1. Role of activation-induced cell death in pathogenesis of patients with chronic hepatitis B.
- Author
-
Hou CS, Wang GQ, Lu SL, Yue B, Li MR, Wang XY, and Yu JW
- Subjects
- Adult, Cells, Cultured, Cytokines metabolism, Female, Flow Cytometry, Hepatitis B, Chronic etiology, Humans, In Vitro Techniques, Male, Microscopy, Fluorescence, Middle Aged, T-Lymphocytes immunology, T-Lymphocytes metabolism, Cell Death, Hepatitis B, Chronic immunology, Hepatitis B, Chronic pathology, Lymphocyte Activation, T-Lymphocytes cytology
- Abstract
Aim: To study and compare the difference of activation-induced cell death (AICD) in peripheral blood T-lymphocytes(PBL-Ts) from patients with chronic hepatitis B (CHB) and the normal people in vitro, and to explore the role of AICD in chronic hepatitis B virus (HBV) infection and the pathogenesis of CHB., Methods: Twenty-five patients and fourteen healthy people were selected for isolation of PBL-Ts. During cultivation, anti-CD3 mAb, PMA and ionomycin were used for AICD of PBL-Ts. AICD ratio of PBL-Ts was detected with TdT-mediated dUTP nick end labeling and assessed by flow cytometry., Results: When induced with anti-CD3, PMA and ionomycin in vitro, AICD ratio of PBL-Ts from CHB patients was significantly higher than that from healthy control (17.24+/-1.21 vs. 6.63+/-1.00, P<0.01) and that from CHB patients without induction (17.24+/-1.21 vs. 9.88+/-1.36, P<0.01). There was a similar AICD ratio of PBL-Ts between induction group and without induction group, but no difference was found before and after induction in healthy control. The density of INF-gamma in culture media of induction groups of CHB was lower than that of other groups (P<0.01). There was no difference between these groups in density of IL-10 (P>0.05)., Conclusion: When induced during cultivation in vitro, PBL-Ts from CHB have AICD very commonly. This phenomenon has a potentially important relation with pathogenesis of CHB and chronicity of HBV infection.
- Published
- 2003
- Full Text
- View/download PDF