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5. Corrigendum: Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)

Author

Carlsen, Frida Meijer, primary, Johansen, Ida Elisabeth, additional, Yang, Zhang, additional, Liu, Ying, additional, Westberg, Ida Nøhr, additional, Kieu, Nam Phuong, additional, Jørgensen, Bodil, additional, Lenman, Marit, additional, Andreasson, Erik, additional, Nielsen, Kåre Lehmann, additional, Blennow, Andreas, additional, and Petersen, Bent Larsen, additional

Published
2022
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6. Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)

Author

Carlsen, Frida Meijer, primary, Johansen, Ida Elisabeth, additional, Yang, Zhang, additional, Liu, Ying, additional, Westberg, Ida Nøhr, additional, Kieu, Nam Phuong, additional, Jørgensen, Bodil, additional, Lenman, Marit, additional, Andreasson, Erik, additional, Nielsen, Kåre Lehmann, additional, Blennow, Andreas, additional, and Petersen, Bent Larsen, additional

Published
2022
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11. The IRE1/bZIP60 Pathway and Bax Inhibitor 1 Suppress Systemic Accumulation of Potyviruses and Potexviruses in Arabidopsis and Nicotiana benthamiana Plants

Author

Gaguancela, Omar Arias, primary, Zúñiga, Lizbeth Peña, additional, Arias, Alexis Vela, additional, Halterman, Dennis, additional, Flores, Francisco Javier, additional, Johansen, Ida Elisabeth, additional, Wang, Aiming, additional, Yamaji, Yasuyuki, additional, and Verchot, Jeanmarie, additional

Published
2016
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12. The Balance between the MIR164A and CUC2 Genes Controls Leaf Margin Serration in Arabidopsis

Author

Hasson, Alice, Pulido, Amada, Vialette-Guiraud, Aurélie, Hay, Angela, Johansen, Ida Elisabeth, Tsiantis, Miltos, Raman, Smita, Greb, Thomas, Theres, Klaus, Nikovics, Krisztina, Blein, Thomas, Peaucelle, Alexis, Ishida, Tetsuya, Morin, Halima, Aida, Mitsuhiro, Laufs, Patrick, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Plant Biochemistry, Cell and Molecular Biology, Reproduction et développement des plantes (RDP), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Max Planck Institute for Plant Breeding Research (MPIPZ), Department of Plant and Environmental Sciences, department of Plant, Department of Plant Sciences, University of Oxford [Oxford], Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OeAW), Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Grad Sch Biol Sci, Nara Institute of Science and Technology, École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), and Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Mutant, Arabidopsis, Gene Expression, Plant Science, Flowers, Genes, Plant, 01 natural sciences, Models, Biological, Conserved sequence, 03 medical and health sciences, Gene expression, Botany, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Primordium, Research Articles, Alleles, Conserved Sequence, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, biology, Arabidopsis Proteins, fungi, Genetic Complementation Test, Wild type, food and beverages, Cell Biology, Meristem, biology.organism_classification, Plants, Genetically Modified, Cell biology, Plant Leaves, Serration, MicroRNAs, Mutation, Nucleic Acid Conformation, 010606 plant biology & botany
Abstract

CUP-SHAPED COTYLEDON1 (CUC1), CUC2, and CUC3 define the boundary domain around organs in the Arabidopsis thaliana meristem. CUC1 and CUC2 transcripts are targeted by a microRNA (miRNA), miR164, encoded by MIR164A, B, and C. We show that each MIR164 is transcribed to generate a large population of primary miRNAs of variable size with a locally conserved secondary structure around the pre-miRNA. We identified mutations in the MIR164A gene that deepen serration of the leaf margin. By contrast, leaves of plants overexpressing miR164 have smooth margins. Enhanced leaf serration was observed following the expression of an miR164-resistant CUC2 but not of an miR164-resistant CUC1. Furthermore, CUC2 inactivation abolished serration in mir164a mutants and the wild type, whereas CUC1 inactivation did not. Thus, CUC2 specifically controls leaf margin development. CUC2 and MIR164A are transcribed in overlapping domains at the margins of young leaf primordia, with transcription gradually restricted to the sinus, where the leaf margins become serrated. We suggest that leaf margin development is controlled by a two-step process in Arabidopsis. The pattern of serration is determined first, independently of CUC2 and miR164. The balance between coexpressed CUC2 and MIR164A then determines the extent of serration.

Published
2006

13. Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana.

Author

Weiqiang Qian, Chunmei Yu, Huanju Qin, Xin Liu, Aimin Zhang, Johansen, Ida Elisabeth, and Daowen Wang

Subjects
FUNCTIONAL analysis, ARABIDOPSIS, AMINO acid sequence, AMINO acid analysis, NICOTIANA, BIOSYNTHESIS
Abstract

Phosphomannomutase (PMM) catalyzes the interconversion of mannose-6-phosphate and mannose-1-phosphate. However, systematic molecular and functional investigations on PMM from higher plants have hitherto not been reported. In this work, PMM cDNAs were isolated from Arabidopsis, Nicotiana benthamiana, soybean, tomato, rice and wheat. Amino acid sequence comparisons indicated that plant PMM proteins exhibited significant identity to their fungal and mammalian orthologs. In line with the similarity in primary structure, plant PMM complemented the sec53-6 temperature sensitive mutant of Saccharomyces cerevisiae. Histidine-tagged Arabidopsis PMM (AtPMM) purified from Escherichia coli converted mannose-1-phosphate into mannose-6-phosphate and glucose-1-phosphate into glucose-6-phosphate, with the former reaction being more efficient than the latter one. In Arabidopsis and N. benthamiana, PMM was constitutively expressed in both vegetative and reproductive organs. Reducing the PMM expression level through virus-induced gene silencing caused a substantial decrease in ascorbic acid (AsA) content in N. benthamiana leaves. Conversely, raising the PMM expression level in N. benthamiana using viral-vector-mediated ectopic expression led to a 20–50% increase in AsA content. Consistent with this finding, transgenic expression of an AtPMM–GFP fusion protein in Arabidopsis also increased AsA content by 25–33%. Collectively, this study improves our understanding on the molecular and functional properties of plant PMM and provides genetic evidence on the involvement of PMM in the biosynthesis of AsA in Arabidopsis and N. benthamiana plants. [ABSTRACT FROM AUTHOR]

Published
2007
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