47 results on '"Köhli, M."'
Search Results
2. Three years of soil moisture observations by a dense cosmic-ray neutron sensing cluster at an agricultural research site in north-east Germany [Data set]
- Author
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Heistermann, M., Francke, T., Scheiffele, L., Dimitrova Petrova, K., Budach, C., Schrön, Martin, Trost, B., Rasche, D., Güntner, A., Döpper, V., Förster, M., Köhli, M., Antonoglou, N., Zude-Sasse, M., Oswald, S.E., Heistermann, M., Francke, T., Scheiffele, L., Dimitrova Petrova, K., Budach, C., Schrön, Martin, Trost, B., Rasche, D., Güntner, A., Döpper, V., Förster, M., Köhli, M., Antonoglou, N., Zude-Sasse, M., and Oswald, S.E.
- Abstract
Cosmic-ray neutron sensing (CRNS) allows for the estimation of root-zone soil water content (SWC) at the scale of several hectares. In this paper, we present the data recorded by a dense CRNS network operated from 2019 to 2022 at an agricultural research site in Marquardt, Germany – the first multi-year CRNS cluster. Consisting, at its core, of eight permanently installed CRNS sensors, the cluster was supplemented by a wealth of complementary measurements: data from seven additional temporary CRNS sensors, partly co-located with the permanent ones; 27 SWC profiles (mostly permanent); two groundwater observation wells; meteorological records; and Global Navigation Satellite System reflectometry (GNSS-R). Complementary to these continuous measurements, numerous campaign-based activities provided data by mobile CRNS roving, hyperspectral imagery via UASs, intensive manual sampling of soil properties (SWC, bulk density, organic matter, texture, soil hydraulic properties), and observations of biomass and snow (cover, depth, and density). The unique temporal coverage of 3 years entails a broad spectrum of hydro-meteorological conditions, including exceptional drought periods and extreme rainfall but also episodes of snow coverage, as well as a dedicated irrigation experiment. Apart from serving to advance CRNS-related retrieval methods, this data set is expected to be useful for various disciplines, for example, soil and groundwater hydrology, agriculture, or remote sensing. Hence, we show exemplary features of the data set in order to highlight the potential for such subsequent studies.
- Published
- 2023
3. Comment on ‘Examining the variation of soil moisture from cosmic-ray neutron probes footprint: experimental results from a COSMOS-UK site’ by Howells, O.D., Petropoulos, G.P., et al., Environ Earth Sci 82, 41 (2023)
- Author
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Scheiffele, L.M., Schrön, Martin, Köhli, M., Dimitrova-Petrova, K., Altdorff, Daniel, Franz, T., Rosolem, R., Evans, J., Blake, J., Bogena, H., McJannet, D., Baroni, G., Desilets, D., Oswald, S.E., Scheiffele, L.M., Schrön, Martin, Köhli, M., Dimitrova-Petrova, K., Altdorff, Daniel, Franz, T., Rosolem, R., Evans, J., Blake, J., Bogena, H., McJannet, D., Baroni, G., Desilets, D., and Oswald, S.E.
- Abstract
no abstract
- Published
- 2023
4. URANOS v1.0 – the Ultra Rapid Adaptable Neutron-Only Simulation for environmental research
- Author
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Köhli, M., Schrön, Martin, Zacharias, Steffen, Schmidt, U., Köhli, M., Schrön, Martin, Zacharias, Steffen, and Schmidt, U.
- Abstract
The understanding of neutron transport by Monte Carlo simulations led to major advancements towards precise interpretation of measurements. URANOS (Ultra Rapid Neutron-Only Simulation) is a free software package which has been developed in the last few years in cooperation with particle physics and environmental sciences, specifically for the purposes of cosmic-ray neutron sensing (CRNS). Its versatile user interface and input/output scheme tailored for CRNS applications offers hydrologists straightforward access to model individual scenarios and to directly perform advanced neutron transport calculations. The geometry can be modeled layer-wise, whereas in each layer a voxel geometry is extruded using a two-dimensional map from pixel images representing predefined materials and allowing for the construction of objects on the basis of pixel graphics without a three-dimensional editor. It furthermore features predefined cosmic-ray neutron spectra and detector configurations and also allows for a replication of important site characteristics of study areas – from a small pond to the catchment scale. The simulation thereby gives precise answers to questions like from which location do neutrons originate? How do they propagate to the sensor? What is the neutron's response to certain environmental changes? In recent years, URANOS has been successfully employed by a number of studies, for example, to calculate the cosmic-ray neutron footprint, signals in complex geometries like mobile applications on roads, urban environments and snow patterns.
- Published
- 2023
5. Signal contribution of distant areas to cosmic-ray neutron sensors – implications for footprint and sensitivity
- Author
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Schrön, Martin, Köhli, M., Zacharias, Steffen, Schrön, Martin, Köhli, M., and Zacharias, Steffen
- Abstract
This paper presents a new theoretical approach to estimate the contribution of distant areas to the measurement signal of cosmic-ray neutron detectors for snow and soil moisture monitoring. The algorithm is based on the local neutron production and the transport mechanism, given by the neutron–moisture relationship and the radial intensity function, respectively. The purely analytical approach has been validated with physics-based neutron transport simulations for heterogeneous soil moisture patterns, exemplary landscape features, and remote fields at a distance. We found that the method provides good approximations of simulated signal contributions in patchy soils with typical deviations of less than 1 %. Moreover, implications of this concept have been investigated for the neutron–moisture relationship, where the signal contribution of an area has the potential to explain deviating shapes of this curve that are often reported in the literature. Finally, the method has been used to develop a new practical footprint definition to express whether or not a distant area's soil moisture change is actually detectable in terms of measurement precision. The presented concepts answer long-lasting questions about the influence of distant landscape structures in the integral footprint of the sensor without the need for computationally expensive simulations. The new insights are highly relevant to support signal interpretation, data harmonization, and sensor calibration and will be particularly useful for sensors positioned in complex terrain or on agriculturally managed sites.
- Published
- 2023
6. Three years of soil moisture observations by a dense cosmic-ray neutron sensing cluster at an agricultural research site in north-east Germany
- Author
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Heistermann, M., Francke, T., Scheiffele, L., Dimitrova Petrova, K., Budach, C., Schrön, Martin, Trost, B., Rasche, D., Güntner, A., Döpper, V., Förster, M., Köhli, M., Angermann, L., Antonoglou, N., Zude-Sasse, M., Oswald, S.E., Heistermann, M., Francke, T., Scheiffele, L., Dimitrova Petrova, K., Budach, C., Schrön, Martin, Trost, B., Rasche, D., Güntner, A., Döpper, V., Förster, M., Köhli, M., Angermann, L., Antonoglou, N., Zude-Sasse, M., and Oswald, S.E.
- Abstract
Cosmic-ray neutron sensing (CRNS) allows for the estimation of root-zone soil water content (SWC) at the scale of several hectares. In this paper, we present the data recorded by a dense CRNS network operated from 2019 to 2022 at an agricultural research site in Marquardt, Germany – the first multi-year CRNS cluster. Consisting, at its core, of eight permanently installed CRNS sensors, the cluster was supplemented by a wealth of complementary measurements: data from seven additional temporary CRNS sensors, partly co-located with the permanent ones; 27 SWC profiles (mostly permanent); two groundwater observation wells; meteorological records; and Global Navigation Satellite System reflectometry (GNSS-R). Complementary to these continuous measurements, numerous campaign-based activities provided data by mobile CRNS roving, hyperspectral imagery via UASs, intensive manual sampling of soil properties (SWC, bulk density, organic matter, texture, soil hydraulic properties), and observations of biomass and snow (cover, depth, and density). The unique temporal coverage of 3 years entails a broad spectrum of hydro-meteorological conditions, including exceptional drought periods and extreme rainfall but also episodes of snow coverage, as well as a dedicated irrigation experiment. Apart from serving to advance CRNS-related retrieval methods, this data set is expected to be useful for various disciplines, for example, soil and groundwater hydrology, agriculture, or remote sensing. Hence, we show exemplary features of the data set in order to highlight the potential for such subsequent studies.
- Published
- 2023
7. A change in perspective: downhole cosmic-ray neutron sensing for the estimation of soil moisture
- Author
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Rasche, D., Weimar, J., Schrön, Martin, Köhli, M., Morgner, M., Güntner, A., Blume, T., Rasche, D., Weimar, J., Schrön, Martin, Köhli, M., Morgner, M., Güntner, A., and Blume, T.
- Abstract
Above-ground cosmic-ray neutron sensing (CRNS) allows for the non-invasive estimation of the field-scale soil moisture content in the upper decimetres of the soil. However, large parts of the deeper vadose zone remain outside of its observational window. Retrieving soil moisture information from these deeper layers requires extrapolation, modelling or other methods, all of which come with methodological challenges. Against this background, we investigate CRNS for downhole soil moisture measurements in deeper layers of the vadose zone. To render calibration with in situ soil moisture measurements unnecessary, we rescaled neutron intensities observed below the terrain surface with intensities measured above a waterbody. An experimental set-up with a CRNS sensor deployed at different depths of up to 10 m below the surface in a groundwater observation well combined with particle transport simulations revealed the response of downhole thermal neutron intensities to changes in the soil moisture content at the depth of the downhole neutron detector as well as in the layers above it. The simulation results suggest that the sensitive measurement radius of several decimetres, which depends on soil moisture and soil bulk density, exceeds that of a standard active neutron probe (which is only about 30 cm). We derived transfer functions to estimate downhole neutron signals from soil moisture information, and we describe approaches for using these transfer functions in an inverse way to derive soil moisture from the observed neutron signals. The in situ neutron and soil moisture observations confirm the applicability of these functions and prove the concept of passive downhole soil moisture estimation, even at larger depths, using cosmic-ray neutron sensing.
- Published
- 2023
8. Assessing the feasibility of a directional cosmic-ray neutron sensing sensor for estimating soil moisture
- Author
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Francke, T., Heistermann, M., Köhli, M., Budach, C., Schrön, Martin, Oswald, S.E., Francke, T., Heistermann, M., Köhli, M., Budach, C., Schrön, Martin, and Oswald, S.E.
- Abstract
Cosmic-ray neutron sensing (CRNS) is a non-invasive tool for measuring hydrogen pools such as soil moisture, snow or vegetation. The intrinsic integration over a radial hectare-scale footprint is a clear advantage for averaging out small-scale heterogeneity, but on the other hand the data may become hard to interpret in complex terrain with patchy land use. This study presents a directional shielding approach to prevent neutrons from certain angles from being counted while counting neutrons entering the detector from other angles and explores its potential to gain a sharper horizontal view on the surrounding soil moisture distribution. Using the Monte Carlo code URANOS (Ultra Rapid Neutron-Only Simulation), we modelled the effect of additional polyethylene shields on the horizontal field of view and assessed its impact on the epithermal count rate, propagated uncertainties and aggregation time. The results demonstrate that directional CRNS measurements are strongly dominated by isotropic neutron transport, which dilutes the signal of the targeted direction especially from the far field. For typical count rates of customary CRNS stations, directional shielding of half-spaces could not lead to acceptable precision at a daily time resolution. However, the mere statistical distinction of two rates should be feasible.
- Published
- 2022
9. The Footprint Characteristics of Cosmic Ray Thermal Neutrons
- Author
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Jakobi, J., primary, Huisman, J. A., additional, Köhli, M., additional, Rasche, D., additional, Vereecken, H., additional, and Bogena, H. R., additional
- Published
- 2021
- Full Text
- View/download PDF
10. EE594 Comparison of the Clinical and Economic Impact of Two COVID-19 Vaccines in Immunocompromised Patients in France
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Uhart, M., Cheret, A., Van de Velde, N., Lee, A., and Kohli, M.
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- 2023
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11. EE287 Epidemiologic and Economic Impact of COVID-19 Vaccination Among at-Risk Individuals and Individuals Aged ≥60 Years in Germany
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Joshi, K., Kohli, M., Maschio, M., Lee, A., Fust, K., Beck, E., Van de Velde, N., Scholz, S., and Ultsch, B.
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- 2023
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12. EE140 Comparison of the Clinical and Economic Impact of Two MRNA COVID-19 Vaccines in High Risk Individuals in Japan
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Kohli, M., Hagiwara, Y., Igarashi, A., Joshi, K., Lee, A., Maschio, M., Van de Velde, N., and Beck, E.
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- 2023
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13. Soil moisture and air humidity dependence of the above-ground cosmic-ray neutron intensity
- Author
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Köhli, M., Weimar, J., Schrön, Martin, Baatz, R., Schmidt, U., Köhli, M., Weimar, J., Schrön, Martin, Baatz, R., and Schmidt, U.
- Abstract
Investigations of neutron transport through air and soil by Monte Carlo simulations led to major advancements towards a precise interpretation of measurements, especially they improved the understanding of the cosmic-ray neutron footprint. Up to now, the conversion of soil moisture to a detectable neutron count rate relies mainly on the equation presented by Desilets et al. (2010). While in general a hyperbolic expression can be derived from theoretical considerations, their empiric parameterisation needs to be revised for two reasons. Firstly, a rigorous mathematical treatment reveals that the values of the four parameters are ambiguous because their values are not independent. We find a 3-parameter equation with unambiguous values of the parameters which is equivalent in any other respect to the 4-parameter equation. Secondly, high-resolution Monte-Carlo simulations revealed a systematic deviation of the count rate to soil moisture relation especially for extremely dry conditions as well as very humid conditions. That is a hint, that a smaller contribution to the intensity was forgotten or not adequately treated by the conventional approach. Investigating the above-ground neutron flux by a broadly based Monte-Carlo simulation campaign revealed a more detailed understanding of different contributions to this signal, especially targeting air humidity corrections. The packages MCNP and URANOS were used to derive a function able to describe the respective dependencies including the effect of different hydrogen pools and the detector-specific response function. The new relationship has been tested at two exemplary measurement sites and its remarkable performance allows for a promising prospect of more comprehensive data quality in the future.
- Published
- 2021
14. Towards disentangling heterogeneous soil moisture patterns in cosmic-ray neutron sensor footprints
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Rasche, D., Köhli, M., Schrön, Martin, Blume, T., Güntner, A., Rasche, D., Köhli, M., Schrön, Martin, Blume, T., and Güntner, A.
- Abstract
Cosmic-ray neutron sensing (CRNS) allows for non-invasive soil moisture estimations at the field scale. The derivation of soil moisture generally relies on secondary cosmic-ray neutrons in the epithermal to fast energy ranges. Most approaches and processing techniques for observed neutron intensities are based on the assumption of homogeneous site conditions or of soil moisture patterns with correlation lengths shorter than the measurement footprint of the neutron detector. However, in view of the non-linear relationship between neutron intensities and soil moisture, it is questionable whether these assumptions are applicable. In this study, we investigated how a non-uniform soil moisture distribution within the footprint impacts the CRNS soil moisture estimation and how the combined use of epithermal and thermal neutrons can be advantageous in this case. Thermal neutrons have lower energies and a substantially smaller measurement footprint around the sensor than epithermal neutrons. Analyses using the URANOS (Ultra RApid Neutron-Only Simulation) Monte Carlo simulations to investigate the measurement footprint dynamics at a study site in northeastern Germany revealed that the thermal footprint mainly covers mineral soils in the near-field to the sensor while the epithermal footprint also covers large areas with organic soils. We found that either combining the observed thermal and epithermal neutron intensities by a rescaling method developed in this study or adjusting all parameters of the transfer function leads to an improved calibration against the reference soil moisture measurements in the near-field compared to the standard approach and using epithermal neutrons alone. We also found that the relationship between thermal and epithermal neutrons provided an indicator for footprint heterogeneity. We, therefore, suggest that the combined use of thermal and epithermal neutrons offers the potential of a spatial disaggregation of the measurement footprint in terms o
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- 2021
15. EE329 The Value-Based Price for a Bivalent COVID-19 Booster Vaccine in the United Kingdom
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Kohli, M., Maschio, M., Lee, A., Carroll, S., Van de Velde, N., and Drummond, M.
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- 2023
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16. EE338 The Potential Benefit of Respiratory Syncytial Virus (RSV) Vaccination in Older Adults: A Comparison of the United States (US) and United Kingdom (UK)
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Fust, K, Kohli, M, Panozzo, CA, Weinstein, MC, Buck, P, and Gaswalla, P
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- 2022
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17. EE80 The Potential Clinical and Economic Benefits of a Next Generation Omicron-Containing (BA.1) Bivalent Sars-Cov-2 Booster Compared to First Generation Prototype Boosters in Germany Assuming BA.4/BA.5 Dominance
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Maschio, M, Schweikert, B, Ultsch, B, Cai, R, Fust, K, Lee, A, Nasir, A, Buck, P, Van de Velde, N, and Kohli, M
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- 2022
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18. A dense network of cosmic-ray neutron sensors for soil moisture observation in a highly instrumented pre-Alpine headwater catchment in Germany
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Fersch, B., Francke, T., Heistermann, M., Schrön, Martin, Döpper, V., Jakobi, J., Baroni, G., Blume, T., Bogena, H., Budach, C., Gränzig, T., Förster, M., Güntner, A., Hendricks Franssen, H.-J., Kasner, Mandy, Köhli, M., Kleinschmit, B., Kunstmann, H., Patil, A., Rasche, D., Scheiffele, L., Schmidt, U., Szulc-Seyfried, S., Weimar, J., Zacharias, Steffen, Zreda, M., Heber, B., Kiese, R., Mares, V., Mollenhauer, Hannes, Völksch, I., Oswald, S., Fersch, B., Francke, T., Heistermann, M., Schrön, Martin, Döpper, V., Jakobi, J., Baroni, G., Blume, T., Bogena, H., Budach, C., Gränzig, T., Förster, M., Güntner, A., Hendricks Franssen, H.-J., Kasner, Mandy, Köhli, M., Kleinschmit, B., Kunstmann, H., Patil, A., Rasche, D., Scheiffele, L., Schmidt, U., Szulc-Seyfried, S., Weimar, J., Zacharias, Steffen, Zreda, M., Heber, B., Kiese, R., Mares, V., Mollenhauer, Hannes, Völksch, I., and Oswald, S.
- Abstract
Monitoring soil moisture is still a challenge: it varies strongly in space and time and at various scales while conventional sensors typically suffer from small spatial support. With a sensor footprint up to several hectares, cosmic-ray neutron sensing (CRNS) is a modern technology to address that challenge. So far, the CRNS method has typically been applied with single sensors or in sparse national-scale networks. This study presents, for the first time, a dense network of 24 CRNS stations that covered, from May to July 2019, an area of just 1 km(2): the pre-Alpine Rott headwater catchment in Southern Germany, which is characterized by strong soil moisture gradients in a heterogeneous landscape with forests and grasslands. With substantially overlapping sensor footprints, this network was designed to study root-zone soil moisture dynamics at the catchment scale. The observations of the dense CRNS network were complemented by extensive measurements that allow users to study soil moisture variability at various spatial scales: roving (mobile) CRNS units, remotely sensed thermal images from unmanned areal systems (UASs), permanent and temporary wireless sensor networks, profile probes, and comprehensive manual soil sampling. Since neutron counts are also affected by hydrogen pools other than soil moisture, vegetation biomass was monitored in forest and grassland patches, as well as meteorological variables; discharge and groundwater tables were recorded to support hydrological modeling experiments. As a result, we provide a unique and comprehensive data set to several research communities: to those who investigate the retrieval of soil moisture from cosmic-ray neutron sensing, to those who study the variability of soil moisture at different spatiotemporal scales, and to those who intend to better understand the role of rootzone soil moisture dynamics in the context of catchment and groundwater hydrology, as well as land-atmosphere exchange processes. The data set is a
- Published
- 2020
19. EE479 Cost-Effectiveness of Olaparib Compared to Rucaparib for the Treatment of Metastatic Castration-Resistant Prostate Cancer (MCRPC) in United States
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Ghule, P, Kohli, M, Malone, DC, and Nelson, RE
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- 2022
- Full Text
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20. Sensing area‐average snow water equivalent with cosmic‐ray neutrons: The influence of fractional snow cover
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Schattan, P., Köhli, M., Schrön, Martin, Baroni, G., Oswald, S.E., Schattan, P., Köhli, M., Schrön, Martin, Baroni, G., and Oswald, S.E.
- Abstract
Cosmic‐ray neutron sensing (CRNS) is a promising non‐invasive technique to estimate snow water equivalent (SWE) over large areas. In contrast to preliminary studies focusing on shallow snow conditions (SWE 130 mm), more recently the method was shown experimentally to be sensitive also to deeper snowpacks providing the basis for its use at mountain experimental sites. However, hysteretic neutron response has been observed for complex snow cover including patchy snow‐free areas. In the present study we aimed to understand and support the experimental findings using a comprehensive neutron modeling approach. Several simulations have been set up in order to disentangle the effect on the signal of different land surface characteristics and to reproduce multiple observations during periods of snow melt and accumulation. To represent the actual land surface heterogeneity and the complex snow cover, the model used data from terrestrial laser scanning. The results show that the model was able to accurately reproduce the CRNS signal and particularly the hysteresis effect during accumulation and melting periods. Moreover, the sensor footprint was found to be anisotropic and affected by the spatial distribution of liquid water and snow as well as by the topography of the nearby mountains. Under fully snow‐covered conditions the CRNS is able to accurately estimate SWE without prior knowledge about snow density profiles or other spatial anomalies. These results provide new insights into the characteristics of the detected neutron signal in complex terrain and support the use of CRNS for long‐term snow monitoring in high elevated mountain environments.
- Published
- 2019
21. Can drip irrigation be scheduled with cosmic-ray neutron sensing?
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Li, D., Schrön, Martin, Köhli, M., Bogena, H., Weimar, Jannis, Jiménez Bello, M.A., Han, X., Martínez Gimeno, M.A., Zacharias, Steffen, Vereecken, H., Hendricks Franssen, H.-J., Li, D., Schrön, Martin, Köhli, M., Bogena, H., Weimar, Jannis, Jiménez Bello, M.A., Han, X., Martínez Gimeno, M.A., Zacharias, Steffen, Vereecken, H., and Hendricks Franssen, H.-J.
- Abstract
Irrigation is essential for maintaining food production in water-scarce regions. The irrigation need depends on the water content of the soil, which we measured with the novel technique of cosmic-ray neutron sensing (CRNS). The potential of the CRNS technique for drip irrigation scheduling was explored in this study for the Picassent site near Valencia, Spain. To support the experimental evidence, the neutron transport simulation URANOS was used to simulate the effect of drip irrigation on the neutron counts. The overall soil water content (SWC) in the CRNS footprint was characterized with a root mean square error <0.03 cm3/cm3, but the experimental dataset indicated methodological limitations to detect drip water input. Both experimental data and simulation results suggest that the large-area neutron response to drip irrigation is insignificant in our specific case using a standard CRNS probe. Because of the small area of irrigated patches and short irrigation time, the limited SWC changes due to drip irrigation were not visible from the measured neutron intensity changes. Our study shows that CRNS modeling can be used to assess the suitability of the CRNS technique for certain applications. While the standard CRNS probe was not able to detect small-scale drip irrigation patterns, the method might be applicable for larger irrigated areas, in drier regions, and for longer and more intense irrigation periods. Since statistical noise is the main limitation of the CRNS measurement, the capability of the instrument could be improved in future studies by larger and more efficient neutron detectors.
- Published
- 2019
22. 596P Impact of combined lipidomic and genetic aberrations on clinical outcomes in metastatic castration-resistant prostate cancer (mCRPC)
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Mak, B., Lin, H-M., Kwan, E.M., Fettke, H., Tran, B., Davis, I.D., Mahon, K.L., Stockler, M.R., Briscoe, K., Marx, G.M., Kohli, M., Tan, W., Huynh, K., Du, P., Yu, J., Jia, S., Joshua, A.M., Azad, A.A., Meikle, P.J., and Horvath, L.G.
- Published
- 2021
- Full Text
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23. Response functions for detectors in cosmic ray neutron sensing
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Köhli, M., Schrön, Martin, Schmidt, U., Köhli, M., Schrön, Martin, and Schmidt, U.
- Abstract
Cosmic-Ray Neutron Sensing (CRNS) is a novel technique for determining environmental water content by measuring albedo neutrons in the epithermal to fast energy range with moderated neutron detectors. We have investigated the response function of stationary and mobile neutron detectors typically used for environmental research in order to improve the model accuracy for neutron transport studies. Monte Carlo simulations have been performed in order to analyze the detection probability in terms of energy-dependent response and angular sensitivity for different variants of CRNS detectors and converter gases. Our results reveal the sensor’s response to neutron energies from 0.1 eV to 106 eV and highest sensitivity to vertical fluxes. The detector efficiency shows good agreement with reference data from the structurally similar Bonner Spheres. The relative probability of neutrons contributing to the overall integrated signal is especially important in regions with non-uniform albedo fluxes, such as complex terrain or heterogeneous distribution of hydrogen pools
- Published
- 2018
24. Monitoring environmental water with ground albedo neutrons and correction for incoming cosmic rays with neutron monitor data
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Schrön, Martin, Zacharias, Steffen, Köhli, M., Weimar, Jannis, Dietrich, Peter ; orcid:0000-0003-2699-2354, Schrön, Martin, Zacharias, Steffen, Köhli, M., Weimar, Jannis, and Dietrich, Peter ; orcid:0000-0003-2699-2354
- Abstract
Neutron monitors on the Earth’s surface are usually used to track the dynamics of incoming cosmic-ray neutrons of high energy under the assumption that local environmental conditions do not influence the highly shielded flux. Oppositely, in a recently established research field the local dynamics of environmental water is monitored by detecting low-energy cosmic-ray neutrons. Water in soil, air, snow and vegetation determines the amount of ground albedo neutrons in the sensitive energy range from 1 eV to 100 keV. Plenty of small neutron detectors have been installed on natural or agricultural sites all around the world Climate research, hydrologic models and irrigation management rely on these measurements, which represent area-average water content within tens of hectares due to the fast diffusion of neutrons in air. A major issue is the modulation of the neutron flux by the dynamics of incoming cosmic-ray neutrons. Conventionally,independent data from neutron monitors are consulted to serve as a reference for the correction of the local detectors. However, the performance of this comparative correction approach is unreliable, because it does not account for geographical displacement, different energy windows of the detectors, or potential influence of atmospheric conditions on the referenced neutron monitor. In addition, neutron monitor stations are sparse on Earth, and occasionally signals from different locations appear to be significantly inconsistent. The presentation shows how ground albedo neutrons from cosmic-rays are used in environmental research and emphasizes the need for a reliable correction for the incoming flux.
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- 2016
25. CASCADE - a multi-layer Boron-10 neutron detection system
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Köhli, M, primary, Klein, M, additional, Allmendinger, F, additional, Perrevoort, A-K, additional, Schröder, T, additional, Martin, N, additional, Schmidt, C J, additional, and Schmidt, U, additional
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- 2016
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26. Footprint characteristics revised for field-scale soil moisture monitoring with cosmic-ray neutrons
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Köhli, M., Schrön, Martin, Zreda, M., Schmidt, U., Dietrich, Peter, Zacharias, Steffen, Köhli, M., Schrön, Martin, Zreda, M., Schmidt, U., Dietrich, Peter, and Zacharias, Steffen
- Abstract
Cosmic-ray neutron probes are widely used to monitor environmental water content near the surface. The method averages over tens of hectares and is unrivaled in serving representative data for agriculture and hydrological models at the hectometer scale. Recent experiments, however, indicate that the sensor response to environmental heterogeneity is not fully understood. Knowledge of the support volume is a prerequisite for the proper interpretation and validation of hydrogeophysical data. In a previous study, several physical simplifications have been introduced into a neutron transport model in order to derive the characteristics of the cosmic-ray probe's footprint. We utilize a refined source and energy spectrum for cosmic-ray neutrons and simulate their response to a variety of environmental conditions. Results indicate that the method is particularly sensitive to soil moisture in the first tens of meters around the probe, whereas the radial weights are changing dynamically with ambient water. The footprint radius ranges from 130 to 240 m depending on air humidity, soil moisture, and vegetation. The moisture-dependent penetration depth of 15 to 83 cm decreases exponentially with distance to the sensor. However, the footprint circle remains almost isotropic in complex terrain with nearby rivers, roads or hill slopes. Our findings suggest that a dynamically weighted average of point measurements is essential for accurate calibration and validation. The new insights will have important impact on signal interpretation, sensor installation, data interpolation from mobile surveys, and the choice of appropriate resolutions for data assimilation into hydrological models.
- Published
- 2015
27. PSY25 - A Budget Impact Model of The Addition of Telotristat Ethyl Treatment In Patients With Uncontrolled Carcinoid Syndrome
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Fust, K, Maschio, M, Pastor, L, Kohli, M, Weinstein, MC, Singh, S, Pritchard, M, Welin, S, Marteau, F, Gabriel, S, and Feuilly, M
- Published
- 2017
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28. Infrastructure for Detector Research and Development towards the International Linear Collider
- Author
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Aguilar, J., Ambalathankandy, P., Fiutowski, T., Idzik, M., Kulis, Sz., Przyborowski, D., Swientek, K., Bamberger, A., Köhli, M., Lupberger, M., Renz, U., Schumacher, M., Zwerger, Andreas, Calderone, A., Cussans, D. G., Heath, H. F., Mandry, S., Page, R. F., Velthuis, J. J., Attié, D., Calvet, D., Colas, P., Coppolani, X., Degerli, Y., Delagnes, E., Gelin, M., Giomataris, I., Lutz, P., Orsini, F., Rialot, M., Senée, F., Wang, W., Alozy, J., Apostolakis, J., Aspell, P., Bergsma, F., Campbell, M., Formenti, F., Santos, H. Franca, Garcia, E. Garcia, de Gaspari, M., Giudice, P. -A., Grefe, Ch., Grichine, V., Hauschild, M., Ivantchenko, V., Kehrli, A., Kloukinas, K., Linssen, L., Cudie, X. Llopart, Marchioro, A., Musa, L., Ribon, A., Trampitsch, G., Uzhinskiy, V., Anduze, M., Beyer, E., Bonnemaison, A., Boudry, V., Brient, J. -C., Cauchois, A., Clerc, C., Cornat, R., Frotin, M., Gastaldi, F., Jauffret, C., Jeans, D., Karar, A., Mathieu, A., de Freitas, P. Mora, Musat, G., Rougé, A., Ruan, M., Vanel, J. -C., Videau, H., Besson, A., de Masi, G. Claus. R., Doziere, G., Dulinski, W., Goffe, M., Himmi, A., Hu-Guo, Ch., Morel, F., Valin, I., Winter, M., Bonis, J., Callier, S., Cornebise, P., Dulucq, F., Giannelli, M. Faucci, Fleury, J., Guilhem, G., Martin-Chassard, G., de la Taille, Ch., Pöschl, R., Raux, L., Seguin-Moreau, N., Wicek, F., Benyamna, M., Bonnard, J., Cârloganu, C., Fehr, F., Gay, P., Mannen, S., Royer, L., Charpy, A., Da Silva, W., David, J., Dhellot, M., Imbault, D., Ghislain, P., Kapusta, F., Pham, T. Hung, Savoy-Navarro, A., Sefri, R., Dzahini, D., Giraud, J., Grondin, D., Hostachy, J. -Y., Morin, L., Bassignana, D., Pellegrini, G., Lozano, M., Quirion, D., Fernandez, M., Jaramillo, R., Munoz, F. J., Vila, I., Dolezal, Z., Drasal, Z., Kodys, P., Kvasnicka, P., Aplin, S., Bachynska, O., Behnke, T., Behr, J., Dehmelt, K., Engels, J., Gadow, K., Gaede, F., Garutti, E., Göttlicher, P., Gregor, I. -M., Haas, T., Henschel, H., Koetz, U., Lange, W., Libov, V., Lohmann, W., Lutz, B., Mnich, J., Muhl, C., Ohlerich, M., Potylitsina-Kube, N., Prahl, V., Reinecke, M., Roloff, P., Rosemann, Ch., Rubinski, Igor, Schade, P., Schuwalov, S., Sefkow, F., Terwort, M., Volkenborn, R., Kalliopuska, J., Mehtaelae, P., Orava, R., van Remortel, N., Cvach, J., Janata, M., Kvasnicka, J., Marcisovsky, M., Polak, I., Sicho, P., Smolik, J., Vrba, V., Zalesak, J., Bergauer, T., Dragicevic, M., Friedl, M., Haensel, S., Irmler, C., Kiesenhofer, W., Krammer, M., Valentan, M., Piemontese, L., Cotta-Ramusino, A., Bulgheroni, A., Jastrzab, M., Caccia, M., Re, V., Ratti, L., Traversi, G., Dewulf, J. -P., Janssen, X., De Lentdecker, G., Yang, Y., Bryngemark, L., Christiansen, P., Gross, P., Jönsson, L., Ljunggren, M., Lundberg, B., Mjörnmark, U., Oskarsson, A., Richert, T., Stenlund, E., Österman, L., Rummel, S., Richter, R., Andricek, L., Ninkovich, J., Koffmane, Ch., Moser, H. -G., Boisvert, V., Green, B., Green, M. G., Misiejuk, A., Wu, T., Bilevych, Y., Carballo, V. M. Blanco, Chefdeville, M., de Nooij, L., Fransen, M., Hartjes, F., van der Graaf, H., Timmermans, J., Abramowicz, H., Ben-Hamu, Y., Jikhleb, I., Kananov, S., Levy, A., Levy, I., Sadeh, I., Schwartz, R., Stern, A., Goodrick, M. J., Hommels, L. B. A., Ward, R. Shaw. D. R., Daniluk, W., Kielar, E., Kotula, J., Moszczynski, A., Oliwa, K., Pawlik, B., Wierba, W., Zawiejski, L., Bailey, D. S., Kelly, M., Eigen, G., Brezina, Ch., Desch, K., Furletova, J., Kaminski, J., Killenberg, M., Köckner, F., Krautscheid, T., Krüger, H., Reuen, L., Wienemann, P., Zimmermann, R., Zimmermann, S., Bartsch, V., Postranecky, M., Warren, M., Wing, M., Corrin, E., Haas, D., Pohl, M., Diener, R., Fischer, P., Peric, I., Kaukher, A., Schäfer, O., Schröder, H., Wurth, R., Zarnecki, A. F., Aguilar, J., Ambalathankandy, P., Fiutowski, T., Idzik, M., Kulis, Sz., Przyborowski, D., Swientek, K., Bamberger, A., Köhli, M., Lupberger, M., Renz, U., Schumacher, M., Zwerger, Andreas, Calderone, A., Cussans, D. G., Heath, H. F., Mandry, S., Page, R. F., Velthuis, J. J., Attié, D., Calvet, D., Colas, P., Coppolani, X., Degerli, Y., Delagnes, E., Gelin, M., Giomataris, I., Lutz, P., Orsini, F., Rialot, M., Senée, F., Wang, W., Alozy, J., Apostolakis, J., Aspell, P., Bergsma, F., Campbell, M., Formenti, F., Santos, H. Franca, Garcia, E. Garcia, de Gaspari, M., Giudice, P. -A., Grefe, Ch., Grichine, V., Hauschild, M., Ivantchenko, V., Kehrli, A., Kloukinas, K., Linssen, L., Cudie, X. Llopart, Marchioro, A., Musa, L., Ribon, A., Trampitsch, G., Uzhinskiy, V., Anduze, M., Beyer, E., Bonnemaison, A., Boudry, V., Brient, J. -C., Cauchois, A., Clerc, C., Cornat, R., Frotin, M., Gastaldi, F., Jauffret, C., Jeans, D., Karar, A., Mathieu, A., de Freitas, P. Mora, Musat, G., Rougé, A., Ruan, M., Vanel, J. -C., Videau, H., Besson, A., de Masi, G. Claus. R., Doziere, G., Dulinski, W., Goffe, M., Himmi, A., Hu-Guo, Ch., Morel, F., Valin, I., Winter, M., Bonis, J., Callier, S., Cornebise, P., Dulucq, F., Giannelli, M. Faucci, Fleury, J., Guilhem, G., Martin-Chassard, G., de la Taille, Ch., Pöschl, R., Raux, L., Seguin-Moreau, N., Wicek, F., Benyamna, M., Bonnard, J., Cârloganu, C., Fehr, F., Gay, P., Mannen, S., Royer, L., Charpy, A., Da Silva, W., David, J., Dhellot, M., Imbault, D., Ghislain, P., Kapusta, F., Pham, T. Hung, Savoy-Navarro, A., Sefri, R., Dzahini, D., Giraud, J., Grondin, D., Hostachy, J. -Y., Morin, L., Bassignana, D., Pellegrini, G., Lozano, M., Quirion, D., Fernandez, M., Jaramillo, R., Munoz, F. J., Vila, I., Dolezal, Z., Drasal, Z., Kodys, P., Kvasnicka, P., Aplin, S., Bachynska, O., Behnke, T., Behr, J., Dehmelt, K., Engels, J., Gadow, K., Gaede, F., Garutti, E., Göttlicher, P., Gregor, I. -M., Haas, T., Henschel, H., Koetz, U., Lange, W., Libov, V., Lohmann, W., Lutz, B., Mnich, J., Muhl, C., Ohlerich, M., Potylitsina-Kube, N., Prahl, V., Reinecke, M., Roloff, P., Rosemann, Ch., Rubinski, Igor, Schade, P., Schuwalov, S., Sefkow, F., Terwort, M., Volkenborn, R., Kalliopuska, J., Mehtaelae, P., Orava, R., van Remortel, N., Cvach, J., Janata, M., Kvasnicka, J., Marcisovsky, M., Polak, I., Sicho, P., Smolik, J., Vrba, V., Zalesak, J., Bergauer, T., Dragicevic, M., Friedl, M., Haensel, S., Irmler, C., Kiesenhofer, W., Krammer, M., Valentan, M., Piemontese, L., Cotta-Ramusino, A., Bulgheroni, A., Jastrzab, M., Caccia, M., Re, V., Ratti, L., Traversi, G., Dewulf, J. -P., Janssen, X., De Lentdecker, G., Yang, Y., Bryngemark, L., Christiansen, P., Gross, P., Jönsson, L., Ljunggren, M., Lundberg, B., Mjörnmark, U., Oskarsson, A., Richert, T., Stenlund, E., Österman, L., Rummel, S., Richter, R., Andricek, L., Ninkovich, J., Koffmane, Ch., Moser, H. -G., Boisvert, V., Green, B., Green, M. G., Misiejuk, A., Wu, T., Bilevych, Y., Carballo, V. M. Blanco, Chefdeville, M., de Nooij, L., Fransen, M., Hartjes, F., van der Graaf, H., Timmermans, J., Abramowicz, H., Ben-Hamu, Y., Jikhleb, I., Kananov, S., Levy, A., Levy, I., Sadeh, I., Schwartz, R., Stern, A., Goodrick, M. J., Hommels, L. B. A., Ward, R. Shaw. D. R., Daniluk, W., Kielar, E., Kotula, J., Moszczynski, A., Oliwa, K., Pawlik, B., Wierba, W., Zawiejski, L., Bailey, D. S., Kelly, M., Eigen, G., Brezina, Ch., Desch, K., Furletova, J., Kaminski, J., Killenberg, M., Köckner, F., Krautscheid, T., Krüger, H., Reuen, L., Wienemann, P., Zimmermann, R., Zimmermann, S., Bartsch, V., Postranecky, M., Warren, M., Wing, M., Corrin, E., Haas, D., Pohl, M., Diener, R., Fischer, P., Peric, I., Kaukher, A., Schäfer, O., Schröder, H., Wurth, R., and Zarnecki, A. F.
- Abstract
The EUDET-project was launched to create an infrastructure for developing and testing new and advanced detector technologies to be used at a future linear collider. The aim was to make possible experimentation and analysis of data for institutes, which otherwise could not be realized due to lack of resources. The infrastructure comprised an analysis and software network, and instrumentation infrastructures for tracking detectors as well as for calorimetry., Comment: 54 pages, 48 pictures
- Published
- 2012
29. 720PD - Long term efficacy and QOL data of chemohormonal therapy (C-HT) in low and high volume hormone naïve metastatic prostate cancer (PrCa): E3805 CHAARTED trial
- Author
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Sweeney, C., Chen, Y.-H., Liu, G., Carducci, M., Jarrard, D., Eisenberger, M., Wong, Y.-N., Patrick-Miller, L., Hahn, N., Kohli, M., Conney, M., Dreicer, R., Vogelzang, N.J., Picus, J., Shevrin, D., Hussain, M., Garcia, J., and Dipaola, R.
- Published
- 2016
- Full Text
- View/download PDF
30. PMD46 - ECONOMIC EVALUATIONS OF MEDICAL DEVICES IN CANADA: LENSX® FEMTOSECOND LASER-ASSISTED CATARACT SURGERY
- Author
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Lee, A., Keith, M.S., Prasad, S., Kohli, M., Ferreira, A., and Bouchet, C.
- Published
- 2016
- Full Text
- View/download PDF
31. Nuclear dynamics during germination, conidiation, and hyphal fusion of Fusarium oxysporum
- Author
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Ruiz-Roldán, M.C., Köhli, M., Roncero, M. Isabel G., Philippsen, P., Di Pietro, A., Espeso, Eduardo A., Ruiz-Roldán, M.C., Köhli, M., Roncero, M. Isabel G., Philippsen, P., Di Pietro, A., and Espeso, Eduardo A.
- Abstract
In many fungal pathogens, infection is initiated by conidial germination. Subsequent stages involve germ tube elongation, conidiation, and vegetative hyphal fusion (anastomosis). Here, we used live-cell fluorescence to study the dynamics of green fluorescent protein (GFP)- and cherry fluorescent protein (ChFP)-labeled nuclei in the plant pathogen Fusarium oxysporum. Hyphae of F. oxysporum have uninucleated cells and exhibit an acropetal nuclear pedigree, where only the nucleus in the apical compartment is mitotically active. In contrast, conidiation follows a basopetal pattern, whereby mononucleated microconidia are generated by repeated mitotic cycles of the subapical nucleus in the phialide, followed by septation and cell abscission. Vegetative hyphal fusion is preceded by directed growth of the fusion hypha toward the receptor hypha and followed by a series of postfusion nuclear events, including mitosis of the apical nucleus of the fusion hypha, migration of a daughter nucleus into the receptor hypha, and degradation of the resident nucleus. These previously unreported patterns of nuclear dynamics in F. oxysporum could be intimately related to its pathogenic lifestyle.
- Published
- 2010
32. 756O - Chemohormonal Therapy Versus Hormonal Therapy for Hormone Naïve High Volume Newly Metastatic Prostate Cancer (Prca): Ecog Led Phase III Randomized Trial
- Author
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Sweeney, C., Chen, Y., Carducci, M., Liu, G., Jarrard, D., Eisenberger, M., Wong, Y., Hahn, N.M., Kohli, M., Vogelzang, N., Cooney, M., Dreicer, R., Picus, J., Shevrin, D., Hussain, M., Garcia, J., and Dipaola, R.
- Published
- 2014
- Full Text
- View/download PDF
33. PIN25 - Public And Private Payer Perspectives On The Net Cost Of Implementation Of 13-Valent Pneumococcal Conjugate Vaccine (PCV13) Compared With 7-Valent Pneumococcal Conjugate Vaccine (PCV7) To In The United States
- Author
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Kohli, M., Maschio, M., Farkouh, R.A., McGarry, L., Strutton, D.R., and Weinstein, M.C.
- Published
- 2014
- Full Text
- View/download PDF
34. PIH55 - Do Utility Values Reflect Lost Income And The Full Opportunity Cost Of Work Loss?
- Author
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Fust, K., Kohli, M., Ball, G., Kawai, K., Acosta, C.J., and Weinstein, M.C.
- Published
- 2014
- Full Text
- View/download PDF
35. PHP132 - Great ROC! So What? Bringing Predictive Modeling And Risk Scores Into The Physicians' Office
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Kohli, M., Bancroft, T., Riedel, A., Drollinger, K., and Wilson, J.
- Published
- 2013
- Full Text
- View/download PDF
36. PIH22 EVALUATING THE COST-EFFECTIVENESS OF CERVICAL CANCER SCREENING AND HUMAN PAPILLOMAVIRUS VACCINATION STRATEGIES USING A MATHEMATICAL MODEL
- Author
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Taylor, DC, Pawar, V, Gilmore, K, Sanon, M, Kruzikas, D, Kohli, M, Arondekar, B, Demarteau, N, and Weinstein, M
- Published
- 2010
- Full Text
- View/download PDF
37. Potential Cost Impact in the Netherlands Due to Different Waning Patterns of Cervical Cancer Vaccines
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Rogoza, R.M., Debicki, D., and Kohli, M.
- Published
- 2008
- Full Text
- View/download PDF
38. PCN3 POTENTIAL IMPACT OF WANING OF VACCINE-INDUCED IMMUNITY AGAINST HUMAN PAPILLOMAVIRUS 16/18
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Goldie, S, Kohli, M, Grima, D, Weinstein, MC, Wright, T, Bosch, F, and Franco, E
- Published
- 2004
- Full Text
- View/download PDF
39. PIN37 A COMPREHENSIVE NATURAL HISTORY MODEL OF HUMAN PAPILLOMAVIRUS (HPV) INFECTION AND CERVICAL CANCER: POTENTIAL IMPACT OF AN HPV 16/18 VACCINE
- Author
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Goldie, S, Grima, D, Kohli, M, Weinstein, M, Wright, T, and Franco, E
- Published
- 2002
- Full Text
- View/download PDF
40. Bhorugram (India): revisited A 4 year follow-up of a computer-based information system for distributed MCH services
- Author
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Singh, A.K., Kohli, M., Trell, E., Wigertz, O., and Kohli, S.
- Published
- 1997
- Full Text
- View/download PDF
41. Sensing Area‐Average Snow Water Equivalent with Cosmic‐Ray Neutrons: The Influence of Fractional Snow Cover
- Author
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Paul Schattan, Markus Köhli, Gabriele Baroni, Sascha E. Oswald, Martin Schrön, Schattan, P., Köhli, M., Schrön, M., Baroni, G., and Oswald, S. E.
- Subjects
010504 meteorology & atmospheric sciences ,0207 environmental engineering ,Cosmic ray ,02 engineering and technology ,Snow ,Atmospheric sciences ,Water equivalent ,01 natural sciences ,Spatial heterogeneity ,13. Climate action ,ddc:550 ,Environmental science ,Neutron ,Institut für Geowissenschaften ,020701 environmental engineering ,Snow cover ,snow water equivalent, cosmic-ray neutron sensing, neutron simulations ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
Cosmic‐ray neutron sensing (CRNS) is a promising non‐invasive technique to estimate snow water equivalent (SWE) over large areas. In contrast to preliminary studies focusing on shallow snow conditions (SWE < 130 mm), more recently the method was shown experimentally to be sensitive also to deeper snowpacks providing the basis for its use at mountain experimental sites. However, hysteretic neutron response has been observed for complex snow cover including patchy snow‐free areas. In the present study we aimed to understand and support the experimental findings using a comprehensive neutron modeling approach. Several simulations have been set up in order to disentangle the effect on the signal of different land surface characteristics and to reproduce multiple observations during periods of snow melt and accumulation. To represent the actual land surface heterogeneity and the complex snow cover, the model used data from terrestrial laser scanning. The results show that the model was able to accurately reproduce the CRNS signal and particularly the hysteresis effect during accumulation and melting periods. Moreover, the sensor footprint was found to be anisotropic and affected by the spatial distribution of liquid water and snow as well as by the topography of the nearby mountains. Under fully snow‐covered conditions the CRNS is able to accurately estimate SWE without prior knowledge about snow density profiles or other spatial anomalies. These results provide new insights into the characteristics of the detected neutron signal in complex terrain and support the use of CRNS for long‐term snow monitoring in high elevated mountain environments.
- Published
- 2019
42. Arduino-Based Readout Electronics for Nuclear and Particle Physics.
- Author
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Köhli M, Weimar J, Schmidt S, Schmidt FP, Lambertz A, Weber L, Kaminski J, and Schmidt U
- Abstract
Open Hardware-based microcontrollers, especially the Arduino platform, have become a comparably easy-to-use tool for rapid prototyping and implementing creative solutions. Such devices in combination with dedicated front-end electronics can offer low-cost alternatives for student projects, slow control and independently operating small-scale instrumentation. The capabilities can be extended to data taking and signal analysis at mid-level rates. Two detector realizations are presented, which cover the readouts of proportional counter tubes and of scintillators or wavelength-shifting fibers with silicon photomultipliers (SiPMs). The SiPMTrigger realizes a small-scale design for coincidence readout of SiPMs as a trigger or veto detector. It consists of a custom mixed signal front-end board featuring signal amplification, discrimination and a coincidence unit for rates of up to 200 kHz. The nCatcher transforms an Arduino Nano to a proportional counter readout with pulse shape analysis: time over threshold measurement and a 10-bit analog-to-digital converter for pulse heights. The device is suitable for low-to-medium-rate environments up to 5 kHz, where a good signal-to-noise ratio is crucial. We showcase the monitoring of thermal neutrons. For data taking and slow control, a logger board is presented that features an SD card and GSM/LoRa interface.
- Published
- 2024
- Full Text
- View/download PDF
43. Monitoring Irrigation in Small Orchards with Cosmic-Ray Neutron Sensors.
- Author
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Brogi C, Pisinaras V, Köhli M, Dombrowski O, Hendricks Franssen HJ, Babakos K, Chatzi A, Panagopoulos A, and Bogena HR
- Abstract
Due to their unique characteristics, cosmic-ray neutron sensors (CRNSs) have potential in monitoring and informing irrigation management, and thus optimising the use of water resources in agriculture. However, practical methods to monitor small, irrigated fields with CRNSs are currently not available and the challenges of targeting areas smaller than the CRNS sensing volume are mostly unaddressed. In this study, CRNSs are used to continuously monitor soil moisture (SM) dynamics in two irrigated apple orchards (Agia, Greece) of ~1.2 ha. The CRNS-derived SM was compared to a reference SM obtained by weighting a dense sensor network. In the 2021 irrigation period, CRNSs could only capture the timing of irrigation events, and an ad hoc calibration resulted in improvements only in the hours before irrigation (RMSE between 0.020 and 0.035). In 2022, a correction based on neutron transport simulations, and on SM measurements from a non-irrigated location, was tested. In the nearby irrigated field, the proposed correction improved the CRNS-derived SM (from 0.052 to 0.031 RMSE) and, most importantly, allowed for monitoring the magnitude of SM dynamics that are due to irrigation. The results are a step forward in using CRNSs as a decision support system in irrigation management.
- Published
- 2023
- Full Text
- View/download PDF
44. Nuclear dynamics during germination, conidiation, and hyphal fusion of Fusarium oxysporum.
- Author
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Ruiz-Roldán MC, Köhli M, Roncero MI, Philippsen P, Di Pietro A, and Espeso EA
- Subjects
- Cell Polarity, Fluorescent Dyes metabolism, Fusarium growth & development, Fusarium ultrastructure, Green Fluorescent Proteins metabolism, Histones metabolism, Hyphae growth & development, Solanum lycopersicum microbiology, Solanum lycopersicum ultrastructure, Mitosis, Plant Diseases microbiology, Plant Roots microbiology, Plant Roots ultrastructure, Spores, Fungal cytology, Time Factors, Cell Nucleus metabolism, Fusarium cytology, Fusarium physiology, Germination physiology, Hyphae cytology, Hyphae physiology, Spores, Fungal physiology
- Abstract
In many fungal pathogens, infection is initiated by conidial germination. Subsequent stages involve germ tube elongation, conidiation, and vegetative hyphal fusion (anastomosis). Here, we used live-cell fluorescence to study the dynamics of green fluorescent protein (GFP)- and cherry fluorescent protein (ChFP)-labeled nuclei in the plant pathogen Fusarium oxysporum. Hyphae of F. oxysporum have uninucleated cells and exhibit an acropetal nuclear pedigree, where only the nucleus in the apical compartment is mitotically active. In contrast, conidiation follows a basopetal pattern, whereby mononucleated microconidia are generated by repeated mitotic cycles of the subapical nucleus in the phialide, followed by septation and cell abscission. Vegetative hyphal fusion is preceded by directed growth of the fusion hypha toward the receptor hypha and followed by a series of postfusion nuclear events, including mitosis of the apical nucleus of the fusion hypha, migration of a daughter nucleus into the receptor hypha, and degradation of the resident nucleus. These previously unreported patterns of nuclear dynamics in F. oxysporum could be intimately related to its pathogenic lifestyle.
- Published
- 2010
- Full Text
- View/download PDF
45. Growth-speed-correlated localization of exocyst and polarisome components in growth zones of Ashbya gossypii hyphal tips.
- Author
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Köhli M, Galati V, Boudier K, Roberson RW, and Philippsen P
- Subjects
- Cell Polarity, Eremothecium metabolism, Fungal Proteins analysis, Fungal Proteins metabolism, Hyphae metabolism, Hyphae ultrastructure, Eremothecium growth & development, Hyphae growth & development
- Abstract
We use the fungus Ashbya gossypii to investigate how its polar growth machinery is organized to achieve sustained hyphal growth. In slowly elongating hyphae exocyst, cell polarity and polarisome proteins permanently localize as cortical cap at hyphal tips, thus defining the zone of secretory vesicle fusion. In tenfold faster growing hyphae, this zone is only slightly enlarged demonstrating a capacity of hyphal growth zones to increase rates of vesicle processing to reach higher speeds. Concomitant with this increase, vesicles accumulate as spheroid associated with the tip cortex, indicating that a Spitzenkörper forms in fast hyphae. We also found spheroid-like accumulations for the exocyst components AgSec3, AgSec5, AgExo70 and the polarisome components AgSpa2, AgBni1 and AgPea2 (but not AgBud6 or cell polarity factors such as AgCdc42 or AgBem1). The localization of AgSpa2, AgPea2 and AgBni1 depend on each other but only marginally on AgBud6, as concluded from a set of deletions. Our data define three conditions to achieve fast growth at hyphal tips: permanent presence of the polarity machinery in a confined cortical area, organized accumulation of vesicles and a subset of polarity components close to this area, and spatial separation of the zones of exocytosis (tip front) and endocytosis (tip rim).
- Published
- 2008
- Full Text
- View/download PDF
46. The function of two closely related Rho proteins is determined by an atypical switch I region.
- Author
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Köhli M, Buck S, and Schmitz HP
- Subjects
- Blotting, Western, Fungal Proteins chemistry, Fungal Proteins genetics, GTP-Binding Proteins genetics, GTPase-Activating Proteins genetics, Gene Duplication, Histidine genetics, Histidine metabolism, Protein Binding, Saccharomycetales genetics, Substrate Specificity, Two-Hybrid System Techniques, Tyrosine genetics, Tyrosine metabolism, Fungal Proteins metabolism, GTP-Binding Proteins metabolism, GTPase-Activating Proteins metabolism, Saccharomycetales metabolism
- Abstract
We show here that the encoded proteins of the two duplicated RHO1 genes from the filamentous fungus Ashbya gossypii, AgRHO1a and AgRHO1b have functionally diverged by unusual mutation of the conserved switch I region. Interaction studies and in vitro assays suggest that a different regulation by the two GTPase activating proteins (GAPs) AgLrg1 and AgSac7 contributes to the functional differences. GAP-specificity and protein function is determined to a large part by a single position in the switch I region of the two Rho1 proteins. In AgRho1b, this residue is a tyrosine that is conserved among the Rho-protein family, whereas AgRho1a carries an atypical histidine at the same position. Mutation of this histidine to a tyrosine changes GAP-specificity, protein function and localization of AgRho1a. Furthermore, it enables the mutated allele to complement the lethality of an AgRHO1b deletion. In summary, our findings show that a simple mutation in the switch I region of a GTP-binding protein can change its affinity towards its GAPs, which finally leads to a decoupling of very similar protein function without impairing effector interaction.
- Published
- 2008
- Full Text
- View/download PDF
47. From function to shape: a novel role of a formin in morphogenesis of the fungus Ashbya gossypii.
- Author
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Schmitz HP, Kaufmann A, Köhli M, Laissue PP, and Philippsen P
- Subjects
- Actins metabolism, Amino Acids genetics, Amino Acids metabolism, Biological Transport, Cell Nucleus genetics, Cell Nucleus metabolism, Cell Polarity, Cell Proliferation, Fungal Proteins classification, Fungal Proteins genetics, Gene Deletion, Genes, Lethal genetics, Hyphae, Microfilament Proteins genetics, Microfilament Proteins metabolism, Microscopy, Atomic Force, Mutation genetics, Saccharomyces cerevisiae cytology, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Saccharomycetales genetics, cdc42 GTP-Binding Protein genetics, cdc42 GTP-Binding Protein metabolism, Fungal Proteins metabolism, Morphogenesis, Saccharomycetales cytology, Saccharomycetales metabolism
- Abstract
Morphogenesis of filamentous ascomycetes includes continuously elongating hyphae, frequently emerging lateral branches, and, under certain circumstances, symmetrically dividing hyphal tips. We identified the formin AgBni1p of the model fungus Ashbya gossypii as an essential factor in these processes. AgBni1p is an essential protein apparently lacking functional overlaps with the two additional A. gossypii formins that are nonessential. Agbni1 null mutants fail to develop hyphae and instead expand to potato-shaped giant cells, which lack actin cables and thus tip-directed transport of secretory vesicles. Consistent with the essential role in hyphal development, AgBni1p locates to tips, but not to septa. The presence of a diaphanous autoregulatory domain (DAD) indicates that the activation of AgBni1p depends on Rho-type GTPases. Deletion of this domain, which should render AgBni1p constitutively active, completely changes the branching pattern of young hyphae. New axes of polarity are no longer established subapically (lateral branching) but by symmetric divisions of hyphal tips (tip splitting). In wild-type hyphae, tip splitting is induced much later and only at much higher elongation speed. When GTP-locked Rho-type GTPases were tested, only the young hyphae with mutated AgCdc42p split at their tips, similar to the DAD deletion mutant. Two-hybrid experiments confirmed that AgBni1p interacts with GTP-bound AgCdc42p. These data suggest a pathway for transforming one axis into two new axes of polar growth, in which an increased activation of AgBni1p by a pulse of activated AgCdc42p stimulates additional actin cable formation and tip-directed vesicle transport, thus enlarging and ultimately splitting the polarity site.
- Published
- 2006
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