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6. Matricellular proteins in cutaneous wound healing

Author

Cardenas-Leon, Claudia Griselda, Mäemets-Allas, Kristina, Klaas, Mariliis, Lagus, Heli, Kankuri, Esko, Jaks, Viljar, HUS Musculoskeletal and Plastic Surgery, Clinicum, University of Helsinki, Plastiikkakirurgian yksikkö, Research Programs Unit, Medicum, Department of Pharmacology, and Esko Markus Kankuri / Principal Investigator

Subjects
Regeneration, 1182 Biochemistry, cell and molecular biology, Extracellular matrix, Non-structural matrix proteins, Skin, Re-epithelialization
Abstract

Cutaneous wound healing is a complex process that encompasses alterations in all aspects of the skin including the extracellular matrix (ECM). ECM consist of large structural proteins such as collagens and elastin as well as smaller proteins with mainly regulative properties called matricellular proteins. Matricellular proteins bind to structural proteins and their functions include but are not limited to interaction with cell surface receptors, cytokines, or protease and evoking a cellular response. The signaling initiated by matricellular proteins modulates differentiation and proliferation of cells having an impact on the tissue regeneration. In this review we give an overview of the matricellular proteins that have been found to be involved in cutaneous wound healing and summarize the information known to date about their functions in this process.

Published
2022

8. Thrombospondin-4 Is a Soluble Dermal Inflammatory Signal That Selectively Promotes Fibroblast Migration and Keratinocyte Proliferation for Skin Regeneration and Wound Healing

Author

Klaas, Mariliis, primary, Mäemets-Allas, Kristina, additional, Heinmäe, Elizabeth, additional, Lagus, Heli, additional, Cárdenas-León, Claudia Griselda, additional, Arak, Terje, additional, Eller, Mart, additional, Kingo, Külli, additional, Kankuri, Esko, additional, and Jaks, Viljar, additional

Published
2021
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12. Endogenous beta‐galactosidase activity marks a TREM2‐expressing Kupffer cell population in injured livers of Lgr5‐LacZ and wild‐type mice.

Author

Klaas, Mariliis, Mäemets‐Allas, Kristina, Lõhmussaar, Kadi, Tooming, Mikk, Viil, Janeli, and Jaks, Viljar

Subjects
*KUPFFER cells, *CELL populations, *LIVER cells, *LIVER, *GENE expression profiling, *PROGENITOR cells, *TRANSGENE expression
Abstract

Lgr5‐LacZ mice harbor the Escherichia coli LacZ gene encoding β‐galactosidase (β‐gal) under the control of the Lgr5 promoter, a stem/progenitor cell marker. In injured livers of Lgr5‐LacZ mice, cells expressing β‐galactosidase (β‐gal) are considered as potential bipotent liver progenitors; however, their origin and identity remain unknown. Unexpectedly, using lineage tracing, we demonstrate that the β‐gal+ cells do not originate from liver parenchymal cells. Instead, β‐gal+ cells, isolated from injured livers of both Lgr5‐LacZ and wild‐type mice, are positive for markers of Kupffer cells, liver‐resident macrophages. The β‐gal expression in these cells is a result of elevated expression of the endogenous beta‐galactosidase Glb1. In injured livers of Lgr5‐LacZ mice, bacterial β‐gal expression is very low, suggesting transgene silencing. The gene expression profile of the β‐gal+ Kupffer cells from injured livers suggests a role in liver regeneration. [ABSTRACT FROM AUTHOR]

Published
2020
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14. Pre-administration of PepFect6-microRNA-146a nanocomplexes inhibits inflammatory responses in keratinocytes and in a mouse model of irritant contact dermatitis

Author

Urgard, Egon, Lorents, Annely, Klaas, Mariliis, Padari, Kart, Viil, Janeli, Runnel, Toomas, Langel, Kent, Kingo, Kulli, Tkaczyk, Eric, Langel, Ülo, Maimets, Toivo, Jaks, Viljar, Pooga, Margus, Rebane, Ana, Urgard, Egon, Lorents, Annely, Klaas, Mariliis, Padari, Kart, Viil, Janeli, Runnel, Toomas, Langel, Kent, Kingo, Kulli, Tkaczyk, Eric, Langel, Ülo, Maimets, Toivo, Jaks, Viljar, Pooga, Margus, and Rebane, Ana

Abstract

The skin is a difficult to access tissue for efficient delivery of large and/or chargedmacromolecules, including therapeutic DNA and RNA oligonucleotides. Cell-penetrating peptide PepFect6 (PF6) has been shown to be suitable transport vehicle for siRNAs in cell culture and systemically in vivo in mice. MiR-146a is known as anti-inflammatory miRNA that inhibits multiple factors fromthe nuclear factor (NF)-kappa B pathway in various cell types, including keratinocytes. In this study, PF6 was shown to form unimodal nanocomplexes with miR-146a mimic that entered into human primary keratinocytes, where miR-146a inhibited the expression of its direct targets fromthe NF-kappa B pathway and the genes known to be activated by NF-kappa B, C-C motif ligand (CCL)5 and interleukin (IL)-8. The transfection of miR-146a mimic with PF6 was more efficient in sub-confluent keratinocyte cultures, affected keratinocyte proliferation less and had similar effect on cell viability when compared with a lipid based agent. Subcutaneous pre-administration of PF6-miR-146a nanocomplexes attenuated ear-swelling and reduced the expression of pro-inflammatory cytokines and chemokines IL-6, CCL11, CCL24 and C-X-C motif ligand 1 (CXCL1) in a mouse model of irritant contact dermatitis. Our data demonstrates that PF6-miR-146a nanoparticles might have potential in the development of therapeutics to target inflammatory skin diseases.

Published
2016
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16. miR-146a/b mõju interleukiin-1 perekonna geeniekspressioonile bronhiepiteeli rakkudes rinoviirusinfektsiooni korral

Author

Mäe, Mariel, Kivihall, Anett, juhendaja, Klaas, Mariliis, juhendaja, Tartu Ülikool. Loodus- ja täppisteaduste valdkond, and Tartu Ülikool. Molekulaar- ja rakubioloogia instituut

Subjects
rinoviirus, interleukiin, bakalaureusetööd, astma, miR-146, põletik
Abstract

Astma on krooniline hingamisteede haigus, mille ägenemist põhjustab sageli rinoviirusinfektsioon. Samuti on haiguse korral täheldatud muutunud interleukiin-1 (IL-1) perekonna tsütokiinide ja geeniekspressiooni regulaatori miR-146 perekonna liikmete miR-146a ja miR-146b (miR-146a/b) ekspressiooni. Nende kolme faktori omavahelisi seoseid seoses astma ja astma ägenemisega ei ole veel uuritud, mistõttu oli antud bakalaureusetöö eesmärgiks tuvastada rinoviirusinfektsiooni mõju IL-1 perekonna geenide ekspressioonile ning analüüsida miR-146a/b rolli IL-1 perekonna geenide ekspressiooni reguleerimisel rinoviirusinfektsiooni korral. Käesoleva bakalaureusetöö tulemusena selgus, et rinoviirusinfektsioon põhjustab bronhiepiteeli rakkudes suurenenud IL-1 perekonna geenide ekspressiooni ning miR-146a/b käituvad rinoviirusnakkuse korral IL-1 perekonna geenide negatiivse regulaatorina

Published
2020

18. Prolüülendopeptidaasi roll neuronaalsete rakkude adhesioonimolekulide häirunud ekspressioonis ja selle analüüsi metoodikate hindamine neuroblastoomi SH-SY5Y rakuliinis

Author

Pajo, Kadri, Klaas,Mariliis, juhendaja, Jaako, Külli, juhendaja, Tartu Ülikool. Loodus- ja tehnoloogiateaduskond, and Tartu Ülikool. Molekulaar- ja rakubioloogia instituut

Subjects
ajuplastilisus, Neutraalne raku adhesioonimolekul, prolüülendopeptidaas, bakalaureusetööd, maatriksi metalloproteinaas-9, polüsialüleeritud neutraalne raku adhesioonimolekul
Published
2015

19. Meditsiinilise titaani funktsionaliseerimine aatomkihtsadestatud kiledega

Author

Aaviksoo, Elyne, Merisalu, Maido, juhendaja, Klaas, Mariliis, juhendaja, Sammelselg, Väino, juhendaja, Tartu Ülikool. Loodus- ja tehnoloogiateaduskond, and Tartu Ülikool. Füüsika instituut

Subjects
bakalaureusetööd, implantaadid, aatomkihtsadestamine, titaan, õhukesed kiled
Abstract

http://www.ester.ee/record=b4616792*est

Published
2015

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