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138 results on '"Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD)"'

1. In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line

Author: Teuta Eljezi, Pierre Pinta, Marie-Christine Chagnon, Damien Richard, Jean-Michel Chezal, Jérémy Pinguet, Valérie Sautou, Gaël Grimandi, Emmanuel Moreau, Imagerie Moléculaire et Stratégies Théranostiques - Clermont Auvergne ( IMoST ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Clermont Auvergne ( UCA ), CHU Clermont-Ferrand, Laboratoire d'ingénierie osteo-articulaire et dentaire ( LIOAD ), Université de Nantes ( UN ) -IFR26-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Service de Pharmacologie Médicale [Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], Equipe NuTox (LNC - U1231) ( NUTOX ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Caractérisation et sécurité biologique des surfaces nanostructurées ( C-BIOSENSS ), Université d'Auvergne - Clermont-Ferrand I ( UdA ), Matériaux d'intérêt biologique, Université de Nantes ( UN ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Service Pharmacie [CHU Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand-CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Louise Michel [Clermont-Ferrand], Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche en Gestion et en Economie (IREGE), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Service de Pharmacologie Médicale [CHU Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Caractérisation et sécurité biologique des surfaces nanostructurées (C-BIOSENSS), Université d'Auvergne - Clermont-Ferrand I (UdA), Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), CHU Louise Michel [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand-CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Estaing [Clermont-Ferrand], Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), and Jehan, Frederic
Subjects: 0301 basic medicine, Environmental Engineering, Metabolite synthesis, Biocompatibility, Cell Survival, Cytotoxicity, Health, Toxicology and Mutagenesis, Metabolite, Phthalic Acids, In Vitro Techniques, DEHP-alternative plasticizers, 010501 environmental sciences, 01 natural sciences, [ SDE ] Environmental Sciences, Mice, 03 medical and health sciences, chemistry.chemical_compound, Phthalates, Plasticizers, In vivo, Diethylhexyl Phthalate, Animals, Environmental Chemistry, Organic chemistry, Polyvinyl Chloride, Cells, Cultured, 0105 earth and related environmental sciences, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Chromatography, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Public Health, Environmental and Occupational Health, Plasticizer, Primary metabolite, Esters, General Medicine, General Chemistry, Fibroblasts, Pollution, In vitro, 3. Good health, Phthalic acid, 030104 developmental biology, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, chemistry
Abstract: IF 4.208; International audience; Phthalic acid esters have been widely used to improve the plasticity of PVC medical devices. They carry a high exposure risk for both humans and the environment in clinical situations. Our study focuses on the cytotoxicity of alternative plasticizers. Postulated primary metabolites were synthesized, not being commercially available. Cytotoxicity assays were performed on L929 murine cells according to the ISO-EN 10993-5 standard design for the biocompatibility of medical devices. The tested concentrations of plasticizers (0.01, 0.05 and 0.1 mg/ml) covered the range likely to be found in biological fluids coming into direct contact with the medical devices. DEHP, DINP and DINCH were cytotoxic at the highest concentration (0.1 mg/ml) for 7 days of exposure. Their corresponding metabolites were found to be more cytotoxic, for the same concentration. By contrast, TOTM and its corresponding metabolite MOTM were not found to be cytotoxic. DEHA showed no cytotoxicity, but its corresponding monoester (MEHA) produced a cytotoxic effect at 0.05 mg/ml. In clinical situations, medical devices can release plasticizers, which can come into contact with patients. In vivo, the plasticizers are quickly transformed into primary metabolites. It is therefore important to measure the effects of both the plasticizers and their corresponding metabolites. Standard first-line cytotoxicity assays should be performed to ensure biocompatibility.
Published: 2017

2. Regional and latitudinal patterns of soft-bottom macrobenthic invertebrates along French coasts: Results from the RESOMAR database

Author: Nicolas Desroy, Michel Le Duff, Jean-Claude Dauvin, Antoine Grémare, Céline Houbin, Franck Gentil, Frédéric Olivier, Jérôme Jourde, Olivier Gauthier, Xavier de Montaudouin, Sandrine Laurand, Hugues Blanchet, Nicolas Lavesque, Céline Labrune, Vincent M.P. Bouchet, Jacques Grall, Francis Orvain, Eric Thiébaut, Pierre-Guy Sauriau, Paulo Bonifácio, Régis Gallon, Guy Bachelet, Vincent Le Garrec, Laurent Guerin, Institut national des sciences et techniques de la mer (INTECHMER), Conservatoire National des Arts et Métiers [CNAM] (CNAM), Laboratoire Universitaire des Sciences Appliquées de Cherbourg (LUSAC), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecogéochimie des environnements benthiques (LECOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Morphodynamique Continentale et Côtière (M2C), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Environnement Ressource Bretagne Nord (LERBN), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Station marine Dinard, Centre National de la Recherche Scientifique (CNRS), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Environnements et Paléoenvironnements OCéaniques (EPOC), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN), Station de Biologie Marine de Concarneau, Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE), Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN), Normandie Université (NU), ANR-13-BSV7-0006, Agence Nationale de la Recherche, Centre National de la Recherche Scientifique, ANR-13-BSV7-0006,BenthoVal,Valorisation de la base de données Macrofaune benthique du RESOMAR : évaluation la dynamique spatio-temporelle des communautés benthique côtières et de l'État Écologique des habitats dans le cadre de la DCSMM et de la DCE(2013), Institut des Sciences et Techniques de la Mer ( INTECHMER ), Conservatoire National des Arts et Métiers [CNAM] ( CNAM ), Université de Brest ( UBO ), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux ( EPOC ), Observatoire aquitain des sciences de l'univers ( OASU ), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -École pratique des hautes études ( EPHE ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Ecogéochimie des environnements benthiques ( LECOB ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Université du Littoral Côte d'Opale ( ULCO ), Morphodynamique Continentale et Côtière ( M2C ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ), CRESCO, Station IFREMER, CRESCO, Station IFREMER, 38 rue du Port-Blanc, BP 80108, 35801 Dinard Cedex, France, Station biologique de Roscoff [Roscoff] ( SBR ), Institut de Physique de Rennes ( IPR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Centre National de la Recherche Scientifique ( CNRS ), LIttoral ENvironnement et Sociétés - UMR 7266 ( LIENSs ), Université de La Rochelle ( ULR ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire des Sciences de l'Environnement Marin (LEMAR) ( LEMAR ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Brest ( UBO ) -Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ) -Institut Universitaire Européen de la Mer ( IUEM ), Institut de Recherche pour le Développement ( IRD ) -Université de Brest ( UBO ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université de Brest ( UBO ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ), Institut Universitaire Européen de la Mer ( IUEM ), Institut de Recherche pour le Développement ( IRD ) -Université de Brest ( UBO ) -Centre National de la Recherche Scientifique ( CNRS ), Environnements et Paléoenvironnements OCéaniques ( EPOC ), Biologie des Organismes et Ecosystèmes Aquatiques ( BOREA ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Muséum National d'Histoire Naturelle ( MNHN ) -Institut de Recherche pour le Développement ( IRD ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université des Antilles ( UA ), Université Pierre et Marie Curie, Univ Paris 06 ( Sorbonne University ), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique ( ONIRIS ), Laboratoire d'ingénierie osteo-articulaire et dentaire ( LIOAD ), Université de Nantes ( UN ) -IFR26-Institut National de la Santé et de la Recherche Médicale ( INSERM ), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord]), LITTORAL (LITTORAL), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Adaptation et diversité en milieu marin (ADMM), Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Fédération de recherche de Roscoff (FR2424), Centre De Recherche et d'Enseignement sur les Systèmes Côtiers (CRESCO), LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Université de Brest (UBO), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université du Littoral Côte d'Opale (ULCO), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut de Physique de Rennes (IPR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut Universitaire Européen de la Mer (IUEM), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO), Sorbonne Université (SU), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO), Centre National de la Recherche Scientifique (CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut national des sciences de l'Univers (INSU - CNRS), HESAM Université (HESAM)-HESAM Université (HESAM), Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), and LIttoral ENvironnement et Sociétés - UMR 7266 (LIENSs)
Subjects: 0106 biological sciences, Latitudinal pattern, Aquatic Science, Oceanography, computer.software_genre, 010603 evolutionary biology, 01 natural sciences, Mediterranean sea, Hotspot (geology), zoobenthos, 14. Life underwater, species richness, Ecology, Evolution, Behavior and Systematics, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography, [PHYS]Physics [physics], [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, [ PHYS ] Physics [physics], Database, Soft-bottom, Phylum, 010604 marine biology & hydrobiology, ACL, 15. Life on land, Zoobenthos, Regional pattern, Geography, Taxon, Habitat, DISCOVERY, Species richness, France, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, computer, Bay, Global biodiversity
Abstract: International audience; This study aims to describe the patterns of soft bottom macrozoobenthic richness along French coasts. It is based on a collaborative database developed by the “Réseau des Stations et Observatoires Marins” (RESOMAR). We investigated patterns of species richness in sublittoral soft bottom habitats (EUNIS level 3) at two different spatial scales: 1) seaboards: English Channel, Bay of Biscay and Mediterranean Sea and 2) 0.5° latitudinal and longitudinal grid. Total observed richness, rarefaction curves and three incidence-based richness estimators (Chao2, ICE and Jacknife1) were used to compare soft bottom habitats species richness in each seaboard. Overall, the Mediterranean Sea has the highest richness and despite higher sampling effort, the English Channel hosts the lowest number of species. The distribution of species occurrence within and between seaboards was assessed for each major phylum using constrained rarefaction curves. The Mediterranean Sea hosts the highest number of exclusive species. In pairwise comparisons, it also shares a lower proportion of taxa with the Bay of Biscay (34.1%) or the English Channel (27.6%) than that shared between these two seaboards (49.7%). Latitudinal species richness patterns along the Atlantic and English Channel coasts were investigated for each major phylum using partial LOESS regression controlling for sampling effort. This showed the existence of a bell-shaped latitudinal pattern, highlighting Brittany as a hotspot for macrobenthic richness at the confluence of two biogeographic provinces.
Published: 2017

3. Protein mapping of calcium carbonate biominerals by immunogold

Author: Boaz Pokroy, Gilles Luquet, Klaas de Groot, Pierre Layrolle, Frédéric Marin, Faculty of Science and Technology, Biomaterials Science and Technology, Layrolle, Pierre, Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Department of Materials Engineering, Technion - Israel Institute of Technology [Haifa], Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute for Biomedical Technology, University of Twente, Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'ingénierie osteo-articulaire et dentaire ( LIOAD ), Université de Nantes ( UN ) -IFR26-Institut National de la Santé et de la Recherche Médicale ( INSERM ), University of Twente [Netherlands], Biogéosciences [UMR 6282] [Dijon] (BGS), and Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)
Subjects: MESH : Models, Chemical, MESH : Molecular Sequence Data, MESH: Sequence Homology, Amino Acid, MESH : Calcium Carbonate, MESH : Immunohistochemistry, MESH : Aspartic Acid, MESH: Trypsin, MESH: Amino Acid Sequence, Matrix (biology), 01 natural sciences, MESH: Aspartic Acid, MESH : Proteins, chemistry.chemical_compound, Trypsin, MESH: Animals, MESH: Proteins, Peptide sequence, MESH: Crystallization, chemistry.chemical_classification, 0303 health sciences, Caspartin, biology, MESH : Amino Acid Sequence, MESH : Pepsin A, MESH: Models, Chemical, Immunogold labelling, Immunohistochemistry, MESH: Mollusca, MESH : Sequence Homology, Amino Acid, Amino acid, Biochemistry, MESH: Calcium Carbonate, Mechanics of Materials, MESH : Crystallization, MESH: Pepsin A, SEM, MESH : Edetic Acid, Crystallization, MESH : Mollusca, Calcium carbonate, Proteinaceous extracellular matrix, MESH: Edetic Acid, Molecular Sequence Data, Biophysics, Bioengineering, 010402 general chemistry, Biomaterials, 03 medical and health sciences, Animals, Amino Acid Sequence, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Edetic Acid, 030304 developmental biology, Aspartic Acid, Viral matrix protein, MESH: Molecular Sequence Data, Sequence Homology, Amino Acid, MESH : Solubility, Back-scattered electrons, Surface treatmen, Proteins, MESH: Immunohistochemistry, IR-78873, [ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterials, Pepsin A, 0104 chemical sciences, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, MESH: Solubility, chemistry, Models, Chemical, Solubility, Polyclonal antibodies, Mollusca, Ceramics and Composites, biology.protein, MESH : Animals, MESH : Trypsin, Immunogold
Abstract: The construction of metazoan calcium carbonate skeletons is finely regulated by a proteinaceous extracellular matrix, which remains embedded within the exoskeleton. In spite of numerous biochemical studies, the precise localization of skeletal proteins has remained for a long time as an elusive goal. In this paper, we describe a technique for visualizing shell matrix proteins on the surface of calcium carbonate crystals or within the biominerals. The technique is as follows: freshly broken pieces of biominerals or NaOCl then EDTA-etched polished surfaces are incubated with an antibody elicited against one matrix protein, then with a secondary gold-coupled antibody. After silver enhancement, the samples are subsequently observed with scanning electron microscopy by using back-scattered electron mode. In the present case, the technique is applied to a particular example, the calcitic prisms that compose the outer shell layer of the mediterranean fan mussel Pinna nobilis. One major soluble protein, caspartin, which was identified recently, was partly de novo sequenced after enzymatic digestions. A polyclonal antibody raised against caspartin was used for its localization within and on the prisms. The immunogold localization indicated that caspartin surrounds the calcitic prisms, but is also dispersed within the biominerals. This example illustrates the deep impact of the technique on the definition of intracrystalline versus intercrystalline matrix proteins. Furthermore, it is an important tool for assigning a putative function to a matrix protein of interest.
Published: 2007

4. Re: Toward a consensus view in the management of acute facial injuries during the COVID-19 pandemic

Author: Pierre Corre, R. Deransy, Hélios Bertin, C. Koudougou, F. Marion, Jehan, Frederic, Service de chirurgie maxillo-faciale et stomatologie [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Sarcomes osseux et remodelage des tissus calcifiés - Phy-Os [Nantes - INSERM U1238] (Phy-Os), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes), Chirurgie Maxillo-faciale et Stomatologie [Hôpital Privé du Confluent], Hôpital privé du Confluent [Nantes], Chirurgie Maxillo-faciale et Stomatologie [Clinique Bretéché, Nantes], Clinique Bretéché [Nantes], Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'anesthésie et réanimation chirurgicale [Nantes], Hôtel-Dieu-Centre hospitalier universitaire de Nantes (CHU Nantes), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)
Subjects: 2019-20 coronavirus outbreak, [SDV.MHEP.AHA] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Consensus, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Pneumonia, Viral, 03 medical and health sciences, Betacoronavirus, 0302 clinical medicine, Pandemic, [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Medicine, Humans, Facial Injuries, Pandemics, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, biology, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, business.industry, SARS-CoV-2, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, COVID-19, 030208 emergency & critical care medicine, 030206 dentistry, biology.organism_classification, Virology, Otorhinolaryngology, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Surgery, Oral Surgery, business, Coronavirus Infections
Abstract: International audience; no abstract
Published: 2020

5. Correcting the side effects of ADC filtering in MR image reconstruction

Author: Maximilian März, Carole Lazarus, Pierre Weiss, Service NEUROSPIN (NEUROSPIN), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Weiss, Pierre
Subjects: Statistics and Probability, Scanner, [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, Computer science, Image quality, [INFO.INFO-IM] Computer Science [cs]/Medical Imaging, ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION, 02 engineering and technology, Sampling (signal processing), [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, 0202 electrical engineering, electronic engineering, information engineering, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Point (geometry), Limit (mathematics), [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, Applied Mathematics, Converters, Condensed Matter Physics, Nonlinear system, Amplitude, Modeling and Simulation, 020201 artificial intelligence & image processing, Geometry and Topology, Computer Vision and Pattern Recognition, Algorithm, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Abstract: This work investigates the role of the filters implemented on analog-to-digital converters for the reconstruction of magnetic resonance images. We analyze the effects of these filters both from a theoretical and an experimental point of view and demonstrate how it may lead to severe degradation of the reconstructed images when the distance between consecutive samples is larger than Shannon’s limit. Based on these findings, we propose a mathematical model and a numerical algorithm that allow to mitigate such filtering effects both for linear and nonlinear reconstructions. Experiments on simulated and real data on a 7 Tesla scanner show that the proposed ideas allow to significantly improve the overall image quality. These findings are particularly relevant for high resolution imaging and for recent sampling schemes saturating the maximum gradient amplitude. They also open new challenges in sampling theory.
Published: 2020

6. Degenerative lumbar disc disease: in vivo data support the rationale for the selection of appropriate animal models

Author: Fusellier, M, Clouet, J, Gauthier, O, Tryfonidou, M, Le Visage, C, Guicheux, J, Orthopedie en neurochirurgie, dCSCA RMSC-1, Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Unité de Biotechnologie, Biocatalyse et Biorégulation (U3B), Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Jehan, Frederic, Orthopedie en neurochirurgie, and dCSCA RMSC-1
Subjects: [SDV.MHEP.AHA] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], lcsh:Diseases of the musculoskeletal system, 0206 medical engineering, lcsh:Surgery, review, degeneration, 02 engineering and technology, Degeneration (medical), Intervertebral Disc Degeneration, Animal model, In vivo, Outcome Assessment, Health Care, medicine, [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Animals, Humans, Disease process, Animal species, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Lumbar Vertebrae, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, business.industry, animal model, nucleus pulposus, disease model, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Intervertebral disc, lcsh:RD1-811, medicine.disease, 020601 biomedical engineering, Biomechanical Phenomena, Disease Models, Animal, medicine.anatomical_structure, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Disc degeneration, intervertebral disc, lcsh:RC925-935, business, Neuroscience, Lumbar disc disease
Abstract: Since low-back pain is increasing in ageing populations, current research efforts are focused on obtaining a better understanding of the pathophysiology of intervertebral disc degeneration and on developing new therapeutic strategies. This requires adequate and clinically relevant models of the disease process. Ex vivo models can provide insights into isolated aspects of the degenerative/regenerative processes involved; although, ultimately, in vivo models are needed for preclinical translational studies. Such models have been developed in numerous animal species with significant variations in size and disc physiology and their number is considerable. Importantly, the choice of the model has to be tailored to the aim of the study. Given the number of available options, it is important to have a good understanding of the various models of disc degeneration and to be fully aware of their advantages and limitations. After comparing the anatomy and histology of intervertebral discs in animals and humans, the present study provides an overview of the different models of in vivo disc degeneration. It also provides a comprehensive guide with suggested criteria to select the most appropriate animal model in a question-driven manner.
Published: 2020

7. Should a neck dissection be performed on patients with cN0 adenoid cystic carcinoma? A REFCOR propensity score matching study

Author: Sylvain Morinière, Christian Righini, Sarah Atallah, Franck Jegoux, Nicolas Fakhry, Esteban Brenet, Bertrand Baujat, Pierre Philouze, Haitham Mirghani, Patrice Gallet, Antoine Moya-Plana, Benjamin Verillaud, Ludovic de Gabory, Philippe Schultz, Vianney Bastit, Gilles Poissonnet, Chloé Bertolus, Nicolas Bon-Mardion, Olivier Malard, Sébastien Vergez, Jérémie Bettoni, Xavier Dufour, Olivier Mauvais, Maria Lesnik, Diane Evrard, Pierre Ransy, Julian Biau, Service d'ORL pédiatrique et Chirurgie Cervico-faciale [CHU Trousseau], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université-Sorbonne Université, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Lutte contre le Cancer Antoine Lacassagne [Nice] (UNICANCER/CAL), UNICANCER-Université Côte d'Azur (UCA), ORL et Chirurgie cervico-faciale pédiatrique - [Hôpitaux Timone et Nord - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE)- Hôpital Nord [CHU - APHM], Département de chirurgie maxillofaciale et stomatologie [CHU d'Amiens-Picardie], CHU Amiens-Picardie, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital of Liege, Belgium, Institut de mécanique des fluides de Toulouse (IMFT), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Hôpital Pellegrin, CHU Bordeaux [Bordeaux]-Groupe hospitalier Pellegrin, Service d’Otorhino-laryngologie et de chirurgie cervicofaciale [CHU Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [APHP], Service d'ORL et de Chirurgie cervico-faciale - Hôpital Lyon-Sud, INSERM Unit 708 Hôpital La Salpêtrière, Université Pierre et Marie Curie-Paris, Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et nanosciences d'Alsace, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA), Service ORL et chirurgie cervico-faciale [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau, UNIROUEN - UFR Santé (UNIROUEN UFR Santé), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), INSERM U823, équipe 5 (cibles diagnostiques ou thérapeutiques et vectorisation de drogues dans le cancer du poumon), Service d'ORL et de chirurgie cervicale, CHU Grenoble-CHU Grenoble-Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-UF cancérologie biologique et biothérapie, CHU Grenoble, LPP - Laboratoire de Phonétique et Phonologie - UMR 7018 (LPP), Université Sorbonne Nouvelle - Paris 3-Centre National de la Recherche Scientifique (CNRS), Centre hospitalier universitaire de Nantes (CHU Nantes), Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe de Recherche sur le Handicap Ventilatoire (GRHV), Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-CHU Rouen, Normandie Université (NU), Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital of Reims, Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire Parole et Langage (LPL), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), CHU Tenon [AP-HP], Université Pierre et Marie Curie - Paris 6 (UPMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hôpital Bretonneau-Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-Université Joseph Fourier - Grenoble 1 (UJF)-UF cancérologie biologique et biothérapie, Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), and Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, 0301 basic medicine, Cancer Research, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Gastroenterology, 0302 clinical medicine, Prospective Studies, Prospective cohort study, Lymph node, ComputingMilieux_MISCELLANEOUS, Aged, 80 and over, Prophylactic Surgical Procedures, Middle Aged, Prognosis, Salivary Gland Neoplasms, Carcinoma, Adenoid Cystic, Treatment Outcome, medicine.anatomical_structure, Oncology, Head and Neck Neoplasms, Lymphatic Metastasis, 030220 oncology & carcinogenesis, Female, France, Adult, medicine.medical_specialty, Adolescent, Adenoid cystic carcinoma, Matched-Pair Analysis, Neck dissection, Young Adult, 03 medical and health sciences, Internal medicine, medicine, Humans, Propensity Score, Survival analysis, Aged, Neoplasm Staging, Salivary gland cancer, business.industry, medicine.disease, Survival Analysis, REFCOR, Confidence interval, 030104 developmental biology, Propensity score matching, Surgery, Lymph Nodes, business
Abstract: Background Patterns of nodal involvement in adenoid cystic carcinoma (ACC) of the head and neck have not been sufficiently assessed to guide a decision of prophylactic neck dissection (ND). The objective of this study is to analyse the influence of ND on event-free survival (EFS) for patients with cN0 ACC. Patients and methods A multicentre prospective study was conducted between 2009 and 2018. Patients presenting cN0 non-metastatic ACC on any site, and who received surgery on the tumour, were included. EFS was the main judgement criterion. A comparative survival analysis between the groups that received a ND versus those that did not was performed, using a propensity score. Analyses were carried out using the R software. Results Between 2009 and 2018, 322 patients with cN0 ACC were included, out of which 58% were female. The average age was 53 years. Tumours were in minor salivary glands in 58% of cases, and 52% had T3/T4 stages. ND was performed on 46% of patients. Out of them, seven had histological lymph node invasion, out of which six had tumour infiltration in the mucosa of oral cavity. After propensity score, the median EFS for N0 patients with ND was 72 months (95% Confidence Interval (CI) [48–81]), compared to 73 months (95% CI [52–85]) for patients without ND (HR = 1.33; 95% CI [0.82–2.16]; p = 0.2). Conclusion ND of cN0 patients does not provide any benefit on EFS, which suggests that its application on such patients is not necessary.
Published: 2020

8. Good clinical practice recommendations for the use of PET/CT in oncology

Author: Arnaud Philip, Olivier Casasnovas, G. Quere, Caroline Bodet-Milin, Emmanuel Itti, David Groheux, Caroline Rousseau, Antoine Girard, Désirée Deandreis, Ronan Abgral, Philippe Moreau, Corinne Balleyguier, Pierre-Yves Salaun, Jean-Louis Alberini, Myriam Wartski, Solène Querellou-Lefranc, Florent Cachin, Isabelle Brenot-Rossi, Romain Coriat, Françoise Kraeber-Bodéré, David Taïeb, Antoine Tabarin, Jean-François Grellier, Olivier Malard, Alain Luciani, Elif Hindié, COLO, Mouniati, CHRU Brest - Service de médecine nucléaire (CHU - BREST - Med Nucléaire), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), University Hospital of Brest, Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de Gastro-entérologie [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Hôpital Haut-Lévêque [CHU Bordeaux], CHU Bordeaux [Bordeaux], Département de Médecine Nucléaire [AP-HM], Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE)- Hôpital Nord [CHU - APHM], Département d'endocrinologie - Bordeaux 2, Université Bordeaux Segalen - Bordeaux 2, Laboratoire des signaux et systèmes (L2S), Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Foch Hospital, Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Hopital Saint-Louis [AP-HP] (AP-HP), University Hospital of Nantes, Médecine nucléaire, Département d'imagerie médicale [Gustave Roussy], Institut Gustave Roussy (IGR)-Institut Gustave Roussy (IGR), Institut Gustave Roussy (IGR), Laboratoire d'Imagerie Moléculaire Expérimentale (LIME), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service de médecine nucléaire [Créteil], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital du Bocage, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre hospitalier universitaire de Nantes (CHU Nantes), Service de Radiologie Adultes, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Equipe IFTIM [ImViA - EA7535], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Imagerie et Vision Artificielle [Dijon] (ImViA), Université de Bourgogne (UB)-Université de Bourgogne (UB), and Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Oncology, Positron emission tomography, medicine.medical_specialty, Referral, [SDV]Life Sciences [q-bio], Computed tomography, [SDV.CAN]Life Sciences [q-bio]/Cancer, Methodological approval, Medical Oncology, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, [SDV.CAN] Life Sciences [q-bio]/Cancer, Neoplasms, Positron Emission Tomography Computed Tomography, Internal medicine, Humans, Medicine, Radiology, Nuclear Medicine and imaging, ComputingMilieux_MISCELLANEOUS, PET-CT, medicine.diagnostic_test, business.industry, General Medicine, Focus group, 3. Good health, 030220 oncology & carcinogenesis, Good clinical practice, Clinical value, Nuclear Medicine, business
Abstract: Positron emission tomography/computed tomography (PET/CT) is a nuclear medicine functional imaging technique with proven clinical value in oncology. PET/CT indications are continually evolving with fresh advances made through research. French practice on the use of PET in oncology was framed in recommendations based on Standards-Options-Recommendations methodology and coordinated by the French federation of Comprehensive Cancer Centres (FNLCC). The recommendations were originally issued in 2002 followed by an update in 2003, but since then, a huge number of scientific papers have been published and new tracers have been licenced for market release. The aim of this work is to bring the 2003 version recommendations up to date. For this purpose, a focus group was set up in collaboration with the French Society for Nuclear Medicine (SFMN) to work on developing good clinical practice recommendations. These good clinical practice recommendations have been awarded joint French National Heath Authority (HAS) and French Cancer Institute (INCa) label status-the stamp of methodological approval. The present document is the outcome of comprehensive literature review and rigorous appraisal by a panel of experts, organ specialists, clinical oncologists, surgeons and imaging specialists. These data were also used for the EANM referral guidelines.
Published: 2020

9. IL-36γ Is a Pivotal Inflammatory Player in Periodontitis-Associated Bone Loss

Author: Cloitre, Alexandra, Halgand, Boris, Sourice, Sophie, Caillon, Jocelyne, Huck, Olivier, Bugueno, Isaac, Batool, Fareeha, Guicheux, Jerome, Geoffroy, Valérie, Lesclous, Philippe, Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre hospitalier universitaire de Nantes (CHU Nantes), Nanomédecine Régénérative (NanoRegMed), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Jehan, Frederic
Subjects: Inflammation, Male, lcsh:R, Alveolar Bone Loss, lcsh:Medicine, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Article, Cell Line, Osteoimmunology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Bacteroidaceae Infections, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Humans, Female, lcsh:Q, Periodontitis, lcsh:Science, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, Porphyromonas gingivalis, Interleukin-1
Abstract: International audience; Periodontitis is a prevalent chronic inflammatory disease due to the host response (IL-1β, IL-6, TNF-α and IL-17A) to oral bacteria such as Porphyromonas gingivalis. The newer members of the IL-1 family, IL-36s (IL-36α/IL-36β/IL-36γ/IL-36Ra/IL-38) are known to be involved in host defense against P. gingivalis in oral epithelial cells (OECs) and are considered as key inflammatory mediators in chronic diseases. The aim of this study was to investigate the potential role of IL-36s in periodontitis. We showed here that IL-36γ mRNA gingival expression is higher in periodontitis patients, whereas IL-36β and IL-36Ra mRNA expression are lower compared to healthy controls. Interestingly, the elevated IL-36γ expression in patients is positively correlated with the RANKL/OPG ratio, an index of bone resorption. In vitro, IL-36γ expression was induced through TLR2 activation in primary OECs infected with P. gingivalis but not in gingival fibroblasts, the most widespread cell type in gingival connective tissue. In OECs, recombinant IL-36γ enhanced the expression of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-36γ), of TLR2 and importantly, the RANKL/OPG ratio. These findings suggest that IL-36γ could be a pivotal inflammatory player in periodontitis by perpetuating gingival inflammation and its associated alveolar bone resorption and could be a relevant therapeutic target.
Published: 2019

10. Calcium phosphate powder synthesis by out-of-phase pulsed sonoelectrochemistry

Author: T. Rouillon, Anne-Lise Daltin, Pierre Millet, Jean-Paul Chopart, S Beaufils, Laboratoire d'Ingéniérie et Science des Matériaux - EA 4695 (LISM), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Thermique, Mécanique, Matériaux (ITheMM), Université de Reims Champagne-Ardenne (URCA), and CCSD, Accord Elsevier
Subjects: Materials science, Acoustics and Ultrasonics, chemistry.chemical_element, 02 engineering and technology, Calcium, [SPI.MAT] Engineering Sciences [physics]/Materials, 010402 general chemistry, 01 natural sciences, Calcium nitrate, [SPI.MAT]Engineering Sciences [physics]/Materials, Inorganic Chemistry, chemistry.chemical_compound, [CHIM] Chemical Sciences, Chemical Engineering (miscellaneous), Environmental Chemistry, [CHIM]Chemical Sciences, Radiology, Nuclear Medicine and imaging, Fourier transform infrared spectroscopy, Spectroscopy, ComputingMilieux_MISCELLANEOUS, Aqueous solution, Organic Chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Transmission electron microscopy, Nanorod, 0210 nano-technology, Powder diffraction, Nuclear chemistry
Abstract: International audience; High aspect ratio calcium phosphate (CaP) nanorods were achieved by out-of-phase pulsed sonoelectrodeposition from electrolytic aqueous bath composed of calcium nitrate, ammonium dihydrogenophosphate and surfactant at pH of 4.9. The nature of CaP phases was determined by powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR) and energy dispersive X-ray spectroscopy (EDX). The results reveal the predominantly presence of calcium deficient hydroxyapatite (CDHA). The transmission electron microscopy (TEM) analyzes highlighted that the nanorods are polycristalline and have an aspect ratio up to 30.
Published: 2019

11. Clinical management of an atypical dental invagination

Author: Badran, Zahi, Lopez-Cazaux, Serena, Crauste, Eleonore, Bray, Estelle, Soueidan, Assem, Armengol, Valérie, Di Donato, N., Isidor, B., Lopez Cazaux, S., Le Caignec, C., Klink, B., Kraus, C., Schrock, E., Hackmann, K., Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Service d’Odontologie Conservatrice et Pédiatrique [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre de compétences Malformations orales et dentaires rares [CHU Nantes], Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Dentisterie Conservatrice et d'Endodontie [Hôtel Dieu, Nantes], Hôtel-Dieu de Nantes, Service de génétique médicale - Unité de génétique clinique [Nantes], Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Jehan, Frederic, Regenerative Medicine and Skeleton (RMeS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)
Subjects: [SDV]Life Sciences [q-bio], Dentistry, Case Report, TOOTH MALFORMATION, Oral hygiene, 030207 dermatology & venereal diseases, 03 medical and health sciences, Dens invaginatus, 0302 clinical medicine, Incisor, stomatognathic system, medicine, General Dentistry, Attachment loss, ComputingMilieux_MISCELLANEOUS, Permanent teeth, Orthodontics, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, business.industry, dental invagination, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Invagination, 030206 dentistry, medicine.disease, stomatognathic diseases, malformation, medicine.anatomical_structure, Clinical attachment loss, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Etiology, business
Abstract: Dental invagination (DI) is a tooth malformation that usually affects permanent teeth. Its precise etiology is still controversial and represents a clinical challenge as it can favor the development of carious lesion or periodontal inflammation. This paper presents a case of a 23-year-old Caucasian male, where an atypical buccal DI could not be completely diagnosed in the dens invaginatus category. Furthermore, other differential diagnoses could not be confirmed. The dental malformation was seen on a permanent maxillary first incisor and was associated with periodontal inflammation and attachment loss. Successful clinical management of this case consisted of surgical restorative treatment and regular follow-up, accompanied by thorough oral hygiene procedures.
Published: 2019

12. Iterative Discretization of Optimization Problems Related to Superresolution

Author: Pierre Weiss, Axel Flinth, Technische Universität Berlin (TU), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), and Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Optimization problem, Discretization, 020206 networking & telecommunications, 010103 numerical & computational mathematics, 02 engineering and technology, Discretization algorithm, 01 natural sciences, Superresolution, Omega, Norm (mathematics), Bounded function, 0202 electrical engineering, electronic engineering, information engineering, Applied mathematics, [MATH.MATH-OC]Mathematics [math]/Optimization and Control [math.OC], 0101 mathematics, Linear convergence rate, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Mathematics
Abstract: We study an iterative discretization algorithm for solving optimization problems regularized by the total variation norm over the space $\mathcal{M}\left( \Omega \right)$ of Radon measures on a bounded subset Ω of ${\mathbb{R}^d}.$ Our main motivation to study this problem is the recovery of sparse atomic measures from linear measurements. Under reasonable regularity conditions, we arrive at a linear convergence rate guarantee.
Published: 2019

13. Beta tricalcium phosphate ceramic triggers fast and robust bone formation by human mesenchymal stem cells

Author: Monica Scaranari, Chiara Gentili, Ranieri Cancedda, Barbara Canciani, Rui C. Pereira, Roberto Benelli, Guy Daculsi, Jehan, Frederic, Laboratory of Regenerative Medicine [Genoa, Italy] (DIMES), Department of Experimental Medicine - DIMES [Genoa, Italy] (DIMES), Università degli studi di Genova = University of Genoa (UniGe)-Università degli studi di Genova = University of Genoa (UniGe), Laboratory of Immunology [Genoa, Italy], IRCCS Azienda Ospedaliera Universitaria Integrata San Martino (IRCCS AOU San Martino), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre of Excellence for Biomedical Research [Genova, Italy] (CEBR), and University of Genova [Genova, Italy]
Subjects: Calcium Phosphates, Ceramics, Mature Bone, Angiogenesis, 0206 medical engineering, Biomedical Engineering, Bone Morphogenetic Protein 2, Mice, Nude, Neovascularization, Physiologic, regenerative medicine, Medicine (miscellaneous), chemistry.chemical_element, 02 engineering and technology, Bone healing, Calcium, calcium phosphate, osteogenesis, Biomaterials, 03 medical and health sciences, Nude mouse, vascularization, Human Umbilical Vein Endothelial Cells, Animals, Humans, calcium phosphate, osteogenesis, regenerative medicine, vascularization, ComputingMilieux_MISCELLANEOUS, Cell Proliferation, 030304 developmental biology, 0303 health sciences, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Tissue Engineering, biology, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Mesenchymal stem cell, Cell Differentiation, Mesenchymal Stem Cells, biology.organism_classification, 020601 biomedical engineering, Cell biology, Endothelial stem cell, Durapatite, chemistry, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Stem cell, Receptors, Calcium-Sensing, Signal Transduction
Abstract: Due to their osteoconductive and inductive properties, a variety of calcium phosphate (CaP) scaffolds are commonly used in orthopaedics as graft material to heal bone defects. In this study, we have used two CaP scaffolds with different hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) ratios (MBCP®; 60/40 and MBCP+ ®; 20/80) to investigate their intrinsic capacity to favour human bone marrow stem cells (hBMSCs) osteogenic differentiation capacity. We report that MBCP+ ® showed in in vitro culture model a higher rate of calcium ion release in comparison with MBCP®. In two defined coculture systems, the hBMSC seeded onto MBCP+ ® presented an increased amount of VEGF secretion, resulting in an enhanced endothelial cell proliferation and capillary formation compared with hBMSC seeded onto MBCP®. When both ceramics combined with hBMSC were implanted in a nude mouse model, we observed a faster osteogenic differentiation and enhancement mature bone deposition sustained by the presence of a vast host vasculature within the MBCP+ ® ceramics. Bone formation was observed in samples highly positive to the activation of calcium sensing receptor protein (CaSr) on the surface of seeded hBMSC that also shown higher BMP-2 protein expression. With these data we provide valuable insights in the possible mechanisms of ossification and angiogenesis by hBMSC that we believe to be primed by calcium ions released from CaP scaffolds. Evidences could lead to an optimization of ceramic scaffolds to prime bone repair.
Published: 2019

14. Jaw osteosarcoma models in mice: first description

Author: Bertin, Helios, Guilho, Romain, Brion, Régis, Amiaud, Jérôme, Battaglia, Séverine, Moreau, Anne, Brouchet-Gomez, Anne, Longis, Julie, Piot, Benoît, Heymann, Dominique, Corre, Pierre, Rédini, Françoise, Sarcomes osseux et remodelage des tissus calcifiés - Phy-Os [Nantes - INSERM U1238] (Phy-Os), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Département de Stomatologie et chirurgie maxillo-faciale [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Faculty of Population Health Sciences, UCL Institute of Child Health, Laboratoire d'anatomie et cytologie pathologiques [CHU Nantes], Institut Universitaire du Cancer de Toulouse - Oncopole (IUCT Oncopole - UMR 1037), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Hétérogénéité Tumorale et Médecine de Précision, Institut de Cancérologie de l'Ouest [Angers/Nantes] (UNICANCER/ICO), UNICANCER-UNICANCER, Service d'Histologie-Embryologie [CHU Nantes], Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), This research received a financial support from 'Fondation pour la Recherche Médicale' (DEA20140629835), and from 'Fondation des Gueules Cassées' (2018–51)., Jehan, Frederic, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Lung Neoplasms, lcsh:Medicine, Bone resorption, Mandible, Mice, SCID, Environment, Models, Cell Line, Tumor, Animals, Humans, Cell Proliferation, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Osteosarcoma, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Research, lcsh:R, Sarcoma, X-Ray Microtomography, Jaw Neoplasms, Xenograft Model Antitumor Assays, Tumor Burden, Mice, Inbred C57BL, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Animal experimentation, Female
Abstract: Background Osteosarcoma (OS) is the most common cancer of bone. Jaw osteosarcoma (JOS) is rare and it differs from other OS in terms of the time of occurrence (two decades later) and better survival. The aim of our work was to develop and characterize specific mouse models of JOS. Methods Syngenic and xenogenic models of JOS were developed in mice using mouse (MOS-J) and human (HOS1544) osteosarcoma cell lines, respectively. An orthotopic patient-derived xenograft model (PDX) was also developed from a mandibular biopsy. These models were characterized at the histological and micro-CT imaging levels, as well as in terms of tumor growth and metastatic spread. Results Homogeneous tumor growth was observed in both the HOS1544 and the MOS-J JOS models by injection of 0.25 × 106 and 0.50 × 106 tumor cells, respectively, at perimandibular sites. Histological characterization of the tumors revealed features consistent with high grade conventional osteosarcoma, and the micro-CT analysis revealed both osteogenic and osteolytic lesions. Early metastasis was encountered at day 14 in the xenogenic model, while there were no metastatic lesions in the syngenic model and in the PDX models. Conclusion We describe the first animal model of JOS and its potential use for therapeutic applications. This model needs to be compared with the usual long-bone osteosarcoma models to investigate potential differences in the bone microenvironment. Electronic supplementary material The online version of this article (10.1186/s12967-019-1807-5) contains supplementary material, which is available to authorized users.
Published: 2018

15. Ageing in the musculoskeletal system

Author: Roberts, S, Colombier, P, Sowman, A, Mennan, C, Rolfing, J, Guicheux, J, Edwards, J, Spinal Studies and ISTM [Oswestry, UK], Keele University [Staffordshire, U.K.]-Robert Jones and Agnes Hunt Orthopaedic Hospital [Oswestry, UK], Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences [Oxford, UK] (Botnar Research Centre), University of Oxford [Oxford], Orthopaedic Research Laboratory [Aarhus, Denmark], Departments of Orthopaedics [Aarhus, Denmark], Aarhus University Hospital-Aarhus University Hospital, Jehan, Frederic, and University of Oxford
Subjects: Cartilage, Articular, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Aging, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Articles, R1, Bone and Bones, Chondrocytes, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Humans, Intervertebral Disc, Musculoskeletal System, Cellular Senescence
Abstract: International audience; The extent of ageing in the musculoskeletal system during the life course affects the quality and length of life. Loss of bone, degraded articular cartilage, and degenerate, narrowed intervertebral discs are primary features of an ageing skeleton, and together they contribute to pain and loss of mobility. This review covers the cellular constituents that make up some key components of the musculoskeletal system and summarizes discussion from the 2015 Aarhus Regenerative Orthopaedic Symposium (AROS) (Regeneration in the Ageing Population) about how each particular cell type alters within the ageing skeletal microenvironment.
Published: 2016

16. Patients’ exposure to PVC plasticizers from ECMO circuits

Author: Lise Bernard, Teuta Eljezi, Jean-Michel Constantin, Bruno Pereira, Lionel Camilleri, Sophie Kauffmann, Pierre-Gabriel Pinta, Valérie Sautou, Gaël Grimandi, Charlotte Fernandez-Canal, Bernard Cosserant, Virginie Larbre, CHU Clermont-Ferrand, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Chimie de Clermont-Ferrand (ICCF), SIGMA Clermont (SIGMA Clermont)-Institut de Chimie du CNRS (INC)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Service de chirurgie cardiaque, and service de Biostatistiques, DRCI
Subjects: endocrine system, plasticizers, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Biomedical Engineering, 030204 cardiovascular system & hematology, 010501 environmental sciences, migration, 01 natural sciences, Cell Line, medical devices, Mice, 03 medical and health sciences, 0302 clinical medicine, Diethylhexyl Phthalate, Extracorporeal membrane oxygenation, medicine, Animals, Humans, Polyvinyl Chloride, 0105 earth and related environmental sciences, Chromatography, Cell Death, Chemistry, Plasticizer, General Medicine, surgical procedures, operative, cytotoxicity, Surgery, Rheology
Abstract: International audience; Background: ECMO is a therapeutic act with a high risk of exposure to diethylhexylphthalate (DEHP), plasticizer from PVC tubings. The replacement of this plasticizer with alternative compounds is recommended but the risks associated with the use of new plasticizers have not been evaluated in ECMO situations.Methods: Ex vivo ECMO models were performed with different flow rates over 6 days to evaluate the migration of plasticizers and their potential toxic risk for patient. The release of plasticizers during ECMO was measured and compared to reference value (derived no effect level, DNEL) and to cytotoxic concentration carried out with MTT test.Results: Trioctyltrimellitate (TOTM), main plasticizer present in circuit (44% w/w), is weakly released during ECMO. Concentrations are not cytotoxic and exposure doses are lower than DNEL. In contrast, DEHP doses are higher than the DNEL despite a lower presence of DEHP in the circuit (0.2%). We have shown that DEHP is not coming from the circuit but from the priming bag. Replacing this bag with a multilayer one avoids the exposure to DEHP.Conclusion: Our study shows that circuits made of PVC plasticized with TOTM against DEHP improves the safety of ECMO.
Published: 2018

17. B cell subset distribution is altered in patients with severe periodontitis

Author: Pierre Pochard, Quentin Simon, Sylvie Boisramé, Julien Demoersman, Camille Framery, Assem Soueidan, Jacques-Olivier Pers, LabEX IGO Immunothérapie Grand Ouest, Lymphocyte B et Auto-immunité (LBAI), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), CHRU Brest - Service d'Odontologie (CHU - BREST - Odontologie), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Periodontology, Nantes, Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Nantes Université (Nantes Univ), Lymphocytes B, Autoimmunité et Immunothérapies (LBAI), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-LabEX IGO Immunothérapie Grand Ouest, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Institut Brestois Santé Agro Matière (IBSAM), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Michel, Geneviève, and Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE)
Subjects: 0301 basic medicine, Male, B Cells, Physiology, [SDV]Life Sciences [q-bio], lcsh:Medicine, Pilot Projects, Severity of Illness Index, White Blood Cells, 0302 clinical medicine, Oral Diseases, Animal Cells, Medicine and Health Sciences, Medicine, lcsh:Science, CD20, Multidisciplinary, medicine.diagnostic_test, biology, Flow Cytometry, 3. Good health, Body Fluids, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, Blood, RANKL, Female, Cellular Types, Anatomy, Research Article, Adult, Immune Cells, Inflammatory Diseases, Immunology, Oral Medicine, Plasma Cells, B-Lymphocyte Subsets, Context (language use), Severe periodontitis, Flow cytometry, Autoimmune Diseases, 03 medical and health sciences, Humans, Antibody-Producing Cells, Periodontitis, B cell, Periodontal Diseases, Blood Cells, business.industry, lcsh:R, Biology and Life Sciences, 030206 dentistry, Cell Biology, medicine.disease, Memory B cells, B-1 cell, 030104 developmental biology, biology.protein, lcsh:Q, Clinical Immunology, Clinical Medicine, business
Abstract: International audience; Several studies have recently highlighted the implication of B cells in physiopathogenesis of periodontal disease by showing that a B cell deficiency leads to improved periodontal parameters. However, the detailed profiles of circulating B cell subsets have not yet been investigated in patients with severe periodontitis (SP). We hypothesised that an abnormal distribution of B cell subsets could be detected in the blood of patients with severe periodontal lesions, as already reported for patients with chronic inflammatory diseases as systemic autoimmune diseases. Fifteen subjects with SP and 13 subjects without periodontitis, according to the definition proposed by the CDC periodontal disease surveillance work group, were enrolled in this pilot observational study. Two flow cytometry panels were designed to analyse the circulating B and B1 cell subset distribution in association with the RANKL expression. A significantly higher percentage of CD27+ memory B cells was observed in patients with SP. Among these CD27+ B cells, the proportion of the switched memory subset was significantly higher. At the same time, human B1 cells, which were previously associated with a regulatory function (CD20+CD69-CD43+CD27+CD11b+), decreased in SP patients. The RANKL expression increased in every B cell subset from the SP patients and was significantly greater in activated B cells than in the subjects without periodontitis. These preliminary results demonstrate the altered distribution of B cells in the context of severe periodontitis. Further investigations with a larger cohort of patients can elucidate if the analysis of the B cell compartment distribution can reflect the periodontal disease activity and be a reliable marker for its prognosis (clinical trial registration number: NCT02833285, B cell functions in periodontitis).
Published: 2018

18. Outcomes of functional treatment versus open reduction and internal fixation of condylar mandibular fracture with articular impact: A retrospective study of 83 adults

Author: M. Poisson, Pierre Corre, A. Pare, F-L Merlet, Fanny Grimaud, Richard Pace, J-M Mercier, Service de chirurgie maxillo-faciale et stomatologie [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de chirurgie maxillo-faciale [CHRU Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Jehan, Frederic, Service de Chirurgie maxillo-faciale et stomatologie, Hôpital Trousseau, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR26-Université de Nantes (UN), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)
Subjects: Adult, Male, Mandibular fracture, medicine.medical_treatment, Dentistry, Condyle, 03 medical and health sciences, Fracture Fixation, Internal, 0302 clinical medicine, stomatognathic system, Mandibular Fractures, medicine, Internal fixation, Humans, Functional treatment, Range of Motion, Articular, Child, Reduction (orthopedic surgery), Retrospective Studies, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, business.industry, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Mandible, Retrospective cohort study, 030206 dentistry, musculoskeletal system, medicine.disease, 3. Good health, Functional Treatment, Treatment Outcome, Otorhinolaryngology, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, 030220 oncology & carcinogenesis, Radiological weapon, Condylar process fracture, Surgery, Oral Surgery, business, ORIF
Abstract: INTRODUCTION:The treatment of fractures in the mandibular condylar process remains controversial. The aim of this study was to assess the outcomes of isolated functional treatment versus open reduction and internal fixation (ORIF) of mandibular condylar fracture with articular impact based on clinical and radiological criteria.MATERIALS AND METHODS:Eighty-three patients with a mandibular condylar fracture with articular impact were included in this retrospective study. They were divided according to Loukota, Spiessl and Schroll, Mercier and Rasse, Neff, and Hlawitschka classifications. Two groups were created: operated patients (operated) and non-operated patients (non-operated). Occlusal and functional features were evaluated using clinical measurements at 1, 3, 6, and 12 months after the treatment as well as radiological measurements performed preoperatively, 6 weeks later, and at the end of the follow-up.RESULTS:A male predominance was observed in the data (69.9%, P0.05). There was no difference concerning temporo-mandibular joint dysfunctions between operated and non-operated patients. For unilateral fractures, the loss of height of the ramus was significantly higher in operated patients initially compared to "non-operated" group (P=0.0137). After surgical correction, there was no difference between the two sides of mandible. At the end of the follow-up, the there was no difference between operated and non-operated ramus (P=0.1304 and 0.6420).CONCLUSION:The present study showed that a properly followed isolated functional treatment provided similar clinical results to ORIF for mandibular condylar fractures with articular impact. Surgical treatment should be preferred when the loss of height of the ramus is severe to restore the ramus height since adult condylar remodeling is less efficient than in children., HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. mandibular fracture with articular impact: a retrospective study of 83 adults Merlet Fanny-Laure (1), Grimaud Fanny (1), Pace Richard (2), Mercier Jacques-Marie(1), Paré Arnaud(3), Corre Pierre(1). 1) Service de Chirurgie maxillo-faciale et stomatologie, CHU NANTES
Published: 2018

19. Fibrosis and Hypoxia-Inducible Factor-1α–Dependent Tumors of the Soft Tissue on Loss of Von Hippel-Lindau in Mesenchymal Progenitors

Author: Tremika L.S. Wilson, Christophe Merceron, George E. Sandusky, Ernestina Schipani, Rita Gerard-O'Riley, Amato J. Giaccia, Laura Mangiavini, Karen M. Lyons, Jérôme Abadie, Richa Khatri, Elisa Araldi, Department of Orthopaedic Surgery [Ann Arbor, MI, USA] (Department of Medicine), University of Michigan [Ann Arbor], University of Michigan System-University of Michigan System, Division of Endocrinology [Ann Arbor, MI, USA], Department of Orthopaedic and Traumatology [Milan, Italy], Università degli Studi di Milano-Bicocca [Milano] (UNIMIB), Endocrine Unit [Boston, Massachusetts], Massachusetts General Hospital [Boston], Groupe Skeletal Tissue Engineering and Physiopathology (Groupe STEP - Inserm U791 - LIOAD), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR Odontologie, Université de Nantes (UN), PRES Université Nantes Angers Le Mans (UNAM), Department of Medicine, and the Department of Pathology [Indianapolis, IN, USA] (School of Medicine), Indiana University System, Animaux modèles pour la recherche en oncologie comparée (AMaROC), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Division of Radiation and Cancer Biology [Stanford, California, USA] (Center for Clinical Sciences Research), Department of Radiation Oncology [Stanford], Stanford Medicine, Stanford University-Stanford University-Stanford Medicine, Stanford University-Stanford University, Jehan, Frederic, Università degli Studi di Milano-Bicocca = University of Milano-Bicocca (UNIMIB), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Stanford University-Department of Radiation Oncology [Stanford], and Stanford University
Subjects: Pathology, medicine.medical_specialty, Tumor suppressor gene, Connective tissue, Mice, Transgenic, Soft Tissue Neoplasms, Inbred C57BL, alpha Subunit, Medical and Health Sciences, Transgenic, Article, Pathology and Forensic Medicine, Mice, 03 medical and health sciences, 0302 clinical medicine, Fibrosis, Basic Helix-Loop-Helix Transcription Factors, medicine, Animals, Humans, 030304 developmental biology, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, 0303 health sciences, Mesenchymal Stromal Cells, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, biology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Synovial Membrane, Mesenchymal stem cell, EPAS1, Mesenchymal Stem Cells, Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, 3. Good health, Ubiquitin ligase, Mice, Inbred C57BL, medicine.anatomical_structure, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Hypoxia-inducible factors, Von Hippel-Lindau Tumor Suppressor Protein, 030220 oncology & carcinogenesis, biology.protein, Cancer research, Hypoxia-Inducible Factor 1, Synovial membrane, Signal Transduction
Abstract: International audience; The hypoxia-inducible factor (Hif)-1α (Hif-1α) and Hif-2α (Epas1) have a critical role in both normal development and cancer. von Hippel Lindau (Vhl) protein, encoded by a tumor suppressor gene, is an E3 ubiquitin ligase that targets Hif-1α and Epas1 to the proteasome for degradation. To better understand the role of Vhl in the biology of mesenchymal cells, we analyzed mutant mice lacking Vhl in mesenchymal progenitors that give rise to the soft tissues that form and surround synovial joints. Loss of Vhl in mesenchymal progenitors of the limb bud caused severe fibrosis of the synovial joints and formation of aggressive masses with histologic features of mesenchymal tumors. Hif-1α and its downstream target connective tissue growth factor were necessary for the development of these tumors, which conversely still developed in the absence of Epas1, but at lower frequency. Human tumors of the soft tissue are a very complex and heterogeneous group of neoplasias. Our novel findings in genetically altered mice suggest that activation of the HIF signaling pathway could be an important pathogenetic event in the development and progression of at least a subset of these tumors.
Published: 2015

20. Adhesion, proliferation and osteogenic differentiation of human MSCs cultured under perfusion with a marine oxygen carrier on an allogenic bone substitute

Author: Franck Zal, Valérie Polard, Gaëlle Richard, Fiona Le Pape, Pascal Delépine, Emmanuelle Porchet, Claude Férec, Richard Pace, Pierre Weiss, Elisabeth Leize, Frédéric Dubrana, Sophie Sourice, Génétique, génomique fonctionnelle et biotechnologies (UMR 1078) (GGB), EFS-Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), HEMARINA SA, Etablissement Français du sang Bretagne (EFS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre hospitalier universitaire de Nantes (CHU Nantes), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), and Jehan, Frederic
Subjects: 0301 basic medicine, Scaffold, Materials science, Cell, Biomedical Engineering, Pharmaceutical Science, Medicine (miscellaneous), Biocompatible Materials, Osseointegration, Extracellular matrix, 03 medical and health sciences, Hemoglobins, 0302 clinical medicine, Perfusion Culture, Tissue engineering, Osteogenesis, medicine, Cell Adhesion, Animals, Humans, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Mesenchymal stem cell, Cell Differentiation, Mesenchymal Stem Cells, General Medicine, Anatomy, Cell biology, Oxygen, Perfusion, 030104 developmental biology, medicine.anatomical_structure, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Cell culture, 030220 oncology & carcinogenesis, Bone Substitutes, Biotechnology
Abstract: International audience; Tissue engineering strategies have been developed to optimize osseointegration in dental implant surgery. One of the major problems is the non-homogeneous spatial cell distribution in the scaffold, as well as subsequent matrix production. Insufficient nutrient and oxygen supplies inside the scaffold are factors in this phenomenon. To mediate this gradient formation, we have implemented a perfusion culture method to seed human bone marrow mesenchymal stem cells (MSCs) into three-dimensional (3-D)-allogenic bone scaffolds in combination with a marine haemoglobin, HEMOXCell®, for oxygen delivery. Cell culture was performed under static and perfusion conditions, with standard and osteogenic media, with and without HEMOXCell®. The cell seeding efficiency, as well as MSC/scaffold cytocompatibly were assessed using viability and proliferation assays. Scaffolds' cellularization and extracellular matrix (ECM) formation were analyzed using scanning electron microscopy and histological staining. Cell differentiation was investigated with osteogenic biomarkers gene expression analysis. The perfusion culture was observed to significantly promote MSC proliferation and differentiation throughout the scaffolds, especially when using the induction medium w/HEMOXCell®. Our data suggest that perfusion culture of MSC into allogenic bone substitute with HEMOXCell® as a natural oxygen carrier is promising for tissue engineering applications to oxygenate hypoxic areas and to promote cellular proliferation.
Published: 2017

21. Calcium supplementation decreases BCP-induced inflammatory processes in blood cells through the NLRP3 inflammasome down-regulation

Author: LAGADEC, Patricia, Balaguer, Thierry, Boukhechba, Florian, Michel, Grégory, Bouvet-Gerbettaz, Sébastien, Bouler, Jean-Michel, Scimeca, Jean-Claude, Rochet, Nathalie, Michel, Jean-Michel, Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Génétique, physiopathologie et ingénierie du tissu osseux (GéPITOS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Graftys SARL, Laboratoire de psychologie:Santé et qualité de vie, Université Bordeaux Segalen - Bordeaux 2, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), SCIMECA, Jean-Claude, Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)
Subjects: 0301 basic medicine, Inflammasomes, MESH: Dietary Supplements, 02 engineering and technology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, MESH: Monocytes, Biochemistry, Monocytes, Biphasic calcium phosphate, MESH: Down-Regulation, Mice, MESH: Inflammasomes, Gene expression, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], MESH: Animals, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], MESH: Hydroxyapatites, Whole blood, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Inflammasome, General Medicine, Hemolysis, Cell biology, Blood, MESH: NLR Family, Pyrin Domain-Containing 3 Protein, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, MESH: Calcium, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Hydroxyapatites, medicine.symptom, Biotechnology, medicine.drug, Materials science, 0206 medical engineering, Biomedical Engineering, Down-Regulation, chemistry.chemical_element, Inflammation, [SDV.CAN]Life Sciences [q-bio]/Cancer, Calcium, Biomaterials, 03 medical and health sciences, Downregulation and upregulation, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], NLR Family, Pyrin Domain-Containing 3 Protein, medicine, Animals, Humans, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Molecular Biology, MESH: Mice, Coagulation, MESH: Humans, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Monocyte proliferation, medicine.disease, 020601 biomedical engineering, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, 030104 developmental biology, chemistry, Dietary Supplements
Abstract: Interaction of host blood with biomaterials is the first event occurring after implantation in a bone defect. This study aimed at investigating the cellular and molecular consequences arising at the interface between whole blood and biphasic calcium phosphate (BCP) particles. We observed that, due to calcium capture, BCP inhibited blood coagulation, and that this inhibition was reversed by calcium supplementation. Therefore, we studied the impact of calcium supplementation on BCP effects on blood cells. Comparative analysis of BCP and calcium supplemented-BCP (BCP/Ca) effects on blood cells showed that BCP as well as BCP/Ca induced monocyte proliferation, as well as a weak but significant hemolysis. Our data showed for the first time that calcium supplementation of BCP microparticles had anti-inflammatory properties compared to BCP alone that induced an inflammatory response in blood cells. Our results strongly suggest that the anti-inflammatory property of calcium supplemented-BCP results from its down-modulating effect on P2X7R gene expression and its capacity to inhibit ATP/P2X7R interactions, decreasing the NLRP3 inflammasome activation. Considering that monocytes have a vast regenerative potential, and since the excessive inflammation often observed after bone substitutes implantation limits their performance, our results might have great implications in terms of understanding the mechanisms leading to an efficient bone reconstruction. Statement of significance Although scaffolds and biomaterials unavoidably come into direct contact with blood during bone defect filling, whole blood-biomaterials interactions have been poorly explored. By studying in 3D the interactions between biphasic calcium phosphate (BCP) in microparticulate form and blood, we showed for the first time that calcium supplementation of BCP microparticles (BCP/Ca) has anti-inflammatory properties compared to BCP-induced inflammation in whole blood cells and provided information related to the molecular mechanisms involved. The present study also showed that BCP, as well as BCP/Ca particles stimulate monocyte proliferation. As monocytes represent a powerful target for regenerative therapies and as an excessive inflammation limits the performance of biomaterials in bone tissue engineering, our results might have great implications to improve bone reconstruction.
Published: 2017

22. Gallium enhances reconstructive properties of a calcium phosphate bone biomaterial

Author: Jean-Michel Bouler, Ivana Strazic Geljic, Pascal Janvier, Jean-Claude Scimeca, Nicolas Melis, Florian Boukhechba, Charlotte Mellier, Sébastien Schaub, Elise Verron, Jean-Pierre Laugier, Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), GRAFTYS SA [Aix en Provence, France], Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre Commun de Microscopie Appliquée [Nice] (CCMA), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Nice University, CNRS, Graftys SA (Aix‐en‐Provence, France) and INSERM supported this work.This work was sponsored by Graftys SA, Convention CIFRE N°2011/1356., Jehan, Frederic, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Graftys SARL, Institute of Developmental Biology and Cancer (IBDC), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Centre Commun de Microscopie Appliquée (CCMA), and COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)
Subjects: Calcium Phosphates, 0301 basic medicine, Medicine (miscellaneous), bone reconstruction, bone repair quantification, calcium phosphate cements, gallium, gallium bone delivery, osteoclast/osteoblast crosstalk, second harmonic generation quantitative analysis, Biocompatible Materials, Gallium, Bone tissue, Bone remodeling, 0302 clinical medicine, Apatites, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Bone cell, Femur, Cells, Cultured, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Chemistry, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Bone Cements, Biomaterial, Cell Differentiation, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], 3. Good health, medicine.anatomical_structure, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, 030220 oncology & carcinogenesis, Biomedical Engineering, [SDV.CAN]Life Sciences [q-bio]/Cancer, Bone healing, Bone resorption, Osseointegration, Biomaterials, 03 medical and health sciences, Osteoclast, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], medicine, Animals, Humans, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Cell Shape, Cell Proliferation, Osteoblasts, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Rats, 030104 developmental biology, Bone Substitutes, Biomedical engineering
Abstract: International audience; Calcium phosphate (CaP)-based biomaterials are commonly used in bone reconstructive surgery to replace the damaged tissue, and can also serve as vectors for local drug delivery. Due to its inhibitory action on osteoclasts, the semi-metallic element gallium (Ga) is used for the systemic treatment of disorders associated with accelerated bone resorption. As it was demonstrated that Ga could be incorporated in the structure of CaP biomaterials, we investigated the biological properties of Ga-loaded CaP biomaterials. Culturing bone cells on Ga-CaP, we observed a decrease in osteoclast number and a downregulation of late osteoclastic markers expression, while Ga-CaP upregulated the expression of osteoblastic marker genes involved in the maturation of bone matrix. We next investigated in vivo bone reconstructive properties of different Ga-loaded biomaterials using a murine bone defect healing model. All implanted biomaterials showed a good osseointegration into the surrounding host tissue, accompanied by a successful bone ingrowth and bone marrow reconstruction, as evidenced by histological analysis. Moreover, quantitative micro-computed tomography analysis of implants revealed that Ga enhanced total defect filling. Lastly, we took advantage for the first time of a particular mode of non-linear microscopy (second harmonic generation) to quantify in vivo bone tissue reconstruction within a CaP bone substitute. By doing so, we showed that Ga exerted a positive impact on mature organized collagen synthesis. As a whole, our data support the hypothesis that Ga represents an attractive additive to CaP biomaterials for bone reconstructive surgery. Copyright © 2017 John Wiley & Sons, Ltd.
Published: 2017

23. The predictive value of microbiological findings on teeth, internal and external implant portions in clinical decision making

Author: Mia Rakic, Boris Delibasic, David Peñarrocha, Sandro Radovanovic, Luigi Canullo, Juan Antonio Blaya, Private Practice [Rome, Italy], Centre for Business Decision-making [Belgrade, Serbia] (Faculty of Organizational Sciences), University of Belgrade [Belgrade], Department of Oral Surgery [Valencia, Spain], Universitat de València (UV), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute for Biological Research Sinisa Stankovic [Belgrade] (IBISS), This study was supported by the Ministry of Education and Science, Republic of Serbia (project references #41008 and #173056). The study was partially supported by SUNSTAR SPAIN (which provided to cover expenses for part of the PCR assays used in the study) and by Clinident Institute who provided the microbiological analysis., and Jehan, Frederic
Subjects: 0301 basic medicine, Male, Peri-implantitis, Gingival and periodontal pocket, Alveolar Bone Loss, Dentistry, 0302 clinical medicine, Radiography, Dental, Medicine, periodontitis, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Middle Aged, Predictive value, 3. Good health, Dental Implantation, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Female, Oral Surgery, Infection, peri-implantitis, Adult, Decision trees, Clinical Decision-Making, Dental Plaque, Dental plaque, Real-Time Polymerase Chain Reaction, 03 medical and health sciences, Humans, Periodontal Pocket, Periodontitis, Parvimonas micra, Data mining, Aged, Dental Implants, decision trees, business.industry, Case-control study, data mining, infection, 030206 dentistry, medicine.disease, 030104 developmental biology, Case-Control Studies, Implant, business, Tooth
Abstract: International audience; Aim: The primary aim of this study was to evaluate 23 pathogens associated with peri-implantitis at inner part of implant connections, in peri-implant and periodontal pockets between patients suffering peri-implantitis and participants with healthy peri-implant tissues; the secondary aim was to estimate the predictive value of microbiological profile in patients wearing dental implants using data mining methods.Material and Methods: Fifty participants included in the present case─control study were scheduled for collection of plaque samples from the peri-implant pockets, internal connection, and periodontal pocket. Real-time polymerase chain reaction was performed to quantify 23 pathogens. Three predictive models were developed using C4.5 decision trees to estimate the predictive value of microbiological profile between three experimental sites.Results: The final sample included 47 patients (22 healthy controls and 25 diseased cases), 90 implants (43 with healthy peri-implant tissues and 47 affected by peri-implantitis). Total and mean pathogen counts at inner portions of the implant connection, in peri-implant and periodontal pockets were generally increased in peri-implantitis patients when compared to healthy controls. The inner portion of the implant connection, the periodontal pocket and peri-implant pocket, respectively, presented a predictive value of microbiologic profile of 82.78%, 94.31%, and 97.5% of accuracy.Conclusion: This study showed that microbiological profile at all three experimental sites is differently characterized between patients suffering peri-implantitis and healthy controls. Data mining analysis identified Parvimonas micra as a highly accurate predictor of peri-implantitis when present in peri-implant pocket while this method generally seems to be promising for diagnosis of such complex infections.
Published: 2017

24. In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line

Author: Moreau , Emmanuel, Rondon , Aurélie, Ty , Nancy, Bequignat , Jean-Baptiste, Quintana , Mercédès, Briat , Arnaud, Witkowski , Tiffany, Bouchon , Bernadette, Boucheix , Claude, Miot-Noirault , Elisabeth, Pouget , Jean-Pierre, Chezal , Jean-Michel, Navarro-Teulon , Isabelle, Degoul , Françoise, Eljezi , Teuta, Pinta , Pierre, Richard , Damien, Pinguet , Jérémy, Chagnon , Marie-Christine, Sautou , Valérie, Grimandi , Gaël, Imagerie Moléculaire et Stratégies Théranostiques - Clermont Auvergne ( IMoST ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Clermont Auvergne ( UCA ), Imagerie Moléculaire et Thérapie Vectorisée ( IMTV ), ITMO ' Technologies pour la Santé '-Cancéropôle CLARA-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université d'Auvergne - Clermont-Ferrand I ( UdA ), Radiopharmaceutiques biocliniques, Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR130, Image Science for Interventional Techniques ( ISIT ), Université d'Auvergne - Clermont-Ferrand I ( UdA ) -Clermont Université-Centre National de la Recherche Scientifique ( CNRS ), Inserm UMR-S 1004, Université Paris-Sud - Paris 11 ( UP11 ), Centre Jean Perrin, CRLCC Jean Perrin, Institut de recherche en cancérologie de Montpellier ( IRCM ), Université Montpellier 1 ( UM1 ) -CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Montpellier ( UM ), UMR 990, Université d'Auvergne - Clermont-Ferrand I ( UdA ) -Institut National de la Santé et de la Recherche Médicale, Laboratoire d'ingénierie osteo-articulaire et dentaire ( LIOAD ), Université de Nantes ( UN ) -IFR26-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut de Recherche en Gestion et en Economie ( IREGE ), Université Savoie Mont Blanc ( USMB [Université de Savoie] [Université de Chambéry] ), Service de Pharmacologie Médicale [Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Service de Pharmacie Centre Hospitalier Universitaire, Matériaux d'intérêt biologique, and Université de Nantes ( UN ) -Institut National de la Santé et de la Recherche Médicale ( INSERM )
Subjects: [ CHIM.ORGA ] Chemical Sciences/Organic chemistry, [ CHIM.COOR ] Chemical Sciences/Coordination chemistry, [ CHIM.THER ] Chemical Sciences/Medicinal Chemistry, [ CHIM.RADIO ] Chemical Sciences/Radiochemistry, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

25. Enriching a cellulose hydrogel with a biologically active marine exopolysaccharide for cell-based cartilage engineering

Author: Rederstorff, E., Réthoré, Gildas, Weiss, Pierre, Sourice, Sophie, Beck-Cormier, Sarah, Mathieu, E., Maillasson, Mike, Jacques, Yannick, Colliec-Jouault, S., Fellah, B., Guicheux, Jérome, VINATIER, Claire, Bernardo, Elizabeth, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Biotechnologie et Molécules Marines (BMM), Biotechnologies et Ressources Marines (BRM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes] (Pôle hospitalo-universitaire PHU4 - OTONN), Centre hospitalier universitaire de Nantes (CHU Nantes), Unité de recherche de l'institut du thorax (ITX-lab), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Immunobiology of Human αβ and γδ T Cells and Immunotherapeutic Applications (CRCINA-ÉQUIPE 1), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), Plateforme IMPACT Nantes (CRCINA-IMPACT), centre de Recherche et d'Investigation Préclinique [Nantes] (CRIP), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), E.R. received a fellowship from the Region Pays de la Loire through a Bioregos grant. This study was supported by grants from the ANR TecSan project Chondrograft, the Art hritis Fondation Courtin and the Société Française de Rhumatologie., Laboratoire de biotechnologie et des molécules marines [IFREMER, Nantes], Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), and Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)
Subjects: body regions, [SDV.CAN] Life Sciences [q-bio]/Cancer, marine polysaccharides, tissue engineering, technology, industry, and agriculture, glycosaminoglycan, chondrocytes, [SDV.CAN]Life Sciences [q-bio]/Cancer, hydrogel, cartilage
Abstract: International audience; The development of biologically and mechanically competent hydrogels is a prerequisite in cartilage engineering. We recently demonstrated that a marine exopolysaccharide, GY785, stimulates the in vitro chondrogenesis of adipose stromal cells. In the present study, we thus hypothesized that enriching our silated hydroxypropyl methylcellulose hydrogel (Si-HPMC) with GY785 might offer new prospects in the development of scaffolds for cartilage regeneration. The interaction properties of GY785 with growth factors was tested by surface plasmon resonance (SPR). The biocompatibility of Si-HPMC/GY785 towards rabbit articular chondrocytes (RACs) and its ability to maintain and recover a chondrocytic phenotype were then evaluated in vitro by MTS assay, cell counting and qRT-PCR. Finally, we evaluated the potential of Si-HPMC/GY785 associated with RACs to form cartilaginous tissue in vivo by transplantation into the subcutis of nude mice for 3 weeks. Our SPR data indicated that GY785 was able to physically interact with BMP-2 and TGFβ. Our analyses also showed that three-dimensionally (3D)-cultured RACs into Si-HPMC/GY785 strongly expressed type II collagen (COL2) and aggrecan transcripts when compared to Si-HPMC alone. In addition, RACs also produced large amounts of extracellular matrix (ECM) containing glycosaminoglycans (GAG) and COL2. When dedifferentiated RACs were replaced in 3D in Si-HPMC/GY785, the expressions of COL2 and aggrecan transcripts were recovered and that of type I collagen decreased. Immunohistological analyses of Si-HPMC/GY785 constructs transplanted into nude mice revealed the production of a cartilage-like extracellular matrix (ECM) containing high amounts of GAG and COL2. These results indicate that GY785-enriched Si-HPMC appears to be a promising hydrogel for cartilage tissue engineering.
Published: 2017

26. Neu5Gc and α1-3 GAL Xenoantigen Knockout Does Not Affect Glycemia Homeostasis and Insulin Secretion in Pigs

Author: Andrea Perota, Pierre Weiss, Nicolas Gaide, Steffi Bosch, Anne Moure, Jean-Paul Concordet, Emanuele Cozzi, Mathilde Mosser, Cesare Galli, Jérôme Guicheux, Jeremy Hervouet, David Minault, Jean-Marie Bach, Jean-Paul Soulillou, Jérôme Abadie, Sylvie Pogu, Apolline Salama, Xavier Lévêque, Olivier Gauthier, Dominique Jégou, Roberto Duchi, Gilles Blancho, Jean-Paul Judor, Sophie Brouard, Stéphanie Le Bas-Bernardet, Giovanna Lazzari, Ludmilla Le Berre, Sophie Conchon, David Riochet, Corentin Danna, Irina Lagutina, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Immuno-Endocrinologie Cellulaire et Moléculaire [Nantes] (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Société d'Accélération du Transfert de Technologies (SATT OUEST VALORISATION), Avantea Laboratory of Reproductive Technologies, Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Animaux Modèles pour la Recherche en Oncologie [Nantes] (AMaROC), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Muséum national d'Histoire naturelle (MNHN), Structure et Instabilité des Génomes (STRING), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), PHU 4 OTONN, Centre hospitalier universitaire de Nantes (CHU Nantes), CIC - Nantes, Avantea Foundation, Transplantation Immunology Unit, Universita degli Studi di Padova, Consortium for Research in Organ Transplantation (CORIT), University of Bologna, Université de Nantes (UN), Avantea Laboratory of Reproductive Technologies [Cremona, Italy], Département de Chirurgie Expérimentale [Nantes] (Oniris - Centre de recherche et d'investigation préclinique), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre de recherche et d'investigation préclinique [Nantes], Museum National d'Histoire Naturelle de Paris, Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Département de pédiatrie [CHU Nantes], Université de Nantes - UFR Odontologie, Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes] (Pôle hospitalo-universitaire PHU4 - OTONN), CIC biothérapies CBT 0503 [Nantes], Hôtel-Dieu de Nantes-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes), Avantea Foundation [Cremona, Italy], Transplantation Immunology Unit [Padua, Italy] (Department of Transfusion Medicine), University of Padua–Ospedale Giustinianeo [Padua, Italy], Consortium for Research in Organ Transplantation [Padua, Italy] (CORIT), Department of Veterinary Medical Sciences [Ozzano dell'Emilia, Italy], Pays de la Loire Region (France) (Xenothera academic program and to A.S.), the Société d’Accélération du Transfert de Technologies Ouest Valorisation (to A.S.)., ANR: ANR-10IBHU-005, ANR: ANR-II-INSB-0014, European Project: LSHB-CT-2006-037377, European Project: 603049, European Project: ECTIS, Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Recherche Agronomique (INRA), Animaux modèles pour la recherche en oncologie comparée (AMaROC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN), Institut de Transplantation Urologie Néphrologie, AMaROC - Oniris Nantes, Centre d'Investigation Clinique (CIC) Biotherapy, This work was supported by Pays de la Loire Region (France) (Xenothera academic program and to A.S.), the Société d’Accélération du Transfert de Technologies Ouest Valorisation (to A.S.), the European Center for Transplantation and Immunotherapy Sciences (ECTIS IHU, Nantes, France), the National Research Agency 'Investment Into The Future' programs (ANR-10-IBHU-005, to X.L., and ANR-II-INSB-0014), the European Commission’s Xenome Sixth Framework Programme (LSHB-CT-2006-037377), and the European Seventh Framework Programme 'Translink' research program (grant agreement 603049 and to L.L.B.)., ANR-10-IBHU-0005,CESTI (TSI-IHU),Centre Européen des Sciences de la Transplantation et de l'Immunothérapie (TSI-IHU)(2010), ANR-11-INBS-0014,TEFOR,Transgenèse pour les Etudes Fonctionnelles sur les Organismes modèles(2011), Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Padova = University of Padua (Unipd), Consorzio per la Ricerca sul Trapianto d'Organ = Consortium for Research in Organ Transplantation (CORIT), University of Bologna/Università di Bologna, European Project: 603049,EC:FP7:HEALTH,FP7-HEALTH-2013-INNOVATION-1,TRANSLINK(2013), Mosser, Mathilde, Lévêque, Xavier, Perota, Andrea, Galli, Cesare, Soulillou, Jean-Paul, Bach, Jean-Marie, Jehan, Frederic, Instituts Hospitalo-Universitaires B - Centre Européen des Sciences de la Transplantation et de l'Immunothérapie (TSI-IHU) - - CESTI (TSI-IHU)2010 - ANR-10-IBHU-0005 - IBHU - VALID, Infrastructures - Transgenèse pour les Etudes Fonctionnelles sur les Organismes modèles - - TEFOR2011 - ANR-11-INBS-0014 - INBS - VALID, and Defining the role of xeno-directed and autoimmune events in patients receiving animal-derived bioprosthetic heart valves - TRANSLINK - - EC:FP7:HEALTH2013-09-01 - 2017-08-31 - 603049 - VALID
Subjects: Blood Glucose, Male, 0301 basic medicine, Swine, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Islets of Langerhans Transplantation, 030230 surgery, Gene Knockout Techniques, 0302 clinical medicine, Insulin Secretion, Homeostasis, Insulin, Glucose homeostasis, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Heterologous, geography.geographical_feature_category, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, C-Peptide, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Islet, 3. Good health, Heterophile, medicine.anatomical_structure, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Type 1, medicine.medical_specialty, endocrine system, Transplantation, Heterologous, Animals, Antigens, Heterophile, Diabetes Mellitus, Type 1, Galactose, Glucagon, Glucose, Islets of Langerhans, Neuraminic Acids, Pancreas, Purinergic P1 Receptor Antagonists, Theophylline, Carbohydrate metabolism, Biology, 03 medical and health sciences, Internal medicine, Diabetes mellitus, Diabetes Mellitus, Internal Medicine, medicine, Antigens, Transplantation, Type 1 diabetes, geography, Pancreatic islets, medicine.disease, 030104 developmental biology, Endocrinology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Xenocell therapy from neonate or adult pig pancreatic islets is one of the most promising alternatives to allograft in type 1 diabetes for addressing organ shortage. In humans, however, natural and elicited anti-bodies specific for pig xenoantigens, α-(1,3)-galactose (GAL) and N-glycolylneuraminic acid (Neu5Gc), are likely to significantly contribute to xenoislet rejection. We obtained double-knockout (DKO) pigs lacking GAL and Neu5Gc. Because Neu5Gc 2/2 mice exhibit glycemic dys-regulations and pancreatic b-cell dysfunctions, we evaluated islet function and glucose metabolism regulation in DKO pigs. Isolation of islets from neonate piglets yielded identical islet equivalent quantities to quantities obtained from control wild-type pigs. In contrast to wild-type islets, DKO islets did not induce anti-Neu5Gc antibody when grafted in cytidine monophosphate-N-acetylneuraminic acid hydroxylase KO mice and exhibited in vitro normal insulin secretion stimulated by glucose and theophylline. Adult DKO pancreata showed no histological abnormalities , and immunostaining of insulin and glucagon was similar to that from wild-type pancreata. Blood glucose, insulin, C-peptide, the insulin-to-glucagon ratio, and HOMA-insulin resistance in fasted adult DKO pigs and blood glucose and C-peptide changes after intravenous glucose or insulin administration were similar to wild-type pigs. This first evaluation of glucose homeostasis in DKO pigs for two major xenoantigens paves the way to their use in (pre)clinical studies.
Published: 2017

27. Bone marrow cell extract promotes the regeneration of irradiated bone

Author: Michel, Guillaume, Bléry, Pauline, Henoux, Michaël, Guicheux, Jérôme, Weiss, Pierre, Brouard, Sophie, Malard, Olivier, Espitalier, Florent, Jehan, Frederic, Service d’ORL et de chirurgie cervico-faciale [CHU Hôtel Dieu, Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), and The authors received grants from 'Ligue contre le cancer' foundation. For the whole project, we received 20 000 euros (https://www.ligue-cancer.net/).
Subjects: [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology
Abstract: International audience; Mandibular osteoradionecrosis is a severe side effect of radiotherapy after the treatment of squamous cell carcinomas of the upper aerodigestive tract. As an alternative to its treatment by micro-anastomosed free-flaps, preclinical tissular engineering studies have been developed. Total bone marrow (TBM) associated with biphasic calcium phosphate (BCP) significantly enhanced bone formation in irradiated bone. One mechanism, explaining how bone marrow cells can help regenerate tissues like this, is the paracrine effect. The bone marrow cell extract (BMCE) makes use of this paracrine mechanism by keeping only the soluble factors such as growth factors and cytokines. It has provided significant results in repairing various tissues, but has not yet been studied in irradiated bone reconstruction. The purpose of this study was to evaluate the effect of BMCE via an intraosseous or intravenous delivery, with a calcium phosphate scaffold, in irradiated bone reconstruction. Twenty rats were irradiated on their hind limbs with a single 80-Gy dose. Three weeks later, surgery was performed to create osseous defects. The intraosseous group (n = 12) studied the effect of BMCE in situ, with six combinations (empty defect, BCP, TBM, BCP-TBM, lysate only, BCP-lysate). After four different combinations of implantation (empty defect, BCP, TBM, BCP-TBM), the intravenous group (n = 8) received four intravenous injections of BMCE for 2 weeks. Five weeks after implantation, samples were explanted for histological and scanning electron microscopy analysis. Lysate immunogenicity was studied with various mixed lymphocyte reactions. Intravenous injections of BMCE led to a significant new bone formation compared to the intraosseous group. The BCP-TBM mixture remained the most effective in the intraosseous group. However, intravenous injections were more effective, with TBM placed in the defect, with or without biomaterials. Histologically, highly cellularized bone marrow was observed in the defects after intravenous injections, and not after an in situ use of the lysate. The mixed lymphocyte reactions did not show any proliferation after 3, 5, or 7 days of lysate incubation with lymphocytes from another species.This study evaluated the role of BMCE in irradiated bone reconstruction. There were significant results arguing in favor of BMCE intravenous injections. This could open new perspectives to irradiated bone reconstruction.
Published: 2017

28. Pharmacologically active microcarriers delivering BDNF within a hydrogel: Novel strategy for human bone marrow-derived stem cells neural/neuronal differentiation guidance and therapeutic secretome enhancement

Author: Paul C. Schiller, Marie Claire Venier-Julienne, Jérôme Guicheux, Xavier Garric, Claudia N. Montero-Menei, Anne des Rieux, Saikrishna Kandalam, Fabien Violet, Gaëtan J.-R. Delcroix, Emilie M. André, Laurence Sindji, Micro et Nanomédecines Translationnelles (MINT), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), GRECC and Research Service [Miami, FL, USA], Veterans Affairs Medical Center [Miami], Department of Orthopaedics, Tissue Bank, Geriatrics and Interdisciplinary Stem Cell Institutes [Miami, FL, USA], University of Miami Leonard M. Miller School of Medicine (UMMSM), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Department of Biochemistry & Molecular Biology [Miami, FL, USA], Geriatrics Institute [Miami, FL, USA], Advanced Drug Delivery and Biomaterials [Brussels, Belgium], Université Catholique de Louvain = Catholic University of Louvain (UCL)-Louvain Drug Research Institute (LDRI), Institut de la matière condensée et des nanosciences / Institute of Condensed Matter and Nanosciences (IMCN), Université Catholique de Louvain = Catholic University of Louvain (UCL), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes] (Pôle hospitalo-universitaire PHU4 - OTONN), Centre hospitalier universitaire de Nantes (CHU Nantes), Groupe STEP (Inserm U791 - LIOAD), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Project funded by the European Commission, Education, Audiovisual and Culture Executive Agency (EACEA), Erasmus Mundus programme, NanoFar doctorate and by ‘‘Région Pays de La Loire'., and Jehan, Frederic
Subjects: Male, 0301 basic medicine, Chemokine, Proteome, medicine.medical_treatment, Biocompatible Materials, Biochemistry, Regenerative medicine, Hydrogel, Polyethylene Glycol Dimethacrylate, Hypromellose Derivatives, 0302 clinical medicine, Neurotrophic factors, Chemical Precipitation, Neurons, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, biology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Si-HPMC hydrogel, Cell Differentiation, General Medicine, Silanes, Microspheres, 3. Good health, Cell biology, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Stem cell, Rheology, Neural repair, Biotechnology, Biomedical Engineering, BDNF drug delivery, Context (language use), Neuroprotection, Biomaterials, 03 medical and health sciences, medicine, Humans, RNA, Messenger, Cell Shape, Molecular Biology, Aged, business.industry, Brain-Derived Neurotrophic Factor, Growth factor, Mesenchymal stem cell, Drug Liberation, 030104 developmental biology, Gene Expression Regulation, biology.protein, Nanoparticles, Mesenchymal stem cells, business, 030217 neurology & neurosurgery, Biomedical engineering
Abstract: Stem cells combined with biodegradable injectable scaffolds releasing growth factors hold great promises in regenerative medicine, particularly in the treatment of neurological disorders. We here integrated human marrow-isolated adult multilineage-inducible (MIAMI) stem cells and pharmacologically active microcarriers (PAMs) into an injectable non-toxic silanized-hydroxypropyl methylcellulose (Si-HPMC) hydrogel. The goal is to obtain an injectable non-toxic cell and growth factor delivery device. It should direct the survival and/or neuronal differentiation of the grafted cells, to safely transplant them in the central nervous system, and enhance their tissue repair properties. A model protein was used to optimize the nanoprecipitation conditions of the neuroprotective brain-derived neurotrophic factor (BDNF). BDNF nanoprecipitate was encapsulated in fibronectin-coated (FN) PAMs and the in vitro release profile evaluated. It showed a prolonged, bi-phasic, release of bioactive BDNF, without burst effect. We demonstrated that PAMs and the Si-HPMC hydrogel increased the expression of neural/neuronal differentiation markers of MIAMI cells after 1week. Moreover, the 3D environment (PAMs or hydrogel) increased MIAMI cells secretion of growth factors (b-NGF, SCF, HGF, LIF, PlGF-1, SDF-1α, VEGF-AD) and chemokines (MIP-1αβ, RANTES, IL-8). These results show that PAMs delivering BDNF combined with Si-HPMC hydrogel represent a useful novel local delivery tool in the context of neurological disorders. It not only provides neuroprotective BDNF but also bone marrow-derived stem cells that benefit from that environment by displaying neural commitment and an improved neuroprotective/reparative secretome. It provides preliminary evidence of a promising pro-angiogenic, neuroprotective and axonal growth-promoting device for the nervous system.Combinatorial tissue engineering strategies for the central nervous system are scarce. We developed and characterized a novel injectable non-toxic stem cell and protein delivery system providing regenerative cues for central nervous system disorders. BDNF, a neurotrophic factor with a wide-range effect, was nanoprecipitated to maintain its structure and released in a sustained manner from novel polymeric microcarriers. The combinatorial 3D support, provided by fibronectin-microcarriers and the hydrogel, to the mesenchymal stem cells guided the cells towards a neuronal differentiation and enhanced their tissue repair properties by promoting growth factors and cytokine secretion. The long-term release of physiological doses of bioactive BDNF, combined to the enhanced secretion of tissue repair factors from the stem cells, constitute a promising therapeutic approach.
Published: 2017

29. Substituts osseux

Author: Fabienne Jordana, Catherine Le Visage, Pierre Weiss, Hémostase, bio-ingénierie et remodelage cardiovasculaires (LBPC), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Galilée, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: 0301 basic medicine, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, 0206 medical engineering, 02 engineering and technology, General Medicine, 020601 biomedical engineering, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 030104 developmental biology, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

30. Position paper for the evaluation and management of oral status in patients with valvular disease: Groupe de Travail Valvulopathies de la Societe Francaise de Cardiologie, Societe Frangaise de Chinirgie Orale, Societe Frangaise de Parodontologie et d'Implantologie Orale, Societe Frangaise d'Endodontie et Societe de Pathologie Infectieuse de Langue Frangaise

Author: Philippe Lesclous, Sarah Millot, Marie-Laure Colombier, Philippe Tramba, Bernard Iung, Emmanuel Lansac, Clement Messeca, Mathieu Ballanger, Jean Luc Charrier, Christophe Tribouilloy, Xavier Duval, Fabien Doguet, Loredana Radoï, Stéphane Simon, Alain Berrebi, Gilbert Habib, Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Université Paris Descartes - Faculté de Chirurgie Dentaire (UPD5 Odontologie), Université Paris Descartes - Paris 5 (UPD5), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), Oral medicine and oral surgery department, Centre d'Investigation Clinique Antilles-Guyane (CIC - Antilles Guyane), Université des Antilles et de la Guyane (UAG)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de Fort de France-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Laboratoire Hospitalier et Universitaire Parasitologie Mycologie, Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Gynécologie‑Obstétrique, Hôpital Paule de Viguier, Service de chirurgie cadiovasculaire et thoracique [Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service de Cardiologie [Amiens], CHU Amiens-Picardie, Mécanismes physiologiques et conséquences des calcifications cardiovasculaires: rôle des remodelages cardiovasculaires et osseux, Université de Picardie Jules Verne (UPJV)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), CIC Hôpital Bichat, AP-HP - Hôpital Bichat - Claude Bernard [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-UFR de Médecine, DHU FIRE Centre de compétence des maladies pulmonaires rares, Service de cardiologie, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Sud - Paris 11 (UP11)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse], Institut National de la Santé et de la Recherche Médicale (INSERM)-UFR de Médecine-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université de Nantes - UFR Odontologie (UFR Odonto), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), Service Gynécologie [CHU Toulouse], Pôle Femme-Mère-Couple [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Hôpital Charles Nicolle [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), AP-HP - Hôpital Bichat - Claude Bernard [Paris], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-UFR de Médecine
Subjects: medicine.medical_specialty, medicine.diagnostic_test, business.industry, General surgery, valvular heart disease, Implant failure, Physical examination, 030206 dentistry, General Medicine, Disease, 030204 cardiovascular system & hematology, medicine.disease, 3. Good health, Surgery, 03 medical and health sciences, 0302 clinical medicine, Acute Endocarditis, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Infective endocarditis, medicine, Endocarditis, Antibiotic prophylaxis, Cardiology and Cardiovascular Medicine, business
Abstract: International audience; Oral health is of particular importance in patients with heart valve diseases because of the risk of infective endocarditis. Recommendations for antibiotic prophylaxis before dental procedures have been restricted, but the modalities of oral evaluation and dental care are not detailed in guidelines. Therefore, a multidisciplinary working group reviewed the literature to propose detailed approaches for the evaluation and management of buccodental status in patients with valvular disease. Simple questions asked by a non-dental specialist may draw attention to buccodental diseases. Besides clinical examination, recent imaging techniques are highly sensitive for the detection of inflammatory bone destruction directly related to oral or dental infection foci. The management of buccodental disease before cardiac valvular surgery should be adapted to the timing of the intervention. Simple therapeutic principles can be applied even before urgent intervention. Restorative dentistry and endodontic and periodontal therapy can be performed before elective valvular intervention and during the follow-up of patients at high risk of endocarditis. The detection and treatment of buccodental foci of infection should follow specific rules in patients who present with acute endocarditis. Implant placement is no longer contraindicated in patients at intermediate risk of endocarditis, and can also be performed in selected high-risk patients. The decision for implant placement should follow an analysis of general and local factors increasing the risk of implant failure. The surgical and prosthetic procedures should be performed in optimal safety conditions. It is therefore now possible to safely decrease the number of contraindicated dental procedures in patients at risk of endocarditis. (C) 2017 Elsevier Masson SAS. All rights reserved.
Published: 2017

31. Local control of the host immune response performed with mesenchymal stem cells: perspectives for functional intracerebral xenotransplantation

Author: Philippe Naveilhan, Tony Durand, Véronique Nerrière-Daguin, Reynald Thinard, Laetitia Kermarrec, Isabelle Neveu, Elodie Mathieux, Bernard Vanhove, Laurent Lescaudron, Xavier Lévêque, Thomas Haudebourg, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Faculté de Médecine - Université de Nantes, PRES Université Nantes Angers Le Mans (UNAM), Neuropathies du système nerveux entérique et pathologies digestives, implication des cellules gliales entériques, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), and Jehan, Frederic
Subjects: Male, Pathology, medicine.medical_treatment, Sus scrofa, Mesencephalon, Parkinson, Neurons, Immunity, Cellular, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, CD11b Antigen, immunosuppression, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Graft Survival, Brain, Immunosuppression, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, restorative strategies, Systemic administration, Molecular Medicine, Syngenic, Chemokines, rejection, Immunocompetence, medicine.medical_specialty, Cell Survival, Xenotransplantation, Molecular Sequence Data, Transplantation, Heterologous, T cells, Motor Activity, Biology, Mesenchymal Stem Cell Transplantation, survival, Immune system, Neuroblast, Glial Fibrillary Acidic Protein, medicine, Animals, RNA, Messenger, Oxidopamine, mesenchymal stem cells, Mesenchymal stem cell, Immunity, Recovery of Function, Original Articles, Cell Biology, Transplantation, Rats, Inbred Lew, brain transplantation
Abstract: International audience; Foetal pig neuroblasts are interesting candidates as a cell source for transplantation, but xenotransplantation in the brain requires the development of adapted immunosuppressive treatments. As systemic administration of high doses of cyclosporine A has side effects and does not protect xenotransplants forever, we focused our work on local control of the host immune responses. We studied the advantage of cotransplanting syngenic mesenchymal stem cells (MSC) with porcine neuroblasts (pNb) in immunocompetent rat striata. Two groups of animals were transplanted , either with pNb alone or with both MSC and pNb. At day 63, no porcine neurons were detected in the striata that received only pNb, while four of six rats transplanted with both pNb and MSC exhibited healthy porcine neurons. Interestingly, 50% of the cotransplanted rats displayed healthy grafts with pNF70+ and TH+ neurons at 120 days post-transplantation. qPCR analyses revealed a general dwindling of pro-and anti-inflammatory cytokines in the striata that received the cotransplants. Motor recovery was also observed following the transplantation of pNb and MSC in a rat model of Parkinson's disease. Taken together, the present data indicate that the immunosuppressive properties of MSC are of great interest for the long-term survival of xenogeneic neurons in the brain.
Published: 2014

32. Radiation endurance in Al2O3 nanoceramics

Author: P. Trocellier, C. Baumier, Rosaria Brescia, Lucile Beck, Pasquale Vena, Luca Ceseracciu, F. García Ferré, A. Mairov, Dario Gastaldi, Marco Beghi, Kumar Sridharan, Yves Serruys, F. Di Fonzo, O. Kaïtasov, Service de recherches de métallurgie physique (SRMP), Département des Matériaux pour le Nucléaire (DMN), CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) (CEA-DES (ex-DEN)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) (CEA-DES (ex-DEN)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Physique Nucléaire d'Orsay (IPNO), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Centre de Spectrométrie Nucléaire et de Spectrométrie de Masse (CSNSM), Centre d'Energétique et de Thermique de Lyon (CETHIL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), and Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, [PHYS.NUCL]Physics [physics]/Nuclear Theory [nucl-th], Oxide, 02 engineering and technology, [PHYS.NEXP]Physics [physics]/Nuclear Experiment [nucl-ex], 01 natural sciences, Article, law.invention, chemistry.chemical_compound, law, 0103 physical sciences, Radiation damage, Irradiation, Ceramic, Crystallization, Composite material, Thin film, nanoceramics, 010302 applied physics, Multidisciplinary, Radiation, 021001 nanoscience & nanotechnology, Grain growth, chemistry, visual_art, visual_art.visual_art_medium, 0210 nano-technology, Crystal twinning
Abstract: The lack of suitable materials solutions stands as a major challenge for the development of advanced nuclear systems. Most issues are related to the simultaneous action of high temperatures, corrosive environments and radiation damage. Oxide nanoceramics are a promising class of materials which may benefit from the radiation tolerance of nanomaterials and the chemical compatibility of ceramics with many highly corrosive environments. Here, using thin films as a model system, we provide new insights into the radiation tolerance of oxide nanoceramics exposed to increasing damage levels at 600 °C –namely 20, 40 and 150 displacements per atom. Specifically, we investigate the evolution of the structural features, the mechanical properties, and the response to impact loading of Al2O3 thin films. Initially, the thin films contain a homogeneous dispersion of nanocrystals in an amorphous matrix. Irradiation induces crystallization of the amorphous phase, followed by grain growth. Crystallization brings along an enhancement of hardness, while grain growth induces softening according to the Hall-Petch effect. During grain growth, the excess mechanical energy is dissipated by twinning. The main energy dissipation mechanisms available upon impact loading are lattice plasticity and localized amorphization. These mechanisms are available in the irradiated material, but not in the as-deposited films.
Published: 2016

33. Nanocomposite hydrogels for cartilage tissue engineering: mesoporous silica nanofibers interlinked with siloxane derived polysaccharide

Author: Frédéric Rambaud, Olivier Chauvet, Nela Buchtová, Jean Le Bideau, Pierre Weiss, Clément Sanchez, Philippe Belleville, Jérôme Guicheux, Karine Valle, Gildas Rethore, Cécile Boyer, Institut des Matériaux Jean Rouxel (IMN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), CEA Le Ripault (CEA Le Ripault), Direction des Applications Militaires (DAM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Chaire Chimie des matériaux hybrides, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), This work was funded by the Re´gion Pays de la Loire within the BIOREGOS 2 project., Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN), Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Collège de France (CdF (institution))-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Collège de France (CdF (institution))-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Jehan, Frederic
Subjects: Scaffold, Materials science, Siloxanes, Cell Survival, Nanofibers, Biomedical Engineering, Biophysics, Bioengineering, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Nanocomposites, Biomaterials, chemistry.chemical_compound, Polysaccharides, Materials Testing, Humans, Composite material, Porosity, Cells, Cultured, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Nanocomposite, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Tissue Engineering, Tissue Scaffolds, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, technology, industry, and agriculture, Hydrogels, Mesoporous silica, Silicon Dioxide, 021001 nanoscience & nanotechnology, [PHYS.COND.CM-MS] Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], 0104 chemical sciences, Cartilage, Cross-Linking Reagents, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Chemical engineering, chemistry, Siloxane, Nanofiber, Self-healing hydrogels, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], 0210 nano-technology, Hybrid material
Abstract: International audience; Injectable materials for mini-invasive surgery of cartilage are synthesized and thoroughly studied. The concept of these hybrid materials is based on providing high enough mechanical performances along with a good medium for chondrocytes proliferation. The unusual nanocomposite hydrogels presented herein are based on siloxane derived hydroxypropylmethylcellulose (Si-HPMC) interlinked with mesoporous silica nanofibers. The mandatory homogeneity of the nanocomposites is checked by fluorescent methods, which show that the silica nanofibres dispersion is realized down to nanometric scale, suggesting an efficient immobi-lization of the silica nanofibres onto the Si-HPMC scaffold. Such dispersion and immobilization are reached thanks to the chemical affinity between the hydrophilic silica nanofibers and the pendant silanolate groups of the Si-HPMC chains. Tuning the amount of nanocharges allows tuning the resulting mechanical features of these injectable biocom-patible hybrid hydrogels. hASC stem cells and SW1353 chondrocytic cells viability is checked within the nanocom-posite hydrogels up to 3 wt% of silica nanofibers.
Published: 2013

34. Expression of phosphate transporters in optimized cell culture models for dental cells biomineralization

Author: Merametdjian, Laure, David, Amandine, Bon, Nina, Couasnay, Greig, Jerome Guicheux, Gaucher, Celine, Beck-Cormier, Sarah, Beck, Laurent, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), PHU 4 OTONN, Centre hospitalier universitaire de Nantes (CHU Nantes), Pathologies, Imagerie et Biothérapies oro-faciales (EA 2496), Université Paris Descartes - Paris 5 (UPD5), Groupe hospitalier Albert Chenevier – Henri Mondor, This study was supported by an IFRO (InstitutFrançais de la Recherche Odontologique)grant., IFRO (Institut Français de Recherche en Odontologie), and Jehan, Frederic
Subjects: [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, stomatognathic diseases, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, stomatognathic system, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, phosphate, mineralization, ALC, M2H4, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, mineralization, ALC, M2H4, phosphate
Abstract: International audience; Phosphate is a key component of dental mineral composition. The physiological role of membrane proteins of dental cells is suspected to be crucial for mineralization mechanisms. Contrary to published data related to calcium, data on regulation of phosphate flux through membrane of mineralizing cells are scarce. To address this lack of data, we studied the expression of six membranous phosphate transporters in two dental cell lines: a rat odontoblastic cell line (M2H4) and a mouse ameloblastic cell line (ALC) for which we optimized the mineralizing culture conditions. La place essentielle du phosphate dans la composante minérale de la dent laisse sup-poser un rôle physiologique déterminant pour les protéines membranaires permettant son entrée dans les cellules dentaires. Contraire-ment à celles disponibles pour le calcium, les données sur les molécules permettant la production et la régulation du flux de phosphate aux sites de formation du minéral par les ce-llules minéralisantes de la dent sont peu con-nues. Nous avons analysé dans cette étude six transporteurs de phosphate membranai-res dans deux lignées cellulaires dentaires: une lignée d'odontoblastes de rat (M2H4) et une lignée améloblastique murine, les ALC, pour laquelle nous avons optimisé les conditions de culture minéralisantes.
Published: 2016

35. Skeletal Mineralization Deficits and Impaired Biogenesis and Function of Chondrocyte-Derived Matrix Vesicles in Phospho1(-/-) and Phospho1/Pi t1 Double-Knockout Mice

Author: Yadav, Manisha, Bottini, Massimo, Cory, Esther, Bhattacharya, Kunal, Kuss, Pia, Narisawa, Sonoko, Sah, Robert, Beck, Laurent, Fadeel, Bengt, Farquharson, Colin, Millán, José Luis, Beck, Laurent, Sanford Burnham Prebys Medical Discovery Institute, Università degli Studi di Roma Tor Vergata [Roma], University of California [San Diego] (UC San Diego), University of California (UC), Karolinska Institutet [Stockholm], Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Edinburgh, and University of California
Subjects: [SDV]Life Sciences [q-bio], Knockout, GENETIC ANIMAL MODELS, MOLECULAR PATHWAYS, Medical and Health Sciences, Article, Calcification, Type III, Mice, DEVELOPMENT, Calcification, Physiologic, Chondrocytes, Rare Diseases, Engineering, Bone Density, Animals, Settore BIO/10, Dental/Oral and Craniofacial Disease, Physiologic, Mice, Knockout, Pediatric, Sodium-Phosphate Cotransporter Proteins, Type III, Sodium-Phosphate Cotransporter Proteins, GROWTH PLATE, Biological Sciences, Anatomy & Morphology, Phosphoric Monoester Hydrolases, [SDV] Life Sciences [q-bio], Musculoskeletal, Congenital Structural Anomalies
Abstract: International audience; We have previously shown that ablation of either the Phospho1 or Alpl gene, encoding PHOSPHO1 and tissue-nonspecific alkaline phosphatase (TNAP) respectively, lead to hyperosteoidosis, but that their chondrocyte-derived and osteoblast-derived matrix vesicles (MVs) are able to initiate mineralization. In contrast, the double ablation of Phospho1 and Alpl completely abolish initiation and progression of skeletal mineralization. We argued that MVs initiate mineralization by a dual mechanism: PHOSPHO1-mediated intravesicular generation of inorganic phosphate (Pi ) and phosphate transporter-mediated influx of Pi . To test this hypothesis, we generated mice with col2a1-driven Cre-mediated ablation of Slc20a1, hereafter referred to as Pi t1, alone or in combination with a Phospho1 gene deletion. Pi t1(col2/col2) mice did not show any major phenotypic abnormalities, whereas severe skeletal deformities were observed in the [Phospho1(-/-) ; Pi t1(col2/col2) ] double knockout mice that were more pronounced than those observed in the Phospho1(-/-) mice. Histological analysis of [Phospho1(-/-) ; Pi t1(col2/col2) ] bones showed growth plate abnormalities with a shorter hypertrophic chondrocyte zone and extensive hyperosteoidosis. The [Phospho1(-/-) ; Pi t1(col2/col2) ] skeleton displayed significant decreases in BV/TV%, trabecular number, and bone mineral density, as well as decreased stiffness, decreased strength, and increased postyield deflection compared to Phospho1(-/-) mice. Using atomic force microscopy we found that ∼80% of [Phospho1(-/-) ; Pi t1(col2/col2) ] MVs were devoid of mineral in comparison to ∼50% for the Phospho1(-/-) MVs and ∼25% for the WT and Pi t1(col2/col2) MVs. We also found a significant decrease in the number of MVs produced by both Phospho1(-/-) and [Phospho1(-/-) ; Pi t1(col2/col2) ] chondrocytes. These data support the involvement of phosphate transporter 1, hereafter referred to as Pi T-1, in the initiation of skeletal mineralization and provide compelling evidence that PHOSPHO1 function is involved in MV biogenesis. © 2016 American Society for Bone and Mineral Research.
Published: 2016

36. Periostin-deficient mice, a relevant animal model to investigate periodontitis or not?

Author: Philippe Lesclous, Olivier Lapérine, Jérôme Guicheux, Jehan, Frederic, Université de Nantes - UFR Odontologie, Université de Nantes (UN), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes] (Pôle hospitalo-universitaire PHU4 - OTONN), and Centre hospitalier universitaire de Nantes (CHU Nantes)
Subjects: 0301 basic medicine, Periodontitis, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, business.industry, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, 030206 dentistry, Periostin, medicine.disease, Article, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Animal model, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Immunology, Deficient mouse, General Earth and Planetary Sciences, Medicine, business, ComputingMilieux_MISCELLANEOUS, General Environmental Science
Abstract: International audience
Published: 2016

37. An Analysis of Block Sampling Strategies in Compressed Sensing

Author: Claire Boyer, Pierre Weiss, Jérémie Bigot, Institut de Mathématiques de Toulouse UMR5219 (IMT), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse 1 Capitole (UT1)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), SAD Paysage (SAD Paysage), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Mathématiques, Informatique, Automatique (DMIA), Institut Supérieur de l'Aéronautique et de l'Espace (ISAE-SUPAERO), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut des Technologies Avancées en sciences du Vivant (ITAV), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut de Mathématiques de Bordeaux (IMB), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1-Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux (Bordeaux INP)-Centre National de la Recherche Scientifique (CNRS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: FOS: Computer and information sciences, Gaussian, Computer Science - Information Theory, Mathematics - Statistics Theory, 02 engineering and technology, Iterative reconstruction, Statistics Theory (math.ST), Library and Information Sciences, Signal, symbols.namesake, Compressed Sensing, [MATH.MATH-ST]Mathematics [math]/Statistics [math.ST], 0202 electrical engineering, electronic engineering, information engineering, FOS: Mathematics, Coherence (signal processing), Computer vision, ComputingMilieux_MISCELLANEOUS, Mathematics, Block (data storage), blocks of measurements, Signal reconstruction, business.industry, Information Theory (cs.IT), Sampling (statistics), [MATH.MATH-IT]Mathematics [math]/Information Theory [math.IT], 020206 networking & telecommunications, [STAT.TH]Statistics [stat]/Statistics Theory [stat.TH], exact recovery, Computer Science Applications, Compressed sensing, [INFO.INFO-IT]Computer Science [cs]/Information Theory [cs.IT], symbols, 020201 artificial intelligence & image processing, sampling continuous trajectories, Artificial intelligence, $\ell^1$ minimization, business, Algorithm, Information Systems
Abstract: Compressed sensing is a theory which guarantees the exact recovery of sparse signals from a small number of linear projections. The sampling schemes suggested by current compressed sensing theories are often of little practical relevance, since they cannot be implemented on real acquisition systems. In this paper, we study a new random sampling approach that consists of projecting the signal over blocks of sensing vectors. A typical example is the case of blocks made of horizontal lines in the 2-D Fourier plane. We provide the theoretical results on the number of blocks that are sufficient for exact sparse signal reconstruction. This number depends on two properties named intra- and inter-support block coherence. We then show that our bounds coincide with the best so far results in a series of examples, including Gaussian measurements or isolated measurements. We also show that the result is sharp when used with specific blocks in time-frequency bases, in the sense that the minimum required amount of blocks to reconstruct sparse signals cannot be improved up to a multiplicative logarithmic factor. The proposed results provide a good insight on the possibilities and limits of block compressed sensing in imaging devices, such as magnetic resonance imaging, radio-interferometry, or ultra-sound imaging.
Published: 2016

38. Interpenetrated Si-HPMC/alginate hydrogels as a potential scaffold for human tissue regeneration

Author: Lazhar Benyahia, Cécile Boyer, Gildas Rethore, Alexia Viguier, Taco Nicolai, Pierre Weiss, Christophe Chassenieux, Jérôme Guicheux, Institut des Molécules et Matériaux du Mans (IMMM), Le Mans Université (UM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre hospitalier universitaire de Nantes (CHU Nantes), This work has been funded by the Agence Nationale de la Recherche in the framework of ANR-11-BSV5-0022., ANR-11-BSV5-0022,HYCAR,Hydrogels nano-renforcés pour l'ingénierie tissulaire et la médecine régénérative du cartilage(2011), Jehan, Frederic, and BLANC - Hydrogels nano-renforcés pour l'ingénierie tissulaire et la médecine régénérative du cartilage - - HYCAR2011 - ANR-11-BSV5-0022 - BLANC - VALID
Subjects: Time Factors, Biocompatible Materials, 02 engineering and technology, 01 natural sciences, SW1353 Cell, chemistry.chemical_compound, Hypromellose Derivatives, Glucuronic Acid, Materials Testing, Linear Response Regime, chemistry.chemical_classification, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Tissue Scaffolds, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Hexuronic Acids, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Hydrogels, Polymer, Silanes, 021001 nanoscience & nanotechnology, 6. Clean water, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Self-healing hydrogels, Alginate Solution, Swelling, medicine.symptom, 0210 nano-technology, Materials science, Alginates, Cell Survival, Biomedical Engineering, Biophysics, chemistry.chemical_element, Bioengineering, Calcium, 010402 general chemistry, Polysaccharide, Cell Line, Biomaterials, medicine, Humans, Syneresis, Guided Tissue Regeneration, Alginate, Interpenetrate Polymer Network, technology, industry, and agriculture, Water, Glucuronic acid, 0104 chemical sciences, body regions, chemistry, Chemical engineering, Methyl cellulose
Abstract: International audience; Interpenetrated gels of biocompatible polysaccharides alginate and silanized hydroxypropyl methyl cellulose (Si-HPMC) have been studied in order to assess their potential as scaffolds for the regeneration of human tissues. Si-HPMC networks were formed by reduction of the pH to neutral and alginate networks were formed by progressive in situ release of Ca+. Linear and non-linear mechanical properties of the mixed gels at different polymer and calcium concentrations were compared with those of the corresponding single gels. The alginate/Si-HPMC gels were found to be stiffer than pure Si-HPMC gels, but weaker and more deformable than pure alginate gels. No significant difference was found for the maximum stress at rupture measured during compression for all these gels. The degrees of swelling or contraction in excess water at pH 7 as well as the release of Ca2+ was measured as a function of time. Pure alginate gels contracted by as much as 50 % and showed syneresis, which was much reduced or even eliminated for mixed gels. The important release of Ca2+ upon ageing for pure alginate gels was much reduced for the mixed gels. Furthermore, results of cytocompatibility assays indicated that there was no cytotoxicity of Si- HPMC/alginate hydrogels in 2D and 3D culture of human SW1353 cells. The results show that using interpenetrated Si-HPMC/alginate gels has clear advantages over the use of single gels for application in tissue regeneration.
Published: 2016

39. TGF-β1 and GDF5 Act Synergistically to Drive the Differentiation of Human Adipose Stromal Cells toward Nucleus Pulposus-like Cells

Author: Julie Lesoeur, Pierre Weiss, Anne Camus, Laurent Lescaudron, Jérôme Guicheux, Maëva Ruel, Gaëlle Bonin, Borhane-Hakim Fellah, Anne Moreau, Cécile Boyer, Pauline Colombier, Johann Clouet, Université de Nantes - UFR Odontologie, Université de Nantes (UN), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, PHU 11 Pharmacie [CHU Nantes] (Pharmacie Centrale), Centre hospitalier universitaire de Nantes (CHU Nantes), Service d'Anatomopathologie [Nantes], Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes] (Pôle hospitalo-universitaire PHU4 - OTONN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), This work was supported by INSERM and grants from the Fondation pour la Recherche Médicale (FRM pionnier de la recherche 'Bioingénierie'), the AO Foundation (S-12-14G), the Fondation de l’Avenir pour la Recherche Médicale Appliquée (FARMA ET3-683), the région Pays de la Loire (BIOREGOS II and BIODIV 'nouvelle équipe-nouvelle thématique' program), the Agence Nationale pour la Recherche (ANR générique 2014 REMEDIV), and doctoral grant from the AXA Research Fund (to P.C.)., Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Institut de Mathématiques de Toulouse UMR5219 (IMT), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Université de Nantes - UFR Odontologie (UFR Odonto), Université de Rennes (UR), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Jehan, Frederic
Subjects: 0301 basic medicine, Stromal cell, Nucleus Pulposus, Cellular differentiation, [SDV]Life Sciences [q-bio], Smad-driven nucleopulpogenic differentiation, Smad Proteins, SMAD, Intervertebral Disc Degeneration, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Stem cells, Biology, Cell morphology, Mesenchymal Stem Cell Transplantation, Regenerative medicine, Transforming Growth Factor beta1, 03 medical and health sciences, Mice, Growth Differentiation Factor 5, Adipocytes, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Animals, Humans, Cell Engineering, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Mesenchymal stem cell, Growth differentiation factor, Cell Differentiation, Mesenchymal Stem Cells, Cell Biology, Anatomy, Intervertebral disc, Cell biology, Extracellular Matrix, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, 030104 developmental biology, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Molecular Medicine, Stem cell, Low Back Pain, Developmental Biology
Abstract: Degenerative disc disease (DDD) primarily affects the central part of the intervertebral disc namely the nucleus pulposus (NP). DDD explains about 40% of low back pain and is characterized by massive cellular alterations that ultimately result in the disappearance of resident NP cells. Thus, repopulating the NP with regenerative cells is a promising therapeutic approach and remains a great challenge. The objectives of this study were to evaluate the potential of growth factor-driven protocols to commit human adipose stromal cells (hASCs) toward NP-like cell phenotype and the involvement of Smad proteins in this differentiation process. Here, we demonstrate that the transforming growth factor-β1 and the growth differentiation factor 5 synergistically drive the nucleopulpogenic differentiation process. The commitment of the hASCs was robust and highly specific as attested by the expression of NP-related genes characteristic of young healthy human NP cells. In addition, the engineered NP-like cells secreted an abundant aggrecan and type II collagen rich extracellular matrix comparable with that of native NP. Furthermore, we demonstrate that these in vitro engineered cells survived, maintained their specialized phenotype and secretory activity after in vivo transplantation in nude mice subcutis. Finally, we provide evidence suggesting that the Smad 2/3 pathway mainly governed the acquisition of the NP cell molecular identity while the Smad1/5/8 pathway controlled the NP cell morphology. This study offers valuable insights for the development of biologically-inspired treatments for DDD by generating adapted and exhaustively characterized autologous regenerative cells.
Published: 2016

40. Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell differently support bone repair in rats

Author: Sophie Frasca, Catherine Le Visage, Elhadi Sari Ali, Jean-Marc Collombet, Xavier Holy, Didier Letourneur, Françoise Norol, Institut de Recherche Biomédicale des Armées (IRBA), Service de biothérapies [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Departement Hospitalo- Universitaire - Inflammation, Immunopathologie, Biothérapie [Paris] (DHU - I2B), Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Jehan, Frederic, Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-CHU Saint-Antoine [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP]
Subjects: 0301 basic medicine, Calcium Phosphates, Male, Ceramics, Materials science, Bone Regeneration, Tissue Engineering Constructs and Cell Substrates, Biomedical Engineering, Biophysics, Bioengineering, Biocompatible Materials, Bone Marrow Cells, 02 engineering and technology, Bone healing, Bone resorption, Biomaterials, 03 medical and health sciences, Tissue engineering, Polysaccharides, Animals, Femur, Bone Resorption, Bone regeneration, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Bone Transplantation, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Neovascularization, Pathologic, Tissue Engineering, Tissue Scaffolds, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Mesenchymal stem cell, Hydrogels, Mesenchymal Stem Cells, X-Ray Microtomography, 021001 nanoscience & nanotechnology, Resorption, Rats, 030104 developmental biology, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Rats, Inbred Lew, Self-healing hydrogels, Chemical Engineering(all), Stem cell, 0210 nano-technology, Biomedical engineering
Abstract: International audience; Research in bone tissue engineering is focused on the development of alternatives to autologous bone grafts for bone reconstruction. Although multiple stem cell-based products and biomaterials are currently being investigated, comparative studies are rarely achieved to evaluate the most appropriate approach in this context. Here, we aimed to compare different clinically relevant bone tissue engineering methods and evaluated the kinetic repair and the bone healing efficiency supported by mesenchymal stem cells and two different biomaterials, a new hydrogel scaffold and a commercial hydroxyapatite/tricalcium phosphate ceramic, alone or in combination. Syngeneic mesenchymal stem cells (5 × 10 5) and mac-roporous biphasic calcium phosphate ceramic granules (Calciresorb C35 ® , Ceraver) or porous pullulan/dextran-based hydrogel scaffold were implanted alone or combined in a drilled-hole bone defect in rats. Using quantitative microtomography measurements and qualitative histologi-cal examinations, their osteogenic properties were evaluated 7, 30, and 90 days after implantation. Three months after surgery, only minimal repair was evidenced in control rats while newly mineralized bone was massively observed in animals treated with either hydrogels (bone volume/tissue volume = 20%) or ceramics (bone volume/tissue volume = 26%). Repair mechanism and resorption kinetics were strikingly different: rapidly-resorbed hydrogels induced a dense bone mineralization from the edges of the defect while ceramics triggered newly woven bone formation in close contact with the ceramic surface that remained unre-sorbed. Delivery of mesenchymal stem cells in combination with these biomaterials enhanced both bone healing (>20%) and neovascularization after 1 month, mainly in hydrogel. Osteogenic and angiogenic properties combined with rapid resorption make hydrogels a promising alternative to ceramics for bone repair by cell therapy.
Published: 2016

41. Longitudinal Comparison of Enzyme- and Laser-Treated Intervertebral Disc by MRI, X-Ray, and Histological Analyses Reveals Discrepancies in the Progression of Disc Degeneration: A Rabbit Study

Author: Marion Fusellier, Johann Clouet, Stéphane Madec, Olivier Hamel, Jérôme Guicheux, Olivier Gauthier, Pauline Colombier, Julie Lesoeur, Samy Youl, Groupe STEP (Inserm U791 - LIOAD), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), centre de Recherche et d'Investigation Préclinique [Nantes] (CRIP), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes] (Pôle hospitalo-universitaire PHU4 - OTONN), Centre hospitalier universitaire de Nantes (CHU Nantes), Unité de Pharmacie [CHU Nantes], Faculté de Pharmacie - Université de Nantes, This study was supported by grants fromSociété Française de Rhumatologie, Société Française de Neurochirurgie, Agence Nationale de la Recherche AAP TeCSAN (Chondrograft project), Agence de la Biomédecine, Institut National de la Santé Et de la Recherche Médicale (INSERM), Fondation pour la Recherche Médicale-MESCLE (FRM-MESCLE), Région des Pays de la Loire (BIOREGOS project, Gérontopôle LMA), ANR génériques 2013 (REMEDIV project), FRM Bioingénierie (DBS20131128442), and theUniversityHospital of Nantes, LMA., Jehan, Frederic, and École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)
Subjects: musculoskeletal diseases, Pathology, medicine.medical_specialty, Nucleus Pulposus, Article Subject, Radiography, 0206 medical engineering, lcsh:Medicine, Intervertebral Disc Degeneration, 02 engineering and technology, Degeneration (medical), Regenerative medicine, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Degenerative disease, In vivo, medicine, Animals, Regeneration, Longitudinal Studies, Laser beams, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, General Immunology and Microbiology, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, business.industry, Lasers, X-Rays, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Intervertebral Disc Chemolysis, lcsh:R, Intervertebral disc, General Medicine, medicine.disease, musculoskeletal system, Magnetic Resonance Imaging, 020601 biomedical engineering, 3. Good health, Disease Models, Animal, medicine.anatomical_structure, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Disc degeneration, Disease Progression, Female, Rabbits, business, 030217 neurology & neurosurgery, Research Article
Abstract: International audience; Regenerative medicine is considered an attractive prospect for the treatment of intervertebral disc (IVD) degeneration. To assess the efficacy of the regenerative approach, animal models of IVD degeneration are needed. Among these animal models, chemonucleolysis based on the enzymatic degradation of the Nucleus Pulposus (NP) is often used, but this technique remains far from the natural physiopathological process of IVD degeneration. Recently, we developed an innovative animal model of IVD degeneration based on the use of a laser beam. In the present study, this laser model was compared with the chemonucleolysis model in a longitudinal study in rabbits. The effects of the treatments were studied by MRI (T2-weighted signal intensity (T2wsi)), radiography (IVD height index), and histology (NP area and Boos' scoring). The results showed that both treatments induced a degeneration of the IVD with a decrease in IVD height and T2wsi as well as NP area and an increase in Boos' scoring. The enzyme treatment leads to a rapid and acute process of IVD degeneration. Conversely, laser radiation induced more progressive and less pronounced degeneration. It can be concluded that laser treatment provides an instrumental in vivo model of slowly evolving IVD degenerative disease that can be of preclinical relevance for assessing new prophylactic biological treatments of disc degeneration.
Published: 2016

42. Prediction of hamstring injury in professional soccer players by isokinetic measurements

Author: C. Dubois, Alban Fouasson-Chailloux, Pierre Menu, Marc Dauty, Sophie Ferreol, Jehan, Frederic, Groupe STEP (Inserm U791 - LIOAD), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Nantes - Pôle médecine physique et de réadaptation (CHU Nantes - PHU/MPR), and Centre hospitalier universitaire de Nantes (CHU Nantes)-Hôpital Saint-Jacques [CHU Nantes]
Subjects: medicine.medical_specialty, Isokinetic strength, Concentric, 03 medical and health sciences, muscle contraction, 0302 clinical medicine, Physical medicine and rehabilitation, medicine, Eccentric, Orthopedics and Sports Medicine, Hamstring injury, 030222 orthopedics, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, business.industry, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, 030229 sport sciences, medicine.disease, eccentric, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Original Article, sports, business, strength, human activities, Hamstring
Abstract: International audience; Objectives: previous studies investigating the ability of isokinetic strength ratios to predict hamstring injuries in soccer players have reported conflicting results. Hypothesis: to determine if isokinetic ratios are able to predict hamstring injury occurring during the season in professional soccer players. Study Design: case-control study; Level of evidence: 3. Methods: from 2001 to 2011, 350 isokinetic tests were performed in 136 professional soccer players at the beginning of the soccer season. Fifty-seven players suffered hamstring injury during the season that followed the isokinetic tests. These players were compared with the 79 uninjured players. The bilateral concentric ratio (hamstring-to-hamstring), ipsilateral concentric ratio (hamstring-to-quadriceps), and mixed ratio (eccentric/concentric hamstring-to-quadriceps) were studied. The predic-tive ability of each ratio was established based on the likelihood ratio and post-test probability. Results: the mixed ratio (30 eccentric/240 concentric hamstring-to-quadriceps)
Published: 2016

43. Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption

Author: Jérôme Guicheux, Yohann Wittrant, Jérôme Abadie, Vincent Hivernaud, G. Nourissat, Stéphanie Krisa, Martial Masson, Laurent Beck, Christophe Merceron, Nelly Urban, Xavier Houard, Olivier Gauthier, Tristan Richard, Claire Vinatier, Julie Lesoeur, Elsa Mével, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Union Grap'Sud, Groupe d'Etude des Substances Végétales à Activité Biologique (GESVAB - EA 3675), Université Bordeaux Segalen - Bordeaux 2, Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Animaux modèles pour la recherche en oncologie comparée (AMaROC), Centre de Recherche Saint-Antoine (UMRS893), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, UMR791, Laboratoire d’Ingénierie Ostéo-Articulaire et Dentaire, OTONN PHU4 CHU de Nantes, Institut National de la Santé et de la Recherche Médicale (INSERM), Animaux Mode' les pour la Recherche en Oncologie (AMaROC), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), and HAL UPMC, Gestionnaire
Subjects: Male, 0301 basic medicine, Interleukin-1beta, prévention des maladies, Administration, Oral, Nitric Oxide Synthase Type II, Pharmacology, Catechin, Mass Spectrometry, chemistry.chemical_compound, 0302 clinical medicine, Oral administration, arthrose, Medicine, Anterior Cruciate Ligament, Multidisciplinary, Phenylethyl Alcohol, surgical procedures, operative, Proanthocyanidin, Grape seed extract, Alimentation et Nutrition, Metabolome, Female, Rabbits, [SDV.SP.MED] Life Sciences [q-bio]/Pharmaceutical sciences/Medication, food.ingredient, Nitric Oxide, Dinoprostone, Article, Nitric oxide, effet de l'âge, polyphénol, 03 medical and health sciences, food, [SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication, Olea, Matrix Metalloproteinase 13, Osteoarthritis, Animals, Biflavonoids, Food and Nutrition, Proanthocyanidins, RNA, Messenger, cardiovascular diseases, 030203 arthritis & rheumatology, Grape Seed Extract, business.industry, In vitro, Diet, Bioavailability, Mice, Inbred C57BL, Disease Models, Animal, 030104 developmental biology, chemistry, Cyclooxygenase 2, Wounds and Injuries, Hydroxytyrosol, business, rôle protecteur
Abstract: The authors are grateful to B. Fellah, I. Leborgne, and P. Roy (CRIP-ONIRIS) who contributed to experiments using rabbits; S. Sourice (Inserm UMRS 791) for her advice with histological analysis; E. Hay (Inserm UMRS 1132) for his advice with NITEGE IHC; P.Pilet for his fruitful discussion for IHC intensity analysis; F. Berenbaum for his help in DMM mice model; UTE Platform (SFR Francois Bonamy, FED 4203/ Inserm UMS 016/CNRS 3556) who provided daily care to animals and specially P. Monmousseau who provided help with forced-fed rabbits; MS and NMR experiments were undertaken at the Metabolome Facility at the Functional Genomic Center in Bordeaux, France and specially E. Renouf for her assistance in UPLC-MS analysis. R Pace who provided help with manuscript. This study was supported by Grap'Sud, Region Languedoc Roussillon, the BPI and the ANRT; International audience; Polyphenols exert a large range of beneficial effects in the prevention of age-related diseases. We sought to determine whether an extract of olive and grape seed standardized according to hydroxytyrosol (HT) and procyanidins (PCy) content, exerts preventive anti-osteoathritic effects. To this aim, we evaluated whether the HT/PCy mix could (i) have in vitro anti-inflammatory and chondroprotective actions, (ii) exert anti-osteoarthritis effects in two post-traumatic animal models and (iii) retain its bioactivity after oral administration. Anti-inflammatory and chondroprotective actions of HT/PCy were tested on primary cultured rabbit chondrocytes stimulated by interleukin-1 beta (IL-1β). The results showed that HT/PCy exerts anti-inflammatory and chondroprotective actions in vitro. The preventive effect of HT/PCy association was assessed in two animal models of post-traumatic OA in mice and rabbits. Diet supplementation with HT/PCy significantly decreased the severity of post-traumatic osteoarthritis in two complementary mice and rabbit models. The bioavailability and bioactivity was evaluated following gavage with HT/PCy in rabbits. Regular metabolites from HT/PCy extract were found in sera from rabbits following oral intake. Finally, sera from rabbits force-fed with HT/PCy conserved anti-IL-1β effect, suggesting the bioactivity of this extract. To conclude, HT/PCy extract may be of clinical significance for the preventive treatment of osteoarthritis.
Published: 2016

44. Functional Langerin high -Expressing Langerhans-like Cells Can Arise from CD14 high CD16 − Human Blood Monocytes in Serum-Free Condition

Author: Régis Josien, Coraline Chéneau, Fabienne Haspot, Jerome Martin, Michèle Heslan, Jean-Marc Humbert, Gaelle Picarda, Franck Halary, Franck Duteille, Gaelle Beriou, P. Pilet, Frédérique Bellier-Waast, Fabien Guillon, Pierre Perrot, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Faculté de Médecine - Université de Nantes, La Jolla Institute for Immunology [La Jolla, CA, États-Unis], Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Chirurgie Plastique et Reconstructive [Nantes], Clinique Bretéché [Nantes], The Immunobiothérapies et Cellules Dendritiques and Alliance pour la Recherche sur les Maladies Infectieuses Nantes Angers consortia and the Région Pays de Loire Modèle Organotypique de Muqueuse Humaine Immunocompétente project. The Institut Hospitalo-Universitaire-Cesti project is also supported by Nantes Métropole and Région Pays de la Loire., ANR: ANR-11LABX-0016-01, ANR: ANR-10IBHU-005, ANR-11-LABX-0016,IGO,Immunothérapies Grand Ouest(2011), ANR-10-IBHU-0005,CESTI (TSI-IHU),Centre Européen des Sciences de la Transplantation et de l'Immunothérapie (TSI-IHU)(2010), Le Bihan, Sylvie, Laboratoires d'excellence - Immunothérapies Grand Ouest - - IGO2011 - ANR-11-LABX-0016 - LABX - VALID, and Instituts Hospitalo-Universitaires B - Centre Européen des Sciences de la Transplantation et de l'Immunothérapie (TSI-IHU) - - CESTI (TSI-IHU)2010 - ANR-10-IBHU-0005 - IBHU - VALID
Subjects: 0301 basic medicine, Keratinocytes, Chemokine, Isoantigens, Langerin, Birbeck granules, Ultraviolet Rays, Cellular differentiation, T cell, T-Lymphocytes, Immunology, Lipopolysaccharide Receptors, Lymphocyte Activation, Monocytes, Transforming Growth Factor beta1, 03 medical and health sciences, Immune system, Antigens, Neoplasm, Proto-Oncogene Proteins, medicine, Immunology and Allergy, Humans, Cells, Cultured, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Blood Cells, biology, integumentary system, Receptors, IgG, Peripheral tolerance, Granulocyte-Macrophage Colony-Stimulating Factor, Receptor Protein-Tyrosine Kinases, Cell Differentiation, Axl Receptor Tyrosine Kinase, Cell biology, CCL20, 030104 developmental biology, medicine.anatomical_structure, Self Tolerance, Langerhans Cells, biology.protein, Cell Adhesion Molecules, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Langerhans cells (LCs) are epithelial APCs that sense danger signals and in turn trigger specific immune responses. In steady-state, they participate in the maintenance of peripheral tolerance to self-antigens whereas under inflammation LCs efficiently trigger immune responses in secondary lymphoid organs. It has been demonstrated in mice that LC-deprived epithelia are rapidly replenished by short half-life langerin-expressing monocyte-derived LCs (MDLCs). These surrogate LCs are thought to be progressively replaced by langerinhigh LCs arising from self-renewing epithelial precursors of hematopoietic origin. How LCs arise from blood monocytes is not fully understood. Hence, we sought to characterize key factors that induce differentiation of langerinhigh-expressing monocyte-derived Langerhans-like cells. We identified GM-CSF and TGF-β1 as key cytokines to generate langerinhigh-expressing cells but only in serum-free conditions. These cells were shown to express the LC-specific TROP-2 and Axl surface markers and contained Birbeck granules. Surprisingly, E-cadherin was not spontaneously expressed by these cells but required a direct contact with keratinocytes to be stably induced. MDLCs induced stronger allogeneic T cell proliferations but released low amounts of inflammatory cytokines upon TLR stimulation compared with donor-paired monocyte-derived dendritic cells. Immature langerinhigh MDLCs were responsive to MIP-3β/CCL20 and CTAC/CCL27 chemokine stimulations. Finally, we demonstrated that those cells behaved as bona fide LCs when inserted in a three-dimensional rebuilt epithelium by becoming activated upon TLR or UV light stimulations. Collectively, these results prompt us to propose these langerinhigh MDLCs as a relevant model to address LC biology–related questions.
Published: 2016

45. A simple and effective approach to prepare injectable macroporous calcium phosphate cement for bone repair: Syringe-foaming using a viscous hydrophilic polymeric solution

Author: Pierre Weiss, Weizhen Liu, Sophie Sourice, P. Pilet, Olivier Gauthier, Jean-Michel Bouler, Khalid Khairoun, Gildas Rethore, Franck Tancret, Jingtao Zhang, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Matériaux d'intérêt biologique, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Matériaux Jean Rouxel (IMN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN), BASCHERA, Richard, Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN), and Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Calcium Phosphates, Compressive Strength, Polymers, Biocompatible Materials, 02 engineering and technology, 01 natural sciences, Biochemistry, Hypromellose Derivatives, Materials Testing, Composite material, chemistry.chemical_classification, Viscosity, Bone Cements, Si-HPMC hydrogel, General Medicine, Polymer, 021001 nanoscience & nanotechnology, Calcium phosphate cements, Bone regeneration, medicine.anatomical_structure, Compressive strength, Rabbits, Powders, 0210 nano-technology, Porosity, Cancellous bone, Macropores, Biotechnology, [CHIM.POLY] Chemical Sciences/Polymers, Materials science, Biomedical Engineering, Foaming agent, Bone healing, 010402 general chemistry, Biomaterials, medicine, Animals, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Molecular Biology, Syringes, Silated polymer, technology, industry, and agriculture, 0104 chemical sciences, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, [CHIM.POLY]Chemical Sciences/Polymers, chemistry, Bone Substitutes, Stress, Mechanical
Abstract: In this study, we propose a simple and effective strategy to prepare injectable macroporous calcium phosphate cements (CPCs) by syringe-foaming via hydrophilic viscous polymeric solution, such as using silanized-hydroxypropyl methylcellulose (Si-HPMC) as a foaming agent. The Si-HPMC foamed CPCs demonstrate excellent handling properties such as injectability and cohesion. After hardening the foamed CPCs possess hierarchical macropores and their mechanical properties (Young's modulus and compressive strength) are comparable to those of cancellous bone. Moreover, a preliminary in vivo study in the distal femoral sites of rabbits was conducted to evaluate the biofunctionality of this injectable macroporous CPC. The evidence of newly formed bone in the central zone of implantation site indicates the feasibility and effectiveness of this foaming strategy that will have to be optimized by further extensive animal experiments.A major challenge in the design of biomaterial-based injectable bone substitutes is the development of cohesive, macroporous and self-setting calcium phosphate cement (CPC) that enables rapid cell invasion with adequate initial mechanical properties without the use of complex processing and additives. Thus, we propose a simple and effective strategy to prepare injectable macroporous CPCs through syringe-foaming using a hydrophilic viscous polymeric solution (silanized-hydroxypropyl methylcellulose, Si-HPMC) as a foaming agent, that simultaneously meets all the aforementioned aims. Evidence from our in vivo studies shows the existence of newly formed bone within the implantation site, indicating the feasibility and effectiveness of this foaming strategy, which could be used in various CPC systems using other hydrophilic viscous polymeric solutions.
Published: 2016

46. A new injectable radiopaque chitosan-based sclerosing embolizing hydrogel for endovascular therapies

Author: Ahmed Fatimi, Jean-Michel Coutu, Sophie Lerouge, S. Berrahmoune, Gilles Soulez, Pascal Chabrot, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Image Science for Interventional Techniques (ISIT), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Centre National de la Recherche Scientifique (CNRS), Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), CHU Clermont-Ferrand, Ecole de Technologie Supérieure [Montréal] (ETS), and SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])
Subjects: Time Factors, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, medicine.medical_treatment, Contrast Media, 02 engineering and technology, Biochemistry, Endovascular aneurysm repair, Hydrogel, Polyethylene Glycol Dimethacrylate, 030218 nuclear medicine & medical imaging, Sodium Tetradecyl Sulfate, Chitosan, chemistry.chemical_compound, 0302 clinical medicine, Iliac Aneurysm, Embolization, ComputingMilieux_MISCELLANEOUS, Endovascular Procedures, General Medicine, Hydrogen-Ion Concentration, 021001 nanoscience & nanotechnology, Embolization, Therapeutic, Sodium tetradecyl sulfate, 3. Good health, Self-healing hydrogels, cardiovascular system, Radiology, Rheology, 0210 nano-technology, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Biotechnology, medicine.drug, medicine.medical_specialty, Materials science, Biomedical Engineering, macromolecular substances, In Vitro Techniques, Injections, Biomaterials, 03 medical and health sciences, Dogs, Aneurysm, Elastic Modulus, medicine, Animals, cardiovascular diseases, Molecular Biology, Factor VIII, technology, industry, and agriculture, Stent, medicine.disease, Sclerosing Solutions, Surgery, Disease Models, Animal, chemistry, Microscopy, Electron, Scanning
Abstract: Endovascular repair of abdominal aortic aneurysms with a stent graft is limited by the persistence or recurrence of endoleaks. These are believed to be related to the recanalization of the aneurismal sac by endothelialized neochannels, which could lead to late type I and II endoleaks. Embolization has been proposed to treat or prevent endoleaks, but presently commercialized embolizing materials have several drawbacks and do not fully prevent endoleak recurrence. A novel chitosan hydrogel that is injectable, radiopaque and contains sodium tetradecyl sulfate (STS), a well-known sclerosing agent, was developed in order to combine blood flow occlusion and endothelium ablation properties. chitosan/STS hydrogels were characterized and optimized using rheometry, scanning electron microscopy, swelling and ex vivo embolization assay. They were shown to exhibit rapid gelation and good mechanical properties, as well as sclerosing properties. Their potential for the embolization of aneurysms was subjected to preliminary in vivo evaluation in a bilateral iliac aneurysm model (three dogs) reproducing persistent endoleaks after endovascular aneurysm repair (EVAR). At 3 months no endoleak was detected in any of the three aneurysms treated with chitosan/STS hydrogels. In contrast, type I endoleaks were detected in two of the three aneurysms treated with chitosan hydrogels. Generally, chitosan/STS hydrogels have great potential as embolizing and sclerosing agents for EVAR and possibly other endovascular therapies.
Published: 2012

47. Tissue Engineering and Regenerative Medicine

Author: Geoff Dumsday, Leping Yan, Hans Leemhuis, Sandra Vieira, Tiago Pereira, Veronica Glattauer, Laurent DAVID, Cristian Pablo Pennisi, James Gardiner, Francesca Pagliari, Arlyng Gyveth González Vázquez, Salvatrice Rigogliuso, Isabel Amaral, Rui Costa, Célia Henriques, Ana Cristina Rodrigues, Vladimir Zachar, Stefania Pagliari, Paulo Rui Fernandes, Alexandre Felipe Leitão, Morten Andersen, Carlota García-Santiago, Clara Sanz Nogués, Meir Israelowitz, Frank Boury, Frederik Maes, Ana Rita Ferreira, Ferry Melchels, Clemens Holzer, João Paulo Borges, Ling Qin, Julio Suay, Catarina Santos, Vincenzo La Carrubba, YI LI, Emanuel Fernandes, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Micro et Nanomédecines Biomimétiques (MINT), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL), Institut des Molécules et Matériaux du Mans (IMMM), Le Mans Université (UM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Univ Angers, Okina
Subjects: [SDV]Life Sciences [q-bio], Niche, Biomedical Engineering, Medicine (miscellaneous), 02 engineering and technology, Biology, 021001 nanoscience & nanotechnology, Spinal cord, Biomaterials, [SDV] Life Sciences [q-bio], 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, medicine, 0210 nano-technology, Neuroscience, ComputingMilieux_MISCELLANEOUS, Progenitor
Abstract: International audience
Published: 2012
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48. Hydrogel/calcium phosphate composites require specific properties for three-dimensional culture of human bone mesenchymal cells

Author: Pierre Layrolle, Pierre Weiss, Pierre Corre, Jérôme Sohier, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de chirurgie maxillo-faciale [CHRU Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)
Subjects: Calcium Phosphates, Materials science, Cell Survival, Biomedical Engineering, Human bone, chemistry.chemical_element, Biocompatible Materials, 02 engineering and technology, Calcium, Matrix (biology), Biochemistry, Bone and Bones, Hydrogel, Polyethylene Glycol Dimethacrylate, Tissue Culture Techniques, Biomaterials, 03 medical and health sciences, Bioreactors, Materials Testing, Humans, Cell Lineage, Viability assay, Composite material, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Cell Proliferation, 030304 developmental biology, 0303 health sciences, Staining and Labeling, Tissue Scaffolds, Mesenchymal stem cell, Mesenchymal Stem Cells, General Medicine, Alkaline Phosphatase, 021001 nanoscience & nanotechnology, Biocompatible material, chemistry, Self-healing hydrogels, Stem cell, 0210 nano-technology, Biotechnology
Abstract: To provide multipotent cells with a three-dimensional environment closer to bone matrix, an engineered construct mimicking bone components has been designed and evaluated. A biocompatible hydrogel (silated hydroxypropylmethyl cellulose) was used as an extra-cellular matrix while biphasic calcium phosphate ceramic particles were used to replace mineralized matrix. Finally, human bone mesenchymal cells were cultured in three dimensions in the resulting constructs to study their cell viability, proliferation, interactions within the composites, and maintenance of their osteogenic potential. This approach resulted in homogeneous structures in which cells were viable and retained their osteoblastic differentiation potential. However, the cells did not proliferate nor colonize the constructs, possibly because of a lack of suitable interactions with their micro-environment.
Published: 2010

49. Differential effects of hypoxia on osteochondrogenic potential of human adipose-derived stem cells

Author: Pierre Weiss, Lydie Guigand, Sophie Portron, Jérôme Amiaud, Claire Vinatier, Yan Cherel, Christophe Merceron, Martial Masson, Jérôme Guicheux, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), UFR Odontologie, Université Paris Diderot - Paris 7 (UPD7), Graftys SA, Développement et Pathologie du Tissu Musculaire (DPTM), and Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes
Subjects: Genetic Markers, medicine.medical_specialty, Time Factors, OSTEOCHONDROGENESIS, Physiology, [SDV]Life Sciences [q-bio], Cellular differentiation, Osteocalcin, Core Binding Factor Alpha 1 Subunit, 03 medical and health sciences, Calcification, Physiologic, 0302 clinical medicine, Tissue engineering, Osteogenesis, Internal medicine, medicine, Humans, Aggrecans, Collagen Type II, Cells, Cultured, Aggrecan, Cell Proliferation, Glycosaminoglycans, 030304 developmental biology, 0303 health sciences, biology, Stem Cells, Cell Biology, Alkaline Phosphatase, Chondrogenesis, Cell Hypoxia, Oxygen tension, Cell biology, Oxygen, RUNX2, Phenotype, Endocrinology, Adipose Tissue, Gene Expression Regulation, 030220 oncology & carcinogenesis, biology.protein, Stem cell, ADIPOSE TISSUE-DERIVED STEM CELLS, Biomarkers
Abstract: International audience; Human adipose tissue-derived stem cells (hATSC) have been contemplated as reparative cells for cartilage engineering. Chondrogenic differentiation of hATSC can be induced by an enriched culture medium and a three-dimensional environment. Given that bone is vascularized and cartilage is not, oxygen tension has been suggested as a regulatory factor for osteochondrogenic differentiation. Our work aimed at determining whether hypoxia affects the osteochondrogenic potential of hATSC. hATSC were cultured in chondrogenic or osteogenic medium for 28 days, in pellets or monolayers, and under 5% or 20% oxygen tension. Cell differentiation was monitored by real-time PCR (COL2A1, aggrecan, Runx2, and osteocalcin). The chondrogenic differentiation was further evaluated by Alcian blue and immunohistological staining for glycosaminoglycans (GAGs) and type II collagen, respectively. Osteogenic differentiation was also assessed by the staining of mineralized matrix (Alizarin Red) and measurement of alkaline phosphatase (ALP) activity. The expression of chondrogenic markers was upregulated when hATSC were exposed to hypoxia in chondrogenic medium. Conversely, osteocalcin expression, mineralization, and ALP activity were severely reduced under hypoxic conditions even in the presence of osteogenic medium. Our data strongly suggest that hypoxia favors the chondrogenic differentiation of hATSC as evidenced by the expression of the chondrogenic markers, whereas it could alter their osteogenic potential. Our results highlight the differential regulatory role of hypoxia on the chondrogenic and osteogenic differentiation processes of hATSC. These data could help us exploit the potential of tissue engineering and stem cells to replace or restore the function of osteoarticular tissues.
Published: 2010

50. Osteogenicity of biphasic calcium phosphate ceramics and bone autograft in a goat model

Author: Daniel Chappard, Pierre Layrolle, Borhane H. Fellah, Pierre Weiss, Olivier Gauthier, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Chirurgie expérimentale (ENV), Ecole Nationale Vétérinaire de Nantes, Groupe d'Études Remodelage Osseux et bioMatériaux (GEROM), Université d'Angers (UA), Equipe de recherche clinique en Odontologie (ERT1051), and Université de Nantes (UN)
Subjects: Calcium Phosphates, Ceramics, [SDV]Life Sciences [q-bio], Dentistry, 02 engineering and technology, Osteoconduction, chemistry.chemical_compound, 0302 clinical medicine, Osteogenesis, Materials Testing, Femur, Polytetrafluoroethylene, Bone autograft, Bone Transplantation, Goats, Synthetic bone, 021001 nanoscience & nanotechnology, Biphasic calcium phosphate, Bone ingrowth, Mechanics of Materials, Female, Hydroxyapatites, 0210 nano-technology, Materials science, Biophysics, chemistry.chemical_element, Bioengineering, Calcium, Transplantation, Autologous, Biomaterials, 03 medical and health sciences, Osseointegration, Animals, Bone Resorption, Muscle, Skeletal, Tissue Engineering, business.industry, Calcium phosphate ceramics, 030206 dentistry, Autologous bone, Phosphate, Osteoinduction, chemistry, Bone Substitutes, Microscopy, Electron, Scanning, Ceramics and Composites, business, Biomedical engineering
Abstract: International audience; The aim of this work was to compare the osteogenicity of calcium phosphate ceramic granules with autologous bone graft in ectopic and orthotopic sites. Biphasic calcium phosphate (BCP) granules composed of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) in a 60/40 ratio were sintered at 1050, 1125 and 1200 °C, producing different microporosities. Either BCP ceramic granules or autologous bone chips (n = 7) were implanted into paraspinal muscles. Osteoinduction was not observed in either the BCP implants or autologous bone chips after 6 or 12 weeks in the ectopic sites. Hollow and bored polytetrafluoroethylene (PTFE) cylinders were filled with autologous bone, BCP granules or left empty, then implanted into critical-sized defects in femoral epiphyses. The PTFE cylinders left empty contained marrow and blood vessels but not mineralized bone, indicating that this model prevented bone ingrowth (0.56 ± 0.43% at 12 weeks). Bone formation was observed in contact with the BCP1050 and BCP1125 granules in the femoral sites after 6 weeks. The amount of bone after 12 weeks was 5.6 ± 7.3 and 9.6 ± 6.6% for BCP1050 and BCP1125, respectively. Very little bone formation was observed with the BCP1200 implants (1.5 ± 1.3% at 12 weeks). In both the ectopic and orthotopic sites, autologous bone chips were drastically resorbed (from 19.4 ± 3.7% initially to 1.7 ± 1.2% at 12 weeks). This study shows that synthetic bone substitutes may have superior stability and osteogenic properties than autologous bone grafts in critical-sized bone defects.
Published: 2008

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