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Blockchain has become one of the key techniques for the security of the industrial internet. However, the blockchain is vulnerable to FAW (Fork after Withholding) attacks. To protect the industrial internet from FAW attacks, this paper proposes a novel FAW attack protection algorithm (FAWPA) based on the behavior of blockchain miners. Firstly, FAWPA performs miner data preprocessing based on the behavior of the miners. Then, FAWPA proposes a behavioral reward and punishment mechanism and a credit scoring model to obtain cumulative credit value with the processed data. Moreover, we propose a miner's credit classification mechanism based on fuzzy C-means (FCM), which combines the improved Aquila optimizer (AO) with strong solving ability. That is, FAWPA combines the miner's accumulated credit value and multiple attack features as the basis for classification, and optimizes cluster center selection by simulating Aquila's predation behavior. It can improve the solution update mechanism in different optimization stages. FAWPA can realize the rapid classification of miners' credit levels by improving the speed of identifying malicious miners. To evaluate the protective effect of the target mining pool, FAWPA finally establishes a mining pool and miner revenue model under FAW attack. The simulation results show that FAWPA can thoroughly and efficiently detect malicious miners in the target mining pool. FAWPA also improves the recall rate and precision rate of malicious miner detection, and it improves the cumulative revenue of the target mining pool. The proposed algorithm performs better than ND, RSCM, AWRS, and ICRDS. [ABSTRACT FROM AUTHOR]

Background: Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this study, its antiviral effect against herpes simplex virus (HSV) type 1 and 2 (HSV-1 and HSV-2) replication was investigated. Results: Wogonin suppressed HSV-2-induced cytopathic effect (CPE) and reduced viral mRNA transcription, viral protein synthesis, and infectious virion particle titers in a dose-dependent manner. A time-of-drug-addition assay demonstrated that wogonin acted as a postentry viral inhibitor. Wogonin also significantly reduced HSV-induced NF-κB and MAPK pathway activation, which has previously been demonstrated to be important for viral replication. Conclusions: Our results suggest that the anti-herpes effect of wogonin may be mediated by modulation of cellular NF-κB and JNK/p38 MAPK pathways and imply that wogonin may be useful as an anti-HSV agent. [ABSTRACT FROM AUTHOR]

Abstract: The scandal of melamine-adulterated infant formula in China in September 2008 demanded the need to assess the extent of melamine contamination in the environment and food products and possible risks of consuming melamine-contaminated diets. In this work, our extensive work tested water, soil and crop samples from 21 provinces in China. Soils nearby and waste waters from melamine-manufacturing factories were examined, and the highest melamine concentrations in waste water and soil samples were 226.766 and 41.136mg/kg, respectively. Six of 94 irrigation water samples had melamine at a concentration of 21–198μg/L. Only 1 sample collected from 124 farmlands farther than 150km from melamine factories was detected for melamine at a content of 176μg/L. Only 3 out of 557 crop samples contaminated more than 1mg/kg melamine, with the highest level of 2.05mg/kg in a wheat sample. When basal diets contained 2mg/kg melamine were fed to various animals, deposition of melamine in animal tissues and products was all lower than 122μg/kg. The melamine deposition was much higher (e.g., 4483μg/kg in the kidney of chicken) when diets contained 100mg/kg melamine but was found to be completely depleted after 96h for all animals after switching to the basal diets. Our work may be valuable to regulate melamine production and monitor the safety of food and animal products. [Copyright &y& Elsevier]

A new method for the determination of oxytetracycline (OTC) has been established by coupling the catalytic amplification reaction of copper nanoclusters (CuNCs) with the aptamer reaction. CuNCs prepared by a wet chemical method have the catalytic activity for the formation of gold nanoparticles (AuNPs) resulting from a HAuCl4-ethanol (En) reaction. The experimental results showed that OTC aptamer (Apt) can be adsorbed on the surface of CuNCs in a non-specific way, thus inhibiting its catalytic activity. When OTC was added to the solution, the OTC-Apt complex was generated by a specific reaction, which made the CuNCs desorb and restore their catalytic activity. With the increase of OTC, the recovery of the catalytic activity of CuNCs is strengthened, the reaction speed is accelerated, and the number of AuNPs is increased. The generated AuNPs exhibited surface enhanced Raman scattering (SERS) signals at 1615 cm−1 in the presence of Vitoria blue 4R (VB4R) molecular probes, and a resonance Rayleigh scattering (RRS) peak at 586 nm. There is a good linear relationship between the intensities of SERS, or RRS, and OTC concentration at the range of 37.5–300 ng/L or 37.5–225 ng/L, respectively. A new SERS and RRS assay for the determination of trace OTC based on the regulation of CuNCs catalysis was established. [ABSTRACT FROM AUTHOR]

Background: The pattern recognition receptors (PPRs) are the earliest phase of the host defense against pathogens in genital epithelium, and toll-like receptors (TLRs) are best characterized PPRs mediating innate immune responses. Herpes simplex virus type 2 (HSV-2), a member of herpesviridae family, causes one of the most prevalent sexually transmitted infection in the world. In this paper, we described that HSV-2 infection would induce activator protein 1 (AP-1) via TLR4-MyD88/TRIF pathway in human genital epithelial cell. Methods: TLRs expression profiles and changes was investigated in HSV-2-infected cells. The effect of TLR4-MyD88/TRIF on HSV-2-induced AP-1 activation and viral replication was also evaluated. The TLR4 translocation change was examined after viral infection. Finally, viral ICP0 effect on TLR4 signaling and TLR4-promoter regulation were primarily studied. Results: HSV-2-induced AP-1 activation was dependent on TLR4 and downstream adaptor molecules MyD88 and TRIF. And also, TLR4, MyD88 and TRIF was proved to affect HSV-2 replication. AP-1 activation would also be enhanced via overexpression of myeloid differentiation protein 2 (MD2), implicating that it might be a necessary accessory for TLR4 to sense HSV-2 infection. Protein quantification of cytoplasmic and membrane-associated TLR4 revealed that HSV-2 infection increased membrane-anchoring TLR4 level, but not cytoplasmic ones. Viral ICP0 could augment cellular AP-1, TLR4 promoter activation and TLR4 expression level. The specific inhibitor treatment and transcription factor binding site scanning in TLR4 promoter region showed that AP-1 activity was essential for TLR4-promoter activation. Conclusions: Taken together, HSV-2 infection could stimulate AP-1 activation via TLR4-MyD88/TRIF axis, and then feedback to up-regulate TLR4 expression in human genital epithelial cells. [ABSTRACT FROM AUTHOR]

In recent years, Hybrid Cellular Automata Method (HCAM) has been successfully applied to solve structural topology optimization problems. However, there was no report on HCAM research of three-dimensional composite structure composed of multiple materials and multiple bodies. Therefore, in this paper, three-dimensional non-cube cells of irregular size (such as tetrahedral cells with adaptive changes inside length) and Finite Element Method (FEM) are introduced to extend HCAM, which is better and more flexibly to fit complex geometric shapes. Furthermore, a better structure configuration of multi-material and multi-body structure is obtained. The typical example study showed that the proposed topology optimization method could effectively remove the redundant materials of multi-material and multi-body structure, and the optimized structure configuration could still meet the requirements of the original condition after geometric reconstructed. Thus it provided a reference for the intelligent design of other products. [ABSTRACT FROM AUTHOR]

Time series have broad usage in the wireless Internet of Things. This article proposes a nonlinear time series prediction algorithm based on the Small-World Scale-Free Network after the AIC-Optimized Subtractive Clustering Algorithm (AIC-DSCA-SSNET, AD-SSNET) to predict the nonlinear and unstable time series, which improves the prediction accuracy. The AD-SSNET is introduced as a reservoir based on the echo state network to improve the predictive capability of nonlinear time series, and combined with artificial intelligence method to construct the prediction model training samples. First, the optimal clustering scheme of randomly distributed neurons in the network is adaptively obtained by the AIC-DSCA, then the AD-SSNET is constructed according to the intra-cluster priority connection algorithm. Finally, the reservoir synaptic matrix is calculated according to the synaptic information. Experimental results show that the proposed nonlinear time series prediction algorithm extends the feasible range of spectral radii of the reservoir, improves the prediction accuracy of nonlinear time series, and has great significance to time series analysis in the era of wireless Internet of Things. [ABSTRACT FROM AUTHOR]

An analytical method for calculating the no-load magnetic field of external rotor permanent magnet brushless direct current motor (PMBLDCM) used as in-wheel motor of electric vehicle in the stator static coordinate and the rotor motion coordinate is presented in this paper. First, the analytic formulas of slotless permanent magnetic field in both coordinate systems are derived, respectively. Then, the complex relative permeance of external rotor PMBLDCM in both coordinate systems is calculated. Finally, the analytical solution of the no-load magnetic field in both coordinate systems is derived by applying the magnetic potential multiplied by the complex relative permeance. In this paper, a 46-pole-51-slot external rotor PMBLDCM is taken as an example, and the accuracy of the proposed analytical model is verified by the finite-element results. Based on the analytical model, the influences of the stator slotting effect on the no-load magnetic field of the external rotor and the inner stator are analyzed. The spatial order characteristics and frequency characteristics of the no-load magnetic field of the external rotor PMBLDCM in both coordinate systems are revealed, respectively. [ABSTRACT FROM AUTHOR]

• The fretting wear mechanisms of Inconel 718 alloys deposited by laser cladding in different temperature were analyzed. • In PSR, the wear damage mainly shows elastic–plastic deformation, fatigue wear and abrasive wear. • In MFR and GSR, the wear damage was mainl combined with fatigue wear, peeling, adhesive wear and oxidative wear. • High temperatures induces oxidation reactions during friction and slow down the wear of the material. Fretting tests were performed on an Inconel 718 superalloy against GCr15 steel in a range of 25 ℃ < T < 400 ℃ to investigate the wear mechanism of the laser cladding-deposited Inconel 718 superalloy over a wide temperature range. The results show that when the displacement amplitude was 15 μm, the fretting was running in a partial slip regime (PSR), and the wear damage mainly showed elastic–plastic deformation, fatigue wear and abrasive wear. When the displacement amplitude increased to 100 μm, fretting occurred in a mixed slip regime (MSR) and gross slip regime (GSR). With continuous fretting wear, the dissipated energy of the material decreased gradually with increasing temperature in a stable state. The wear damage was mainly combined with fatigue wear and peeling. When the temperature was 100 ℃, the coefficient of friction (COF) value in the stable state was large. As the temperature increased, adhesive wear and oxidative wear gradually predominated in the fretting tests. The effect of high temperature easily formed an adhesive layer on the worn surface with wear reduction, especially at a high displacement amplitude. [ABSTRACT FROM AUTHOR]

Highlights: [•] Protection of Se-Car on hepatic toxicity induced by MC-LR examined in mice. [•] Potential mitochondrial dysfunction and endoplasmic reticulum stress were disclosed. [•] Se-Car firstly confers protection against damages induced by MC-LR. [ABSTRACT FROM AUTHOR]