spectrum techniques were experimentally demonstrated. In IPT systems for electric vehicles (EVs) or plug-in hybrid EVs (PHEVs), noise reduction techniques are necessary because the radiation noise from the IPT system for EVs or PHEVs must not exceed the limits defined by noise standards, such as the well-known regulation set by the International Special Committee on Radio Interference. In the proposed methods, the radiation noise from the transmission coils of the IPT system is spread in the frequency domain by randomly changing the output frequency of the inverter. The output frequency is selected according to the generation of pseudorandom numbers. In the first proposed method, spread spectrum with a uniform distribution (SSUD), the output frequency is selected from the frequency range of 80 to 90 kHz with an even distribution. In the second method, spread spectrum with a biased distribution (SSBD), the output current of the inverter is considered. The possibility of selecting an output frequency in the given range is biased in proportion to the combined impedance from the transmission coil and the resonance capacitors on the primary side. In experiments with a 3-kW prototype, when SSUD and SSBD were applied, the fundamental components of the radiation noise were suppressed by 4.45 and 8.27 dB · μA, respectively, in comparison with the noise obtained using the conventional system, which operates the inverter at a fixed frequency. [ABSTRACT FROM PUBLISHER]

This paper proposes a bidirectional isolated single-phase matrix converter with a center-tapped transformer, which is used in a battery storage system connecting to a single-phase ac load. The proposed converter consists of a full-bridge inverter, a high-frequency center-tapped transformer, and a matrix converter. The matrix converter is employed with a pulse density modulation in order to achieve zero-voltage switching (ZVS). On the other hand, a common-mode voltage of the center-tapped transformer and a small capacitor are utilized in order to absorb the power ripple of the single-phase ac load without additional switches. Furthermore, the switches in the full-bridge inverter partially achieve ZVS in order to reduce the switching loss. These characteristics result in a high-power-density converter. As the experimental results with a 1 kW-prototype, the proposed converter reduces the dc current ripple generated by a single-phase ac load by 80.2% in the discharging mode and 76.8% in the charge mode. Besides, the low ac-side voltage total harmonic distortion in the discharge are below 3.0% over the entire ac power range. [ABSTRACT FROM AUTHOR]

This paper proposes a current control method for discontinuous current mode (DCM) in order to achieve the same control performance as continuous current mode (CCM) in a boost converter. By utilizing the duty ratio at the previous calculation period to compensate for a DCM nonlinearity, the controller, which is designed for CCM, can also be used in DCM. In the frequency analysis, the cutoff frequency of the proposed DCM current control agrees exactly to the design value, which is 2 kHz, whereas the cutoff frequency of the conventional DCM current control results in high error of 47.5%. In the current step response experiment with a 360-W prototype and the switching frequency of 20 kHz, the experimental DCM current response almost agrees with the conventional CCM current response, which are 380-μs rise time for both CCM and DCM, 9% and 8% overshoot for CCM and DCM, respectively. Furthermore, the computation time of the proposed DCM current control is shorter 35% than the conventional DCM current control. [ABSTRACT FROM AUTHOR]

This paper proposes a new circuit configuration and a control scheme for a single-phase current-source inverter with a power decoupling circuit which is called as the active buffer. The proposed inverter achieves a low-dc-input voltage ripple and also provides a sinusoidal current that can achieve unity power factor, without large passive components in the dc bus such as smoothing inductors and electrolytic capacitors. These components are conventionally required in order to decouple the power pulsation caused by the single-phase power source. In this paper, the fundamental operations of the proposed inverter are demonstrated experimentally. From the experimental results, the input voltage ripple is 8.87%, and the output current total harmonic distortion is 4.24%. In addition, an output power factor of 99% and a maximum efficiency of 94.9% are obtained. Finally, it is confirmed that the maximum power densities of the conventional circuit and the proposed circuit are 2.75 kW/L at the switching frequency of 70 kHz and 4.86 kW/L at the switching frequency of 80 kHz, respectively. [ABSTRACT FROM AUTHOR]

Most aerial parts of the plant body are products of the continuous activity of the shoot apical meristem (SAM). Leaves are the major component of the aerial plant body, and their temporal and spatial distribution mainly determines shoot architecture. Here we report the identification of the rice gene PLASTOCHRON3 ( PLA3)/ GOLIATH ( GO) that regulates various developmental processes including the rate of leaf initiation (the plastochron). PLA3/ GO encodes a glutamate carboxypeptidase, which is thought to catabolize small acidic peptides and produce small signaling molecules. pla3 exhibits similar phenotypes to pla1 and pla2– a shortened plastochron, precocious leaf maturation and rachis branch-to-shoot conversion in the reproductive phase. However, in contrast to pla1 and pla2, pla3 showed pleiotropic phenotypes including enlarged embryo, seed vivipary, defects in SAM maintenance and aberrant leaf morphology. Consistent with these pleiotropic phenotypes, PLA3 is expressed in the whole plant body, and is involved in plant hormone homeostasis. Double mutant analysis revealed that PLA1, PLA2 and PLA3 are regulated independently but function redundantly. Our results suggest that PLA3 modulates various signaling pathways associated with a number of developmental processes. [ABSTRACT FROM AUTHOR]

The plastochron, the time interval between the formation of two successive leaves, is an important determinant of plant architecture. We genetically and phenotypically investigated many-noded dwarf (mnd) mutants in barley. The mnd mutants exhibited a shortened plastochron and a decreased leaf blade length, and resembled previously reported plastochron1 (pla1), pla2, and pla3 mutants in rice. In addition, the maturation of mnd leaves was accelerated, similar to pla mutants in rice. Several barley mnd alleles were derived from three genes—MND1, MND4, and MND8. Although MND4 coincided with a cytochrome P450 family gene that is a homolog of rice PLA1, we clarified that MND1 and MND8 encode an N-acetyltransferase-like protein and a MATE transporter-family protein, which are respectively orthologs of rice GW6a and maize BIGE1 and unrelated to PLA2 or PLA3. Expression analyses of the three MND genes revealed that MND1 and MND4 were expressed in limited regions of the shoot apical meristem and leaf primordia, but MND8 did not exhibit a specific expression pattern around the shoot apex. In addition, the expression levels of the three genes were interdependent among the various mutant backgrounds. Genetic analyses using the double mutants mnd4mnd8 and mnd1mnd8 indicated that MND1 and MND4 regulate the plastochron independently of MND8, suggesting that the plastochron in barley is controlled by multiple genetic pathways involving MND1, MND4, and MND8. Correlation analysis between leaf number and leaf blade length indicated that both traits exhibited a strong negative association among different genetic backgrounds but not in the same genetic background. We propose that MND genes function in the regulation of the plastochron and leaf growth and revealed conserved and diverse aspects of plastochron regulation via comparative analysis of barley and rice. Author summary: The number of leaves produced during a plant's lifetime is major determinant of plant architecture and affects the efficiency of photosynthesis and crop productivity. The leaf number is dependent on the temporal pattern of leaf initiation at the shoot apical meristem, which is termed the plastochron. The genetic factors involved in plastochron regulation have been identified in several plant species. However, whether the functions of plastochron-related genes and their genetic pathways are universal or diversified among different plant species is unclear. In this study, we investigated many-noded dwarf (mnd) mutants in barley, which exhibited a shortened plastochron and a decreased leaf blade length. The mutant alleles used in this study were derived from three genes, MND4, MND1, and MND8, which encode a cytochrome P450 family protein, an N-acetyltransferase-like protein, and a MATE transporter-family protein, respectively. Phenotypic and expression analyses revealed that these three MND genes affect the leaf production rate and leaf maturation program, but their expression levels were interdependent. In addition, the plastochron and leaf growth are closely related but independently regulated. We also analyzed the expression patterns and knockout mutants of rice MND orthologs to clarify whether their biological functions are conserved in rice and barley. This study provides insight into the genetic mechanisms of plastochron control in grass species. [ABSTRACT FROM AUTHOR]

Background: Rice leaves consist of three distinct regions along a proximal-distal axis, namely the leaf blade, sheath, and blade-sheath boundary region. Each region has a unique morphology and function, but the genetic programs underlying the development of each region are poorly understood. To fully elucidate rice leaf development and discover genes with unique functions in rice and grasses, it is crucial to explore genome-wide transcriptional profiles during the development of the three regions. Results: In this study, we performed microarray analysis to profile the spatial and temporal patterns of gene expression in the rice leaf using dissected parts of leaves sampled in broad developmental stages. The dynamics in each region revealed that the transcriptomes changed dramatically throughout the progress of tissue differentiation, and those of the leaf blade and sheath differed greatly at the mature stage. Cluster analysis of expression patterns among leaf parts revealed groups of genes that may be involved in specific biological processes related to rice leaf development. Moreover, we found novel genes potentially involved in rice leaf development using a combination of transcriptome data and in situ hybridization, and analyzed their spatial expression patterns at high resolution. We successfully identified multiple genes that exhibit localized expression in tissues characteristic of rice or grass leaves. Conclusions: Although the genetic mechanisms of leaf development have been elucidated in several eudicots, direct application of that information to rice and grasses is not appropriate due to the morphological and developmental differences between them. Our analysis provides not only insights into the development of rice leaves but also expression profiles that serve as a valuable resource for gene discovery. The genes and gene clusters identified in this study may facilitate future research on the unique developmental mechanisms of rice leaves. [ABSTRACT FROM AUTHOR]

Summary: Floods impede gas (O2 and CO2) exchange between plants and the environment. A mechanism to enhance plant gas exchange under water comprises gas films on hydrophobic leaves, but the genetic regulation of this mechanism is unknown. We used a rice mutant (dripping wet leaf 7, drp7) which does not retain gas films on leaves, and its wild‐type (Kinmaze), in gene discovery for this trait. Gene complementation was tested in transgenic lines. Functional properties of leaves as related to gas film retention and underwater photosynthesis were evaluated. Leaf Gas Film 1 (LGF1) was identified as the gene determining leaf gas films. LGF1 regulates C30 primary alcohol synthesis, which is necessary for abundant epicuticular wax platelets, leaf hydrophobicity and gas films on submerged leaves. This trait enhanced underwater photosynthesis 8.2‐fold and contributes to submergence tolerance. Gene function was verified by a complementation test of LGF1 expressed in the drp7 mutant background, which restored C30 primary alcohol synthesis, wax platelet abundance, leaf hydrophobicity, gas film retention, and underwater photosynthesis. The discovery of LGF1 provides an opportunity to better understand variation amongst rice genotypes for gas film retention ability and to target various alleles in breeding for improved submergence tolerance for yield stability in flood‐prone areas. [ABSTRACT FROM AUTHOR]

In the present, a power decoupling method without additional component is proposed for a dc to single-phase ac converter, which consists of a flying capacitor dc/dc converter (FCC) and the voltage source inverter (VSI). In particular, a small flying capacitor in the FCC is used for both a boost operation and a double-line-frequency power ripple reduction. Thus, the dc-link capacitor value can be minimized in order to avoid the use of a large electrolytic capacitor. In addition, component design, of, e.g., the boost inductor and the flying capacitor, is clarified when the proposed control is applied. Experiments were carried out using a 1.5-kW prototype in order to verify the validity of the proposed control. The experimental results revealed that the use of the proposed control reduced the dc-link voltage ripple by 74.5%, and the total harmonic distortion (THD) of the inverter output current was less than 5%. Moreover, a maximum system efficiency of 95.4% was achieved at a load of 1.1 kW. Finally, the high power density design is evaluated by the Pareto front optimization. The power densities of three power decoupling topologies, such as a boost topology, a buck topology, and the proposed topology are compared. As a result, the proposed topology achieves the highest power density (5.3 kW/dm3) among the topologies considered herein. [ABSTRACT FROM AUTHOR]

In several eudicot species, one copy of each member of the WUSCHEL-RELATED HOMEOBOX (WOX) gene family, WOX1 and WOX3, is redundantly or differentially involved in lateral leaf outgrowth, whereas only the WOX3 gene regulating the lateral domain of leaf development has been reported in grass. In this study, we show that a WOX3 gene, LEAF LATERAL SYMMETRY1 (LSY1), regulates lateral leaf development in a different manner ftom that of other duplicated paralogs of WOX3, NARROW LEAF2 (NAL2)/NAL3, in rice. A loss-of-function mutant of LSY1 exhibited an asymmetrical defect from early leaf development, which is different from a symmetric defect in a double loss-of-function mutant of NAL2/3, whereas the expression of both genes was observed in a similar domain in the margins of leaf primordia. Unlike NAL2/3, overexpression of LSY1 produced malformed leaves whose margins were curled adaxially. Expression domains and the level of adaxial/abaxial marker genes were affected in the LSY1-overexpressing plants, indicating that LSY1 is involved in regulation of adaxial-abaxial patterning at the margins of the leaf primordia. Additive phenotypes in some leaf traits of lsy1 nal2/3 triple mutants and the unchanged level of NAL2/3 expression in the lsy1 background suggested that LSY1 regulates lateral leaf development independently of NAL2/3. Our results indicated that all of the rice WOX3 genes are involved in leaf lateral outgrowth, but the functions of LSY1 and NAL2/3 have diverged. We propose that the function of WOX3 and the regulatory mode of leaf development in rice are comparable with those of WOX1/WOX3 in eudicot species. [ABSTRACT FROM AUTHOR]

Auxin is a fundamental plant hormone and its localization within organs plays pivotal roles in plant growth and development. Analysis of many Arabidopsis mutants that were defective in auxin biosynthesis revealed that the indole-3-pyruvic acid ( IPA) pathway, catalyzed by the TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS ( TAA) and YUCCA ( YUC) families, is the major biosynthetic pathway of indole-3-acetic acid ( IAA). In contrast, little information is known about the molecular mechanisms of auxin biosynthesis in rice. In this study, we identified a auxin-related rice mutant, fish bone ( fib). FIB encodes an orthologue of TAA genes and loss of FIB function resulted in pleiotropic abnormal phenotypes, such as small leaves with large lamina joint angles, abnormal vascular development, small panicles, abnormal organ identity and defects in root development, together with a reduction in internal IAA levels. Moreover, we found that auxin sensitivity and polar transport activity were altered in the fib mutant. From these results, we suggest that FIB plays a pivotal role in IAA biosynthesis in rice and that auxin biosynthesis, transport and sensitivity are closely interrelated. [ABSTRACT FROM AUTHOR]

Maintenance of organ separation is one of the essential phenomena for normal plant development. We have identified and analyzed ONION3 (ONI3), which is required for avoiding organ fusions in rice. Loss-of-function mutations of ONI3, which were identified as mutants with ectopic expression of KNOX genes in leaves and morphologically resembling KNOX overexpressors, showed abnormal organ fusions in developing shoots. The mutant seedlings showed fusions between neighboring organs and also within an organ; they stopped growing soon after germination and subsequently died. ONI3 was shown to encode an enzyme that is most similar to Arabidopsis HOTHEAD and is involved in biosynthesis of long-chain fatty acids. Expression analyses showed that ONI3 was specifically expressed in the outermost cell layer in the shoot apex throughout life cycle, and the oni3 mutants had an aberrant outermost cell layer. Our results together with previous studies suggest that long-chain fatty acids are required for avoiding organ fusions and promoting normal shoot development in rice. [ABSTRACT FROM PUBLISHER]

Summary The temporal and spatial control of meristem identity is a key element in plant development. To better understand the molecular mechanisms that regulate inflorescence and flower architecture, we characterized the rice aberrant panicle organization 2 ( apo2) mutant which exhibits small panicles with reduced number of primary branches due to the precocious formation of spikelet meristems. The apo2 mutants also display a shortened plastochron in the vegetative phase, late flowering, aberrant floral organ identities and loss of floral meristem determinacy. Map-based cloning revealed that APO2 is identical to previously reported RFL gene, the rice ortholog of the Arabidopsis LEAFY ( LFY) gene. Further analysis indicated that APO2/RFL and APO1, the rice ortholog of Arabidopsis UNUSUAL FLORAL ORGANS, act cooperatively to control inflorescence and flower development. The present study revealed functional differences between APO2/RFL and LFY. In particular, APO2/RFL and LFY act oppositely on inflorescence development. Therefore, the genetic mechanisms for controlling inflorescence architecture have evolutionarily diverged between rice (monocots) and Arabidopsis (eudicots). [ABSTRACT FROM AUTHOR]

Because plant reproductive development occurs only in adult plants, the juvenile-to-adult phase change is an indispensable part of the plant life cycle. We identified two allelic mutants, peter pan syndrome-1 (pps-1) and pps-2 , that prolong the juvenile phase in rice (Oryza sativa) and showed that rice PPS is an ortholog of Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENIC1. The pps-1 mutant exhibits delayed expression of miR156 and miR172 and the suppression of GA biosynthetic genes, reducing the GA3 content in this mutant. In spite of its prolonged juvenile phase, the pps-1 mutant flowers early, and this is associated with derepression of RAP1B expression in pps-1 plants independently of the Hd1 - Hd3a/RFT1 photoperiodic pathway. PPS is strongly expressed in the fourth and fifth leaves, suggesting that it regulates the onset of the adult phase downstream of MORI1 and upstream of miR156 and miR172. Its ability to regulate the vegetative phase change and the time of flowering suggests that rice PPS acquired novel functions during the evolution of rice/monocots. [ABSTRACT FROM AUTHOR]

Abstract: The adaxial–abaxial axis in leaf primordia is thought to be established first and is necessary for the expansion of the leaf lamina along the mediolateral axis. To understand axis information in leaf development, we isolated the adaxialized leaf1 (adl1) mutant in rice, which forms abaxially rolled leaves. adl1 leaves are covered with bulliform-like cells, which are normally distributed only on the adaxial surface. An adl1 double mutant with the adaxially snowy leaf mutant, which has albino cells that specifically appear in the abaxial mesophyll tissue, indicated that adl1 leaves show adaxialization in both epidermal and mesophyll tissues. The expression of HD-ZIPIII genes in adl1 mutant increased in mature leaves, but not in the young primordia or the SAM. This indicated that ADL1 may not be directly involved in determining initial leaf polarity, but rather is associated with the maintenance of axis information. ADL1 encodes a plant-specific calpain-like cysteine proteinase orthologous to maize DEFECTIVE KERNEL1. Furthermore, we identified intermediate and strong alleles of the adl1 mutant that generate shootless embryos and globular-arrested embryos with aleurone layer loss, respectively. We propose that ADL1 plays an important role in pattern formation of the leaf and embryo by promoting proper epidermal development. [Copyright &y& Elsevier]

Numerous genes are involved in the regulation of plant development, including those that regulate floral homeotic genes, We identified two recessive allelic rice mutants, open beak-1 ( opb-1) and opb-2, which exhibited pleiotropic defects in leaf morphogenesis, inflorescence architecture, and floral organ identity. Abnormal cell proliferation was observed in the leaves and spikelets, and ectopic or overexpression of several class 1 knox genes was detected; thus, the abnormal cell proliferation in opb mutants is probably caused by ectopic class 1 knox gene expression. The opb mutants also had defects in floral organ identity, resulting in the development of mosaic organs, including gluminous lodicules, staminoid lodicules, and pistiloid stamens. These results, together with the reduced expression of a class B gene, indicate that OPB positively regulates the expression of class B genes. Map-based cloning revealed that OPB encodes a transcription factor that is orthologous to the Arabidopsis JAGGED gene and is expressed in leaf primordia, inflorescence meristem, rachis branch meristems, floral meristem, and floral organ primordia. Taken together, our data suggest that the OPB gene affects cellular proliferation and floral organ identity through the regulation of class 1 knox genes and floral homeotic genes. [ABSTRACT FROM AUTHOR]

Abstract: We isolated flattened shoot meristem (fsm) mutants in rice that showed defective seedling growth and died in the vegetative phase. Since most fsm plants had flat and small shoot apical meristems (SAMs), we suggest that FSM is required for proper SAM maintenance. FSM encodes a putative ortholog of Arabidopsis FASCIATA1 (FAS1) that corresponds to the p150 subunit of chromatin assembly factor-1 (CAF-1). FSM is expressed patchily in tissues with actively dividing cells, suggesting a tight association of FSM with specific cell-cycle phases. Double-target in situ hybridization counterstained with cell-cycle marker genes revealed that FSM is expressed mainly in the G1 phase. In fsm, expressions of the two marker genes representing S- and G2- to M-phases were enhanced in SAM, despite a reduced number of cells in SAM, suggesting that S- and G2-phases are prolonged in fsm. In addition, developmental events in fsm leaves took place at the proper time, indicating that the temporal regulation of development occurs independently of the cell-cycle period. In contrast to the fasciated phenotype of Arabidopsis fas1, fsm showed size reduction of SAM. The opposite phenotypes between fsm and fas1 indicate that the SAM maintenance is regulated differently between rice and Arabidopsis. [Copyright &y& Elsevier]

During postembryonic development of higher plants, the shoot apical meristem produces lateral organs in a regular spacing (phyllotaxy) and a regular timing (plastochron). Molecular analysis of mutants associated with phyllotaxy and plastochron would greatly increase understanding of the developmental mechanism of plant architecture because phyllotaxy and plastochron are fundamental regulators of plant architecture. pla 1 of rice is not only a plastochron mutant showing rapid leaf initiation without affecting phyllotaxy, but also a heterochronic mutant showing ectopic shoot formation in the reproductive phase. Thus, pla1 provides a tool for analyzing the molecular basis of temporal regulation in leaf development. In this work, we isolated the PLA1 gene by map-based cloning. The identified PLA1 gene encodes a cytochrome P450, CYP78A11, which potentially catalyzes substances controlling plant development. PLA1 is expressed in developing leaf primordia, bracts of the panicle, and elongating internodes, but not in the shoot apical meristem. The expression pattern and mutant phenotype suggest that the PLA1 gene acting in developing leaf primordia affects the timing of successive leaf initiation and the termination of vegetative growth. [ABSTRACT FROM AUTHOR]