73 results on '"Katona M."'
Search Results
2. Birth Weight Discordance in Spontaneous Versus Induced Twins: Impact on Perinatal Outcome
- Author
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Zádori, J., Kozinszky, Z., Orvos, H., Katona, M., Kaáli, S. G., and Pál, A.
- Published
- 2004
- Full Text
- View/download PDF
3. Dilemma of Increased Obstetric Risk in Pregnancies Following IVF-ET
- Author
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Zádori, J., Kozinszky, Z., Orvos, H., Katona, M., Pál, A., and Kovács, L.
- Published
- 2003
- Full Text
- View/download PDF
4. Interaction of nilotinib, dasatinib and bosutinib with ABCB1 and ABCG2: implications for altered anti-cancer effects and pharmacological properties
- Author
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Hegedűs, C, Özvegy-Laczka, C, Apáti, Á, Magócsi, M, Német, K, Őrfi, L, Kéri, G, Katona, M, Takáts, Z, Váradi, A, Szakács, G, and Sarkadi, B
- Published
- 2009
- Full Text
- View/download PDF
5. Birth Weight Discordance in Spontaneous Versus Induced Twins: Impact on Perinatal Outcome
- Author
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Z´dori, J., Kozinszky, Z., Orvos, H., Katona, M., Ka´li, S. G., and P´l, A.
- Published
- 2004
6. The Incidence of Major Birth Defects Following In Vitro Fertilization
- Author
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Zàdori, J, Kozinszky, Z, Orvos, H, Katona, M, Kaàli, S G., and Pàl, A
- Published
- 2003
7. Short Communication: The Incidence of Major Birth Defects Following In Vitro Fertilization
- Author
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Zádori, J., Kozinszky, Z., Orvos, H., Katona, M., Kaáli, S. G., and Pál, A.
- Published
- 2003
- Full Text
- View/download PDF
8. Report on the Results of Inoculation in Measles
- Author
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Von Katona, M.
- Published
- 1844
9. Inoculation of the Measles
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Von Katona, M.
- Published
- 1842
10. Functional presence of Na+-K+-2Cl- cotransporter in rabbit lacrimal gland ductal epithelial cells
- Author
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TÓTH-MOLNÁR, E, primary, KATONA, M, additional, VIZVARI, E, additional, FACSKO, A, additional, VENGLOVECZ, V, additional, RAKONCZAY, Z, additional, and HEGYI, P, additional
- Published
- 2014
- Full Text
- View/download PDF
11. Experimental evidence of fluid secretion of rabbit lacrimal gland ductal epithelia
- Author
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TOTH-MOLNAR, E, primary, KATONA, M, additional, FACSKO, A, additional, VENGLOVECZ, V, additional, NEMETH, L, additional, and HEGYI, P, additional
- Published
- 2013
- Full Text
- View/download PDF
12. P47.10: Possible etiological role of recurrent cytomegalovirus infection in case of non-immune hydrops fetalis
- Author
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Sikovanyecz, J., primary, Orvos, H., additional, Keresztúri, A., additional, Katona, M., additional, Deák, J., additional, Szabó, J., additional, and Pál, A., additional
- Published
- 2007
- Full Text
- View/download PDF
13. Cessation of fetal supraventricular tachycardia after compression of the umbilical cord
- Author
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Páal, A., primary and Katona, M., additional
- Published
- 1997
- Full Text
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14. Modified Blalock-Taussig Shunt Using Allograft Saphenous Vein: Six Years' Experience
- Author
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Bogats, G., Kertesz, E., Katona, M., Toszegi, A., and Kovacs, G. S.
- Published
- 1996
- Full Text
- View/download PDF
15. A Viscoelastic-Plastic Constitutive Model with a Finite Element Solution Methodology
- Author
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CIVIL ENGINEERING LAB (NAVY) PORT HUENEME CA, Katona, M., CIVIL ENGINEERING LAB (NAVY) PORT HUENEME CA, and Katona, M.
- Abstract
A textbook style development of viscoplasticity is presented. For completeness, the report also includes a detailed review of plasticity and viscoelasticity. The combination of plasticity and viscoelasticity is the basis for the so-called viscoelastic-plastic model developed herein. Other combo- viscoplastic models are also introduced. Each constitutive model is introduced with conceptual one-dimensional stress-strain models and then generalized for multidimensional stress space. Furthermore, each model is cast in an incremental form appropriate for numerical solution techniques. Finite element algorithms are outlined for incorporating these nonlinear constitutive models into boundary value problems. In particular, tangent stiffness and initial strain methods are discussed. The viscoelastic-plastic model is compared to experimental data for plexiglas and sea-ice to illustrate the versatility of the model in replicating nonlinear creep and flow of these materials. Moreover, the identification of the model parameters are shown to be no more difficult than the identification of parameters for the classical theories of plasticity and viscoelasticity.
- Published
- 1978
16. Experimental Relationships between Moduli For Soil Layers Beneath Concrete Pavements
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CIVIL ENGINEERING LAB (NAVY) PORT HUENEME CA, Forrest, J. B., Springston, P. S., Katona, M. G., Rollins, J., CIVIL ENGINEERING LAB (NAVY) PORT HUENEME CA, Forrest, J. B., Springston, P. S., Katona, M. G., and Rollins, J.
- Abstract
Two subgrades and four rigid pavement test sections were constructed and instrumented to recover experimental information of soil/rigid pavement behavior beneath static loadings. Pavement deflections and pavement/soil strain data were recorded for incremental static loads applied to a 30 inch diameter steel plate. Tests were conducted for comparison of experimentally collected data with Westergaard and elastic layer analyses of the test sections to investigate whether a consistent mathematical relationship exists between the two methods of analysis. To this end material characterizations were carried out for the component materials in the 4 different pavement sections and analytically predicted and experimentally obtained response data were compared.
- Published
- 1977
17. An Experimental Evaluation of a New Approach to Concrete Overlay Design for Flexible Pavements.
- Author
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NAVAL CIVIL ENGINEERING LAB PORT HUENEME CA, Nielsen, J. P., Katona, M. G., NAVAL CIVIL ENGINEERING LAB PORT HUENEME CA, Nielsen, J. P., and Katona, M. G.
- Abstract
The report is concerned with the design of concrete overlays for flexible pavements. The conventional Westergaard analysis for the design of concrete overlays is reviewed and its weaknesses are enumerated. A review of layered theory indicated that an elastic layered solid approach to overlay design would predict an overlay thickness thinner than that suggested by Westergaard and, thus it offers a dollar savings in construction costs without a loss in rated load capacity. A field test section consisting of overlays of different thicknesses was constructed and load tested to evaluate the appropriateness of the new theoretical approach and also to verify the assumed failure criterion. A limited strain concept was selected as the failure mode; that is, the maximum tensile strain in the concrete at the overlay-pavement interface should be limited to a prescribed maximum value. (Author)
- Published
- 1969
18. Ice-Engineering-Summary of Elastic Properties Research and Introduction to Viscoelastic and Nonlinear Analysis of Saline Ice.
- Author
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NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Katona,M. G., Vaudrey,K. D., NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Katona,M. G., and Vaudrey,K. D.
- Abstract
Increasing operational use od ice areas in polar regions has heightened the requirement for improved knowledge and techniques to analyze the behavior of sea ice. Previous studies on elastic behavior are reviewed with emphasis placed on plate analysis. Classical plate theory and the finite element method are compared in analyzing ice plates, with special attention given to sea-ice airfields. Since elastic analysis is not totally representative of actual ice behavior, a general formulation is presented which gives the assumptions and procedures for both viscoelastic and nonlinear domains of sea-ice behavior. A laboratory program is being initiated to determine material properties that are necessary to extend sea-ice analysis into the inelastic range. (Author)
- Published
- 1973
19. Layered Pavement Systems. Part I. Layered System Design. Part II. Fatigue of Plain Concrete.
- Author
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NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Forrest,J. B., Katona,M. G., Griffin,D. F., NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Forrest,J. B., Katona,M. G., and Griffin,D. F.
- Abstract
The report describes a recent Naval Civil Engineering Laboratory study of airfield pavement overlay design that indicates that elastic layered analysis may be a better design approach than any other currently available technique. A finite element theory of analysis has been developed that considers horizontal sliding between layers, and the superimposed load effects of multiple-wheel landing gear. This theory also provides for automatic finite element mesh generation, and automatic plotting of stress, strain, and displacement output data. The report also presents a review of the literature about fatigue of plain concrete. It reveals information about beam and cylinder testing but discloses no conclusive experimental work on the fatigue behavior of uniformly supported pavement slabs. Fatigue behavior estimates based upon beam and cylinder tests would necessarily have to be conservative and therefore self-defeating insofar as achieving economy of design by adoption of minimum feasible thickness of pavement slab overlays. (Author)
- Published
- 1972
20. STRUCTURAL DESIGN OF CONICAL ACRYLIC VIEWPORTS.
- Author
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NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Snoey,M. R., Katona,M. G., NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Snoey,M. R., and Katona,M. G.
- Abstract
A time-dependent, yield-failure criterion was developed and utilized in the analysis of a variety of viewport configurations. A RANGE OF THICKNESS/MINOR DIAMETER RATIOS FROM 50.25 TO 1.75 AND INCLUDED ANGLES FROM 60 DEGREE TO 120 DEGREE WERE ANALYZED BY THE FINITE ELEMENT TECHNIQUE. Using the viewport structural analysis in conjunction with the yield-failure criterion for acrylic, time- dependent operating depths were determined as a function of viewport configuration. An experimental investigation was performed to validate the analytical results. Six full- scale viewports were tested for a year under simulated operational conditions that included simultaneous cycling of pressure and temperature, 0 to 4,000 psi and 70 to 35 F, respectively. Comparison of analytical and experimental results indicated excellent agreement for the physical location of viewport failure at specified loading histories. Design recommendations are presented in the form of design curves which enable the design of a conical acrylic viewport for a specified operating pressure and duration under load. Design information is given also on sealing with a conventional 0-ring, as well as guidelines for elevating a viewport in its flange. (Author-PL)
- Published
- 1970
21. STRUCTURAL BEHAVIOR OF REINFORCED CONCRETE BEAMS MADE WITH FAST-FIX 1 CEMENT.
- Author
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NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Nosseir,S. B., Katona,M. G., NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Nosseir,S. B., and Katona,M. G.
- Abstract
The objective was to investigate the predictability and effectiveness of reinforced concrete beams made with Fast-Fix 1 cement. Fourteen simply supported beams were designed with the beam parameters systematically varied in order to observe a wide range of structural response when the beams were loaded to failure. The ultimate strength and mode of failure of test specimens were compared with predicted results based on the ultimate strength design assumptions of the ACl Code. In addition, 'elastic' behavior in terms of beam stiffness was compared to calculated stiffnesses based on the working stress design assumptions of the ACl Code. The performance of two Fast-Fix beams was also compared with the test results of 'duplicate' beams made with portland cement. (Author)
- Published
- 1969
22. MIX DESIGNS FOR FAST-FIX 1 CONCRETE.
- Author
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NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Nosseir,S. B., Katona,M. G., NAVAL CIVIL ENGINEERING LAB PORT HUENEME CALIF, Nosseir,S. B., and Katona,M. G.
- Abstract
The purpose of this investigation was to obtain information required for the use of Fast-Fix 1 concrete as a structural material. In particular, the effects of concrete mix design parameters and aging were investigated to determine if varying them would permit attaining compressive strengths in excess of 2,500 psi without the use of retarding agents. In addition, splitting tensile strength and compressive stress-strain relations were determined for standard control cylinders. Recommendations are presented in the form of design curves which enable one to design a mix for a specified compressive strength and set time. (Author)
- Published
- 1969
23. Structural Analysis of a Full-Scale Spherical Acrylic Plastic Pressure Hull.
- Author
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NAVAL CIVIL ENGINEERING LAB PORT HUENEME CA, Snoey, M. R., Katona, M. G., NAVAL CIVIL ENGINEERING LAB PORT HUENEME CA, Snoey, M. R., and Katona, M. G.
- Abstract
Analysis of a design of a pressure hull incorporating 12 spherical pentagons of acrylic plastic bonded together to form a sphere with an outside diameter of 66 inches and a wall thickness of 2.5 inches. Steel penetrations were located at the two poles. The experimental results were obtained from strain gage data from two independent pressure tests to 500 psi on two acrylic plastic hulls of the same design. The finite element analysis of the hull structure placed particular emphasis on the acrylic plastic-steel boundary. The boundary conditions at the acrylic plastic-steel interface were two extreme cases: fixed and free. A time-dependent yield-failure criterion for acrylic plastic was combined with the structural analysis to provide an operating depth curve as a function of both time and temperature. Comparison of the finite element and experimental results indicated excellent agreement. At a temperature of 70 F and a maximum of 50 hours load duration, the acrylic plastic hull can operate to 1,000 feet with a safety factor of 1.5 based on yield and a safety factor of 2.6 based on collapse. Design recommendations are also presented to provide guidelines for future design of the hull and its integration with an undersea vehicle. (Author, modified-PL)
- Published
- 1971
24. Modification of calcite and quartz grindability by surfactants.
- Author
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Farkas G., Fodor J., Katona M., Farkas G., Fodor J., and Katona M.
- Abstract
The strongly polar reagents, having an increased adsorption capacity on calcite (possibly chemical bond) produce a significant improvement in grindability. The reagent effect on quartz grinding is significantly lower than on calcite, owing to the weaker interaction between the solid surface and the reagent molecules., The strongly polar reagents, having an increased adsorption capacity on calcite (possibly chemical bond) produce a significant improvement in grindability. The reagent effect on quartz grinding is significantly lower than on calcite, owing to the weaker interaction between the solid surface and the reagent molecules.
25. CHLORPROMAZINE TREATMENT OF PERSISTENT PULMONARY HYPERTENSION IN THE NEUBORN
- Author
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Katona, M, primary, Hencz, P, additional, Kertesz, E, additional, Tekulics, P, additional, Beviz, J, additional, and Linderkamp, O, additional
- Published
- 1985
- Full Text
- View/download PDF
26. Systematic mapping of mitochondrial calcium uniporter channel (MCUC)-mediated calcium signaling networks.
- Author
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Delgado de la Herran H, Vecellio Reane D, Cheng Y, Katona M, Hosp F, Greotti E, Wettmarshausen J, Patron M, Mohr H, Prudente de Mello N, Chudenkova M, Gorza M, Walia S, Feng MS, Leimpek A, Mielenz D, Pellegata NS, Langer T, Hajnóczky G, Mann M, Murgia M, and Perocchi F
- Abstract
The mitochondrial calcium uniporter channel (MCUC) mediates mitochondrial calcium entry, regulating energy metabolism and cell death. Although several MCUC components have been identified, the molecular basis of mitochondrial calcium signaling networks and their remodeling upon changes in uniporter activity have not been assessed. Here, we map the MCUC interactome under resting conditions and upon chronic loss or gain of mitochondrial calcium uptake. We identify 89 high-confidence interactors that link MCUC to several mitochondrial complexes and pathways, half of which are associated with human disease. As a proof-of-concept, we validate the mitochondrial intermembrane space protein EFHD1 as a binding partner of the MCUC subunits MCU, EMRE, and MCUB. We further show a MICU1-dependent inhibitory effect of EFHD1 on calcium uptake. Next, we systematically survey compensatory mechanisms and functional consequences of mitochondrial calcium dyshomeostasis by analyzing the MCU interactome upon EMRE, MCUB, MICU1, or MICU2 knockdown. While silencing EMRE reduces MCU interconnectivity, MCUB loss-of-function leads to a wider interaction network. Our study provides a comprehensive and high-confidence resource to gain insights into players and mechanisms regulating mitochondrial calcium signaling and their relevance in human diseases., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
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27. Cytochrome b5 reductases: Redox regulators of cell homeostasis.
- Author
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Hall R, Yuan S, Wood K, Katona M, and Straub AC
- Subjects
- Humans, Cytochromes b5 metabolism, Oxidation-Reduction, Homeostasis, Cytochrome Reductases metabolism, Cytochrome-B(5) Reductase genetics, Cytochrome-B(5) Reductase metabolism, Methemoglobinemia congenital, Methemoglobinemia genetics
- Abstract
The cytochrome-b
5 reductase (CYB5R) family of flavoproteins is known to regulate reduction-oxidation (redox) balance in cells. The five enzyme members are highly compartmentalized at the subcellular level and function as "redox switches" enabling the reduction of several substrates, such as heme and coenzyme Q. Critical insight into the physiological and pathophysiological significance of CYB5R enzymes has been gleaned from several human genetic variants that cause congenital disease and a broad spectrum of chronic human diseases. Among the CYB5R genetic variants, CYB5R3 is well-characterized and deficiency in expression and activity is associated with type II methemoglobinemia, cancer, neurodegenerative disorders, diabetes, and cardiovascular disease. Importantly, pharmacological and genetic-based strategies are underway to target CYB5R3 to circumvent disease onset and mitigate severity. Despite our knowledge of CYB5R3 in human health and disease, the other reductases in the CYB5R family have been understudied, providing an opportunity to unravel critical function(s) for these enzymes in physiology and disease. In this review, we aim to provide the broad scientific community an up-to-date overview of the molecular, cellular, physiological, and pathophysiological roles of CYB5R proteins., Competing Interests: Conflict of interest Dr Straub is a consultant and stockholder for Creegh Pharmaceuticals. Dr Straub received research funds from Bayer Pharmaceuticals. All other authors declare that they have no conflicts of interest with the contents of this article., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2022
- Full Text
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28. Capture at the ER-mitochondrial contacts licenses IP 3 receptors to stimulate local Ca 2+ transfer and oxidative metabolism.
- Author
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Katona M, Bartók Á, Nichtova Z, Csordás G, Berezhnaya E, Weaver D, Ghosh A, Várnai P, Yule DI, and Hajnóczky G
- Subjects
- Inositol 1,4,5-Trisphosphate Receptors metabolism, Cell Respiration, Calcium metabolism, Oxidative Stress, Calcium Signaling physiology, Mitochondria metabolism
- Abstract
Endoplasmic reticulum-mitochondria contacts (ERMCs) are restructured in response to changes in cell state. While this restructuring has been implicated as a cause or consequence of pathology in numerous systems, the underlying molecular dynamics are poorly understood. Here, we show means to visualize the capture of motile IP
3 receptors (IP3Rs) at ERMCs and document the immediate consequences for calcium signaling and metabolism. IP3Rs are of particular interest because their presence provides a scaffold for ERMCs that mediate local calcium signaling, and their function outside of ERMCs depends on their motility. Unexpectedly, in a cell model with little ERMC Ca2+ coupling, IP3Rs captured at mitochondria promptly mediate Ca2+ transfer, stimulating mitochondrial oxidative metabolism. The Ca2+ transfer does not require linkage with a pore-forming protein in the outer mitochondrial membrane. Thus, motile IP3Rs can traffic in and out of ERMCs, and, when 'parked', mediate calcium signal propagation to the mitochondria, creating a dynamic arrangement that supports local communication., (© 2022. The Author(s).)- Published
- 2022
- Full Text
- View/download PDF
29. Comparative Study of the Solid-Liquid Interfacial Adsorption of Proteins in Their Native and Amyloid Forms.
- Author
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Ábrahám Á, Massignan F, Gyulai G, Katona M, Taricska N, and Kiss É
- Subjects
- Adsorption, Microscopy, Atomic Force methods, Hydrophobic and Hydrophilic Interactions, Amyloidogenic Proteins, Surface Properties, Quartz Crystal Microbalance Techniques, Amyloid
- Abstract
The adhesive properties of amyloid fibers are thought to play a crucial role in various negative and positive aggregation processes, the study of which might help in their understanding and control. Amyloids have been prepared from two proteins, lysozyme and β-lactoglobulin, as well as an Exendin-4 derivative miniprotein (E5). Thermal treatment was applied to form amyloids and their structure was verified by thioflavin T (ThT), 8-Anilino-1-naphthalenesulfonic acid (ANS) dye tests and electronic circular dichroism spectroscopy (ECD). Adsorption properties of the native and amyloid forms of the three proteins were investigated and compared using the mass-sensitive quartz crystal microbalance (QCM) technique. Due to the possible electrostatic and hydrophobic interactions, similar adsorbed amounts were found for the native or amyloid forms, while the structures of the adsorbed layers differed significantly. Native proteins formed smooth and dense adsorption layers. On the contrary, a viscoelastic, highly loose layer was formed in the presence of the amyloid forms, shown by increased motional resistance values determined by the QCM technique and also indicated by atomic force microscopy (AFM) and wettability measurements. The elongated structure and increased hydrophobicity of amyloids might contribute to this kind of aggregation.
- Published
- 2022
- Full Text
- View/download PDF
30. Seroprevalence of Four Polyomaviruses Linked to Dermatological Diseases: New Findings and a Comprehensive Analysis.
- Author
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Jeles K, Katona M, and Csoma E
- Subjects
- Humans, Adult, Aged, Middle Aged, Infant, Child, Preschool, Child, Adolescent, Young Adult, Aged, 80 and over, Seroepidemiologic Studies, Capsid Proteins genetics, Immunoglobulin G, Polyomavirus genetics, Polyomavirus Infections epidemiology, Merkel cell polyomavirus genetics, Tumor Virus Infections epidemiology
- Abstract
Our aim was to study the seroprevalence of human polyomaviruses (HPyV) linked to skin diseases. A total of 552 serum samples were analysed by the enzyme-linked immunosorbent assay to detect IgG antibodies against Merkel cell polyomavirus (MCPyV), HPyV6, HPyV7 and Trichodysplasia spinulosa-associated polyomavirus (TSPyV) using recombinant major capsid proteins of these viruses. The individuals (age 0.8−85 years, median 33) were sorted into seven age groups: <6, 6−10, 10−14, 14−21, 21−40, 40−60 and >60 years. The adulthood seroprevalence was 69.3%, 87.7%, 83.8% and 85% for MCPyV, HPyV6, HPyV7 and TSPyV, respectively. For all four polyomaviruses, there was increasing seropositivity with age until reaching the adulthood level. There was a significant increase in seroreactivity for those age groups in which the rate of already-infected individuals also showed significant differences. The adulthood seropositvity was relatively stable with ageing, except for TSPyV, for which elevated seropositivity was observed for the elderly (>60 years) age group. Since seroepidemiological data have been published with wide ranges for all the viruses studied, we performed a comprehensive analysis comparing the published age-specific seropositivities to our data. Although the cohorts, methods and even the antigens were variable among the studies, there were similar results for all studied polyomaviruses. For MCPyV, geographically distinct genotypes might exist, which might also result in the differences in the seroprevalence data. Additional studies with comparable study groups and methods are required to clarify whether there are geographical differences.
- Published
- 2022
- Full Text
- View/download PDF
31. Riociguat in children with pulmonary arterial hypertension: The PATENT-CHILD study.
- Author
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García Aguilar H, Gorenflo M, Ivy DD, Moledina S, Castaldi B, Ishida H, Cześniewicz P, Kusa J, Miera O, Pattathu J, Weng KP, Ablonczy L, Apitz C, Katona M, Kurosaki K, Pulido T, Yamagishi H, Yasuda K, Cisternas G, Goth M, Lippert S, Radomskyj A, Saleh S, Willmann S, Wirsching G, Bonnet D, and Beghetti M
- Abstract
Riociguat, a soluble guanylate cyclase stimulator, is approved for treatment of adults with pulmonary arterial hypertension (PAH). The safety, tolerability, and pharmacokinetics (PK) of oral riociguat in a pediatric population with PAH was assessed in PATENT-CHILD (NCT02562235), a multicenter, single-arm, 24-week, open-label, Phase 3 study. Patients aged 6-17 years in World Health Organization functional class (WHO-FC) I-III treated with stable endothelin receptor antagonists and/or prostacyclin analogs received riociguat equivalent to 0.5-2.5 mg three times daily in adults, as either oral pediatric suspension or tablets, based on bodyweight. Primary outcomes were safety, tolerability, and PK of riociguat. Twenty-four patients (mean age 12.8 years), 18 of whom were in WHO-FC II, were enrolled. Adverse events (AEs), mostly mild or moderate, were reported in 20 patients (83%). Four patients (17%) experienced a serious AE; all resolved by study end and two (8%) were considered study-drug related. Hypotension was reported in three patients and hemoptysis in one (all mild/moderate intensity). Riociguat plasma concentrations in pediatric patients were consistent with those published in adult patients. From baseline to Week 24, mean ± standard deviation increase in 6-minute walking distance was 23 ± 69 m ( n = 19), and mean decrease in NT-proBNP was -66 ± 585 pg/ml ( n = 14). There was no change in WHO-FC. Two patients experienced clinical worsening events of hospitalization for right heart failure. PK results confirmed a suitable riociguat dosing strategy for pediatric patients with PAH. The data suggest an acceptable safety profile with potential efficacy signals., Competing Interests: H. G. A. reports fees for lectures and member of advisory boards for Bayer and Janssen. D. D. I. reports the University of Colorado contracts with Acceleron, Actelion/Janssen, Altavant, Bayer, Eli Lilly, GlaxoSmithKline, and United Therapeutics for D. D. I. to be a consultant and perform clinical trials. S. M. reports consultancy from Actelion/Janssen and GlaxoSmithKline. J. P. reports fees for lectures and/or consultancy from Actelion/Janssen. T. P. reports fees for lectures, member of advisory boards, and research grants from Actelion/Janssen, Bayer, GlaxoSmithKline, and Pfizer. H. Y. reports research grants and lecture fees from Actelion, AbbVie, and Teijin Pharma; research grants from Tanabe‐Mitsubishi; lecture fees from Bayer, GE Healthcare Japan, Merck Sharp and Dohme, Novo Nordisk, Nihon Seiyaku, Nihon Shinyaku, Pfizer, and Termo. G. C., M. G., S. L., A. R., S. S., S. W., G. W. are employees and stockholders of Bayer AG. D. B. is a steering committee member for Eli Lilly and Company, and steering committee and advisory board member for Actelion Pharmaceuticals, Bayer, and Novartis. M. B. reports fees for lectures and/or consultancy from Acceleron, Actelion/Janssen, AOP, Bayer, Eli Lilly, GlaxoSmithKline, and Gossamer; unrestricted research grants from Actelion and Bayer. The remaining authors declare no conflict of interest., (© 2022 The Authors. Pulmonary Circulation published by John Wiley & Sons Ltd on behalf of Pulmonary Vascular Research Institute.)
- Published
- 2022
- Full Text
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32. KI and WU Polyomaviruses: Seroprevalence Study and DNA Prevalence in SARS-CoV-2 RNA Positive and Negative Respiratory Samples.
- Author
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Katona M, Jeles K, Kovács R, and Csoma E
- Abstract
The aim of this work was to study the possible co-infection of KI and WU polyomavirus (KIPyV and WUPyV, respectively) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in respiratory samples and to detect the seroprevalence of KIPyV and WUPyV. A total of 1030 nasopharyngeal samples were analyzed from SARS-CoV-2 RNA positive ( n = 680) and negative ( n = 350) adults and children (age: 1 day to 94.2 years) collected from August 2020 to October 2021. KIPyV DNA was detected in two SARS-CoV-2-positive samples (2/680, 0.29%) and in three SARS-CoV-2-negative samples (3/350, 0.86%). WUPyV DNA was observed in one-one samples from both groups (1/680, 0.15% vs. 1/350, 0.29%). We did not find an association between SARS-CoV-2 and KIPyV or WUPyV infection, and we found low DNA prevalence of polyomaviruses studied after a long-term lockdown in Hungary. To exclude a geographically different distribution of these polyomaviruses, we studied the seroprevalence of KIPyV and WUPyV by enzyme-linked immunosorbent assay among children and adults ( n = 692 for KIPyV and n = 705 for WUPyV). Our data confirmed that primary infections by KIPyV and WUPyV occur mainly during childhood; the overall seropositivity of adults was 93.7% and 89.2% for KIPyV and WUPyV, respectively. Based on our data, we suggest that the spread of KIPyV and WUPyV might have been restricted in Hungary by the lockdown.
- Published
- 2022
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33. Systemic Screening for 22q11.2 Copy Number Variations in Hungarian Pediatric and Adult Patients With Congenital Heart Diseases Identified Rare Pathogenic Patterns in the Region.
- Author
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Zodanu GKE, Oszlánczi M, Havasi K, Kalapos A, Rácz G, Katona M, Ujfalusi A, Nagy O, Széll M, and Nagy D
- Abstract
Congenital heart defects (CHD) are the most common developmental abnormalities, affecting approximately 0.9% of livebirths. Genetic factors, including copy number variations (CNVs), play an important role in their development. The most common CNVs are found on chromosome 22q11.2. The genomic instability of this region, caused by the eight low copy repeats (LCR A-H), may result in several recurrent and/or rare microdeletions and duplications, including the most common, ∼3 Mb large LCR A-D deletion (classical 22q.11.2 deletion syndrome). We aimed to screen 22q11.2 CNVs in a large Hungarian pediatric and adult CHD cohort, regardless of the type of their CHDs. All the enrolled participants were cardiologically diagnosed with non-syndromic CHDs. A combination of multiplex ligation-dependent probe amplification (MLPA), chromosomal microarray analysis and droplet digital PCR methods were used to comprehensively assess the detected 22q11.2 CNVs in 212 CHD-patients. Additionally, capillary sequencing was performed to detect variants in the TBX1 gene, a cardinal gene located in 22q11.2. Pathogenic CNVs were detected in 5.2% (11/212), VUS in 0.9% and benign CNVs in 1.8% of the overall CHD cohort. In patients with tetralogy of Fallot the rate of pathogenic CNVs was 17% (5/30). Fifty-four percent of all CNVs were typical proximal deletions (LCR A-D). However, nested (LCR A-B) and central deletions (LCR C-D), proximal (LCR A-D) and distal duplications (LCR D-E, LCR D-H, LCR E-H, LCR F-H) and rare combinations of deletions and duplications were also identified. Segregation analysis detected familial occurrence in 18% (2/11) of the pathogenic variants. Based on in-depth clinical information, a detailed phenotype-genotype comparison was performed. No pathogenic variant was identified in the TBX1 gene. Our findings confirmed the previously described large phenotypic diversity in the 22q11.2 CNVs. MLPA proved to be a highly efficient genetic screening method for our CHD-cohort. Our results highlight the necessity for large-scale genetic screening of CHD-patients and the importance of early genetic diagnosis in their clinical management., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Zodanu, Oszlánczi, Havasi, Kalapos, Rácz, Katona, Ujfalusi, Nagy, Széll and Nagy.)
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- 2021
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34. Two Classes of T1 Hypointense Lesions in Multiple Sclerosis With Different Clinical Relevance.
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Kocsis K, Szabó N, Tóth E, Király A, Faragó P, Kincses B, Veréb D, Bozsik B, Boross K, Katona M, Bodnár P, László NG, Vécsei L, Klivényi P, Bencsik K, and Kincses ZT
- Abstract
Background: Hypointense lesions on T1-weighted images have important clinical relevance in multiple sclerosis patients. Traditionally, spin-echo (SE) sequences are used to assess these lesions (termed black holes), but Fast Spoiled Gradient-Echo (FSPGR) sequences provide an excellent alternative. Objective: To determine whether the contrast difference between T1 hypointense lesions and the surrounding normal white matter is similar on the two sequences, whether different lesion types could be identified, and whether the clinical relevance of these lesions types are different. Methods: Seventy-nine multiple sclerosis patients' lesions were manually segmented, then registered to T1 sequences. Median intensity values of lesions were identified on all sequences, then K-means clustering was applied to assess whether distinct clusters of lesions can be defined based on intensity values on SE, FSPGR, and FLAIR sequences. The standardized intensity of the lesions in each cluster was compared to the intensity of the normal appearing white matter in order to see if lesions stand out from the white matter on a given sequence. Results: 100% of lesions on FSPGR images and 69% on SE sequence in cluster #1 exceeded a standardized lesion distance of Z = 2.3 ( p < 0.05). In cluster #2, 78.7% of lesions on FSPGR and only 17.7% of lesions on SE sequence were above this cutoff value, meaning that these lesions were not easily seen on SE images. Lesion count in the second cluster (lesions less identifiable on SE) significantly correlated with the Expanded Disability Status Scale (EDSS) ( R : 0.30, p ≤ 0.006) and with disease duration ( R : 0.33, p ≤ 0.002). Conclusion: We showed that black holes can be separated into two distinct clusters based on their intensity values on various sequences, only one of which is related to clinical parameters. This emphasizes the joint role of FSPGR and SE sequences in the monitoring of MS patients and provides insight into the role of black holes in MS., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Kocsis, Szabó, Tóth, Király, Faragó, Kincses, Veréb, Bozsik, Boross, Katona, Bodnár, László, Vécsei, Klivényi, Bencsik and Kincses.)
- Published
- 2021
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35. On Absorption Modeling and Food Effect Prediction of Rivaroxaban, a BCS II Drug Orally Administered as an Immediate-Release Tablet.
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Kushwah V, Arora S, Tamás Katona M, Modhave D, Fröhlich E, and Paudel A
- Abstract
The present work evaluates the food effect on the absorption of rivaroxaban (Riva), a BCS II drug, from the orally administered commercial immediate-release tablet (Xarelto IR) using physiologically based pharmacokinetic (PBPK) and conventional in vitro-in vivo correlation (IVIVC) models. The bioavailability of Riva upon oral administration of Xarelto IR tablet is reported to exhibit a positive food effect. The PBPK model for Riva was developed and verified using the previously reported in vivo data for oral solution (5 and 10 mg) and Xarelto IR tablet (5 and 10 mg dose strength). Once the PBPK model was established, the in vivo performance of the tablet formulation with the higher dose strength (Xarelto IR tablet 20 mg in fasted and fed state) was predicted using the experimentally obtained data of in vitro permeability, biorelevant solubility and in vitro dynamic dissolution data using United States Pharmacopeia (USP) IV flow-through cell apparatus. In addition, the mathematical IVIVC model was developed using the in vitro dissolution and in vivo profile of 20 mg strength Xarelto IR tablet in fasted condition. Using the developed IVIVC model, the pharmacokinetic (PK) profile of the Xarelto IR tablet in fed condition was predicted and compared with the PK parameters obtained via the PBPK model. A virtual in vivo PK study was designed using a single-dose, 3-treatment cross-over trial in 50 subjects to predict the PK profile of the Xarelto® IR tablet in the fed state. Overall, the results obtained from the IVIVC model were found to be comparable with those from the PBPK model. The outcome from both models pointed to the positive food effect on the in vivo profile of the Riva. The developed models thus can be effectively extended to establish bioequivalence for the marketed and novel complex formulations of Riva such as amorphous solid dispersions.
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- 2021
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36. IP 3 receptor isoforms differently regulate ER-mitochondrial contacts and local calcium transfer.
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Bartok A, Weaver D, Golenár T, Nichtova Z, Katona M, Bánsághi S, Alzayady KJ, Thomas VK, Ando H, Mikoshiba K, Joseph SK, Yule DI, Csordás G, and Hajnóczky G
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- Animals, Cell Line, Tumor, Cell Survival physiology, Chickens, HeLa Cells, Humans, Inositol 1,4,5-Trisphosphate Receptors genetics, Protein Isoforms genetics, Calcium Channels metabolism, Calcium Signaling physiology, Endoplasmic Reticulum metabolism, Inositol 1,4,5-Trisphosphate Receptors metabolism, Mitochondria metabolism
- Abstract
Contact sites of endoplasmic reticulum (ER) and mitochondria locally convey calcium signals between the IP
3 receptors (IP3R) and the mitochondrial calcium uniporter, and are central to cell survival. It remains unclear whether IP3Rs also have a structural role in contact formation and whether the different IP3R isoforms have redundant functions. Using an IP3R-deficient cell model rescued with each of the three IP3R isoforms and an array of super-resolution and ultrastructural approaches we demonstrate that IP3Rs are required for maintaining ER-mitochondrial contacts. This role is independent of calcium fluxes. We also show that, while each isoform can support contacts, type 2 IP3R is the most effective in delivering calcium to the mitochondria. Thus, these studies reveal a non-canonical, structural role for the IP3Rs and direct attention towards the type 2 IP3R that was previously neglected in the context of ER-mitochondrial calcium signaling.- Published
- 2019
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37. Novel Insight Into the Role of CFTR in Lacrimal Gland Duct Function in Mice.
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Berczeli O, Vizvári E, Katona M, Török D, Szalay L, Rárosi F, Németh I, Rakonczay Z, Hegyi P, Ding C, and Tóth-Molnár E
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- Animals, Biological Transport, Cells, Cultured, Dry Eye Syndromes pathology, Lacrimal Apparatus pathology, Mice, Mice, Inbred CFTR, Cystic Fibrosis Transmembrane Conductance Regulator metabolism, Dry Eye Syndromes metabolism, Lacrimal Apparatus metabolism, Tears metabolism
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Purpose: The role of cystic fibrosis transmembrane conductance regulator (CFTR) in lacrimal gland (LG) function has only recently received some attention, mainly from our group. In the present study, we investigated the potential changes of LG pathology, tear secretion, ocular surface integrity, and fluid secretion in isolated LG ducts from CFTR knockout (KO) mice., Methods: Tear production and ocular surface integrity were investigated in anesthetized wild-type (WT) and KO mice using cotton threads and fluorescein staining, respectively. Immunofluorescence was used to localize CFTR protein in the LGs. Ductal fluid secretions evoked by forskolin (10 μM); cell-permeable cAMP analogue (8-bromo cAMP, 100 μM); or carbachol (100 μM) were measured in isolated LG ducts using video-microscopy. Intracellular Ca2+ homeostasis underlying carbachol stimulation was investigated with microfluorometry., Results: Significant decrease in tear secretion and impaired ocular surface integrity were observed in KO mice. Immunofluorescence demonstrated the predominant presence of CFTR protein in the apical membranes of the duct cells from WT mice. Continuous fluid secretion was evoked by forskolin and 8-bromo cAMP in LG ducts from WT mice, while no secretory response was observed in ducts from KO mice. Carbachol caused similar secretory responses in ducts from WT and KO animals without significant differences in cytosolic Ca2+ signaling., Conclusions: Our results suggest the important role of CFTR in LG ductal secretion and in the maintenance of ocular surface integrity, suggesting that CFTR may be a promising target of novel therapeutic approaches in the treatment of dry eye.
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- 2018
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38. Different patterns of left ventricular "rigid body rotation" in 8-year-old twins with anamnestic twin-to-twin transfusion syndrome (from the MAGYAR-Twin Study).
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Nemes A, Katona M, Domsik P, Kalapos A, and Forster T
- Abstract
Competing Interests: Conflicts of Interest: The authors have no conflicts of interest to declare.
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- 2017
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39. Hyperspectral LED models for optical simulations.
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Rotscholl I, Katona M, Trampert K, Krüger U, Schmidt F, and Neumann C
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Precise spectral and colorimetric simulations in commercial ray tracing software require realistic light source models, which provide spectral information as a function of angle and spatial dimension. We describe and validate a general workflow to create hyperspectral LED models as a linear combination of spectral models. The workflow only requires user defined precisions and rayfiles obtained with different optical filters. The rayfiles are transformed into histogram based models, whose precision is evaluated by normalized cross-correlation values of their intensity distributions in the near-, mid- and far field. Additionally, the concept is evaluated with a spatial and spectral well defined test device.
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- 2016
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40. Characterization of Na+-K+-2Cl- Cotransporter Activity in Rabbit Lacrimal Gland Duct Cells.
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Vizvári E, Katona M, Orvos P, Berczeli O, Facskó A, Rárosi F, Venglovecz V, Rakonczay Z Jr, Hegyi P, Ding C, and Tóth-Molnár E
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- Analysis of Variance, Animals, Carbachol pharmacology, Carcinogens pharmacology, Colforsin pharmacology, Cyclic GMP analogs & derivatives, Cyclic GMP pharmacology, Dry Eye Syndromes etiology, Dry Eye Syndromes metabolism, Hydrogen-Ion Concentration, In Vitro Techniques, Male, Ophthalmic Solutions pharmacology, Osmolar Concentration, Rabbits, Solute Carrier Family 12, Member 2 metabolism, Tears metabolism, Tetradecanoylphorbol Acetate pharmacology, Vasoactive Intestinal Peptide pharmacology, Lacrimal Apparatus metabolism, Solute Carrier Family 12, Member 2 physiology
- Abstract
Purpose: We recently reported that isolated duct segments from rabbit lacrimal gland (LG) were able to secrete fluid in response to secretagogues, which were blocked completely by bumetanide. This suggests the functional involvement of Na+-K+-2Cl- cotransporter (NKCC1) in ductal fluid secretion. Therefore, the aim of this study was to investigate the activity profile of NKCC1 in isolated rabbit LG duct segments., Methods: Interlobular ducts were isolated from fresh rabbit LG tissue. Microfluorometry with the ammonium (NH4+)-pulse technique was used to elicit pH changes in duct cells, and the rate of bumetanide-sensitive cytosolic acidification after addition of NH4+ was used to quantify the activity of NKCC1., Results: While basal activity of NKCC1 was undetectable, low cytosolic chloride (Cl-) level and hyperosmotic challenge (390 mOsm) were able to increase the activity of NKCC1. Carbachol (100 μM) had no significant effect on NKCC1 activity. Elevation of cytosolic calcium (Ca2+) level with Ca2+-ionophore (A 23187, 1 μM) did not cause any alteration in the activity of the cotransporter while direct activation of protein kinase C (phorbol myristate acetate, 100 nM) increased its activity slightly but in a significant manner. Addition of either forskolin (10 μM), cell-permeable cAMP analogue (8-bromo cAMP, 100 μM) or vasoactive intestinal peptide (200 nM) resulted in a significant increase in the activity of NKCC1., Conclusions: These results highlight the functional involvement of NKCC1 in LG duct secretion. These findings may facilitate our understanding of LG function and may contribute to the development of targeted pharmacologic interventions in case of dry eye disease.
- Published
- 2016
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41. Role of ion transporters in the bile acid-induced esophageal injury.
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Laczkó D, Rosztóczy A, Birkás K, Katona M, Rakonczay Z Jr, Tiszlavicz L, Róka R, Wittmann T, Hegyi P, and Venglovecz V
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- Aged, Aged, 80 and over, Barrett Esophagus pathology, Bile Acids and Salts metabolism, Calcium metabolism, Cell Line, Chloride-Bicarbonate Antiporters metabolism, Epithelial Cells metabolism, Epithelial Cells pathology, Esophageal Mucosa metabolism, Esophageal Mucosa pathology, Female, Gene Expression Regulation, Humans, Hydrogen-Ion Concentration, Inositol Phosphates metabolism, Ion Transport, Male, Membrane Transport Proteins genetics, Metaplasia, Middle Aged, Sodium-Bicarbonate Symporters metabolism, Sodium-Hydrogen Exchangers metabolism, Time Factors, Barrett Esophagus metabolism, Bile Acids and Salts toxicity, Epithelial Cells drug effects, Esophageal Mucosa drug effects, Membrane Transport Proteins metabolism
- Abstract
Barrett's esophagus (BE) is considered to be the most severe complication of gastro-esophageal reflux disease (GERD), in which the prolonged, repetitive episodes of combined acidic and biliary reflux result in the replacement of the squamous esophageal lining by columnar epithelium. Therefore, the acid-extruding mechanisms of esophageal epithelial cells (EECs) may play an important role in the defense. Our aim was to identify the presence of acid/base transporters on EECs and to investigate the effect of bile acids on their expressions and functions. Human EEC lines (CP-A and CP-D) were acutely exposed to bile acid cocktail (BAC) and the changes in intracellular pH (pHi) and Ca(2+) concentration ([Ca(2+)]i) were measured by microfluorometry. mRNA and protein expression of ion transporters was investigated by RT-PCR, Western blot, and immunohistochemistry. We have identified the presence of a Na(+)/H(+) exchanger (NHE), Na(+)/HCO3 (-) cotransporter (NBC), and a Cl(-)-dependent HCO3 (-) secretory mechanism in CP-A and CP-D cells. Acute administration of BAC stimulated HCO3 (-) secretion in both cell lines and the NHE activity in CP-D cells by an inositol triphosphate-dependent calcium release. Chronic administration of BAC to EECs increased the expression of ion transporters compared with nontreated cells. A similar expression pattern was observed in biopsy samples from BE compared with normal epithelium. We have shown that acute administration of bile acids differently alters ion transport mechanisms of EECs, whereas chronic exposure to bile acids increases the expression of acid/base transporters. We speculate that these adaptive processes of EECs represent an important mucosal defense against the bile acid-induced epithelial injury., (Copyright © 2016 the American Physiological Society.)
- Published
- 2016
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42. A novel, protective role of ursodeoxycholate in bile-induced pancreatic ductal injury.
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Katona M, Hegyi P, Kui B, Balla Z, Rakonczay Z Jr, Rázga Z, Tiszlavicz L, Maléth J, and Venglovecz V
- Subjects
- Adenosine Triphosphate metabolism, Animals, Apoptosis drug effects, Calcium Signaling drug effects, Cell Death drug effects, Epithelial Cells drug effects, Guinea Pigs, Hydrogen-Ion Concentration, In Vitro Techniques, Male, Membrane Potential, Mitochondrial drug effects, Mitochondria drug effects, Rats, Rats, Sprague-Dawley, Bile Acids and Salts, Chenodeoxycholic Acid, Gastrointestinal Agents therapeutic use, Pancreatic Ducts injuries, Pancreatitis chemically induced, Pancreatitis prevention & control, Ursodeoxycholic Acid therapeutic use
- Abstract
We have previously shown that chenodeoxycholic acid (CDCA) strongly inhibits pancreatic ductal HCO3 (-) secretion through the destruction of mitochondrial function, which may have significance in the pathomechanism of acute pancreatitis (AP). Ursodeoxycholic acid (UDCA) is known to protect the mitochondria against hydrophobic bile acids and has an ameliorating effect on cell death. Therefore, our aim was to investigate the effect of UDCA pretreatment on CDCA-induced pancreatic ductal injury. Guinea pig intrainterlobular pancreatic ducts were isolated by collagenase digestion. Ducts were treated with UDCA for 5 and 24 h, and the effect of CDCA on intracellular Ca(2+) concentration ([Ca(2+)]i), intracellular pH (pHi), morphological and functional changes of mitochondria, and the rate of apoptosis were investigated. AP was induced in rat by retrograde intraductal injection of CDCA (0.5%), and the disease severity of pancreatitis was assessed by measuring standard laboratory and histological parameters. Twenty-four-hour pretreatment of pancreatic ducts with 0.5 mM UDCA significantly reduced the rate of ATP depletion, mitochondrial injury, and cell death induced by 1 mM CDCA and completely prevented the inhibitory effect of CDCA on acid-base transporters. UDCA pretreatment had no effect on CDCA-induced Ca(2+) signaling. Oral administration of UDCA (250 mg/kg) markedly reduced the severity of CDCA-induced AP. Our results clearly demonstrate that UDCA 1) suppresses the CDCA-induced pancreatic ductal injury by reducing apoptosis and mitochondrial damage and 2) reduces the severity of CDCA-induced AP. The protective effect of UDCA against hydrophobic bile acids may represent a novel therapeutic target in the treatment of biliary AP., (Copyright © 2016 the American Physiological Society.)
- Published
- 2016
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43. Predictors of health-related quality of life in stroke patients after neurological inpatient rehabilitation: a prospective study.
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Katona M, Schmidt R, Schupp W, and Graessel E
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- Aged, Cohort Studies, Female, Follow-Up Studies, Forecasting, Humans, Male, Middle Aged, Multivariate Analysis, Prospective Studies, Regression Analysis, Surveys and Questionnaires, Health Status, Outcome Assessment, Health Care trends, Quality of Life psychology, Stroke psychology, Stroke Rehabilitation, Survivors psychology
- Abstract
Background: The goal of the study was to investigate the long-term course of health-related quality of life (HRQoL) in stroke survivors during and up to 2.5 years after inpatient neurological rehabilitation and to identify predictors of HRQoL., Methods: HRQoL was determined in 152 stroke survivors in a single-centre prospective cohort study at four time points: upon admission to inpatient rehabilitation, at discharge, and one and 2.5 years after discharge. Their HRQoL was determined by administering the EQ-5D at all four measurement points. During inpatient rehabilitation, the SF-36 was administered in addition to the EQ-5D. Predictors were identified through multiple regression analysis., Results: During inpatient rehabilitation, the "European Index" of the EQ-5D rose significantly (p < 0.001) from 45.4 to 66.7. The change in HRQoL on the SF-36 was convergent. The HRQoL of the stroke patients living at home remained at the same level for 2.5 years following discharge. In the multiple regression analysis, the EQ-5D Index at discharge (p = 0.049), the risk of falls as defined by Runge and Rehfeld (p = 0.001), and the change in emotional quality of life on the SF-36 during inpatient rehabilitation (p = 0.048) predicted HRQoL 2.5 years following discharge., Conclusion: On the basis of our results, we conclude that the long-term health-related quality of life of stroke survivors can be positively influenced by reducing the risk of falls and improving emotional well-being during neurological inpatient rehabilitation.
- Published
- 2015
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44. Alcohol disrupts levels and function of the cystic fibrosis transmembrane conductance regulator to promote development of pancreatitis.
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Maléth J, Balázs A, Pallagi P, Balla Z, Kui B, Katona M, Judák L, Németh I, Kemény LV, Rakonczay Z Jr, Venglovecz V, Földesi I, Pető Z, Somorácz Á, Borka K, Perdomo D, Lukacs GL, Gray MA, Monterisi S, Zaccolo M, Sendler M, Mayerle J, Kühn JP, Lerch MM, Sahin-Tóth M, and Hegyi P
- Subjects
- Adenosine Triphosphate analysis, Animals, Bicarbonates metabolism, Calcium metabolism, Chloride Channels antagonists & inhibitors, Cystic Fibrosis Transmembrane Conductance Regulator analysis, Cystic Fibrosis Transmembrane Conductance Regulator genetics, Guinea Pigs, Humans, Mice, Mutation, Protein Folding drug effects, Cystic Fibrosis Transmembrane Conductance Regulator physiology, Ethanol toxicity, Pancreatitis chemically induced
- Abstract
Background & Aims: Excessive consumption of ethanol is one of the most common causes of acute and chronic pancreatitis. Alterations to the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR) also cause pancreatitis. However, little is known about the role of CFTR in the pathogenesis of alcohol-induced pancreatitis., Methods: We measured CFTR activity based on chloride concentrations in sweat from patients with cystic fibrosis, patients admitted to the emergency department because of excessive alcohol consumption, and healthy volunteers. We measured CFTR levels and localization in pancreatic tissues and in patients with acute or chronic pancreatitis induced by alcohol. We studied the effects of ethanol, fatty acids, and fatty acid ethyl esters on secretion of pancreatic fluid and HCO3(-), levels and function of CFTR, and exchange of Cl(-) for HCO3(-) in pancreatic cell lines as well as in tissues from guinea pigs and CFTR knockout mice after administration of alcohol., Results: Chloride concentrations increased in sweat samples from patients who acutely abused alcohol but not in samples from healthy volunteers, indicating that alcohol affects CFTR function. Pancreatic tissues from patients with acute or chronic pancreatitis had lower levels of CFTR than tissues from healthy volunteers. Alcohol and fatty acids inhibited secretion of fluid and HCO3(-), as well as CFTR activity, in pancreatic ductal epithelial cells. These effects were mediated by sustained increases in concentrations of intracellular calcium and adenosine 3',5'-cyclic monophosphate, depletion of adenosine triphosphate, and depolarization of mitochondrial membranes. In pancreatic cell lines and pancreatic tissues of mice and guinea pigs, administration of ethanol reduced expression of CFTR messenger RNA, reduced the stability of CFTR at the cell surface, and disrupted folding of CFTR at the endoplasmic reticulum. CFTR knockout mice given ethanol or fatty acids developed more severe pancreatitis than mice not given ethanol or fatty acids., Conclusions: Based on studies of human, mouse, and guinea pig pancreata, alcohol disrupts expression and localization of the CFTR. This appears to contribute to development of pancreatitis. Strategies to increase CFTR levels or function might be used to treat alcohol-associated pancreatitis., (Copyright © 2015 AGA Institute. Published by Elsevier Inc. All rights reserved.)
- Published
- 2015
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45. Experimental evidence of fluid secretion of rabbit lacrimal gland duct epithelium.
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Katona M, Vizvári E, Németh L, Facskó A, Venglovecz V, Rakonczay Z Jr, Hegyi P, and Tóth-Molnár E
- Subjects
- Animals, Atropine pharmacology, Biological Transport, Body Fluids drug effects, Carbachol pharmacology, Cholinergic Agonists pharmacology, Epithelium drug effects, Epithelium metabolism, Hydrogen-Ion Concentration, Male, Muscarinic Antagonists pharmacology, Nasolacrimal Duct cytology, Nasolacrimal Duct drug effects, Osmolar Concentration, Permeability drug effects, Rabbits, Tissue Culture Techniques, Water metabolism, Body Fluids metabolism, Nasolacrimal Duct metabolism
- Abstract
Purpose: To investigate the osmotic water permeability of lacrimal gland (LG) duct epithelium by means of calculation of filtration permeability and to investigate LG ductal fluid secretion., Methods: Experiments were performed on isolated rabbit LG duct segments maintained in short-term culture. Osmotically determined fluid movement or fluid secretion into the closed intraluminal space of cultured LG interlobular ducts was analyzed using video microscopic technique., Results: The end of the LG ducts sealed after overnight incubation forming a closed luminal space. For the calculation of osmotic water permeability, ducts were initially perfused with isotonic HEPES buffered solution, and then with hypotonic HEPES buffered solution. Filtration permeability was calculated from the initial slope of the relative volume increase. Secretory responses to carbachol or to forskolin stimulation were also investigated. Forskolin stimulation resulted in a rapid and sustained secretory response in both solutions. Forskolin-stimulated fluid secretion was completely inhibited by bumetanide both in HEPES buffered and in HCO3 (-)/CO2 buffered solutions, suggesting the central role of Na(+)-K(+)-2Cl(-) cotransporter type 1 (NKCC1). Administration of carbachol initiated a rapid but short secretory response in both HEPES buffered and in HCO3 (-)/CO2 buffered solutions. Atropine completely abolished the carbachol-evoked fluid secretion., Conclusions: A new method was introduced to investigate LG duct function. Water permeability of rabbit LG duct epithelium was measured by calculating filtration permeability. Fluid secretion of LG duct cells induced by carbachol or forskolin was also demonstrated. These results provide calculated values of lacrimal duct osmotic permeability and direct experimental evidence of LG duct fluid secretion., (Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.)
- Published
- 2014
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46. Indeterminate cell histiocytosis in a pediatric patient: successful treatment with thalidomide.
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Tóth B, Katona M, Hársing J, Szepesi A, and Kárpáti S
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- Adolescent, Histiocytosis pathology, Humans, Male, Skin Diseases pathology, Treatment Outcome, Histiocytosis drug therapy, Immunosuppressive Agents therapeutic use, Skin Diseases drug therapy, Thalidomide therapeutic use
- Abstract
The 15-year-old male patient presented several 2-6 mm large livid reddish-yellowish, shiny, compact papules on the head, trunk and extremities, which had developed within the last 4 months. Histology showed normal epidermis with dense dermal infiltrate of histiocytes accompanied by few eosinophils, Touton or foamy giant cells. The histiocytes were S100 positive, CD1a negative and did not contain Birbeck granules ultrastructurally. Chest X ray, EEG, skull MRI did not show pathology. Opthalmology, neurology, oto-rhino-laryngology did not reveal alterations. Based upon the clinical symptoms and the histopathology, the diagnosis of indeterminate cell histiocytosis was confirmed. Cryotherapy and cauterization did not stop the progression of the disease, however, under thalidomide treatment no new symptoms developed and the lesions healed with pigmentation.
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- 2012
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47. Unusual clinical history of a male infant with Edwards syndrome.
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Surányi A, Bitó T, Vajda G, Kaiser L, Gáspár G, Katona M, Szabó J, and Pál A
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- Abnormalities, Multiple genetics, Adult, Fatal Outcome, Female, Humans, Infant, Male, Syndrome, Abnormalities, Multiple diagnosis, Chromosome Aberrations, Chromosomes, Human, Pair 18 genetics, Trisomy
- Abstract
Edwards syndrome (trisomy of chromosome 18) is generally characterized by the disorders of central nervous system, as well as the musculoskeletal and genitourinary systems. In majority of the cases with trisomy 18 the following malformations can be found: ventricular septal defect, horseshoe kidneys, oesophageal atresia, omphalocele, facial clefts, diaphragmatic hernias and genital hypoplasia. We report a male patient with Edwards syndrome. The boy had a partial agenesis of corpus callosum, oesophageal atresia with tracheo-oesophageal fistula, renal agenesis, ventricular septal defect, Dandy-Walker cyst and low-set malformed ears. The first three features are unique based on previous literature reports on trisomy 18. This report allows a further delineation of the trisomy 18 syndrome.
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- 2009
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48. Selective detection of specific protein-ligand complexes by electrosonic spray-precursor ion scan tandem mass spectrometry.
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Czuczy N, Katona M, and Takats Z
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- Spectrometry, Mass, Electrospray Ionization methods, Trisaccharides chemistry, Cytochromes c chemistry, Ligands, Muramidase chemistry, Protein Binding, Ribonuclease, Pancreatic chemistry, Tandem Mass Spectrometry methods
- Abstract
A novel mass spectrometric method for the selective detection of specific protein-ligand complexes is presented. The new method is based on electrosonic spray ionization of samples containing protein and ligand molecules, and mass spectrometric detection using the precursor ion scanning function on a triple quadrupole instrument. Mass-selected intact protein-ligand complex ions are subjected to fragmentation by means of collision-induced dissociation in the collision cell of the instrument, while the second mass analyzer is set to the m/z of protonated ligand ions or their alkali metal adducts. The method allows for the detection of only those ions which yield ions characteristic of the ligand molecules upon fragmentation. Since the scan range of first analyzer is set well above the m/z of the ligand ion, and the CID conditions are established to permit fragmentation of only loosely bound, noncovalent complexes, the method is specific to the detection of protein-ligand complexes under described conditions. Behavior of biologically specific and nonspecific complexes was compared under various instrumental settings. Parameters were optimized to obtain maximal selectivity for specific complexes. Specific and nonspecific complexes were found to show markedly different fragmentation characteristics, which can be a basis for selective detection of complexes with biological relevance. Preparation of specific and nonspecific complexes containing identical building blocks was attempted. Complex ions with identical stoichiometry but different origin showed the expected difference in fragmentation characteristics, which gives direct evidence for the different mechanism of specific versus nonspecific complex ion formation.
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- 2009
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49. Easy method for keratin 14 gene amplification to exclude pseudogene sequences: new keratin 5 and 14 mutations in epidermolysis bullosa simplex.
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Glász-Bóna A, Medvecz M, Sajó R, Lepesi-Benko R, Tulassay Z, Katona M, Hatvani Z, Blazsek A, and Kárpáti S
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- Alleles, Codon, DNA Mutational Analysis, Exons, Family Health, Gene Amplification, Genes, Dominant, Genetic Techniques, Humans, Keratin-14 metabolism, Keratin-5 metabolism, Pseudogenes, Dermatology methods, Keratin-14 genetics, Keratin-5 genetics, Mutation
- Published
- 2009
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50. Medical school applications. Pool of potential medical students needs to be enlarged.
- Author
-
Katona C, Katona P, and Katona M
- Subjects
- Educational Status, Humans, United Kingdom, School Admission Criteria, Schools, Medical
- Published
- 2003
- Full Text
- View/download PDF
Catalog
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