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Zinc finger protein 804A (ZNF804A) has been identified by genome-wide association studies as a robust risk gene in schizophrenia, but how ZNF804A contributes to schizophrenia and its upstream regulation remains unknown. Previous studies have indicated that microRNAs (miRs) are key factors that regulate the expression levels of their target genes. The present study revealed significantly increased expression of miR-148b-3p in the peripheral blood of patients with first-onset schizophrenia compared with healthy controls, and bioinformatics analysis predicted that the ZNF804A gene is a target of miR-148b-3p. Therefore, the present study investigated the possible upstream regulation of ZNF804A by miR-148b-3p in the human neuroblastoma SH-SY5Y cell line, and assessed the implications for schizophrenia. The results revealed significantly reversed expression levels of miR-148b-3p (P=0.0051) and ZNF804A (P=0.0218) in the peripheral blood of patients with first-onset schizophrenia compared with healthy individuals. Furthermore, it was demonstrated that miR-148b-3p directly targeted ZNF804A via binding to conserved target sites in the 3′-untranslated region of ZNF804A mRNA, where it inhibited the endogenous expression of ZNF804A at both the mRNA (P=0.048) and protein levels (P=0.013) in SH-SY5Y cells. Furthermore, miR-148b-3p was revealed to regulate the expression levels of catechol-O-methyltransferase (COMT) and serine protease 16 (PRSS16) by targeting ZNF804A in SH-SY5Y cells. Collectively, the present results indicated that there was a direct upstream regulation of the schizophrenia risk gene ZNF804A by miR-148b-3p, which contributed to the regulation of the downstream genes COMT and PRSS16. Thus, the miR-148b-3p/ZNF804A/COMT/PRSS16 pathway may play an important role in the pathophysiology of schizophrenia, and may serve as a potential target in drug discovery and gene therapy for this disorder.

NMDA receptors play an important physiological role in regulating synaptic plasticity, learning and memory. GluN2A subunits are the most abundant functional subunits of NMDA receptors expressed in mature brain, and their dysfunction is related to various neurological diseases. According to subunit composition, GluN2A-containing NMDA receptors can be divided into two types: diheteromeric and triheteromeric receptors. In this review, the expression, functional and pharmacological properties of different kinds of GluN2A-containing NMDA receptors as well as selective GluN2A regulators were described to further understand this type of NMDA receptors.

With the continuous development and expansion of the city, the industrial center of the city has gradually shifted to the emerging industrial area or the outskirts. The industrial building remains that were originally located in the city center have been left behind. The development of the site often adopts the development method of "buy the casket without the jewels ", while the development method of "buy the casket with the jewels "is few, and which development method is more valuable.Taking Laolongkou distillery as an example, this paper makes a comparative analysis in order to provide reference for the site development of industrial building remains.

This paper discusses the establishment and construction of characteristic towns at all levels in Liaoning Province, including 13 national characteristic towns, 20 provincial characteristic villages and towns and 11 industrial characteristic towns, and then analyzed the problems and obstacles encountered in the construction of these towns, puts forward some suggestions and strategies to solve these problems, in order to provide some reference for the construction of characteristic towns in Liaoning Province.

As an old industrial base in the country, Liaoning Province has a solid industrial foundation. The development of industrial tourism in Liaoning Province can not only accelerate the promotion of new industrialization roads, but also contribute to the healthy and sustainable development of the tourism industry in Liaoning Province. This paper analyzes the obstacles and problems in the development of industrial tourism in Liaoning Province at the current stage, and proposes a series of countermeasures to promote the development of industrial tourism in Liaoning Province, which provides ideas for the development and revitalization of industrial tourism.

Background Although pneumonia is a known cause of morbidity and mortality in Sarawak, Malaysia, the etiology and epidemiology of pneumonia are not well described in this equatorial region. Routine clinical diagnostics for pneumonia etiology at government hospitals in Sarawak had historically involved only bacterial diagnostics. Viral diagnostics were only obtained through outside consultations. Methods From June 15, 2017 to May 14, 2018, we collected nasopharyngeal swabs from 600 patients of all ages older than 1 month hospitalized with pneumonia at Sibu and Kapit Hospitals. Specimens were examined at our collaborating institutions with a panel of molecular assays for viral pathogens including influenza A (IAV), IBV, ICV, and IDV, human adenovirus (AdV), human enterovirus (EV), human coronavirus (CoV), respiratory syncytial virus subtype A (RSV-A) or RSV-B, and parainfluenza virus (PIV) types 1–4. Results Of 599 samples examined, 288 (48%) had molecular evidence of 1 or more respiratory viruses. Overall, the most prevalent virus detected was RSV-A (14.2%) followed by AdV (10.4%) and IAV (10.4%), then RSV-B (6.2%), EV (4.2%), IBV (2.2%), PIV-3 (1.7%), CoV (1.0%), PIV-1 (1.0%), PIV-4 (0.7%), and PIV-2 (0.2%). No specimens were confirmed positive for ICV or IDV. Conclusions The high prevalence of viruses detected in this study suggest that respiratory viruses may be responsible for considerable morbidity in equatorial regions such as Sarawak. Access to viral diagnostics are very necessary for medical staff to determine appropriate pneumonia treatments.

Oxadiazole as a class of five-membered heterocycles has been reported to be biologically versatile compounds due to their metabolic profile. Amongst these heterocycles, 1,3,4oxadiazole has become an important construction motif for the development of new drugs, furamizole as antibiotics, tiodazosin as antihypertensive agent and raltegravir as antiretroviral agent have been on market. In addition, 2,5-disubstituted-1,3,4-oxadiazole derivatives have also been used as electron conducting and hole blocking (ECHB) materials for electron deficient and good electron transport properties of oxadiazole rings. The most common synthetic approach to 1,3,4-oxadiazoles involves cyclodehydration of 1,2-diacylhydrazines 3 (Scheme 1). Typically, this reaction is carried out by using SOCl2, 5 POCl3, 6 as well as others. Although these methods are very useful for the preparation of large quantities of materials due to the ready availability of diacylhydrazines, hydrazides, and hydrazones as starting materials, the reaction conditions tend to be harsh and long reaction times are generally needed. An alternative route to 1,3,4-oxadiazoles 2 by oxidative cyclization from the corresponding aldehyde N-acylhydrazones 1 proceeds (a) with transition metal oxidants such as Pb, Mn, Fe, Ce or Cu et al.; (b) with chloramine T, Trichloroisocyanuric Acid, iodobenzene diacetate (IBD), Dess–Martin reagent (DMP) or other oxidants; (c) electrochemical methods (Scheme 1). To the best of our knowledge, preparation of 1,3,4-oxadiazoles utilizing iodosobenzene as the oxidizing agent has not been reported yet. In our continuous research program of the oxidation of hypervalent iodine, we were interested in the development of robust and general method to access functionalized 2,5-disubstituted-1,3,4-oxadiazoles. It was found that aromatic aldehyde N-acylhydrazones 1 reacted with (PhIO)n/BF3·Et2O at room temperature to efficiently afford 2,5-disubstituted-1,3,4-oxadiazoles 2 in mild to good yields (Scheme 2). The polymeric character of iodosobenzene makes it less suitable as oxidant than other hypervalent reagents, mainly because of its insolubility in ordinary solvents. Its utility is, however, greatly enhanced when it is used with catalysts, notably boron trifluoride. The electrophilic character of iodine in iodosobenzene is greatly increased by the addition of boron trifluoride, probably because of the in-situ formation of the monomeric dipole PhI+-OBF3 −. N'-Benzylidenefuran-2-carbohydrazide (1a) was chosen as the substrate in initial studies (Table 1). When the substrate

Background The large livestock operations and dense human population of Southeast Asia are considered a hot-spot for emerging viruses. Objectives To determine if the pathogens adenovirus (ADV), coronavirus (CoV), encephalomyocarditis virus (EMCV), enterovirus (EV), influenza A-D (IAV, IBV, ICV, and IDV), porcine circovirus 2 (PCV2), and porcine rotaviruses A and C (RVA and RVC), are aerosolized at the animal-interface, and if humans working in these environments are carrying these viruses in their nasal airways. Study This cross-sectional study took place in Sarawak, Malaysia among 11 pig farms, 2 abattoirs, and 3 animal markets in June and July of 2017. Pig feces, pig oral secretions, bioaerosols, and worker nasal wash samples were collected and analyzed via rPCR and rRT-PCR for respiratory and diarrheal viruses. Results In all, 55 pig fecal, 49 pig oral or water, 45 bioaerosol, and 78 worker nasal wash samples were collected across 16 sites. PCV2 was detected in 21 pig fecal, 43 pig oral or water, 3 bioaerosol, and 4 worker nasal wash samples. In addition, one or more bioaerosol or pig samples were positive for EV, IAV, and RVC, and one or more worker samples were positive for ADV, CoV, IBV, and IDV. Conclusions This study demonstrates that nucleic acids from a number of targeted viruses were present in pig oral secretions and pig fecal samples, and that several viruses were detected in bioaerosol samples or in the nasal passages of humans with occupational exposure to pigs. These results demonstrate the need for future research in strengthening viral surveillance at the human-animal interface, specifically through expanded bioaerosol sampling efforts and a seroepidemiological study of individuals with exposure to pigs in this region for PCV2 infection.

The species of Cr(III) and Cr(VI) in water samples were determined by flow injection on-line preconcentration and separation on two-microcolumn system-derivative flame atomic absorption spectrometry during a collaborative analysis for certification. The Cr(III) and Cr(VI) in water samples were retained on two microcolumns with ion exchange resin and were eluted directly to nebulizer by 15% HNO3 and 8% NH4NO3, respectively. The characteristic concentration (at the sensitivity grade of 2 mV min−1 for 1 min of preconcentration time) for Cr(III) and Cr(VI) were 0.130 and 0.0985 μg l−1, in the order which were 332- and 431-fold better than those of FAAS, and 45- and 47-fold better than those of FI-FAAS, respectively. The relative standard deviations were 3.27% and 3.66% with corresponding detection limits (3σ) of 0.244 and 0.235 μg l−1, respectively. The linear ranges of determinations for Cr(III) and Cr(VI) were 0∼100 μgm l−1 with correlation coefficients of 0.9984 to 0.9996. The satisfactory recovery of 94.4%∼106% for Cr(III) and Cr(VI) could be obtained from water samples.

A rapid and sensitive method has been proposed for the sequential determination of chromium(III) and total chromium in water samples by flame atomic absorption spectrometry combined with a flow injection on-line preconcentration on a double-microcolumn. The chromium(III) and total chromium in samples were retained on a double-microcolumn with a cation exchange resin, respectively, and eluted directly into a nebulizer by 3 mol L(-1) HNO3. The characteristic concentration (gives a derivative absorbance of 0.0044) and the detection limit (3sigma) for chromium were 0.512 microg L(-1) and 0.647 microg L(-1) for a preconcentration time of 1 min, respectively. This is an improvement of 20 and 14-times than those of conventional FI-FAAS. The proposed method allows the determination of chromium in the range of 0-90 microg L(-1) with a relative standard deviation of 3.63% at the 10 microg L(-1) level. The method has been applied for the analysis of chromium in reference water of National Research Center for Certified Reference Materials (GBW08607) and other water samples with satisfactory results.

A new method is developed for the direct determination of trace and ultra-trace level of arsenic and antimony in waters by hydride generation derivative atomic absorption spectrometry (DHGAAS). The signal model and fundamentals of DHGAAS are described. The effects of atomization temperature, argon flow rate, acidity and concentration of KBH(4)and KI were investigated and analytical conditions were optimized. The sensitivities for arsenic and antimony were increased 36.4 and 27.6 times better than those of conventional hydride generation atomic absorption spectrometry (HGAAS). For a 2 mV min(-1) sensitivity range setting, the characteristic concentration was 0.003 microg L(-1) for arsenic and 0.004 microg L(-1)for antimony, and the detection limits (3sigma) were 0.015 micro g L(-1) for arsenic and 0.020 microg L(-1) for antimony. The proposed method was applied to the determination of arsenic and antimony in several water samples with satisfactory results.

Most works focused on the Trichel pulses at an atmosphere pressure in the past. In this paper, the characteristics of Trichel pulses at low pressure (several Torr) in air used by a needle-to-plane discharge structure are investigated. The current-voltage (I-V) characteristics, the images of discharge and the time evolution of the current and voltage of Trichel pulses are measured under different conditions. Results show that Trichel pulses exist in the negative differential resistance of I-V curves, and disappear when the discharge transits to the abnormal glow discharge mode with an abrupt contraction of discharge area around the needle tip. The pulses are consisted of a very short rising time (about 10 μs) and a short durations time (tens of μs) separated by longer inter-pulse periods (more than 100 μs). The frequency of pulses can be tuned continuously from a few kilohertz to more than ten kilohertz by adjusting the averaged current and the pressure. It increases with the increase of averaged current at the same pressure, and rises as the pressure increasing at the same averaged current. These results will be helpful to understand the mechanism of Trichel pulses.

The title pyrimidine derivative, C(18)H(21)N(3)O(6), was obtained by the reaction of methyl 2-[2-(benzyl-oxycarbon-yl)amino-propan-2-yl]-5-hy-droxy-6-oxo-1,6-dihydro-pyrimidine-4-carboxyl-ate with dimethyl sulfate in dimethyl sulfoxide. The mol-ecule has a V-shaped structure, the phenyl and the pyrimidine rings making a dihedral angle of 43.1 (1)°. The methyl group substituting the pyrimidine ring deviates slightly from the ring mean-plane [C-N-C-C torsion angle = 5.49 (15)°], and the methyl ester substituent has a conformation suitable for the formation of an intra-molecular O-H⋯O hydrogen bond with the hydroxyl functionality. In the crystal, molecules are linked into chains along the b axis by N-H⋯O hydrogen bonds.